AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92032

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92032.t1 MTR_0376s0030 76.898 303 41 3 1 277 1 300 6.31e-153 446
MS.gene92032.t1 MTR_0376s0030 85.500 200 24 2 256 450 379 578 1.77e-113 345
MS.gene92032.t1 MTR_6g044690 65.333 300 75 2 5 277 5 302 5.66e-115 349
MS.gene92032.t1 MTR_6g044690 69.231 221 57 4 222 436 351 566 5.74e-96 300
MS.gene92032.t1 MTR_2g054460 72.523 222 57 1 54 275 1 218 6.44e-105 320
MS.gene92032.t1 MTR_2g054460 65.333 225 71 3 217 437 246 467 1.08e-94 293
MS.gene92032.t1 MTR_6g043960 66.071 224 69 3 217 436 264 484 4.79e-95 295
MS.gene92032.t1 MTR_6g043960 67.727 220 65 1 64 277 1 220 6.85e-93 289
MS.gene92032.t1 MTR_1g035420 63.492 189 56 5 268 450 478 659 6.88e-70 234
MS.gene92032.t1 MTR_1g035420 44.099 322 118 8 5 289 107 403 6.25e-67 226
MS.gene92032.t1 MTR_8g070070 59.043 188 63 5 268 449 589 768 9.69e-68 230
MS.gene92032.t1 MTR_8g070070 46.907 194 67 3 113 270 299 492 2.82e-44 165
MS.gene92032.t1 MTR_8g070075 55.801 181 70 4 259 433 573 749 5.97e-61 212
MS.gene92032.t1 MTR_8g070075 47.208 197 68 3 110 270 289 485 3.51e-46 171
MS.gene92032.t1 MTR_8g071030 51.269 197 82 5 259 449 560 748 8.08e-60 208
MS.gene92032.t1 MTR_8g071030 37.143 280 134 5 37 280 209 482 9.56e-48 175
MS.gene92032.t1 MTR_1g035400 57.471 174 64 4 280 450 412 578 6.27e-55 192
MS.gene92032.t1 MTR_1g035400 42.248 258 103 4 57 279 63 309 2.31e-53 188
MS.gene92032.t1 MTR_6g044000 68.333 120 38 0 64 183 1 120 1.47e-48 164
MS.gene92032.t1 MTR_6g044000 91.228 57 5 0 381 437 116 172 1.70e-30 116
MS.gene92032.t1 MTR_7g445600 29.375 320 154 8 115 432 106 355 1.86e-33 129
MS.gene92032.t1 MTR_4g010830 34.444 180 108 3 259 432 774 949 4.63e-28 118
MS.gene92032.t1 MTR_4g010830 35.754 179 80 6 111 258 507 681 3.12e-19 91.3
MS.gene92032.t1 MTR_5g013420 35.000 180 107 4 259 432 883 1058 2.04e-27 116
MS.gene92032.t1 MTR_5g013420 37.778 180 77 6 111 259 615 790 6.05e-22 99.8
MS.gene92032.t1 MTR_5g013420 35.000 180 107 4 259 432 883 1058 2.24e-27 116
MS.gene92032.t1 MTR_5g013420 37.778 180 77 6 111 259 615 790 5.95e-22 99.8
MS.gene92032.t1 MTR_3g011440 35.542 166 104 2 273 435 470 635 6.75e-27 114
MS.gene92032.t1 MTR_3g011440 33.186 226 111 9 71 256 125 350 1.44e-21 98.2
MS.gene92032.t1 MTR_7g088370 37.629 194 90 6 94 257 219 411 1.08e-24 107
MS.gene92032.t1 MTR_7g088370 33.696 184 108 5 270 449 522 695 6.43e-21 96.3
MS.gene92032.t1 MTR_6g061270 31.183 186 114 4 259 433 482 664 1.27e-24 107
MS.gene92032.t1 MTR_6g061270 32.609 184 87 6 111 258 196 378 3.04e-16 81.6
MS.gene92032.t1 MTR_7g084090 32.432 185 113 4 259 433 545 727 2.74e-24 106
MS.gene92032.t1 MTR_7g084090 32.642 193 92 7 103 258 250 441 3.44e-17 84.7
MS.gene92032.t1 MTR_8g042750 35.669 157 97 3 280 433 502 657 1.41e-23 104
MS.gene92032.t1 MTR_8g042750 39.456 147 78 5 99 235 187 332 2.14e-18 88.2
MS.gene92032.t1 MTR_4g010550 36.872 179 78 6 111 258 21 195 1.30e-20 94.4
MS.gene92032.t1 MTR_4g010550 31.285 179 99 4 259 432 274 433 8.30e-20 92.0
MS.gene92032.t1 MTR_7g445710 30.409 171 99 4 279 432 345 512 7.95e-19 89.4
MS.gene92032.t1 MTR_7g450730 31.818 154 93 3 279 432 333 474 1.68e-18 88.2
MS.gene92032.t1 MTR_7g450730 42.857 105 50 4 129 232 121 216 3.83e-11 65.5
MS.gene92032.t1 MTR_7g445530 30.409 171 99 4 279 432 345 512 1.71e-18 88.2
MS.gene92032.t1 MTR_7g052480 30.481 187 116 6 258 433 343 526 3.53e-18 87.4
MS.gene92032.t1 MTR_7g084090 32.642 193 92 7 103 258 250 441 1.57e-17 85.5
MS.gene92032.t1 MTR_6g061200 33.152 184 86 7 111 258 196 378 1.46e-16 82.8
MS.gene92032.t1 MTR_5g018850 27.586 174 105 4 259 429 1325 1480 1.85e-13 73.6
MS.gene92032.t1 MTR_4g131630 59.615 52 18 1 379 430 1 49 2.38e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92032.t1 AT4G13460 53.535 198 79 4 259 450 460 650 2.15e-61 211
MS.gene92032.t1 AT4G13460 40.392 255 102 7 58 278 141 379 1.58e-43 162
MS.gene92032.t1 AT4G13460 53.535 198 79 4 259 450 460 650 2.15e-61 211
MS.gene92032.t1 AT4G13460 40.392 255 102 7 58 278 141 379 1.58e-43 162
MS.gene92032.t1 AT2G33290 52.020 198 81 5 259 450 462 651 1.64e-58 203
MS.gene92032.t1 AT2G33290 45.078 193 75 5 109 270 181 373 1.79e-39 150
MS.gene92032.t1 AT2G33290 52.020 198 81 5 259 450 462 651 1.64e-58 203
MS.gene92032.t1 AT2G33290 45.078 193 75 5 109 270 181 373 1.79e-39 150
MS.gene92032.t1 AT2G33290 52.020 198 81 5 259 450 462 651 1.64e-58 203
MS.gene92032.t1 AT2G33290 45.078 193 75 5 109 270 181 373 1.79e-39 150
MS.gene92032.t1 AT2G33290 52.020 198 81 5 259 450 462 651 1.64e-58 203
MS.gene92032.t1 AT2G33290 45.078 193 75 5 109 270 181 373 1.79e-39 150
MS.gene92032.t1 AT2G22740 31.963 219 127 6 221 432 556 759 5.44e-25 108
MS.gene92032.t1 AT2G22740 37.037 189 75 7 110 258 305 489 2.08e-20 94.7
MS.gene92032.t1 AT2G22740 31.963 219 127 6 221 432 556 759 5.44e-25 108
MS.gene92032.t1 AT2G22740 37.037 189 75 7 110 258 305 489 2.08e-20 94.7
MS.gene92032.t1 AT2G35160 32.768 177 89 4 259 432 614 763 6.39e-24 105
MS.gene92032.t1 AT2G35160 38.462 169 64 7 127 258 357 522 2.35e-19 91.7
MS.gene92032.t1 AT2G35160 32.768 177 89 4 259 432 614 763 6.39e-24 105
MS.gene92032.t1 AT2G35160 38.462 169 64 7 127 258 357 522 2.35e-19 91.7
MS.gene92032.t1 AT2G35160 32.768 177 89 4 259 432 614 763 6.39e-24 105
MS.gene92032.t1 AT2G35160 38.462 169 64 7 127 258 357 522 2.35e-19 91.7
MS.gene92032.t1 AT2G35160 32.768 177 89 4 259 432 614 763 6.39e-24 105
MS.gene92032.t1 AT2G35160 38.462 169 64 7 127 258 357 522 2.35e-19 91.7
MS.gene92032.t1 AT2G35160 32.768 177 89 4 259 432 578 727 7.54e-24 105
MS.gene92032.t1 AT2G35160 38.462 169 64 7 127 258 321 486 2.22e-19 91.7
MS.gene92032.t1 AT5G04940 37.168 226 98 8 62 256 150 362 4.23e-22 99.8
MS.gene92032.t1 AT5G04940 33.742 163 100 3 275 433 481 639 3.92e-19 90.5
MS.gene92032.t1 AT5G04940 37.168 226 98 8 62 256 150 362 4.23e-22 99.8
MS.gene92032.t1 AT5G04940 33.742 163 100 3 275 433 481 639 3.92e-19 90.5
MS.gene92032.t1 AT5G47150 34.450 209 92 7 88 255 120 324 9.50e-21 93.2
MS.gene92032.t1 AT5G13960 32.065 184 114 4 259 433 279 460 3.19e-20 93.6
MS.gene92032.t1 AT5G13960 32.544 169 77 5 127 258 7 175 9.05e-17 82.8
MS.gene92032.t1 AT5G13960 32.065 184 114 4 259 433 413 594 3.25e-20 94.0
MS.gene92032.t1 AT5G13960 34.135 208 97 8 90 258 103 309 1.74e-19 91.7
MS.gene92032.t1 AT1G73100 34.946 186 110 5 256 433 456 638 5.36e-20 93.2
MS.gene92032.t1 AT1G73100 30.516 213 113 7 77 256 150 360 4.70e-18 87.4
MS.gene92032.t1 AT2G24740 29.365 252 166 7 8 250 180 428 3.00e-18 88.2
MS.gene92032.t1 AT2G24740 32.768 177 105 5 281 449 573 743 7.48e-18 86.7
MS.gene92032.t1 AT1G17770 30.726 179 112 4 261 433 488 660 1.39e-16 82.8
MS.gene92032.t1 AT1G17770 31.788 151 93 4 92 235 186 333 3.97e-15 78.2
MS.gene92032.t1 AT5G47160 41.593 113 52 4 131 233 258 366 7.51e-14 73.6
MS.gene92032.t1 AT5G47160 41.593 113 52 4 131 233 258 366 7.51e-14 73.6
MS.gene92032.t1 AT5G47160 41.593 113 52 4 131 233 258 366 7.51e-14 73.6
MS.gene92032.t1 AT2G23740 26.087 184 105 4 259 434 1197 1357 2.52e-13 73.2
MS.gene92032.t1 AT2G23740 26.087 184 105 4 259 434 1197 1357 2.52e-13 73.2
MS.gene92032.t1 AT2G23740 26.087 184 105 4 259 434 1197 1357 2.52e-13 73.2
MS.gene92032.t1 AT2G23740 26.087 184 105 4 259 434 1190 1350 2.80e-13 72.8

Find 98 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACATATCGCGAGCTTTC+CGG 0.209031 3.2:-37224328 None:intergenic
TTCTCTTTGATCACCATAAT+TGG 0.217083 3.2:+37224205 MS.gene92032:CDS
GGTGACTTTATTACCTATTC+TGG 0.241715 3.2:+37223193 MS.gene92032:CDS
TTACAATTATTCTCTTCTTC+TGG 0.304252 3.2:-37222851 None:intergenic
TAGAACGATGGCGCGTGATA+TGG 0.304655 3.2:-37222636 None:intergenic
TTGGAGGTTTCCCCATCTTA+TGG 0.306382 3.2:+37224224 MS.gene92032:CDS
GATGGGGAAACCTCCAATTA+TGG 0.336282 3.2:-37224218 None:intergenic
GATATGGAAGGATTTGGATT+TGG 0.339474 3.2:-37222620 None:intergenic
GCGCGTGATATGGAAGGATT+TGG 0.339831 3.2:-37222626 None:intergenic
CCAGTTCCTGGTGTTTGTAT+TGG 0.341157 3.2:+37223016 MS.gene92032:CDS
TGGAAGAAGTGAATAATAGT+AGG 0.355057 3.2:+37222915 MS.gene92032:CDS
ATGAATCTAGTTGGGGAGTT+AGG 0.362684 3.2:+37223890 MS.gene92032:CDS
AGGATCATACTACCCATCTT+AGG 0.371623 3.2:-37223452 None:intergenic
AAAATCATTGGTCCAGTTCC+TGG 0.372423 3.2:+37223004 MS.gene92032:CDS
TAGATCTAATGAATCTAGTT+GGG 0.379002 3.2:+37223882 MS.gene92032:CDS
TGCAATATGAGATTGATGTT+AGG 0.379723 3.2:+37223278 MS.gene92032:CDS
TTCTAATTTGATTCGCGTGT+CGG 0.380074 3.2:+37222823 MS.gene92032:CDS
TCTAATTTGATTCGCGTGTC+GGG 0.385608 3.2:+37222824 MS.gene92032:CDS
CATCATAATATGAGTACTAA+TGG 0.386201 3.2:+37223115 MS.gene92032:CDS
GTCAATGTCGTGAGATGGTC+TGG 0.393246 3.2:+37222786 MS.gene92032:CDS
AGAAACCGTGTGACACAAAA+AGG 0.393465 3.2:+37223835 MS.gene92032:intron
CAATAGTCGCACTCAAGAAT+GGG 0.401700 3.2:+37224044 MS.gene92032:CDS
TATCCAATGCAAAATCTAAA+GGG 0.408021 3.2:-37224113 None:intergenic
CTTATCACCTTGGCCAGAAT+AGG 0.413018 3.2:-37223206 None:intergenic
GCAATATGAGATTGATGTTA+GGG 0.414210 3.2:+37223279 MS.gene92032:CDS
ACTAATTGAACAAACACATT+AGG 0.416299 3.2:-37224183 None:intergenic
TAGTTGGGGAGTTAGGTCTT+TGG 0.416783 3.2:+37223897 MS.gene92032:CDS
CTTGTTAGCACTGCGATGGA+TGG 0.418511 3.2:+37222755 MS.gene92032:CDS
GATTCTTCTTCTTATCACCT+TGG 0.428001 3.2:-37223216 None:intergenic
TCAATAGTCGCACTCAAGAA+TGG 0.430857 3.2:+37224043 MS.gene92032:CDS
TCATGGAAAAGATGCTGGTT+TGG 0.431818 3.2:+37224293 MS.gene92032:CDS
GAAGATCGTATGCTTAAGAT+AGG 0.441162 3.2:+37223479 MS.gene92032:CDS
GGTTCAAGGGCAACCTAAGA+TGG 0.444689 3.2:+37223439 MS.gene92032:CDS
TATCATGATAAGAAAATCAT+TGG 0.455213 3.2:+37222992 MS.gene92032:CDS
TTAGATCTAATGAATCTAGT+TGG 0.458265 3.2:+37223881 MS.gene92032:CDS
CCATCTTATGGTATTTGCAA+TGG 0.461992 3.2:+37224236 MS.gene92032:CDS
GAACAAACACATTAGGAGTT+GGG 0.465286 3.2:-37224176 None:intergenic
ATATCCAATGCAAAATCTAA+AGG 0.465523 3.2:-37224114 None:intergenic
CCAATACAAACACCAGGAAC+TGG 0.467377 3.2:-37223016 None:intergenic
TTGTTAGCACTGCGATGGAT+GGG 0.472906 3.2:+37222756 MS.gene92032:CDS
TGTTGTTGCTACTACTGGTA+AGG 0.474142 3.2:+37223327 MS.gene92032:CDS
AGAACTGAAATGTGTGTGAT+TGG 0.483156 3.2:+37223055 MS.gene92032:CDS
CCATTGCAAATACCATAAGA+TGG 0.485061 3.2:-37224236 None:intergenic
AGCTCGCGATATGTAATTGA+AGG 0.486438 3.2:+37224334 MS.gene92032:CDS
GGAAGAAGTGAATAATAGTA+GGG 0.486889 3.2:+37222916 MS.gene92032:CDS
TGAACAAACACATTAGGAGT+TGG 0.487323 3.2:-37224177 None:intergenic
CACAATCACACTAGTAGCAA+TGG 0.490809 3.2:-37223143 None:intergenic
CATTGCAAATACCATAAGAT+GGG 0.495661 3.2:-37224235 None:intergenic
TCTTTGATCACCATAATTGG+AGG 0.499967 3.2:+37224208 MS.gene92032:CDS
AGACCATCTCACGACATTGA+CGG 0.500062 3.2:-37222784 None:intergenic
TAAATGCGTCAGCTAGGATA+AGG 0.500251 3.2:-37223921 None:intergenic
CTTGATCATGGAAAAGATGC+TGG 0.501654 3.2:+37224288 MS.gene92032:CDS
TGAGTTATGTTCACCATCCA+TGG 0.507207 3.2:-37223995 None:intergenic
TTCATCTTTGATGAACAAGT+TGG 0.507571 3.2:+37222895 MS.gene92032:CDS
TCAAGACTAAGCTCTCTCAT+TGG 0.507939 3.2:-37224270 None:intergenic
GATGGTGTTGTTGCTACTAC+TGG 0.510952 3.2:+37223322 MS.gene92032:CDS
AGAAGAAGAGAATAATTGTA+AGG 0.511249 3.2:+37222853 MS.gene92032:CDS
TACTACAACAACACTCTGGC+GGG 0.514983 3.2:+37222680 MS.gene92032:CDS
CTGCCTAGTTAAAACAAGTC+CGG 0.526238 3.2:-37223956 None:intergenic
TACATGGTCAACCACAAGCT+GGG 0.533174 3.2:+37223080 MS.gene92032:CDS
ATTCGATGTGTTGAGTATGA+TGG 0.535451 3.2:+37223304 MS.gene92032:CDS
AGACTAAGCTCTCTCATTGG+AGG 0.536032 3.2:-37224267 None:intergenic
AGAGAGCTTAGTCTTGATCA+TGG 0.539359 3.2:+37224276 MS.gene92032:CDS
ACAATCACACTAGTAGCAAT+GGG 0.545383 3.2:-37223142 None:intergenic
TGTTAGCACTGCGATGGATG+GGG 0.553713 3.2:+37222757 MS.gene92032:CDS
CTCGTTGGGAACTAGAACGA+TGG 0.553850 3.2:-37222648 None:intergenic
TGGGCTATGACTGATATAGT+TGG 0.557321 3.2:-37224157 None:intergenic
ATGTGTGTGATTGGTTTACA+TGG 0.563933 3.2:+37223064 MS.gene92032:CDS
TTACATGGTCAACCACAAGC+TGG 0.564688 3.2:+37223079 MS.gene92032:CDS
TTTATTACCTATTCTGGCCA+AGG 0.564751 3.2:+37223199 MS.gene92032:CDS
AAGAAGAATCAAAAGTTGCA+AGG 0.572931 3.2:+37223229 MS.gene92032:CDS
GCAAGGAGGCAATCTTGCTA+TGG 0.586494 3.2:+37223246 MS.gene92032:CDS
ACTAGTGTGATTGTGTCTAG+TGG 0.586855 3.2:+37223151 MS.gene92032:CDS
GTTCAAGGGCAACCTAAGAT+GGG 0.587188 3.2:+37223440 MS.gene92032:CDS
CACATATAAATGCGTCAGCT+AGG 0.592036 3.2:-37223927 None:intergenic
GGGGCTTGTTAGCACTGCGA+TGG 0.594139 3.2:+37222751 MS.gene92032:CDS
GCATTTATATGTGAGTTCAC+CGG 0.594362 3.2:+37223937 MS.gene92032:CDS
GGTTTGGATGAGAAATTGAC+CGG 0.596703 3.2:+37224309 MS.gene92032:CDS
AGATCTAATGAATCTAGTTG+GGG 0.604580 3.2:+37223883 MS.gene92032:CDS
AAATTACTACAACAACACTC+TGG 0.605973 3.2:+37222676 MS.gene92032:CDS
TCACCGTCAATGTCGTGAGA+TGG 0.606005 3.2:+37222781 MS.gene92032:CDS
GGATATGATGATGTTGATCA+AGG 0.607077 3.2:+37223172 MS.gene92032:CDS
TCACACTAGTAGCAATGGGT+TGG 0.609530 3.2:-37223138 None:intergenic
ATCCAATGCAAAATCTAAAG+GGG 0.614062 3.2:-37224112 None:intergenic
TTACTACAACAACACTCTGG+CGG 0.623780 3.2:+37222679 MS.gene92032:CDS
TAAGCATACGATCTTCCTCA+AGG 0.625836 3.2:-37223472 None:intergenic
ACGATGGCGCGTGATATGGA+AGG 0.627777 3.2:-37222632 None:intergenic
GATGGGTAGTATGATCCTTG+AGG 0.638084 3.2:+37223457 MS.gene92032:CDS
AAGCTTAAAGATCATAACAA+GGG 0.638888 3.2:+37222731 MS.gene92032:CDS
TCCAATGCAAAATCTAAAGG+GGG 0.642759 3.2:-37224111 None:intergenic
AGCTTAAAGATCATAACAAG+GGG 0.645722 3.2:+37222732 MS.gene92032:CDS
ATTGCAAATACCATAAGATG+GGG 0.649831 3.2:-37224234 None:intergenic
AAGAATGGGGTGATTTATCG+AGG 0.651095 3.2:+37224058 MS.gene92032:CDS
ACATCACCAATACAAACACC+AGG 0.663968 3.2:-37223022 None:intergenic
GAAGCTTAAAGATCATAACA+AGG 0.672214 3.2:+37222730 MS.gene92032:CDS
AATAGTCGCACTCAAGAATG+GGG 0.683786 3.2:+37224045 MS.gene92032:CDS
GAATAATCAATCCCAGCTTG+TGG 0.713794 3.2:-37223091 None:intergenic
AAGAATCAAAAGTTGCAAGG+AGG 0.730139 3.2:+37223232 MS.gene92032:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTATAAGATAATTAAATGT+TGG + chr3.2:37223366-37223385 MS.gene92032:intron 15.0%
!! TATCATGATAAGAAAATCAT+TGG + chr3.2:37222992-37223011 MS.gene92032:CDS 20.0%
!! TTTCTCATAATATTTCCAAT+GGG + chr3.2:37223531-37223550 MS.gene92032:intron 20.0%
!!! ATTTTTCCTTATAGTAAAAG+TGG + chr3.2:37223745-37223764 MS.gene92032:intron 20.0%
!!! TTATAAGTTTTTGTTATCGA+GGG + chr3.2:37223418-37223437 MS.gene92032:intron 20.0%
!!! TTTATAAGTTTTTGTTATCG+AGG + chr3.2:37223417-37223436 MS.gene92032:intron 20.0%
!!! TTTTTCCTTATAGTAAAAGT+GGG + chr3.2:37223746-37223765 MS.gene92032:intron 20.0%
! AAGCTTAAAGATCATAACAA+GGG + chr3.2:37222731-37222750 MS.gene92032:CDS 25.0%
! ACTAATTGAACAAACACATT+AGG - chr3.2:37224186-37224205 None:intergenic 25.0%
! AGAAGAAGAGAATAATTGTA+AGG + chr3.2:37222853-37222872 MS.gene92032:CDS 25.0%
! ATATCCAATGCAAAATCTAA+AGG - chr3.2:37224117-37224136 None:intergenic 25.0%
! ATTAAATGTTGGTGTGAAAA+TGG + chr3.2:37223377-37223396 MS.gene92032:intron 25.0%
! CATCATAATATGAGTACTAA+TGG + chr3.2:37223115-37223134 MS.gene92032:CDS 25.0%
! CTTTCTCATAATATTTCCAA+TGG + chr3.2:37223530-37223549 MS.gene92032:intron 25.0%
! TAGATCTAATGAATCTAGTT+GGG + chr3.2:37223882-37223901 MS.gene92032:CDS 25.0%
! TATCCAATGCAAAATCTAAA+GGG - chr3.2:37224116-37224135 None:intergenic 25.0%
! TTACAATTATTCTCTTCTTC+TGG - chr3.2:37222854-37222873 None:intergenic 25.0%
! TTAGATCTAATGAATCTAGT+TGG + chr3.2:37223881-37223900 MS.gene92032:CDS 25.0%
! TTATGAGAAAGAACATACAT+AGG - chr3.2:37223521-37223540 None:intergenic 25.0%
!!! TGCATTTTTTGAAACAAGAA+TGG + chr3.2:37223718-37223737 MS.gene92032:intron 25.0%
AAATTACTACAACAACACTC+TGG + chr3.2:37222676-37222695 MS.gene92032:CDS 30.0%
AAGAAGAATCAAAAGTTGCA+AGG + chr3.2:37223229-37223248 MS.gene92032:CDS 30.0%
AGATCTAATGAATCTAGTTG+GGG + chr3.2:37223883-37223902 MS.gene92032:CDS 30.0%
AGCTTAAAGATCATAACAAG+GGG + chr3.2:37222732-37222751 MS.gene92032:CDS 30.0%
ATCCAATGCAAAATCTAAAG+GGG - chr3.2:37224115-37224134 None:intergenic 30.0%
ATTGCAAATACCATAAGATG+GGG - chr3.2:37224237-37224256 None:intergenic 30.0%
CATTGCAAATACCATAAGAT+GGG - chr3.2:37224238-37224257 None:intergenic 30.0%
GAAGCTTAAAGATCATAACA+AGG + chr3.2:37222730-37222749 MS.gene92032:CDS 30.0%
GCAATATGAGATTGATGTTA+GGG + chr3.2:37223279-37223298 MS.gene92032:CDS 30.0%
GGAAGAAGTGAATAATAGTA+GGG + chr3.2:37222916-37222935 MS.gene92032:CDS 30.0%
TCATAATATTTCCAATGGGA+AGG + chr3.2:37223535-37223554 MS.gene92032:intron 30.0%
TGCAAAGAACAACTACAAAA+AGG - chr3.2:37223811-37223830 None:intergenic 30.0%
TGCAATATGAGATTGATGTT+AGG + chr3.2:37223278-37223297 MS.gene92032:CDS 30.0%
TGGAAGAAGTGAATAATAGT+AGG + chr3.2:37222915-37222934 MS.gene92032:CDS 30.0%
TTCATCTTTGATGAACAAGT+TGG + chr3.2:37222895-37222914 MS.gene92032:CDS 30.0%
TTCTCTTTGATCACCATAAT+TGG + chr3.2:37224205-37224224 MS.gene92032:CDS 30.0%
!! ACTGGTAAGGTTTTTGTTTA+TGG + chr3.2:37223340-37223359 MS.gene92032:intron 30.0%
!! AGAATCCCACTTTTACTATA+AGG - chr3.2:37223754-37223773 None:intergenic 30.0%
!! CTGGTAAGGTTTTTGTTTAT+GGG + chr3.2:37223341-37223360 MS.gene92032:intron 30.0%
!! GTGTTGTTGTAGTAATTTTG+TGG - chr3.2:37222674-37222693 None:intergenic 30.0%
AAAAGTGGGATTCTTCTGAA+AGG + chr3.2:37223760-37223779 MS.gene92032:intron 35.0%
AAAATCTAAAGGGGGATATG+AGG - chr3.2:37224106-37224125 None:intergenic 35.0%
AAATCTAAAGGGGGATATGA+GGG - chr3.2:37224105-37224124 None:intergenic 35.0%
AAGAATCAAAAGTTGCAAGG+AGG + chr3.2:37223232-37223251 MS.gene92032:CDS 35.0%
ACAATCACACTAGTAGCAAT+GGG - chr3.2:37223145-37223164 None:intergenic 35.0%
AGAACTGAAATGTGTGTGAT+TGG + chr3.2:37223055-37223074 MS.gene92032:CDS 35.0%
ATTCGATGTGTTGAGTATGA+TGG + chr3.2:37223304-37223323 MS.gene92032:CDS 35.0%
CACAAAAAGGTCTTGAACAT+AGG + chr3.2:37223848-37223867 MS.gene92032:CDS 35.0%
CCATCTTATGGTATTTGCAA+TGG + chr3.2:37224236-37224255 MS.gene92032:CDS 35.0%
CCATTGCAAATACCATAAGA+TGG - chr3.2:37224239-37224258 None:intergenic 35.0%
CGACTATTGAAAAATCGACT+TGG - chr3.2:37224033-37224052 None:intergenic 35.0%
GAACAAACACATTAGGAGTT+GGG - chr3.2:37224179-37224198 None:intergenic 35.0%
GAAGATCGTATGCTTAAGAT+AGG + chr3.2:37223479-37223498 MS.gene92032:CDS 35.0%
GATATGGAAGGATTTGGATT+TGG - chr3.2:37222623-37222642 None:intergenic 35.0%
GATTCTTCTTCTTATCACCT+TGG - chr3.2:37223219-37223238 None:intergenic 35.0%
GCATTTATATGTGAGTTCAC+CGG + chr3.2:37223937-37223956 MS.gene92032:CDS 35.0%
GGTGACTTTATTACCTATTC+TGG + chr3.2:37223193-37223212 MS.gene92032:CDS 35.0%
TCCAATGCAAAATCTAAAGG+GGG - chr3.2:37224114-37224133 None:intergenic 35.0%
TCTTTGATCACCATAATTGG+AGG + chr3.2:37224208-37224227 MS.gene92032:CDS 35.0%
TGAACAAACACATTAGGAGT+TGG - chr3.2:37224180-37224199 None:intergenic 35.0%
TTTATTACCTATTCTGGCCA+AGG + chr3.2:37223199-37223218 MS.gene92032:CDS 35.0%
! AAAGTGGGATTCTTCTGAAA+GGG + chr3.2:37223761-37223780 MS.gene92032:intron 35.0%
! GGATATGATGATGTTGATCA+AGG + chr3.2:37223172-37223191 MS.gene92032:CDS 35.0%
! TAGTAATTTTGTGGCTCGTT+GGG - chr3.2:37222665-37222684 None:intergenic 35.0%
! TTCTAATTTGATTCGCGTGT+CGG + chr3.2:37222823-37222842 MS.gene92032:CDS 35.0%
!! ATGTGTGTGATTGGTTTACA+TGG + chr3.2:37223064-37223083 MS.gene92032:CDS 35.0%
!! GCAAGTGGAAATTTTAACCA+TGG + chr3.2:37223978-37223997 MS.gene92032:CDS 35.0%
!! GTGGAAATTTTAACCATGGA+TGG + chr3.2:37223982-37224001 MS.gene92032:CDS 35.0%
!! TTTTGTTATCGAGGGTTCAA+GGG + chr3.2:37223426-37223445 MS.gene92032:CDS 35.0%
!! TTTTTGTTATCGAGGGTTCA+AGG + chr3.2:37223425-37223444 MS.gene92032:CDS 35.0%
AAAATCATTGGTCCAGTTCC+TGG + chr3.2:37223004-37223023 MS.gene92032:CDS 40.0%
AAGAATGGGGTGATTTATCG+AGG + chr3.2:37224058-37224077 MS.gene92032:CDS 40.0%
AATAGTCGCACTCAAGAATG+GGG + chr3.2:37224045-37224064 MS.gene92032:CDS 40.0%
ACATCACCAATACAAACACC+AGG - chr3.2:37223025-37223044 None:intergenic 40.0%
ACTAGTGTGATTGTGTCTAG+TGG + chr3.2:37223151-37223170 MS.gene92032:CDS 40.0%
AGAAACCGTGTGACACAAAA+AGG + chr3.2:37223835-37223854 MS.gene92032:intron 40.0%
AGGATCATACTACCCATCTT+AGG - chr3.2:37223455-37223474 None:intergenic 40.0%
ATGAATCTAGTTGGGGAGTT+AGG + chr3.2:37223890-37223909 MS.gene92032:CDS 40.0%
ATTACATATCGCGAGCTTTC+CGG - chr3.2:37224331-37224350 None:intergenic 40.0%
CAATAGTCGCACTCAAGAAT+GGG + chr3.2:37224044-37224063 MS.gene92032:CDS 40.0%
CACAATCACACTAGTAGCAA+TGG - chr3.2:37223146-37223165 None:intergenic 40.0%
CACATATAAATGCGTCAGCT+AGG - chr3.2:37223930-37223949 None:intergenic 40.0%
CTGCCTAGTTAAAACAAGTC+CGG - chr3.2:37223959-37223978 None:intergenic 40.0%
CTTGATCATGGAAAAGATGC+TGG + chr3.2:37224288-37224307 MS.gene92032:CDS 40.0%
GAATAATCAATCCCAGCTTG+TGG - chr3.2:37223094-37223113 None:intergenic 40.0%
GGTTTGGATGAGAAATTGAC+CGG + chr3.2:37224309-37224328 MS.gene92032:CDS 40.0%
TAAATGCGTCAGCTAGGATA+AGG - chr3.2:37223924-37223943 None:intergenic 40.0%
TAAGCATACGATCTTCCTCA+AGG - chr3.2:37223475-37223494 None:intergenic 40.0%
TATGCAATTCGAGTATCTCC+CGG + chr3.2:37223607-37223626 MS.gene92032:intron 40.0%
TCAAGACTAAGCTCTCTCAT+TGG - chr3.2:37224273-37224292 None:intergenic 40.0%
TCAATAGTCGCACTCAAGAA+TGG + chr3.2:37224043-37224062 MS.gene92032:CDS 40.0%
TGAGTTATGTTCACCATCCA+TGG - chr3.2:37223998-37224017 None:intergenic 40.0%
TGGGCTATGACTGATATAGT+TGG - chr3.2:37224160-37224179 None:intergenic 40.0%
TGGTGTGAAAATGGTGAATC+TGG + chr3.2:37223386-37223405 MS.gene92032:intron 40.0%
TGTTGTTGCTACTACTGGTA+AGG + chr3.2:37223327-37223346 MS.gene92032:CDS 40.0%
TTACTACAACAACACTCTGG+CGG + chr3.2:37222679-37222698 MS.gene92032:CDS 40.0%
! CAAGACCTTTTTGTGTCACA+CGG - chr3.2:37223843-37223862 None:intergenic 40.0%
! GTAGTAATTTTGTGGCTCGT+TGG - chr3.2:37222666-37222685 None:intergenic 40.0%
! TCATGGAAAAGATGCTGGTT+TGG + chr3.2:37224293-37224312 MS.gene92032:CDS 40.0%
! TCCCCCTTTAGATTTTGCAT+TGG + chr3.2:37224110-37224129 MS.gene92032:CDS 40.0%
! TCTAATTTGATTCGCGTGTC+GGG + chr3.2:37222824-37222843 MS.gene92032:CDS 40.0%
!! AGAGAGCTTAGTCTTGATCA+TGG + chr3.2:37224276-37224295 MS.gene92032:CDS 40.0%
!!! TCACCGGACTTGTTTTAACT+AGG + chr3.2:37223953-37223972 MS.gene92032:CDS 40.0%
!!! TGTTTTAACTAGGCAGCAAG+TGG + chr3.2:37223963-37223982 MS.gene92032:CDS 40.0%
ACCAATGTTCTCCTTCCCAT+TGG - chr3.2:37223549-37223568 None:intergenic 45.0%
AGACCATCTCACGACATTGA+CGG - chr3.2:37222787-37222806 None:intergenic 45.0%
AGACTAAGCTCTCTCATTGG+AGG - chr3.2:37224270-37224289 None:intergenic 45.0%
CCAATACAAACACCAGGAAC+TGG - chr3.2:37223019-37223038 None:intergenic 45.0%
CCAGTTCCTGGTGTTTGTAT+TGG + chr3.2:37223016-37223035 MS.gene92032:CDS 45.0%
CTTATCACCTTGGCCAGAAT+AGG - chr3.2:37223209-37223228 None:intergenic 45.0%
GATGGGGAAACCTCCAATTA+TGG - chr3.2:37224221-37224240 None:intergenic 45.0%
GATGGGTAGTATGATCCTTG+AGG + chr3.2:37223457-37223476 MS.gene92032:CDS 45.0%
GGGAGATACTCGAATTGCAT+AGG - chr3.2:37223608-37223627 None:intergenic 45.0%
GTTCAAGGGCAACCTAAGAT+GGG + chr3.2:37223440-37223459 MS.gene92032:CDS 45.0%
TACATGGTCAACCACAAGCT+GGG + chr3.2:37223080-37223099 MS.gene92032:CDS 45.0%
TACTACAACAACACTCTGGC+GGG + chr3.2:37222680-37222699 MS.gene92032:CDS 45.0%
TAGTTGGGGAGTTAGGTCTT+TGG + chr3.2:37223897-37223916 MS.gene92032:CDS 45.0%
TCACACTAGTAGCAATGGGT+TGG - chr3.2:37223141-37223160 None:intergenic 45.0%
TCCAATGGGAAGGAGAACAT+TGG + chr3.2:37223545-37223564 MS.gene92032:intron 45.0%
TTACATGGTCAACCACAAGC+TGG + chr3.2:37223079-37223098 MS.gene92032:CDS 45.0%
TTGGAGGTTTCCCCATCTTA+TGG + chr3.2:37224224-37224243 MS.gene92032:CDS 45.0%
TTGTTAGCACTGCGATGGAT+GGG + chr3.2:37222756-37222775 MS.gene92032:CDS 45.0%
! GAGTGTTGTGAATGCGTTGA+TGG + chr3.2:37223694-37223713 MS.gene92032:intron 45.0%
!! GATGGTGTTGTTGCTACTAC+TGG + chr3.2:37223322-37223341 MS.gene92032:CDS 45.0%
CTCGTTGGGAACTAGAACGA+TGG - chr3.2:37222651-37222670 None:intergenic 50.0%
CTTGTTAGCACTGCGATGGA+TGG + chr3.2:37222755-37222774 MS.gene92032:CDS 50.0%
GCAAGGAGGCAATCTTGCTA+TGG + chr3.2:37223246-37223265 MS.gene92032:CDS 50.0%
GCGCGTGATATGGAAGGATT+TGG - chr3.2:37222629-37222648 None:intergenic 50.0%
GGTTCAAGGGCAACCTAAGA+TGG + chr3.2:37223439-37223458 MS.gene92032:CDS 50.0%
GTCAATGTCGTGAGATGGTC+TGG + chr3.2:37222786-37222805 MS.gene92032:CDS 50.0%
TAGAACGATGGCGCGTGATA+TGG - chr3.2:37222639-37222658 None:intergenic 50.0%
TCACCGTCAATGTCGTGAGA+TGG + chr3.2:37222781-37222800 MS.gene92032:CDS 50.0%
TGTTAGCACTGCGATGGATG+GGG + chr3.2:37222757-37222776 MS.gene92032:CDS 50.0%
TTCCGCAATTCGTGTTGCCT+CGG + chr3.2:37223638-37223657 MS.gene92032:intron 50.0%
! GTGGGATTCTTCTGAAAGGG+AGG + chr3.2:37223764-37223783 MS.gene92032:intron 50.0%
AATTGCGGAAAGCAGCCTCC+GGG - chr3.2:37223628-37223647 None:intergenic 55.0%
ACGATGGCGCGTGATATGGA+AGG - chr3.2:37222635-37222654 None:intergenic 55.0%
CTCCGAGGCAACACGAATTG+CGG - chr3.2:37223643-37223662 None:intergenic 55.0%
GAATTGCGGAAAGCAGCCTC+CGG - chr3.2:37223629-37223648 None:intergenic 55.0%
GCAATTCGAGTATCTCCCGG+AGG + chr3.2:37223610-37223629 MS.gene92032:intron 55.0%
!! GGGGCTTGTTAGCACTGCGA+TGG + chr3.2:37222751-37222770 MS.gene92032:CDS 60.0%
GACAGCGAGGGAGACGAGGA+GGG + chr3.2:37223666-37223685 MS.gene92032:intron 65.0%
GCATGACAGCGAGGGAGACG+AGG + chr3.2:37223662-37223681 MS.gene92032:intron 65.0%
GCCTCGGAGCATGACAGCGA+GGG + chr3.2:37223654-37223673 MS.gene92032:intron 65.0%
TCCCTCGCTGTCATGCTCCG+AGG - chr3.2:37223658-37223677 None:intergenic 65.0%
TGACAGCGAGGGAGACGAGG+AGG + chr3.2:37223665-37223684 MS.gene92032:intron 65.0%
TGCCTCGGAGCATGACAGCG+AGG + chr3.2:37223653-37223672 MS.gene92032:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 37222611 37224353 37222611 ID=MS.gene92032
chr3.2 mRNA 37222611 37224353 37222611 ID=MS.gene92032.t1;Parent=MS.gene92032
chr3.2 exon 37222611 37223348 37222611 ID=MS.gene92032.t1.exon1;Parent=MS.gene92032.t1
chr3.2 CDS 37222611 37223348 37222611 ID=cds.MS.gene92032.t1;Parent=MS.gene92032.t1
chr3.2 exon 37223425 37223522 37223425 ID=MS.gene92032.t1.exon2;Parent=MS.gene92032.t1
chr3.2 CDS 37223425 37223522 37223425 ID=cds.MS.gene92032.t1;Parent=MS.gene92032.t1
chr3.2 exon 37223837 37224353 37223837 ID=MS.gene92032.t1.exon3;Parent=MS.gene92032.t1
chr3.2 CDS 37223837 37224353 37223837 ID=cds.MS.gene92032.t1;Parent=MS.gene92032.t1
Gene Sequence

>MS.gene92032

ATGTATTCTCCAAATCCAAATCCTTCCATATCACGCGCCATCGTTCTAGTTCCCAACGAGCCACAAAATTACTACAACAACACTCTGGCGGGAGAAGCTGCGACATCAAAATCAAAGCAGAAGCTTAAAGATCATAACAAGGGGCTTGTTAGCACTGCGATGGATGGGGATCACCGTCAATGTCGTGAGATGGTCTGGAGAACAATATTGATTTCTAATTTGATTCGCGTGTCGGGTCTGCCAGAAGAAGAGAATAATTGTAAGGTCATAAAAGTTTTGAAAGCTTCATCTTTGATGAACAAGTTGGAAGAAGTGAATAATAGTAGGGTCAGAAATGATTTGATAGCTGCTACTTCGATGAACGATCATCGTTTGTGTCTGTATCATGATAAGAAAATCATTGGTCCAGTTCCTGGTGTTTGTATTGGTGATGTTTTTCTCTATAGAACTGAAATGTGTGTGATTGGTTTACATGGTCAACCACAAGCTGGGATTGATTATTCACATCATAATATGAGTACTAATGGCCAACCCATTGCTACTAGTGTGATTGTGTCTAGTGGATATGATGATGTTGATCAAGGTGACTTTATTACCTATTCTGGCCAAGGTGATAAGAAGAAGAATCAAAAGTTGCAAGGAGGCAATCTTGCTATGGTGACAAGTATGCAATATGAGATTGATGTTAGGGTTATTCGATGTGTTGAGTATGATGGTGTTGTTGCTACTACTGGTAAGGTTTTTGTTTATGGGCTTGTATAAGATAATTAAATGTTGGTGTGAAAATGGTGAATCTGGTTTTCGTGTTTATAAGTTTTTGTTATCGAGGGTTCAAGGGCAACCTAAGATGGGTAGTATGATCCTTGAGGAAGATCGTATGCTTAAGATAGGTCAATTATGTTCTAATCCTATGTATGTTCTTTCTCATAATATTTCCAATGGGAAGGAGAACATTGGTGTGAGTTTATATAATGACATTGATAATGATCAATATCCTATGCAATTCGAGTATCTCCCGGAGGCTGCTTTCCGCAATTCGTGTTGCCTCGGAGCATGACAGCGAGGGAGACGAGGAGGGCGAAAGAGTGTTGTGAATGCGTTGATGGATGCATTTTTTGAAACAAGAATGGCAACATTTTTCCTTATAGTAAAAGTGGGATTCTTCTGAAAGGGAGGTCATTAATTTATGAATGCAGTCCTTTTTGTAGTTGTTCTTTGCATTGTAGAAACCGTGTGACACAAAAAGGTCTTGAACATAGGTTAAAAGTGTTTAGATCTAATGAATCTAGTTGGGGAGTTAGGTCTTTGGACCTTATCCTAGCTGACGCATTTATATGTGAGTTCACCGGACTTGTTTTAACTAGGCAGCAAGTGGAAATTTTAACCATGGATGGTGAACATAACTCATTGATGATATATCCAAGTCGATTTTTCAATAGTCGCACTCAAGAATGGGGTGATTTATCGAGGATAGATGCTAATCATGTGCATCCCTCATATCCCCCTTTAGATTTTGCATTGGATATATCAATGATGAGAAATGTTGCCAACTATATCAGTCATAGCCCAACTCCTAATGTGTTTGTTCAATTAGTTCTCTTTGATCACCATAATTGGAGGTTTCCCCATCTTATGGTATTTGCAATGGAGAACATTCCTCCAATGAGAGAGCTTAGTCTTGATCATGGAAAAGATGCTGGTTTGGATGAGAAATTGACCGGAAAGCTCGCGATATGTAATTGA
Protein sequence

>MS.gene92032.t1

MYSPNPNPSISRAIVLVPNEPQNYYNNTLAGEAATSKSKQKLKDHNKGLVSTAMDGDHRQCREMVWRTILISNLIRVSGLPEEENNCKVIKVLKASSLMNKLEEVNNSRVRNDLIAATSMNDHRLCLYHDKKIIGPVPGVCIGDVFLYRTEMCVIGLHGQPQAGIDYSHHNMSTNGQPIATSVIVSSGYDDVDQGDFITYSGQGDKKKNQKLQGGNLAMVTSMQYEIDVRVIRCVEYDGVVATTGKFLLSRVQGQPKMGSMILEEDRMLKIGQLCSNPINRVTQKGLEHRLKVFRSNESSWGVRSLDLILADAFICEFTGLVLTRQQVEILTMDGEHNSLMIYPSRFFNSRTQEWGDLSRIDANHVHPSYPPLDFALDISMMRNVANYISHSPTPNVFVQLVLFDHHNWRFPHLMVFAMENIPPMRELSLDHGKDAGLDEKLTGKLAICN