AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91419


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91419.t1 MTR_1g105010 69.600 125 27 3 1 120 1 119 1.91e-50 156
MS.gene91419.t1 MTR_1g105960 45.783 83 40 1 12 94 2 79 1.04e-20 80.9
MS.gene91419.t1 MTR_8g062700 49.367 79 40 0 1 79 32 110 4.97e-20 85.1
MS.gene91419.t1 MTR_1g104940 47.368 76 40 0 4 79 7 82 5.13e-20 79.7
MS.gene91419.t1 MTR_1g105955 54.930 71 32 0 8 78 13 83 5.16e-20 81.3
MS.gene91419.t1 MTR_1g105955 54.286 70 32 0 10 79 94 163 2.47e-18 76.6
MS.gene91419.t1 MTR_8g062700 49.367 79 40 0 1 79 32 110 5.22e-20 84.7
MS.gene91419.t1 MTR_5g036670 49.315 73 37 0 7 79 39 111 8.52e-19 81.3
MS.gene91419.t1 MTR_1g104937 53.623 69 32 0 11 79 12 80 1.51e-18 75.5
MS.gene91419.t1 MTR_6g008970 44.444 90 43 2 7 96 59 141 3.37e-18 79.7
MS.gene91419.t1 MTR_7g089490 49.275 69 35 0 11 79 16 84 7.05e-18 73.9
MS.gene91419.t1 MTR_1g104960 51.429 70 34 0 10 79 98 167 9.91e-18 75.1
MS.gene91419.t1 MTR_1g104960 48.649 74 38 0 6 79 8 81 7.92e-14 65.1
MS.gene91419.t1 MTR_3g006125 45.882 85 41 2 4 83 173 257 3.32e-16 72.4
MS.gene91419.t1 MTR_3g006065 51.515 66 31 1 10 74 89 154 3.69e-15 68.6
MS.gene91419.t1 MTR_6g008995 41.026 78 46 0 7 84 33 110 5.09e-15 70.5
MS.gene91419.t1 MTR_3g006185 44.186 86 43 2 3 83 90 175 6.93e-15 67.8
MS.gene91419.t1 MTR_8g062350 44.000 75 42 0 4 78 7 81 1.54e-14 67.4
MS.gene91419.t1 MTR_3g006150 51.515 66 31 1 10 74 89 154 1.87e-14 66.2
MS.gene91419.t1 MTR_3g006150 40.845 71 39 1 8 78 5 72 9.73e-11 56.6
MS.gene91419.t1 MTR_7g013410 46.774 62 33 0 18 79 5 66 3.96e-14 64.7
MS.gene91419.t1 MTR_4g133300 47.945 73 38 0 7 79 85 157 1.11e-13 65.9
MS.gene91419.t1 MTR_4g133300 46.970 66 35 0 14 79 175 240 1.83e-13 65.1
MS.gene91419.t1 MTR_4g133300 53.846 52 24 0 27 78 25 76 3.87e-11 58.9
MS.gene91419.t1 MTR_4g133470 46.970 66 35 0 14 79 175 240 1.15e-13 65.5
MS.gene91419.t1 MTR_4g133470 46.575 73 39 0 7 79 85 157 8.99e-13 63.2
MS.gene91419.t1 MTR_4g133470 53.846 52 24 0 27 78 25 76 3.30e-11 58.9
MS.gene91419.t1 MTR_1g091047 49.231 65 32 1 10 73 8 72 1.22e-13 62.0
MS.gene91419.t1 MTR_1g104980 48.485 66 34 0 13 78 1 66 2.48e-13 63.5
MS.gene91419.t1 MTR_1g104980 48.485 66 34 0 14 79 73 138 6.89e-13 62.4
MS.gene91419.t1 MTR_3g006000 52.239 67 29 3 10 74 6 71 1.10e-12 59.7
MS.gene91419.t1 MTR_1g093310 41.667 72 36 1 8 79 6 71 2.52e-12 58.5
MS.gene91419.t1 MTR_1g109550 47.541 61 31 1 18 78 23 82 3.72e-12 59.7
MS.gene91419.t1 MTR_7g013380 44.286 70 37 1 11 78 185 254 7.29e-12 61.2
MS.gene91419.t1 MTR_7g013380 44.615 65 34 1 11 73 99 163 1.30e-11 60.5
MS.gene91419.t1 MTR_7g013380 46.875 64 32 1 12 73 14 77 2.15e-11 59.7
MS.gene91419.t1 MTR_3g006105 42.857 77 43 1 4 79 92 168 7.77e-12 59.7
MS.gene91419.t1 MTR_3g006105 38.554 83 50 1 11 92 8 90 2.50e-11 58.5
MS.gene91419.t1 MTR_2g097830 44.048 84 40 2 10 89 69 149 8.36e-12 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91419.t1 AT1G24190 51.250 80 35 1 4 79 3 82 3.09e-21 88.6
MS.gene91419.t1 AT1G24190 51.250 80 35 1 4 79 3 82 3.25e-21 88.6
MS.gene91419.t1 AT1G24190 51.250 80 35 1 4 79 3 82 3.28e-21 88.6
MS.gene91419.t1 AT1G70060 52.055 73 35 0 7 79 10 82 1.33e-19 84.0
MS.gene91419.t1 AT1G70060 52.055 73 35 0 7 79 10 82 1.33e-19 84.0
MS.gene91419.t1 AT3G01320 47.945 73 38 0 7 79 53 125 2.58e-19 82.8
MS.gene91419.t1 AT3G01320 47.945 73 38 0 7 79 53 125 2.60e-19 82.8
MS.gene91419.t1 AT1G24200 37.302 126 67 4 8 129 11 128 3.14e-18 77.0
MS.gene91419.t1 AT5G15020 45.333 75 41 0 7 81 48 122 4.54e-18 79.3
MS.gene91419.t1 AT5G15020 45.333 75 41 0 7 81 48 122 4.72e-18 79.3
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.34e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.39e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.40e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.40e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.42e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.43e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 235 308 1.46e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.46e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.48e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.48e-15 72.4
MS.gene91419.t1 AT4G12020 46.667 75 39 1 5 79 302 375 1.49e-15 72.4
MS.gene91419.t1 AT1G27250 40.541 74 44 0 7 80 17 90 1.86e-15 68.2
MS.gene91419.t1 AT1G59890 44.928 69 38 0 11 79 45 113 2.67e-15 71.6
MS.gene91419.t1 AT1G59890 44.928 69 38 0 11 79 45 113 2.70e-15 71.6
MS.gene91419.t1 AT1G59890 44.928 69 38 0 11 79 45 113 2.72e-15 71.6
MS.gene91419.t1 AT1G59890 44.928 69 38 0 11 79 45 113 2.75e-15 71.6
MS.gene91419.t1 AT5G15025 38.158 76 47 0 6 81 42 117 1.52e-14 65.5
MS.gene91419.t1 AT1G70030 41.558 77 43 1 7 81 18 94 3.61e-14 64.7
MS.gene91419.t1 AT1G70030 32.812 128 73 4 7 129 7 126 4.31e-14 65.5
MS.gene91419.t1 AT1G70030 41.558 77 43 1 7 81 18 94 7.46e-14 64.3
MS.gene91419.t1 AT1G27220 36.486 74 47 0 7 80 10 83 1.42e-12 61.6
MS.gene91419.t1 AT1G10450 40.580 69 41 0 11 79 33 101 2.19e-12 63.2
MS.gene91419.t1 AT1G10450 40.580 69 41 0 11 79 33 101 2.19e-12 63.2
MS.gene91419.t1 AT1G23810 42.188 64 37 0 8 71 106 169 2.30e-12 62.0
MS.gene91419.t1 AT1G10450 40.580 69 41 0 11 79 84 152 2.33e-12 63.2
MS.gene91419.t1 AT1G27280 38.571 70 43 0 8 77 78 147 3.24e-12 61.6
MS.gene91419.t1 AT1G24230 43.478 69 38 1 6 73 9 77 4.36e-12 61.2
MS.gene91419.t1 AT1G24210 40.000 75 44 1 6 80 9 82 2.08e-11 58.2
MS.gene91419.t1 AT1G24250 42.188 64 37 0 8 71 117 180 2.39e-11 59.3
MS.gene91419.t1 AT5G15040 39.437 71 43 0 11 81 3 73 4.84e-11 55.5
MS.gene91419.t1 AT5G15040 39.437 71 43 0 11 81 3 73 4.84e-11 55.5
MS.gene91419.t1 AT5G15040 39.437 71 43 0 11 81 3 73 4.84e-11 55.5

Find 27 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAAAATATTAGAGATTT+TGG 0.199052 1.3:-71714697 MS.gene91419:CDS
TGAAAGTCCTAAGCGATTAC+AGG 0.315902 1.3:-71714947 MS.gene91419:CDS
AGGATGGAGGAGCTGTTTAA+AGG 0.330709 1.3:-71714891 MS.gene91419:CDS
CTCTTGGCAATGCAGGATAA+AGG 0.369224 1.3:-71715059 MS.gene91419:intron
AAGAACCCACCTGATCGAAT+AGG 0.409769 1.3:-71714771 MS.gene91419:CDS
AAACGCTATTGCTTCTTCCT+TGG 0.431429 1.3:+71715025 None:intergenic
CAGTGATGACACTGGAGAAA+AGG 0.440953 1.3:-71714814 MS.gene91419:CDS
TCACTGATTTGAGATGGCTT+TGG 0.444928 1.3:+71714831 None:intergenic
GGATGGAGGAGCTGTTTAAA+GGG 0.456905 1.3:-71714890 MS.gene91419:CDS
AGAACCCACCTGATCGAATA+GGG 0.457483 1.3:-71714770 MS.gene91419:CDS
ATTTGCGACCCTATTCGATC+AGG 0.474283 1.3:+71714762 None:intergenic
TTGAGATGGCTTTGGCGTAA+AGG 0.479351 1.3:+71714839 None:intergenic
AGTTAAACTTCTGTCTCAAA+AGG 0.482056 1.3:-71714721 MS.gene91419:CDS
TGCGACCCTATTCGATCAGG+TGG 0.509774 1.3:+71714765 None:intergenic
GCTAAAAGAATTGATATAGC+AGG 0.520011 1.3:-71714924 MS.gene91419:CDS
GTGTCATCACTGATTTGAGA+TGG 0.558119 1.3:+71714825 None:intergenic
GAAAGTCCTAAGCGATTACA+GGG 0.564984 1.3:-71714946 MS.gene91419:CDS
AGTTAAGTTTCAAGACAAGA+GGG 0.571152 1.3:-71714988 MS.gene91419:CDS
AAGTTAAGTTTCAAGACAAG+AGG 0.577150 1.3:-71714989 MS.gene91419:CDS
AGCAGGTGTGACAACAAGGA+TGG 0.585519 1.3:-71714907 MS.gene91419:CDS
GCGACCCTATTCGATCAGGT+GGG 0.613931 1.3:+71714766 None:intergenic
ATATAGCAGGTGTGACAACA+AGG 0.623415 1.3:-71714911 MS.gene91419:CDS
TCTCAAATCAGTGATGACAC+TGG 0.643527 1.3:-71714822 MS.gene91419:CDS
AGGTGTGACAACAAGGATGG+AGG 0.648111 1.3:-71714904 MS.gene91419:CDS
TAAAGGTGGTGGTGTAACCA+AGG 0.651615 1.3:-71715042 MS.gene91419:CDS
GCAATGCAGGATAAAGGTGG+TGG 0.669430 1.3:-71715053 MS.gene91419:intron
TTGGCAATGCAGGATAAAGG+TGG 0.685630 1.3:-71715056 MS.gene91419:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAATAAATTAATTTCTCT+TGG - chr1.3:71714918-71714937 MS.gene91419:CDS 15.0%
!!! TGATAAAATATTAGAGATTT+TGG - chr1.3:71715296-71715315 MS.gene91419:intron 15.0%
!!! TAAAATATTAGAGATTTTGG+AGG - chr1.3:71715299-71715318 MS.gene91419:intron 20.0%
!!! TTATTTTTTCCTTCCAATTT+TGG - chr1.3:71714700-71714719 MS.gene91419:CDS 20.0%
!!! TTTTTTTTGAGAGATTGAAT+TGG - chr1.3:71714751-71714770 MS.gene91419:CDS 20.0%
! AGGAGTTTAATTAGTATGAT+AGG + chr1.3:71714875-71714894 None:intergenic 25.0%
! ATTCAAAACAAAGCCAAAAT+TGG + chr1.3:71714716-71714735 None:intergenic 25.0%
! CATATACATGTATAATGTGT+TGG - chr1.3:71714804-71714823 MS.gene91419:CDS 25.0%
!!! ATTTTGGCTTTGTTTTGAAT+TGG - chr1.3:71714716-71714735 MS.gene91419:CDS 25.0%
AAAACAAAGCCAAAATTGGA+AGG + chr1.3:71714712-71714731 None:intergenic 30.0%
AAGTTAAGTTTCAAGACAAG+AGG - chr1.3:71715004-71715023 MS.gene91419:CDS 30.0%
AGTTAAACTTCTGTCTCAAA+AGG - chr1.3:71715272-71715291 MS.gene91419:intron 30.0%
AGTTAAGTTTCAAGACAAGA+GGG - chr1.3:71715005-71715024 MS.gene91419:CDS 30.0%
GCTAAAAGAATTGATATAGC+AGG - chr1.3:71715069-71715088 MS.gene91419:intron 30.0%
GGCATGTCAATCAATTAATA+TGG - chr1.3:71714825-71714844 MS.gene91419:CDS 30.0%
GTAACAAGCATGTTTAATTC+AGG + chr1.3:71714895-71714914 None:intergenic 30.0%
TTAATTTCTCTTGGCAATGC+AGG - chr1.3:71714927-71714946 MS.gene91419:CDS 35.0%
AAACGCTATTGCTTCTTCCT+TGG + chr1.3:71714971-71714990 None:intergenic 40.0%
ATATAGCAGGTGTGACAACA+AGG - chr1.3:71715082-71715101 MS.gene91419:intron 40.0%
GAAAGTCCTAAGCGATTACA+GGG - chr1.3:71715047-71715066 MS.gene91419:CDS 40.0%
TCTCAAATCAGTGATGACAC+TGG - chr1.3:71715171-71715190 MS.gene91419:intron 40.0%
TGAAAGTCCTAAGCGATTAC+AGG - chr1.3:71715046-71715065 MS.gene91419:CDS 40.0%
! TTTTAGCCCTGTAATCGCTT+AGG + chr1.3:71715056-71715075 None:intergenic 40.0%
!! GTGTCATCACTGATTTGAGA+TGG + chr1.3:71715171-71715190 None:intergenic 40.0%
!! TCACTGATTTGAGATGGCTT+TGG + chr1.3:71715165-71715184 None:intergenic 40.0%
AAGAACCCACCTGATCGAAT+AGG - chr1.3:71715222-71715241 MS.gene91419:intron 45.0%
AGAACCCACCTGATCGAATA+GGG - chr1.3:71715223-71715242 MS.gene91419:intron 45.0%
AGGATGGAGGAGCTGTTTAA+AGG - chr1.3:71715102-71715121 MS.gene91419:intron 45.0%
ATTTGCGACCCTATTCGATC+AGG + chr1.3:71715234-71715253 None:intergenic 45.0%
CAGTGATGACACTGGAGAAA+AGG - chr1.3:71715179-71715198 MS.gene91419:intron 45.0%
CTCTTGGCAATGCAGGATAA+AGG - chr1.3:71714934-71714953 MS.gene91419:CDS 45.0%
GGATGGAGGAGCTGTTTAAA+GGG - chr1.3:71715103-71715122 MS.gene91419:intron 45.0%
TTGGCAATGCAGGATAAAGG+TGG - chr1.3:71714937-71714956 MS.gene91419:CDS 45.0%
! TAAAGGTGGTGGTGTAACCA+AGG - chr1.3:71714951-71714970 MS.gene91419:CDS 45.0%
!! TTGAGATGGCTTTGGCGTAA+AGG + chr1.3:71715157-71715176 None:intergenic 45.0%
AGCAGGTGTGACAACAAGGA+TGG - chr1.3:71715086-71715105 MS.gene91419:intron 50.0%
AGGTGTGACAACAAGGATGG+AGG - chr1.3:71715089-71715108 MS.gene91419:intron 50.0%
GCAATGCAGGATAAAGGTGG+TGG - chr1.3:71714940-71714959 MS.gene91419:CDS 50.0%
GCGACCCTATTCGATCAGGT+GGG + chr1.3:71715230-71715249 None:intergenic 55.0%
GGCAACACGTCACACATGCA+TGG - chr1.3:71714846-71714865 MS.gene91419:CDS 55.0%
TGCGACCCTATTCGATCAGG+TGG + chr1.3:71715231-71715250 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 71714677 71715338 71714677 ID=MS.gene91419
chr1.3 mRNA 71714677 71715338 71714677 ID=MS.gene91419.t1;Parent=MS.gene91419
chr1.3 exon 71715336 71715338 71715336 ID=MS.gene91419.t1.exon1;Parent=MS.gene91419.t1
chr1.3 CDS 71715336 71715338 71715336 ID=cds.MS.gene91419.t1;Parent=MS.gene91419.t1
chr1.3 exon 71714677 71715066 71714677 ID=MS.gene91419.t1.exon2;Parent=MS.gene91419.t1
chr1.3 CDS 71714677 71715066 71714677 ID=cds.MS.gene91419.t1;Parent=MS.gene91419.t1
Gene Sequence

>MS.gene91419

ATGGTATGAGCTTTCTTCAATAATTATTTTTTCCTTCCAATTTTGGCTTTGTTTTGAATTGGATTGAATATTTTTTTTTTTTGAGAGATTGAATTGGATTGAGTATAACTAGCATGTAAGTAATTAGCATATACATGTATAATGTGTTGGCATGTCAATCAATTAATATGGCAACACGTCACACATGCATGGTGACCTATCATACTAATTAAACTCCTGAATTAAACATGCTTGTTACTCTCTTAATAAATTAATTTCTCTTGGCAATGCAGGATAAAGGTGGTGGTGTAACCAAGGAAGAAGCAATAGCGTTTGTACTTGATGTAAAAGTTAAGTTTCAAGACAAGAGGGAAAAGTATGATGAATTTTTGAAAGTCCTAAGCGATTACAGGGCTAAAAGAATTGATATAGCAGGTGTGACAACAAGGATGGAGGAGCTGTTTAAAGGGCATTCAGATTTGATTTTGAAATTCAAATCCTTTACGCCAAAGCCATCTCAAATCAGTGATGACACTGGAGAAAAGGAAGAAGACACTGATGAACAGAAGAACCCACCTGATCGAATAGGGTCGCAAATTCAACTTATATCTCAAGAAGTTAAACTTCTGTCTCAAAAGGCTGATAAAATATTAGAGATTTTGGAGGAGAAGAAGAAAGTATAA

Protein sequence

>MS.gene91419.t1

MDKGGGVTKEEAIAFVLDVKVKFQDKREKYDEFLKVLSDYRAKRIDIAGVTTRMEELFKGHSDLILKFKSFTPKPSQISDDTGEKEEDTDEQKNPPDRIGSQIQLISQEVKLLSQKADKILEILEEKKKV