Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92836.t1 | GAU37750.1 | 34.4 | 221 | 130 | 2 | 5 | 224 | 3 | 209 | 2.40E-30 | 142.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92836.t1 | A0A2Z6NMK1 | 34.4 | 221 | 130 | 2 | 5 | 224 | 3 | 209 | 1.7e-30 | 142.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene92836.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92836.t1 | MTR_4g123470 | 27.461 | 193 | 140 | 0 | 17 | 209 | 18 | 210 | 5.02e-23 | 93.2 |
| MS.gene92836.t1 | MTR_1g054495 | 23.204 | 181 | 138 | 1 | 30 | 210 | 28 | 207 | 1.87e-13 | 67.4 |
| MS.gene92836.t1 | MTR_0014s0220 | 24.599 | 187 | 124 | 3 | 28 | 214 | 644 | 813 | 7.28e-13 | 67.8 |
| MS.gene92836.t1 | MTR_4g085790 | 24.468 | 188 | 141 | 1 | 28 | 215 | 26 | 212 | 8.85e-13 | 65.5 |
| MS.gene92836.t1 | MTR_1g090753 | 34.091 | 88 | 58 | 0 | 30 | 117 | 45 | 132 | 1.04e-12 | 64.3 |
| MS.gene92836.t1 | MTR_1g054485 | 23.952 | 167 | 127 | 0 | 23 | 189 | 21 | 187 | 1.57e-12 | 64.3 |
| MS.gene92836.t1 | MTR_4g053550 | 42.857 | 70 | 40 | 0 | 28 | 97 | 49 | 118 | 2.70e-12 | 62.0 |
| MS.gene92836.t1 | MTR_7g025150 | 23.656 | 186 | 141 | 1 | 30 | 215 | 28 | 212 | 4.09e-12 | 63.5 |
| MS.gene92836.t1 | MTR_5g074790 | 30.769 | 104 | 72 | 0 | 15 | 118 | 160 | 263 | 1.94e-11 | 62.4 |
| MS.gene92836.t1 | MTR_1g041870 | 23.626 | 182 | 112 | 2 | 30 | 211 | 7 | 161 | 8.09e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATGTGATGTACTAATA+AGG | 0.256110 | 3.4:+21471242 | MS.gene92836:CDS |
| AATGATCTTTATGAAGTTCT+TGG | 0.265359 | 3.4:+21470907 | MS.gene92836:CDS |
| TAGATGTAATGAAGGTTTCT+AGG | 0.282714 | 3.4:+21471377 | MS.gene92836:CDS |
| ATGATCTTTATGAAGTTCTT+GGG | 0.324200 | 3.4:+21470908 | MS.gene92836:CDS |
| AATGACCTTAACTCCTTATA+TGG | 0.332349 | 3.4:+21471036 | MS.gene92836:CDS |
| TAAATTCTCTTCCAAGTTTG+TGG | 0.333428 | 3.4:-21470766 | None:intergenic |
| TGATAACCTGTAGCCATATA+AGG | 0.345028 | 3.4:-21471049 | None:intergenic |
| CTGAGGGATGGATCAACTTA+AGG | 0.380199 | 3.4:+21470858 | MS.gene92836:CDS |
| AGGACTAAGCATAGGGATAA+TGG | 0.384187 | 3.4:+21470592 | MS.gene92836:CDS |
| GTCCACGAAATGCAGTGAAT+TGG | 0.385092 | 3.4:-21470943 | None:intergenic |
| TAAGCTTAAGTTTAAGTTTG+TGG | 0.399998 | 3.4:+21471342 | MS.gene92836:CDS |
| TTCCAATTCACTGCATTTCG+TGG | 0.438395 | 3.4:+21470941 | MS.gene92836:CDS |
| CAAATGTGATGTACTAATAA+GGG | 0.442101 | 3.4:+21471243 | MS.gene92836:CDS |
| GAGTTAAGGTCATTTCATAA+TGG | 0.446526 | 3.4:-21471027 | None:intergenic |
| TGTAGCCATATAAGGAGTTA+AGG | 0.450536 | 3.4:-21471041 | None:intergenic |
| GTTGTTATCAATACAATGTC+TGG | 0.455307 | 3.4:+21470553 | None:intergenic |
| GTTGATTACATTACAAGCTC+AGG | 0.465507 | 3.4:+21471178 | MS.gene92836:CDS |
| TATGGCTACAGGTTATCAAT+TGG | 0.468564 | 3.4:+21471054 | MS.gene92836:CDS |
| ATTAATATGTATCGACCTAC+AGG | 0.482580 | 3.4:+21471211 | MS.gene92836:CDS |
| TGAGGGATGGATCAACTTAA+GGG | 0.485967 | 3.4:+21470859 | MS.gene92836:CDS |
| CTGGTGGTGGATCAACTTCA+AGG | 0.486585 | 3.4:+21470572 | MS.gene92836:CDS |
| GAGCATTGTATCCACAAACT+TGG | 0.499256 | 3.4:+21470755 | MS.gene92836:CDS |
| TTAACTCCTTATATGGCTAC+AGG | 0.499584 | 3.4:+21471043 | MS.gene92836:CDS |
| AAGTACATTAGATGTAATGA+AGG | 0.505287 | 3.4:+21471369 | MS.gene92836:CDS |
| ATGTTAACTCATTGCCATCA+TGG | 0.509173 | 3.4:+21470972 | MS.gene92836:CDS |
| CACTTACAAGCAAGGAATTG+AGG | 0.515012 | 3.4:+21470997 | MS.gene92836:CDS |
| ATACAATGCTCAACAAATGA+AGG | 0.516993 | 3.4:-21470743 | None:intergenic |
| ATCCATCCCTCAGTCAGAAG+TGG | 0.551673 | 3.4:-21470848 | None:intergenic |
| AAGACAATGGATTCCGTGGT+GGG | 0.557747 | 3.4:+21471317 | MS.gene92836:CDS |
| ATCAATACAATGTCTGGTGG+TGG | 0.560856 | 3.4:+21470559 | None:intergenic |
| TGATCTTTATGAAGTTCTTG+GGG | 0.561506 | 3.4:+21470909 | MS.gene92836:CDS |
| CAACTTCAAGGACTAAGCAT+AGG | 0.561912 | 3.4:+21470584 | MS.gene92836:CDS |
| AACTTCAAGGACTAAGCATA+GGG | 0.565650 | 3.4:+21470585 | MS.gene92836:CDS |
| TGCTTGTAAGTGTGCCATGA+TGG | 0.567390 | 3.4:-21470986 | None:intergenic |
| ATCCACTTCTGACTGAGGGA+TGG | 0.571896 | 3.4:+21470846 | MS.gene92836:CDS |
| AAGGCAACAAAGATTGGCAG+TGG | 0.574230 | 3.4:+21471277 | MS.gene92836:CDS |
| TGTAAAGACAATGGATTCCG+TGG | 0.586483 | 3.4:+21471313 | MS.gene92836:CDS |
| CATTTGTACTCGGTGCCTGT+AGG | 0.588159 | 3.4:-21471226 | None:intergenic |
| TAGTACATCACATTTGTACT+CGG | 0.590498 | 3.4:-21471236 | None:intergenic |
| TGTGATGTACTAATAAGGGA+TGG | 0.597036 | 3.4:+21471247 | MS.gene92836:CDS |
| TGAACATCCACTTCTGACTG+AGG | 0.599795 | 3.4:+21470841 | MS.gene92836:CDS |
| CAACAAAGATTGGCAGTGGG+TGG | 0.601376 | 3.4:+21471281 | MS.gene92836:CDS |
| ATATCTCATTTATAGCCGCA+TGG | 0.613172 | 3.4:+21470710 | MS.gene92836:intron |
| AGACAATGGATTCCGTGGTG+GGG | 0.618908 | 3.4:+21471318 | MS.gene92836:CDS |
| TCATGGCACACTTACAAGCA+AGG | 0.641070 | 3.4:+21470989 | MS.gene92836:CDS |
| GAACATCCACTTCTGACTGA+GGG | 0.646274 | 3.4:+21470842 | MS.gene92836:CDS |
| AAAGACAATGGATTCCGTGG+TGG | 0.649549 | 3.4:+21471316 | MS.gene92836:CDS |
| AGGCAACAAAGATTGGCAGT+GGG | 0.670518 | 3.4:+21471278 | MS.gene92836:CDS |
| GTTATCAATACAATGTCTGG+TGG | 0.684260 | 3.4:+21470556 | None:intergenic |
| ACTTAAGCTTATCCCCACCA+CGG | 0.692884 | 3.4:-21471330 | None:intergenic |
| GAAGGTCTGATCTCTCCATG+CGG | 0.750032 | 3.4:-21470725 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGCTCAACATAAAATAAT+AGG | - | chr3.4:21470659-21470678 | None:intergenic | 20.0% |
| !! | TAGAGATGTATCATTTAATA+AGG | - | chr3.4:21471095-21471114 | None:intergenic | 20.0% |
| !!! | AACAAATTTTGTAAAGACAA+TGG | + | chr3.4:21471304-21471323 | MS.gene92836:CDS | 20.0% |
| ! | AAGTACATTAGATGTAATGA+AGG | + | chr3.4:21471369-21471388 | MS.gene92836:CDS | 25.0% |
| ! | AATGATCTTTATGAAGTTCT+TGG | + | chr3.4:21470907-21470926 | MS.gene92836:CDS | 25.0% |
| ! | ACAAATGTGATGTACTAATA+AGG | + | chr3.4:21471242-21471261 | MS.gene92836:CDS | 25.0% |
| ! | ATCATTTAATAAGGAGTTGT+TGG | - | chr3.4:21471086-21471105 | None:intergenic | 25.0% |
| ! | ATGATCTTTATGAAGTTCTT+GGG | + | chr3.4:21470908-21470927 | MS.gene92836:CDS | 25.0% |
| ! | CAAATGTGATGTACTAATAA+GGG | + | chr3.4:21471243-21471262 | MS.gene92836:CDS | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | + | chr3.4:21471342-21471361 | MS.gene92836:CDS | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | + | chr3.4:21471271-21471290 | MS.gene92836:CDS | 30.0% | |
| AATAAGGGATGGAAAAAAGA+AGG | + | chr3.4:21471258-21471277 | MS.gene92836:CDS | 30.0% | |
| AATGACCTTAACTCCTTATA+TGG | + | chr3.4:21471036-21471055 | MS.gene92836:CDS | 30.0% | |
| ATACAATGCTCAACAAATGA+AGG | - | chr3.4:21470746-21470765 | None:intergenic | 30.0% | |
| GAGTTAAGGTCATTTCATAA+TGG | - | chr3.4:21471030-21471049 | None:intergenic | 30.0% | |
| TAAATTCTCTTCCAAGTTTG+TGG | - | chr3.4:21470769-21470788 | None:intergenic | 30.0% | |
| TAGATGTAATGAAGGTTTCT+AGG | + | chr3.4:21471377-21471396 | MS.gene92836:CDS | 30.0% | |
| TAGTACATCACATTTGTACT+CGG | - | chr3.4:21471239-21471258 | None:intergenic | 30.0% | |
| TGATCTTTATGAAGTTCTTG+GGG | + | chr3.4:21470909-21470928 | MS.gene92836:CDS | 30.0% | |
| ! | ATCAACTTAAGGGAGTTTTA+TGG | + | chr3.4:21470869-21470888 | MS.gene92836:CDS | 30.0% |
| ! | ATTAATATGTATCGACCTAC+AGG | + | chr3.4:21471211-21471230 | MS.gene92836:CDS | 30.0% |
| AACTTCAAGGACTAAGCATA+GGG | + | chr3.4:21470585-21470604 | MS.gene92836:CDS | 35.0% | |
| ATATCTCATTTATAGCCGCA+TGG | + | chr3.4:21470710-21470729 | MS.gene92836:intron | 35.0% | |
| ATGTTAACTCATTGCCATCA+TGG | + | chr3.4:21470972-21470991 | MS.gene92836:CDS | 35.0% | |
| GTTGATTACATTACAAGCTC+AGG | + | chr3.4:21471178-21471197 | MS.gene92836:CDS | 35.0% | |
| TATGGCTACAGGTTATCAAT+TGG | + | chr3.4:21471054-21471073 | MS.gene92836:CDS | 35.0% | |
| TGATAACCTGTAGCCATATA+AGG | - | chr3.4:21471052-21471071 | None:intergenic | 35.0% | |
| TGTAGCCATATAAGGAGTTA+AGG | - | chr3.4:21471044-21471063 | None:intergenic | 35.0% | |
| TGTGATGTACTAATAAGGGA+TGG | + | chr3.4:21471247-21471266 | MS.gene92836:CDS | 35.0% | |
| TTAACTCCTTATATGGCTAC+AGG | + | chr3.4:21471043-21471062 | MS.gene92836:CDS | 35.0% | |
| AGGACTAAGCATAGGGATAA+TGG | + | chr3.4:21470592-21470611 | MS.gene92836:CDS | 40.0% | |
| CAACTTCAAGGACTAAGCAT+AGG | + | chr3.4:21470584-21470603 | MS.gene92836:CDS | 40.0% | |
| CACTTACAAGCAAGGAATTG+AGG | + | chr3.4:21470997-21471016 | MS.gene92836:CDS | 40.0% | |
| TGAGGGATGGATCAACTTAA+GGG | + | chr3.4:21470859-21470878 | MS.gene92836:CDS | 40.0% | |
| TTCCAATTCACTGCATTTCG+TGG | + | chr3.4:21470941-21470960 | MS.gene92836:CDS | 40.0% | |
| ! | GAGCATTGTATCCACAAACT+TGG | + | chr3.4:21470755-21470774 | MS.gene92836:CDS | 40.0% |
| ! | TGTAAAGACAATGGATTCCG+TGG | + | chr3.4:21471313-21471332 | MS.gene92836:CDS | 40.0% |
| AAGGCAACAAAGATTGGCAG+TGG | + | chr3.4:21471277-21471296 | MS.gene92836:CDS | 45.0% | |
| ACTTAAGCTTATCCCCACCA+CGG | - | chr3.4:21471333-21471352 | None:intergenic | 45.0% | |
| AGGCAACAAAGATTGGCAGT+GGG | + | chr3.4:21471278-21471297 | MS.gene92836:CDS | 45.0% | |
| CTGAGGGATGGATCAACTTA+AGG | + | chr3.4:21470858-21470877 | MS.gene92836:CDS | 45.0% | |
| GAACATCCACTTCTGACTGA+GGG | + | chr3.4:21470842-21470861 | MS.gene92836:CDS | 45.0% | |
| GTCCACGAAATGCAGTGAAT+TGG | - | chr3.4:21470946-21470965 | None:intergenic | 45.0% | |
| TCATGGCACACTTACAAGCA+AGG | + | chr3.4:21470989-21471008 | MS.gene92836:CDS | 45.0% | |
| TGAACATCCACTTCTGACTG+AGG | + | chr3.4:21470841-21470860 | MS.gene92836:CDS | 45.0% | |
| TGCTTGTAAGTGTGCCATGA+TGG | - | chr3.4:21470989-21471008 | None:intergenic | 45.0% | |
| ! | AAAGACAATGGATTCCGTGG+TGG | + | chr3.4:21471316-21471335 | MS.gene92836:CDS | 45.0% |
| ! | AAGACAATGGATTCCGTGGT+GGG | + | chr3.4:21471317-21471336 | MS.gene92836:CDS | 45.0% |
| ATCCACTTCTGACTGAGGGA+TGG | + | chr3.4:21470846-21470865 | MS.gene92836:CDS | 50.0% | |
| ATCCATCCCTCAGTCAGAAG+TGG | - | chr3.4:21470851-21470870 | None:intergenic | 50.0% | |
| CAACAAAGATTGGCAGTGGG+TGG | + | chr3.4:21471281-21471300 | MS.gene92836:CDS | 50.0% | |
| GAAGGTCTGATCTCTCCATG+CGG | - | chr3.4:21470728-21470747 | None:intergenic | 50.0% | |
| ! | AGACAATGGATTCCGTGGTG+GGG | + | chr3.4:21471318-21471337 | MS.gene92836:CDS | 50.0% |
| !! | CATTTGTACTCGGTGCCTGT+AGG | - | chr3.4:21471229-21471248 | None:intergenic | 50.0% |
| !! | CTGGTGGTGGATCAACTTCA+AGG | + | chr3.4:21470572-21470591 | MS.gene92836:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 21470568 | 21471405 | 21470568 | ID=MS.gene92836 |
| chr3.4 | mRNA | 21470568 | 21471405 | 21470568 | ID=MS.gene92836.t1;Parent=MS.gene92836 |
| chr3.4 | exon | 21470568 | 21470645 | 21470568 | ID=MS.gene92836.t1.exon1;Parent=MS.gene92836.t1 |
| chr3.4 | CDS | 21470568 | 21470645 | 21470568 | ID=cds.MS.gene92836.t1;Parent=MS.gene92836.t1 |
| chr3.4 | exon | 21470725 | 21471075 | 21470725 | ID=MS.gene92836.t1.exon2;Parent=MS.gene92836.t1 |
| chr3.4 | CDS | 21470725 | 21471075 | 21470725 | ID=cds.MS.gene92836.t1;Parent=MS.gene92836.t1 |
| chr3.4 | exon | 21471160 | 21471405 | 21471160 | ID=MS.gene92836.t1.exon3;Parent=MS.gene92836.t1 |
| chr3.4 | CDS | 21471160 | 21471405 | 21471160 | ID=cds.MS.gene92836.t1;Parent=MS.gene92836.t1 |
| Gene Sequence |
| Protein sequence |
