Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92341.t1 | XP_024642174.1 | 81.9 | 838 | 100 | 5 | 69 | 858 | 4 | 837 | 0.00E+00 | 1340.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92341.t1 | Q8LPU5 | 52.5 | 775 | 289 | 12 | 125 | 843 | 141 | 892 | 2.9e-218 | 760.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92341.t1 | A0A072U860 | 80.2 | 848 | 102 | 6 | 69 | 858 | 4 | 843 | 0.0e+00 | 1322.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92341.t1 | MTR_6g037900 | 83.451 | 852 | 74 | 7 | 65 | 858 | 1 | 843 | 0.0 | 1345 |
| MS.gene92341.t1 | MTR_6g084890 | 71.395 | 846 | 174 | 7 | 68 | 858 | 3 | 835 | 0.0 | 1222 |
| MS.gene92341.t1 | MTR_5g023660 | 70.937 | 843 | 176 | 8 | 68 | 854 | 3 | 832 | 0.0 | 1180 |
| MS.gene92341.t1 | MTR_7g007090 | 69.158 | 843 | 190 | 8 | 68 | 858 | 3 | 827 | 0.0 | 1177 |
| MS.gene92341.t1 | MTR_5g023660 | 70.819 | 843 | 172 | 9 | 68 | 854 | 3 | 827 | 0.0 | 1175 |
| MS.gene92341.t1 | MTR_4g017850 | 64.988 | 854 | 223 | 9 | 68 | 858 | 3 | 843 | 0.0 | 1115 |
| MS.gene92341.t1 | MTR_6g088715 | 51.207 | 787 | 300 | 13 | 125 | 842 | 146 | 917 | 0.0 | 776 |
| MS.gene92341.t1 | MTR_8g071100 | 45.256 | 780 | 350 | 12 | 125 | 841 | 194 | 959 | 0.0 | 678 |
| MS.gene92341.t1 | MTR_5g066780 | 42.133 | 769 | 284 | 16 | 125 | 842 | 123 | 781 | 6.79e-180 | 538 |
| MS.gene92341.t1 | MTR_5g066830 | 58.421 | 190 | 74 | 1 | 670 | 854 | 1 | 190 | 1.53e-67 | 223 |
| MS.gene92341.t1 | MTR_8g062550 | 32.653 | 441 | 215 | 13 | 180 | 609 | 37 | 406 | 1.10e-53 | 192 |
| MS.gene92341.t1 | MTR_2g046020 | 31.818 | 374 | 176 | 10 | 499 | 825 | 1232 | 1573 | 9.37e-43 | 169 |
| MS.gene92341.t1 | MTR_6g065580 | 31.818 | 374 | 176 | 10 | 499 | 825 | 1206 | 1547 | 1.86e-41 | 165 |
| MS.gene92341.t1 | MTR_3g040810 | 87.500 | 72 | 9 | 0 | 1 | 72 | 1 | 72 | 2.20e-31 | 130 |
| MS.gene92341.t1 | MTR_4g076670 | 70.370 | 81 | 24 | 0 | 1 | 81 | 1 | 81 | 1.55e-30 | 116 |
| MS.gene92341.t1 | MTR_3g040520 | 80.556 | 72 | 14 | 0 | 1 | 72 | 3 | 74 | 9.57e-29 | 122 |
| MS.gene92341.t1 | MTR_7g047650 | 33.108 | 148 | 77 | 5 | 277 | 421 | 1 | 129 | 7.95e-13 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92341.t1 | AT1G69770 | 47.602 | 855 | 350 | 15 | 69 | 849 | 4 | 834 | 0.0 | 743 |
| MS.gene92341.t1 | AT1G80740 | 48.310 | 799 | 338 | 12 | 107 | 845 | 2 | 785 | 0.0 | 741 |
| MS.gene92341.t1 | AT4G19020 | 44.750 | 800 | 371 | 11 | 108 | 849 | 503 | 1289 | 0.0 | 687 |
| MS.gene92341.t1 | AT4G19020 | 44.750 | 800 | 371 | 11 | 108 | 849 | 455 | 1241 | 0.0 | 687 |
| MS.gene92341.t1 | AT4G14140 | 31.818 | 374 | 176 | 10 | 499 | 825 | 1168 | 1509 | 2.06e-43 | 171 |
| MS.gene92341.t1 | AT4G14140 | 31.818 | 374 | 176 | 10 | 499 | 825 | 1168 | 1509 | 2.06e-43 | 171 |
| MS.gene92341.t1 | AT4G14140 | 31.818 | 374 | 176 | 10 | 499 | 825 | 1194 | 1535 | 2.56e-43 | 171 |
| MS.gene92341.t1 | AT4G08990 | 31.551 | 374 | 177 | 10 | 499 | 825 | 1161 | 1502 | 2.27e-42 | 168 |
| MS.gene92341.t1 | AT4G13610 | 32.486 | 354 | 188 | 11 | 499 | 825 | 1070 | 1399 | 6.14e-39 | 157 |
| MS.gene92341.t1 | AT5G49160 | 30.728 | 371 | 184 | 9 | 499 | 825 | 1183 | 1524 | 2.22e-38 | 155 |
| MS.gene92341.t1 | AT5G49160 | 30.728 | 371 | 184 | 9 | 499 | 825 | 1095 | 1436 | 2.39e-38 | 155 |
| MS.gene92341.t1 | AT5G10270 | 76.712 | 73 | 16 | 1 | 1 | 72 | 3 | 75 | 4.11e-25 | 110 |
| MS.gene92341.t1 | AT5G64960 | 77.778 | 72 | 15 | 1 | 2 | 72 | 4 | 75 | 1.43e-24 | 109 |
| MS.gene92341.t1 | AT1G67580 | 59.649 | 57 | 23 | 0 | 15 | 71 | 398 | 454 | 6.09e-12 | 70.1 |
| MS.gene92341.t1 | AT1G67580 | 59.649 | 57 | 23 | 0 | 15 | 71 | 398 | 454 | 6.09e-12 | 70.1 |
Find 191 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCTTGTCAAGGTGTTTC+TGG | 0.071366 | 5.1:+500819 | MS.gene92341:CDS |
| CAAATCGGCGAAGGCACTTA+TGG | 0.185430 | 5.1:+496414 | MS.gene92341:CDS |
| AGACAACTTGGGTACTTAAA+TGG | 0.211840 | 5.1:-503577 | None:intergenic |
| AGCCATTGTCTTTCAACATT+TGG | 0.217136 | 5.1:-502311 | None:intergenic |
| TAAATCAGAGTGGAGTTTAT+TGG | 0.245534 | 5.1:+499314 | MS.gene92341:CDS |
| GCCATTGTCTTTCAACATTT+GGG | 0.251236 | 5.1:-502310 | None:intergenic |
| CATTCTAGCTTGATAATTCA+TGG | 0.269877 | 5.1:-501021 | None:intergenic |
| GTACTGCTTTCTATTCTTAC+TGG | 0.286566 | 5.1:-499246 | None:intergenic |
| ATGATGGCTGCAGGGTCTTA+TGG | 0.291327 | 5.1:+501047 | MS.gene92341:CDS |
| TGCAAGGCTACAGGGATTTC+CGG | 0.292513 | 5.1:+502968 | MS.gene92341:CDS |
| ACATTAGGTTTGGCTATTCT+AGG | 0.293225 | 5.1:+503523 | MS.gene92341:CDS |
| TGCTTTGGAGCTTCTAAATC+TGG | 0.296220 | 5.1:+499375 | MS.gene92341:CDS |
| GAAACGGCTGCTATTTGTTT+AGG | 0.296537 | 5.1:-498064 | None:intergenic |
| AAATCATACCACTTCTCTTT+AGG | 0.308647 | 5.1:-501673 | None:intergenic |
| CTTATGGCCTTCCCCAATTT+CGG | 0.310436 | 5.1:+501063 | MS.gene92341:CDS |
| TGGAAATGCGGTTGCTGTTC+CGG | 0.310761 | 5.1:+503480 | MS.gene92341:CDS |
| CTTGCCCTTGGATACACATT+AGG | 0.314307 | 5.1:+503508 | MS.gene92341:CDS |
| TCCGAACAAAGCCAAGAAAA+AGG | 0.318064 | 5.1:+499906 | MS.gene92341:CDS |
| TATGCCATGTCATTTGTTAA+AGG | 0.326992 | 5.1:+502444 | MS.gene92341:CDS |
| GAAAGACAATGGCTAAAATC+TGG | 0.331186 | 5.1:+502320 | MS.gene92341:CDS |
| CTAAGAACTCTGTCTTGTTC+CGG | 0.338154 | 5.1:-502933 | None:intergenic |
| TCAAGATCTGTAATTCCTAT+TGG | 0.338902 | 5.1:+501270 | MS.gene92341:CDS |
| ACGTTCTCCAATGTTTCTAA+TGG | 0.340393 | 5.1:+499122 | MS.gene92341:CDS |
| CAGACGAAGCTCTGTCAAAA+TGG | 0.344828 | 5.1:+498236 | MS.gene92341:CDS |
| ACAAATGACATGGCATAATC+TGG | 0.347629 | 5.1:-502438 | None:intergenic |
| CTTTATAGGGGTCTAACTTC+AGG | 0.348142 | 5.1:+502243 | MS.gene92341:intron |
| TTCAACTTGAACATCTTGTT+TGG | 0.350986 | 5.1:-498113 | None:intergenic |
| TCTACAATTTCCAGTGAATC+TGG | 0.352267 | 5.1:+499270 | MS.gene92341:CDS |
| TCGTCTTTATTTAATTTCAA+TGG | 0.353998 | 5.1:-502105 | None:intergenic |
| ATGCCATGTCATTTGTTAAA+GGG | 0.367045 | 5.1:+502445 | MS.gene92341:CDS |
| TGGCTCCCATGTATCGTAAC+TGG | 0.367392 | 5.1:-500425 | None:intergenic |
| CAACGATAATGCTTATGTTC+AGG | 0.376205 | 5.1:+498443 | MS.gene92341:CDS |
| TTTGGTTACTCTCTTTCGTT+TGG | 0.378274 | 5.1:-498161 | None:intergenic |
| AGAGCTTCGTCTGCAGGAAT+AGG | 0.379729 | 5.1:-498226 | None:intergenic |
| GGTATCAACCGCTGATTGTT+TGG | 0.382568 | 5.1:-498179 | None:intergenic |
| CATGAATTATCAAGCTAGAA+TGG | 0.391346 | 5.1:+501022 | MS.gene92341:CDS |
| ATGTCCACGGGACTTTGCTT+TGG | 0.402168 | 5.1:+499360 | MS.gene92341:CDS |
| ATCTGATTCTGAGGTGTTTG+AGG | 0.402209 | 5.1:+499852 | MS.gene92341:CDS |
| TAAGAACTCTGTCTTGTTCC+GGG | 0.405750 | 5.1:-502932 | None:intergenic |
| CAACTAGCAATGGCAGCAGC+TGG | 0.409192 | 5.1:+496324 | None:intergenic |
| CCGGACTGCTATAAACTTTG+CGG | 0.410014 | 5.1:+502987 | MS.gene92341:CDS |
| GCTAAAATCTGGGAAGCCTT+TGG | 0.412017 | 5.1:+502331 | MS.gene92341:CDS |
| TACCGAAAAGCAAAAGTTGA+TGG | 0.420755 | 5.1:+498405 | MS.gene92341:CDS |
| TCAATAATAACCTGATTATG+AGG | 0.425804 | 5.1:-502632 | None:intergenic |
| CCTTGGATACACATTAGGTT+TGG | 0.425858 | 5.1:+503513 | MS.gene92341:CDS |
| TTCACCGGTTTCGATCTCTT+TGG | 0.427011 | 5.1:-496539 | None:intergenic |
| TTTGACAGAGCTTCGTCTGC+AGG | 0.430503 | 5.1:-498232 | None:intergenic |
| TCGAGAAAATGCAAGGCTAC+AGG | 0.432318 | 5.1:+502959 | MS.gene92341:CDS |
| TTCCTTACTTGAAAGCCAAT+AGG | 0.432768 | 5.1:-501285 | None:intergenic |
| GATTACCTCAAGCCTTCAAT+TGG | 0.435386 | 5.1:-500445 | None:intergenic |
| GTTCCGGTAGCTCTTGCCCT+TGG | 0.438118 | 5.1:+503496 | MS.gene92341:CDS |
| GAGAGCAGAACCTCATAATC+AGG | 0.444511 | 5.1:+502622 | MS.gene92341:CDS |
| CCCACTCTTTAAGCAAACTT+AGG | 0.450591 | 5.1:-499720 | None:intergenic |
| TTTGAGAAACTCGAACAAAT+CGG | 0.450626 | 5.1:+496399 | MS.gene92341:CDS |
| AAATCGGCGAAGGCACTTAT+GGG | 0.452605 | 5.1:+496415 | MS.gene92341:CDS |
| GACAACTTGGGTACTTAAAT+GGG | 0.454068 | 5.1:-503576 | None:intergenic |
| ACAGGCCATTTGGTCGACTT+TGG | 0.454391 | 5.1:+502570 | MS.gene92341:intron |
| CTCTGTCAAAATGGCCTCAC+CGG | 0.455285 | 5.1:+498245 | MS.gene92341:CDS |
| CAATGCAGGTATACTCAAGT+TGG | 0.460036 | 5.1:+503460 | MS.gene92341:intron |
| CAACAGTACATACCTTGCTT+TGG | 0.464340 | 5.1:-503015 | None:intergenic |
| ATGATTCCAGTTACGATACA+TGG | 0.465370 | 5.1:+500419 | MS.gene92341:CDS |
| ACACAACATACGTGTGTTTG+AGG | 0.467072 | 5.1:-499569 | None:intergenic |
| ATTCATACCATTAGAAACAT+TGG | 0.467339 | 5.1:-499129 | None:intergenic |
| TGTTAAAGATGAAAACCCTC+TGG | 0.470129 | 5.1:+498873 | MS.gene92341:CDS |
| TTGGATTTGTACGCTGGTTG+TGG | 0.470529 | 5.1:+499333 | MS.gene92341:CDS |
| AAAGAAGGGGCAAGACAACT+TGG | 0.472467 | 5.1:-503589 | None:intergenic |
| ATGAATTATCAAGCTAGAAT+GGG | 0.473314 | 5.1:+501023 | MS.gene92341:CDS |
| CTTCTTGTGTGGAATGTGGC+AGG | 0.481865 | 5.1:-502139 | None:intergenic |
| TAATGGTAACAGTGACTGTA+AGG | 0.482488 | 5.1:+500587 | MS.gene92341:intron |
| TTGCGCTTTGTAGTGGTTCG+AGG | 0.485360 | 5.1:+498352 | MS.gene92341:intron |
| TTTATAGGGGTCTAACTTCA+GGG | 0.486332 | 5.1:+502244 | MS.gene92341:intron |
| GCCATTTGGTCGACTTTGGT+GGG | 0.487889 | 5.1:+502574 | MS.gene92341:CDS |
| GACATTAGATCCAGATTCAC+TGG | 0.489714 | 5.1:-499280 | None:intergenic |
| TCATCAACTGTATCTAAAGA+AGG | 0.490255 | 5.1:-503604 | None:intergenic |
| TGCTTGCAGTCAAGTTTACA+TGG | 0.493561 | 5.1:+496516 | MS.gene92341:intron |
| TCAACCTCGTGAGTTGGTAA+TGG | 0.495693 | 5.1:-501246 | None:intergenic |
| AGAATGGGAATGATGGCTGC+AGG | 0.497284 | 5.1:+501038 | MS.gene92341:CDS |
| GATACATGGGAGCCAATTGA+AGG | 0.497307 | 5.1:+500433 | MS.gene92341:CDS |
| CCTAAGTTTGCTTAAAGAGT+GGG | 0.497638 | 5.1:+499720 | MS.gene92341:CDS |
| TATCCAAGGGCAAGAGCTAC+CGG | 0.502430 | 5.1:-503499 | None:intergenic |
| CTTGTGCTCTGTAAAACCAC+TGG | 0.503316 | 5.1:-498681 | None:intergenic |
| GGCCATTTGGTCGACTTTGG+TGG | 0.503320 | 5.1:+502573 | MS.gene92341:intron |
| CATCAACTGTATCTAAAGAA+GGG | 0.504781 | 5.1:-503603 | None:intergenic |
| AGTACATACCTTGCTTTGGT+AGG | 0.508546 | 5.1:-503011 | None:intergenic |
| GGTCAAGCTGATGTTATTTG+TGG | 0.509662 | 5.1:+500792 | MS.gene92341:CDS |
| CTTGAGAGCAACAATTTCAC+CGG | 0.511084 | 5.1:-496554 | None:intergenic |
| CTCAAAGCAATCGACGCTAC+GGG | 0.511892 | 5.1:-496382 | None:intergenic |
| GTATGGATAATGAAAGAGAA+GGG | 0.512036 | 5.1:+496584 | MS.gene92341:CDS |
| GGGCGGTAAAGAACTGTTGT+TGG | 0.516330 | 5.1:-498660 | None:intergenic |
| AAAGACAATGGCTAAAATCT+GGG | 0.517011 | 5.1:+502321 | MS.gene92341:CDS |
| AAGCTACTTGCAGTCTGCTA+TGG | 0.517571 | 5.1:+499880 | MS.gene92341:CDS |
| TGCGAAGAGTTTGTATTGCA+TGG | 0.518127 | 5.1:+499751 | MS.gene92341:CDS |
| AATCCTTCAGGTGCAGTGGA+AGG | 0.519590 | 5.1:+500393 | MS.gene92341:intron |
| AACTAGCAATGGCAGCAGCT+GGG | 0.520780 | 5.1:+496325 | None:intergenic |
| CAAGCTGATGTTATTTGTGG+TGG | 0.521120 | 5.1:+500795 | MS.gene92341:CDS |
| GTTACTCTCTTTCGTTTGGA+AGG | 0.523298 | 5.1:-498157 | None:intergenic |
| TGCTCTCAAGCAAATACGTA+TGG | 0.526233 | 5.1:+496567 | MS.gene92341:CDS |
| GAAGGGCAATAAAGTGGAAT+GGG | 0.528703 | 5.1:+502286 | MS.gene92341:CDS |
| TCCCACCAAAGTCGACCAAA+TGG | 0.528934 | 5.1:-502575 | None:intergenic |
| GAAGGAAGAAGCGAAACAAT+GGG | 0.528938 | 5.1:-498139 | None:intergenic |
| ATCCTTCAGGTGCAGTGGAA+GGG | 0.529559 | 5.1:+500394 | MS.gene92341:intron |
| AGCCATGATGAAAGGAGCTA+CGG | 0.530405 | 5.1:+501614 | MS.gene92341:CDS |
| AGCCGTTTCAAAACCAAAGA+AGG | 0.530824 | 5.1:+498078 | MS.gene92341:CDS |
| GCCATGATGAAAGGAGCTAC+GGG | 0.531899 | 5.1:+501615 | MS.gene92341:CDS |
| TCAAGCTAGAATGGGAATGA+TGG | 0.534324 | 5.1:+501031 | MS.gene92341:CDS |
| TCTCAAAGCAATCGACGCTA+CGG | 0.534411 | 5.1:-496383 | None:intergenic |
| ATGGCCAAAGAGATCGAAAC+CGG | 0.536311 | 5.1:+496535 | MS.gene92341:CDS |
| TCAAAATATAACTCACAAGA+AGG | 0.537737 | 5.1:-499074 | None:intergenic |
| CGAGAAAATGCAAGGCTACA+GGG | 0.538395 | 5.1:+502960 | MS.gene92341:CDS |
| TTCTCCATGAGTACGTATCT+CGG | 0.538527 | 5.1:-500933 | None:intergenic |
| TGAGTTGGTAATGGAAATGC+AGG | 0.540523 | 5.1:-501237 | None:intergenic |
| TCCTAAGTTTGCTTAAAGAG+TGG | 0.542746 | 5.1:+499719 | MS.gene92341:CDS |
| ACCCTCTGGGTTGTATACTT+AGG | 0.543791 | 5.1:+498887 | MS.gene92341:CDS |
| CCGCAAAGTTTATAGCAGTC+CGG | 0.544129 | 5.1:-502987 | None:intergenic |
| GTGGTGTGACTCACAGGAAG+AGG | 0.545184 | 5.1:-500657 | None:intergenic |
| CCAAACCTAATGTGTATCCA+AGG | 0.546084 | 5.1:-503513 | None:intergenic |
| TGAAGGGCAATAAAGTGGAA+TGG | 0.548725 | 5.1:+502285 | MS.gene92341:CDS |
| CTTGATTCAACCTCGTGAGT+TGG | 0.550035 | 5.1:-501252 | None:intergenic |
| GTACATACCTTGCTTTGGTA+GGG | 0.555275 | 5.1:-503010 | None:intergenic |
| ATCTGGCATTAAACTAGTCA+CGG | 0.555721 | 5.1:+499392 | MS.gene92341:CDS |
| CGTATGGATAATGAAAGAGA+AGG | 0.557605 | 5.1:+496583 | MS.gene92341:CDS |
| TGTTGTAACAAAATCTCTCA+AGG | 0.559679 | 5.1:-500613 | None:intergenic |
| CTGCCACATTCCACACAAGA+AGG | 0.563625 | 5.1:+502140 | MS.gene92341:CDS |
| ATGACGAGAGCCATGATGAA+AGG | 0.566976 | 5.1:+501606 | MS.gene92341:CDS |
| CCCCGTAGCTCCTTTCATCA+TGG | 0.568068 | 5.1:-501616 | None:intergenic |
| GATTGTGAAGGGCAATAAAG+TGG | 0.568397 | 5.1:+502280 | MS.gene92341:CDS |
| GGAAGGAAGAAGCGAAACAA+TGG | 0.569759 | 5.1:-498140 | None:intergenic |
| TTTCTAGGTTCTTCTCCACC+CGG | 0.574089 | 5.1:+502914 | MS.gene92341:intron |
| GTTTGCTTAAAGAGTGGGCA+AGG | 0.576424 | 5.1:+499725 | MS.gene92341:CDS |
| GGAGCTACGGGGCTTCTCCT+CGG | 0.578403 | 5.1:+501627 | MS.gene92341:CDS |
| TCATATTCTGATATGAATGA+TGG | 0.580549 | 5.1:+499784 | MS.gene92341:CDS |
| CTCACTCGTTATTCGAGTAC+CGG | 0.580986 | 5.1:-498264 | None:intergenic |
| GGTTGTGGAGCTATGTCCAC+GGG | 0.581227 | 5.1:+499348 | MS.gene92341:CDS |
| AGTTTATTGGATTTGTACGC+TGG | 0.590161 | 5.1:+499327 | MS.gene92341:CDS |
| TGTGGTGGACCACCTTGTCA+AGG | 0.591011 | 5.1:+500810 | MS.gene92341:CDS |
| TATATCCGCCTAAAGAGAAG+TGG | 0.592001 | 5.1:+501665 | MS.gene92341:CDS |
| ATGAATGATGGTGAAGAAGC+TGG | 0.594514 | 5.1:+499796 | MS.gene92341:CDS |
| TCCAACAAAACAGATTCTGT+CGG | 0.594622 | 5.1:-498200 | None:intergenic |
| AGAAGCTAATGATGAAGTTG+TGG | 0.594844 | 5.1:+499822 | MS.gene92341:CDS |
| AAAACGCGCATCCGAAATTG+GGG | 0.595504 | 5.1:-501074 | None:intergenic |
| TGGTTGTGGAGCTATGTCCA+CGG | 0.596354 | 5.1:+499347 | MS.gene92341:CDS |
| ATCAACTGTATCTAAAGAAG+GGG | 0.599017 | 5.1:-503602 | None:intergenic |
| TCATACCACTTCTCTTTAGG+CGG | 0.601281 | 5.1:-501670 | None:intergenic |
| TTAGCATTCGAGAAAATGCA+AGG | 0.604467 | 5.1:+502952 | MS.gene92341:CDS |
| CGCGCATCCGAAATTGGGGA+AGG | 0.604603 | 5.1:-501070 | None:intergenic |
| GAATGGGAATGATGGCTGCA+GGG | 0.605520 | 5.1:+501039 | MS.gene92341:CDS |
| GACTATGAAAGCGCACCAAG+TGG | 0.605628 | 5.1:+502063 | MS.gene92341:CDS |
| GAAGCCGAGATACGTACTCA+TGG | 0.607530 | 5.1:+500929 | MS.gene92341:CDS |
| GGGAGCCAATTGAAGGCTTG+AGG | 0.607663 | 5.1:+500440 | MS.gene92341:CDS |
| GGATGATTCATCTGATTCTG+AGG | 0.610672 | 5.1:+499843 | MS.gene92341:CDS |
| ACATTGGAGAACGTAAGATA+AGG | 0.612116 | 5.1:-499113 | None:intergenic |
| TCTAACTTCAGGGACTTGAA+AGG | 0.616108 | 5.1:+502254 | MS.gene92341:CDS |
| TCCTAAGTATACAACCCAGA+GGG | 0.616856 | 5.1:-498888 | None:intergenic |
| AGATATGGAACCTTCTTGTG+TGG | 0.618250 | 5.1:-502150 | None:intergenic |
| CAAACCTAATGTGTATCCAA+GGG | 0.619120 | 5.1:-503512 | None:intergenic |
| ACCCAAATGTTGAAAGACAA+TGG | 0.621165 | 5.1:+502309 | MS.gene92341:CDS |
| TTGTGCTCTGTAAAACCACT+GGG | 0.625437 | 5.1:-498680 | None:intergenic |
| TATTTCTGAAATTCTGACCG+AGG | 0.626077 | 5.1:-501644 | None:intergenic |
| AGTGAATCTGGATCTAATGT+CGG | 0.626570 | 5.1:+499282 | MS.gene92341:CDS |
| AAGAAGGGGCAAGACAACTT+GGG | 0.629993 | 5.1:-503588 | None:intergenic |
| TGATTCCAGTTACGATACAT+GGG | 0.631111 | 5.1:+500420 | MS.gene92341:CDS |
| ATACGTACTCATGGAGAATG+TGG | 0.633406 | 5.1:+500938 | MS.gene92341:CDS |
| AGCTCCAAAGCAAAGTCCCG+TGG | 0.633503 | 5.1:-499364 | None:intergenic |
| AGATTCACTGGAAATTGTAG+AGG | 0.636580 | 5.1:-499268 | None:intergenic |
| GTTAAAGATGAAAACCCTCT+GGG | 0.641620 | 5.1:+498874 | MS.gene92341:CDS |
| TTAAATGGGAGAGTTGTCAA+AGG | 0.642072 | 5.1:-503562 | None:intergenic |
| CTTGAAAGGTGTGATTGTGA+AGG | 0.643046 | 5.1:+502268 | MS.gene92341:CDS |
| TTGAAAGGTGTGATTGTGAA+GGG | 0.646065 | 5.1:+502269 | MS.gene92341:CDS |
| GAACTCTGTCTTGTTCCGGG+TGG | 0.647742 | 5.1:-502929 | None:intergenic |
| TATGGATAATGAAAGAGAAG+GGG | 0.649789 | 5.1:+496585 | MS.gene92341:CDS |
| TGATCATATAGCATTCCACT+TGG | 0.652584 | 5.1:-502078 | None:intergenic |
| TCGTTATTCGAGTACCGGTG+AGG | 0.654914 | 5.1:-498259 | None:intergenic |
| GTTTAGGTGATTGACGAATG+CGG | 0.655585 | 5.1:-498048 | None:intergenic |
| ACGCTACGGGAACCACGCGA+AGG | 0.656742 | 5.1:-496369 | None:intergenic |
| GATAATTCATGGCTACAAGA+CGG | 0.657208 | 5.1:-501010 | None:intergenic |
| AAACTCGAACAAATCGGCGA+AGG | 0.657326 | 5.1:+496405 | MS.gene92341:CDS |
| AACAGTTCTTTACCGCCCAG+TGG | 0.658721 | 5.1:+498665 | MS.gene92341:CDS |
| GAACCTTCTTGTGTGGAATG+TGG | 0.658764 | 5.1:-502143 | None:intergenic |
| GGAGAACGTAAGATAAGGCA+CGG | 0.660211 | 5.1:-499108 | None:intergenic |
| TTAATTGATTCACCTTCGCG+TGG | 0.661011 | 5.1:+496357 | MS.gene92341:CDS |
| TTTGCGGCCCTACCAAAGCA+AGG | 0.665769 | 5.1:+503003 | MS.gene92341:CDS |
| GAGAGTAACCAAACAATCAG+CGG | 0.670551 | 5.1:+498171 | MS.gene92341:CDS |
| ATTTCCATTACCAACTCACG+AGG | 0.675556 | 5.1:+501242 | MS.gene92341:CDS |
| AGTCCCTTTAACAAATGACA+TGG | 0.677607 | 5.1:-502448 | None:intergenic |
| CCAGAAACACCTTGACAAGG+TGG | 0.678616 | 5.1:-500819 | None:intergenic |
| GTATACTCAAGTTGGAAATG+CGG | 0.682221 | 5.1:+503468 | MS.gene92341:CDS |
| AAACCAGAAACACCTTGACA+AGG | 0.696680 | 5.1:-500822 | None:intergenic |
| TTCCTAAGTATACAACCCAG+AGG | 0.723332 | 5.1:-498889 | None:intergenic |
| CCATGATGAAAGGAGCTACG+GGG | 0.724816 | 5.1:+501616 | MS.gene92341:CDS |
| GTGCTGCTGATAAATCAGAG+TGG | 0.738745 | 5.1:+499304 | MS.gene92341:CDS |
| TGCTCTGTAAAACCACTGGG+CGG | 0.740254 | 5.1:-498677 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 496333 | 503645 | 496333 | ID=MS.gene92341 |
| chr5.1 | mRNA | 496333 | 503645 | 496333 | ID=MS.gene92341.t1;Parent=MS.gene92341 |
| chr5.1 | exon | 496333 | 496436 | 496333 | ID=MS.gene92341.t1.exon1;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 496333 | 496436 | 496333 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 496525 | 496606 | 496525 | ID=MS.gene92341.t1.exon2;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 496525 | 496606 | 496525 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 498028 | 498281 | 498028 | ID=MS.gene92341.t1.exon3;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 498028 | 498281 | 498028 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 498365 | 498464 | 498365 | ID=MS.gene92341.t1.exon4;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 498365 | 498464 | 498365 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 498601 | 498708 | 498601 | ID=MS.gene92341.t1.exon5;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 498601 | 498708 | 498601 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 498820 | 498939 | 498820 | ID=MS.gene92341.t1.exon6;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 498820 | 498939 | 498820 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 499038 | 499143 | 499038 | ID=MS.gene92341.t1.exon7;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 499038 | 499143 | 499038 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 499244 | 499413 | 499244 | ID=MS.gene92341.t1.exon8;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 499244 | 499413 | 499244 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 499515 | 499580 | 499515 | ID=MS.gene92341.t1.exon9;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 499515 | 499580 | 499515 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 499694 | 499963 | 499694 | ID=MS.gene92341.t1.exon10;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 499694 | 499963 | 499694 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 500403 | 500461 | 500403 | ID=MS.gene92341.t1.exon11;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 500403 | 500461 | 500403 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 500599 | 500665 | 500599 | ID=MS.gene92341.t1.exon12;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 500599 | 500665 | 500599 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 500792 | 501091 | 500792 | ID=MS.gene92341.t1.exon13;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 500792 | 501091 | 500792 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 501231 | 501299 | 501231 | ID=MS.gene92341.t1.exon14;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 501231 | 501299 | 501231 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 501599 | 501686 | 501599 | ID=MS.gene92341.t1.exon15;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 501599 | 501686 | 501599 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 502043 | 502161 | 502043 | ID=MS.gene92341.t1.exon16;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 502043 | 502161 | 502043 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 502251 | 502352 | 502251 | ID=MS.gene92341.t1.exon17;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 502251 | 502352 | 502251 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 502435 | 502478 | 502435 | ID=MS.gene92341.t1.exon18;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 502435 | 502478 | 502435 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 502574 | 502643 | 502574 | ID=MS.gene92341.t1.exon19;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 502574 | 502643 | 502574 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 502921 | 503024 | 502921 | ID=MS.gene92341.t1.exon20;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 502921 | 503024 | 502921 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| chr5.1 | exon | 503468 | 503645 | 503468 | ID=MS.gene92341.t1.exon21;Parent=MS.gene92341.t1 |
| chr5.1 | CDS | 503468 | 503645 | 503468 | ID=cds.MS.gene92341.t1;Parent=MS.gene92341.t1 |
| Gene Sequence |
| Protein sequence |
