AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92496

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92496.t1 MTR_6g051425 85.757 337 36 2 1 331 1 331 0.0 592
MS.gene92496.t1 MTR_3g465760 97.143 70 2 0 40 109 1 70 7.84e-42 145
MS.gene92496.t1 MTR_4g035820 29.819 332 197 11 18 333 76 387 6.45e-32 132
MS.gene92496.t1 MTR_0558s0020 31.076 251 154 6 22 255 41 289 1.72e-29 119
MS.gene92496.t1 MTR_1g070355 32.051 234 149 5 25 255 82 308 5.12e-29 122
MS.gene92496.t1 MTR_2g039370 69.231 78 20 1 49 126 98 171 1.98e-28 115
MS.gene92496.t1 MTR_6g090505 31.579 209 121 4 64 255 21 224 8.03e-25 107
MS.gene92496.t1 MTR_8g446020 31.731 208 124 5 61 255 47 249 3.71e-24 102
MS.gene92496.t1 MTR_7g446480 32.941 170 114 0 105 274 149 318 4.71e-24 103
MS.gene92496.t1 MTR_7g446520 28.185 259 160 4 25 280 96 331 1.07e-23 103
MS.gene92496.t1 MTR_7g446510 32.544 169 114 0 105 273 164 332 1.52e-22 99.8
MS.gene92496.t1 MTR_1g083640 27.948 229 146 5 22 233 56 282 2.66e-20 92.0
MS.gene92496.t1 MTR_1g013060 26.636 214 146 5 13 222 23 229 7.58e-19 86.7
MS.gene92496.t1 MTR_5g076070 25.551 227 163 4 25 250 61 282 3.90e-18 85.5
MS.gene92496.t1 MTR_0402s0040 28.161 174 107 6 29 195 38 200 1.80e-14 76.6
MS.gene92496.t1 MTR_3g450850 24.609 256 171 6 17 263 47 289 2.39e-14 75.5
MS.gene92496.t1 MTR_6g022070 32.867 143 92 2 316 458 1 139 2.69e-14 75.1
MS.gene92496.t1 MTR_2g014100 30.049 203 123 7 22 205 49 251 2.72e-13 70.9
MS.gene92496.t1 MTR_1g008290 26.316 114 83 1 105 218 32 144 5.97e-13 67.0
MS.gene92496.t1 MTR_0337s0040 26.374 182 116 4 61 229 48 224 1.11e-11 65.5
MS.gene92496.t1 MTR_2g098920 31.579 114 70 3 11 124 77 182 5.62e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92496.t1 AT4G38180 33.925 563 359 8 2 561 62 614 2.58e-105 337
MS.gene92496.t1 AT4G15090 30.699 544 357 9 25 561 59 589 2.95e-82 276
MS.gene92496.t1 AT4G15090 30.699 544 357 9 25 561 59 589 2.95e-82 276
MS.gene92496.t1 AT4G15090 30.699 544 357 9 25 561 59 589 2.95e-82 276
MS.gene92496.t1 AT4G15090 30.699 544 357 9 25 561 59 589 2.95e-82 276
MS.gene92496.t1 AT4G15090 30.699 544 357 9 25 561 59 589 2.95e-82 276
MS.gene92496.t1 AT2G27110 29.596 544 347 8 19 561 51 559 6.81e-81 273
MS.gene92496.t1 AT2G27110 29.596 544 347 8 19 561 51 559 6.81e-81 273
MS.gene92496.t1 AT3G06250 29.004 562 343 11 2 561 177 684 6.32e-74 253
MS.gene92496.t1 AT3G06250 29.004 562 343 11 2 561 177 684 6.32e-74 253
MS.gene92496.t1 AT3G06250 29.004 562 343 11 2 561 177 684 6.32e-74 253
MS.gene92496.t1 AT2G27110 34.400 375 241 4 188 561 44 414 2.05e-72 248
MS.gene92496.t1 AT1G76320 28.942 539 362 10 25 561 5 524 2.67e-71 244
MS.gene92496.t1 AT3G22170 28.957 556 345 11 20 558 73 595 3.27e-71 247
MS.gene92496.t1 AT3G22170 28.957 556 345 11 20 558 73 595 3.27e-71 247
MS.gene92496.t1 AT1G76320 28.942 539 362 10 25 561 5 524 9.83e-71 243
MS.gene92496.t1 AT1G76320 28.942 539 362 10 25 561 5 524 1.23e-70 243
MS.gene92496.t1 AT1G76320 28.942 539 362 10 25 561 5 524 1.23e-70 243
MS.gene92496.t1 AT4G19990 28.007 582 355 11 1 561 1 539 3.79e-67 234
MS.gene92496.t1 AT4G19990 28.007 582 355 11 1 561 1 539 4.35e-67 234
MS.gene92496.t1 AT4G19990 28.007 582 355 11 1 561 1 539 4.35e-67 234
MS.gene92496.t1 AT5G18960 28.393 560 345 12 5 561 202 708 5.44e-67 234
MS.gene92496.t1 AT5G18960 28.393 560 345 12 5 561 202 708 5.44e-67 234
MS.gene92496.t1 AT4G19990 28.007 582 355 11 1 561 1 539 8.37e-67 234
MS.gene92496.t1 AT4G19990 28.007 582 355 11 1 561 1 539 8.37e-67 234
MS.gene92496.t1 AT4G19990 28.028 578 352 11 5 561 19 553 1.86e-66 233
MS.gene92496.t1 AT4G19990 28.803 493 304 8 74 561 32 482 9.51e-63 221
MS.gene92496.t1 AT1G52520 27.240 558 380 7 10 561 81 618 4.51e-62 219
MS.gene92496.t1 AT1G10240 29.286 560 353 15 18 558 49 584 7.95e-60 213
MS.gene92496.t1 AT1G10240 29.286 560 353 15 18 558 49 584 7.95e-60 213
MS.gene92496.t1 AT1G80010 26.944 553 378 10 20 558 98 638 2.27e-58 209
MS.gene92496.t1 AT5G28530 26.678 566 371 11 18 562 56 598 4.69e-58 207
MS.gene92496.t1 AT5G28530 26.678 566 371 11 18 562 56 598 4.69e-58 207
MS.gene92496.t1 AT5G28530 27.018 570 368 12 18 562 56 602 1.63e-57 206
MS.gene92496.t1 AT4G38170 29.508 366 250 5 199 562 21 380 2.09e-57 203
MS.gene92496.t1 AT2G32250 25.741 540 350 8 20 558 41 530 1.14e-54 199
MS.gene92496.t1 AT2G32250 25.556 540 351 8 20 558 41 530 1.98e-54 199
MS.gene92496.t1 AT2G32250 25.556 540 351 8 20 558 41 530 1.98e-54 199
MS.gene92496.t1 AT2G32250 25.556 540 351 8 20 558 41 530 1.98e-54 199
MS.gene92496.t1 AT2G32250 25.556 540 351 8 20 558 41 530 2.25e-54 199
MS.gene92496.t1 AT2G32250 25.556 540 351 8 20 558 41 530 2.25e-54 199
MS.gene92496.t1 AT2G32250 25.607 535 347 8 25 558 5 489 2.34e-54 199
MS.gene92496.t1 AT2G32250 25.794 535 346 8 25 558 5 489 2.46e-54 199
MS.gene92496.t1 AT5G28530 23.339 557 338 13 18 562 56 535 7.59e-32 131
MS.gene92496.t1 AT5G28530 23.708 561 335 14 18 562 56 539 2.04e-31 130

Find 107 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAATGCACCTTCTCATTT+TGG 0.108203 7.3:+77300619 MS.gene92496:CDS
ACTAATGTTTGGAGTTATTT+AGG 0.178667 7.3:+77300073 MS.gene92496:CDS
AGGGTTATCATTCCTCAAAT+TGG 0.230210 7.3:+77302041 MS.gene92496:intron
TGTATAAAATTCGTCATAAA+TGG 0.250035 7.3:+77300782 MS.gene92496:CDS
CATAAACGGTGACATGTATT+TGG 0.254781 7.3:-77300580 None:intergenic
AAAAGTTTCATGGTTTGTTT+TGG 0.257014 7.3:+77300659 MS.gene92496:CDS
AACTCTGATCAAAAGTTTCA+TGG 0.276945 7.3:+77300649 MS.gene92496:CDS
CCATCTTGAAGCCATGTTCT+TGG 0.280191 7.3:-77299706 None:intergenic
CACATGTAACTCATTGATTC+AGG 0.282301 7.3:+77302114 MS.gene92496:CDS
AATGTTCCTTCTACTTCTTT+TGG 0.283406 7.3:+77301919 MS.gene92496:CDS
ACTACCACATGTTTCTTATT+AGG 0.296197 7.3:-77301174 None:intergenic
CACGTTTAACCAATGTGTTT+TGG 0.307246 7.3:+77300275 MS.gene92496:CDS
GGGTTATCATTCCTCAAATT+GGG 0.312307 7.3:+77302042 MS.gene92496:intron
GCGAATTACTAAGTTGATAA+TGG 0.317295 7.3:+77299934 MS.gene92496:CDS
GAAAAGAGTAAATTAATTAA+AGG 0.318587 7.3:+77300163 MS.gene92496:CDS
AAAGTTGATTATGATTGTTT+TGG 0.323388 7.3:+77300313 MS.gene92496:CDS
CATTGGTTAAACGTGATTCT+TGG 0.328206 7.3:-77300267 None:intergenic
AAACTTCTGTTTCTTTCAAT+TGG 0.330619 7.3:+77300458 MS.gene92496:CDS
ATTGTTCTTAAACCCTTGTT+TGG 0.337062 7.3:-77299807 None:intergenic
CATGTCACCGTTTATGCTTA+TGG 0.342415 7.3:+77300587 MS.gene92496:CDS
ACTAAGATGTTACAAGGAAT+TGG 0.345022 7.3:+77302091 MS.gene92496:CDS
CTTATTAGCATTTGAAAAGA+TGG 0.359804 7.3:+77300921 MS.gene92496:CDS
TCACCTACGATTTCTCCTTC+TGG 0.371892 7.3:-77299833 None:intergenic
GCAAGTGATTGATTAAAGAT+TGG 0.373633 7.3:-77300195 None:intergenic
TTGTCGAAAGTTTGATTCAA+TGG 0.378573 7.3:+77301230 MS.gene92496:CDS
CCCATCCATGGAAGAACTAA+TGG 0.382474 7.3:-77301860 None:intergenic
CAAGTGATTGATTAAAGATT+GGG 0.384672 7.3:-77300194 None:intergenic
GTATGAAAGTTACTAATGTT+TGG 0.386893 7.3:+77300062 MS.gene92496:CDS
AGACTTTATGAGACCTCCTT+TGG 0.388927 7.3:-77300026 None:intergenic
CAAGTAGTGCTTTCCAAGAT+AGG 0.389222 7.3:+77302232 MS.gene92496:CDS
TCCATTAGTTCTTCCATGGA+TGG 0.401531 7.3:+77301859 MS.gene92496:CDS
AGGAGGTCTCATAAAGTCTA+TGG 0.403748 7.3:+77300030 MS.gene92496:CDS
GAAGTACTTCTAAAGACATT+GGG 0.403994 7.3:+77301106 MS.gene92496:CDS
TAACATCTTAGTAAATGAAA+TGG 0.407933 7.3:-77302080 None:intergenic
AAAACAATCATAATCAACTT+TGG 0.409741 7.3:-77300311 None:intergenic
ACTTAGACTGGTTGCTGCTC+CGG 0.416105 7.3:-77300893 None:intergenic
CATTTGCAAAATGTCATGTT+TGG 0.424549 7.3:+77300415 MS.gene92496:CDS
CATAACGTCTCCGATTGCTT+TGG 0.425284 7.3:-77300557 None:intergenic
AATGAGTATCTTGCTGGAAC+TGG 0.425528 7.3:+77301081 MS.gene92496:CDS
CTATCTTGGAAAGCACTACT+TGG 0.431144 7.3:-77302231 None:intergenic
CCATTAGTTCTTCCATGGAT+GGG 0.443746 7.3:+77301860 MS.gene92496:CDS
CTTATCAAAGGGCTGATTCA+AGG 0.444079 7.3:+77299861 MS.gene92496:CDS
TGTCGAAAGTTTGATTCAAT+GGG 0.444829 7.3:+77301231 MS.gene92496:CDS
TTGTTCCATTAGTTCTTCCA+TGG 0.449933 7.3:+77301855 MS.gene92496:CDS
CGATTTAATGTTAGCAAGGA+TGG 0.456458 7.3:+77299905 MS.gene92496:CDS
CTCACTGATATTTATGATGC+GGG 0.456755 7.3:-77301004 None:intergenic
GATTCAAAAGAAGAGTTTGA+AGG 0.459181 7.3:+77300700 MS.gene92496:CDS
ATGTTATTGAGAACATTAGA+AGG 0.459905 7.3:-77301883 None:intergenic
TATTTAGGAGAAGAGGTTGG+TGG 0.461595 7.3:+77300088 MS.gene92496:CDS
GGTGCACCAAAAGAAGTAGA+AGG 0.473964 7.3:-77301925 None:intergenic
ATATATGCCATAAGCATAAA+CGG 0.475933 7.3:-77300594 None:intergenic
AGTGTGTTAAGTGCGATTGC+CGG 0.478839 7.3:+77300874 MS.gene92496:CDS
AGTATTTAAACCCCAATTTG+AGG 0.480130 7.3:-77302053 None:intergenic
TGATGCGGGGAGGCATACTT+TGG 0.480707 7.3:-77300990 None:intergenic
GATAAATTATAAACCTCTAA+AGG 0.488005 7.3:-77301946 None:intergenic
GTTGGTGGATTTGATAAGCT+TGG 0.492644 7.3:+77300103 MS.gene92496:CDS
TGATGATTTACCCCAACAAA+AGG 0.496863 7.3:-77300394 None:intergenic
AAGAAGAGTTTGAAGGCACT+TGG 0.500098 7.3:+77300707 MS.gene92496:CDS
CAAGAACATGGCTTCAAGAT+GGG 0.500638 7.3:+77299707 MS.gene92496:CDS
ACAAGCTTCAAAATCACAAA+TGG 0.503549 7.3:+77300749 MS.gene92496:CDS
CCAAGAACATGGCTTCAAGA+TGG 0.507821 7.3:+77299706 MS.gene92496:CDS
AGTTATTTAGGAGAAGAGGT+TGG 0.508257 7.3:+77300085 MS.gene92496:CDS
GGAGCCATGGCCAAAGCAAT+CGG 0.517653 7.3:+77300547 MS.gene92496:CDS
GTCTCCGATTGCTTTGGCCA+TGG 0.517925 7.3:-77300551 None:intergenic
TTGCGAATTTACAACATCAA+AGG 0.519182 7.3:+77301273 MS.gene92496:CDS
GGAAGTACTTCTAAAGACAT+TGG 0.522331 7.3:+77301105 MS.gene92496:CDS
TCATTTACTAAGATGTTACA+AGG 0.524417 7.3:+77302085 MS.gene92496:CDS
TTGGAGTTATTTAGGAGAAG+AGG 0.526196 7.3:+77300081 MS.gene92496:CDS
GAGTATCTTGCTGGAACTGG+TGG 0.526825 7.3:+77301084 MS.gene92496:CDS
GATTATTATTGTTCCAAACA+AGG 0.530481 7.3:+77299794 MS.gene92496:CDS
ATGTTAGCAAGGATGGAGAA+TGG 0.532233 7.3:+77299912 MS.gene92496:CDS
TAGGTGAAGTTGCTTATCAA+AGG 0.533461 7.3:+77299849 MS.gene92496:CDS
AAGAGTAAATTAATTAAAGG+TGG 0.536645 7.3:+77300166 MS.gene92496:CDS
AATGGATCACAACCATGCTT+TGG 0.538091 7.3:+77299952 MS.gene92496:CDS
TAATAAGAAATGACTTAGAC+TGG 0.538323 7.3:-77300905 None:intergenic
ATTATTATTGTTCCAAACAA+GGG 0.540953 7.3:+77299795 MS.gene92496:CDS
AGGTGAAGTTGCTTATCAAA+GGG 0.546039 7.3:+77299850 MS.gene92496:CDS
AACATTAGAAGGCCCATCCA+TGG 0.546919 7.3:-77301872 None:intergenic
TACAGATCAAGATGGAGCCA+TGG 0.551336 7.3:+77300534 MS.gene92496:CDS
TCAAATCATATGTATTAGTA+CGG 0.557489 7.3:-77300363 None:intergenic
GCGACAATTAGAGACTGAAA+AGG 0.558235 7.3:+77300954 MS.gene92496:CDS
ATATGTATTAGTACGGTAAG+TGG 0.559004 7.3:-77300356 None:intergenic
AAGGACAAATTGTAAAGCAA+TGG 0.564077 7.3:+77299880 MS.gene92496:CDS
ATAAAGTCTATGGTGAATGC+TGG 0.565519 7.3:+77300040 MS.gene92496:CDS
GCTCGAGCGGCACCAAAGCA+TGG 0.565748 7.3:-77299964 None:intergenic
GGTTATCATTCCTCAAATTG+GGG 0.572895 7.3:+77302043 MS.gene92496:CDS
TGGTCAAGTTGTGCATAGCA+AGG 0.581013 7.3:+77299664 MS.gene92496:CDS
TTCTAATGTTCTCAATAACA+TGG 0.585453 7.3:+77301885 MS.gene92496:CDS
ACCATCTCTCCAAAACACAT+TGG 0.590535 7.3:-77300284 None:intergenic
GGTGCGATTTAATGTTAGCA+AGG 0.593147 7.3:+77299901 MS.gene92496:CDS
ATCTTAGTAAATGAAATGGA+TGG 0.594963 7.3:-77302076 None:intergenic
TCTCACTGATATTTATGATG+CGG 0.607441 7.3:-77301005 None:intergenic
TTAAGATCACCAAAATGAGA+AGG 0.607446 7.3:-77300628 None:intergenic
CTGCACACTATAGTAGAACA+TGG 0.618082 7.3:-77300239 None:intergenic
TTGAAAAGATGGTAAACAAG+TGG 0.620155 7.3:+77300932 MS.gene92496:CDS
TTTAAGAACAATGAACCAGA+AGG 0.621796 7.3:+77299818 MS.gene92496:CDS
ATGGAGCACTGCATATAGCA+AGG 0.629512 7.3:+77300801 MS.gene92496:CDS
ACAACTGATCGTTGCAGCAG+AGG 0.638921 7.3:-77301205 None:intergenic
GAACCAGAAGGAGAAATCGT+AGG 0.643994 7.3:+77299830 MS.gene92496:CDS
AGAAAAGTATCAGAAACCAA+AGG 0.645801 7.3:+77300010 MS.gene92496:CDS
AACAAATGTCTTTGCTCGAG+CGG 0.656974 7.3:-77299977 None:intergenic
TATAACTTATACCAAGAACA+TGG 0.669118 7.3:+77299695 MS.gene92496:CDS
AAGTACTTCTAAAGACATTG+GGG 0.673250 7.3:+77301107 MS.gene92496:CDS
TATGCCTAATAAGAAACATG+TGG 0.676676 7.3:+77301170 MS.gene92496:CDS
CTGATATTTATGATGCGGGG+AGG 0.680172 7.3:-77301000 None:intergenic
AAAGTATCAGAAACCAAAGG+AGG 0.690747 7.3:+77300013 MS.gene92496:CDS
TCACTGATATTTATGATGCG+GGG 0.700675 7.3:-77301003 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTACATAATTTAATA+GGG + chr7.3:77302022-77302041 MS.gene92496:intron 10.0%
!! CTATTAAATTATGTAAAAGA+AGG - chr7.3:77302023-77302042 None:intergenic 15.0%
!! GAAAAGAGTAAATTAATTAA+AGG + chr7.3:77300163-77300182 MS.gene92496:CDS 15.0%
!!! CTTCTTTTACATAATTTAAT+AGG + chr7.3:77302021-77302040 MS.gene92496:intron 15.0%
!!! GTTTTTTTATAATCTAGTTT+TGG + chr7.3:77301461-77301480 MS.gene92496:intron 15.0%
!! AAAAAATTTCACTCAAAATG+AGG + chr7.3:77301602-77301621 MS.gene92496:intron 20.0%
!! AAAACAATCATAATCAACTT+TGG - chr7.3:77300314-77300333 None:intergenic 20.0%
!! AAGAGTAAATTAATTAAAGG+TGG + chr7.3:77300166-77300185 MS.gene92496:CDS 20.0%
!! ACTTAAAAGTTATCAAGAAA+TGG - chr7.3:77301973-77301992 None:intergenic 20.0%
!! ATTATTATTGTTCCAAACAA+GGG + chr7.3:77299795-77299814 MS.gene92496:CDS 20.0%
!! GATAAATTATAAACCTCTAA+AGG - chr7.3:77301949-77301968 None:intergenic 20.0%
!! TAACATCTTAGTAAATGAAA+TGG - chr7.3:77302083-77302102 None:intergenic 20.0%
!! TCAAATCATATGTATTAGTA+CGG - chr7.3:77300366-77300385 None:intergenic 20.0%
!! TGTATAAAATTCGTCATAAA+TGG + chr7.3:77300782-77300801 MS.gene92496:CDS 20.0%
!!! AAAGTTGATTATGATTGTTT+TGG + chr7.3:77300313-77300332 MS.gene92496:CDS 20.0%
!!! TGATCTGTAAAAAATGTTTT+TGG - chr7.3:77300523-77300542 None:intergenic 20.0%
! AGTTATGAATGATTTCATTG+TGG + chr7.3:77301990-77302009 MS.gene92496:intron 25.0%
! ATATATGCCATAAGCATAAA+CGG - chr7.3:77300597-77300616 None:intergenic 25.0%
! ATCTTAGTAAATGAAATGGA+TGG - chr7.3:77302079-77302098 None:intergenic 25.0%
! ATGTTATTGAGAACATTAGA+AGG - chr7.3:77301886-77301905 None:intergenic 25.0%
! CAAGTGATTGATTAAAGATT+GGG - chr7.3:77300197-77300216 None:intergenic 25.0%
! CTTATTAGCATTTGAAAAGA+TGG + chr7.3:77300921-77300940 MS.gene92496:CDS 25.0%
! GATTATTATTGTTCCAAACA+AGG + chr7.3:77299794-77299813 MS.gene92496:CDS 25.0%
! GTATGAAAGTTACTAATGTT+TGG + chr7.3:77300062-77300081 MS.gene92496:CDS 25.0%
! TAATAAGAAATGACTTAGAC+TGG - chr7.3:77300908-77300927 None:intergenic 25.0%
! TATAACTTATACCAAGAACA+TGG + chr7.3:77299695-77299714 MS.gene92496:CDS 25.0%
! TCATTTACTAAGATGTTACA+AGG + chr7.3:77302085-77302104 MS.gene92496:CDS 25.0%
! TTCTAATGTTCTCAATAACA+TGG + chr7.3:77301885-77301904 MS.gene92496:CDS 25.0%
!! AAACTTCTGTTTCTTTCAAT+TGG + chr7.3:77300458-77300477 MS.gene92496:CDS 25.0%
!! ACTAATGTTTGGAGTTATTT+AGG + chr7.3:77300073-77300092 MS.gene92496:CDS 25.0%
!!! AAAAGTTTCATGGTTTGTTT+TGG + chr7.3:77300659-77300678 MS.gene92496:CDS 25.0%
!!! ACATTTTTTACAGATCAAGA+TGG + chr7.3:77300526-77300545 MS.gene92496:CDS 25.0%
!!! TTGAGTGAAATTTTTTTCAC+TGG - chr7.3:77301598-77301617 None:intergenic 25.0%
!!! TTGGCTATTTAAAGTGTTTT+TGG + chr7.3:77300477-77300496 MS.gene92496:CDS 25.0%
AACTCTGATCAAAAGTTTCA+TGG + chr7.3:77300649-77300668 MS.gene92496:CDS 30.0%
AAGAAACTCAACAAATCATG+TGG + chr7.3:77301499-77301518 MS.gene92496:intron 30.0%
AAGATGGTCAAAAAAAGCAA+GGG + chr7.3:77301329-77301348 MS.gene92496:intron 30.0%
AAGGACAAATTGTAAAGCAA+TGG + chr7.3:77299880-77299899 MS.gene92496:CDS 30.0%
ACAAGCTTCAAAATCACAAA+TGG + chr7.3:77300749-77300768 MS.gene92496:CDS 30.0%
ACTAAGATGTTACAAGGAAT+TGG + chr7.3:77302091-77302110 MS.gene92496:CDS 30.0%
ACTACCACATGTTTCTTATT+AGG - chr7.3:77301177-77301196 None:intergenic 30.0%
AGTATTTAAACCCCAATTTG+AGG - chr7.3:77302056-77302075 None:intergenic 30.0%
ATATGTATTAGTACGGTAAG+TGG - chr7.3:77300359-77300378 None:intergenic 30.0%
ATTCAGGTATGTATGTATGT+AGG + chr7.3:77302130-77302149 MS.gene92496:intron 30.0%
ATTGTTCTTAAACCCTTGTT+TGG - chr7.3:77299810-77299829 None:intergenic 30.0%
CATTTGCAAAATGTCATGTT+TGG + chr7.3:77300415-77300434 MS.gene92496:CDS 30.0%
GATTCAAAAGAAGAGTTTGA+AGG + chr7.3:77300700-77300719 MS.gene92496:CDS 30.0%
GCAAGTGATTGATTAAAGAT+TGG - chr7.3:77300198-77300217 None:intergenic 30.0%
TATGCCTAATAAGAAACATG+TGG + chr7.3:77301170-77301189 MS.gene92496:CDS 30.0%
TATTCAGTTATATTGCATGC+AGG + chr7.3:77302203-77302222 MS.gene92496:intron 30.0%
TCAACAAATCATGTGGAAAT+TGG + chr7.3:77301506-77301525 MS.gene92496:intron 30.0%
TCAGAAAATGCAATGAACAA+TGG + chr7.3:77301399-77301418 MS.gene92496:intron 30.0%
TCATAATAGCATTGCGATAA+AGG - chr7.3:77301440-77301459 None:intergenic 30.0%
TCTCACTGATATTTATGATG+CGG - chr7.3:77301008-77301027 None:intergenic 30.0%
TGACCAAGAAAAATATCTCT+GGG - chr7.3:77299650-77299669 None:intergenic 30.0%
TGTCGAAAGTTTGATTCAAT+GGG + chr7.3:77301231-77301250 MS.gene92496:CDS 30.0%
TTAAGATCACCAAAATGAGA+AGG - chr7.3:77300631-77300650 None:intergenic 30.0%
TTGAAAAGATGGTAAACAAG+TGG + chr7.3:77300932-77300951 MS.gene92496:CDS 30.0%
TTGACCAAGAAAAATATCTC+TGG - chr7.3:77299651-77299670 None:intergenic 30.0%
TTGCGAATTTACAACATCAA+AGG + chr7.3:77301273-77301292 MS.gene92496:CDS 30.0%
TTGTCGAAAGTTTGATTCAA+TGG + chr7.3:77301230-77301249 MS.gene92496:CDS 30.0%
TTTAAGAACAATGAACCAGA+AGG + chr7.3:77299818-77299837 MS.gene92496:CDS 30.0%
! AAGTACTTCTAAAGACATTG+GGG + chr7.3:77301107-77301126 MS.gene92496:CDS 30.0%
! AATGTTCCTTCTACTTCTTT+TGG + chr7.3:77301919-77301938 MS.gene92496:CDS 30.0%
! ATCATGTGGAAATTGGTTTA+TGG + chr7.3:77301513-77301532 MS.gene92496:intron 30.0%
! GAAGTACTTCTAAAGACATT+GGG + chr7.3:77301106-77301125 MS.gene92496:CDS 30.0%
! TTTTATGTTCTTGCCTATCT+TGG - chr7.3:77302248-77302267 None:intergenic 30.0%
! TTTTTGAATGAGTATCTTGC+TGG + chr7.3:77301075-77301094 MS.gene92496:CDS 30.0%
!! AGAAAAGTATCAGAAACCAA+AGG + chr7.3:77300010-77300029 MS.gene92496:CDS 30.0%
!! GCGAATTACTAAGTTGATAA+TGG + chr7.3:77299934-77299953 MS.gene92496:CDS 30.0%
!!! AAGGGCATTTTGAAACAAAA+AGG + chr7.3:77301694-77301713 MS.gene92496:intron 30.0%
AATTGAGCTCATTGATGCAT+TGG - chr7.3:77301557-77301576 None:intergenic 35.0%
AGGGTTATCATTCCTCAAAT+TGG + chr7.3:77302041-77302060 MS.gene92496:intron 35.0%
AGGTGAAGTTGCTTATCAAA+GGG + chr7.3:77299850-77299869 MS.gene92496:CDS 35.0%
AGTTATTTAGGAGAAGAGGT+TGG + chr7.3:77300085-77300104 MS.gene92496:CDS 35.0%
ATAAAGTCTATGGTGAATGC+TGG + chr7.3:77300040-77300059 MS.gene92496:CDS 35.0%
CATAAACGGTGACATGTATT+TGG - chr7.3:77300583-77300602 None:intergenic 35.0%
CGATTTAATGTTAGCAAGGA+TGG + chr7.3:77299905-77299924 MS.gene92496:CDS 35.0%
CTCACTGATATTTATGATGC+GGG - chr7.3:77301007-77301026 None:intergenic 35.0%
GAAAATGCAATGAACAATGG+TGG + chr7.3:77301402-77301421 MS.gene92496:intron 35.0%
GAAGATGGTCAAAAAAAGCA+AGG + chr7.3:77301328-77301347 MS.gene92496:intron 35.0%
GGGTTATCATTCCTCAAATT+GGG + chr7.3:77302042-77302061 MS.gene92496:intron 35.0%
GGTTATCATTCCTCAAATTG+GGG + chr7.3:77302043-77302062 MS.gene92496:CDS 35.0%
TAGGTGAAGTTGCTTATCAA+AGG + chr7.3:77299849-77299868 MS.gene92496:CDS 35.0%
TCACTGATATTTATGATGCG+GGG - chr7.3:77301006-77301025 None:intergenic 35.0%
TGATGATTTACCCCAACAAA+AGG - chr7.3:77300397-77300416 None:intergenic 35.0%
TTGGAGTTATTTAGGAGAAG+AGG + chr7.3:77300081-77300100 MS.gene92496:CDS 35.0%
! AAAGTATCAGAAACCAAAGG+AGG + chr7.3:77300013-77300032 MS.gene92496:CDS 35.0%
! AAGCCCAGAGATATTTTTCT+TGG + chr7.3:77299644-77299663 MS.gene92496:CDS 35.0%
! ACCAATACTTTCTGAGAAGA+TGG + chr7.3:77301313-77301332 MS.gene92496:intron 35.0%
! ACCATCTTCTCAGAAAGTAT+TGG - chr7.3:77301317-77301336 None:intergenic 35.0%
! ATGTGGAAATTGGTTTATGG+TGG + chr7.3:77301516-77301535 MS.gene92496:intron 35.0%
! CACGTTTAACCAATGTGTTT+TGG + chr7.3:77300275-77300294 MS.gene92496:CDS 35.0%
! CATTGGTTAAACGTGATTCT+TGG - chr7.3:77300270-77300289 None:intergenic 35.0%
! TGATATGTGCTCCTTTTGTT+GGG + chr7.3:77300383-77300402 MS.gene92496:CDS 35.0%
! TTGATATGTGCTCCTTTTGT+TGG + chr7.3:77300382-77300401 MS.gene92496:CDS 35.0%
! TTGTTCCATTAGTTCTTCCA+TGG + chr7.3:77301855-77301874 MS.gene92496:CDS 35.0%
! TTTTAGTGTGAGAAAAGGGA+AGG + chr7.3:77299730-77299749 MS.gene92496:CDS 35.0%
!! ATGGGTTTTAGTGTGAGAAA+AGG + chr7.3:77299725-77299744 MS.gene92496:CDS 35.0%
!! CACATGTAACTCATTGATTC+AGG + chr7.3:77302114-77302133 MS.gene92496:CDS 35.0%
!! GGAAGTACTTCTAAAGACAT+TGG + chr7.3:77301105-77301124 MS.gene92496:CDS 35.0%
!! TGGGTTTTAGTGTGAGAAAA+GGG + chr7.3:77299726-77299745 MS.gene92496:CDS 35.0%
!!! AAAGTGTTTTTGGAGAGCAT+GGG + chr7.3:77300487-77300506 MS.gene92496:CDS 35.0%
!!! TAAAGTGTTTTTGGAGAGCA+TGG + chr7.3:77300486-77300505 MS.gene92496:CDS 35.0%
!!! TAATCTAGTTTTGGATAGCC+AGG + chr7.3:77301470-77301489 MS.gene92496:intron 35.0%
!!! TGATTGTTTTGGAGATGTTG+TGG + chr7.3:77300324-77300343 MS.gene92496:CDS 35.0%
!!! TTGTTTTGGAAATGCATGTC+AGG + chr7.3:77300673-77300692 MS.gene92496:CDS 35.0%
AACAAATGTCTTTGCTCGAG+CGG - chr7.3:77299980-77299999 None:intergenic 40.0%
AATGAGTATCTTGCTGGAAC+TGG + chr7.3:77301081-77301100 MS.gene92496:CDS 40.0%
AATGGATCACAACCATGCTT+TGG + chr7.3:77299952-77299971 MS.gene92496:CDS 40.0%
ACCATCTCTCCAAAACACAT+TGG - chr7.3:77300287-77300306 None:intergenic 40.0%
AGACTTTATGAGACCTCCTT+TGG - chr7.3:77300029-77300048 None:intergenic 40.0%
AGCATTGCGATAAAGGATCA+TGG - chr7.3:77301433-77301452 None:intergenic 40.0%
AGGAGGTCTCATAAAGTCTA+TGG + chr7.3:77300030-77300049 MS.gene92496:CDS 40.0%
AGGTCTAATACTCGCATGAA+AGG + chr7.3:77301675-77301694 MS.gene92496:intron 40.0%
ATGTTAGCAAGGATGGAGAA+TGG + chr7.3:77299912-77299931 MS.gene92496:CDS 40.0%
CAAGAACATGGCTTCAAGAT+GGG + chr7.3:77299707-77299726 MS.gene92496:CDS 40.0%
CAAGTAGTGCTTTCCAAGAT+AGG + chr7.3:77302232-77302251 MS.gene92496:CDS 40.0%
CATGTCACCGTTTATGCTTA+TGG + chr7.3:77300587-77300606 MS.gene92496:CDS 40.0%
CTGCACACTATAGTAGAACA+TGG - chr7.3:77300242-77300261 None:intergenic 40.0%
GCGACAATTAGAGACTGAAA+AGG + chr7.3:77300954-77300973 MS.gene92496:CDS 40.0%
GGTCTAATACTCGCATGAAA+GGG + chr7.3:77301676-77301695 MS.gene92496:intron 40.0%
GGTGCGATTTAATGTTAGCA+AGG + chr7.3:77299901-77299920 MS.gene92496:CDS 40.0%
GTTGAGTTTCTTTGTGTTCC+TGG - chr7.3:77301491-77301510 None:intergenic 40.0%
TATTTAGGAGAAGAGGTTGG+TGG + chr7.3:77300088-77300107 MS.gene92496:CDS 40.0%
! CAGAATGCACCTTCTCATTT+TGG + chr7.3:77300619-77300638 MS.gene92496:CDS 40.0%
! CCATTAGTTCTTCCATGGAT+GGG + chr7.3:77301860-77301879 MS.gene92496:CDS 40.0%
! CTTATCAAAGGGCTGATTCA+AGG + chr7.3:77299861-77299880 MS.gene92496:CDS 40.0%
! GATATGTGCTCCTTTTGTTG+GGG + chr7.3:77300384-77300403 MS.gene92496:CDS 40.0%
! TCCATTAGTTCTTCCATGGA+TGG + chr7.3:77301859-77301878 MS.gene92496:CDS 40.0%
!! AAGAAGAGTTTGAAGGCACT+TGG + chr7.3:77300707-77300726 MS.gene92496:CDS 40.0%
!! CTATCTTGGAAAGCACTACT+TGG - chr7.3:77302234-77302253 None:intergenic 40.0%
!! CTTCTCAGAAAGTATTGGTC+AGG - chr7.3:77301312-77301331 None:intergenic 40.0%
!! GTTGGTGGATTTGATAAGCT+TGG + chr7.3:77300103-77300122 MS.gene92496:CDS 40.0%
!! TTCTTTTGGTGCACCTTTAG+AGG + chr7.3:77301933-77301952 MS.gene92496:CDS 40.0%
!!! ACCAATGTGTTTTGGAGAGA+TGG + chr7.3:77300283-77300302 MS.gene92496:CDS 40.0%
!!! ACCTTCCTTTAGTTTTTGCG+CGG - chr7.3:77301659-77301678 None:intergenic 40.0%
AACATTAGAAGGCCCATCCA+TGG - chr7.3:77301875-77301894 None:intergenic 45.0%
AGTGTGTTAAGTGCGATTGC+CGG + chr7.3:77300874-77300893 MS.gene92496:CDS 45.0%
CATAACGTCTCCGATTGCTT+TGG - chr7.3:77300560-77300579 None:intergenic 45.0%
CCAAGAACATGGCTTCAAGA+TGG + chr7.3:77299706-77299725 MS.gene92496:CDS 45.0%
CCATCTTGAAGCCATGTTCT+TGG - chr7.3:77299709-77299728 None:intergenic 45.0%
CCCATCCATGGAAGAACTAA+TGG - chr7.3:77301863-77301882 None:intergenic 45.0%
CTGATATTTATGATGCGGGG+AGG - chr7.3:77301003-77301022 None:intergenic 45.0%
GAACCAGAAGGAGAAATCGT+AGG + chr7.3:77299830-77299849 MS.gene92496:CDS 45.0%
GGTGCACCAAAAGAAGTAGA+AGG - chr7.3:77301928-77301947 None:intergenic 45.0%
TACAGATCAAGATGGAGCCA+TGG + chr7.3:77300534-77300553 MS.gene92496:CDS 45.0%
TCACCTACGATTTCTCCTTC+TGG - chr7.3:77299836-77299855 None:intergenic 45.0%
! TGGTCAAGTTGTGCATAGCA+AGG + chr7.3:77299664-77299683 MS.gene92496:CDS 45.0%
!! ATGGAGCACTGCATATAGCA+AGG + chr7.3:77300801-77300820 MS.gene92496:CDS 45.0%
ACAACTGATCGTTGCAGCAG+AGG - chr7.3:77301208-77301227 None:intergenic 50.0%
GAGTATCTTGCTGGAACTGG+TGG + chr7.3:77301084-77301103 MS.gene92496:CDS 50.0%
GCCGCGCAAAAACTAAAGGA+AGG + chr7.3:77301655-77301674 MS.gene92496:intron 50.0%
! ACTTAGACTGGTTGCTGCTC+CGG - chr7.3:77300896-77300915 None:intergenic 50.0%
CCTCGCCGCGCAAAAACTAA+AGG + chr7.3:77301651-77301670 MS.gene92496:intron 55.0%
GGAGCCATGGCCAAAGCAAT+CGG + chr7.3:77300547-77300566 MS.gene92496:CDS 55.0%
GTCTCCGATTGCTTTGGCCA+TGG - chr7.3:77300554-77300573 None:intergenic 55.0%
! TGATGCGGGGAGGCATACTT+TGG - chr7.3:77300993-77301012 None:intergenic 55.0%
!!! CCTTTAGTTTTTGCGCGGCG+AGG - chr7.3:77301654-77301673 None:intergenic 55.0%
GCTCGAGCGGCACCAAAGCA+TGG - chr7.3:77299967-77299986 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 77299608 77302275 77299608 ID=MS.gene92496
chr7.3 mRNA 77299608 77302275 77299608 ID=MS.gene92496.t1;Parent=MS.gene92496
chr7.3 exon 77299608 77301294 77299608 ID=MS.gene92496.t1.exon1;Parent=MS.gene92496.t1
chr7.3 CDS 77299608 77301294 77299608 ID=cds.MS.gene92496.t1;Parent=MS.gene92496.t1
chr7.3 exon 77301833 77301954 77301833 ID=MS.gene92496.t1.exon2;Parent=MS.gene92496.t1
chr7.3 CDS 77301833 77301954 77301833 ID=cds.MS.gene92496.t1;Parent=MS.gene92496.t1
chr7.3 exon 77302043 77302135 77302043 ID=MS.gene92496.t1.exon3;Parent=MS.gene92496.t1
chr7.3 CDS 77302043 77302135 77302043 ID=cds.MS.gene92496.t1;Parent=MS.gene92496.t1
chr7.3 exon 77302225 77302275 77302225 ID=MS.gene92496.t1.exon4;Parent=MS.gene92496.t1
chr7.3 CDS 77302225 77302275 77302225 ID=cds.MS.gene92496.t1;Parent=MS.gene92496.t1
Gene Sequence

>MS.gene92496

ATGGAGGATTCTGAATGTCAAGCAGTTGAAATTGACAAGCCCAGAGATATTTTTCTTGGTCAAGTTGTGCATAGCAAGGATGAAGCTTATAACTTATACCAAGAACATGGCTTCAAGATGGGTTTTAGTGTGAGAAAAGGGAAGGAATTGTACTATGATAATAATAAAAAGAACACACGTTTGAAAGATTATTATTGTTCCAAACAAGGGTTTAAGAACAATGAACCAGAAGGAGAAATCGTAGGTGAAGTTGCTTATCAAAGGGCTGATTCAAGGACAAATTGTAAAGCAATGGTGCGATTTAATGTTAGCAAGGATGGAGAATGGCGAATTACTAAGTTGATAATGGATCACAACCATGCTTTGGTGCCGCTCGAGCAAAGACATTTGTTACGCTCTATGAGAAAAGTATCAGAAACCAAAGGAGGTCTCATAAAGTCTATGGTGAATGCTGGTATGAAAGTTACTAATGTTTGGAGTTATTTAGGAGAAGAGGTTGGTGGATTTGATAAGCTTGGCATGACAATGAAAGATGTGCATAATTTTGTCTACACTGAAAAGAGTAAATTAATTAAAGGTGGAGATGCCCAATCTTTAATCAATCACTTGCAAAATAGACAAGCACAAGATGCCATGTTCTACTATAGTGTGCAGTTAGACCAAGAATCACGTTTAACCAATGTGTTTTGGAGAGATGGTAAATCCAAAGTTGATTATGATTGTTTTGGAGATGTTGTGGTTTTTGATACCACTTACCGTACTAATACATATGATTTGATATGTGCTCCTTTTGTTGGGGTAAATCATCATTTGCAAAATGTCATGTTTGGTTGTGCTTTGTTATCTGATGAAACTTCTGTTTCTTTCAATTGGCTATTTAAAGTGTTTTTGGAGAGCATGGGAAATAGACAACCAAAAACATTTTTTACAGATCAAGATGGAGCCATGGCCAAAGCAATCGGAGACGTTATGCCAAATACATGTCACCGTTTATGCTTATGGCATATATCTCAGAATGCACCTTCTCATTTTGGTGATCTTAACTCTGATCAAAAGTTTCATGGTTTGTTTTGGAAATGCATGTCAGGCTGTGATTCAAAAGAAGAGTTTGAAGGCACTTGGAGTTATATGATTAATGAGTACAAGCTTCAAAATCACAAATGGTTGAAAAGCATGTATAAAATTCGTCATAAATGGAGCACTGCATATAGCAAGGATGTGTTCTCTGCTGATATCAAGTCATCTCAAAGAAGTGAGAGTACAAATAGTGTGTTAAGTGCGATTGCCGGAGCAGCAACCAGTCTAAGTCATTTCTTATTAGCATTTGAAAAGATGGTAAACAAGTGGCGACAATTAGAGACTGAAAAGGAATTCAAAAATAGCCAAAGTATGCCTCCCCGCATCATAAATATCAGTGAGACTTTGAGACAAGCATCTATGATATACACACACAAGACTTTCAAACTTTTTTTGAATGAGTATCTTGCTGGAACTGGTGGAAGTACTTCTAAAGACATTGGGGAATCTGATAATTTGTCATATCATGAAGTGAGTTCAAATCATATGCCTAATAAGAAACATGTGGTAGTATTTGACTCCTCTGCTGCAACGATCAGTTGTAGTTGTCGAAAGTTTGATTCAATGGGCATTCTTTGTAGTCATGCTTTGCGAATTTACAACATCAAAGGTATATTAAAAATACCTGACCAATACTTTCTGAGAAGATGGTCAAAAAAAGCAAGGGATGTGATTTATGATCACATACATGAGAGTTCAAAAGAAAATTCTATCTCAGAAAATGCAATGAACAATGGTGGTGATTCCATGATCCTTTATCGCAATGCTATTATGAAGTTTTTTTATAATCTAGTTTTGGATAGCCAGGAACACAAAGAAACTCAACAAATCATGTGGAAATTGGTTTATGGTGGAGTTAAGAGTGTGCACCAATGCATCAATGAGCTCAATTTGATTTCAAAAGCAACAACCAGTGAAAAAAATTTCACTCAAAATGAGGATAGTGATCACATTGCAATTTATAATCCTCGCCGCGCAAAAACTAAAGGAAGGTCTAATACTCGCATGAAAGGGCATTTTGAAACAAAAAGGATCAACAACAAAAGAAAAGCAACATAAAAGTGAGTTTCTTGATTATTTTTTAAGTATACTTTCATCATTTATTGAACATGTTTAATAAATTGTTCTGTTAATATTTTTTATCACAGAGAAAAAAAAAGAAGATAGCTTTTGTTCCATTAGTTCTTCCATGGATGGGCCTTCTAATGTTCTCAATAACATGGACAACACTTATAATGTTCCTTCTACTTCTTTTGGTGCACCTTTAGAGGTTTATAATTTATCTCCATTTCTTGATAACTTTTAAGTTATGAATGATTTCATTGTGGCTAATAACCTTCTTTTACATAATTTAATAGGGTTATCATTCCTCAAATTGGGGTTTAAATACTCCATCCATTTCATTTACTAAGATGTTACAAGGAATTGGCACATGTAACTCATTGATTCAGGTATGTATGTATGTAGGTAATCAAAACATAACTTCATTCAAAACTTAAACATGTCTGATTGCTCAAGTATTCAGTTATATTGCATGCAGGAATCTCCAAGTAGTGCTTTCCAAGATAGGCAAGAACATAAAAGAAGTTAG
Protein sequence

>MS.gene92496.t1

MEDSECQAVEIDKPRDIFLGQVVHSKDEAYNLYQEHGFKMGFSVRKGKELYYDNNKKNTRLKDYYCSKQGFKNNEPEGEIVGEVAYQRADSRTNCKAMVRFNVSKDGEWRITKLIMDHNHALVPLEQRHLLRSMRKVSETKGGLIKSMVNAGMKVTNVWSYLGEEVGGFDKLGMTMKDVHNFVYTEKSKLIKGGDAQSLINHLQNRQAQDAMFYYSVQLDQESRLTNVFWRDGKSKVDYDCFGDVVVFDTTYRTNTYDLICAPFVGVNHHLQNVMFGCALLSDETSVSFNWLFKVFLESMGNRQPKTFFTDQDGAMAKAIGDVMPNTCHRLCLWHISQNAPSHFGDLNSDQKFHGLFWKCMSGCDSKEEFEGTWSYMINEYKLQNHKWLKSMYKIRHKWSTAYSKDVFSADIKSSQRSESTNSVLSAIAGAATSLSHFLLAFEKMVNKWRQLETEKEFKNSQSMPPRIINISETLRQASMIYTHKTFKLFLNEYLAGTGGSTSKDIGESDNLSYHEVSSNHMPNKKHVVVFDSSAATISCSCRKFDSMGILCSHALRIYNIKEKKKEDSFCSISSSMDGPSNVLNNMDNTYNVPSTSFGAPLEGYHSSNWGLNTPSISFTKMLQGIGTCNSLIQESPSSAFQDRQEHKRS