Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92501.t1 | AES77954.1 | 90 | 239 | 24 | 0 | 1 | 239 | 1 | 239 | 2.10E-117 | 431.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92501.t1 | Q39261 | 36.9 | 84 | 45 | 3 | 87 | 170 | 52 | 127 | 4.8e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92501.t1 | G7KUQ7 | 90.0 | 239 | 24 | 0 | 1 | 239 | 1 | 239 | 1.5e-117 | 431.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene92501.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92501.t1 | MTR_7g022250 | 89.958 | 239 | 24 | 0 | 1 | 239 | 1 | 239 | 2.02e-162 | 449 |
| MS.gene92501.t1 | MTR_7g028890 | 69.038 | 239 | 55 | 3 | 1 | 239 | 1 | 220 | 1.17e-116 | 332 |
| MS.gene92501.t1 | MTR_7g028915 | 68.201 | 239 | 49 | 3 | 1 | 239 | 1 | 212 | 9.40e-111 | 317 |
| MS.gene92501.t1 | MTR_7g022270 | 67.005 | 197 | 28 | 3 | 24 | 219 | 1 | 161 | 1.92e-87 | 256 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATGACTGAGATTGAATT+TGG | 0.189345 | 7.3:+77383747 | MS.gene92501:CDS |
| TTGAATTCTTAGTTTCAAAA+TGG | 0.214469 | 7.3:-77383275 | None:intergenic |
| CTTGAGTCTCATTGTCTTCC+TGG | 0.219987 | 7.3:-77383809 | None:intergenic |
| ATTCTTCATCATCTTCAAAT+TGG | 0.223485 | 7.3:-77383208 | None:intergenic |
| CTCCTTCCCCTTGTTCTCAT+TGG | 0.229008 | 7.3:-77383163 | None:intergenic |
| CAAATTTGTGAGAACTTTGA+TGG | 0.256763 | 7.3:-77383464 | None:intergenic |
| AAGAAATGGAGGAATTTCTT+TGG | 0.278435 | 7.3:-77383343 | None:intergenic |
| ACATATAGATGAAGTGAATA+AGG | 0.341762 | 7.3:+77383695 | MS.gene92501:CDS |
| CACTGCCACTCCTTTCCATA+TGG | 0.342482 | 7.3:+77383572 | MS.gene92501:CDS |
| AGAAATGGAGGAATTTCTTT+GGG | 0.353550 | 7.3:-77383342 | None:intergenic |
| CTTAAGGGTCAAATCAATCT+TGG | 0.361873 | 7.3:-77383844 | None:intergenic |
| AATATATAGATCAAAGCTTA+AGG | 0.366337 | 7.3:-77383860 | None:intergenic |
| TCCAAGCTTCGTTAAAAGCT+TGG | 0.373123 | 7.3:-77383512 | None:intergenic |
| AATTACTCAAATGGTAATCA+AGG | 0.414383 | 7.3:+77383537 | MS.gene92501:CDS |
| TGAATTCTTAGTTTCAAAAT+GGG | 0.419158 | 7.3:-77383274 | None:intergenic |
| ACTCCTTTCCATATGGGTTG+TGG | 0.424172 | 7.3:+77383579 | MS.gene92501:CDS |
| TTCACTACTCCACGTGCATT+AGG | 0.424339 | 7.3:+77383438 | MS.gene92501:CDS |
| AACAATATGATTCAGCATGC+AGG | 0.425567 | 7.3:+77383621 | MS.gene92501:CDS |
| ATTGATATCCACAACCCATA+TGG | 0.434960 | 7.3:-77383587 | None:intergenic |
| GCTTGGATAAATTACTCAAA+TGG | 0.446981 | 7.3:+77383528 | MS.gene92501:CDS |
| TCACAAATTTGAGAGAAGTC+TGG | 0.482572 | 7.3:+77383476 | MS.gene92501:CDS |
| ACTGCCACTCCTTTCCATAT+GGG | 0.486001 | 7.3:+77383573 | MS.gene92501:CDS |
| TTATTCACTTCATCTATATG+TGG | 0.494069 | 7.3:-77383693 | None:intergenic |
| TTAAGGGTCAAATCAATCTT+GGG | 0.500241 | 7.3:-77383843 | None:intergenic |
| ATATATAGATCAAAGCTTAA+GGG | 0.520066 | 7.3:-77383859 | None:intergenic |
| TGAATTTGGTTCAATTTCAG+AGG | 0.531449 | 7.3:+77383761 | MS.gene92501:CDS |
| TTGGATTCATTGTTGATCAG+AGG | 0.552579 | 7.3:+77383370 | MS.gene92501:CDS |
| GAAGTGAATAAGGCTGATCA+AGG | 0.555258 | 7.3:+77383705 | MS.gene92501:CDS |
| TTCATTGTTGATCAGAGGAA+AGG | 0.555662 | 7.3:+77383375 | MS.gene92501:CDS |
| ACAATATGATTCAGCATGCA+GGG | 0.561522 | 7.3:+77383622 | MS.gene92501:CDS |
| GTGCAACACCAATGAGAACA+AGG | 0.569603 | 7.3:+77383155 | MS.gene92501:CDS |
| CAAGCGAAAGGCCATCGTGA+TGG | 0.579476 | 7.3:+77383245 | MS.gene92501:CDS |
| GAATTTGGTTCAATTTCAGA+GGG | 0.586650 | 7.3:+77383762 | MS.gene92501:CDS |
| ATTACTCAAATGGTAATCAA+GGG | 0.589859 | 7.3:+77383538 | MS.gene92501:CDS |
| TGTTAGTGACAAAGTTGACC+AGG | 0.590985 | 7.3:+77383791 | MS.gene92501:CDS |
| TGAGAACAAGGGGAAGGAGA+AGG | 0.594646 | 7.3:+77383167 | MS.gene92501:CDS |
| TTTGGTTCAATTTCAGAGGG+AGG | 0.596815 | 7.3:+77383765 | MS.gene92501:CDS |
| CACCAATGAGAACAAGGGGA+AGG | 0.620535 | 7.3:+77383161 | MS.gene92501:CDS |
| TATCCACAACCCATATGGAA+AGG | 0.633718 | 7.3:-77383582 | None:intergenic |
| GGAAGACAATGAGACTCAAG+AGG | 0.638438 | 7.3:+77383812 | MS.gene92501:CDS |
| ACTACTCCACGTGCATTAGG+TGG | 0.640042 | 7.3:+77383441 | MS.gene92501:CDS |
| TCATCTATATGTGGAATATG+CGG | 0.642794 | 7.3:-77383684 | None:intergenic |
| GCAACACCAATGAGAACAAG+GGG | 0.642885 | 7.3:+77383157 | MS.gene92501:CDS |
| TGCAACACCAATGAGAACAA+GGG | 0.650590 | 7.3:+77383156 | MS.gene92501:CDS |
| GAACAATGTGAACAAGCGAA+AGG | 0.655169 | 7.3:+77383233 | MS.gene92501:CDS |
| ACAACCCATATGGAAAGGAG+TGG | 0.680303 | 7.3:-77383577 | None:intergenic |
| AGACAATGAGACTCAAGAGG+AGG | 0.680897 | 7.3:+77383815 | MS.gene92501:CDS |
| ATGGGATTCTTCCATCACGA+TGG | 0.687731 | 7.3:-77383256 | None:intergenic |
| TGATGGCCACCTAATGCACG+TGG | 0.714086 | 7.3:-77383447 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATTCTTAGTTTCAAAAT+GGG | - | chr7.3:77383277-77383296 | None:intergenic | 20.0% |
| !! | TTGAATTCTTAGTTTCAAAA+TGG | - | chr7.3:77383278-77383297 | None:intergenic | 20.0% |
| !!! | TTCTTCAACAATATCTTTTT+GGG | - | chr7.3:77383307-77383326 | None:intergenic | 20.0% |
| ! | AATATGACTGAGATTGAATT+TGG | + | chr7.3:77383747-77383766 | MS.gene92501:CDS | 25.0% |
| ! | AATTACTCAAATGGTAATCA+AGG | + | chr7.3:77383537-77383556 | MS.gene92501:CDS | 25.0% |
| ! | ACATATAGATGAAGTGAATA+AGG | + | chr7.3:77383695-77383714 | MS.gene92501:CDS | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | - | chr7.3:77383360-77383379 | None:intergenic | 25.0% |
| ! | ATTACTCAAATGGTAATCAA+GGG | + | chr7.3:77383538-77383557 | MS.gene92501:CDS | 25.0% |
| ! | ATTCTTCATCATCTTCAAAT+TGG | - | chr7.3:77383211-77383230 | None:intergenic | 25.0% |
| ! | TTATTCACTTCATCTATATG+TGG | - | chr7.3:77383696-77383715 | None:intergenic | 25.0% |
| !! | CTTCTTCAACAATATCTTTT+TGG | - | chr7.3:77383308-77383327 | None:intergenic | 25.0% |
| !! | TCTTCAACAATATCTTTTTG+GGG | - | chr7.3:77383306-77383325 | None:intergenic | 25.0% |
| !!! | TTTTATGCAGGAAATTTGTT+TGG | + | chr7.3:77383654-77383673 | MS.gene92501:CDS | 25.0% |
| AAGAAATGGAGGAATTTCTT+TGG | - | chr7.3:77383346-77383365 | None:intergenic | 30.0% | |
| AATCCAAAAAGAAGAAATGG+AGG | - | chr7.3:77383357-77383376 | None:intergenic | 30.0% | |
| AGAAATGGAGGAATTTCTTT+GGG | - | chr7.3:77383345-77383364 | None:intergenic | 30.0% | |
| CAAATTTGTGAGAACTTTGA+TGG | - | chr7.3:77383467-77383486 | None:intergenic | 30.0% | |
| GCTTGGATAAATTACTCAAA+TGG | + | chr7.3:77383528-77383547 | MS.gene92501:CDS | 30.0% | |
| TCATCTATATGTGGAATATG+CGG | - | chr7.3:77383687-77383706 | None:intergenic | 30.0% | |
| TTAAGGGTCAAATCAATCTT+GGG | - | chr7.3:77383846-77383865 | None:intergenic | 30.0% | |
| ! | ATTCCTCCATTTCTTCTTTT+TGG | + | chr7.3:77383351-77383370 | MS.gene92501:CDS | 30.0% |
| ! | GAATTTGGTTCAATTTCAGA+GGG | + | chr7.3:77383762-77383781 | MS.gene92501:CDS | 30.0% |
| ! | TGAATTTGGTTCAATTTCAG+AGG | + | chr7.3:77383761-77383780 | MS.gene92501:CDS | 30.0% |
| AACAATATGATTCAGCATGC+AGG | + | chr7.3:77383621-77383640 | MS.gene92501:CDS | 35.0% | |
| ACAATATGATTCAGCATGCA+GGG | + | chr7.3:77383622-77383641 | MS.gene92501:CDS | 35.0% | |
| AGAGAAGTCTGGTCAAAAAA+AGG | + | chr7.3:77383487-77383506 | MS.gene92501:CDS | 35.0% | |
| ATTGATATCCACAACCCATA+TGG | - | chr7.3:77383590-77383609 | None:intergenic | 35.0% | |
| CTTAAGGGTCAAATCAATCT+TGG | - | chr7.3:77383847-77383866 | None:intergenic | 35.0% | |
| TCACAAATTTGAGAGAAGTC+TGG | + | chr7.3:77383476-77383495 | MS.gene92501:CDS | 35.0% | |
| TTCATTGTTGATCAGAGGAA+AGG | + | chr7.3:77383375-77383394 | MS.gene92501:CDS | 35.0% | |
| TTGGATTCATTGTTGATCAG+AGG | + | chr7.3:77383370-77383389 | MS.gene92501:CDS | 35.0% | |
| GAACAATGTGAACAAGCGAA+AGG | + | chr7.3:77383233-77383252 | MS.gene92501:CDS | 40.0% | |
| GAAGTGAATAAGGCTGATCA+AGG | + | chr7.3:77383705-77383724 | MS.gene92501:CDS | 40.0% | |
| TATCCACAACCCATATGGAA+AGG | - | chr7.3:77383585-77383604 | None:intergenic | 40.0% | |
| TCCAAGCTTCGTTAAAAGCT+TGG | - | chr7.3:77383515-77383534 | None:intergenic | 40.0% | |
| TGCAACACCAATGAGAACAA+GGG | + | chr7.3:77383156-77383175 | MS.gene92501:CDS | 40.0% | |
| TGTTAGTGACAAAGTTGACC+AGG | + | chr7.3:77383791-77383810 | MS.gene92501:CDS | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | + | chr7.3:77383642-77383661 | MS.gene92501:CDS | 40.0% |
| ! | TCCAAGCTTTTAACGAAGCT+TGG | + | chr7.3:77383511-77383530 | MS.gene92501:CDS | 40.0% |
| ! | TTTGGTTCAATTTCAGAGGG+AGG | + | chr7.3:77383765-77383784 | MS.gene92501:CDS | 40.0% |
| ACAACCCATATGGAAAGGAG+TGG | - | chr7.3:77383580-77383599 | None:intergenic | 45.0% | |
| ACTGCCACTCCTTTCCATAT+GGG | + | chr7.3:77383573-77383592 | MS.gene92501:CDS | 45.0% | |
| AGACAATGAGACTCAAGAGG+AGG | + | chr7.3:77383815-77383834 | MS.gene92501:CDS | 45.0% | |
| ATGGGATTCTTCCATCACGA+TGG | - | chr7.3:77383259-77383278 | None:intergenic | 45.0% | |
| CTTGAGTCTCATTGTCTTCC+TGG | - | chr7.3:77383812-77383831 | None:intergenic | 45.0% | |
| GCAACACCAATGAGAACAAG+GGG | + | chr7.3:77383157-77383176 | MS.gene92501:CDS | 45.0% | |
| GGAAGACAATGAGACTCAAG+AGG | + | chr7.3:77383812-77383831 | MS.gene92501:CDS | 45.0% | |
| GTGCAACACCAATGAGAACA+AGG | + | chr7.3:77383155-77383174 | MS.gene92501:CDS | 45.0% | |
| TTCACTACTCCACGTGCATT+AGG | + | chr7.3:77383438-77383457 | MS.gene92501:CDS | 45.0% | |
| ! | ACTCCTTTCCATATGGGTTG+TGG | + | chr7.3:77383579-77383598 | MS.gene92501:CDS | 45.0% |
| ACTACTCCACGTGCATTAGG+TGG | + | chr7.3:77383441-77383460 | MS.gene92501:CDS | 50.0% | |
| CACCAATGAGAACAAGGGGA+AGG | + | chr7.3:77383161-77383180 | MS.gene92501:CDS | 50.0% | |
| CACTGCCACTCCTTTCCATA+TGG | + | chr7.3:77383572-77383591 | MS.gene92501:CDS | 50.0% | |
| CTCCTTCCCCTTGTTCTCAT+TGG | - | chr7.3:77383166-77383185 | None:intergenic | 50.0% | |
| TGAGAACAAGGGGAAGGAGA+AGG | + | chr7.3:77383167-77383186 | MS.gene92501:CDS | 50.0% | |
| CAAGCGAAAGGCCATCGTGA+TGG | + | chr7.3:77383245-77383264 | MS.gene92501:CDS | 55.0% | |
| TGATGGCCACCTAATGCACG+TGG | - | chr7.3:77383450-77383469 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 77383153 | 77383872 | 77383153 | ID=MS.gene92501 |
| chr7.3 | mRNA | 77383153 | 77383872 | 77383153 | ID=MS.gene92501.t1;Parent=MS.gene92501 |
| chr7.3 | exon | 77383153 | 77383872 | 77383153 | ID=MS.gene92501.t1.exon1;Parent=MS.gene92501.t1 |
| chr7.3 | CDS | 77383153 | 77383872 | 77383153 | ID=cds.MS.gene92501.t1;Parent=MS.gene92501.t1 |
| Gene Sequence |
| Protein sequence |
