Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92923.t1 | XP_024642265.1 | 92.4 | 263 | 20 | 0 | 1 | 263 | 1 | 263 | 9.50E-135 | 489.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92923.t1 | A0A072TE13 | 91.6 | 263 | 22 | 0 | 1 | 263 | 1 | 263 | 8.9e-135 | 489.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene92923.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92923.t1 | MTR_1g083640 | 88.849 | 278 | 16 | 1 | 1 | 263 | 1 | 278 | 0.0 | 508 |
| MS.gene92923.t1 | MTR_0558s0020 | 91.635 | 263 | 22 | 0 | 1 | 263 | 1 | 263 | 0.0 | 506 |
| MS.gene92923.t1 | MTR_2g014100 | 85.714 | 245 | 27 | 1 | 1 | 237 | 1 | 245 | 3.01e-154 | 430 |
| MS.gene92923.t1 | MTR_8g446020 | 76.806 | 263 | 21 | 2 | 1 | 263 | 1 | 223 | 8.31e-141 | 396 |
| MS.gene92923.t1 | MTR_3g008240 | 89.655 | 203 | 21 | 0 | 1 | 203 | 1 | 203 | 1.58e-134 | 378 |
| MS.gene92923.t1 | MTR_4g040430 | 89.655 | 203 | 16 | 2 | 1 | 203 | 1 | 198 | 1.05e-131 | 371 |
| MS.gene92923.t1 | MTR_6g090505 | 89.394 | 198 | 21 | 0 | 66 | 263 | 1 | 198 | 1.13e-130 | 374 |
| MS.gene92923.t1 | MTR_0337s0040 | 71.154 | 260 | 36 | 1 | 1 | 260 | 1 | 221 | 9.76e-129 | 364 |
| MS.gene92923.t1 | MTR_5g071590 | 83.889 | 180 | 17 | 1 | 1 | 168 | 1 | 180 | 1.94e-109 | 314 |
| MS.gene92923.t1 | MTR_0019s0290 | 86.842 | 152 | 20 | 0 | 83 | 234 | 9 | 160 | 2.55e-95 | 277 |
| MS.gene92923.t1 | MTR_1g055240 | 89.510 | 143 | 15 | 0 | 91 | 233 | 2 | 144 | 3.12e-93 | 272 |
| MS.gene92923.t1 | MTR_1281s0010 | 94.495 | 109 | 6 | 0 | 20 | 128 | 1 | 109 | 9.35e-73 | 218 |
| MS.gene92923.t1 | MTR_4g008820 | 90.000 | 80 | 8 | 0 | 1 | 80 | 125 | 204 | 5.25e-41 | 149 |
| MS.gene92923.t1 | MTR_8g046060 | 84.091 | 88 | 6 | 2 | 1 | 80 | 91 | 178 | 1.17e-39 | 137 |
| MS.gene92923.t1 | MTR_6g051425 | 26.339 | 224 | 148 | 5 | 41 | 263 | 22 | 229 | 4.34e-15 | 74.3 |
| MS.gene92923.t1 | MTR_0402s0040 | 25.946 | 185 | 118 | 4 | 50 | 234 | 40 | 205 | 7.50e-15 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92923.t1 | AT4G38180 | 26.068 | 234 | 146 | 7 | 35 | 263 | 72 | 283 | 5.71e-15 | 75.1 |
Find 38 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCATGATTCGTTATTTCT+AGG | 0.256884 | 5.1:-33464115 | None:intergenic |
| TCTGATGTTGGCCTGTTGTT+AGG | 0.329765 | 5.1:-33464325 | None:intergenic |
| GAGAACGTCTCATGAAGTTA+TGG | 0.426484 | 5.1:+33464591 | MS.gene92923:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 5.1:+33464529 | MS.gene92923:CDS |
| ATGGGTATAAGATATATAGA+TGG | 0.430188 | 5.1:+33464446 | MS.gene92923:CDS |
| TTTAAGTCTGAAAACTGAAA+AGG | 0.436258 | 5.1:-33464643 | None:intergenic |
| AAGAACTATCTAATGACAAT+TGG | 0.437454 | 5.1:+33464664 | MS.gene92923:CDS |
| TCCATGATTCGTTATTTCTA+GGG | 0.439614 | 5.1:-33464114 | None:intergenic |
| TCTAATGACAATTGGTCAAA+AGG | 0.441108 | 5.1:+33464672 | MS.gene92923:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 5.1:+33464394 | MS.gene92923:CDS |
| GCTCTAATAAATTCTAAAGC+TGG | 0.462891 | 5.1:+33464559 | MS.gene92923:CDS |
| AGAACGTCTCATGAAGTTAT+GGG | 0.466325 | 5.1:+33464592 | MS.gene92923:CDS |
| CCCCTAGAAATAACGAATCA+TGG | 0.469857 | 5.1:+33464113 | MS.gene92923:CDS |
| GTTTGCTTAATCCTAACAAC+AGG | 0.484661 | 5.1:+33464314 | MS.gene92923:CDS |
| TTACTTCTCGAAATGATCTC+AGG | 0.488412 | 5.1:-33464516 | None:intergenic |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 5.1:+33464277 | MS.gene92923:CDS |
| CTCAGGTAACACAAATGCTC+TGG | 0.496027 | 5.1:-33464499 | None:intergenic |
| AGCTGGAATATCTTTGAAGC+TGG | 0.496270 | 5.1:+33464411 | MS.gene92923:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.502941 | 5.1:+33464604 | MS.gene92923:CDS |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 5.1:-33464388 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 5.1:+33464288 | MS.gene92923:CDS |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 5.1:+33464181 | MS.gene92923:CDS |
| TGGGTATAAGATATATAGAT+GGG | 0.523615 | 5.1:+33464447 | MS.gene92923:CDS |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 5.1:+33464243 | MS.gene92923:CDS |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 5.1:+33464289 | MS.gene92923:CDS |
| CCATGATTCGTTATTTCTAG+GGG | 0.558064 | 5.1:-33464113 | None:intergenic |
| TGTCTTCCGCAGTATCAGCT+TGG | 0.558294 | 5.1:-33464777 | None:intergenic |
| GACAATTGGTCAAAAGGAGA+TGG | 0.566160 | 5.1:+33464678 | MS.gene92923:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.566406 | 5.1:+33464453 | MS.gene92923:CDS |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 5.1:+33464607 | MS.gene92923:CDS |
| TCTCATGAAGTTATGGGTCA+AGG | 0.593601 | 5.1:+33464598 | MS.gene92923:CDS |
| GGTCAAAAGGAGATGGTGGT+TGG | 0.599651 | 5.1:+33464685 | MS.gene92923:CDS |
| ACCAGTGGATTGTCTGATGT+TGG | 0.601070 | 5.1:-33464337 | None:intergenic |
| ATATCAACTTCATTTGAGAG+TGG | 0.601371 | 5.1:+33464034 | MS.gene92923:CDS |
| TTCACAAACTCAATAACCAG+TGG | 0.611860 | 5.1:-33464352 | None:intergenic |
| GCCAACATCAGACAATCCAC+TGG | 0.642242 | 5.1:+33464336 | MS.gene92923:CDS |
| TGACATCCAAGCTGATACTG+CGG | 0.664160 | 5.1:+33464771 | MS.gene92923:CDS |
| AATTGGTCAAAAGGAGATGG+TGG | 0.680841 | 5.1:+33464681 | MS.gene92923:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATCAGATA+AGG | + | chr5.1:33464243-33464262 | MS.gene92923:CDS | 20.0% |
| !!! | AAAGAGATTGAAGATTTTTA+TGG | + | chr5.1:33464160-33464179 | MS.gene92923:CDS | 20.0% |
| ! | AAGAACTATCTAATGACAAT+TGG | + | chr5.1:33464664-33464683 | MS.gene92923:CDS | 25.0% |
| ! | ATGGGTATAAGATATATAGA+TGG | + | chr5.1:33464446-33464465 | MS.gene92923:CDS | 25.0% |
| ! | TGGGTATAAGATATATAGAT+GGG | + | chr5.1:33464447-33464466 | MS.gene92923:CDS | 25.0% |
| ! | TTTAAGTCTGAAAACTGAAA+AGG | - | chr5.1:33464646-33464665 | None:intergenic | 25.0% |
| !! | ATAACACTGGATTTTCTATT+AGG | + | chr5.1:33464194-33464213 | MS.gene92923:CDS | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | + | chr5.1:33464289-33464308 | MS.gene92923:CDS | 30.0% | |
| ATATCAACTTCATTTGAGAG+TGG | + | chr5.1:33464034-33464053 | MS.gene92923:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | + | chr5.1:33464277-33464296 | MS.gene92923:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | + | chr5.1:33464181-33464200 | MS.gene92923:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | + | chr5.1:33464288-33464307 | MS.gene92923:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | + | chr5.1:33464453-33464472 | MS.gene92923:CDS | 30.0% | |
| TCTAATGACAATTGGTCAAA+AGG | + | chr5.1:33464672-33464691 | MS.gene92923:CDS | 30.0% | |
| TGGATGTCATAGTAAAAAGA+TGG | - | chr5.1:33464760-33464779 | None:intergenic | 30.0% | |
| !! | GCTCTAATAAATTCTAAAGC+TGG | + | chr5.1:33464559-33464578 | MS.gene92923:CDS | 30.0% |
| !! | TCCATGATTCGTTATTTCTA+GGG | - | chr5.1:33464117-33464136 | None:intergenic | 30.0% |
| !! | TTCCATGATTCGTTATTTCT+AGG | - | chr5.1:33464118-33464137 | None:intergenic | 30.0% |
| AGAACGTCTCATGAAGTTAT+GGG | + | chr5.1:33464592-33464611 | MS.gene92923:CDS | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | + | chr5.1:33464529-33464548 | MS.gene92923:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | + | chr5.1:33464378-33464397 | MS.gene92923:CDS | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | + | chr5.1:33464372-33464391 | MS.gene92923:CDS | 35.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | + | chr5.1:33464314-33464333 | MS.gene92923:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | + | chr5.1:33464377-33464396 | MS.gene92923:CDS | 35.0% | |
| TTACTTCTCGAAATGATCTC+AGG | - | chr5.1:33464519-33464538 | None:intergenic | 35.0% | |
| TTCACAAACTCAATAACCAG+TGG | - | chr5.1:33464355-33464374 | None:intergenic | 35.0% | |
| !! | CCATGATTCGTTATTTCTAG+GGG | - | chr5.1:33464116-33464135 | None:intergenic | 35.0% |
| !!! | AAGCTGGATGAAGTTTTGAA+TGG | + | chr5.1:33464427-33464446 | MS.gene92923:CDS | 35.0% |
| !!! | AGCTGGATGAAGTTTTGAAT+GGG | + | chr5.1:33464428-33464447 | MS.gene92923:CDS | 35.0% |
| AATTGGTCAAAAGGAGATGG+TGG | + | chr5.1:33464681-33464700 | MS.gene92923:CDS | 40.0% | |
| CCCCTAGAAATAACGAATCA+TGG | + | chr5.1:33464113-33464132 | MS.gene92923:CDS | 40.0% | |
| GACAATTGGTCAAAAGGAGA+TGG | + | chr5.1:33464678-33464697 | MS.gene92923:CDS | 40.0% | |
| GAGAACGTCTCATGAAGTTA+TGG | + | chr5.1:33464591-33464610 | MS.gene92923:CDS | 40.0% | |
| TCTCATGAAGTTATGGGTCA+AGG | + | chr5.1:33464598-33464617 | MS.gene92923:CDS | 40.0% | |
| !! | AGCTGGAATATCTTTGAAGC+TGG | + | chr5.1:33464411-33464430 | MS.gene92923:CDS | 40.0% |
| CTCAGGTAACACAAATGCTC+TGG | - | chr5.1:33464502-33464521 | None:intergenic | 45.0% | |
| GAAAAACCTGAGGAAAGGGT+TGG | + | chr5.1:33464382-33464401 | MS.gene92923:CDS | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | + | chr5.1:33464604-33464623 | MS.gene92923:CDS | 45.0% | |
| TGACATCCAAGCTGATACTG+CGG | + | chr5.1:33464771-33464790 | MS.gene92923:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | - | chr5.1:33464391-33464410 | None:intergenic | 45.0% | |
| ! | TCTGATGTTGGCCTGTTGTT+AGG | - | chr5.1:33464328-33464347 | None:intergenic | 45.0% |
| !! | ACCAGTGGATTGTCTGATGT+TGG | - | chr5.1:33464340-33464359 | None:intergenic | 45.0% |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | + | chr5.1:33464394-33464413 | MS.gene92923:CDS | 45.0% |
| GCCAACATCAGACAATCCAC+TGG | + | chr5.1:33464336-33464355 | MS.gene92923:CDS | 50.0% | |
| GTTATGGGTCAAGGAGTTGG+AGG | + | chr5.1:33464607-33464626 | MS.gene92923:CDS | 50.0% | |
| TGTCTTCCGCAGTATCAGCT+TGG | - | chr5.1:33464780-33464799 | None:intergenic | 50.0% | |
| ! | GGTCAAAAGGAGATGGTGGT+TGG | + | chr5.1:33464685-33464704 | MS.gene92923:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 33464025 | 33464834 | 33464025 | ID=MS.gene92923 |
| chr5.1 | mRNA | 33464025 | 33464834 | 33464025 | ID=MS.gene92923.t1;Parent=MS.gene92923 |
| chr5.1 | exon | 33464025 | 33464834 | 33464025 | ID=MS.gene92923.t1.exon1;Parent=MS.gene92923.t1 |
| chr5.1 | CDS | 33464025 | 33464834 | 33464025 | ID=cds.MS.gene92923.t1;Parent=MS.gene92923.t1 |
| Gene Sequence |
| Protein sequence |
