AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91425

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91425.t1 MTR_1g104937 96.429 112 4 0 1 112 1 112 1.75e-74 216
MS.gene91425.t1 MTR_1g104940 62.376 101 36 1 1 99 1 101 9.46e-40 129
MS.gene91425.t1 MTR_1g104960 55.357 112 47 1 4 112 5 116 3.78e-33 114
MS.gene91425.t1 MTR_1g104960 59.459 74 30 0 13 86 100 173 1.75e-24 91.7
MS.gene91425.t1 MTR_1g104980 61.765 68 26 0 14 81 1 68 2.41e-24 91.3
MS.gene91425.t1 MTR_1g104980 55.128 78 35 0 14 91 72 149 6.44e-22 85.1
MS.gene91425.t1 MTR_1g105960 47.436 78 41 0 13 90 2 79 1.12e-18 75.1
MS.gene91425.t1 MTR_8g062700 48.718 78 40 0 10 87 40 117 2.64e-18 79.3
MS.gene91425.t1 MTR_8g062700 48.718 78 40 0 10 87 40 117 2.75e-18 79.3
MS.gene91425.t1 MTR_1g104950 59.322 59 22 1 35 91 1 59 1.29e-17 71.6
MS.gene91425.t1 MTR_5g036670 42.500 80 46 0 10 89 41 120 1.38e-17 77.0
MS.gene91425.t1 MTR_6g008970 47.436 78 41 0 10 87 61 138 1.94e-17 76.6
MS.gene91425.t1 MTR_3g006105 50.602 83 40 1 10 91 6 88 3.03e-17 73.2
MS.gene91425.t1 MTR_6g008995 43.023 86 48 1 12 97 37 121 3.83e-16 72.8
MS.gene91425.t1 MTR_3g006125 53.333 75 34 1 10 83 87 161 7.11e-16 70.9
MS.gene91425.t1 MTR_3g006125 43.421 76 42 1 12 86 180 255 6.48e-11 57.4
MS.gene91425.t1 MTR_3g006185 51.613 93 40 2 10 97 6 98 7.16e-16 69.7
MS.gene91425.t1 MTR_1g105955 50.000 72 36 0 13 84 17 88 7.43e-16 69.7
MS.gene91425.t1 MTR_1g105955 47.826 69 36 0 12 80 95 163 8.18e-12 58.9
MS.gene91425.t1 MTR_1g105010 48.718 78 37 1 12 89 16 90 2.52e-15 66.6
MS.gene91425.t1 MTR_4g133300 39.394 99 60 0 12 110 89 187 1.55e-14 67.4
MS.gene91425.t1 MTR_4g133300 44.286 70 39 0 15 84 175 244 1.31e-11 59.3
MS.gene91425.t1 MTR_7g013380 43.038 79 43 1 5 81 6 84 1.76e-14 67.8
MS.gene91425.t1 MTR_7g013380 42.308 78 43 1 12 87 185 262 3.01e-11 58.9
MS.gene91425.t1 MTR_4g133470 40.000 100 58 2 12 110 89 187 2.14e-13 64.3
MS.gene91425.t1 MTR_4g133470 44.286 70 39 0 15 84 175 244 1.19e-11 59.7
MS.gene91425.t1 MTR_3g006150 41.379 87 48 1 11 97 7 90 1.67e-12 60.5
MS.gene91425.t1 MTR_3g006150 50.000 66 32 1 10 74 88 153 2.05e-12 60.1
MS.gene91425.t1 MTR_8g062350 42.254 71 41 0 12 82 14 84 2.74e-12 60.5
MS.gene91425.t1 MTR_3g006065 45.000 80 41 2 10 86 88 167 8.66e-12 58.9
MS.gene91425.t1 MTR_7g089490 40.580 69 41 0 12 80 16 84 2.31e-11 56.6
MS.gene91425.t1 MTR_3g006010 44.000 75 41 1 10 83 6 80 2.95e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91425.t1 AT3G01320 46.316 95 49 1 4 96 47 141 1.50e-19 82.8
MS.gene91425.t1 AT3G01320 46.316 95 49 1 4 96 47 141 1.51e-19 82.8
MS.gene91425.t1 AT5G15020 48.684 76 37 1 12 87 52 125 8.45e-16 72.0
MS.gene91425.t1 AT5G15020 48.684 76 37 1 12 87 52 125 8.45e-16 72.0
MS.gene91425.t1 AT1G70060 44.872 78 43 0 10 87 12 89 1.40e-15 71.6
MS.gene91425.t1 AT1G70060 44.872 78 43 0 10 87 12 89 1.40e-15 71.6
MS.gene91425.t1 AT1G24190 44.737 76 42 0 10 85 12 87 4.36e-15 70.1
MS.gene91425.t1 AT1G24190 44.737 76 42 0 10 85 12 87 4.40e-15 70.1
MS.gene91425.t1 AT1G24190 44.737 76 42 0 10 85 12 87 4.54e-15 70.1
MS.gene91425.t1 AT5G15025 45.455 77 40 1 12 88 47 121 1.27e-14 65.1
MS.gene91425.t1 AT5G15040 46.753 77 39 1 10 86 1 75 1.57e-14 63.9
MS.gene91425.t1 AT5G15040 46.753 77 39 1 10 86 1 75 1.57e-14 63.9
MS.gene91425.t1 AT5G15040 46.753 77 39 1 10 86 1 75 1.57e-14 63.9
MS.gene91425.t1 AT1G24200 47.143 70 37 0 11 80 13 82 2.18e-14 66.2
MS.gene91425.t1 AT1G27220 42.466 73 42 0 8 80 10 82 2.97e-14 65.5
MS.gene91425.t1 AT1G23810 38.636 88 53 1 5 92 102 188 2.36e-13 63.9
MS.gene91425.t1 AT1G59890 37.179 78 49 0 12 89 45 122 1.69e-12 62.8
MS.gene91425.t1 AT1G59890 38.158 76 47 0 12 87 45 120 1.72e-12 62.8
MS.gene91425.t1 AT1G59890 37.179 78 49 0 12 89 45 122 1.72e-12 62.8
MS.gene91425.t1 AT1G59890 38.158 76 47 0 12 87 45 120 1.79e-12 62.8
MS.gene91425.t1 AT1G24250 39.773 88 52 1 5 92 113 199 1.88e-12 61.6
MS.gene91425.t1 AT1G24230 51.515 66 31 1 10 74 12 77 2.14e-12 61.6
MS.gene91425.t1 AT1G24230 41.935 62 36 0 15 76 89 150 6.07e-12 60.5
MS.gene91425.t1 AT1G24210 38.961 77 45 1 1 75 1 77 3.51e-12 59.7
MS.gene91425.t1 AT5G35610 42.500 80 43 1 20 99 18 94 5.04e-12 59.3
MS.gene91425.t1 AT1G70030 40.230 87 51 1 12 98 22 107 7.78e-12 58.2
MS.gene91425.t1 AT1G70030 40.230 87 51 1 12 98 22 107 1.41e-11 57.8
MS.gene91425.t1 AT1G70030 40.230 87 51 1 12 98 11 96 2.15e-11 57.8
MS.gene91425.t1 AT1G27260 37.209 86 53 1 7 92 88 172 2.27e-11 58.5
MS.gene91425.t1 AT1G27250 39.706 68 41 0 12 79 21 88 3.08e-11 56.6
MS.gene91425.t1 AT1G10450 36.047 86 54 1 12 97 84 168 5.64e-11 58.5
MS.gene91425.t1 AT1G27280 43.478 69 39 0 7 75 76 144 7.07e-11 57.4
MS.gene91425.t1 AT1G10450 36.047 86 54 1 12 97 33 117 7.11e-11 58.2
MS.gene91425.t1 AT1G10450 36.047 86 54 1 12 97 33 117 7.11e-11 58.2

Find 18 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACACTGCTTCACCAATCT+TGG 0.362408 1.3:-71674408 None:intergenic
GAAAATTCTAGATGATTTCT+CGG 0.377556 1.3:+71674490 MS.gene91425:CDS
TGAAGCAGTGTCGTTTGTTA+TGG 0.413384 1.3:+71674418 MS.gene91425:CDS
TAAAACATGTGTTTCAAGAT+AGG 0.427626 1.3:+71674444 MS.gene91425:CDS
AGCTGCTCGATCAATTCTTT+CGG 0.448838 1.3:-71674513 None:intergenic
CGCAGAGTAGATGCCAAGAT+TGG 0.484518 1.3:+71674395 MS.gene91425:CDS
TATTGTTGCTGATCATCAAA+TGG 0.491825 1.3:-71674701 None:intergenic
TTCAACATCTTCTTGCCACC+AGG 0.516818 1.3:+71674590 MS.gene91425:CDS
AACATGTGTTTCAAGATAGG+AGG 0.520327 1.3:+71674447 MS.gene91425:CDS
GAGTTGTTAAAAGATCATAA+AGG 0.526893 1.3:+71674554 MS.gene91425:CDS
ACAAGAAATCTCACTTACAT+TGG 0.536387 1.3:+71674613 MS.gene91425:CDS
GATCGAGCAGCTGTGAAAGA+AGG 0.542586 1.3:+71674524 MS.gene91425:CDS
GTAAGTGAGATTTCTTGTCC+TGG 0.568387 1.3:-71674608 None:intergenic
ACATGTGTTTCAAGATAGGA+GGG 0.571664 1.3:+71674448 MS.gene91425:CDS
GATGATGAACAACAAGGTGA+TGG 0.587348 1.3:+71674638 MS.gene91425:CDS
AGCTGTGAAAGAAGGAATGA+CGG 0.588334 1.3:+71674532 MS.gene91425:CDS
AGTGAGATTTCTTGTCCTGG+TGG 0.701974 1.3:-71674605 None:intergenic
TTGGATGATGATGAACAACA+AGG 0.780029 1.3:+71674632 MS.gene91425:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAAAATTCTAGATGATTTCT+CGG + chr1.3:71674490-71674509 MS.gene91425:CDS 25.0%
! GAGTTGTTAAAAGATCATAA+AGG + chr1.3:71674554-71674573 MS.gene91425:CDS 25.0%
! TAAAACATGTGTTTCAAGAT+AGG + chr1.3:71674444-71674463 MS.gene91425:CDS 25.0%
ACAAGAAATCTCACTTACAT+TGG + chr1.3:71674613-71674632 MS.gene91425:CDS 30.0%
TATTGTTGCTGATCATCAAA+TGG - chr1.3:71674704-71674723 None:intergenic 30.0%
AACATGTGTTTCAAGATAGG+AGG + chr1.3:71674447-71674466 MS.gene91425:CDS 35.0%
ACATGTGTTTCAAGATAGGA+GGG + chr1.3:71674448-71674467 MS.gene91425:CDS 35.0%
! TTGGATGATGATGAACAACA+AGG + chr1.3:71674632-71674651 MS.gene91425:CDS 35.0%
AGCTGCTCGATCAATTCTTT+CGG - chr1.3:71674516-71674535 None:intergenic 40.0%
AGCTGTGAAAGAAGGAATGA+CGG + chr1.3:71674532-71674551 MS.gene91425:CDS 40.0%
GATGATGAACAACAAGGTGA+TGG + chr1.3:71674638-71674657 MS.gene91425:CDS 40.0%
GTAAGTGAGATTTCTTGTCC+TGG - chr1.3:71674611-71674630 None:intergenic 40.0%
!! TGAAGCAGTGTCGTTTGTTA+TGG + chr1.3:71674418-71674437 MS.gene91425:CDS 40.0%
AGTGAGATTTCTTGTCCTGG+TGG - chr1.3:71674608-71674627 None:intergenic 45.0%
TTCAACATCTTCTTGCCACC+AGG + chr1.3:71674590-71674609 MS.gene91425:CDS 45.0%
CGACACTGCTTCACCAATCT+TGG - chr1.3:71674411-71674430 None:intergenic 50.0%
CGCAGAGTAGATGCCAAGAT+TGG + chr1.3:71674395-71674414 MS.gene91425:CDS 50.0%
GATCGAGCAGCTGTGAAAGA+AGG + chr1.3:71674524-71674543 MS.gene91425:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 71674386 71674724 71674386 ID=MS.gene91425
chr1.3 mRNA 71674386 71674724 71674386 ID=MS.gene91425.t1;Parent=MS.gene91425
chr1.3 exon 71674386 71674724 71674386 ID=MS.gene91425.t1.exon1;Parent=MS.gene91425.t1
chr1.3 CDS 71674386 71674724 71674386 ID=cds.MS.gene91425.t1;Parent=MS.gene91425.t1
Gene Sequence

>MS.gene91425

ATGAGGAGACGCAGAGTAGATGCCAAGATTGGTGAAGCAGTGTCGTTTGTTATGGAAGTAAAACATGTGTTTCAAGATAGGAGGGAGAAGTATGATGAGTTTTTGAAAATTCTAGATGATTTCTCGGCCGAAAGAATTGATCGAGCAGCTGTGAAAGAAGGAATGACGGAGTTGTTAAAAGATCATAAAGGTTTAATTTCAAGATTCAACATCTTCTTGCCACCAGGACAAGAAATCTCACTTACATTGGATGATGATGAACAACAAGGTGATGGATTAGCATTCGAAGATGAACAGAAACATGAAATCTCACTTCCATTTGATGATCAGCAACAATAA
Protein sequence

>MS.gene91425.t1

MRRRRVDAKIGEAVSFVMEVKHVFQDRREKYDEFLKILDDFSAERIDRAAVKEGMTELLKDHKGLISRFNIFLPPGQEISLTLDDDEQQGDGLAFEDEQKHEISLPFDDQQQ