AlfalfaGEDB | MS.gene90989

M. sativa cultivar XinJiangDaYe / MS.gene90989

KEGG: K09419
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90989.t1	XP_013443333.1	94.4	341	19	0	1	341	1	341	1.90E-156	562

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90989.t1	Q9SCW4	54.5	321	102	6	26	340	16	298	8.6e-57	222.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90989.t1	A0A072TKJ1	94.4	341	19	0	1	341	1	341	1.4e-156	562.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene90989.t1	TF	HSF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90989.t1	MTR_0020s0020	94.135	341	20	0	1	341	1	341	0.0	582
MS.gene90989.t1	MTR_1g100777	54.493	345	109	10	1	340	1	302	4.60e-106	313
MS.gene90989.t1	MTR_5g010680	43.548	372	134	8	7	325	5	353	4.87e-80	248
MS.gene90989.t1	MTR_3g101870	45.205	219	102	3	23	227	14	228	8.65e-59	194
MS.gene90989.t1	MTR_5g089170	38.148	270	126	7	23	286	15	249	6.31e-53	175
MS.gene90989.t1	MTR_5g017470	44.091	220	81	4	26	230	2	194	4.50e-51	171
MS.gene90989.t1	MTR_7g091370	43.000	200	78	2	27	226	16	179	6.93e-45	154
MS.gene90989.t1	MTR_5g029680	61.111	108	42	0	17	124	16	123	1.70e-41	151
MS.gene90989.t1	MTR_7g095680	51.220	123	59	1	3	124	18	140	4.32e-40	144
MS.gene90989.t1	MTR_6g043060	49.606	127	64	0	32	158	21	147	1.75e-39	142
MS.gene90989.t1	MTR_1g071360	54.955	111	50	0	31	141	30	140	7.28e-39	141
MS.gene90989.t1	MTR_1g071360	54.955	111	50	0	31	141	27	137	1.21e-38	141
MS.gene90989.t1	MTR_4g077970	65.217	92	32	0	33	124	12	103	2.95e-38	142
MS.gene90989.t1	MTR_1g102860	61.702	94	36	0	31	124	38	131	8.61e-37	135
MS.gene90989.t1	MTR_8g083100	49.612	129	65	0	34	162	23	151	1.29e-36	138
MS.gene90989.t1	MTR_8g087540	59.596	99	39	1	26	124	6	103	1.54e-36	136
MS.gene90989.t1	MTR_1g061170	52.101	119	51	1	33	145	49	167	1.59e-36	137
MS.gene90989.t1	MTR_4g088900	36.585	205	92	5	31	231	31	201	6.52e-36	129
MS.gene90989.t1	MTR_2g100680	58.491	106	43	1	31	135	29	134	1.06e-35	135
MS.gene90989.t1	MTR_3g104550	58.511	94	39	0	31	124	44	137	1.01e-34	130
MS.gene90989.t1	MTR_6g086805	57.143	98	42	0	27	124	5	102	5.84e-34	128
MS.gene90989.t1	MTR_2g017890	48.739	119	61	0	33	151	15	133	2.72e-33	127
MS.gene90989.t1	MTR_2g100670	52.830	106	49	1	31	135	31	136	1.46e-32	123
MS.gene90989.t1	MTR_2g100670	55.319	94	42	0	31	124	139	232	1.20e-31	123
MS.gene90989.t1	MTR_8g105780	59.574	94	37	1	32	124	21	114	3.26e-31	121
MS.gene90989.t1	MTR_4g045757	56.897	58	25	0	33	90	14	71	2.12e-17	76.3
MS.gene90989.t1	MTR_5g047190	41.758	91	46	2	41	124	1	91	1.03e-15	72.4
MS.gene90989.t1	MTR_4g011850	29.630	135	49	2	93	227	9	97	6.65e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90989.t1	AT5G62020	47.619	336	102	9	26	340	16	298	2.79e-87	265
MS.gene90989.t1	AT4G11660	46.243	346	122	8	25	329	51	373	7.84e-79	246
MS.gene90989.t1	AT1G46264	47.783	203	102	2	28	227	29	230	1.23e-56	187
MS.gene90989.t1	AT4G36990	42.857	217	84	3	21	226	2	189	2.78e-51	172
MS.gene90989.t1	AT2G41690	43.939	198	93	3	31	228	38	217	1.10e-44	153
MS.gene90989.t1	AT4G17750	63.725	102	37	0	23	124	42	143	1.89e-40	148
MS.gene90989.t1	AT3G22830	60.000	110	44	0	15	124	43	152	4.64e-40	145
MS.gene90989.t1	AT1G32330	67.021	94	31	0	31	124	35	128	1.03e-39	146
MS.gene90989.t1	AT1G32330	67.021	94	31	0	31	124	35	128	1.11e-39	146
MS.gene90989.t1	AT3G02990	66.667	96	31	1	29	124	20	114	8.32e-39	143
MS.gene90989.t1	AT5G43840	59.223	103	42	0	31	133	17	119	1.15e-38	139
MS.gene90989.t1	AT3G51910	55.340	103	46	0	31	133	27	129	3.87e-38	137
MS.gene90989.t1	AT5G16820	65.625	96	32	1	29	124	24	118	5.77e-37	139
MS.gene90989.t1	AT5G16820	65.625	96	32	1	29	124	24	118	5.77e-37	139
MS.gene90989.t1	AT2G26150	59.574	94	38	0	31	124	42	135	2.83e-36	134
MS.gene90989.t1	AT2G26150	59.574	94	38	0	31	124	42	135	2.83e-36	134
MS.gene90989.t1	AT3G63350	56.701	97	42	0	27	123	22	118	5.68e-36	132
MS.gene90989.t1	AT5G03720	40.984	183	61	2	33	215	55	190	5.91e-36	135
MS.gene90989.t1	AT5G03720	40.984	183	61	2	33	215	55	190	5.91e-36	135
MS.gene90989.t1	AT5G54070	55.660	106	43	1	30	131	68	173	3.70e-35	131
MS.gene90989.t1	AT4G13980	57.843	102	42	1	34	134	24	125	8.90e-35	132
MS.gene90989.t1	AT3G24520	61.053	95	37	0	30	124	14	108	1.30e-34	129
MS.gene90989.t1	AT1G67970	39.634	164	80	3	33	195	19	164	1.16e-31	122
MS.gene90989.t1	AT5G45710	56.757	111	44	2	29	139	10	116	3.07e-29	115
MS.gene90989.t1	AT5G45710	56.757	111	44	2	29	139	10	116	3.07e-29	115
MS.gene90989.t1	AT4G18880	59.596	99	39	1	26	124	9	106	4.48e-28	113
MS.gene90989.t1	AT4G18870	46.067	89	43	3	34	120	14	99	7.97e-19	85.9
MS.gene90989.t1	AT4G18870	42.391	92	50	1	29	117	139	230	1.51e-18	85.1
MS.gene90989.t1	AT1G77570	39.773	88	48	3	34	117	27	113	6.31e-11	60.5

Find 0 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATATGTTTTTGTCAGAAA+CGG	+	8272:103816-103835	None:intergenic	20.0%
!!!	TTATTGTGTTTGAAATACTT+TGG	+	8272:103939-103958	None:intergenic	20.0%
!!!	GTTTTGTTGATTTTTTTGCA+GGG	-	8272:104106-104125	MS.gene90989:CDS	25.0%
!!!	TGTTTTGTTGATTTTTTTGC+AGG	-	8272:104105-104124	MS.gene90989:CDS	25.0%
	AAATTACTAGCAAAGATGGT+GGG	+	8272:103912-103931	None:intergenic	30.0%
	AACAATAACACTCAAACAGA+CGG	-	8272:104538-104557	MS.gene90989:intron	30.0%
	AAGCAAATTACTAGCAAAGA+TGG	+	8272:103916-103935	None:intergenic	30.0%
!!	ATGGATCCACTTTTATTGTT+TGG	-	8272:103883-103902	MS.gene90989:CDS	30.0%
	AATTACTAGCAAAGATGGTG+GGG	+	8272:103911-103930	None:intergenic	35.0%
	ACTCATTTGAAAATTCCCAC+CGG	+	8272:104153-104172	None:intergenic	35.0%
	AGAGAAAACGGTTGTGAAAA+TGG	-	8272:104706-104725	MS.gene90989:CDS	35.0%
	CAAATTACTAGCAAAGATGG+TGG	+	8272:103913-103932	None:intergenic	35.0%
	GAAATGAATCCGAAGCTATT+CGG	-	8272:104658-104677	MS.gene90989:CDS	35.0%
	GCAAAACGAGTAAGAGAAAA+CGG	-	8272:104694-104713	MS.gene90989:CDS	35.0%
	TCAAATGAGTATTTCCGAAG+AGG	-	8272:104163-104182	MS.gene90989:CDS	35.0%
	TCATGTTCTTCACAATCCAA+CGG	+	8272:104796-104815	None:intergenic	35.0%
	TTAGAAAAGTAGTACCTGAC+CGG	-	8272:104131-104150	MS.gene90989:CDS	35.0%
	TTGACGAATTGAAGCGATAA+GGG	-	8272:104069-104088	MS.gene90989:CDS	35.0%
	TTTGACGAATTGAAGCGATA+AGG	-	8272:104068-104087	MS.gene90989:CDS	35.0%
!!	AATAGATCTCTGAGACTCAA+GGG	+	8272:103787-103806	None:intergenic	35.0%
	AAGTAGAGAATCCGGTATGA+CGG	-	8272:104573-104592	MS.gene90989:intron	40.0%
	AATAACACTCAAACAGACGG+TGG	-	8272:104541-104560	MS.gene90989:intron	40.0%
	ACTGTTGACGGTAATACCAA+CGG	-	8272:104321-104340	MS.gene90989:CDS	40.0%
	ATGATTTCACCGTCAAACTC+CGG	-	8272:104352-104371	MS.gene90989:CDS	40.0%
	ATTTCCGAAGAGGAGAAAAG+CGG	-	8272:104173-104192	MS.gene90989:CDS	40.0%
	CGATAGATTTATCATCGACG+AGG	+	8272:103839-103858	None:intergenic	40.0%
	CGATGATAAATCTATCGACG+AGG	-	8272:103842-103861	MS.gene90989:CDS	40.0%
	GAGTTTGACGGTGAAATCAT+CGG	+	8272:104352-104371	None:intergenic	40.0%
	GGCTTCTATGTGAGATTCAA+CGG	-	8272:104194-104213	MS.gene90989:CDS	40.0%
	GGGGTTCCAAACAATAAAAG+TGG	+	8272:103892-103911	None:intergenic	40.0%
	TTCTATGTGAGATTCAACGG+AGG	-	8272:104197-104216	MS.gene90989:CDS	40.0%
!	ATGATAATGATCGAACAGCG+TGG	-	8272:104812-104831	MS.gene90989:CDS	40.0%
!	GTTATTTCGTGGAACGAAGA+TGG	-	8272:103864-103883	MS.gene90989:CDS	40.0%
!	GTTTTTGTCAGAAACGGTGT+AGG	+	8272:103810-103829	None:intergenic	40.0%
!!	ATTGAGTTGACGAACAAAGC+TGG	+	8272:103967-103986	None:intergenic	40.0%
!!	GAATAGATCTCTGAGACTCA+AGG	+	8272:103788-103807	None:intergenic	40.0%
	AAAAGTAGTACCTGACCGGT+GGG	-	8272:104135-104154	MS.gene90989:CDS	45.0%
	AGACGTGAAATCTGAACCGT+TGG	-	8272:104777-104796	MS.gene90989:CDS	45.0%
	AGCAGAAACTACAACAGCGA+CGG	-	8272:104279-104298	MS.gene90989:CDS	45.0%
	CGATATCACCTGTTCTTCAC+CGG	+	8272:104374-104393	None:intergenic	45.0%
	GATATCGTCGAGTTCTTCAC+CGG	-	8272:104387-104406	MS.gene90989:CDS	45.0%
	GATTTCACGTCTGTAGAACC+AGG	+	8272:104769-104788	None:intergenic	45.0%
	GCTCAAGGAAGTAGAGAATC+CGG	-	8272:104565-104584	MS.gene90989:intron	45.0%
	GGTATTACCGTCAACAGTAC+CGG	+	8272:104319-104338	None:intergenic	45.0%
	TGTTCTTCACCGGAGTTTGA+CGG	+	8272:104364-104383	None:intergenic	45.0%
	TTTGAAAATTCCCACCGGTC+AGG	+	8272:104148-104167	None:intergenic	45.0%
!	ATCAAATCAAGCGGCGGATT+TGG	+	8272:104607-104626	None:intergenic	45.0%
!	CTATCGACGAGGTTATTTCG+TGG	-	8272:103853-103872	MS.gene90989:CDS	45.0%
!!	CTGTAGAACCAGGTTGATGT+AGG	+	8272:104759-104778	None:intergenic	45.0%
!!	GTTTCTCCGTTGTTTTCCAC+CGG	+	8272:103738-103757	None:intergenic	45.0%
!!	AAAGAAAATAATATAATAAT+AGG	+	8272:104014-104033	None:intergenic	5.0%
	AGCTTCTCGACGAAAACGAG+AGG	-	8272:104425-104444	MS.gene90989:CDS	50.0%
	ATCGCCACACCGAATAGCTT+CGG	+	8272:104670-104689	None:intergenic	50.0%
	CTTCGGATTCATTTCCTCGC+CGG	+	8272:104653-104672	None:intergenic	50.0%
	GAAAAGTAGTACCTGACCGG+TGG	-	8272:104134-104153	MS.gene90989:CDS	50.0%
	GCAGAAACAGACACAGACGA+CGG	+	8272:104247-104266	None:intergenic	50.0%
	GTCAAACTCCGGTGAAGAAC+AGG	-	8272:104363-104382	MS.gene90989:CDS	50.0%
	TAGAGAATCCGGTATGACGG+CGG	-	8272:104576-104595	MS.gene90989:intron	50.0%
	TCCTTCACCGGTACTGTTGA+CGG	-	8272:104309-104328	MS.gene90989:CDS	50.0%
	TCGACGAAAACGAGAGGTTG+AGG	-	8272:104431-104450	MS.gene90989:CDS	50.0%
!	CTCGTTTTCGTCGAGAAGCT+CGG	+	8272:104425-104444	None:intergenic	50.0%
!	GAATCCGAAGCTATTCGGTG+TGG	-	8272:104663-104682	MS.gene90989:CDS	50.0%
!!	AAGCTATTCGGTGTGGCGAT+TGG	-	8272:104670-104689	MS.gene90989:CDS	50.0%
!!	AGATCTCTGAGACTCAAGGG+TGG	+	8272:103784-103803	None:intergenic	50.0%
!!	GATCTCTGAGACTCAAGGGT+GGG	+	8272:103783-103802	None:intergenic	50.0%
!!!	GAAGCCGCTTTTCTCCTCTT+CGG	+	8272:104180-104199	None:intergenic	50.0%
	ACAGACACAGACGACGGTGA+AGG	+	8272:104241-104260	None:intergenic	55.0%
	ACCGCCGGCATCAAATCAAG+CGG	+	8272:104616-104635	None:intergenic	55.0%
	CAAACAGACGGTGGTGCTCA+AGG	-	8272:104550-104569	MS.gene90989:intron	55.0%
	CCAGCTCCGGTGGAAAACAA+CGG	-	8272:103729-103748	MS.gene90989:CDS	55.0%
	CTGTTGCGCCTACATCAACC+TGG	-	8272:104748-104767	MS.gene90989:CDS	55.0%
	GAAATCATCGGCATCGCCGT+TGG	+	8272:104340-104359	None:intergenic	55.0%
	GACGGTTGCAGTTCCTTCAC+CGG	-	8272:104297-104316	MS.gene90989:CDS	55.0%
	TGGATTCACCGCCGTCATAC+CGG	+	8272:104587-104606	None:intergenic	55.0%
!	ACAGACGACGGTGAAGGTGT+TGG	+	8272:104235-104254	None:intergenic	55.0%
!	CTCAAGGGTGGGTGTTGCAA+CGG	+	8272:103772-103791	None:intergenic	55.0%
!	TCCGCCGCTTGATTTGATGC+CGG	-	8272:104609-104628	MS.gene90989:CDS	55.0%
!	TTTTCCACCGGAGCTGGTGA+AGG	+	8272:103726-103745	None:intergenic	55.0%
!	TTTTCGTCGAGAAGCTCGGC+CGG	+	8272:104421-104440	None:intergenic	55.0%
!!	ACCGTCAACAGTACCGGTGA+AGG	+	8272:104313-104332	None:intergenic	55.0%
	GCCGGCATCAAATCAAGCGG+CGG	+	8272:104613-104632	None:intergenic	60.0%
	TGATGCCGGCGGTGGCAAAA+CGG	-	8272:104623-104642	MS.gene90989:CDS	60.0%
	TTCTTCACCGGCACGTGCAC+CGG	-	8272:104399-104418	MS.gene90989:CDS	60.0%
!	AAGGGTGGGTGTTGCAACGG+TGG	+	8272:103769-103788	None:intergenic	60.0%
!	GCCGCTTGATTTGATGCCGG+CGG	-	8272:104612-104631	MS.gene90989:CDS	60.0%
!	GCTTGATTTGATGCCGGCGG+TGG	-	8272:104615-104634	MS.gene90989:CDS	60.0%
!	GGGTGTTGCAACGGTGGTTG+TGG	+	8272:103763-103782	None:intergenic	60.0%
!!	CCGTTGTTTTCCACCGGAGC+TGG	+	8272:103732-103751	None:intergenic	60.0%
	GCGGTGGCAAAACGGAGCTC+CGG	-	8272:104631-104650	MS.gene90989:CDS	65.0%
!	GAGCTCCGTTTTGCCACCGC+CGG	+	8272:104631-104650	None:intergenic	65.0%
	GGCAAAACGGAGCTCCGGCG+AGG	-	8272:104636-104655	MS.gene90989:CDS	70.0%
	GGCTCCTTCACCAGCTCCGG+TGG	-	8272:103719-103738	MS.gene90989:CDS	70.0%
	AGCTCGGCCGGTGCACGTGC+CGG	+	8272:104409-104428	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
8272	gene	103717	104876	103717	ID=MS.gene90989
8272	mRNA	103717	104876	103717	ID=MS.gene90989.t1;Parent=MS.gene90989
8272	exon	104601	104876	104601	ID=MS.gene90989.t1.exon1;Parent=MS.gene90989.t1
8272	CDS	104601	104876	104601	ID=cds.MS.gene90989.t1;Parent=MS.gene90989.t1
8272	exon	103717	104466	103717	ID=MS.gene90989.t1.exon2;Parent=MS.gene90989.t1
8272	CDS	103717	104466	103717	ID=cds.MS.gene90989.t1;Parent=MS.gene90989.t1

Gene Sequence

>MS.gene90989

ATGGCTCCTTCACCAGCTCCGGTGGAAAACAACGGAGAAACAACCACAACCACCGTTGCAACACCCACCCTTGAGTCTCAGAGATCTATTCCTACACCGTTTCTGACAAAAACATATAACCTCGTCGATGATAAATCTATCGACGAGGTTATTTCGTGGAACGAAGATGGATCCACTTTTATTGTTTGGAACCCCACCATCTTTGCTAGTAATTTGCTTCCAAAGTATTTCAAACACAATAATTTCTCCAGCTTTGTTCGTCAACTCAATACTTACGTAAATTCAACTTTCTTTCCTATTATTATATTATTTTCTTTTATGCTTGTTTTTGTATGAATTTTAATTAATATTTTTGACGAATTGAAGCGATAAGGGCTGAATTTTAACTTGTTTTGTTGATTTTTTTGCAGGGATTTAGAAAAGTAGTACCTGACCGGTGGGAATTTTCAAATGAGTATTTCCGAAGAGGAGAAAAGCGGCTTCTATGTGAGATTCAACGGAGGAAGATCTGTTCTCCAACACCTTCACCGTCGTCTGTGTCTGTTTCTGCTGCTGTAACAGCAGCAGAAACTACAACAGCGACGGTTGCAGTTCCTTCACCGGTACTGTTGACGGTAATACCAACGGCGATGCCGATGATTTCACCGTCAAACTCCGGTGAAGAACAGGTGATATCGTCGAGTTCTTCACCGGCACGTGCACCGGCCGAGCTTCTCGACGAAAACGAGAGGTTGAGGAAAGAGAACTCTCTATTGAATAAAGAACTTGCTGAGATGAGATTGCTTTGTAACAATATATACTCTCTGATGTCAAATTTTGCAAACAATAACACTCAAACAGACGGTGGTGCTCAAGGAAGTAGAGAATCCGGTATGACGGCGGTGAATCCAAATCCGCCGCTTGATTTGATGCCGGCGGTGGCAAAACGGAGCTCCGGCGAGGAAATGAATCCGAAGCTATTCGGTGTGGCGATTGGAGCAAAACGAGTAAGAGAAAACGGTTGTGAAAATGGAGAAAGAGAAGATGATACACTGTTGCGCCTACATCAACCTGGTTCTACAGACGTGAAATCTGAACCGTTGGATTGTGAAGAACATGATAATGATCGAACAGCGTGGTTGAATCAGAGTCGTAGAAAGAATCAGAATTTGTGTAAATGA

Protein sequence

>MS.gene90989.t1

MAPSPAPVENNGETTTTTVATPTLESQRSIPTPFLTKTYNLVDDKSIDEVISWNEDGSTFIVWNPTIFASNLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFSNEYFRRGEKRLLCEIQRRKICSPTPSPSSVSVSAAVTAAETTTATVAVPSPVLLTVIPTAMPMISPSNSGEEQVISSSSSPARAPAELLDENERLRKENSLLNKELAEMRLLCNNIYSLMSNFANNNTQTDGGAQGSRESGMTAVNPNPPLDLMPAVAKRSSGEEMNPKLFGVAIGAKRVRENGCENGEREDDTLLRLHQPGSTDVKSEPLDCEEHDNDRTAWLNQSRRKNQNLCK