AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91424

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91424.t1 MTR_1g104940 91.803 122 10 0 1 122 1 122 4.72e-78 226
MS.gene91424.t1 MTR_1g104937 67.327 101 31 1 1 101 1 99 4.14e-45 142
MS.gene91424.t1 MTR_1g104960 66.250 80 27 0 14 93 13 92 8.87e-31 108
MS.gene91424.t1 MTR_1g104960 56.410 78 34 0 12 89 97 174 2.21e-25 94.4
MS.gene91424.t1 MTR_1g104980 53.271 107 46 2 17 122 73 176 7.80e-28 100
MS.gene91424.t1 MTR_1g104980 60.294 68 27 0 16 83 1 68 1.65e-23 89.7
MS.gene91424.t1 MTR_5g036670 53.409 88 41 0 4 91 33 120 5.82e-24 95.9
MS.gene91424.t1 MTR_1g104950 69.492 59 16 1 37 93 1 59 2.52e-22 84.0
MS.gene91424.t1 MTR_8g062700 49.474 95 47 1 9 102 37 131 4.82e-22 90.5
MS.gene91424.t1 MTR_8g062700 49.474 95 47 1 9 102 37 131 4.96e-22 90.5
MS.gene91424.t1 MTR_3g006105 45.378 119 59 2 9 121 3 121 5.50e-20 80.9
MS.gene91424.t1 MTR_6g008970 46.512 86 46 0 4 89 53 138 1.30e-19 83.6
MS.gene91424.t1 MTR_1g105960 49.351 77 39 0 15 91 2 78 1.66e-19 77.4
MS.gene91424.t1 MTR_6g008995 45.055 91 49 1 9 99 32 121 1.00e-18 80.9
MS.gene91424.t1 MTR_3g006125 50.515 97 42 2 9 99 84 180 1.50e-18 78.6
MS.gene91424.t1 MTR_3g006125 48.684 76 38 1 14 88 180 255 1.32e-13 65.1
MS.gene91424.t1 MTR_3g006150 48.276 87 42 1 13 99 7 90 4.42e-18 75.5
MS.gene91424.t1 MTR_3g006150 53.521 71 32 1 14 83 90 160 1.01e-14 66.6
MS.gene91424.t1 MTR_3g006185 45.370 108 46 2 9 115 3 98 1.28e-16 72.0
MS.gene91424.t1 MTR_1g105010 50.725 69 34 0 14 82 16 84 1.53e-16 70.1
MS.gene91424.t1 MTR_4g133300 43.956 91 51 0 9 99 84 174 1.64e-16 73.2
MS.gene91424.t1 MTR_4g133300 54.286 70 32 0 17 86 175 244 3.05e-16 72.4
MS.gene91424.t1 MTR_4g133470 54.286 70 32 0 17 86 175 244 2.35e-16 72.4
MS.gene91424.t1 MTR_4g133470 42.857 91 52 0 9 99 84 174 8.18e-15 68.6
MS.gene91424.t1 MTR_1g105955 50.000 72 36 0 15 86 17 88 1.48e-15 69.3
MS.gene91424.t1 MTR_1g105955 49.275 69 35 0 14 82 95 163 7.67e-14 64.7
MS.gene91424.t1 MTR_8g062350 41.053 95 54 1 1 95 1 93 1.89e-15 69.3
MS.gene91424.t1 MTR_3g006065 47.126 87 43 1 13 99 7 90 2.99e-15 68.6
MS.gene91424.t1 MTR_3g006065 50.000 80 37 2 14 90 90 169 2.39e-14 66.2
MS.gene91424.t1 MTR_3g006010 45.570 79 42 1 7 85 3 80 1.09e-14 69.3
MS.gene91424.t1 MTR_7g089490 47.826 69 36 0 14 82 16 84 1.14e-14 65.5
MS.gene91424.t1 MTR_7g013380 40.964 83 45 2 4 84 5 85 5.35e-13 63.9
MS.gene91424.t1 MTR_7g013380 41.429 70 41 0 20 89 193 262 4.92e-12 61.2
MS.gene91424.t1 MTR_7g013410 46.269 67 36 0 18 84 2 68 4.32e-12 59.3
MS.gene91424.t1 MTR_6g008995 48.438 64 32 1 36 99 2 64 1.83e-11 60.1
MS.gene91424.t1 MTR_2g097830 40.909 88 46 2 5 89 58 142 1.91e-11 58.2
MS.gene91424.t1 MTR_1g093310 42.857 70 34 1 16 85 11 74 3.09e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91424.t1 AT3G01320 47.959 98 51 0 5 102 48 145 8.93e-22 89.7
MS.gene91424.t1 AT3G01320 47.959 98 51 0 5 102 48 145 9.11e-22 89.7
MS.gene91424.t1 AT1G70060 54.321 81 37 0 9 89 9 89 2.11e-20 85.9
MS.gene91424.t1 AT1G70060 54.321 81 37 0 9 89 9 89 2.11e-20 85.9
MS.gene91424.t1 AT1G24190 53.086 81 38 0 9 89 9 89 7.38e-20 84.3
MS.gene91424.t1 AT1G24190 53.086 81 38 0 9 89 9 89 8.05e-20 84.0
MS.gene91424.t1 AT1G24190 53.086 81 38 0 9 89 9 89 8.54e-20 84.0
MS.gene91424.t1 AT5G15020 50.667 75 37 0 9 83 47 121 1.25e-17 77.8
MS.gene91424.t1 AT5G15020 50.667 75 37 0 9 83 47 121 1.33e-17 77.8
MS.gene91424.t1 AT5G15025 47.143 70 37 0 14 83 47 116 3.47e-16 69.3
MS.gene91424.t1 AT1G10450 44.186 86 47 1 14 99 84 168 1.27e-15 72.0
MS.gene91424.t1 AT1G10450 44.186 86 47 1 14 99 33 117 1.36e-15 72.0
MS.gene91424.t1 AT1G10450 44.186 86 47 1 14 99 33 117 1.36e-15 72.0
MS.gene91424.t1 AT1G59890 45.455 77 42 0 13 89 44 120 1.67e-15 71.6
MS.gene91424.t1 AT1G59890 45.455 77 42 0 13 89 44 120 1.69e-15 71.6
MS.gene91424.t1 AT1G59890 45.455 77 42 0 13 89 44 120 1.75e-15 71.6
MS.gene91424.t1 AT1G59890 45.455 77 42 0 13 89 44 120 1.82e-15 71.6
MS.gene91424.t1 AT1G70030 45.455 77 42 0 7 83 15 91 1.29e-14 65.5
MS.gene91424.t1 AT1G70030 41.489 94 54 1 7 100 15 107 4.15e-14 64.7
MS.gene91424.t1 AT1G70030 44.444 81 45 0 7 87 4 84 4.30e-14 65.1
MS.gene91424.t1 AT5G15040 46.053 76 39 1 13 88 2 75 1.06e-13 62.0
MS.gene91424.t1 AT5G15040 46.053 76 39 1 13 88 2 75 1.06e-13 62.0
MS.gene91424.t1 AT5G15040 46.053 76 39 1 13 88 2 75 1.06e-13 62.0
MS.gene91424.t1 AT1G24200 46.377 69 37 0 14 82 14 82 1.63e-13 64.3
MS.gene91424.t1 AT5G35610 40.000 95 54 1 8 102 4 95 2.11e-13 63.2
MS.gene91424.t1 AT1G27220 42.029 69 40 0 14 82 14 82 5.72e-12 60.1
MS.gene91424.t1 AT1G23810 36.782 87 54 1 14 100 109 194 8.23e-11 57.4

Find 17 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTCTGTATCAATTCTT+TGG 0.241773 1.3:-71678065 None:intergenic
TGCTTCAGCTTCACCAATCT+TGG 0.305827 1.3:-71677953 None:intergenic
AGAACTTCTGAAAGATTTCA+AGG 0.359735 1.3:+71678041 MS.gene91424:CDS
ACCAATCTTGGCATTTACTC+TGG 0.415002 1.3:-71677941 None:intergenic
AGCTGTGAAAGAAGGAGTTA+TGG 0.477622 1.3:+71678083 MS.gene91424:CDS
TCCAGAGTAAATGCCAAGAT+TGG 0.492118 1.3:+71677940 MS.gene91424:CDS
ACATGAAATCTCACTTCCAT+TGG 0.500530 1.3:+71678164 MS.gene91424:CDS
CAGAATGATGATGAACTTCA+AGG 0.516640 1.3:+71678231 MS.gene91424:CDS
GATACAGAAGCTGTGAAAGA+AGG 0.535981 1.3:+71678075 MS.gene91424:CDS
TTCAACATCTTCATGCCACC+AGG 0.561140 1.3:+71678141 MS.gene91424:CDS
GATGATCAACAACAAGGTGA+TGG 0.565922 1.3:+71678189 MS.gene91424:CDS
TGTTGTTGATCATCATCCAA+TGG 0.574340 1.3:-71678180 None:intergenic
GATGATGAACTTCAAGGTGA+TGG 0.578269 1.3:+71678237 MS.gene91424:CDS
TGAACTTCAAGGTGATGGAT+TGG 0.587737 1.3:+71678242 MS.gene91424:CDS
GGAAGTGAGATTTCATGTCC+TGG 0.614261 1.3:-71678159 None:intergenic
TTGGATGATGATCAACAACA+AGG 0.710928 1.3:+71678183 MS.gene91424:CDS
AGTGAGATTTCATGTCCTGG+TGG 0.738423 1.3:-71678156 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAACTTCTGAAAGATTTCA+AGG + chr1.3:71678041-71678060 MS.gene91424:CDS 30.0%
ACATGAAATCTCACTTCCAT+TGG + chr1.3:71678164-71678183 MS.gene91424:CDS 35.0%
CAGCTTCTGTATCAATTCTT+TGG - chr1.3:71678068-71678087 None:intergenic 35.0%
TGTTGTTGATCATCATCCAA+TGG - chr1.3:71678183-71678202 None:intergenic 35.0%
TTGGATGATGATCAACAACA+AGG + chr1.3:71678183-71678202 MS.gene91424:CDS 35.0%
! CAGAATGATGATGAACTTCA+AGG + chr1.3:71678231-71678250 MS.gene91424:CDS 35.0%
ACCAATCTTGGCATTTACTC+TGG - chr1.3:71677944-71677963 None:intergenic 40.0%
AGCTGTGAAAGAAGGAGTTA+TGG + chr1.3:71678083-71678102 MS.gene91424:CDS 40.0%
GATACAGAAGCTGTGAAAGA+AGG + chr1.3:71678075-71678094 MS.gene91424:CDS 40.0%
GATGATCAACAACAAGGTGA+TGG + chr1.3:71678189-71678208 MS.gene91424:CDS 40.0%
GATGATGAACTTCAAGGTGA+TGG + chr1.3:71678237-71678256 MS.gene91424:CDS 40.0%
TCCAGAGTAAATGCCAAGAT+TGG + chr1.3:71677940-71677959 MS.gene91424:CDS 40.0%
!! TGAACTTCAAGGTGATGGAT+TGG + chr1.3:71678242-71678261 MS.gene91424:CDS 40.0%
AGTGAGATTTCATGTCCTGG+TGG - chr1.3:71678159-71678178 None:intergenic 45.0%
GGAAGTGAGATTTCATGTCC+TGG - chr1.3:71678162-71678181 None:intergenic 45.0%
TGCTTCAGCTTCACCAATCT+TGG - chr1.3:71677956-71677975 None:intergenic 45.0%
TTCAACATCTTCATGCCACC+AGG + chr1.3:71678141-71678160 MS.gene91424:CDS 45.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 71677931 71678299 71677931 ID=MS.gene91424
chr1.3 mRNA 71677931 71678299 71677931 ID=MS.gene91424.t1;Parent=MS.gene91424
chr1.3 exon 71677931 71678299 71677931 ID=MS.gene91424.t1.exon1;Parent=MS.gene91424.t1
chr1.3 CDS 71677931 71678299 71677931 ID=cds.MS.gene91424.t1;Parent=MS.gene91424.t1
Gene Sequence

>MS.gene91424

ATGAGAAGATCCAGAGTAAATGCCAAGATTGGTGAAGCTGAAGCACTTGCGTTTATAATGAAAGTGAAATATGCGTTTCAAGATAAGAGAGAAAAGTATGAAGAATTTTTAGAACTTCTGAAAGATTTCAAGGTCCAAAGAATTGATACAGAAGCTGTGAAAGAAGGAGTTATGGAGTTGTTTAAAGAACATCAATATTTGATTTCAAGATTCAACATCTTCATGCCACCAGGACATGAAATCTCACTTCCATTGGATGATGATCAACAACAAGGTGATGGATTAGAGTTCAAAGATGAACAGAATGATGATGAACTTCAAGGTGATGGATTGGCACTCAAAGATGAGCAGAAAAATGATGAAGAATAA
Protein sequence

>MS.gene91424.t1

MRRSRVNAKIGEAEALAFIMKVKYAFQDKREKYEEFLELLKDFKVQRIDTEAVKEGVMELFKEHQYLISRFNIFMPPGHEISLPLDDDQQQGDGLEFKDEQNDDELQGDGLALKDEQKNDEE