Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92474.t1 | KEH26852.1 | 50.6 | 81 | 40 | 0 | 67 | 147 | 64 | 144 | 3.10E-13 | 85.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92474.t1 | A0A072UB72 | 50.6 | 81 | 40 | 0 | 67 | 147 | 64 | 144 | 2.2e-13 | 85.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene92474.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92474.t1 | MTR_5g071410 | 42.157 | 102 | 59 | 0 | 46 | 147 | 167 | 268 | 5.18e-21 | 86.7 |
| MS.gene92474.t1 | MTR_7g117585 | 39.394 | 99 | 60 | 0 | 49 | 147 | 47 | 145 | 1.18e-20 | 82.8 |
| MS.gene92474.t1 | MTR_6g477950 | 50.617 | 81 | 40 | 0 | 67 | 147 | 64 | 144 | 2.28e-20 | 85.5 |
| MS.gene92474.t1 | MTR_2g062330 | 33.594 | 128 | 70 | 3 | 20 | 147 | 26 | 138 | 8.83e-19 | 77.8 |
| MS.gene92474.t1 | MTR_2g012700 | 35.652 | 115 | 70 | 2 | 35 | 147 | 7 | 119 | 7.32e-17 | 72.4 |
| MS.gene92474.t1 | MTR_8g069780 | 37.500 | 80 | 50 | 0 | 68 | 147 | 76 | 155 | 1.08e-14 | 67.4 |
| MS.gene92474.t1 | MTR_3g117130 | 40.580 | 69 | 41 | 0 | 66 | 134 | 197 | 265 | 4.84e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGCAGGAGGACGACAAAT+TGG | 0.369744 | 7.3:-76722568 | MS.gene92474:CDS |
| CTAATTGAGGTAAGACTTCT+CGG | 0.383272 | 7.3:+76722718 | None:intergenic |
| CATTCGTGTTGTCAGCAATC+TGG | 0.385034 | 7.3:+76723047 | None:intergenic |
| AACAAAGCTAAGAAATCTTT+AGG | 0.387019 | 7.3:-76722624 | MS.gene92474:CDS |
| TCATGCTAATGACCTTGATT+AGG | 0.396314 | 7.3:-76722499 | None:intergenic |
| TGCGCTTGGTAGTAATGATT+TGG | 0.410935 | 7.3:+76722646 | None:intergenic |
| CGAGAAGTCTTACCTCAATT+AGG | 0.419615 | 7.3:-76722717 | MS.gene92474:CDS |
| GTTATCTTGTTGCCTAATTG+AGG | 0.443207 | 7.3:+76722705 | None:intergenic |
| TGTGAATCTTTGCCTAATCA+AGG | 0.458326 | 7.3:+76722487 | None:intergenic |
| GCTAAGAAATCTTTAGGAGC+AGG | 0.472784 | 7.3:-76722618 | MS.gene92474:CDS |
| GCCATCTGATGTATCAAGTA+AGG | 0.478299 | 7.3:+76722683 | None:intergenic |
| TTTCTTAGCTTTGTTGCGCT+TGG | 0.496702 | 7.3:+76722632 | None:intergenic |
| ATTAGAAGGATGGTGTGGCT+AGG | 0.518105 | 7.3:-76723076 | None:intergenic |
| TGTAAATGAGAAAGAGCTGC+AGG | 0.520669 | 7.3:-76722583 | MS.gene92474:CDS |
| CGAAAGCGAAGAAGAGGAGG+AGG | 0.529258 | 7.3:-76722960 | MS.gene92474:CDS |
| GTAAGACTTCTCGGGATACA+CGG | 0.550609 | 7.3:+76722727 | None:intergenic |
| CACTAATAAACAGCAAGTAA+TGG | 0.558500 | 7.3:-76722888 | MS.gene92474:intron |
| AGACGAAAGCGAAGAAGAGG+AGG | 0.558730 | 7.3:-76722963 | MS.gene92474:CDS |
| GATCAGCTCTGATGAGGAGA+TGG | 0.558774 | 7.3:-76723005 | MS.gene92474:CDS |
| CTAACACTGTAGGAGGATCA+AGG | 0.570132 | 7.3:+76722535 | None:intergenic |
| ACGTCAACTGCTAACACTGT+AGG | 0.578213 | 7.3:+76722525 | None:intergenic |
| TCAACTGCTAACACTGTAGG+AGG | 0.585589 | 7.3:+76722528 | None:intergenic |
| AGAAATCTTTAGGAGCAGGT+TGG | 0.589032 | 7.3:-76722614 | MS.gene92474:CDS |
| TAATTGAGGTAAGACTTCTC+GGG | 0.590285 | 7.3:+76722719 | None:intergenic |
| ACCTTACTTGATACATCAGA+TGG | 0.593255 | 7.3:-76722684 | MS.gene92474:CDS |
| AGAAGACGAAAGCGAAGAAG+AGG | 0.595279 | 7.3:-76722966 | MS.gene92474:CDS |
| TATAGAGATCAGCTCTGATG+AGG | 0.599472 | 7.3:-76723011 | MS.gene92474:CDS |
| CTTTGATTAGAAGGATGGTG+TGG | 0.650508 | 7.3:-76723081 | None:intergenic |
| AAATGAGAAAGAGCTGCAGG+AGG | 0.711877 | 7.3:-76722580 | MS.gene92474:CDS |
| ACTTCTCGGGATACACGGCG+TGG | 0.721677 | 7.3:+76722732 | None:intergenic |
| CAACACGAATGTAATCAACG+AGG | 0.787752 | 7.3:-76723035 | MS.gene92474:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAATGATTATATTTCTT+TGG | - | chr7.3:76722780-76722799 | MS.gene92474:intron | 10.0% |
| ! | AACAAAGCTAAGAAATCTTT+AGG | - | chr7.3:76722943-76722962 | MS.gene92474:CDS | 25.0% |
| CACTAATAAACAGCAAGTAA+TGG | - | chr7.3:76722679-76722698 | MS.gene92474:CDS | 30.0% | |
| !!! | CTGTCACTCTTTTTTCAAAA+TGG | + | chr7.3:76722646-76722665 | None:intergenic | 30.0% |
| ACCTTACTTGATACATCAGA+TGG | - | chr7.3:76722883-76722902 | MS.gene92474:intron | 35.0% | |
| CTAATTGAGGTAAGACTTCT+CGG | + | chr7.3:76722852-76722871 | None:intergenic | 35.0% | |
| GTTATCTTGTTGCCTAATTG+AGG | + | chr7.3:76722865-76722884 | None:intergenic | 35.0% | |
| TAATTGAGGTAAGACTTCTC+GGG | + | chr7.3:76722851-76722870 | None:intergenic | 35.0% | |
| AAAAAGAGTGACAGCTGCTA+TGG | - | chr7.3:76722652-76722671 | MS.gene92474:CDS | 40.0% | |
| CAACACGAATGTAATCAACG+AGG | - | chr7.3:76722532-76722551 | MS.gene92474:CDS | 40.0% | |
| CGAGAAGTCTTACCTCAATT+AGG | - | chr7.3:76722850-76722869 | MS.gene92474:intron | 40.0% | |
| GCCATCTGATGTATCAAGTA+AGG | + | chr7.3:76722887-76722906 | None:intergenic | 40.0% | |
| TGTAAATGAGAAAGAGCTGC+AGG | - | chr7.3:76722984-76723003 | MS.gene92474:CDS | 40.0% | |
| ! | AGAAATCTTTAGGAGCAGGT+TGG | - | chr7.3:76722953-76722972 | MS.gene92474:CDS | 40.0% |
| ! | GCTAAGAAATCTTTAGGAGC+AGG | - | chr7.3:76722949-76722968 | MS.gene92474:CDS | 40.0% |
| ! | TATAGAGATCAGCTCTGATG+AGG | - | chr7.3:76722556-76722575 | MS.gene92474:CDS | 40.0% |
| ! | TGCGCTTGGTAGTAATGATT+TGG | + | chr7.3:76722924-76722943 | None:intergenic | 40.0% |
| !! | TTTCTTAGCTTTGTTGCGCT+TGG | + | chr7.3:76722938-76722957 | None:intergenic | 40.0% |
| AAATGAGAAAGAGCTGCAGG+AGG | - | chr7.3:76722987-76723006 | MS.gene92474:CDS | 45.0% | |
| ACGTCAACTGCTAACACTGT+AGG | + | chr7.3:76723045-76723064 | None:intergenic | 45.0% | |
| AGAAGACGAAAGCGAAGAAG+AGG | - | chr7.3:76722601-76722620 | MS.gene92474:CDS | 45.0% | |
| CATTCGTGTTGTCAGCAATC+TGG | + | chr7.3:76722523-76722542 | None:intergenic | 45.0% | |
| CTAACACTGTAGGAGGATCA+AGG | + | chr7.3:76723035-76723054 | None:intergenic | 45.0% | |
| GTAAGACTTCTCGGGATACA+CGG | + | chr7.3:76722843-76722862 | None:intergenic | 45.0% | |
| TCAACTGCTAACACTGTAGG+AGG | + | chr7.3:76723042-76723061 | None:intergenic | 45.0% | |
| AGACGAAAGCGAAGAAGAGG+AGG | - | chr7.3:76722604-76722623 | MS.gene92474:CDS | 50.0% | |
| ! | GATCAGCTCTGATGAGGAGA+TGG | - | chr7.3:76722562-76722581 | MS.gene92474:CDS | 50.0% |
| CGAAAGCGAAGAAGAGGAGG+AGG | - | chr7.3:76722607-76722626 | MS.gene92474:CDS | 55.0% | |
| GCTGCAGGAGGACGACAAAT+TGG | - | chr7.3:76722999-76723018 | MS.gene92474:CDS | 55.0% | |
| ACTTCTCGGGATACACGGCG+TGG | + | chr7.3:76722838-76722857 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 76722500 | 76723089 | 76722500 | ID=MS.gene92474 |
| chr7.3 | mRNA | 76722500 | 76723089 | 76722500 | ID=MS.gene92474.t1;Parent=MS.gene92474 |
| chr7.3 | exon | 76722889 | 76723089 | 76722889 | ID=MS.gene92474.t1.exon1;Parent=MS.gene92474.t1 |
| chr7.3 | CDS | 76722889 | 76723089 | 76722889 | ID=cds.MS.gene92474.t1;Parent=MS.gene92474.t1 |
| chr7.3 | exon | 76722500 | 76722760 | 76722500 | ID=MS.gene92474.t1.exon2;Parent=MS.gene92474.t1 |
| chr7.3 | CDS | 76722500 | 76722760 | 76722500 | ID=cds.MS.gene92474.t1;Parent=MS.gene92474.t1 |
| Gene Sequence |
| Protein sequence |
