AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92844

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92844.t1 MTR_3g028520 94.891 411 16 1 1 411 1 406 0.0 807
MS.gene92844.t1 MTR_3g027650 94.891 411 16 1 1 411 1 406 0.0 807
MS.gene92844.t1 MTR_3g027650 91.198 409 27 3 1 408 1 401 0.0 756
MS.gene92844.t1 MTR_3g027650 94.521 365 15 1 1 365 1 360 0.0 707
MS.gene92844.t1 MTR_8g036235 51.111 405 153 10 10 401 18 390 1.00e-107 323
MS.gene92844.t1 MTR_8g036235 49.322 369 142 10 10 365 18 354 1.03e-86 269
MS.gene92844.t1 MTR_1g052470 37.108 415 187 17 14 399 1 370 1.38e-53 183
MS.gene92844.t1 MTR_2g098310 36.468 436 201 19 20 405 1 410 2.40e-53 183
MS.gene92844.t1 MTR_2g098310 36.256 422 193 19 20 391 1 396 7.66e-48 168
MS.gene92844.t1 MTR_4g065870 33.565 432 209 16 30 406 25 433 2.07e-47 168
MS.gene92844.t1 MTR_2g098310 33.758 471 201 20 20 405 1 445 6.26e-46 164
MS.gene92844.t1 MTR_4g065870 33.019 424 187 15 30 388 25 416 4.92e-42 153
MS.gene92844.t1 MTR_7g092540 37.450 251 113 6 166 399 92 315 1.81e-40 146
MS.gene92844.t1 MTR_7g092540 38.017 242 106 6 166 390 92 306 5.24e-38 139
MS.gene92844.t1 MTR_4g033295 67.033 91 28 1 313 403 106 194 2.44e-34 126
MS.gene92844.t1 MTR_2g098310 34.005 397 184 19 20 365 1 370 3.57e-32 126
MS.gene92844.t1 MTR_1g116790 60.577 104 40 1 297 400 236 338 3.51e-31 122
MS.gene92844.t1 MTR_4g033295 71.795 78 20 1 313 390 106 181 3.70e-31 117
MS.gene92844.t1 MTR_4g065870 30.175 401 183 15 30 365 25 393 1.03e-27 114
MS.gene92844.t1 MTR_1g052470 33.333 360 166 17 14 344 1 315 4.21e-26 107
MS.gene92844.t1 MTR_2g098310 32.447 376 176 19 20 344 1 349 6.82e-22 96.7
MS.gene92844.t1 MTR_4g033295 79.245 53 9 1 313 365 106 156 3.04e-20 88.2
MS.gene92844.t1 MTR_6g052280 51.190 84 33 2 290 365 9 92 5.48e-17 77.4
MS.gene92844.t1 MTR_4g131160 54.545 66 29 1 326 391 195 259 5.84e-15 76.6
MS.gene92844.t1 MTR_4g131160 54.545 66 29 1 326 391 198 262 6.15e-15 76.3
MS.gene92844.t1 MTR_3g498825 53.846 65 30 0 327 391 147 211 5.10e-14 73.2
MS.gene92844.t1 MTR_1g080890 53.030 66 30 1 326 391 192 256 4.55e-13 70.5
MS.gene92844.t1 MTR_8g012290 50.769 65 32 0 327 391 291 355 5.06e-13 70.9
MS.gene92844.t1 MTR_7g053410 50.769 65 32 0 327 391 317 381 5.07e-13 70.9
MS.gene92844.t1 MTR_8g012290 50.769 65 32 0 327 391 291 355 5.21e-13 70.9
MS.gene92844.t1 MTR_1g017350 47.761 67 35 0 325 391 258 324 5.21e-13 70.5
MS.gene92844.t1 MTR_3g116770 45.946 74 40 0 318 391 182 255 5.40e-13 70.5
MS.gene92844.t1 MTR_1g017350 47.761 67 35 0 325 391 258 324 5.46e-13 70.5
MS.gene92844.t1 MTR_3g116770 45.946 74 40 0 318 391 182 255 5.52e-13 70.5
MS.gene92844.t1 MTR_7g053410 50.769 65 32 0 327 391 317 381 5.57e-13 70.9
MS.gene92844.t1 MTR_1g017350 47.761 67 35 0 325 391 258 324 5.60e-13 70.5
MS.gene92844.t1 MTR_1g017350 47.761 67 35 0 325 391 258 324 6.02e-13 70.5
MS.gene92844.t1 MTR_8g065740 50.769 65 32 0 327 391 305 369 7.08e-13 70.5
MS.gene92844.t1 MTR_4g070320 47.368 76 38 1 318 391 132 207 1.36e-12 68.9
MS.gene92844.t1 MTR_5g048860 43.011 93 48 1 304 391 337 429 2.71e-12 68.9
MS.gene92844.t1 MTR_8g099880 49.231 65 33 0 327 391 145 209 4.60e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92844.t1 AT2G42280 34.872 390 187 14 34 405 17 357 2.58e-47 166
MS.gene92844.t1 AT1G51140 31.980 394 231 12 19 401 11 378 1.20e-44 159
MS.gene92844.t1 AT2G42280 33.957 374 180 14 34 389 17 341 2.47e-40 147
MS.gene92844.t1 AT1G05805 34.949 392 184 17 32 404 21 360 1.34e-38 142
MS.gene92844.t1 AT2G43140 50.336 149 59 4 259 405 77 212 1.99e-38 138
MS.gene92844.t1 AT2G43140 50.676 148 60 4 259 405 174 309 1.43e-37 138
MS.gene92844.t1 AT1G35460 67.857 84 27 0 322 405 176 259 1.50e-33 126
MS.gene92844.t1 AT4G09180 50.000 140 58 3 268 405 133 262 1.69e-32 124
MS.gene92844.t1 AT1G35460 72.000 50 14 0 319 368 173 222 4.62e-17 80.5
MS.gene92844.t1 AT2G42280 29.179 329 166 14 34 344 17 296 8.98e-17 80.9
MS.gene92844.t1 AT2G24260 56.061 66 28 1 326 391 34 98 1.61e-15 76.3
MS.gene92844.t1 AT2G24260 56.061 66 28 1 326 391 137 201 5.36e-15 76.3
MS.gene92844.t1 AT5G58010 53.030 66 30 1 326 391 98 162 7.49e-15 75.1
MS.gene92844.t1 AT4G30980 54.545 66 29 1 326 391 129 193 1.14e-14 74.7
MS.gene92844.t1 AT4G30980 54.545 66 29 1 326 391 177 241 1.42e-14 75.1
MS.gene92844.t1 AT4G34530 47.297 74 39 0 318 391 163 236 8.58e-14 72.4
MS.gene92844.t1 AT5G50915 50.769 65 32 0 327 391 136 200 2.91e-13 70.5
MS.gene92844.t1 AT5G50915 50.769 65 32 0 327 391 136 200 2.91e-13 70.5
MS.gene92844.t1 AT5G50915 50.769 65 32 0 327 391 136 200 3.61e-13 69.7
MS.gene92844.t1 AT5G50915 50.769 65 32 0 327 391 136 200 3.61e-13 69.7
MS.gene92844.t1 AT1G25330 47.692 65 34 0 327 391 104 168 5.59e-13 68.6
MS.gene92844.t1 AT1G68920 50.769 65 32 0 327 391 302 366 7.39e-13 70.5
MS.gene92844.t1 AT1G68920 50.769 65 32 0 327 391 303 367 7.68e-13 70.5
MS.gene92844.t1 AT1G68920 50.769 65 32 0 327 391 303 367 7.68e-13 70.5
MS.gene92844.t1 AT1G68920 50.769 65 32 0 327 391 303 367 7.68e-13 70.5
MS.gene92844.t1 AT3G07340 50.769 65 32 0 327 391 258 322 1.28e-12 69.7
MS.gene92844.t1 AT3G07340 50.769 65 32 0 327 391 258 322 1.40e-12 69.3
MS.gene92844.t1 AT1G26260 50.769 65 32 0 327 391 201 265 1.57e-12 68.9
MS.gene92844.t1 AT1G26260 50.769 65 32 0 327 391 223 287 1.65e-12 68.9
MS.gene92844.t1 AT1G26260 50.769 65 32 0 327 391 223 287 1.65e-12 68.9
MS.gene92844.t1 AT1G26260 50.769 65 32 0 327 391 223 287 1.65e-12 68.9
MS.gene92844.t1 AT1G26260 50.769 65 32 0 327 391 223 287 1.65e-12 68.9
MS.gene92844.t1 AT1G26260 50.769 65 32 0 327 391 172 236 1.70e-12 68.6
MS.gene92844.t1 AT1G10120 49.231 65 33 0 327 391 206 270 2.86e-12 68.2
MS.gene92844.t1 AT1G10120 49.231 65 33 0 327 391 206 270 2.86e-12 68.2
MS.gene92844.t1 AT4G36540 42.683 82 47 0 310 391 124 205 3.26e-12 67.4
MS.gene92844.t1 AT4G36540 42.683 82 47 0 310 391 124 205 3.54e-12 67.4
MS.gene92844.t1 AT5G48560 49.231 65 33 0 327 391 301 365 3.69e-12 68.2
MS.gene92844.t1 AT5G48560 49.231 65 33 0 327 391 301 365 4.21e-12 68.2
MS.gene92844.t1 AT1G73830 47.692 65 34 0 327 391 147 211 1.18e-11 65.1
MS.gene92844.t1 AT1G73830 47.692 65 34 0 327 391 147 211 1.28e-11 65.1
MS.gene92844.t1 AT2G18300 46.154 65 35 0 327 391 185 249 6.44e-11 63.9
MS.gene92844.t1 AT2G18300 46.154 65 35 0 327 391 185 249 6.82e-11 63.5
MS.gene92844.t1 AT2G18300 46.154 65 35 0 327 391 185 249 7.61e-11 63.5

Find 57 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAATACCTTATCTGATTT+TGG 0.030579 3.4:+21218016 None:intergenic
CTAGCTTCACCAATGAATTT+TGG 0.135326 3.4:-21218443 MS.gene92844:CDS
CTTGATTCTGTCACTAATTC+TGG 0.137372 3.4:+21218057 None:intergenic
ACCCTCTCTGCAATGCTTCT+TGG 0.180344 3.4:+21218189 None:intergenic
GACTCTCAAGAAGCAGATTT+TGG 0.193647 3.4:-21218348 MS.gene92844:CDS
GTACCTGAACTTGTTCTTGT+AGG 0.262088 3.4:+21217630 None:intergenic
TACTTGAAGAATCCATTTCT+TGG 0.275479 3.4:+21219960 None:intergenic
CAGAATGGTAATTATTCTTA+TGG 0.286535 3.4:-21219647 MS.gene92844:CDS
CACTATCAACAACAAAATTC+TGG 0.292913 3.4:-21219920 MS.gene92844:CDS
GATTCCAATCAATCTAAAAT+TGG 0.297971 3.4:-21219533 MS.gene92844:CDS
ATGCCACAAATAGCTGAAAA+TGG 0.316152 3.4:-21218543 MS.gene92844:CDS
TTTGAGCGTTGTTGTCGATT+AGG 0.323950 3.4:+21219852 None:intergenic
CACGGCAGATATGTTGGATT+TGG 0.326350 3.4:-21217672 MS.gene92844:CDS
TTTGTAGTTTGGTCACAATT+TGG 0.335830 3.4:+21218504 None:intergenic
ACAAACCTAGTAAATGAAAA+TGG 0.344072 3.4:-21218486 MS.gene92844:CDS
GGAATTGCTCCAATCTTATT+AGG 0.352113 3.4:-21219505 MS.gene92844:CDS
CATTTACTAGGTTTGTAGTT+TGG 0.361798 3.4:+21218493 None:intergenic
GAAGCTAGGTATGTGACTTT+TGG 0.365896 3.4:+21218458 None:intergenic
GCAGATACTCACAGATTGTA+AGG 0.393985 3.4:-21217520 MS.gene92844:intron
TGTAAAATTCGAGCGAAAAG+AGG 0.401428 3.4:-21218225 MS.gene92844:CDS
TCCATTTCTGAACTTGTTGA+TGG 0.404102 3.4:+21219785 None:intergenic
TCCATCAACAAGTTCAGAAA+TGG 0.409930 3.4:-21219786 MS.gene92844:CDS
AGAATTGTTCCATCCATTGT+TGG 0.420159 3.4:+21219736 None:intergenic
AGAATTGTCCCAAAATTCAT+TGG 0.420165 3.4:+21218434 None:intergenic
CAGAACCATTTGAAAAGCCT+TGG 0.434899 3.4:+21219606 None:intergenic
TTCTACTAAGATGACATCTA+TGG 0.437415 3.4:-21218280 MS.gene92844:CDS
GCTAAGAAGAAACCAAGAAA+TGG 0.440153 3.4:-21219972 MS.gene92844:CDS
ATCAATCTAAAATTGGTGTT+AGG 0.440240 3.4:-21219526 MS.gene92844:CDS
TCACCTACTTCTTGCTTCAC+AGG 0.457437 3.4:+21219683 None:intergenic
AAAGCCAATGGCATCTCAAA+TGG 0.469024 3.4:-21218609 MS.gene92844:CDS
GTACACTTAACTTCTCATCT+AGG 0.475567 3.4:-21218587 MS.gene92844:CDS
CGAAGAATCTCTTACAAATG+AGG 0.481362 3.4:-21219816 MS.gene92844:CDS
TCCAAGAAGCATTGCAGAGA+GGG 0.485454 3.4:-21218190 MS.gene92844:intron
AACAAGCACGGCAGATATGT+TGG 0.496382 3.4:-21217678 MS.gene92844:CDS
GGCCATCTACTAGCTTGAAA+AGG 0.500559 3.4:-21218566 MS.gene92844:CDS
AGACCTACAAGAACAAGTTC+AGG 0.503261 3.4:-21217633 MS.gene92844:intron
AGGTTTGTAAACAATGAGGT+TGG 0.522294 3.4:+21219872 None:intergenic
TGTACCATTTGAGATGCCAT+TGG 0.531059 3.4:+21218605 None:intergenic
TGATATCATCCAACAATGGA+TGG 0.538943 3.4:-21219745 MS.gene92844:CDS
GTAAAATTCGAGCGAAAAGA+GGG 0.539222 3.4:-21218224 MS.gene92844:CDS
AATGGTAATTATTCTTATGG+TGG 0.553621 3.4:-21219644 MS.gene92844:CDS
CAAAATTCATTGGTGAAGCT+AGG 0.555453 3.4:+21218444 None:intergenic
AAAATTCTGGACTACTAATG+AGG 0.563972 3.4:-21219907 MS.gene92844:CDS
AAAGGATTAAATCAGCTACA+AGG 0.569777 3.4:+21217456 None:intergenic
GTAGAAAGCTTTAAAGCCAA+TGG 0.573198 3.4:-21218621 MS.gene92844:CDS
GAATCTGTTGCACAACAGAA+TGG 0.578310 3.4:-21219662 MS.gene92844:CDS
TGATATCAACTTAGAAAACA+TGG 0.586240 3.4:+21219760 None:intergenic
GAAGAATCTCTTACAAATGA+GGG 0.589972 3.4:-21219815 MS.gene92844:CDS
GGATCTGAGTTGATTTACCA+AGG 0.594347 3.4:-21219623 MS.gene92844:CDS
GACAGAATCAAGAAACTGCA+AGG 0.604322 3.4:-21218046 MS.gene92844:CDS
GATTAGGTTTGTAAACAATG+AGG 0.615343 3.4:+21219868 None:intergenic
TGCAACATTGACATTGAGAG+AGG 0.616387 3.4:-21218643 MS.gene92844:CDS
AATTTGGCAGCAAACAAGCA+CGG 0.620596 3.4:-21217690 MS.gene92844:intron
GTCATCTTAGTAGAAGAACT+AGG 0.642089 3.4:+21218288 None:intergenic
AAACCTGTGAAGCAAGAAGT+AGG 0.642392 3.4:-21219686 MS.gene92844:CDS
AAGTTGATATCATCCAACAA+TGG 0.670047 3.4:-21219749 MS.gene92844:CDS
ATCCAAGAAGCATTGCAGAG+AGG 0.687513 3.4:-21218191 MS.gene92844:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAATATTATTATTAATTGA+AGG - chr3.4:21218790-21218809 MS.gene92844:intron 10.0%
!! AAACATACCATAATTACTAT+AGG + chr3.4:21218075-21218094 None:intergenic 20.0%
!! AACATACCATAATTACTATA+GGG + chr3.4:21218074-21218093 None:intergenic 20.0%
!! TATAGTAATTATGGTATGTT+TGG - chr3.4:21218074-21218093 MS.gene92844:intron 20.0%
!! TATGTTAATCTGAAAATTTC+AGG - chr3.4:21219370-21219389 MS.gene92844:intron 20.0%
!!! AAAATACCTTATCTGATTTT+GGG + chr3.4:21219445-21219464 None:intergenic 20.0%
!!! ATAATGTTTTCTACTTCAAA+TGG - chr3.4:21219084-21219103 MS.gene92844:intron 20.0%
!!! TAAAATACCTTATCTGATTT+TGG + chr3.4:21219446-21219465 None:intergenic 20.0%
!!! TTTAAAGTTATTACTGATGT+CGG + chr3.4:21219624-21219643 None:intergenic 20.0%
!!! TTTTTACCCTATAGTAATTA+TGG - chr3.4:21218065-21218084 MS.gene92844:CDS 20.0%
! AACTTGATTCATCATTAGTA+AGG - chr3.4:21218465-21218484 MS.gene92844:CDS 25.0%
! AATGGTAATTATTCTTATGG+TGG - chr3.4:21217815-21217834 MS.gene92844:intron 25.0%
! ACAAACCTAGTAAATGAAAA+TGG - chr3.4:21218973-21218992 MS.gene92844:intron 25.0%
! ACATAGGCTTAATAATAGTA+TGG + chr3.4:21219333-21219352 None:intergenic 25.0%
! AGAAAAAACAAATGAGCAAT+TGG - chr3.4:21218413-21218432 MS.gene92844:CDS 25.0%
! AGCTTATAAAAACAGCTTAT+AGG - chr3.4:21218219-21218238 MS.gene92844:CDS 25.0%
! ATGAAAAATATAAGCGCTAA+AGG - chr3.4:21218509-21218528 MS.gene92844:CDS 25.0%
! CAGAATGGTAATTATTCTTA+TGG - chr3.4:21217812-21217831 MS.gene92844:intron 25.0%
! GTAACTTAGTACTTCTAATT+TGG - chr3.4:21219753-21219772 MS.gene92844:CDS 25.0%
! TAATTATGGTATGTTTGGAT+TGG - chr3.4:21218079-21218098 MS.gene92844:intron 25.0%
! TCATTCAATGTGTTTATGTA+AGG + chr3.4:21219869-21219888 None:intergenic 25.0%
! TGATATCAACTTAGAAAACA+TGG + chr3.4:21217702-21217721 None:intergenic 25.0%
! TGCGTAGTTTATATACATTT+CGG - chr3.4:21219731-21219750 MS.gene92844:CDS 25.0%
!! AACACCAATTTTAGATTGAT+TGG + chr3.4:21217933-21217952 None:intergenic 25.0%
!! ATCAATCTAAAATTGGTGTT+AGG - chr3.4:21217933-21217952 MS.gene92844:intron 25.0%
!! CGCTTATATTTTTCATTTAC+AGG + chr3.4:21218505-21218524 None:intergenic 25.0%
!! GAGAATTTTCTTGAAATTCA+AGG - chr3.4:21219201-21219220 MS.gene92844:intron 25.0%
!! GAGTTTTTCTCTAATGAAAA+TGG - chr3.4:21217995-21218014 MS.gene92844:intron 25.0%
!! GATTCCAATCAATCTAAAAT+TGG - chr3.4:21217926-21217945 MS.gene92844:intron 25.0%
!! TTAGACATTGTAGATTTTGA+TGG + chr3.4:21219899-21219918 None:intergenic 25.0%
!!! ATTGCTCATTTGTTTTTTCT+TGG + chr3.4:21218413-21218432 None:intergenic 25.0%
!!! TAATATATGAAGTACTGTCA+TGG - chr3.4:21218594-21218613 MS.gene92844:CDS 25.0%
AAAATTCTGGACTACTAATG+AGG - chr3.4:21217552-21217571 MS.gene92844:intron 30.0%
AATAACTCAATATCCGAACA+GGG - chr3.4:21218319-21218338 MS.gene92844:CDS 30.0%
AGAATTGTCCCAAAATTCAT+TGG + chr3.4:21219028-21219047 None:intergenic 30.0%
ATGAAACTGTTGAATCACAT+AGG + chr3.4:21219349-21219368 None:intergenic 30.0%
CACTATCAACAACAAAATTC+TGG - chr3.4:21217539-21217558 MS.gene92844:intron 30.0%
CATTTACTAGGTTTGTAGTT+TGG + chr3.4:21218969-21218988 None:intergenic 30.0%
CCAAAATAAGTCAAACAGTT+TGG + chr3.4:21218765-21218784 None:intergenic 30.0%
CGGATATTGAGTTATTACAT+AGG + chr3.4:21218315-21218334 None:intergenic 30.0%
GAAGAATCTCTTACAAATGA+GGG - chr3.4:21217644-21217663 MS.gene92844:CDS 30.0%
GATTAGGTTTGTAAACAATG+AGG + chr3.4:21217594-21217613 None:intergenic 30.0%
TAATAACTCAATATCCGAAC+AGG - chr3.4:21218318-21218337 MS.gene92844:CDS 30.0%
TACTTGAAGAATCCATTTCT+TGG + chr3.4:21217502-21217521 None:intergenic 30.0%
TTCATCATTAGTAAGGTTTC+AGG - chr3.4:21218472-21218491 MS.gene92844:CDS 30.0%
TTCTACTAAGATGACATCTA+TGG - chr3.4:21219179-21219198 MS.gene92844:intron 30.0%
TTTGTAGTTTGGTCACAATT+TGG + chr3.4:21218958-21218977 None:intergenic 30.0%
! ACTCTTTTGCCTAATAAGAT+TGG + chr3.4:21217966-21217985 None:intergenic 30.0%
! AGCTACCATTTTCATTTACT+AGG + chr3.4:21218981-21219000 None:intergenic 30.0%
! ATGTCTTCAATCTAAAGTGT+CGG - chr3.4:21219705-21219724 MS.gene92844:CDS 30.0%
! ATTCCATTTTCAGCTATTTG+TGG + chr3.4:21218922-21218941 None:intergenic 30.0%
! ATTTACCAAGGCTTTTCAAA+TGG - chr3.4:21217848-21217867 MS.gene92844:intron 30.0%
! GTTTTCTACTTCAAATGGTT+TGG - chr3.4:21219089-21219108 MS.gene92844:intron 30.0%
! TAGCTTCACCAATGAATTTT+GGG - chr3.4:21219017-21219036 MS.gene92844:intron 30.0%
! TCTTTTCCCAAAATCAGATA+AGG - chr3.4:21219436-21219455 MS.gene92844:intron 30.0%
!! AAGTTGATATCATCCAACAA+TGG - chr3.4:21217710-21217729 MS.gene92844:intron 30.0%
!! AATGGATTTTATGGCTCTTT+TGG - chr3.4:21217878-21217897 MS.gene92844:intron 30.0%
!! CCAAACTGTTTGACTTATTT+TGG - chr3.4:21218762-21218781 MS.gene92844:intron 30.0%
!! CTTTTGAGAAAGTTAAAGTC+TGG + chr3.4:21219534-21219553 None:intergenic 30.0%
!!! GATTTTGGCTATCAAAATCT+TGG - chr3.4:21219126-21219145 MS.gene92844:intron 30.0%
!!! GCTGTTTTTATAAGCTATCT+AGG + chr3.4:21218215-21218234 None:intergenic 30.0%
!!! TTTTCAAATGGTTCTGGTAA+TGG - chr3.4:21217860-21217879 MS.gene92844:intron 30.0%
AATCTGTGAGTATCTGCAAA+AGG + chr3.4:21219936-21219955 None:intergenic 35.0%
AGAATTGTTCCATCCATTGT+TGG + chr3.4:21217726-21217745 None:intergenic 35.0%
AGGTTTGTAAACAATGAGGT+TGG + chr3.4:21217590-21217609 None:intergenic 35.0%
AGTAACGTAGTCACAGATAT+TGG - chr3.4:21219584-21219603 MS.gene92844:CDS 35.0%
ATGCCACAAATAGCTGAAAA+TGG - chr3.4:21218916-21218935 MS.gene92844:intron 35.0%
CAAAATTCATTGGTGAAGCT+AGG + chr3.4:21219018-21219037 None:intergenic 35.0%
CGAAGAATCTCTTACAAATG+AGG - chr3.4:21217643-21217662 MS.gene92844:CDS 35.0%
CTTGATTCTGTCACTAATTC+TGG + chr3.4:21219405-21219424 None:intergenic 35.0%
CTTTAGATTGAAGACATGAC+CGG + chr3.4:21219702-21219721 None:intergenic 35.0%
GCTAAGAAGAAACCAAGAAA+TGG - chr3.4:21217487-21217506 MS.gene92844:CDS 35.0%
GGAATTGCTCCAATCTTATT+AGG - chr3.4:21217954-21217973 MS.gene92844:intron 35.0%
GTAAAATTCGAGCGAAAAGA+GGG - chr3.4:21219235-21219254 MS.gene92844:intron 35.0%
GTACACTTAACTTCTCATCT+AGG - chr3.4:21218872-21218891 MS.gene92844:intron 35.0%
GTCATCTTAGTAGAAGAACT+AGG + chr3.4:21219174-21219193 None:intergenic 35.0%
TCATTAGAGAAAAACTCAGC+AGG + chr3.4:21217992-21218011 None:intergenic 35.0%
TCCATCAACAAGTTCAGAAA+TGG - chr3.4:21217673-21217692 MS.gene92844:CDS 35.0%
TGAAAACATTAAGCCCTGTT+CGG + chr3.4:21218335-21218354 None:intergenic 35.0%
TGATATCATCCAACAATGGA+TGG - chr3.4:21217714-21217733 MS.gene92844:intron 35.0%
TGTAAAATTCGAGCGAAAAG+AGG - chr3.4:21219234-21219253 MS.gene92844:intron 35.0%
TTGAATGTAAGCACATGTGT+TGG - chr3.4:21218656-21218675 MS.gene92844:intron 35.0%
! ACTATAGGGTAAAAAGCACA+CGG + chr3.4:21218060-21218079 None:intergenic 35.0%
! CTAGCTTCACCAATGAATTT+TGG - chr3.4:21219016-21219035 MS.gene92844:intron 35.0%
! CTTTTCGCTCGAATTTTACA+AGG + chr3.4:21219234-21219253 None:intergenic 35.0%
! TTTTGGTGGAGTAAACACTA+TGG - chr3.4:21217895-21217914 MS.gene92844:intron 35.0%
!! AGTTTTGTCCGTTGTATGAA+CGG - chr3.4:21218532-21218551 MS.gene92844:CDS 35.0%
!! ATCCTTTTCAAGCTAGTAGA+TGG + chr3.4:21218898-21218917 None:intergenic 35.0%
!! GTAGAAAGCTTTAAAGCCAA+TGG - chr3.4:21218838-21218857 MS.gene92844:intron 35.0%
!! TATGAAGTACTGTCATGGAT+AGG - chr3.4:21218599-21218618 MS.gene92844:CDS 35.0%
!! TCCATTTCTGAACTTGTTGA+TGG + chr3.4:21217677-21217696 None:intergenic 35.0%
!!! GGTTCTGGTAATGGATTTTA+TGG - chr3.4:21217869-21217888 MS.gene92844:intron 35.0%
AAACCTGTGAAGCAAGAAGT+AGG - chr3.4:21217773-21217792 MS.gene92844:intron 40.0%
AAAGCCAATGGCATCTCAAA+TGG - chr3.4:21218850-21218869 MS.gene92844:intron 40.0%
AAGCTATCTAGGAGAGCTTA+AGG + chr3.4:21218204-21218223 None:intergenic 40.0%
AATTTGGCAGCAAACAAGCA+CGG - chr3.4:21219769-21219788 MS.gene92844:CDS 40.0%
AGACCTACAAGAACAAGTTC+AGG - chr3.4:21219826-21219845 MS.gene92844:CDS 40.0%
CAGAACCATTTGAAAAGCCT+TGG + chr3.4:21217856-21217875 None:intergenic 40.0%
GAATCTGTTGCACAACAGAA+TGG - chr3.4:21217797-21217816 MS.gene92844:intron 40.0%
GACAGAATCAAGAAACTGCA+AGG - chr3.4:21219413-21219432 MS.gene92844:intron 40.0%
GCAATTGGACCAATGTTTGT+TGG - chr3.4:21218428-21218447 MS.gene92844:CDS 40.0%
GCAGATACTCACAGATTGTA+AGG - chr3.4:21219939-21219958 MS.gene92844:CDS 40.0%
TGCAACATTGACATTGAGAG+AGG - chr3.4:21218816-21218835 MS.gene92844:intron 40.0%
! CAAGGCTTTTCAAATGGTTC+TGG - chr3.4:21217854-21217873 MS.gene92844:intron 40.0%
! GAAGCTAGGTATGTGACTTT+TGG + chr3.4:21219004-21219023 None:intergenic 40.0%
! GACTCTCAAGAAGCAGATTT+TGG - chr3.4:21219111-21219130 MS.gene92844:intron 40.0%
! GGATTTTATGGCTCTTTTGG+TGG - chr3.4:21217881-21217900 MS.gene92844:intron 40.0%
! GTACCTGAACTTGTTCTTGT+AGG + chr3.4:21219832-21219851 None:intergenic 40.0%
! TGTACCATTTGAGATGCCAT+TGG + chr3.4:21218857-21218876 None:intergenic 40.0%
! TTTGAGCGTTGTTGTCGATT+AGG + chr3.4:21217610-21217629 None:intergenic 40.0%
!! GGATCTGAGTTGATTTACCA+AGG - chr3.4:21217836-21217855 MS.gene92844:intron 40.0%
AACAAGCACGGCAGATATGT+TGG - chr3.4:21219781-21219800 MS.gene92844:CDS 45.0%
AGACACCACTTGTCATACAC+CGG - chr3.4:21219680-21219699 MS.gene92844:CDS 45.0%
CACGGCAGATATGTTGGATT+TGG - chr3.4:21219787-21219806 MS.gene92844:CDS 45.0%
CTCCTTGGACCAACAAACAT+TGG + chr3.4:21218440-21218459 None:intergenic 45.0%
GAATCAAGTTCCTAGCTCCT+TGG + chr3.4:21218455-21218474 None:intergenic 45.0%
GACCATAGCCGTTCATACAA+CGG + chr3.4:21218543-21218562 None:intergenic 45.0%
TCACCTACTTCTTGCTTCAC+AGG + chr3.4:21217779-21217798 None:intergenic 45.0%
! GACCAATGTTTGTTGGTCCA+AGG - chr3.4:21218435-21218454 MS.gene92844:CDS 45.0%
! GGCCATCTACTAGCTTGAAA+AGG - chr3.4:21218893-21218912 MS.gene92844:intron 45.0%
!! ATCCAAGAAGCATTGCAGAG+AGG - chr3.4:21219268-21219287 MS.gene92844:intron 45.0%
!! GGCTTGTTGAGCTTATCTAC+TGG - chr3.4:21218100-21218119 MS.gene92844:intron 45.0%
!! TCCAAGAAGCATTGCAGAGA+GGG - chr3.4:21219269-21219288 MS.gene92844:intron 45.0%
ACCCTCTCTGCAATGCTTCT+TGG + chr3.4:21219273-21219292 None:intergenic 50.0%
CATGACCGGTGTATGACAAG+TGG + chr3.4:21219688-21219707 None:intergenic 50.0%
GTCCGTTGTATGAACGGCTA+TGG - chr3.4:21218538-21218557 MS.gene92844:CDS 50.0%
! GTTTGTTGGTCCAAGGAGCT+AGG - chr3.4:21218442-21218461 MS.gene92844:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 21217467 21220014 21217467 ID=MS.gene92844
chr3.4 mRNA 21217467 21220014 21217467 ID=MS.gene92844.t1;Parent=MS.gene92844
chr3.4 exon 21219465 21220014 21219465 ID=MS.gene92844.t1.exon1;Parent=MS.gene92844.t1
chr3.4 CDS 21219465 21220014 21219465 ID=cds.MS.gene92844.t1;Parent=MS.gene92844.t1
chr3.4 exon 21218191 21218669 21218191 ID=MS.gene92844.t1.exon2;Parent=MS.gene92844.t1
chr3.4 CDS 21218191 21218669 21218191 ID=cds.MS.gene92844.t1;Parent=MS.gene92844.t1
chr3.4 exon 21218024 21218089 21218024 ID=MS.gene92844.t1.exon3;Parent=MS.gene92844.t1
chr3.4 CDS 21218024 21218089 21218024 ID=cds.MS.gene92844.t1;Parent=MS.gene92844.t1
chr3.4 exon 21217634 21217702 21217634 ID=MS.gene92844.t1.exon4;Parent=MS.gene92844.t1
chr3.4 CDS 21217634 21217702 21217634 ID=cds.MS.gene92844.t1;Parent=MS.gene92844.t1
chr3.4 exon 21217467 21217538 21217467 ID=MS.gene92844.t1.exon5;Parent=MS.gene92844.t1
chr3.4 CDS 21217467 21217538 21217467 ID=cds.MS.gene92844.t1;Parent=MS.gene92844.t1
Gene Sequence

>MS.gene92844

ATGGGTGCTATGTATGAAGAGCTAAGAAGAAACCAAGAAATGGATTCTTCAAGTATTATTCATTCAAAACAACACTATCAACAACAAAATTCTGGACTACTAATGAGGTACAATTCTGCTCCAACCTCATTGTTTACAAACCTAATCGACAACAACGCTCAAAACTATATCAACAACGAAGAATCTCTTACAAATGAGGGTTATCTTCCATCAACAAGTTCAGAAATGGATACCATGTTTTCTAAGTTGATATCATCCAACAATGGATGGAACAATTCTGAAGAAAATTTGCAAGAGTTTGATGTGAAACCTGTGAAGCAAGAAGTAGGTGAATCTGTTGCACAACAGAATGGTAATTATTCTTATGGTGGATCTGAGTTGATTTACCAAGGCTTTTCAAATGGTTCTGGTAATGGATTTTATGGCTCTTTTGGTGGAGTAAACACTATGGAATCAGATGATTCCAATCAATCTAAAATTGGTGTTAGGAATTGCTCCAATCTTATTAGGCAAAAGAGTTCACCTGCTGAGTTTTTCTCTAATGAAAATGGTATGAATTTTTTCTTACTTGCATACTTTTATAGATTTTACCGTGTGCTTTTTACCCTATAGTAATTATGGTATGTTTGGATTGGCTTGTTGAGCTTATCTACTGGCATACACACTTATCAGACTATTTTGACAAGCTTATGAAAATATCTTATGCATGTTTTTAAGTTGTTTTCATCTTTTTTCCTTAAGCTCTCCTAGATAGCTTATAAAAACAGCTTATAGGAAAACAAATTTGATTTTATTCTATCTTTTATTGTAGAAATAGCTTATGCATAAGAATTTATAGATTAACTCCTATGTAATAACTCAATATCCGAACAGGGCTTAATGTTTTCATATAGTTGAAAGATAAATGTTATGATTTAAATTGAAATACTTCTCTTGTATGAAACCAAGAAAAAACAAATGAGCAATTGGACCAATGTTTGTTGGTCCAAGGAGCTAGGAACTTGATTCATCATTAGTAAGGTTTCAGGTTTGAGTCCTGTAAATGAAAAATATAAGCGCTAAAGGAGTTTTGTCCGTTGTATGAACGGCTATGGTCATCTCTGTTAAATTATGAAAATGTTCAAAATTAATATATGAAGTACTGTCATGGATAGGACACTTGACATGACAATAACACTGATGAACGTGATTGAATGTAAGCACATGTGTTGGCGTCGTGTCAGTATGAGATATTGACATCATGACATGTGTCAGTGCTACACTTTGAAAGTTACTTAGCATTAAATCAAATCAAACCAAACTGTTTGACTTATTTTGGTATTGTGAATATTATTATTAATTGAAGGTCTTGCAACATTGACATTGAGAGAGGTAGAAAGCTTTAAAGCCAATGGCATCTCAAATGGTACACTTAACTTCTCATCTAGGCCATCTACTAGCTTGAAAAGGATGCCACAAATAGCTGAAAATGGAATTCAAAGCTTAGAACCAAATTGTGACCAAACTACAAACCTAGTAAATGAAAATGGTAGCTCCAAAAGTCACATACCTAGCTTCACCAATGAATTTTGGGACAATTCTGCATTCAATGCACACAAAACAGAGAGTGAAGATGAAATAATGTTTTCTACTTCAAATGGTTTGGACTCTCAAGAAGCAGATTTTGGCTATCAAAATCTTGGTTTGACTAGTCATCTGAGTCTTCCTAGTTCTTCTACTAAGATGACATCTATGGAGAATTTTCTTGAAATTCAAGGTTCTGTTCCTTGTAAAATTCGAGCGAAAAGAGGGTTTGCAACTCATCCAAGAAGCATTGCAGAGAGGGTAAAATTTACTAACAACTATAAATCTATCATTACTCGACCATACTATTATTAAGCCTATGTGATTCAACAGTTTCATATATGTTAATCTGAAAATTTCAGGTCAGAAGAACCAGAATTAGTGACAGAATCAAGAAACTGCAAGGTCTTTTCCCAAAATCAGATAAGGTATTTTAAAGTCTGTTTATTTTCTAAACAAATTCGTAGTTTTTGTTTTTTTGAAATCGATGTTCATTTTAAACCAGACTTTAACTTTCTCAAAAGCATATCATATTGAAACTTCATATGCATTTTAGTAACGTAGTCACAGATATTGGACATGACATTGATACCGACATCAGTAATAACTTTAAAAAATGAAAGTAATTGAATGTCACATGTGTCTGTCTCAGACACCACTTGTCATACACCGGTCATGTCTTCAATCTAAAGTGTCGGTGCTGCGTAGTTTATATACATTTCGGTAACTTAGTACTTCTAATTTGGCAGCAAACAAGCACGGCAGATATGTTGGATTTGGCAGTTGAGTACATTAAAGACCTACAAGAACAAGTTCAGGTACAAGAAAATTATATACCTTACATAAACACATTGAATGATTAAATTCCATCAAAATCTACAATGTCTAACTAAATTTCTTTCACCTTTTGCAGATACTCACAGATTGTAAGGAAAAATGCAAATGTGCAAGTCATGAGAAGAAACACTGTAAACCTTGTAGCTGA
Protein sequence

>MS.gene92844.t1

MGAMYEELRRNQEMDSSSIIHSKQHYQQQNSGLLMRYNSAPTSLFTNLIDNNAQNYINNEESLTNEGYLPSTSSEMDTMFSKLISSNNGWNNSEENLQEFDVKPVKQEVGESVAQQNGNYSYGGSELIYQGFSNGSGNGFYGSFGGVNTMESDDSNQSKIGVRNCSNLIRQKSSPAEFFSNENGLATLTLREVESFKANGISNGTLNFSSRPSTSLKRMPQIAENGIQSLEPNCDQTTNLVNENGSSKSHIPSFTNEFWDNSAFNAHKTESEDEIMFSTSNGLDSQEADFGYQNLGLTSHLSLPSSSTKMTSMENFLEIQGSVPCKIRAKRGFATHPRSIAERVRRTRISDRIKKLQGLFPKSDKQTSTADMLDLAVEYIKDLQEQVQILTDCKEKCKCASHEKKHCKPCS