AlfalfaGEDB | MS.gene91997

M. sativa cultivar XinJiangDaYe / MS.gene91997

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91997.t1	XP_007136352.1	97.9	48	1	0	31	78	225	272	3.20E-17	97.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91997.t1	Q9SGN7	81.2	48	9	0	31	78	230	277	5.6e-15	81.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91997.t1	G7J573	97.9	48	1	0	31	78	239	286	2.3e-17	97.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91997.t1	MTR_3g100680	97.917	48	1	0	31	78	239	286	2.25e-26	100
MS.gene91997.t1	MTR_4g044663	79.167	48	10	0	31	78	216	263	4.53e-20	82.8
MS.gene91997.t1	MTR_2g098910	56.250	48	21	0	31	78	695	742	3.02e-12	60.5
MS.gene91997.t1	MTR_7g061660	63.265	49	12	2	33	77	254	300	3.61e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91997.t1	AT1G28390	81.250	48	9	31	78	230	277	7.80e-21	84.7
MS.gene91997.t1	AT1G28390	81.250	48	9	31	78	230	277	1.05e-19	81.6
MS.gene91997.t1	AT3G51990	70.833	48	14	31	78	229	276	4.68e-17	73.9
MS.gene91997.t1	AT5G23170	64.583	48	17	31	78	195	242	2.54e-15	68.9
MS.gene91997.t1	AT3G59420	58.333	48	20	31	78	685	732	4.62e-13	62.8
MS.gene91997.t1	AT3G45920	52.000	50	24	31	80	50	99	3.73e-11	56.2

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGTGATGTGTTCAGTTT+TGG	0.264787	7.3:+79374962	MS.gene91997:CDS
GTCCTCCCTCGGTGGTTGAT+TGG	0.298122	7.3:+79375038	MS.gene91997:CDS
TCCTCCCTCGGTGGTTGATT+GGG	0.329826	7.3:+79375039	MS.gene91997:CDS
ATTCTTAGCGGTTCACATGA+CGG	0.528051	7.3:-79374845	None:intergenic
ATCTCGCCGGCGAAGATGAG+AGG	0.530988	7.3:+79374897	MS.gene91997:CDS
TCCCTCGGTGGTTGATTGGG+CGG	0.535840	7.3:+79375042	MS.gene91997:CDS
TCCTCCGTCGCAAGATAGCA+TGG	0.553902	7.3:-79374929	None:intergenic
CAACACAGCAAGATTCTTAG+CGG	0.561306	7.3:-79374857	None:intergenic
GCCCAATCAACCACCGAGGG+AGG	0.573647	7.3:-79375040	None:intergenic
TGTTAATTATAGTCCTCCCT+CGG	0.576616	7.3:+79375027	MS.gene91997:CDS
AAGATTCCTCTCATCTTCGC+CGG	0.578273	7.3:-79374903	None:intergenic
ACCGCCCAATCAACCACCGA+GGG	0.599914	7.3:-79375043	None:intergenic
TTGTTGTTGGAGATTATCAG+TGG	0.603371	7.3:+79374989	MS.gene91997:CDS
TGTTGTTGGAGATTATCAGT+GGG	0.611840	7.3:+79374990	MS.gene91997:CDS
AAATCACCTCTTCTAATCAA+CGG	0.621197	7.3:-79375068	None:intergenic
TGATCCATGCTATCTTGCGA+CGG	0.622949	7.3:+79374925	MS.gene91997:CDS
TCCATGCTATCTTGCGACGG+AGG	0.633531	7.3:+79374928	MS.gene91997:CDS
TAATTATAGTCCTCCCTCGG+TGG	0.662707	7.3:+79375030	MS.gene91997:CDS
GAGAGTTCTGCATATCTCGC+CGG	0.664248	7.3:+79374884	MS.gene91997:CDS
AACCGCCCAATCAACCACCG+AGG	0.701635	7.3:-79375044	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TGTTAATTATAGTCCTCCCT+CGG	+	chr7.3:79375027-79375046	MS.gene91997:CDS	35.0%
	TGTTGTTGGAGATTATCAGT+GGG	+	chr7.3:79374990-79375009	MS.gene91997:CDS	35.0%
!	AAGAGTGATGTGTTCAGTTT+TGG	+	chr7.3:79374962-79374981	MS.gene91997:CDS	35.0%
!	AGAGTGATGTGTTCAGTTTT+GGG	+	chr7.3:79374963-79374982	MS.gene91997:CDS	35.0%
!	TTGTTGTTGGAGATTATCAG+TGG	+	chr7.3:79374989-79375008	MS.gene91997:CDS	35.0%
	ATTCTTAGCGGTTCACATGA+CGG	-	chr7.3:79374848-79374867	None:intergenic	40.0%
	CAACACAGCAAGATTCTTAG+CGG	-	chr7.3:79374860-79374879	None:intergenic	40.0%
!!!	CAGTTTTGGGATCTTGTTGT+TGG	+	chr7.3:79374976-79374995	MS.gene91997:CDS	40.0%
	AAGATTCCTCTCATCTTCGC+CGG	-	chr7.3:79374906-79374925	None:intergenic	45.0%
	TAATTATAGTCCTCCCTCGG+TGG	+	chr7.3:79375030-79375049	MS.gene91997:CDS	45.0%
	TGATCCATGCTATCTTGCGA+CGG	+	chr7.3:79374925-79374944	MS.gene91997:CDS	45.0%
	GAGAGTTCTGCATATCTCGC+CGG	+	chr7.3:79374884-79374903	MS.gene91997:CDS	50.0%
	TCCATGCTATCTTGCGACGG+AGG	+	chr7.3:79374928-79374947	MS.gene91997:CDS	55.0%
	TCCTCCCTCGGTGGTTGATT+GGG	+	chr7.3:79375039-79375058	MS.gene91997:CDS	55.0%
	TCCTCCGTCGCAAGATAGCA+TGG	-	chr7.3:79374932-79374951	None:intergenic	55.0%
	AACCGCCCAATCAACCACCG+AGG	-	chr7.3:79375047-79375066	None:intergenic	60.0%
	ACCGCCCAATCAACCACCGA+GGG	-	chr7.3:79375046-79375065	None:intergenic	60.0%
	ATCTCGCCGGCGAAGATGAG+AGG	+	chr7.3:79374897-79374916	MS.gene91997:CDS	60.0%
	GTCCTCCCTCGGTGGTTGAT+TGG	+	chr7.3:79375038-79375057	MS.gene91997:CDS	60.0%
	TCCCTCGGTGGTTGATTGGG+CGG	+	chr7.3:79375042-79375061	MS.gene91997:CDS	60.0%
	GCCCAATCAACCACCGAGGG+AGG	-	chr7.3:79375043-79375062	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	79374833	79375078	79374833	ID=MS.gene91997
chr7.3	mRNA	79374833	79375078	79374833	ID=MS.gene91997.t1;Parent=MS.gene91997
chr7.3	exon	79374833	79375078	79374833	ID=MS.gene91997.t1.exon1;Parent=MS.gene91997.t1
chr7.3	CDS	79374833	79375078	79374833	ID=cds.MS.gene91997.t1;Parent=MS.gene91997.t1

Gene Sequence	>MS.gene91997 ATGAATCTCTCGCCGTCATGTGAACCGCTAAGAATCTTGCTGTGTTGTTCTGAGAGTTCTGCATATCTCGCCGGCGAAGATGAGAGGAATCTTGATCCATGCTATCTTGCGACGGAGGATCTTAGCGCGAAGAGTGATGTGTTCAGTTTTGGGATCTTGTTGTTGGAGATTATCAGTGGGAGAAATGCGATTGATGTTAATTATAGTCCTCCCTCGGTGGTTGATTGGGCGGTTGCCGTTGATTAG
Protein sequence	>MS.gene91997.t1 MNLSPSCEPLRILLCCSESSAYLAGEDERNLDPCYLATEDLSAKSDVFSFGILLLEIISGRNAIDVNYSPPSVVDWAVAVD