AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91193

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91193.t1 MTR_1g008290 92.143 140 8 1 86 225 8 144 2.99e-90 276
MS.gene91193.t1 MTR_4g035820 29.442 394 218 12 33 403 75 431 1.36e-33 136
MS.gene91193.t1 MTR_5g032260 66.265 83 28 0 84 166 323 405 4.93e-31 125
MS.gene91193.t1 MTR_7g446520 30.252 238 146 6 4 240 66 284 5.80e-24 103
MS.gene91193.t1 MTR_1g070355 32.558 215 126 8 38 240 79 286 3.09e-23 104
MS.gene91193.t1 MTR_0402s0040 30.168 179 120 2 33 206 26 204 3.67e-23 103
MS.gene91193.t1 MTR_6g051425 24.000 225 162 3 25 240 9 233 1.83e-20 93.6
MS.gene91193.t1 MTR_5g076070 26.877 253 160 9 7 240 19 265 5.94e-18 85.1
MS.gene91193.t1 MTR_7g446480 34.921 126 81 1 116 240 152 277 8.09e-17 82.4
MS.gene91193.t1 MTR_7g446510 33.858 127 83 1 116 241 167 293 5.72e-16 80.1
MS.gene91193.t1 MTR_1g013060 28.095 210 136 7 32 229 23 229 9.90e-15 74.3
MS.gene91193.t1 MTR_3g006370 33.600 125 73 3 17 131 29 153 1.23e-14 73.9
MS.gene91193.t1 MTR_2g098880 30.000 130 82 2 8 131 51 177 5.90e-14 72.8
MS.gene91193.t1 MTR_2g098880 30.000 130 82 2 8 131 39 165 6.12e-14 72.4
MS.gene91193.t1 MTR_6g022070 29.104 134 94 1 256 388 34 167 1.39e-13 72.8
MS.gene91193.t1 MTR_4g067290 34.483 116 71 2 22 133 45 159 7.50e-13 68.9
MS.gene91193.t1 MTR_4g067290 34.483 116 71 2 22 133 45 159 8.11e-13 68.9
MS.gene91193.t1 MTR_4g067290 34.483 116 71 2 22 133 65 179 1.02e-12 68.9
MS.gene91193.t1 MTR_4g067290 34.483 116 71 2 22 133 63 177 1.08e-12 68.9
MS.gene91193.t1 MTR_4g067290 34.483 116 71 2 22 133 65 179 1.09e-12 68.9
MS.gene91193.t1 MTR_4g067290 34.483 116 71 2 22 133 105 219 1.57e-12 68.9
MS.gene91193.t1 MTR_2g098920 31.298 131 78 3 31 150 78 207 2.32e-12 68.2
MS.gene91193.t1 MTR_8g465780 35.922 103 65 1 295 396 1 103 2.34e-12 66.2
MS.gene91193.t1 MTR_1g083640 23.729 236 156 5 29 240 47 282 1.24e-11 66.2
MS.gene91193.t1 MTR_5g015480 30.539 167 97 7 3 160 31 187 8.40e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91193.t1 AT4G15090 40.691 376 207 5 242 612 370 734 5.11e-95 310
MS.gene91193.t1 AT4G15090 37.023 262 145 5 20 276 42 288 8.05e-52 191
MS.gene91193.t1 AT4G15090 40.691 376 207 5 242 612 370 734 5.11e-95 310
MS.gene91193.t1 AT4G15090 37.023 262 145 5 20 276 42 288 8.05e-52 191
MS.gene91193.t1 AT4G15090 40.691 376 207 5 242 612 370 734 5.11e-95 310
MS.gene91193.t1 AT4G15090 37.023 262 145 5 20 276 42 288 8.05e-52 191
MS.gene91193.t1 AT4G15090 40.691 376 207 5 242 612 370 734 5.11e-95 310
MS.gene91193.t1 AT4G15090 37.023 262 145 5 20 276 42 288 8.05e-52 191
MS.gene91193.t1 AT4G15090 40.691 376 207 5 242 612 370 734 5.11e-95 310
MS.gene91193.t1 AT4G15090 37.023 262 145 5 20 276 42 288 8.05e-52 191
MS.gene91193.t1 AT3G22170 47.855 303 157 1 242 544 379 680 2.31e-93 306
MS.gene91193.t1 AT3G22170 40.708 226 95 3 32 240 69 272 2.25e-47 179
MS.gene91193.t1 AT3G22170 47.855 303 157 1 242 544 379 680 2.31e-93 306
MS.gene91193.t1 AT3G22170 40.708 226 95 3 32 240 69 272 2.25e-47 179
MS.gene91193.t1 AT1G76320 45.033 302 164 1 242 543 307 606 2.27e-91 296
MS.gene91193.t1 AT1G76320 44.878 205 107 2 37 240 1 200 1.71e-48 180
MS.gene91193.t1 AT1G76320 45.033 302 164 1 242 543 307 606 6.73e-91 297
MS.gene91193.t1 AT1G76320 44.878 205 107 2 37 240 1 200 2.20e-48 181
MS.gene91193.t1 AT1G76320 45.033 302 164 1 242 543 307 606 6.73e-91 297
MS.gene91193.t1 AT1G76320 44.878 205 107 2 37 240 1 200 2.20e-48 181
MS.gene91193.t1 AT1G76320 45.033 302 164 1 242 543 307 606 8.37e-91 296
MS.gene91193.t1 AT1G76320 44.878 205 107 2 37 240 1 200 2.42e-48 181
MS.gene91193.t1 AT4G19990 44.781 297 161 2 240 533 259 555 1.59e-73 249
MS.gene91193.t1 AT4G19990 44.781 297 161 2 240 533 316 612 3.23e-73 250
MS.gene91193.t1 AT4G19990 34.348 230 99 4 28 240 16 210 8.60e-30 125
MS.gene91193.t1 AT4G19990 44.781 297 161 2 240 533 316 612 3.25e-73 250
MS.gene91193.t1 AT4G19990 34.348 230 99 4 28 240 16 210 7.34e-30 125
MS.gene91193.t1 AT4G19990 44.781 297 161 2 240 533 316 612 3.25e-73 250
MS.gene91193.t1 AT4G19990 34.348 230 99 4 28 240 16 210 7.34e-30 125
MS.gene91193.t1 AT4G19990 44.781 297 161 2 240 533 330 626 3.96e-73 250
MS.gene91193.t1 AT4G19990 34.348 230 99 4 28 240 30 224 7.50e-30 125
MS.gene91193.t1 AT4G19990 44.781 297 161 2 240 533 316 612 4.71e-73 250
MS.gene91193.t1 AT4G19990 34.348 230 99 4 28 240 16 210 1.01e-29 125
MS.gene91193.t1 AT4G19990 44.781 297 161 2 240 533 316 612 4.71e-73 250
MS.gene91193.t1 AT4G19990 34.348 230 99 4 28 240 16 210 1.01e-29 125
MS.gene91193.t1 AT4G38180 35.873 315 189 4 244 548 396 707 2.43e-64 226
MS.gene91193.t1 AT4G38180 35.111 225 137 3 32 248 72 295 5.54e-35 141
MS.gene91193.t1 AT2G32250 36.452 310 189 2 238 543 310 615 4.15e-64 225
MS.gene91193.t1 AT2G32250 58.163 98 40 1 36 132 41 138 1.43e-28 122
MS.gene91193.t1 AT2G32250 36.452 310 189 2 238 543 269 574 6.05e-64 225
MS.gene91193.t1 AT2G32250 57.732 97 40 1 37 132 1 97 1.10e-27 119
MS.gene91193.t1 AT2G32250 36.452 310 189 2 238 543 310 615 6.16e-64 225
MS.gene91193.t1 AT2G32250 58.163 98 40 1 36 132 41 138 1.60e-28 122
MS.gene91193.t1 AT2G32250 36.452 310 189 2 238 543 310 615 6.16e-64 225
MS.gene91193.t1 AT2G32250 58.163 98 40 1 36 132 41 138 1.60e-28 122
MS.gene91193.t1 AT2G32250 36.452 310 189 2 238 543 269 574 6.57e-64 225
MS.gene91193.t1 AT2G32250 57.732 97 40 1 37 132 1 97 1.15e-27 119
MS.gene91193.t1 AT2G32250 36.452 310 189 2 238 543 310 615 7.72e-64 225
MS.gene91193.t1 AT2G32250 58.163 98 40 1 36 132 41 138 1.60e-28 122
MS.gene91193.t1 AT2G32250 36.452 310 189 2 238 543 310 615 7.72e-64 225
MS.gene91193.t1 AT2G32250 58.163 98 40 1 36 132 41 138 1.60e-28 122
MS.gene91193.t1 AT2G32250 36.452 310 189 2 238 543 310 615 7.72e-64 225
MS.gene91193.t1 AT2G32250 58.163 98 40 1 36 132 41 138 1.60e-28 122
MS.gene91193.t1 AT2G27110 36.213 301 181 6 244 538 198 493 6.87e-57 204
MS.gene91193.t1 AT1G52520 36.513 304 178 6 242 532 391 692 2.79e-56 202
MS.gene91193.t1 AT1G52520 31.019 216 140 4 26 240 78 285 8.79e-21 97.4
MS.gene91193.t1 AT2G27110 36.213 301 181 6 244 538 343 638 2.99e-56 204
MS.gene91193.t1 AT2G27110 27.014 211 128 5 32 240 48 234 8.70e-15 78.6
MS.gene91193.t1 AT2G27110 36.213 301 181 6 244 538 343 638 2.99e-56 204
MS.gene91193.t1 AT2G27110 27.014 211 128 5 32 240 48 234 8.70e-15 78.6
MS.gene91193.t1 AT4G38170 32.063 315 194 7 244 546 161 467 2.78e-53 191
MS.gene91193.t1 AT1G80010 33.446 296 184 6 250 536 423 714 3.53e-46 174
MS.gene91193.t1 AT1G80010 32.500 240 149 4 33 264 95 329 2.40e-23 105
MS.gene91193.t1 AT3G06250 32.394 284 184 4 254 533 479 758 7.54e-42 162
MS.gene91193.t1 AT3G06250 28.571 210 114 4 32 240 189 363 1.65e-18 90.1
MS.gene91193.t1 AT3G06250 32.394 284 184 4 254 533 479 758 7.54e-42 162
MS.gene91193.t1 AT3G06250 28.571 210 114 4 32 240 189 363 1.65e-18 90.1
MS.gene91193.t1 AT3G06250 32.394 284 184 4 254 533 479 758 7.54e-42 162
MS.gene91193.t1 AT3G06250 28.571 210 114 4 32 240 189 363 1.65e-18 90.1
MS.gene91193.t1 AT5G18960 32.653 294 182 4 248 533 497 782 1.95e-41 161
MS.gene91193.t1 AT5G18960 28.774 212 114 5 32 240 210 387 6.74e-15 79.0
MS.gene91193.t1 AT5G18960 32.653 294 182 4 248 533 497 782 1.95e-41 161
MS.gene91193.t1 AT5G18960 28.774 212 114 5 32 240 210 387 6.74e-15 79.0
MS.gene91193.t1 AT5G28530 23.351 561 353 19 33 528 55 603 7.67e-32 131
MS.gene91193.t1 AT5G28530 22.995 561 351 19 33 528 55 599 1.00e-31 130
MS.gene91193.t1 AT1G10240 28.621 290 193 4 237 526 375 650 2.01e-31 130
MS.gene91193.t1 AT1G10240 25.664 226 152 7 25 237 40 262 1.27e-14 77.8
MS.gene91193.t1 AT1G10240 28.621 290 193 4 237 526 375 650 2.01e-31 130
MS.gene91193.t1 AT1G10240 25.664 226 152 7 25 237 40 262 1.27e-14 77.8
MS.gene91193.t1 AT5G28530 25.758 264 187 5 266 528 411 666 6.23e-20 94.7
MS.gene91193.t1 AT5G28530 24.107 224 150 7 33 237 55 277 1.46e-12 71.2
MS.gene91193.t1 AT5G28530 25.000 264 185 5 266 528 411 662 8.26e-20 94.4
MS.gene91193.t1 AT5G28530 24.107 224 150 7 33 237 55 277 1.31e-12 71.2
MS.gene91193.t1 AT5G28530 25.000 264 185 5 266 528 411 662 8.26e-20 94.4
MS.gene91193.t1 AT5G28530 24.107 224 150 7 33 237 55 277 1.31e-12 71.2
MS.gene91193.t1 AT3G07500 38.095 105 61 1 32 132 32 136 3.08e-15 75.5
MS.gene91193.t1 AT3G59470 32.624 141 83 3 22 151 59 198 3.21e-15 76.3
MS.gene91193.t1 AT3G59470 32.624 141 83 3 22 151 59 198 3.21e-15 76.3
MS.gene91193.t1 AT3G59470 32.624 141 83 3 22 151 71 210 3.28e-15 76.6
MS.gene91193.t1 AT2G43280 32.692 104 65 1 32 130 21 124 3.99e-13 69.3
MS.gene91193.t1 AT4G12850 33.636 110 69 2 22 130 3 109 6.18e-13 67.0
MS.gene91193.t1 AT4G12850 34.653 101 65 1 31 130 9 109 6.52e-13 66.6
MS.gene91193.t1 AT4G12850 33.945 109 70 2 31 137 9 117 1.40e-12 67.0

Find 87 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAACAGTTTCATGATAAA+TGG 0.147141 1.3:+12228801 MS.gene91193:CDS
TTGATGGCCTTCCAATTTAT+TGG 0.215906 1.3:+12229358 MS.gene91193:CDS
CATATCATATCTATCCTTAT+TGG 0.228813 1.3:-12229476 None:intergenic
GTTTACATATTTGTCGAAAA+AGG 0.236148 1.3:-12228969 None:intergenic
GAGCTTGATTGATTCTTCTA+TGG 0.262788 1.3:-12228097 None:intergenic
CAGCAAAGATACCAATAAAT+TGG 0.269193 1.3:-12229369 None:intergenic
TGTCGCTTGTTTCAGTATAA+TGG 0.280584 1.3:+12229316 MS.gene91193:CDS
TGAAGATATGTGTTCCAATA+AGG 0.332681 1.3:+12229462 MS.gene91193:CDS
ATCATATGTTGCTGATTTAG+AGG 0.333126 1.3:+12227734 MS.gene91193:CDS
AAGCAGTGGCAAAGTGAAAA+AGG 0.339119 1.3:+12229747 MS.gene91193:CDS
CTTCTCAGTATATAGTTTGA+TGG 0.344346 1.3:-12229394 None:intergenic
TTCCAATGTAAGGTTCTTCA+TGG 0.353929 1.3:-12227764 None:intergenic
AAGTGAAAATAATGATTTCA+TGG 0.355435 1.3:+12228742 MS.gene91193:CDS
AATGATTGCACAATGTTTCA+AGG 0.367492 1.3:-12229584 None:intergenic
AATGTATCTTTCATGTAAAC+AGG 0.380087 1.3:-12228902 None:intergenic
GGAAAATAATGCGCATATGC+TGG 0.400484 1.3:-12228071 None:intergenic
TCAAATTCCATTCCAATGTA+AGG 0.402468 1.3:-12227774 None:intergenic
TTCACTATTGACAGGCTCAT+TGG 0.409194 1.3:-12227712 None:intergenic
TCTTTGTCGACATTCATTGA+TGG 0.410300 1.3:+12229342 MS.gene91193:CDS
TGAAAAGGCCATTCAATTGT+TGG 0.414085 1.3:+12229504 MS.gene91193:CDS
CGTCGTCTGACTTTGGAATC+AGG 0.416108 1.3:+12228257 MS.gene91193:CDS
TCATGATAAATGGGAAGAAA+TGG 0.421780 1.3:+12228811 MS.gene91193:CDS
ATGAAACTGTTGTATTGAAC+GGG 0.423384 1.3:-12228792 None:intergenic
GCCATTCAATTGTTGGAAGA+AGG 0.440928 1.3:+12229511 MS.gene91193:CDS
AAACAAGAAAACTACTTTGA+AGG 0.443130 1.3:+12228988 MS.gene91193:CDS
GTTGAAGTGATGGGACTGTC+TGG 0.444003 1.3:+12229172 MS.gene91193:CDS
TAACTTTGCATGTCAATCAA+TGG 0.444845 1.3:+12229555 MS.gene91193:CDS
TATGCTAAATGTGTCGGATT+TGG 0.446775 1.3:+12227831 MS.gene91193:CDS
ACAACAGTTTCATGATAAAT+GGG 0.448502 1.3:+12228802 MS.gene91193:CDS
AAAAGTAGCAGAGGAAATGT+AGG 0.452921 1.3:+12229619 MS.gene91193:CDS
CATGAAACTGTTGTATTGAA+CGG 0.453502 1.3:-12228793 None:intergenic
GGTGATGGAGTCTTAAGAAC+AGG 0.463539 1.3:-12229094 None:intergenic
TAAGGATCGTCGTCTGACTT+TGG 0.465904 1.3:+12228250 MS.gene91193:CDS
TCCTTCTTCCAACAATTGAA+TGG 0.466774 1.3:-12229512 None:intergenic
CCTTGTTTGAAGGTGGAATG+TGG 0.477763 1.3:+12227966 MS.gene91193:CDS
ATATGATATGTTATATGAAA+AGG 0.478216 1.3:+12229489 MS.gene91193:CDS
AAAATTTCAAGTTGAAGTGA+TGG 0.489947 1.3:+12229162 MS.gene91193:CDS
CAAACTATATACTGAGAAGA+TGG 0.500046 1.3:+12229398 MS.gene91193:CDS
AAGAAGCGTTCGTTGTAGAG+TGG 0.502386 1.3:+12229263 MS.gene91193:CDS
CCTAAACCAGCAAACACAAA+AGG 0.511167 1.3:+12229676 MS.gene91193:CDS
AGGAGATGCAAATGCAATGT+TGG 0.518710 1.3:+12228277 MS.gene91193:CDS
AAATCCACGTCCTTGTTTGA+AGG 0.523815 1.3:+12227956 MS.gene91193:CDS
AAAGAAATTGTGGTGACAGA+TGG 0.532599 1.3:+12228006 MS.gene91193:CDS
AGTGAAAATAATGATTTCAT+GGG 0.538248 1.3:+12228743 MS.gene91193:CDS
TGATTGATTCTTCTATGGCA+AGG 0.542980 1.3:-12228092 None:intergenic
TCCGTGTTGTTTCGCAAGTG+TGG 0.543472 1.3:-12228177 None:intergenic
AGTTGCTACATCAACAAAAG+TGG 0.545982 1.3:+12229938 MS.gene91193:CDS
AAATTTCAAGTTGAAGTGAT+GGG 0.546622 1.3:+12229163 MS.gene91193:CDS
CTTGTTTGAAGGTGGAATGT+GGG 0.548706 1.3:+12227967 MS.gene91193:CDS
AAAATATAAAGTTGCTCTGG+AGG 0.549502 1.3:+12229021 MS.gene91193:CDS
TGAAGAACCTTACATTGGAA+TGG 0.550850 1.3:+12227767 MS.gene91193:CDS
AGAAGCGTTCGTTGTAGAGT+GGG 0.551278 1.3:+12229264 MS.gene91193:CDS
GTATGAGAAAGTTGGTTGCT+CGG 0.554268 1.3:+12228202 MS.gene91193:CDS
AAAGTAGCAGAGGAAATGTA+GGG 0.556137 1.3:+12229620 MS.gene91193:CDS
AAAGATACCAATAAATTGGA+AGG 0.556490 1.3:-12229365 None:intergenic
TCCACGTCCTTGTTTGAAGG+TGG 0.557108 1.3:+12227959 MS.gene91193:CDS
GCTCGATATGCTAAATGTGT+CGG 0.559196 1.3:+12227825 MS.gene91193:CDS
CCACATTCCACCTTCAAACA+AGG 0.563253 1.3:-12227966 None:intergenic
CTGGATGTCATCTTATGCAA+AGG 0.565950 1.3:+12229191 MS.gene91193:CDS
TTACATATCAAAAGAAATTG+TGG 0.567315 1.3:+12227996 MS.gene91193:CDS
GCGATGGATTTGGATGATGA+TGG 0.568217 1.3:+12228344 MS.gene91193:CDS
TGTATGAAGAACGTGAACAT+TGG 0.569682 1.3:+12228876 MS.gene91193:CDS
AAGAAATTGTGGTGACAGAT+GGG 0.571431 1.3:+12228007 MS.gene91193:CDS
AACCATGAAGAACCTTACAT+TGG 0.573124 1.3:+12227762 MS.gene91193:CDS
ACATATGATTCACTATTGAC+AGG 0.579587 1.3:-12227720 None:intergenic
TCCACCTTCAAACAAGGACG+TGG 0.585275 1.3:-12227960 None:intergenic
TGTGATCTTTGATGAAGTCA+TGG 0.586685 1.3:-12228034 None:intergenic
TTCATCATTGATAGTATCAG+AGG 0.587286 1.3:-12229913 None:intergenic
TGCATGTCAATCAATGGAGG+AGG 0.587506 1.3:+12229561 MS.gene91193:CDS
CACGGAGGGTATGAGAAAGT+TGG 0.587975 1.3:+12228194 MS.gene91193:CDS
TTGTTTGAAGGTGGAATGTG+GGG 0.588472 1.3:+12227968 MS.gene91193:CDS
AGAGTGGGATGCATCAAAAG+TGG 0.591450 1.3:+12229279 MS.gene91193:CDS
GCCACACTTGCGAAACAACA+CGG 0.593571 1.3:+12228176 MS.gene91193:CDS
TGGATGTCATCTTATGCAAA+GGG 0.600008 1.3:+12229192 MS.gene91193:CDS
ATCAAGAGTGGGATTGAGAG+AGG 0.606990 1.3:+12229712 MS.gene91193:CDS
TTCAGCTGTCTGATGATGAG+TGG 0.615117 1.3:+12228843 MS.gene91193:CDS
CTCCGAAAAGAATCAAGAGT+GGG 0.620034 1.3:+12229701 MS.gene91193:CDS
ATTGAAACTTTGCAACATGT+TGG 0.622361 1.3:+12228131 MS.gene91193:CDS
GGAAGAAGGATCATTAAGCA+AGG 0.635799 1.3:+12229525 MS.gene91193:CDS
CAATCGATGAAAAGTAGCAG+AGG 0.639675 1.3:+12229610 MS.gene91193:CDS
AAATATGCTTGCACGAGACA+TGG 0.643012 1.3:+12227909 MS.gene91193:CDS
CACTTGCGAAACAACACGGA+GGG 0.644648 1.3:+12228180 MS.gene91193:CDS
CTTTGCATGTCAATCAATGG+AGG 0.647505 1.3:+12229558 MS.gene91193:CDS
GGTCGTAAGAGAACAAGCAG+TGG 0.651877 1.3:+12229733 MS.gene91193:CDS
GCTCCGAAAAGAATCAAGAG+TGG 0.659384 1.3:+12229700 MS.gene91193:CDS
TATACTGAGAAGATGGACAA+AGG 0.676279 1.3:+12229405 MS.gene91193:CDS
ACACTTGCGAAACAACACGG+AGG 0.797516 1.3:+12228179 MS.gene91193:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGATATGTTATATGAAA+AGG + chr1.3:12229489-12229508 MS.gene91193:CDS 15.0%
!!! TATTAGAAATCTTTTAGATA+AGG + chr1.3:12228232-12228251 MS.gene91193:CDS 15.0%
!! AAGTGAAAATAATGATTTCA+TGG + chr1.3:12228742-12228761 MS.gene91193:CDS 20.0%
!! AGTGAAAATAATGATTTCAT+GGG + chr1.3:12228743-12228762 MS.gene91193:CDS 20.0%
!! TTACATATCAAAAGAAATTG+TGG + chr1.3:12227996-12228015 MS.gene91193:CDS 20.0%
!!! ACAATTTCTTTTGATATGTA+AGG - chr1.3:12227997-12228016 None:intergenic 20.0%
!!! TACATGAAAGATACATTTTT+TGG + chr1.3:12228908-12228927 MS.gene91193:CDS 20.0%
! AAACAAGAAAACTACTTTGA+AGG + chr1.3:12228988-12229007 MS.gene91193:CDS 25.0%
! AAAGATACCAATAAATTGGA+AGG - chr1.3:12229368-12229387 None:intergenic 25.0%
! AAATGAGAGAGATAATTAGT+TGG - chr1.3:12230021-12230040 None:intergenic 25.0%
! AATGTATCTTTCATGTAAAC+AGG - chr1.3:12228905-12228924 None:intergenic 25.0%
! ACAACAGTTTCATGATAAAT+GGG + chr1.3:12228802-12228821 MS.gene91193:CDS 25.0%
! ATAGATGATTACAAAGAGTT+TGG + chr1.3:12228404-12228423 MS.gene91193:intron 25.0%
! CATATCATATCTATCCTTAT+TGG - chr1.3:12229479-12229498 None:intergenic 25.0%
! GTTTACATATTTGTCGAAAA+AGG - chr1.3:12228972-12228991 None:intergenic 25.0%
! TACAACAGTTTCATGATAAA+TGG + chr1.3:12228801-12228820 MS.gene91193:CDS 25.0%
! TGAAAAATATAAAGTTGCTC+TGG + chr1.3:12229018-12229037 MS.gene91193:CDS 25.0%
!! TCGTCTGAAAAATGTATTTT+GGG + chr1.3:12228367-12228386 MS.gene91193:CDS 25.0%
!!! AAAATTTCAAGTTGAAGTGA+TGG + chr1.3:12229162-12229181 MS.gene91193:CDS 25.0%
!!! AAATTTCAAGTTGAAGTGAT+GGG + chr1.3:12229163-12229182 MS.gene91193:CDS 25.0%
!!! ATGAAAGATACATTTTTTGG+TGG + chr1.3:12228911-12228930 MS.gene91193:CDS 25.0%
!!! ATTGACATTTGCTTTTCAAA+AGG - chr1.3:12229118-12229137 None:intergenic 25.0%
!!! TTTTGAAAAGCAAATGTCAA+TGG + chr1.3:12229117-12229136 MS.gene91193:CDS 25.0%
AAAATATAAAGTTGCTCTGG+AGG + chr1.3:12229021-12229040 MS.gene91193:CDS 30.0%
AATGATTGCACAATGTTTCA+AGG - chr1.3:12229587-12229606 None:intergenic 30.0%
ACATATGATTCACTATTGAC+AGG - chr1.3:12227723-12227742 None:intergenic 30.0%
ATGAAACTGTTGTATTGAAC+GGG - chr1.3:12228795-12228814 None:intergenic 30.0%
ATTGAAACTTTGCAACATGT+TGG + chr1.3:12228131-12228150 MS.gene91193:CDS 30.0%
CAAACTATATACTGAGAAGA+TGG + chr1.3:12229398-12229417 MS.gene91193:CDS 30.0%
CAGCAAAGATACCAATAAAT+TGG - chr1.3:12229372-12229391 None:intergenic 30.0%
CATGAAACTGTTGTATTGAA+CGG - chr1.3:12228796-12228815 None:intergenic 30.0%
CTTCTCAGTATATAGTTTGA+TGG - chr1.3:12229397-12229416 None:intergenic 30.0%
TAACTTTGCATGTCAATCAA+TGG + chr1.3:12229555-12229574 MS.gene91193:CDS 30.0%
TCAAATTCCATTCCAATGTA+AGG - chr1.3:12227777-12227796 None:intergenic 30.0%
TCAGTTATTATTGCATTTGG+AGG - chr1.3:12228611-12228630 None:intergenic 30.0%
TCATGATAAATGGGAAGAAA+TGG + chr1.3:12228811-12228830 MS.gene91193:CDS 30.0%
TGAAGATATGTGTTCCAATA+AGG + chr1.3:12229462-12229481 MS.gene91193:CDS 30.0%
TGATTATTCACACCAATGAA+GGG - chr1.3:12228488-12228507 None:intergenic 30.0%
TGGTCAGTTATTATTGCATT+TGG - chr1.3:12228614-12228633 None:intergenic 30.0%
! CTTCATTCGTCTTTCTTTTA+TGG - chr1.3:12229447-12229466 None:intergenic 30.0%
! CTTTTCTCAATTTTCAGTCA+AGG + chr1.3:12230042-12230061 MS.gene91193:intron 30.0%
! GTCGTCTGAAAAATGTATTT+TGG + chr1.3:12228366-12228385 MS.gene91193:CDS 30.0%
! GTTTAGAAATTTTCGAACGA+CGG - chr1.3:12227875-12227894 None:intergenic 30.0%
! TTCAAGATTCTTTTATGCGA+TGG + chr1.3:12228328-12228347 MS.gene91193:CDS 30.0%
! TTCATCATTGATAGTATCAG+AGG - chr1.3:12229916-12229935 None:intergenic 30.0%
!! AGCAAGCTGATTTTAATACA+TGG + chr1.3:12229062-12229081 MS.gene91193:CDS 30.0%
!! ATCATATGTTGCTGATTTAG+AGG + chr1.3:12227734-12227753 MS.gene91193:CDS 30.0%
!! ATTCTTTTATGCGATGGATT+TGG + chr1.3:12228334-12228353 MS.gene91193:CDS 30.0%
!! ATTTGCTTTTCAAAAGGTGA+TGG - chr1.3:12229112-12229131 None:intergenic 30.0%
!! TCTGAAAAATGTATTTTGGG+TGG + chr1.3:12228370-12228389 MS.gene91193:CDS 30.0%
!!! TCATTGTTGTTTTTGTGATG+TGG + chr1.3:12229843-12229862 MS.gene91193:intron 30.0%
AAAAGTAGCAGAGGAAATGT+AGG + chr1.3:12229619-12229638 MS.gene91193:CDS 35.0%
AAAGAAATTGTGGTGACAGA+TGG + chr1.3:12228006-12228025 MS.gene91193:CDS 35.0%
AAAGTAGCAGAGGAAATGTA+GGG + chr1.3:12229620-12229639 MS.gene91193:CDS 35.0%
AACCATGAAGAACCTTACAT+TGG + chr1.3:12227762-12227781 MS.gene91193:CDS 35.0%
AAGAAATTGTGGTGACAGAT+GGG + chr1.3:12228007-12228026 MS.gene91193:CDS 35.0%
AATGAAAGTAGCTATTGGAC+AGG + chr1.3:12228643-12228662 MS.gene91193:intron 35.0%
AGAGCAATGAAAGTAGCTAT+TGG + chr1.3:12228638-12228657 MS.gene91193:intron 35.0%
AGTTGCTACATCAACAAAAG+TGG + chr1.3:12229938-12229957 MS.gene91193:CDS 35.0%
ATTACTAGCAAATGAGACCT+CGG + chr1.3:12228535-12228554 MS.gene91193:intron 35.0%
CACTTTCAATCAAGATTGCT+TGG + chr1.3:12228506-12228525 MS.gene91193:intron 35.0%
GTGATTATTCACACCAATGA+AGG - chr1.3:12228489-12228508 None:intergenic 35.0%
TATACTGAGAAGATGGACAA+AGG + chr1.3:12229405-12229424 MS.gene91193:CDS 35.0%
TATGCTAAATGTGTCGGATT+TGG + chr1.3:12227831-12227850 MS.gene91193:CDS 35.0%
TCCTTCTTCCAACAATTGAA+TGG - chr1.3:12229515-12229534 None:intergenic 35.0%
TCTTTGTCGACATTCATTGA+TGG + chr1.3:12229342-12229361 MS.gene91193:CDS 35.0%
TGAAAAGGCCATTCAATTGT+TGG + chr1.3:12229504-12229523 MS.gene91193:CDS 35.0%
TGAAGAACCTTACATTGGAA+TGG + chr1.3:12227767-12227786 MS.gene91193:CDS 35.0%
TGGATGTCATCTTATGCAAA+GGG + chr1.3:12229192-12229211 MS.gene91193:CDS 35.0%
TGGTAAAAAACGAGAATCGA+CGG + chr1.3:12227929-12227948 MS.gene91193:CDS 35.0%
TGTATGAAGAACGTGAACAT+TGG + chr1.3:12228876-12228895 MS.gene91193:CDS 35.0%
TGTGATCTTTGATGAAGTCA+TGG - chr1.3:12228037-12228056 None:intergenic 35.0%
TTCCAATGTAAGGTTCTTCA+TGG - chr1.3:12227767-12227786 None:intergenic 35.0%
! AGTGTGGCAAAAATTTTGCT+TGG - chr1.3:12228164-12228183 None:intergenic 35.0%
! ATAACATGACCGAGTTTTTC+AGG - chr1.3:12228722-12228741 None:intergenic 35.0%
! ATCCCACTCTTGATTCTTTT+CGG - chr1.3:12229706-12229725 None:intergenic 35.0%
! CACTCTTCATTTTCATCGAT+AGG - chr1.3:12227687-12227706 None:intergenic 35.0%
! GAGCTTGATTGATTCTTCTA+TGG - chr1.3:12228100-12228119 None:intergenic 35.0%
! GTTGATGAAAACTTGGCTTA+GGG + chr1.3:12228571-12228590 MS.gene91193:intron 35.0%
! TGATTGATTCTTCTATGGCA+AGG - chr1.3:12228095-12228114 None:intergenic 35.0%
! TGTCGCTTGTTTCAGTATAA+TGG + chr1.3:12229316-12229335 MS.gene91193:CDS 35.0%
! TTGATGGCCTTCCAATTTAT+TGG + chr1.3:12229358-12229377 MS.gene91193:CDS 35.0%
!! AGAAAAGTGCCTGAAAAACT+CGG + chr1.3:12228710-12228729 MS.gene91193:intron 35.0%
!! TCACATGGTTGATGAAAACT+TGG + chr1.3:12228564-12228583 MS.gene91193:intron 35.0%
AAAACTTGGCTTAGGGCAAT+GGG + chr1.3:12228578-12228597 MS.gene91193:intron 40.0%
AAATATGCTTGCACGAGACA+TGG + chr1.3:12227909-12227928 MS.gene91193:CDS 40.0%
AAGCAGTGGCAAAGTGAAAA+AGG + chr1.3:12229747-12229766 MS.gene91193:CDS 40.0%
AGGAGATGCAAATGCAATGT+TGG + chr1.3:12228277-12228296 MS.gene91193:CDS 40.0%
CAAAAACGATGTCGAGCATT+TGG - chr1.3:12228674-12228693 None:intergenic 40.0%
CAATCGATGAAAAGTAGCAG+AGG + chr1.3:12229610-12229629 MS.gene91193:CDS 40.0%
CCTAAACCAGCAAACACAAA+AGG + chr1.3:12229676-12229695 MS.gene91193:CDS 40.0%
CTACTTTCATTGCTCTGTCT+TGG - chr1.3:12228634-12228653 None:intergenic 40.0%
CTCCGAAAAGAATCAAGAGT+GGG + chr1.3:12229701-12229720 MS.gene91193:CDS 40.0%
CTGGATGTCATCTTATGCAA+AGG + chr1.3:12229191-12229210 MS.gene91193:CDS 40.0%
CTTGTTTGAAGGTGGAATGT+GGG + chr1.3:12227967-12227986 MS.gene91193:CDS 40.0%
CTTTGCATGTCAATCAATGG+AGG + chr1.3:12229558-12229577 MS.gene91193:CDS 40.0%
GCCATTCAATTGTTGGAAGA+AGG + chr1.3:12229511-12229530 MS.gene91193:CDS 40.0%
GCTCGATATGCTAAATGTGT+CGG + chr1.3:12227825-12227844 MS.gene91193:CDS 40.0%
GGAAAATAATGCGCATATGC+TGG - chr1.3:12228074-12228093 None:intergenic 40.0%
GGAAGAAGGATCATTAAGCA+AGG + chr1.3:12229525-12229544 MS.gene91193:CDS 40.0%
TTCACTATTGACAGGCTCAT+TGG - chr1.3:12227715-12227734 None:intergenic 40.0%
TTGTTTGAAGGTGGAATGTG+GGG + chr1.3:12227968-12227987 MS.gene91193:CDS 40.0%
! AAATCCACGTCCTTGTTTGA+AGG + chr1.3:12227956-12227975 MS.gene91193:CDS 40.0%
! GTATGAGAAAGTTGGTTGCT+CGG + chr1.3:12228202-12228221 MS.gene91193:CDS 40.0%
!! CTCGACATCGTTTTTGTCTT+TGG + chr1.3:12228678-12228697 MS.gene91193:intron 40.0%
!! GGTTGATGAAAACTTGGCTT+AGG + chr1.3:12228570-12228589 MS.gene91193:intron 40.0%
!! GTATTTTGGGTGGATGCAAA+TGG + chr1.3:12228380-12228399 MS.gene91193:CDS 40.0%
!!! CCTTTTGTGTTTGCTGGTTT+AGG - chr1.3:12229679-12229698 None:intergenic 40.0%
ACACCAATGAAGGGAGCAAA+TGG - chr1.3:12228479-12228498 None:intergenic 45.0%
AGAGTGGGATGCATCAAAAG+TGG + chr1.3:12229279-12229298 MS.gene91193:CDS 45.0%
ATCAAGAGTGGGATTGAGAG+AGG + chr1.3:12229712-12229731 MS.gene91193:CDS 45.0%
CCACATTCCACCTTCAAACA+AGG - chr1.3:12227969-12227988 None:intergenic 45.0%
GAAAACTTGGCTTAGGGCAA+TGG + chr1.3:12228577-12228596 MS.gene91193:intron 45.0%
GCTCCGAAAAGAATCAAGAG+TGG + chr1.3:12229700-12229719 MS.gene91193:CDS 45.0%
TAAGGATCGTCGTCTGACTT+TGG + chr1.3:12228250-12228269 MS.gene91193:CDS 45.0%
TGCATGTCAATCAATGGAGG+AGG + chr1.3:12229561-12229580 MS.gene91193:CDS 45.0%
! ATGCCATTTGCTCCCTTCAT+TGG + chr1.3:12228473-12228492 MS.gene91193:intron 45.0%
! CCTTGTTTGAAGGTGGAATG+TGG + chr1.3:12227966-12227985 MS.gene91193:CDS 45.0%
! GCGATGGATTTGGATGATGA+TGG + chr1.3:12228344-12228363 MS.gene91193:CDS 45.0%
! TTCAGCTGTCTGATGATGAG+TGG + chr1.3:12228843-12228862 MS.gene91193:CDS 45.0%
!! AAGAAGCGTTCGTTGTAGAG+TGG + chr1.3:12229263-12229282 MS.gene91193:CDS 45.0%
!! AGAAGCGTTCGTTGTAGAGT+GGG + chr1.3:12229264-12229283 MS.gene91193:CDS 45.0%
!! GGTGATGGAGTCTTAAGAAC+AGG - chr1.3:12229097-12229116 None:intergenic 45.0%
ACACTTGCGAAACAACACGG+AGG + chr1.3:12228179-12228198 MS.gene91193:CDS 50.0%
AGACCTCGGAGACTTTCACA+TGG + chr1.3:12228549-12228568 MS.gene91193:intron 50.0%
CAACCATGTGAAAGTCTCCG+AGG - chr1.3:12228555-12228574 None:intergenic 50.0%
CACTTGCGAAACAACACGGA+GGG + chr1.3:12228180-12228199 MS.gene91193:CDS 50.0%
GCCACACTTGCGAAACAACA+CGG + chr1.3:12228176-12228195 MS.gene91193:CDS 50.0%
GGTCGTAAGAGAACAAGCAG+TGG + chr1.3:12229733-12229752 MS.gene91193:CDS 50.0%
TCCACCTTCAAACAAGGACG+TGG - chr1.3:12227963-12227982 None:intergenic 50.0%
! CACGGAGGGTATGAGAAAGT+TGG + chr1.3:12228194-12228213 MS.gene91193:CDS 50.0%
! GGAGCTCCTTTTGTGTTTGC+TGG - chr1.3:12229685-12229704 None:intergenic 50.0%
! GTTGAAGTGATGGGACTGTC+TGG + chr1.3:12229172-12229191 MS.gene91193:CDS 50.0%
! TCCACGTCCTTGTTTGAAGG+TGG + chr1.3:12227959-12227978 MS.gene91193:CDS 50.0%
!! CGTCGTCTGACTTTGGAATC+AGG + chr1.3:12228257-12228276 MS.gene91193:CDS 50.0%
!! TCCGTGTTGTTTCGCAAGTG+TGG - chr1.3:12228180-12228199 None:intergenic 50.0%
ACTTGGCTTAGGGCAATGGG+TGG + chr1.3:12228581-12228600 MS.gene91193:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 12227678 12230070 12227678 ID=MS.gene91193
chr1.3 mRNA 12227678 12230070 12227678 ID=MS.gene91193.t1;Parent=MS.gene91193
chr1.3 exon 12227678 12228401 12227678 ID=MS.gene91193.t1.exon1;Parent=MS.gene91193.t1
chr1.3 CDS 12227678 12228401 12227678 ID=cds.MS.gene91193.t1;Parent=MS.gene91193.t1
chr1.3 exon 12228717 12229768 12228717 ID=MS.gene91193.t1.exon2;Parent=MS.gene91193.t1
chr1.3 CDS 12228717 12229768 12228717 ID=cds.MS.gene91193.t1;Parent=MS.gene91193.t1
chr1.3 exon 12229882 12229959 12229882 ID=MS.gene91193.t1.exon3;Parent=MS.gene91193.t1
chr1.3 CDS 12229882 12229959 12229882 ID=cds.MS.gene91193.t1;Parent=MS.gene91193.t1
chr1.3 exon 12230059 12230070 12230059 ID=MS.gene91193.t1.exon4;Parent=MS.gene91193.t1
chr1.3 CDS 12230059 12230070 12230059 ID=cds.MS.gene91193.t1;Parent=MS.gene91193.t1
Gene Sequence

>MS.gene91193

ATGGAACCTATCGATGAAAATGAAGAGTGTGTTGCCAATGAGCCTGTCAATAGTGAATCATATGTTGCTGATTTAGAGGTTAAGAACCATGAAGAACCTTACATTGGAATGGAATTTGAGTCACAAGAAGATGCTTATTCGTTCTATGCTCGATATGCTAAATGTGTCGGATTTGGTGTATGCACTAAAAGTAGCCGTCGTTCGAAAATTTCTAAACAATTTATTGATGTGAAATATGCTTGCACGAGACATGGTAAAAAACGAGAATCGACGGCACAAAATCCACGTCCTTGTTTGAAGGTGGAATGTGGGGCTGCCTTACATATCAAAAGAAATTGTGGTGACAGATGGGTGATCCATGACTTCATCAAAGATCACAATCATGATCTTTTTCCAGCATATGCGCATTATTTTCCTTGCCATAGAAGAATCAATCAAGCTCAAAAGCAATGTATTGAAACTTTGCAACATGTTGGTGTGAGACCAAGCAAAATTTTTGCCACACTTGCGAAACAACACGGAGGGTATGAGAAAGTTGGTTGCTCGGAGAAAGATATTAGAAATCTTTTAGATAAGGATCGTCGTCTGACTTTGGAATCAGGAGATGCAAATGCAATGTTGGAGTGTTTTACGCTTATGCAAGAACGAAATTCAAGATTCTTTTATGCGATGGATTTGGATGATGATGGTCGTCTGAAAAATGTATTTTGGGTGGATGCAAATGGTATAGATGATTACAAAGAGTTTGGAGATGTGATATCTTTTGATACAACATATATCACAAACAAATATAAAATGCCATTTGCTCCCTTCATTGGTGTGAATAATCACTTTCAATCAAGATTGCTTGGTTGTGCATTACTAGCAAATGAGACCTCGGAGACTTTCACATGGTTGATGAAAACTTGGCTTAGGGCAATGGGTGGCAAGCCTCCAAATGCAATAATAACTGACCAAGACAGAGCAATGAAAGTAGCTATTGGACAGGTATTTCCAAATGCTCGACATCGTTTTTGTCTTTGGCATATACTTAGAAAAGTGCCTGAAAAACTCGGTCATGTTATAAGTGAAAATAATGATTTCATGGGATATTTTAACTCGTGCATTTACAAGTCCCGTTCAATACAACAGTTTCATGATAAATGGGAAGAAATGGTAGAAAAATTTCAGCTGTCTGATGATGAGTGGATTCAGTCGTTGTATGAAGAACGTGAACATTGGATTCCTGTTTACATGAAAGATACATTTTTTGGTGGTATGTCAACAACTCAAAGATCAGAGAGCATCAACTCCTTTTTCGACAAATATGTAAACAAGAAAACTACTTTGAAGGAATTTGTTGAAAAATATAAAGTTGCTCTGGAGGATAGAGAAGAAGCAGAGAAGCAAGCTGATTTTAATACATGGCACAAGCAACCTGTTCTTAAGACTCCATCACCTTTTGAAAAGCAAATGTCAATGGTTTATACACATGAAGTGTTCAAAAAATTTCAAGTTGAAGTGATGGGACTGTCTGGATGTCATCTTATGCAAAGGGATGAAGATAATCATCTCACTTCTTTTACAATATACGACTTTGAGAAGAAAGAAGCGTTCGTTGTAGAGTGGGATGCATCAAAAGTGGAAATTTCTTGTATATGTCGCTTGTTTCAGTATAATGGTTATCTTTGTCGACATTCATTGATGGCCTTCCAATTTATTGGTATCTTTGCTGTTCCATCAAACTATATACTGAGAAGATGGACAAAGGATATTAGAAGTTTGCACCATAAAAGAAAGACGAATGAAGATATGTGTTCCAATAAGGATAGATATGATATGTTATATGAAAAGGCCATTCAATTGTTGGAAGAAGGATCATTAAGCAAGGAGAGTTGTAACTTTGCATGTCAATCAATGGAGGAGGCCTTGAAACATTGTGCAATCATTAATCAATCGATGAAAAGTAGCAGAGGAAATGTAGGGAAGAAAATTTTACTTGACAAGAAATTACTTGATCCTAAACCAGCAAACACAAAAGGAGCTCCGAAAAGAATCAAGAGTGGGATTGAGAGAGGTCGTAAGAGAACAAGCAGTGGCAAAGTGAAAAAGGTTAGAATTTATTGCTTTGCTGTGATTTTCATTTCACTAAACTTTCTTAAAATATTCATATTTAAGATGATTTCTCATTGTTGTTTTTGTGATGTGGTTATGTGAAAATGAAGTCTGCATTTGTTGAAAAAGAAGCAGTGATATCCTCTGATACTATCAATGATGAAGCAGTTGCTACATCAACAAAAGTGGTATGAGATTTCAATATTGAACTTGATGCATGTTTATTCTAGAGCTGCATTTGAGTAACCAACTAATTATCTCTCTCATTTTCTTTTCTCAATTTTCAGTCAAGGAAGTAG
Protein sequence

>MS.gene91193.t1

MEPIDENEECVANEPVNSESYVADLEVKNHEEPYIGMEFESQEDAYSFYARYAKCVGFGVCTKSSRRSKISKQFIDVKYACTRHGKKRESTAQNPRPCLKVECGAALHIKRNCGDRWVIHDFIKDHNHDLFPAYAHYFPCHRRINQAQKQCIETLQHVGVRPSKIFATLAKQHGGYEKVGCSEKDIRNLLDKDRRLTLESGDANAMLECFTLMQERNSRFFYAMDLDDDGRLKNVFWVDANVPEKLGHVISENNDFMGYFNSCIYKSRSIQQFHDKWEEMVEKFQLSDDEWIQSLYEEREHWIPVYMKDTFFGGMSTTQRSESINSFFDKYVNKKTTLKEFVEKYKVALEDREEAEKQADFNTWHKQPVLKTPSPFEKQMSMVYTHEVFKKFQVEVMGLSGCHLMQRDEDNHLTSFTIYDFEKKEAFVVEWDASKVEISCICRLFQYNGYLCRHSLMAFQFIGIFAVPSNYILRRWTKDIRSLHHKRKTNEDMCSNKDRYDMLYEKAIQLLEEGSLSKESCNFACQSMEEALKHCAIINQSMKSSRGNVGKKILLDKKLLDPKPANTKGAPKRIKSGIERGRKRTSSGKVKKSAFVEKEAVISSDTINDEAVATSTKVSRK