AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92359

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92359.t1 MTR_5g005110 90.090 333 18 4 1 320 1 331 0.0 600
MS.gene92359.t1 MTR_5g005110 88.679 265 15 4 1 252 1 263 1.13e-165 462
MS.gene92359.t1 MTR_1g106460 49.706 340 122 12 1 303 1 328 5.73e-85 259
MS.gene92359.t1 MTR_1g106470 48.243 313 124 9 1 302 1 286 2.19e-77 238
MS.gene92359.t1 MTR_3g103030 51.142 219 87 8 93 301 111 319 3.42e-57 187
MS.gene92359.t1 MTR_1g106460 43.056 288 115 12 1 251 1 276 1.52e-47 161
MS.gene92359.t1 MTR_1g018090 58.491 159 53 4 133 288 201 349 2.04e-44 155
MS.gene92359.t1 MTR_1g106470 40.909 264 119 8 1 254 1 237 1.86e-43 149
MS.gene92359.t1 MTR_1g106480 56.081 148 30 4 187 302 12 156 1.13e-39 137
MS.gene92359.t1 MTR_5g079500 62.500 72 27 0 210 281 215 286 1.23e-25 104
MS.gene92359.t1 MTR_1g018090 49.206 126 51 4 133 255 201 316 5.42e-19 86.3
MS.gene92359.t1 MTR_4g131160 60.000 65 26 0 212 276 195 259 8.97e-19 86.7
MS.gene92359.t1 MTR_4g131160 60.000 65 26 0 212 276 198 262 9.50e-19 86.7
MS.gene92359.t1 MTR_8g093500 66.071 56 19 0 222 277 123 178 2.00e-18 84.0
MS.gene92359.t1 MTR_3g101810 61.111 54 21 0 222 275 180 233 8.68e-17 79.3
MS.gene92359.t1 MTR_7g069590 60.000 55 22 0 220 274 33 87 9.69e-17 76.6
MS.gene92359.t1 MTR_1g080890 58.462 65 27 0 212 276 192 256 1.12e-16 80.1
MS.gene92359.t1 MTR_1g019240 39.604 101 54 1 197 290 62 162 1.42e-15 75.9
MS.gene92359.t1 MTR_3g449770 45.570 79 36 2 206 284 304 375 7.33e-14 72.4
MS.gene92359.t1 MTR_1g084980 46.970 66 35 0 211 276 460 525 1.22e-13 72.0
MS.gene92359.t1 MTR_5g017040 43.939 66 37 0 224 289 116 181 7.11e-13 68.9
MS.gene92359.t1 MTR_4g033295 52.239 67 31 1 212 277 117 183 1.62e-12 65.5
MS.gene92359.t1 MTR_4g033295 54.545 66 29 1 212 276 117 182 1.70e-12 65.9
MS.gene92359.t1 MTR_1g069155 45.455 66 36 0 211 276 315 380 1.98e-12 68.2
MS.gene92359.t1 MTR_5g048860 33.951 162 87 4 123 276 280 429 3.47e-12 67.4
MS.gene92359.t1 MTR_7g053410 51.562 64 30 1 214 276 318 381 9.53e-12 65.9
MS.gene92359.t1 MTR_7g099540 45.588 68 36 1 209 276 249 315 1.09e-11 65.9
MS.gene92359.t1 MTR_7g053410 51.562 64 30 1 214 276 318 381 1.10e-11 65.9
MS.gene92359.t1 MTR_8g062240 50.000 64 31 1 214 276 152 215 1.21e-11 64.7
MS.gene92359.t1 MTR_8g012290 51.562 64 30 1 214 276 292 355 1.24e-11 65.5
MS.gene92359.t1 MTR_8g012290 51.562 64 30 1 214 276 292 355 1.30e-11 65.5
MS.gene92359.t1 MTR_3g116770 42.708 96 46 3 182 276 168 255 2.13e-11 64.7
MS.gene92359.t1 MTR_3g116770 42.708 96 46 3 182 276 168 255 2.30e-11 64.7
MS.gene92359.t1 MTR_8g036235 48.485 66 33 1 212 276 315 380 2.53e-11 64.3
MS.gene92359.t1 MTR_4g070320 32.520 123 65 2 172 276 85 207 2.58e-11 64.3
MS.gene92359.t1 MTR_8g065740 42.553 94 35 2 202 276 276 369 2.65e-11 64.7
MS.gene92359.t1 MTR_1g116790 50.000 68 33 1 212 278 264 331 8.19e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92359.t1 AT2G14760 41.212 330 142 12 10 299 6 323 2.14e-61 199
MS.gene92359.t1 AT4G33880 40.407 344 146 11 10 295 6 348 9.05e-58 190
MS.gene92359.t1 AT2G14760 40.407 344 138 14 10 299 6 336 1.36e-57 189
MS.gene92359.t1 AT1G27740 67.544 114 36 1 184 296 137 250 1.45e-47 160
MS.gene92359.t1 AT5G43175 54.140 157 53 5 149 296 69 215 5.81e-45 153
MS.gene92359.t1 AT5G37800 70.000 60 18 0 222 281 210 269 2.04e-24 101
MS.gene92359.t1 AT1G66470 71.667 60 17 0 222 281 204 263 2.22e-24 100
MS.gene92359.t1 AT2G24260 63.077 65 24 0 212 276 34 98 3.15e-21 91.3
MS.gene92359.t1 AT2G24260 63.077 65 24 0 212 276 137 201 1.97e-20 90.9
MS.gene92359.t1 AT4G30980 63.077 65 24 0 212 276 129 193 2.13e-20 90.1
MS.gene92359.t1 AT4G30980 63.077 65 24 0 212 276 177 241 4.08e-20 90.1
MS.gene92359.t1 AT5G58010 58.462 65 27 0 212 276 98 162 1.76e-18 84.3
MS.gene92359.t1 AT5G01305 57.377 61 26 0 212 272 36 96 1.80e-17 79.0
MS.gene92359.t1 AT3G21330 57.143 56 24 0 220 275 276 331 4.49e-16 78.6
MS.gene92359.t1 AT4G00120 51.852 54 26 0 222 275 121 174 3.31e-15 73.6
MS.gene92359.t1 AT2G46970 32.520 123 67 4 196 302 166 288 2.04e-13 70.9
MS.gene92359.t1 AT2G46970 32.520 123 67 4 196 302 197 319 2.63e-13 70.5
MS.gene92359.t1 AT2G46970 32.520 123 67 4 196 302 175 297 2.64e-13 70.5
MS.gene92359.t1 AT2G20180 45.679 81 43 1 196 276 191 270 4.79e-13 69.7
MS.gene92359.t1 AT2G20180 45.679 81 43 1 196 276 191 270 4.79e-13 69.7
MS.gene92359.t1 AT2G20180 45.679 81 43 1 196 276 191 270 4.79e-13 69.7
MS.gene92359.t1 AT2G20180 45.679 81 43 1 196 276 262 341 5.38e-13 69.7
MS.gene92359.t1 AT2G20180 45.679 81 43 1 196 276 262 341 5.38e-13 69.7
MS.gene92359.t1 AT2G20180 45.679 81 43 1 196 276 262 341 5.38e-13 69.7
MS.gene92359.t1 AT2G20180 45.679 81 43 1 196 276 262 341 5.38e-13 69.7
MS.gene92359.t1 AT1G09530 41.463 82 44 1 208 285 328 409 6.07e-13 69.7
MS.gene92359.t1 AT1G09530 41.463 82 44 1 208 285 328 409 6.07e-13 69.7
MS.gene92359.t1 AT1G09530 41.463 82 44 1 208 285 328 409 6.07e-13 69.7
MS.gene92359.t1 AT1G09530 41.463 82 44 1 208 285 328 409 6.07e-13 69.7
MS.gene92359.t1 AT1G09530 41.463 82 44 1 208 285 328 409 6.07e-13 69.7
MS.gene92359.t1 AT1G09530 41.463 82 44 1 208 285 328 409 6.07e-13 69.7
MS.gene92359.t1 AT5G50915 46.753 77 39 2 214 289 137 212 1.42e-12 67.4
MS.gene92359.t1 AT5G50915 46.753 77 39 2 214 289 137 212 1.42e-12 67.4
MS.gene92359.t1 AT5G50915 46.753 77 39 2 214 289 137 212 1.71e-12 67.0
MS.gene92359.t1 AT5G50915 46.753 77 39 2 214 289 137 212 1.71e-12 67.0
MS.gene92359.t1 AT2G20180 45.570 79 42 1 196 274 262 339 1.94e-12 67.4
MS.gene92359.t1 AT4G36930 38.889 90 53 2 220 307 198 287 1.95e-12 67.8
MS.gene92359.t1 AT4G00050 37.607 117 56 4 168 280 69 172 3.71e-12 66.2
MS.gene92359.t1 AT1G73830 36.806 144 74 6 142 276 76 211 4.47e-12 65.5
MS.gene92359.t1 AT1G73830 36.806 144 74 6 142 276 76 211 4.61e-12 65.5
MS.gene92359.t1 AT1G59640 43.478 92 44 3 214 304 137 221 5.08e-12 65.5
MS.gene92359.t1 AT1G25330 53.125 64 29 1 214 276 105 168 5.29e-12 64.7
MS.gene92359.t1 AT4G00050 37.607 117 56 4 168 280 171 274 5.65e-12 66.2
MS.gene92359.t1 AT4G00050 37.607 117 56 4 168 280 171 274 6.14e-12 66.2
MS.gene92359.t1 AT4G09180 50.000 68 33 1 212 278 183 250 9.14e-12 64.7
MS.gene92359.t1 AT1G59640 43.011 93 45 3 214 305 137 222 1.34e-11 65.1
MS.gene92359.t1 AT1G35460 51.515 66 31 1 212 276 180 245 1.36e-11 64.3
MS.gene92359.t1 AT3G07340 51.562 64 30 1 214 276 259 322 1.68e-11 65.1
MS.gene92359.t1 AT3G07340 51.562 64 30 1 214 276 259 322 1.91e-11 65.1
MS.gene92359.t1 AT5G48560 51.562 64 30 1 214 276 302 365 1.98e-11 65.1
MS.gene92359.t1 AT5G48560 51.562 64 30 1 214 276 302 365 2.05e-11 65.1
MS.gene92359.t1 AT1G51140 50.769 65 31 1 212 275 303 367 2.12e-11 64.7
MS.gene92359.t1 AT1G26260 51.562 64 30 1 214 276 173 236 2.38e-11 64.3
MS.gene92359.t1 AT1G26260 51.562 64 30 1 214 276 224 287 2.74e-11 64.3
MS.gene92359.t1 AT1G26260 51.562 64 30 1 214 276 224 287 2.74e-11 64.3
MS.gene92359.t1 AT1G26260 51.562 64 30 1 214 276 224 287 2.74e-11 64.3
MS.gene92359.t1 AT1G26260 51.562 64 30 1 214 276 224 287 2.74e-11 64.3
MS.gene92359.t1 AT1G26260 51.562 64 30 1 214 276 202 265 2.76e-11 64.3
MS.gene92359.t1 AT4G34530 50.000 64 31 1 214 276 173 236 3.99e-11 63.5
MS.gene92359.t1 AT5G67110 50.980 51 25 0 226 276 100 150 4.08e-11 62.0
MS.gene92359.t1 AT4G28790 39.326 89 53 1 202 290 260 347 4.47e-11 63.9
MS.gene92359.t1 AT2G14760 29.502 261 130 13 10 229 6 253 8.84e-11 62.0
MS.gene92359.t1 AT1G68920 50.000 64 31 1 214 276 303 366 9.79e-11 62.8
MS.gene92359.t1 AT1G68920 50.000 64 31 1 214 276 304 367 9.81e-11 62.8
MS.gene92359.t1 AT1G68920 50.000 64 31 1 214 276 304 367 9.81e-11 62.8
MS.gene92359.t1 AT1G68920 50.000 64 31 1 214 276 304 367 9.81e-11 62.8

Find 62 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACATTCCTCTTGTTCTT+TGG 0.181067 5.1:-285014 None:intergenic
CAGCTTTGACTATATTTCTA+TGG 0.187224 5.1:+284671 MS.gene92359:CDS
TGACATTCCTCTTGTTCTTT+GGG 0.254260 5.1:-285013 None:intergenic
TGTTGTACATGGAGTGAATT+TGG 0.258460 5.1:-284712 None:intergenic
ATTTAGCCGTAGAATCATTC+TGG 0.309924 5.1:-285184 None:intergenic
ATATAGTCAAAGCTGGTTAC+TGG 0.320414 5.1:-284663 None:intergenic
AGTTTCAGTACCCAACAAAT+TGG 0.360695 5.1:-284556 None:intergenic
AGTGAATTTGGAATGATCTA+TGG 0.365652 5.1:-284700 None:intergenic
ACCACAAGCGCAGAAAATTC+AGG 0.383330 5.1:+284807 MS.gene92359:CDS
TATCAAAATTCCAATTTGTT+GGG 0.383401 5.1:+284546 MS.gene92359:CDS
GAAAGTTACCTTAGTTCCAT+TGG 0.390559 5.1:-285675 None:intergenic
AAAGTTACCTTAGTTCCATT+GGG 0.395654 5.1:-285674 None:intergenic
CTATCAAAATTCCAATTTGT+TGG 0.398328 5.1:+284545 MS.gene92359:CDS
CTGAGCTTATTTCTCAAGAA+TGG 0.409782 5.1:+284436 MS.gene92359:CDS
GACTTTATGAACCAGTTACT+TGG 0.418424 5.1:+284495 MS.gene92359:CDS
ACGTAGCTCATGAGAAGAAT+TGG 0.419048 5.1:-285138 None:intergenic
TTCATTCCATTGTAAGCAAT+GGG 0.427456 5.1:-285984 None:intergenic
ATGAGGAGTTACCAAGTAAC+TGG 0.430149 5.1:-284506 None:intergenic
TGGGTACTGAAACTGCATTT+TGG 0.433806 5.1:+284565 MS.gene92359:CDS
GAATACAAGTCCTGGTTCTC+AGG 0.435078 5.1:+285079 MS.gene92359:CDS
TTGTTAGCTTGTGTTGTACA+TGG 0.436867 5.1:-284723 None:intergenic
GATTCTACGGCTAAATTAAG+TGG 0.448568 5.1:+285191 MS.gene92359:CDS
CCTTAGTTCCATTGGGGACA+AGG 0.451533 5.1:-285667 None:intergenic
CCTTGTCCCCAATGGAACTA+AGG 0.456805 5.1:+285667 MS.gene92359:CDS
GTTCATTCCATTGTAAGCAA+TGG 0.476694 5.1:-285985 None:intergenic
AGCTTTGACTATATTTCTAT+GGG 0.482058 5.1:+284672 MS.gene92359:CDS
ATGAACATTGGACTTGAACT+TGG 0.504815 5.1:+286002 MS.gene92359:CDS
ACAAGAGGAATGTCAAATGT+AGG 0.531678 5.1:+285021 MS.gene92359:CDS
CATAGAAATATAGTCAAAGC+TGG 0.533612 5.1:-284670 None:intergenic
GCTTACAATGGAATGAACAT+TGG 0.534664 5.1:+285990 MS.gene92359:CDS
AGGCTCTGTGGATCAGTAGC+AGG 0.543740 5.1:-285236 None:intergenic
TTCTCATGAGCTACGTGGAA+GGG 0.546048 5.1:+285145 MS.gene92359:CDS
TCTTGAGAAATAAGCTCAGT+AGG 0.547757 5.1:-284432 None:intergenic
GAAGAGAAAGAATAAATGAA+AGG 0.551355 5.1:+285627 MS.gene92359:CDS
TACTCTCCCATTGCTTACAA+TGG 0.559351 5.1:+285978 MS.gene92359:CDS
TTTGAGCCAGAATGATTCTA+CGG 0.561478 5.1:+285178 MS.gene92359:CDS
CTACAAAACCTTGTCCCCAA+TGG 0.563548 5.1:+285659 MS.gene92359:CDS
GCAACTAGTATCAGAAGTTG+AGG 0.564399 5.1:-284628 None:intergenic
ACTAGTTTCTGAACCAGAAG+AGG 0.570837 5.1:+284779 MS.gene92359:CDS
TATAGGTCCCAAAGAACAAG+AGG 0.573850 5.1:+285006 MS.gene92359:intron
ACTGAAGAGAATACAAGTCC+TGG 0.575082 5.1:+285071 MS.gene92359:CDS
AACGTCTGATCCTGAGAACC+AGG 0.576280 5.1:-285089 None:intergenic
CTTCTCATGAGCTACGTGGA+AGG 0.576985 5.1:+285144 MS.gene92359:CDS
AAGATCTAGGAGCTCAAATG+AGG 0.582801 5.1:+284833 MS.gene92359:CDS
TCTCAGGATCAGACGTTAAG+CGG 0.590949 5.1:+285095 MS.gene92359:CDS
GGATATCAGCACCATGCTTG+AGG 0.592275 5.1:+285792 MS.gene92359:CDS
GAAATGTTGTGATGAGAATG+AGG 0.597945 5.1:-284523 None:intergenic
CTAGTATCAGAAGTTGAGGT+AGG 0.600931 5.1:-284624 None:intergenic
AACTTGTGCAGTAACAATTG+TGG 0.601327 5.1:-284601 None:intergenic
GTTAAGCGGTTATTGTTCAG+AGG 0.601995 5.1:+285109 MS.gene92359:CDS
TTGGACAGCTTCCTCAAGCA+TGG 0.610000 5.1:-285803 None:intergenic
TACCCCAACAAAAGAAACAA+AGG 0.623892 5.1:+286028 MS.gene92359:CDS
AAAGACTTACTCTTGCATAG+AGG 0.624768 5.1:-285256 None:intergenic
AAGTTACCTTAGTTCCATTG+GGG 0.630156 5.1:-285673 None:intergenic
TTCTAAGCTCTGATGACATG+TGG 0.645185 5.1:+285952 MS.gene92359:CDS
GGAAAATCAAGATCTAGTAG+AGG 0.649455 5.1:+285212 MS.gene92359:CDS
CAGTGGACTCTACACTGCTG+AGG 0.657902 5.1:+284467 MS.gene92359:CDS
ACTCTTGCATAGAGGCTCTG+TGG 0.664485 5.1:-285248 None:intergenic
CAAGAATGGACTTCTCTCAG+TGG 0.692150 5.1:+284450 MS.gene92359:CDS
AATTCTTCTCATGAGCTACG+TGG 0.695539 5.1:+285140 MS.gene92359:CDS
GTAACAATTGTGGAATCATG+TGG 0.707912 5.1:-284591 None:intergenic
ACAATTGTGGAATCATGTGG+TGG 0.728994 5.1:-284588 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATTTAGAAGATTAATTCA+TGG - chr5.1:284874-284893 None:intergenic 20.0%
!! ACTATATATTGAACAACAAT+TGG + chr5.1:285721-285740 MS.gene92359:intron 20.0%
!! AGAAATAAAAAACAGTAGTA+AGG - chr5.1:284900-284919 None:intergenic 20.0%
!! CATTTAGAAGATTAATTCAT+GGG - chr5.1:284873-284892 None:intergenic 20.0%
!! GAAATAAAAAACAGTAGTAA+GGG - chr5.1:284899-284918 None:intergenic 20.0%
!! TCGATATACAAACATTAAAT+AGG - chr5.1:285331-285350 None:intergenic 20.0%
!!! GTTTTTACAACTTCAAATTA+AGG + chr5.1:285834-285853 MS.gene92359:CDS 20.0%
!!! TATCAAAATTCCAATTTGTT+GGG + chr5.1:284546-284565 MS.gene92359:CDS 20.0%
!!! TCTTTTCTATACAATACAAA+AGG - chr5.1:285609-285628 None:intergenic 20.0%
!!! TTTGAAATTAGAAAACTAGA+AGG - chr5.1:285365-285384 None:intergenic 20.0%
! AAAATTCAGGAAAAAGATCT+AGG + chr5.1:284820-284839 MS.gene92359:CDS 25.0%
! ACCTATAAAATCAAATGACA+CGG - chr5.1:284993-285012 None:intergenic 25.0%
! ATACAATACAAAAGGAAACA+TGG - chr5.1:285601-285620 None:intergenic 25.0%
! CTATCAAAATTCCAATTTGT+TGG + chr5.1:284545-284564 MS.gene92359:CDS 25.0%
! GAAGAGAAAGAATAAATGAA+AGG + chr5.1:285627-285646 MS.gene92359:CDS 25.0%
! GTGCTTATTCATAATGTATT+TGG + chr5.1:285527-285546 MS.gene92359:intron 25.0%
! GTTCCATTCAAATTCATATA+GGG - chr5.1:285929-285948 None:intergenic 25.0%
! GTTGTAAAAACTTCACATAT+TGG - chr5.1:285825-285844 None:intergenic 25.0%
! TGTTCAATATATAGTCTGAA+TGG - chr5.1:285716-285735 None:intergenic 25.0%
! TGTTCCATTCAAATTCATAT+AGG - chr5.1:285930-285949 None:intergenic 25.0%
!! AGCTTTGACTATATTTCTAT+GGG + chr5.1:284672-284691 MS.gene92359:CDS 25.0%
!! TGCATATCTTTTATGACTTA+CGG - chr5.1:285421-285440 None:intergenic 25.0%
!!! ATATCCTTTGTTTCTTTTGT+TGG - chr5.1:286035-286054 None:intergenic 25.0%
!!! ATGTTAATTGTTAAGTTGGT+AGG + chr5.1:285495-285514 MS.gene92359:intron 25.0%
!!! TATCCTTTGTTTCTTTTGTT+GGG - chr5.1:286034-286053 None:intergenic 25.0%
AAAACGAAATGCATATCACT+TGG - chr5.1:284928-284947 None:intergenic 30.0%
AAAATCCAAACCATAAACTG+TGG - chr5.1:284954-284973 None:intergenic 30.0%
AAAGTTACCTTAGTTCCATT+GGG - chr5.1:285677-285696 None:intergenic 30.0%
AATATATAGTCTGAATGGCA+TGG - chr5.1:285711-285730 None:intergenic 30.0%
CAACTTCAAATTAAGGTTTG+AGG + chr5.1:285841-285860 MS.gene92359:intron 30.0%
CATAGAAATATAGTCAAAGC+TGG - chr5.1:284673-284692 None:intergenic 30.0%
GATCCCTATATGAATTTGAA+TGG + chr5.1:285923-285942 MS.gene92359:intron 30.0%
GTGGATGTTAATTGTTAAGT+TGG + chr5.1:285491-285510 MS.gene92359:intron 30.0%
TTCAATCAAGCTCGATTAAA+AGG + chr5.1:285461-285480 MS.gene92359:intron 30.0%
TTCATTCCATTGTAAGCAAT+GGG - chr5.1:285987-286006 None:intergenic 30.0%
! AGTGAATTTGGAATGATCTA+TGG - chr5.1:284703-284722 None:intergenic 30.0%
! CAGCTTTGACTATATTTCTA+TGG + chr5.1:284671-284690 MS.gene92359:CDS 30.0%
! TTTTAAATGCAGAGAGAGAA+AGG - chr5.1:285872-285891 None:intergenic 30.0%
!! ATCCTTTGTTTCTTTTGTTG+GGG - chr5.1:286033-286052 None:intergenic 30.0%
!! TCGATTAAAAGGTGTTTATG+TGG + chr5.1:285472-285491 MS.gene92359:intron 30.0%
!!! TCCGTGTCATTTGATTTTAT+AGG + chr5.1:284989-285008 MS.gene92359:intron 30.0%
AAACATGGTAAGACCTTACA+TGG - chr5.1:285586-285605 None:intergenic 35.0%
AAAGACTTACTCTTGCATAG+AGG - chr5.1:285259-285278 None:intergenic 35.0%
AACTTGTGCAGTAACAATTG+TGG - chr5.1:284604-284623 None:intergenic 35.0%
AAGTTACCTTAGTTCCATTG+GGG - chr5.1:285676-285695 None:intergenic 35.0%
ACAAGAGGAATGTCAAATGT+AGG + chr5.1:285021-285040 MS.gene92359:CDS 35.0%
ACAAGCTAACAAAAACTTGC+AGG + chr5.1:284734-284753 MS.gene92359:CDS 35.0%
AGCAACACTTAGTTTCTCAT+AGG + chr5.1:285287-285306 MS.gene92359:intron 35.0%
AGTTTCAGTACCCAACAAAT+TGG - chr5.1:284559-284578 None:intergenic 35.0%
ATAGGACCACAAGATGTATT+TGG - chr5.1:285313-285332 None:intergenic 35.0%
ATATAGTCAAAGCTGGTTAC+TGG - chr5.1:284666-284685 None:intergenic 35.0%
ATGAACATTGGACTTGAACT+TGG + chr5.1:286002-286021 MS.gene92359:CDS 35.0%
ATTTAGCCGTAGAATCATTC+TGG - chr5.1:285187-285206 None:intergenic 35.0%
CAAATGAGGTAACAAACAGA+AGG + chr5.1:284847-284866 MS.gene92359:intron 35.0%
GAAAGTTACCTTAGTTCCAT+TGG - chr5.1:285678-285697 None:intergenic 35.0%
GAAATGTTGTGATGAGAATG+AGG - chr5.1:284526-284545 None:intergenic 35.0%
GACTTTATGAACCAGTTACT+TGG + chr5.1:284495-284514 MS.gene92359:CDS 35.0%
GATTCTACGGCTAAATTAAG+TGG + chr5.1:285191-285210 MS.gene92359:CDS 35.0%
GCTTACAATGGAATGAACAT+TGG + chr5.1:285990-286009 MS.gene92359:CDS 35.0%
GGAAAATCAAGATCTAGTAG+AGG + chr5.1:285212-285231 MS.gene92359:CDS 35.0%
GTAACAATTGTGGAATCATG+TGG - chr5.1:284594-284613 None:intergenic 35.0%
GTTCATTCCATTGTAAGCAA+TGG - chr5.1:285988-286007 None:intergenic 35.0%
TAATCGAGCTTGATTGAAAG+CGG - chr5.1:285459-285478 None:intergenic 35.0%
TACCCCAACAAAAGAAACAA+AGG + chr5.1:286028-286047 MS.gene92359:CDS 35.0%
TCTTGAGAAATAAGCTCAGT+AGG - chr5.1:284435-284454 None:intergenic 35.0%
TGACATTCCTCTTGTTCTTT+GGG - chr5.1:285016-285035 None:intergenic 35.0%
TGTTGTACATGGAGTGAATT+TGG - chr5.1:284715-284734 None:intergenic 35.0%
TTGACATTCCTCTTGTTCTT+TGG - chr5.1:285017-285036 None:intergenic 35.0%
TTTGAGCCAGAATGATTCTA+CGG + chr5.1:285178-285197 MS.gene92359:CDS 35.0%
! ACTGCTGCATTCTCTTTTAT+TGG - chr5.1:286071-286090 None:intergenic 35.0%
! ATGCAAGAGTAAGTCTTTTG+TGG + chr5.1:285261-285280 MS.gene92359:intron 35.0%
! CTGAGCTTATTTCTCAAGAA+TGG + chr5.1:284436-284455 MS.gene92359:CDS 35.0%
! GTACTTCACGTTCTGATTTT+CGG - chr5.1:284761-284780 None:intergenic 35.0%
! TACTTCACGTTCTGATTTTC+GGG - chr5.1:284760-284779 None:intergenic 35.0%
!! TTGTTAGCTTGTGTTGTACA+TGG - chr5.1:284726-284745 None:intergenic 35.0%
!! TTTTTGTTGATTAACCCTGC+AGG + chr5.1:285768-285787 MS.gene92359:intron 35.0%
AAGATCTAGGAGCTCAAATG+AGG + chr5.1:284833-284852 MS.gene92359:CDS 40.0%
AATCGAGCTTGATTGAAAGC+GGG - chr5.1:285458-285477 None:intergenic 40.0%
AATTCTTCTCATGAGCTACG+TGG + chr5.1:285140-285159 MS.gene92359:CDS 40.0%
ACAATTGTGGAATCATGTGG+TGG - chr5.1:284591-284610 None:intergenic 40.0%
ACGTAGCTCATGAGAAGAAT+TGG - chr5.1:285141-285160 None:intergenic 40.0%
ACTGAAGAGAATACAAGTCC+TGG + chr5.1:285071-285090 MS.gene92359:CDS 40.0%
ATCCAAACCATAAACTGTGG+TGG - chr5.1:284951-284970 None:intergenic 40.0%
ATGAGGAGTTACCAAGTAAC+TGG - chr5.1:284509-284528 None:intergenic 40.0%
ATTCTGTTGCTGTCCATGTA+AGG + chr5.1:285570-285589 MS.gene92359:intron 40.0%
CATAGGCCAAATACATCTTG+TGG + chr5.1:285304-285323 MS.gene92359:intron 40.0%
CTAGTATCAGAAGTTGAGGT+AGG - chr5.1:284627-284646 None:intergenic 40.0%
GCAACTAGTATCAGAAGTTG+AGG - chr5.1:284631-284650 None:intergenic 40.0%
GTTAAGCGGTTATTGTTCAG+AGG + chr5.1:285109-285128 MS.gene92359:CDS 40.0%
TACTCTCCCATTGCTTACAA+TGG + chr5.1:285978-285997 MS.gene92359:CDS 40.0%
TATAGGTCCCAAAGAACAAG+AGG + chr5.1:285006-285025 MS.gene92359:intron 40.0%
TTCCACCACAGTTTATGGTT+TGG + chr5.1:284946-284965 MS.gene92359:intron 40.0%
! ACTAGTTTCTGAACCAGAAG+AGG + chr5.1:284779-284798 MS.gene92359:CDS 40.0%
! TCGTTTTCCACCACAGTTTA+TGG + chr5.1:284941-284960 MS.gene92359:intron 40.0%
! TTCTAAGCTCTGATGACATG+TGG + chr5.1:285952-285971 MS.gene92359:CDS 40.0%
!!! CTTGTGGTTTTATCCTCTTC+TGG - chr5.1:284795-284814 None:intergenic 40.0%
!!! TGGGTACTGAAACTGCATTT+TGG + chr5.1:284565-284584 MS.gene92359:CDS 40.0%
ACCACAAGCGCAGAAAATTC+AGG + chr5.1:284807-284826 MS.gene92359:CDS 45.0%
CTACAAAACCTTGTCCCCAA+TGG + chr5.1:285659-285678 MS.gene92359:CDS 45.0%
TCTCAGGATCAGACGTTAAG+CGG + chr5.1:285095-285114 MS.gene92359:CDS 45.0%
TTCTCATGAGCTACGTGGAA+GGG + chr5.1:285145-285164 MS.gene92359:CDS 45.0%
! CAAGAATGGACTTCTCTCAG+TGG + chr5.1:284450-284469 MS.gene92359:CDS 45.0%
! GAATACAAGTCCTGGTTCTC+AGG + chr5.1:285079-285098 MS.gene92359:CDS 45.0%
! TCCTGAATTTTCTGCGCTTG+TGG - chr5.1:284811-284830 None:intergenic 45.0%
! TTGTTGATTAACCCTGCAGG+TGG + chr5.1:285771-285790 MS.gene92359:intron 45.0%
!! CATTGGGGACAAGGTTTTGT+AGG - chr5.1:285661-285680 None:intergenic 45.0%
AACGTCTGATCCTGAGAACC+AGG - chr5.1:285092-285111 None:intergenic 50.0%
ACTCTTGCATAGAGGCTCTG+TGG - chr5.1:285251-285270 None:intergenic 50.0%
CCTTAGTTCCATTGGGGACA+AGG - chr5.1:285670-285689 None:intergenic 50.0%
CCTTGTCCCCAATGGAACTA+AGG + chr5.1:285667-285686 MS.gene92359:CDS 50.0%
CTTCTCATGAGCTACGTGGA+AGG + chr5.1:285144-285163 MS.gene92359:CDS 50.0%
GGATATCAGCACCATGCTTG+AGG + chr5.1:285792-285811 MS.gene92359:CDS 50.0%
TTGGACAGCTTCCTCAAGCA+TGG - chr5.1:285806-285825 None:intergenic 50.0%
AGGCTCTGTGGATCAGTAGC+AGG - chr5.1:285239-285258 None:intergenic 55.0%
! CAGTGGACTCTACACTGCTG+AGG + chr5.1:284467-284486 MS.gene92359:CDS 55.0%
!! GGTGCTGATATCCACCTGCA+GGG - chr5.1:285785-285804 None:intergenic 55.0%
!! TGGTGCTGATATCCACCTGC+AGG - chr5.1:285786-285805 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 284426 286094 284426 ID=MS.gene92359
chr5.1 mRNA 284426 286094 284426 ID=MS.gene92359.t1;Parent=MS.gene92359
chr5.1 exon 284426 284854 284426 ID=MS.gene92359.t1.exon1;Parent=MS.gene92359.t1
chr5.1 CDS 284426 284854 284426 ID=cds.MS.gene92359.t1;Parent=MS.gene92359.t1
chr5.1 exon 285011 285268 285011 ID=MS.gene92359.t1.exon2;Parent=MS.gene92359.t1
chr5.1 CDS 285011 285268 285011 ID=cds.MS.gene92359.t1;Parent=MS.gene92359.t1
chr5.1 exon 285623 285688 285623 ID=MS.gene92359.t1.exon3;Parent=MS.gene92359.t1
chr5.1 CDS 285623 285688 285623 ID=cds.MS.gene92359.t1;Parent=MS.gene92359.t1
chr5.1 exon 285790 285855 285790 ID=MS.gene92359.t1.exon4;Parent=MS.gene92359.t1
chr5.1 CDS 285790 285855 285790 ID=cds.MS.gene92359.t1;Parent=MS.gene92359.t1
chr5.1 exon 285951 286094 285951 ID=MS.gene92359.t1.exon5;Parent=MS.gene92359.t1
chr5.1 CDS 285951 286094 285951 ID=cds.MS.gene92359.t1;Parent=MS.gene92359.t1
Gene Sequence

>MS.gene92359

ATGGAACCTACTGAGCTTATTTCTCAAGAATGGACTTCTCTCAGTGGACTCTACACTGCTGAGGAATCTGACTTTATGAACCAGTTACTTGGTAACTCCTCATTCTCATCACAACATTTCTATCAAAATTCCAATTTGTTGGGTACTGAAACTGCATTTTGGCCACCACATGATTCCACAATTGTTACTGCACAAGTTCCTACCTCAACTTCTGATACTAGTTGCTTCAACAACAATCCAGTAACCAGCTTTGACTATATTTCTATGGGTCTTTCCATAGATCATTCCAAATTCACTCCATGTACAACACAAGCTAACAAAAACTTGCAGGCCCGAAAATCAGAACGTGAAGTACTAGTTTCTGAACCAGAAGAGGATAAAACCACAAGCGCAGAAAATTCAGGAAAAAGATCTAGGAGCTCAAATGAGGTAACAAACAGAAGGCCCATGAATTAATCTTCTAAATGTTTCCCTTACTACTGTTTTTTATTTCTTCTTTCCAAGTGATATGCATTTCGTTTTCCACCACAGTTTATGGTTTGGATTTTGATTTTCTGAAATTCTCCGTGTCATTTGATTTTATAGGTCCCAAAGAACAAGAGGAATGTCAAATGTAGGAAGATTATAAGATGTGCTTCTATAAGCACTGAAGAGAATACAAGTCCTGGTTCTCAGGATCAGACGTTAAGCGGTTATTGTTCAGAGGATGAGTCCAATTCTTCTCATGAGCTACGTGGAAGGGAATCTTCAAGTTTGAGCCAGAATGATTCTACGGCTAAATTAAGTGGAAAATCAAGATCTAGTAGAGGTCCTGCTACTGATCCACAGAGCCTCTATGCAAGAGTAAGTCTTTTGTGGAATAGCAACACTTAGTTTCTCATAGGCCAAATACATCTTGTGGTCCTATTTAATGTTTGTATATCGAATCGAACTAATCCTTCTAGTTTTCTAATTTCAAATTGATCATTTATGTTAGTTAAAATGAACTTATTCCGTAAGTCATAAAAGATATGCATTATTAGTCTATTACCCGCTTTCAATCAAGCTCGATTAAAAGGTGTTTATGTGGATGTTAATTGTTAAGTTGGTAGGTTCTAAAAAGTGCTTATTCATAATGTATTTGGTCTTTCTAATAATATATGATATTCTGTTGCTGTCCATGTAAGGTCTTACCATGTTTCCTTTTGTATTGTATAGAAAAGAAGAGAAAGAATAAATGAAAGGTTGAAAATCCTACAAAACCTTGTCCCCAATGGAACTAAGGTAACTTTCTTTCTCAATGCCATGCCATTCAGACTATATATTGAACAACAATTGGTTCATCTGCTAAAAATTCTTTTATTTTTTGTTGATTAACCCTGCAGGTGGATATCAGCACCATGCTTGAGGAAGCTGTCCAATATGTGAAGTTTTTACAACTTCAAATTAAGGTTTGAGGATTCTCCTTTCTCTCTCTGCATTTAAAATTTGAGCTCAATGCAAAGACAATTACGTACTGATCCCTATATGAATTTGAATGGAACAGCTTCTAAGCTCTGATGACATGTGGATGTACTCTCCCATTGCTTACAATGGAATGAACATTGGACTTGAACTTGGTATTACCCCAACAAAAGAAACAAAGGATATGCATAACAACATACCAATAAAAGAGAATGCAGCAGTTTAA
Protein sequence

>MS.gene92359.t1

MEPTELISQEWTSLSGLYTAEESDFMNQLLGNSSFSSQHFYQNSNLLGTETAFWPPHDSTIVTAQVPTSTSDTSCFNNNPVTSFDYISMGLSIDHSKFTPCTTQANKNLQARKSEREVLVSEPEEDKTTSAENSGKRSRSSNEVPKNKRNVKCRKIIRCASISTEENTSPGSQDQTLSGYCSEDESNSSHELRGRESSSLSQNDSTAKLSGKSRSSRGPATDPQSLYARKRRERINERLKILQNLVPNGTKVDISTMLEEAVQYVKFLQLQIKLLSSDDMWMYSPIAYNGMNIGLELGITPTKETKDMHNNIPIKENAAV