Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90807.t1 | XP_003592777.1 | 94.1 | 238 | 14 | 0 | 69 | 306 | 214 | 451 | 2.30E-121 | 445.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90807.t1 | E2RBS6 | 28.6 | 119 | 79 | 2 | 49 | 162 | 246 | 363 | 6.2e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90807.t1 | G7IF12 | 94.1 | 238 | 14 | 0 | 69 | 306 | 214 | 451 | 1.7e-121 | 445.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene90807.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90807.t1 | MTR_1g115980 | 94.118 | 238 | 14 | 0 | 69 | 306 | 214 | 451 | 3.92e-157 | 446 |
| MS.gene90807.t1 | MTR_1g115980 | 91.176 | 238 | 12 | 1 | 69 | 306 | 214 | 442 | 2.03e-148 | 424 |
| MS.gene90807.t1 | MTR_1g115980 | 94.211 | 190 | 11 | 0 | 69 | 258 | 214 | 403 | 2.70e-129 | 375 |
| MS.gene90807.t1 | MTR_1g115980 | 90.526 | 190 | 9 | 1 | 69 | 258 | 214 | 394 | 8.12e-121 | 353 |
| MS.gene90807.t1 | MTR_1g115980 | 92.517 | 147 | 11 | 0 | 69 | 215 | 214 | 360 | 3.50e-97 | 291 |
| MS.gene90807.t1 | MTR_1g115980 | 87.755 | 147 | 9 | 1 | 69 | 215 | 214 | 351 | 1.37e-88 | 269 |
| MS.gene90807.t1 | MTR_3g082690 | 38.384 | 99 | 57 | 2 | 64 | 159 | 249 | 346 | 7.22e-12 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90807.t1 | AT3G55080 | 58.650 | 237 | 96 | 1 | 69 | 305 | 109 | 343 | 3.39e-96 | 288 |
| MS.gene90807.t1 | AT3G55080 | 58.650 | 237 | 96 | 1 | 69 | 305 | 140 | 374 | 1.02e-95 | 288 |
| MS.gene90807.t1 | AT3G55080 | 58.650 | 237 | 96 | 1 | 69 | 305 | 140 | 374 | 1.02e-95 | 288 |
| MS.gene90807.t1 | AT3G55080 | 58.650 | 237 | 96 | 1 | 69 | 305 | 224 | 458 | 7.21e-95 | 288 |
| MS.gene90807.t1 | AT3G55080 | 57.438 | 242 | 96 | 2 | 69 | 305 | 109 | 348 | 9.71e-94 | 282 |
Find 66 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATGCTCCGCAATTAAT+TGG | 0.158521 | 1.4:-88034772 | None:intergenic |
| CTCCGAGCCCTTTGCATCTT+TGG | 0.188607 | 1.4:-88033751 | None:intergenic |
| TCTTTCTTGTACCACTCTTC+AGG | 0.243444 | 1.4:+88034487 | MS.gene90807:CDS |
| TCCTCTGCGTCACACAAAAT+TGG | 0.272658 | 1.4:+88033698 | MS.gene90807:CDS |
| TTTCAGGTGCTTATAAGATA+TGG | 0.280416 | 1.4:+88033430 | MS.gene90807:intron |
| GCTGGCTAGGCGCCCTTTAC+AGG | 0.304269 | 1.4:+88034700 | MS.gene90807:CDS |
| GGAGCATAATGCAGCTATTA+TGG | 0.347092 | 1.4:+88034784 | MS.gene90807:CDS |
| AATCACTTCGTGCACTTGCT+CGG | 0.349523 | 1.4:+88034462 | MS.gene90807:CDS |
| TCTGATCGTGATTATGTTCC+TGG | 0.368828 | 1.4:+88027424 | MS.gene90807:CDS |
| CGAGCCATTCGTCTTCTAAC+AGG | 0.372330 | 1.4:-88035087 | None:intergenic |
| CTTTGTTCATATCCTGTAAA+GGG | 0.388424 | 1.4:-88034712 | None:intergenic |
| CCCTTTGCATCTTTGGTTGG+AGG | 0.393470 | 1.4:-88033744 | None:intergenic |
| GTGAAGTCTGCTGGAGGAAA+AGG | 0.398194 | 1.4:+88034425 | MS.gene90807:CDS |
| CTTTCTTGTACCACTCTTCA+GGG | 0.401046 | 1.4:+88034488 | MS.gene90807:CDS |
| GAGCCCTTTGCATCTTTGGT+TGG | 0.427651 | 1.4:-88033747 | None:intergenic |
| ATCACTTCGTGCACTTGCTC+GGG | 0.429403 | 1.4:+88034463 | MS.gene90807:CDS |
| ACCTGATCATAAATGTTGTA+TGG | 0.431705 | 1.4:-88033499 | None:intergenic |
| TCCATACAACATTTATGATC+AGG | 0.435626 | 1.4:+88033498 | MS.gene90807:CDS |
| AATGCCAGAGCTTAACCATT+TGG | 0.439954 | 1.4:+88034649 | MS.gene90807:intron |
| TGATTATGTTCCTGGTGAAC+AGG | 0.442198 | 1.4:+88027432 | MS.gene90807:CDS |
| CATAGGGAGGTGAAGTCTGC+TGG | 0.442386 | 1.4:+88034416 | MS.gene90807:intron |
| AAATTCCAAGGCAGTGTTTG+AGG | 0.446407 | 1.4:-88025335 | None:intergenic |
| TCTTTGTTCATATCCTGTAA+AGG | 0.450034 | 1.4:-88034713 | None:intergenic |
| TTCAAATGCTACATTGATGC+TGG | 0.452747 | 1.4:+88033459 | MS.gene90807:CDS |
| GCTACATTGATGCTGGACTT+TGG | 0.464448 | 1.4:+88033466 | MS.gene90807:CDS |
| CAAATCTGCTTCTACCTGGC+TGG | 0.469867 | 1.4:+88035140 | MS.gene90807:CDS |
| TTCAATTTCAAATGACCAGT+TGG | 0.470814 | 1.4:+88026687 | MS.gene90807:CDS |
| AAACTGATGGTAGGCTGGCT+AGG | 0.476607 | 1.4:+88034687 | MS.gene90807:CDS |
| TGAAACCTCAAACACTGCCT+TGG | 0.479315 | 1.4:+88025330 | MS.gene90807:CDS |
| CAGATTTCTTGAATCATGAC+GGG | 0.480634 | 1.4:+88026832 | MS.gene90807:CDS |
| AACCAAAGATGCAAAGGGCT+CGG | 0.490231 | 1.4:+88033749 | MS.gene90807:CDS |
| CCCCATGCTCTAGAGCCAAC+TGG | 0.493834 | 1.4:-88026702 | None:intergenic |
| GTGCAGCCTCCGTGACCAAA+TGG | 0.495854 | 1.4:-88034664 | None:intergenic |
| CATACTTAGGTATATCGTAC+TGG | 0.499200 | 1.4:-88033674 | None:intergenic |
| GACCAGTTGGCTCTAGAGCA+TGG | 0.502799 | 1.4:+88026700 | MS.gene90807:CDS |
| TGCACAAACTGATGGTAGGC+TGG | 0.508432 | 1.4:+88034682 | MS.gene90807:CDS |
| ACCAGTTGGCTCTAGAGCAT+GGG | 0.517262 | 1.4:+88026701 | MS.gene90807:CDS |
| AGAGCATGGGGAAGCACAAA+GGG | 0.532315 | 1.4:+88026714 | MS.gene90807:CDS |
| TTCTCAAATCTGCTTCTACC+TGG | 0.536097 | 1.4:+88035136 | MS.gene90807:CDS |
| TGAAGTCTGCTGGAGGAAAA+GGG | 0.538297 | 1.4:+88034426 | MS.gene90807:CDS |
| GCCTCCAACCAAAGATGCAA+AGG | 0.545403 | 1.4:+88033743 | MS.gene90807:CDS |
| TAGTATTTGACCTGTTCACC+AGG | 0.550072 | 1.4:-88027442 | None:intergenic |
| GCAGATTTCTTGAATCATGA+CGG | 0.555755 | 1.4:+88026831 | MS.gene90807:CDS |
| AATTAATTGGATGAATAATG+AGG | 0.556334 | 1.4:-88034759 | None:intergenic |
| GCAAGTGCACGAAGTGATTG+TGG | 0.565446 | 1.4:-88034458 | None:intergenic |
| ATTATTCATCCAATTAATTG+CGG | 0.565451 | 1.4:+88034763 | MS.gene90807:CDS |
| CCTCCAACCAAAGATGCAAA+GGG | 0.571025 | 1.4:+88033744 | MS.gene90807:CDS |
| AACAGGAAGTCTTTCACTCG+AGG | 0.574466 | 1.4:-88035070 | None:intergenic |
| TGACGCAGAGGATCATACTT+AGG | 0.583198 | 1.4:-88033687 | None:intergenic |
| GTCTGCTGGAGGAAAAGGGA+AGG | 0.583307 | 1.4:+88034430 | MS.gene90807:CDS |
| AAAACAGTAGTTCTCCAGCC+AGG | 0.585984 | 1.4:-88035154 | None:intergenic |
| CAAGACATCAACGACGTGAG+AGG | 0.593044 | 1.4:-88025272 | None:intergenic |
| ACGGAGGCTGCACAAACTGA+TGG | 0.603336 | 1.4:+88034674 | MS.gene90807:CDS |
| AAGTGCAAACTTGATTGTGA+AGG | 0.605155 | 1.4:-88033787 | None:intergenic |
| TAGAGCATGGGGAAGCACAA+AGG | 0.605458 | 1.4:+88026713 | MS.gene90807:CDS |
| TACAATGGCAACAAATTCCA+AGG | 0.607933 | 1.4:-88025347 | None:intergenic |
| AGAGCTTAACCATTTGGTCA+CGG | 0.610389 | 1.4:+88034655 | MS.gene90807:intron |
| AGGCTGCACAAACTGATGGT+AGG | 0.613383 | 1.4:+88034678 | MS.gene90807:CDS |
| CCAGTTGGCTCTAGAGCATG+GGG | 0.627133 | 1.4:+88026702 | MS.gene90807:CDS |
| TGTTTACACACCCTGAAGAG+TGG | 0.628835 | 1.4:-88034498 | None:intergenic |
| ACTTCCTGTTAGAAGACGAA+TGG | 0.634423 | 1.4:+88035083 | MS.gene90807:CDS |
| ATTTACTGACCATCAAGTCA+AGG | 0.654900 | 1.4:-88035178 | None:intergenic |
| TCTGCTGGAGGAAAAGGGAA+GGG | 0.677166 | 1.4:+88034431 | MS.gene90807:CDS |
| AGGGAGGTGAAGTCTGCTGG+AGG | 0.687293 | 1.4:+88034419 | MS.gene90807:intron |
| ATGGCTCGAGATCTCTTGCA+CGG | 0.704607 | 1.4:+88035102 | MS.gene90807:CDS |
| GCTTAACCATTTGGTCACGG+AGG | 0.739786 | 1.4:+88034658 | MS.gene90807:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 88025226 | 88035188 | 88025226 | ID=MS.gene90807 |
| chr1.4 | mRNA | 88025226 | 88035188 | 88025226 | ID=MS.gene90807.t1;Parent=MS.gene90807 |
| chr1.4 | exon | 88025226 | 88025366 | 88025226 | ID=MS.gene90807.t1.exon1;Parent=MS.gene90807.t1 |
| chr1.4 | CDS | 88025226 | 88025366 | 88025226 | ID=cds.MS.gene90807.t1;Parent=MS.gene90807.t1 |
| chr1.4 | exon | 88026673 | 88026735 | 88026673 | ID=MS.gene90807.t1.exon2;Parent=MS.gene90807.t1 |
| chr1.4 | CDS | 88026673 | 88026735 | 88026673 | ID=cds.MS.gene90807.t1;Parent=MS.gene90807.t1 |
| chr1.4 | exon | 88026822 | 88026902 | 88026822 | ID=MS.gene90807.t1.exon3;Parent=MS.gene90807.t1 |
| chr1.4 | CDS | 88026822 | 88026902 | 88026822 | ID=cds.MS.gene90807.t1;Parent=MS.gene90807.t1 |
| chr1.4 | exon | 88027418 | 88027453 | 88027418 | ID=MS.gene90807.t1.exon4;Parent=MS.gene90807.t1 |
| chr1.4 | CDS | 88027418 | 88027453 | 88027418 | ID=cds.MS.gene90807.t1;Parent=MS.gene90807.t1 |
| chr1.4 | exon | 88033436 | 88033519 | 88033436 | ID=MS.gene90807.t1.exon5;Parent=MS.gene90807.t1 |
| chr1.4 | CDS | 88033436 | 88033519 | 88033436 | ID=cds.MS.gene90807.t1;Parent=MS.gene90807.t1 |
| chr1.4 | exon | 88033666 | 88033799 | 88033666 | ID=MS.gene90807.t1.exon6;Parent=MS.gene90807.t1 |
| chr1.4 | CDS | 88033666 | 88033799 | 88033666 | ID=cds.MS.gene90807.t1;Parent=MS.gene90807.t1 |
| chr1.4 | exon | 88034421 | 88034509 | 88034421 | ID=MS.gene90807.t1.exon7;Parent=MS.gene90807.t1 |
| chr1.4 | CDS | 88034421 | 88034509 | 88034421 | ID=cds.MS.gene90807.t1;Parent=MS.gene90807.t1 |
| chr1.4 | exon | 88034657 | 88034805 | 88034657 | ID=MS.gene90807.t1.exon8;Parent=MS.gene90807.t1 |
| chr1.4 | CDS | 88034657 | 88034805 | 88034657 | ID=cds.MS.gene90807.t1;Parent=MS.gene90807.t1 |
| chr1.4 | exon | 88035045 | 88035188 | 88035045 | ID=MS.gene90807.t1.exon9;Parent=MS.gene90807.t1 |
| chr1.4 | CDS | 88035045 | 88035188 | 88035045 | ID=cds.MS.gene90807.t1;Parent=MS.gene90807.t1 |
| Gene Sequence |
| Protein sequence |
