Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91286.t1 | NP_001235999.1 | 93.9 | 49 | 3 | 0 | 13 | 61 | 80 | 128 | 1.50E-19 | 104.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91286.t1 | Q2V9B0 | 87.8 | 49 | 6 | 0 | 13 | 61 | 95 | 143 | 3.0e-21 | 101.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91286.t1 | A0A445L1K2 | 93.9 | 49 | 3 | 0 | 13 | 61 | 80 | 128 | 1.1e-19 | 104.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene91286.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91286.t1 | MTR_4g107230 | 93.617 | 47 | 3 | 0 | 13 | 59 | 84 | 130 | 6.13e-29 | 103 |
| MS.gene91286.t1 | MTR_8g101650 | 73.333 | 60 | 15 | 1 | 3 | 61 | 94 | 153 | 2.27e-28 | 102 |
| MS.gene91286.t1 | MTR_2g090305 | 87.500 | 48 | 6 | 0 | 13 | 60 | 28 | 75 | 6.64e-28 | 98.6 |
| MS.gene91286.t1 | MTR_6g092540 | 85.714 | 49 | 7 | 0 | 13 | 61 | 97 | 145 | 8.58e-28 | 100 |
| MS.gene91286.t1 | MTR_0036s0260 | 85.417 | 48 | 7 | 0 | 13 | 60 | 28 | 75 | 4.76e-27 | 97.1 |
| MS.gene91286.t1 | MTR_5g037080 | 85.417 | 48 | 7 | 0 | 13 | 60 | 102 | 149 | 2.15e-26 | 97.4 |
| MS.gene91286.t1 | MTR_2g100930 | 77.551 | 49 | 11 | 0 | 13 | 61 | 109 | 157 | 1.46e-24 | 93.2 |
| MS.gene91286.t1 | MTR_4g100630 | 79.592 | 49 | 10 | 0 | 13 | 61 | 95 | 143 | 2.04e-24 | 92.4 |
| MS.gene91286.t1 | MTR_4g111975 | 75.000 | 52 | 13 | 0 | 10 | 61 | 135 | 186 | 6.64e-24 | 90.9 |
| MS.gene91286.t1 | MTR_3g462790 | 79.592 | 49 | 10 | 0 | 13 | 61 | 147 | 195 | 7.66e-24 | 90.9 |
| MS.gene91286.t1 | MTR_5g069710 | 79.592 | 49 | 10 | 0 | 13 | 61 | 132 | 180 | 1.07e-23 | 90.1 |
| MS.gene91286.t1 | MTR_7g067080 | 77.551 | 49 | 11 | 0 | 13 | 61 | 140 | 188 | 2.09e-23 | 89.7 |
| MS.gene91286.t1 | MTR_1g111830 | 81.250 | 48 | 9 | 0 | 14 | 61 | 125 | 172 | 7.63e-23 | 87.0 |
| MS.gene91286.t1 | MTR_3g104370 | 75.510 | 49 | 12 | 0 | 13 | 61 | 91 | 139 | 1.04e-22 | 86.3 |
| MS.gene91286.t1 | MTR_5g081860 | 73.469 | 49 | 13 | 0 | 13 | 61 | 125 | 173 | 1.61e-21 | 84.0 |
| MS.gene91286.t1 | MTR_5g088010 | 58.491 | 53 | 22 | 0 | 8 | 60 | 55 | 107 | 3.32e-18 | 74.7 |
| MS.gene91286.t1 | MTR_8g063600 | 67.308 | 52 | 17 | 0 | 10 | 61 | 103 | 154 | 4.06e-18 | 75.1 |
| MS.gene91286.t1 | MTR_5g027550 | 62.500 | 48 | 18 | 0 | 13 | 60 | 77 | 124 | 2.21e-17 | 72.4 |
| MS.gene91286.t1 | MTR_5g027570 | 58.824 | 51 | 21 | 0 | 10 | 60 | 89 | 139 | 5.10e-17 | 71.6 |
| MS.gene91286.t1 | MTR_1g048660 | 65.217 | 46 | 16 | 0 | 14 | 59 | 13 | 58 | 5.10e-17 | 68.2 |
| MS.gene91286.t1 | MTR_5g088060 | 65.217 | 46 | 16 | 0 | 15 | 60 | 274 | 319 | 1.57e-16 | 71.6 |
| MS.gene91286.t1 | MTR_5g488160 | 65.217 | 46 | 16 | 0 | 15 | 60 | 274 | 319 | 1.57e-16 | 71.6 |
| MS.gene91286.t1 | MTR_8g063870 | 66.667 | 45 | 15 | 0 | 15 | 59 | 94 | 138 | 2.09e-16 | 70.5 |
| MS.gene91286.t1 | MTR_4g015130 | 69.231 | 39 | 12 | 0 | 23 | 61 | 3 | 41 | 4.21e-15 | 63.2 |
| MS.gene91286.t1 | MTR_1g033620 | 79.412 | 34 | 7 | 0 | 28 | 61 | 20 | 53 | 2.03e-13 | 62.0 |
| MS.gene91286.t1 | MTR_5g075760 | 63.043 | 46 | 16 | 1 | 15 | 60 | 107 | 151 | 5.85e-13 | 60.5 |
| MS.gene91286.t1 | MTR_5g075790 | 60.870 | 46 | 17 | 1 | 15 | 60 | 115 | 159 | 1.73e-12 | 59.3 |
| MS.gene91286.t1 | MTR_1g083180 | 57.447 | 47 | 19 | 1 | 15 | 61 | 63 | 108 | 2.18e-12 | 58.5 |
| MS.gene91286.t1 | MTR_3g070110 | 56.818 | 44 | 19 | 0 | 15 | 58 | 97 | 140 | 5.37e-12 | 57.8 |
| MS.gene91286.t1 | MTR_8g072990 | 42.593 | 54 | 31 | 0 | 6 | 59 | 93 | 146 | 1.30e-11 | 57.0 |
| MS.gene91286.t1 | MTR_6g043490 | 40.741 | 54 | 32 | 0 | 6 | 59 | 100 | 153 | 4.40e-11 | 55.5 |
| MS.gene91286.t1 | MTR_6g043480 | 40.741 | 54 | 32 | 0 | 6 | 59 | 100 | 153 | 9.02e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91286.t1 | AT1G70000 | 87.234 | 47 | 6 | 0 | 14 | 60 | 99 | 145 | 4.99e-27 | 98.2 |
| MS.gene91286.t1 | AT1G70000 | 87.234 | 47 | 6 | 0 | 14 | 60 | 99 | 145 | 4.99e-27 | 98.2 |
| MS.gene91286.t1 | AT1G19000 | 80.851 | 47 | 9 | 0 | 13 | 59 | 102 | 148 | 8.71e-26 | 95.5 |
| MS.gene91286.t1 | AT1G19000 | 80.851 | 47 | 9 | 0 | 13 | 59 | 102 | 148 | 8.71e-26 | 95.5 |
| MS.gene91286.t1 | AT5G47390 | 85.417 | 48 | 7 | 0 | 14 | 61 | 97 | 144 | 1.67e-25 | 95.9 |
| MS.gene91286.t1 | AT1G74840 | 80.851 | 47 | 9 | 0 | 13 | 59 | 96 | 142 | 4.27e-25 | 92.8 |
| MS.gene91286.t1 | AT3G16350 | 81.250 | 48 | 9 | 0 | 13 | 60 | 137 | 184 | 6.58e-25 | 94.7 |
| MS.gene91286.t1 | AT1G74840 | 80.851 | 47 | 9 | 0 | 13 | 59 | 96 | 142 | 8.70e-25 | 92.4 |
| MS.gene91286.t1 | AT5G58900 | 79.592 | 49 | 10 | 0 | 13 | 61 | 141 | 189 | 1.85e-24 | 92.0 |
| MS.gene91286.t1 | AT5G56840 | 77.551 | 49 | 11 | 0 | 13 | 61 | 91 | 139 | 1.89e-24 | 90.9 |
| MS.gene91286.t1 | AT1G49010 | 75.000 | 52 | 13 | 0 | 10 | 61 | 131 | 182 | 6.89e-24 | 90.9 |
| MS.gene91286.t1 | AT2G38090 | 75.510 | 49 | 12 | 0 | 13 | 61 | 141 | 189 | 4.65e-23 | 88.6 |
| MS.gene91286.t1 | AT5G08520 | 73.077 | 52 | 14 | 0 | 10 | 61 | 115 | 166 | 6.24e-23 | 88.2 |
| MS.gene91286.t1 | AT5G04760 | 70.175 | 57 | 17 | 0 | 5 | 61 | 91 | 147 | 7.11e-23 | 86.3 |
| MS.gene91286.t1 | AT5G61620 | 75.000 | 48 | 12 | 0 | 14 | 61 | 109 | 156 | 9.78e-23 | 87.8 |
| MS.gene91286.t1 | AT5G05790 | 69.231 | 52 | 16 | 0 | 10 | 61 | 128 | 179 | 3.16e-21 | 83.2 |
| MS.gene91286.t1 | AT5G05790 | 69.231 | 52 | 16 | 0 | 10 | 61 | 128 | 179 | 3.16e-21 | 83.2 |
| MS.gene91286.t1 | AT5G01200 | 71.429 | 49 | 14 | 0 | 13 | 61 | 146 | 194 | 5.73e-21 | 82.4 |
| MS.gene91286.t1 | AT3G11280 | 71.429 | 49 | 14 | 0 | 13 | 61 | 127 | 175 | 1.05e-20 | 81.6 |
| MS.gene91286.t1 | AT3G11280 | 71.429 | 49 | 14 | 0 | 13 | 61 | 127 | 175 | 1.05e-20 | 81.6 |
| MS.gene91286.t1 | AT3G10580 | 67.347 | 49 | 16 | 0 | 13 | 61 | 95 | 143 | 5.15e-19 | 77.0 |
| MS.gene91286.t1 | AT3G10580 | 67.347 | 49 | 16 | 0 | 13 | 61 | 95 | 143 | 5.31e-19 | 77.4 |
| MS.gene91286.t1 | AT4G09450 | 65.385 | 52 | 18 | 0 | 10 | 61 | 88 | 139 | 9.74e-19 | 75.5 |
| MS.gene91286.t1 | AT5G23650 | 66.000 | 50 | 17 | 0 | 10 | 59 | 117 | 166 | 4.38e-16 | 70.1 |
| MS.gene91286.t1 | AT3G10590 | 60.000 | 45 | 18 | 0 | 14 | 58 | 112 | 156 | 9.36e-13 | 59.7 |
Find 15 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATGTGGCTTACATGATC+GGG | 0.431194 | 1.3:+73224753 | None:intergenic |
| TTGGTTGGATTGGAGAAAGT+GGG | 0.463944 | 1.3:-73223958 | MS.gene91286:CDS |
| GGGAATGTGGCTTACATGAT+CGG | 0.480428 | 1.3:+73224752 | None:intergenic |
| AGAAAGTGGGAAAAGGTGAT+TGG | 0.482958 | 1.3:-73223945 | MS.gene91286:CDS |
| TTTGGTTGGATTGGAGAAAG+TGG | 0.531455 | 1.3:-73223959 | MS.gene91286:CDS |
| GGATTGGAGAAAGTGGGAAA+AGG | 0.562648 | 1.3:-73223952 | MS.gene91286:CDS |
| TTGTGCTCTTCCTCAGTCCA+CGG | 0.578432 | 1.3:+73223988 | None:intergenic |
| GTGATTGGAGAGGAATCTCA+AGG | 0.619264 | 1.3:-73223930 | MS.gene91286:CDS |
| GTGGGAAAAGGTGATTGGAG+AGG | 0.624127 | 1.3:-73223940 | MS.gene91286:CDS |
| TGAATGGATCAGGGATTCCG+TGG | 0.626840 | 1.3:-73224005 | MS.gene91286:intron |
| TGACATGGAATGGTGTTGCT+CGG | 0.634157 | 1.3:-73224787 | None:intergenic |
| GCATGACTTGCAACATGCGT+AGG | 0.636946 | 1.3:+73223886 | None:intergenic |
| ATCAGGGATTCCGTGGACTG+AGG | 0.660333 | 1.3:-73223998 | MS.gene91286:intron |
| GAATGTGGCTTACATGATCG+GGG | 0.660918 | 1.3:+73224754 | None:intergenic |
| AATGTGGCTTACATGATCGG+GGG | 0.708447 | 1.3:+73224755 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAATATAATAGTTTAA+GGG | + | chr1.3:73223914-73223933 | None:intergenic | 10.0% |
| !! | AAAGAATATAATAGTTTAAG+GGG | + | chr1.3:73223913-73223932 | None:intergenic | 15.0% |
| !! | CAAAAATAATAAAAATTGGA+TGG | + | chr1.3:73224079-73224098 | None:intergenic | 15.0% |
| !! | CTTGCAAAAATAATAAAAAT+TGG | + | chr1.3:73224083-73224102 | None:intergenic | 15.0% |
| !! | GAAAAGAATATAATAGTTTA+AGG | + | chr1.3:73223915-73223934 | None:intergenic | 15.0% |
| !! | ATTTCAATCAATTCAAACAA+AGG | + | chr1.3:73224587-73224606 | None:intergenic | 20.0% |
| !! | TATTGAAGCAAAAATATTCA+TGG | + | chr1.3:73224023-73224042 | None:intergenic | 20.0% |
| !!! | TGTTATTGATTTTCTTTGAA+TGG | - | chr1.3:73224621-73224640 | MS.gene91286:intron | 20.0% |
| ! | AATATCTACTTAAAATCCTG+AGG | + | chr1.3:73224436-73224455 | None:intergenic | 25.0% |
| ! | CTTCAATATTAATTGTGTCT+TGG | - | chr1.3:73224035-73224054 | MS.gene91286:intron | 25.0% |
| !! | TCTCACATTTAAATGGTTTT+TGG | - | chr1.3:73224492-73224511 | MS.gene91286:intron | 25.0% |
| AATGCAGCACAATTCAAAAT+TGG | + | chr1.3:73224288-73224307 | None:intergenic | 30.0% | |
| ACAACACTTATATTGCTCTA+TGG | - | chr1.3:73223962-73223981 | MS.gene91286:CDS | 30.0% | |
| ACATGTGCACAAACTTTAAT+TGG | - | chr1.3:73224351-73224370 | MS.gene91286:intron | 30.0% | |
| ATAACTACATCTTATGCAAG+AGG | - | chr1.3:73224375-73224394 | MS.gene91286:intron | 30.0% | |
| ATAATAGTTTAAGGGGAATG+TGG | + | chr1.3:73223906-73223925 | None:intergenic | 30.0% | |
| CAAATTCACAAAAAACACAC+AGG | + | chr1.3:73224222-73224241 | None:intergenic | 30.0% | |
| CTACATGTCTCACATTTAAA+TGG | - | chr1.3:73224485-73224504 | MS.gene91286:intron | 30.0% | |
| TTTAATCTTTCATCTCTAGC+AGG | - | chr1.3:73224117-73224136 | MS.gene91286:intron | 30.0% | |
| ! | GATTTTCTTTGAATGGATCA+GGG | - | chr1.3:73224628-73224647 | MS.gene91286:intron | 30.0% |
| ! | TGATTTTCTTTGAATGGATC+AGG | - | chr1.3:73224627-73224646 | MS.gene91286:intron | 30.0% |
| !!! | ACTCAGCAACAAACATTTTT+TGG | + | chr1.3:73224168-73224187 | None:intergenic | 30.0% |
| !!! | GTTTTTTGTGAATTTGCTTC+TGG | - | chr1.3:73224226-73224245 | MS.gene91286:intron | 30.0% |
| ACAAAAAACACACAGGATAG+GGG | + | chr1.3:73224215-73224234 | None:intergenic | 35.0% | |
| CACAAAAAACACACAGGATA+GGG | + | chr1.3:73224216-73224235 | None:intergenic | 35.0% | |
| CATTAAAGGTTGTTTACCTC+AGG | - | chr1.3:73224417-73224436 | MS.gene91286:intron | 35.0% | |
| GATGGAGCAAATTGAAACAA+AGG | + | chr1.3:73224061-73224080 | None:intergenic | 35.0% | |
| TCACAAAAAACACACAGGAT+AGG | + | chr1.3:73224217-73224236 | None:intergenic | 35.0% | |
| TCACTTGGATTGAGCATTAA+AGG | - | chr1.3:73224403-73224422 | MS.gene91286:intron | 35.0% | |
| TGTGCTGCATTATTCATCTA+GGG | - | chr1.3:73224297-73224316 | MS.gene91286:intron | 35.0% | |
| TTGTGCTGCATTATTCATCT+AGG | - | chr1.3:73224296-73224315 | MS.gene91286:intron | 35.0% | |
| !! | GAATTTGCTTCTGGTTTGTA+AGG | - | chr1.3:73224235-73224254 | MS.gene91286:intron | 35.0% |
| !! | TAGCAGGTTTGTTACTTACA+AGG | - | chr1.3:73224258-73224277 | MS.gene91286:intron | 35.0% |
| !!! | CAAGATGTTTTTGGTTGGAT+TGG | - | chr1.3:73224674-73224693 | MS.gene91286:intron | 35.0% |
| AGAAAGTGGGAAAAGGTGAT+TGG | - | chr1.3:73224697-73224716 | MS.gene91286:intron | 40.0% | |
| ATGCAAGAGGAACTTTCACT+TGG | - | chr1.3:73224388-73224407 | MS.gene91286:intron | 40.0% | |
| CAAAAAACACACAGGATAGG+GGG | + | chr1.3:73224214-73224233 | None:intergenic | 40.0% | |
| CATTCTCTCTGAGCAAACAA+TGG | - | chr1.3:73224462-73224481 | MS.gene91286:intron | 40.0% | |
| GTGCTGCATTATTCATCTAG+GGG | - | chr1.3:73224298-73224317 | MS.gene91286:intron | 40.0% | |
| ! | TTGGTTGGATTGGAGAAAGT+GGG | - | chr1.3:73224684-73224703 | MS.gene91286:intron | 40.0% |
| ! | TTTGGTTGGATTGGAGAAAG+TGG | - | chr1.3:73224683-73224702 | MS.gene91286:intron | 40.0% |
| !! | GGAAGAGCACAAGATGTTTT+TGG | - | chr1.3:73224665-73224684 | MS.gene91286:intron | 40.0% |
| !!! | GAGCACAAGATGTTTTTGGT+TGG | - | chr1.3:73224669-73224688 | MS.gene91286:intron | 40.0% |
| AATGTGGCTTACATGATCGG+GGG | + | chr1.3:73223890-73223909 | None:intergenic | 45.0% | |
| GAATGTGGCTTACATGATCG+GGG | + | chr1.3:73223891-73223910 | None:intergenic | 45.0% | |
| GGAATGTGGCTTACATGATC+GGG | + | chr1.3:73223892-73223911 | None:intergenic | 45.0% | |
| GGATTGGAGAAAGTGGGAAA+AGG | - | chr1.3:73224690-73224709 | MS.gene91286:intron | 45.0% | |
| GGGAATGTGGCTTACATGAT+CGG | + | chr1.3:73223893-73223912 | None:intergenic | 45.0% | |
| GTGATTGGAGAGGAATCTCA+AGG | - | chr1.3:73224712-73224731 | MS.gene91286:intron | 45.0% | |
| TTACATGATCGGGGGATTGA+TGG | + | chr1.3:73223882-73223901 | None:intergenic | 45.0% | |
| !! | CTTCTGGTTTGTAAGGTAGC+AGG | - | chr1.3:73224242-73224261 | MS.gene91286:intron | 45.0% |
| GGCTGAGAAGAGACTGCTAT+TGG | - | chr1.3:73224319-73224338 | MS.gene91286:intron | 50.0% | |
| GTGGGAAAAGGTGATTGGAG+AGG | - | chr1.3:73224702-73224721 | MS.gene91286:intron | 50.0% | |
| TGAATGGATCAGGGATTCCG+TGG | - | chr1.3:73224637-73224656 | MS.gene91286:intron | 50.0% | |
| TTGTGCTCTTCCTCAGTCCA+CGG | + | chr1.3:73224657-73224676 | None:intergenic | 50.0% | |
| ! | GCATGACTTGCAACATGCGT+AGG | + | chr1.3:73224759-73224778 | None:intergenic | 50.0% |
| ! | ATCAGGGATTCCGTGGACTG+AGG | - | chr1.3:73224644-73224663 | MS.gene91286:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 73223864 | 73224800 | 73223864 | ID=MS.gene91286 |
| chr1.3 | mRNA | 73223864 | 73224800 | 73223864 | ID=MS.gene91286.t1;Parent=MS.gene91286 |
| chr1.3 | exon | 73224767 | 73224800 | 73224767 | ID=MS.gene91286.t1.exon1;Parent=MS.gene91286.t1 |
| chr1.3 | CDS | 73224767 | 73224800 | 73224767 | ID=cds.MS.gene91286.t1;Parent=MS.gene91286.t1 |
| chr1.3 | exon | 73223864 | 73224015 | 73223864 | ID=MS.gene91286.t1.exon2;Parent=MS.gene91286.t1 |
| chr1.3 | CDS | 73223864 | 73224015 | 73223864 | ID=cds.MS.gene91286.t1;Parent=MS.gene91286.t1 |
| Gene Sequence |
| Protein sequence |
