AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93022

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93022.t1 MTR_8g071030 86.805 773 70 3 1 765 1 749 0.0 1320
MS.gene93022.t1 MTR_8g070070 74.648 781 170 8 1 765 1 769 0.0 1151
MS.gene93022.t1 MTR_8g070075 65.096 785 220 9 1 761 4 758 0.0 1018
MS.gene93022.t1 MTR_1g035420 63.197 538 183 6 229 764 134 658 0.0 698
MS.gene93022.t1 MTR_1g035400 61.702 470 177 3 288 756 101 568 0.0 608
MS.gene93022.t1 MTR_6g044690 57.204 465 176 8 298 753 114 564 0.0 536
MS.gene93022.t1 MTR_0376s0030 50.980 510 223 8 267 764 83 577 2.60e-171 505
MS.gene93022.t1 MTR_6g043960 51.293 464 184 8 298 753 53 482 9.10e-157 464
MS.gene93022.t1 MTR_2g054460 43.937 569 202 13 194 753 4 464 2.84e-143 429
MS.gene93022.t1 MTR_7g088370 43.100 471 226 17 296 753 239 680 6.62e-107 342
MS.gene93022.t1 MTR_5g013420 40.000 460 253 8 296 751 618 1058 1.09e-105 348
MS.gene93022.t1 MTR_5g013420 40.000 460 253 8 296 751 618 1058 1.20e-105 348
MS.gene93022.t1 MTR_3g011440 39.270 466 265 9 296 752 177 633 5.83e-105 336
MS.gene93022.t1 MTR_4g010830 40.741 459 250 9 296 751 510 949 2.65e-102 337
MS.gene93022.t1 MTR_8g042750 40.169 473 250 13 296 753 204 658 2.19e-101 327
MS.gene93022.t1 MTR_4g010550 38.745 462 225 12 296 751 24 433 2.64e-93 299
MS.gene93022.t1 MTR_7g084090 37.759 482 260 14 296 752 261 727 2.98e-87 291
MS.gene93022.t1 MTR_6g061270 37.630 481 261 13 296 752 199 664 7.49e-86 286
MS.gene93022.t1 MTR_6g061200 37.209 473 245 15 296 752 199 635 5.13e-80 270
MS.gene93022.t1 MTR_7g084090 41.047 363 184 11 296 641 261 610 2.39e-71 246
MS.gene93022.t1 MTR_7g445710 31.667 480 229 17 295 751 109 512 1.14e-53 194
MS.gene93022.t1 MTR_7g445530 31.458 480 230 17 295 751 109 512 1.22e-53 194
MS.gene93022.t1 MTR_1g048950 32.766 470 265 15 298 751 73 507 1.15e-51 189
MS.gene93022.t1 MTR_7g450730 30.131 458 228 15 296 751 107 474 1.28e-50 185
MS.gene93022.t1 MTR_7g052480 31.613 310 186 9 459 752 227 526 1.16e-38 152
MS.gene93022.t1 MTR_7g445600 34.000 150 62 2 602 751 243 355 2.89e-22 99.8
MS.gene93022.t1 MTR_7g445600 37.984 129 66 5 296 424 106 220 4.32e-15 78.2
MS.gene93022.t1 MTR_6g044000 67.925 53 17 0 701 753 117 169 1.13e-20 90.5
MS.gene93022.t1 MTR_6g044000 51.471 68 33 0 298 365 53 120 7.63e-18 82.0
MS.gene93022.t1 MTR_8g078505 27.857 280 155 8 503 751 252 515 2.14e-20 95.9
MS.gene93022.t1 MTR_5g018850 31.492 181 112 3 568 747 1310 1479 4.19e-20 96.7
MS.gene93022.t1 MTR_8g078490 29.730 185 118 3 568 751 1056 1229 4.89e-20 96.3
MS.gene93022.t1 MTR_4g132610 29.101 189 112 3 569 751 851 1023 9.14e-19 92.0
MS.gene93022.t1 MTR_5g098680 36.076 158 87 4 321 473 267 415 4.26e-16 82.8
MS.gene93022.t1 MTR_8g102580 36.076 158 87 4 321 473 261 409 1.00e-15 81.6
MS.gene93022.t1 MTR_7g445810 30.882 204 100 8 270 450 79 264 9.35e-13 69.7
MS.gene93022.t1 MTR_3g114910 33.333 153 88 4 321 473 187 325 1.33e-12 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93022.t1 AT4G13460 61.443 485 180 5 282 764 170 649 0.0 632
MS.gene93022.t1 AT4G13460 61.443 485 180 5 282 764 170 649 0.0 632
MS.gene93022.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 601
MS.gene93022.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 601
MS.gene93022.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 601
MS.gene93022.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 601
MS.gene93022.t1 AT5G04940 40.373 483 258 15 279 753 180 640 1.17e-98 320
MS.gene93022.t1 AT5G04940 40.373 483 258 15 279 753 180 640 1.17e-98 320
MS.gene93022.t1 AT1G73100 36.347 553 312 15 220 753 108 639 3.81e-96 313
MS.gene93022.t1 AT2G22740 36.611 478 276 8 285 751 298 759 3.30e-93 308
MS.gene93022.t1 AT2G22740 36.611 478 276 8 285 751 298 759 3.30e-93 308
MS.gene93022.t1 AT2G35160 35.048 525 290 13 236 751 245 727 2.16e-90 300
MS.gene93022.t1 AT2G35160 35.048 525 290 13 236 751 281 763 4.24e-90 300
MS.gene93022.t1 AT2G35160 35.048 525 290 13 236 751 281 763 4.24e-90 300
MS.gene93022.t1 AT2G35160 35.048 525 290 13 236 751 281 763 4.24e-90 300
MS.gene93022.t1 AT2G35160 35.048 525 290 13 236 751 281 763 4.24e-90 300
MS.gene93022.t1 AT5G13960 37.527 469 245 16 313 752 145 594 1.47e-83 278
MS.gene93022.t1 AT5G13960 37.527 469 245 16 313 752 11 460 2.89e-83 274
MS.gene93022.t1 AT1G17770 32.031 512 313 16 258 753 169 661 9.03e-73 251
MS.gene93022.t1 AT2G24740 38.117 446 242 14 314 753 307 724 7.14e-72 250
MS.gene93022.t1 AT5G47150 39.888 178 98 4 296 473 156 324 2.33e-27 114
MS.gene93022.t1 AT5G47160 39.766 171 93 5 300 469 246 407 8.36e-24 105
MS.gene93022.t1 AT5G47160 39.766 171 93 5 300 469 246 407 8.36e-24 105
MS.gene93022.t1 AT5G47160 39.766 171 93 5 300 469 246 407 8.36e-24 105
MS.gene93022.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 6.34e-22 102
MS.gene93022.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 6.34e-22 102
MS.gene93022.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 6.34e-22 102
MS.gene93022.t1 AT2G23740 30.964 197 109 5 570 756 1177 1356 6.55e-22 102
MS.gene93022.t1 AT2G05900 38.506 174 99 6 358 531 26 191 1.21e-19 90.9
MS.gene93022.t1 AT1G57820 36.047 172 86 6 317 473 264 426 1.10e-16 84.7
MS.gene93022.t1 AT1G57820 36.047 172 86 6 317 473 264 426 1.10e-16 84.7
MS.gene93022.t1 AT3G03750 30.918 207 115 6 548 752 143 323 3.55e-16 81.3
MS.gene93022.t1 AT1G57820 35.503 169 88 6 317 473 264 423 1.47e-15 81.3
MS.gene93022.t1 AT5G39550 34.302 172 89 6 317 473 249 411 1.53e-15 81.3
MS.gene93022.t1 AT2G23740 30.405 148 79 4 570 710 1184 1314 6.39e-15 79.7
MS.gene93022.t1 AT2G23740 30.405 148 79 4 570 710 1184 1314 6.39e-15 79.7
MS.gene93022.t1 AT1G66040 31.977 172 93 5 317 473 249 411 9.29e-15 78.6
MS.gene93022.t1 AT1G66050 31.977 172 93 5 317 473 249 411 9.71e-15 78.6

Find 230 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAATCTCAACCTTAATT+TGG 0.115237 8.2:-32234475 MS.gene93022:CDS
GCTAGGGATATTAAGAAAAT+TGG 0.127502 8.2:-32233439 MS.gene93022:CDS
CCAAATTAAGGTTGAGATTT+AGG 0.131281 8.2:+32234475 None:intergenic
GCTACTAGTGTAATTGTTTC+AGG 0.143034 8.2:-32233805 MS.gene93022:CDS
CCCCATTCTACCCATCTATT+TGG 0.166114 8.2:+32232896 None:intergenic
CTATTGATATTTCAAATAAT+AGG 0.206413 8.2:-32233387 MS.gene93022:CDS
GCAAATTTGTGATCAGAAAT+TGG 0.219259 8.2:-32233698 MS.gene93022:CDS
ACATACTGAATGAATATATT+TGG 0.228005 8.2:+32232749 None:intergenic
GGTGGATATGGTGGTGATTT+GGG 0.244564 8.2:+32234734 None:intergenic
TAGGTCCTTGGACCTTATTC+AGG 0.261644 8.2:-32233020 MS.gene93022:CDS
AAGCAATCCATGTAGATTAT+AGG 0.265044 8.2:+32233177 None:intergenic
CTAAATCTCAACCTTAATTT+GGG 0.267648 8.2:-32234474 MS.gene93022:CDS
GGTGAGAGCGGTGTGATTTA+AGG 0.267982 8.2:+32234761 None:intergenic
CGGTGGATATGGTGGTGATT+TGG 0.269392 8.2:+32234733 None:intergenic
TCAGATTCGGGAGAGAAATT+AGG 0.279456 8.2:+32234630 None:intergenic
TGATTTAAGGTTGAAATTCT+AGG 0.282052 8.2:+32234774 None:intergenic
TAGGTTGTTGTTGGGAATCT+TGG 0.291847 8.2:+32234649 None:intergenic
AATTGGTTGTTGAAGTTGAT+AGG 0.309699 8.2:-32234209 MS.gene93022:CDS
TATTGATATTTCAAATAATA+GGG 0.311494 8.2:-32233386 MS.gene93022:CDS
TGATCATCGGTTTCAGATTC+GGG 0.313526 8.2:+32234618 None:intergenic
TGAAAGTGGGTGAGGAGAAT+AGG 0.314740 8.2:-32234101 MS.gene93022:CDS
GCAACACGGTTTCGACAATT+AGG 0.314745 8.2:+32233103 None:intergenic
GATATAGGAAAGTCTGGTTT+TGG 0.315495 8.2:-32233529 MS.gene93022:CDS
GAATGGGTTTCAAGGGTTAA+TGG 0.316851 8.2:+32234876 None:intergenic
TTGATCATCGGTTTCAGATT+CGG 0.324605 8.2:+32234617 None:intergenic
AATATAGCTTGGAAGTGTTT+AGG 0.327076 8.2:-32233066 MS.gene93022:CDS
AATGAATGAGGAGAAGAAAT+TGG 0.327732 8.2:-32234226 MS.gene93022:CDS
GACGCGAAGAAATTGTTAAA+AGG 0.332988 8.2:+32234672 None:intergenic
ATATATTTGGACTCGAACTA+TGG 0.334818 8.2:+32232762 None:intergenic
ATCTTGCTCGCCCACTCGTC+CGG 0.340319 8.2:+32232629 None:intergenic
TTAGGAGGACATGAGCAAAA+AGG 0.343616 8.2:+32233121 None:intergenic
AGAGAACTCTGCATAGATTA+TGG 0.345054 8.2:-32232656 MS.gene93022:CDS
GAAAGTGGGTGAGGAGAATA+GGG 0.346272 8.2:-32234100 MS.gene93022:CDS
AATGGGTTTCAAGGGTTAAT+GGG 0.348241 8.2:+32234877 None:intergenic
GGGAGACAATTAATGGAATA+AGG 0.348461 8.2:-32234056 MS.gene93022:CDS
AGCAATCCATGTAGATTATA+GGG 0.350043 8.2:+32233178 None:intergenic
ATATCATTAAATAGCCTAAC+TGG 0.350719 8.2:+32233355 None:intergenic
GTTAGTTGCTTGTTTGATAT+AGG 0.351027 8.2:-32233544 MS.gene93022:CDS
AGAAATTGTTAAAAGGTTCC+AGG 0.355471 8.2:+32234679 None:intergenic
CACAGACAGGTTGGGGAGTT+AGG 0.355710 8.2:-32233039 MS.gene93022:CDS
ATTGGATGAGGAGAAGATAT+TGG 0.356889 8.2:-32234154 MS.gene93022:CDS
CTAGTACATTTCCAAATAGA+TGG 0.359463 8.2:-32232907 MS.gene93022:CDS
GAAGGGAGTGCATCTACTTC+TGG 0.362995 8.2:-32233601 MS.gene93022:CDS
TAAAGGATCCAGCCTGAATA+AGG 0.368631 8.2:+32233008 None:intergenic
ATTGTCTGAGGAAAGGAAAT+TGG 0.371576 8.2:-32234172 MS.gene93022:CDS
TGTGGTAGAATTTGGTGTAT+AGG 0.373147 8.2:+32234589 None:intergenic
AAGTGATGTTCCGGTGGATA+TGG 0.376573 8.2:+32234722 None:intergenic
GTCGAGTTTGATGGCAGATA+TGG 0.380874 8.2:-32234391 MS.gene93022:CDS
TAGAATTTGGTGTATAGGTT+GGG 0.381830 8.2:+32234594 None:intergenic
ACCAGTATACTCGCAGATAA+AGG 0.385474 8.2:+32232991 None:intergenic
TATGGTGGTGATTTGGGGTG+GGG 0.386266 8.2:+32234740 None:intergenic
TTGTTAAAAGGTTCCAGGTT+TGG 0.388510 8.2:+32234684 None:intergenic
CGTAGTATCAACCACATTCA+TGG 0.388913 8.2:-32234030 MS.gene93022:CDS
CTAGGGATATTAAGAAAATT+GGG 0.394850 8.2:-32233438 MS.gene93022:CDS
TCGAAACCGTGTTGCACAAA+AGG 0.395320 8.2:-32233095 MS.gene93022:CDS
TGTTGTTTCATTGGAGAATA+CGG 0.399431 8.2:-32234517 MS.gene93022:CDS
TCCAAATAGATGGGTAGAAT+GGG 0.401426 8.2:-32232897 MS.gene93022:CDS
GAGAGAAATTAGGTTGTTGT+TGG 0.401584 8.2:+32234640 None:intergenic
TGCACTATGGGATTGAGGTT+AGG 0.405470 8.2:-32233645 MS.gene93022:CDS
ACCACATTCATGGTTATATG+AGG 0.405787 8.2:-32234020 MS.gene93022:CDS
TCAAAGAGTATGTCTTATAA+TGG 0.405858 8.2:-32233838 MS.gene93022:CDS
GTAGAATTTGGTGTATAGGT+TGG 0.407762 8.2:+32234593 None:intergenic
GATATGGTGGTGATTTGGGG+TGG 0.408728 8.2:+32234738 None:intergenic
AGAAATAGAAGTAGAAGAAT+GGG 0.413089 8.2:+32234860 None:intergenic
GCACTATGGGATTGAGGTTA+GGG 0.416487 8.2:-32233644 MS.gene93022:CDS
TTCCAAATAGATGGGTAGAA+TGG 0.421060 8.2:-32232898 MS.gene93022:CDS
TTGTTTGATATAGGAAAGTC+TGG 0.421956 8.2:-32233535 MS.gene93022:CDS
TAGGTCTACACAGACAGGTT+GGG 0.422135 8.2:-32233047 MS.gene93022:CDS
GAGAAATAGAAGTAGAAGAA+TGG 0.422660 8.2:+32234859 None:intergenic
TGAAACAACAAAATCATTGT+TGG 0.424236 8.2:+32234530 None:intergenic
TTTGATGGCAGATATGGTTA+TGG 0.424584 8.2:-32234385 MS.gene93022:CDS
TCCTTTATCTGCGAGTATAC+TGG 0.426481 8.2:-32232992 MS.gene93022:CDS
AGGTTGTTGTTGGGAATCTT+GGG 0.427326 8.2:+32234650 None:intergenic
ATATGGTGGTGATTTGGGGT+GGG 0.427929 8.2:+32234739 None:intergenic
CATCTATTTGGAAATGTACT+AGG 0.428903 8.2:+32232908 None:intergenic
GAAACAAGAGTCGAGTTTGA+TGG 0.434269 8.2:-32234400 MS.gene93022:CDS
AAGTAGAAGAATGGGTTTCA+AGG 0.435832 8.2:+32234868 None:intergenic
TATAAGTTATTGAGAATTGA+GGG 0.436671 8.2:-32233496 MS.gene93022:CDS
GGCAGAGATGAAAGTGGGTG+AGG 0.437445 8.2:-32234109 MS.gene93022:CDS
TCTTTATTATGAGTATCTTC+CGG 0.438277 8.2:-32233314 MS.gene93022:CDS
ATTGGTTGTTGAAGTTGATA+GGG 0.446123 8.2:-32234208 MS.gene93022:CDS
TCCTTGGACCTTATTCAGGC+TGG 0.446886 8.2:-32233016 MS.gene93022:CDS
CGAAACCGTGTTGCACAAAA+GGG 0.448108 8.2:-32233094 MS.gene93022:CDS
TAATGAAGGAGCGTGGACTA+TGG 0.448333 8.2:-32233987 MS.gene93022:CDS
AATTGAGGGGCAGGCGAAGA+TGG 0.448823 8.2:-32233482 MS.gene93022:CDS
AAACTCGACTCTTGTTTCTC+AGG 0.449003 8.2:+32234405 None:intergenic
AGGAGGGAATTGTGTGTGAC+TGG 0.450008 8.2:-32233898 MS.gene93022:CDS
ACCTAGTGGTTGTGAATGTA+AGG 0.454638 8.2:-32233251 MS.gene93022:CDS
GGAATAGGAACATGAGAAGA+AGG 0.457689 8.2:+32234834 None:intergenic
AGAGAAATTAGGTTGTTGTT+GGG 0.464968 8.2:+32234641 None:intergenic
TTGATGAGAAATACTAAGAT+CGG 0.467844 8.2:+32234800 None:intergenic
TGGCGTTTGAAGTGATGTTC+CGG 0.469579 8.2:+32234713 None:intergenic
GATTTAAGGTTGAAATTCTA+GGG 0.470586 8.2:+32234775 None:intergenic
TTGGAAAGGAGTATGCACTA+TGG 0.475190 8.2:-32233658 MS.gene93022:CDS
ACATCACCAACATAAATTCC+AGG 0.479414 8.2:+32233931 None:intergenic
TAAGGTTGAGATTTAGGTCC+GGG 0.480083 8.2:+32234481 None:intergenic
TGATGAGAAATACTAAGATC+GGG 0.480139 8.2:+32234801 None:intergenic
GGTTTGTGTTTCATCAGAGT+GGG 0.481554 8.2:-32233276 MS.gene93022:CDS
AGTAGAAGAATGGGTTTCAA+GGG 0.482906 8.2:+32234869 None:intergenic
GGATATTAAGAAAATTGGGT+TGG 0.483689 8.2:-32233434 MS.gene93022:CDS
AGGATAGTTGGACCGATACC+TGG 0.485997 8.2:-32233949 MS.gene93022:CDS
TTCGCTTCGCGTGAGAATTG+AGG 0.488587 8.2:-32234259 MS.gene93022:CDS
TCTTGCTCGCCCACTCGTCC+GGG 0.490402 8.2:+32232630 None:intergenic
GGTTATGGAGAAGGAAAGCC+AGG 0.494082 8.2:-32234370 MS.gene93022:CDS
AATTGTTTCAGGTGGTTATG+AGG 0.495448 8.2:-32233794 MS.gene93022:CDS
TAATCTAAAGGAGGTATTGA+CGG 0.498715 8.2:+32232830 None:intergenic
ATAGGGAAAATGTTCCAGTT+AGG 0.499113 8.2:-32233369 MS.gene93022:CDS
TATCGTGATCAAAGGATAGT+TGG 0.501111 8.2:-32233961 MS.gene93022:CDS
GTATAAGTTATTGAGAATTG+AGG 0.501728 8.2:-32233497 MS.gene93022:CDS
CACTGTTCTCAAAGATGCTA+GGG 0.502270 8.2:-32233455 MS.gene93022:CDS
CCCTCACAAGTTCCATCACC+TGG 0.503770 8.2:+32234352 None:intergenic
TCATGTCATGTTATATGCAA+TGG 0.506033 8.2:-32232696 MS.gene93022:CDS
TCTATGCAGAGTTCTCTCAT+AGG 0.506224 8.2:+32232662 None:intergenic
GTCGGCTACCAACGTGGAGG+GGG 0.510887 8.2:-32234076 MS.gene93022:CDS
AAAGGGGCTCAAATATAGCT+TGG 0.511664 8.2:-32233077 MS.gene93022:CDS
GAATCAGCGATTTCGAGAAA+AGG 0.512004 8.2:+32234552 None:intergenic
AGGTCTACACAGACAGGTTG+GGG 0.513098 8.2:-32233046 MS.gene93022:CDS
GTATAGGTTGGGTTGATCAT+CGG 0.513117 8.2:+32234605 None:intergenic
GATTTGGGGTGGGGTGAGAG+CGG 0.514925 8.2:+32234749 None:intergenic
GGGGTGGATTCTGTAAATGA+TGG 0.515279 8.2:-32234453 MS.gene93022:CDS
ACTCGAACTATGGCTCATAT+AGG 0.515301 8.2:+32232772 None:intergenic
TTAAGGTTGAGATTTAGGTC+CGG 0.515596 8.2:+32234480 None:intergenic
GAGTGCATCTACTTCTGGTA+AGG 0.518621 8.2:-32233596 MS.gene93022:CDS
TCAATAGAGATACAACTCAT+TGG 0.521177 8.2:+32233403 None:intergenic
TGCACTTTCGTCATAAGATA+AGG 0.521451 8.2:-32234302 MS.gene93022:CDS
GTGTCGGCTACCAACGTGGA+GGG 0.521469 8.2:-32234078 MS.gene93022:CDS
GAGTTTCCCTATAATCTACA+TGG 0.522663 8.2:-32233184 MS.gene93022:CDS
GCACTGTTCTCAAAGATGCT+AGG 0.522783 8.2:-32233456 MS.gene93022:CDS
TGTCGGCTACCAACGTGGAG+GGG 0.522877 8.2:-32234077 MS.gene93022:CDS
GCATACTCCTTTCCAATGCA+AGG 0.525232 8.2:+32233665 None:intergenic
GGAGGTATTGACGGATAAGA+TGG 0.528649 8.2:+32232839 None:intergenic
AATCAGCGATTTCGAGAAAA+GGG 0.530354 8.2:+32234553 None:intergenic
AGAAGAGATTGTCTGAGGAA+AGG 0.532258 8.2:-32234179 MS.gene93022:CDS
GGAGAAGGAAAGCCAGGTGA+TGG 0.532924 8.2:-32234364 MS.gene93022:CDS
TGGTTTGTGTTTCATCAGAG+TGG 0.533225 8.2:-32233277 MS.gene93022:CDS
ATTATAATCTACACTGGCCA+TGG 0.534081 8.2:-32233745 MS.gene93022:CDS
GGCAGATATGGTTATGGAGA+AGG 0.536146 8.2:-32234379 MS.gene93022:CDS
AACAATGAATGGGGTCTCGT+TGG 0.538408 8.2:-32232936 MS.gene93022:CDS
GTTATTGAGAATTGAGGGGC+AGG 0.539794 8.2:-32233491 MS.gene93022:CDS
ACAATTACACTAGTAGCAAT+CGG 0.540891 8.2:+32233811 None:intergenic
TGGAAAGGAGTATGCACTAT+GGG 0.541585 8.2:-32233657 MS.gene93022:CDS
TACTGGTGTTGCTATGACTA+GGG 0.544129 8.2:-32232975 MS.gene93022:CDS
AATTCTTCTCTCGCCCTCCA+TGG 0.544307 8.2:+32233728 None:intergenic
GGTTGTTGAAGTTGATAGGG+AGG 0.545333 8.2:-32234205 MS.gene93022:CDS
ACATCCAGAAAATAATCTAA+AGG 0.546485 8.2:+32232818 None:intergenic
CTGATCACAAATTTGCCTTG+AGG 0.547153 8.2:+32233705 None:intergenic
ATTGAGGGGCAGGCGAAGAT+GGG 0.548419 8.2:-32233481 MS.gene93022:CDS
AACGCCACTCATTCCAAACC+TGG 0.552250 8.2:-32234697 MS.gene93022:CDS
GAGTATGCACTATGGGATTG+AGG 0.552471 8.2:-32233650 MS.gene93022:CDS
CCAGGTGATGGAACTTGTGA+GGG 0.555665 8.2:-32234352 MS.gene93022:CDS
ATGCAGAGTTCTCTCATAGG+AGG 0.558353 8.2:+32232665 None:intergenic
GGAGGGTGGAAACCTTGCAT+TGG 0.558530 8.2:-32233677 MS.gene93022:CDS
CTCTGATGAAACACAAACCA+CGG 0.558774 8.2:+32233280 None:intergenic
GGAAGATACTCATAATAAAG+AGG 0.560347 8.2:+32233316 None:intergenic
GGTAAGGTTTATGTTTACGA+TGG 0.560574 8.2:-32233580 MS.gene93022:CDS
GTTAATGGGAAAGGATCCAT+TGG 0.561622 8.2:+32234891 None:intergenic
GAGGGCTGCGTCATTAATGA+AGG 0.566875 8.2:-32234001 MS.gene93022:CDS
TGAGGAAAGGAAATTGGATG+AGG 0.567529 8.2:-32234166 MS.gene93022:CDS
ATTCGCGGGATGAAGTATGA+AGG 0.568414 8.2:-32233619 MS.gene93022:CDS
GGAGGACAAGAGAATGAATG+AGG 0.571585 8.2:-32234238 MS.gene93022:CDS
GGAAGAGAAGAGATTGTCTG+AGG 0.572868 8.2:-32234184 MS.gene93022:CDS
AACCACGGGAAAGTAGTATC+CGG 0.574690 8.2:+32233295 None:intergenic
CCGATACCTGGAATTTATGT+TGG 0.577931 8.2:-32233937 MS.gene93022:CDS
ATTGAGGTTAGGGTTATTCG+CGG 0.577972 8.2:-32233634 MS.gene93022:CDS
GGTTCCAGGTTTGGAATGAG+TGG 0.578526 8.2:+32234693 None:intergenic
GGTTTCAGATCTGAGTATGA+AGG 0.579176 8.2:-32234331 MS.gene93022:CDS
GTGGAAACCTTGCATTGGAA+AGG 0.581268 8.2:-32233672 MS.gene93022:CDS
GTGGATATGGTGGTGATTTG+GGG 0.583200 8.2:+32234735 None:intergenic
TAGTACATTTCCAAATAGAT+GGG 0.585798 8.2:-32232906 MS.gene93022:CDS
ATACTGGTGTTGCTATGACT+AGG 0.586035 8.2:-32232976 MS.gene93022:CDS
CTGCATAGATTATGGCATCC+CGG 0.587677 8.2:-32232648 MS.gene93022:CDS
GGATGCAGAAGTTGCAGTTG+TGG 0.588062 8.2:-32234133 MS.gene93022:CDS
CCAACATAAATTCCAGGTAT+CGG 0.588388 8.2:+32233937 None:intergenic
TAAATCTCAACCTTAATTTG+GGG 0.590545 8.2:-32234473 MS.gene93022:CDS
GTGTTTAGGTCTACACAGAC+AGG 0.591190 8.2:-32233052 MS.gene93022:CDS
TATGGTTGTATCGTGATCAA+AGG 0.592056 8.2:-32233969 MS.gene93022:CDS
TGTGATCAGAAATTGGAGGG+TGG 0.592511 8.2:-32233691 MS.gene93022:CDS
GTTTCAAGGGTTAATGGGAA+AGG 0.597617 8.2:+32234882 None:intergenic
GCCAGGTGATGGAACTTGTG+AGG 0.598287 8.2:-32234353 MS.gene93022:CDS
TGCAGAGTTCTCTCATAGGA+GGG 0.599457 8.2:+32232666 None:intergenic
TAATCTACACTGGCCATGGA+GGG 0.600090 8.2:-32233741 MS.gene93022:CDS
ACACGGTTTCGACAATTAGG+AGG 0.600465 8.2:+32233106 None:intergenic
GTGGAGGCAGAGATGAAAGT+GGG 0.600896 8.2:-32234114 MS.gene93022:CDS
ACTAGTGTAATTGTTTCAGG+TGG 0.601921 8.2:-32233802 MS.gene93022:CDS
AGGTTGGGGAGTTAGGTCCT+TGG 0.602845 8.2:-32233032 MS.gene93022:CDS
AGGTGGTTATGAGGATGACA+TGG 0.604358 8.2:-32233785 MS.gene93022:CDS
GGTGATATTATAATCTACAC+TGG 0.606082 8.2:-32233751 MS.gene93022:CDS
CCACATTCATGGTTATATGA+GGG 0.608512 8.2:-32234019 MS.gene93022:CDS
TTCGTCATAAGATAAGGAAG+AGG 0.608918 8.2:-32234296 MS.gene93022:CDS
ATTGCATATAACATGACATG+AGG 0.609949 8.2:+32232698 None:intergenic
ATTATGGCATCCCGGACGAG+TGG 0.613316 8.2:-32232640 MS.gene93022:CDS
TCTGATGAAACACAAACCAC+GGG 0.613935 8.2:+32233281 None:intergenic
ATTTGTGATCAGAAATTGGA+GGG 0.614502 8.2:-32233694 MS.gene93022:CDS
CAAATCACCACCATATCCAC+CGG 0.614581 8.2:-32234732 MS.gene93022:CDS
TTGCATATGCATATGCAAGC+TGG 0.615698 8.2:-32233874 MS.gene93022:CDS
GTTTGTGTTTCATCAGAGTG+GGG 0.619286 8.2:-32233275 MS.gene93022:CDS
CCCTCATATAACCATGAATG+TGG 0.621384 8.2:+32234019 None:intergenic
GGGTGAGGAGAATAGGGTGT+CGG 0.621767 8.2:-32234094 MS.gene93022:CDS
TCCAGCCTGAATAAGGTCCA+AGG 0.622985 8.2:+32233015 None:intergenic
ATAAGTTATTGAGAATTGAG+GGG 0.623555 8.2:-32233495 MS.gene93022:CDS
TTCGCGGGATGAAGTATGAA+GGG 0.624136 8.2:-32233618 MS.gene93022:CDS
TTATGGCATCCCGGACGAGT+GGG 0.624731 8.2:-32232639 MS.gene93022:CDS
ATAATCTACACTGGCCATGG+AGG 0.626398 8.2:-32233742 MS.gene93022:CDS
TTGAGGTTAGGGTTATTCGC+GGG 0.628964 8.2:-32233633 MS.gene93022:CDS
GGTGTCGGCTACCAACGTGG+AGG 0.632053 8.2:-32234079 MS.gene93022:CDS
TCCAGAAAATAATCTAAAGG+AGG 0.632885 8.2:+32232821 None:intergenic
TTAGGTCTACACAGACAGGT+TGG 0.633420 8.2:-32233048 MS.gene93022:CDS
TGTAAGGAGGCTTGTACTGA+CGG 0.637592 8.2:-32233235 MS.gene93022:CDS
TGCAGAAGTTGCAGTTGTGG+AGG 0.639345 8.2:-32234130 MS.gene93022:CDS
TGATGTTCCGGTGGATATGG+TGG 0.640019 8.2:+32234725 None:intergenic
GATGAGAAATACTAAGATCG+GGG 0.640700 8.2:+32234802 None:intergenic
TACGGTAAATGTTTGTAACC+CGG 0.645041 8.2:-32234499 MS.gene93022:CDS
GCTTCGCGTGAGAATTGAGG+AGG 0.645275 8.2:-32234256 MS.gene93022:CDS
AAATCTCAACCTTAATTTGG+GGG 0.645557 8.2:-32234472 MS.gene93022:CDS
TTCCGGATACTACTTTCCCG+TGG 0.649132 8.2:-32233297 MS.gene93022:CDS
CGTTTGAAGTGATGTTCCGG+TGG 0.656180 8.2:+32234716 None:intergenic
CCAAATAGATGGGTAGAATG+GGG 0.657480 8.2:-32232896 MS.gene93022:CDS
TCCTTACATTCACAACCACT+AGG 0.658950 8.2:+32233250 None:intergenic
CATCAGAGTGGGGAACCTAG+TGG 0.659288 8.2:-32233265 MS.gene93022:CDS
TTGCATATAACATGACATGA+GGG 0.662482 8.2:+32232699 None:intergenic
GGCGAGAGAAGAATTCCTCA+AGG 0.664502 8.2:-32233720 MS.gene93022:CDS
GAAACCGTGTTGCACAAAAG+GGG 0.665958 8.2:-32233093 MS.gene93022:CDS
GCGTCATTAATGAAGGAGCG+TGG 0.669061 8.2:-32233994 MS.gene93022:CDS
TGTGGAGGCAGAGATGAAAG+TGG 0.676329 8.2:-32234115 MS.gene93022:CDS
ATGAGAAATACTAAGATCGG+GGG 0.677362 8.2:+32234803 None:intergenic
TAGTGGTTGTGAATGTAAGG+AGG 0.682180 8.2:-32233248 MS.gene93022:CDS
TAGGGTGTCGGCTACCAACG+TGG 0.694009 8.2:-32234082 MS.gene93022:CDS
AATTTGTGATCAGAAATTGG+AGG 0.699714 8.2:-32233695 MS.gene93022:CDS
AAATGATCGAGCTATTGTTG+TGG 0.743063 8.2:-32234430 MS.gene93022:CDS
GATGACATGGAATTAGACGA+TGG 0.783210 8.2:-32233772 MS.gene93022:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGATATTTCAAATAATA+GGG - chr8.2:32234115-32234134 MS.gene93022:CDS 10.0%
!! CTATTGATATTTCAAATAAT+AGG - chr8.2:32234114-32234133 MS.gene93022:CDS 15.0%
!! ACATACTGAATGAATATATT+TGG + chr8.2:32234755-32234774 None:intergenic 20.0%
!! TATAAGTTATTGAGAATTGA+GGG - chr8.2:32234005-32234024 MS.gene93022:CDS 20.0%
!!! CTCAAATTTTAACAATGAAT+GGG - chr8.2:32234555-32234574 MS.gene93022:CDS 20.0%
!!! TCAAATTTTAACAATGAATG+GGG - chr8.2:32234556-32234575 MS.gene93022:CDS 20.0%
! ACATCCAGAAAATAATCTAA+AGG + chr8.2:32234686-32234705 None:intergenic 25.0%
! AGAAATAGAAGTAGAAGAAT+GGG + chr8.2:32232644-32232663 None:intergenic 25.0%
! ATAAGTTATTGAGAATTGAG+GGG - chr8.2:32234006-32234025 MS.gene93022:CDS 25.0%
! ATATCATTAAATAGCCTAAC+TGG + chr8.2:32234149-32234168 None:intergenic 25.0%
! CTAAATCTCAACCTTAATTT+GGG - chr8.2:32233027-32233046 MS.gene93022:CDS 25.0%
! CTAGGGATATTAAGAAAATT+GGG - chr8.2:32234063-32234082 MS.gene93022:CDS 25.0%
! GTATAAGTTATTGAGAATTG+AGG - chr8.2:32234004-32234023 MS.gene93022:CDS 25.0%
! TAGTACATTTCCAAATAGAT+GGG - chr8.2:32234595-32234614 MS.gene93022:CDS 25.0%
! TCTTTATTATGAGTATCTTC+CGG - chr8.2:32234187-32234206 MS.gene93022:CDS 25.0%
! TGAAACAACAAAATCATTGT+TGG + chr8.2:32232974-32232993 None:intergenic 25.0%
! TTGATGAGAAATACTAAGAT+CGG + chr8.2:32232704-32232723 None:intergenic 25.0%
!! GATTTAAGGTTGAAATTCTA+GGG + chr8.2:32232729-32232748 None:intergenic 25.0%
!! TAAATCTCAACCTTAATTTG+GGG - chr8.2:32233028-32233047 MS.gene93022:CDS 25.0%
!! TCAAAGAGTATGTCTTATAA+TGG - chr8.2:32233663-32233682 MS.gene93022:CDS 25.0%
!! TGATTTAAGGTTGAAATTCT+AGG + chr8.2:32232730-32232749 None:intergenic 25.0%
!!! AAACCTTTGATTTTTGAATG+CGG - chr8.2:32234356-32234375 MS.gene93022:CDS 25.0%
!!! CAATGATTTTGTTGTTTCAT+TGG - chr8.2:32232975-32232994 MS.gene93022:CDS 25.0%
!!! GCTCAAATTTTAACAATGAA+TGG - chr8.2:32234554-32234573 MS.gene93022:CDS 25.0%
AAGCAATCCATGTAGATTAT+AGG + chr8.2:32234327-32234346 None:intergenic 30.0%
AATGAATGAGGAGAAGAAAT+TGG - chr8.2:32233275-32233294 MS.gene93022:CDS 30.0%
AATTTGTGATCAGAAATTGG+AGG - chr8.2:32233806-32233825 MS.gene93022:CDS 30.0%
ACAATTACACTAGTAGCAAT+CGG + chr8.2:32233693-32233712 None:intergenic 30.0%
AGAAATTGTTAAAAGGTTCC+AGG + chr8.2:32232825-32232844 None:intergenic 30.0%
AGCAATCCATGTAGATTATA+GGG + chr8.2:32234326-32234345 None:intergenic 30.0%
ATATATTTGGACTCGAACTA+TGG + chr8.2:32234742-32234761 None:intergenic 30.0%
ATTGCATATAACATGACATG+AGG + chr8.2:32234806-32234825 None:intergenic 30.0%
ATTTGTGATCAGAAATTGGA+GGG - chr8.2:32233807-32233826 MS.gene93022:CDS 30.0%
CATCTATTTGGAAATGTACT+AGG + chr8.2:32234596-32234615 None:intergenic 30.0%
CCAAATTAAGGTTGAGATTT+AGG + chr8.2:32233029-32233048 None:intergenic 30.0%
CCTAAATCTCAACCTTAATT+TGG - chr8.2:32233026-32233045 MS.gene93022:CDS 30.0%
CTAGTACATTTCCAAATAGA+TGG - chr8.2:32234594-32234613 MS.gene93022:CDS 30.0%
GAGAAATAGAAGTAGAAGAA+TGG + chr8.2:32232645-32232664 None:intergenic 30.0%
GCAAATTTGTGATCAGAAAT+TGG - chr8.2:32233803-32233822 MS.gene93022:CDS 30.0%
GCTAGGGATATTAAGAAAAT+TGG - chr8.2:32234062-32234081 MS.gene93022:CDS 30.0%
GGAAGATACTCATAATAAAG+AGG + chr8.2:32234188-32234207 None:intergenic 30.0%
GGATATTAAGAAAATTGGGT+TGG - chr8.2:32234067-32234086 MS.gene93022:CDS 30.0%
GGTGATATTATAATCTACAC+TGG - chr8.2:32233750-32233769 MS.gene93022:CDS 30.0%
GTTAGTTGCTTGTTTGATAT+AGG - chr8.2:32233957-32233976 MS.gene93022:CDS 30.0%
TCAATAGAGATACAACTCAT+TGG + chr8.2:32234101-32234120 None:intergenic 30.0%
TCATGTCATGTTATATGCAA+TGG - chr8.2:32234805-32234824 MS.gene93022:CDS 30.0%
TCCAGAAAATAATCTAAAGG+AGG + chr8.2:32234683-32234702 None:intergenic 30.0%
TGATGAGAAATACTAAGATC+GGG + chr8.2:32232703-32232722 None:intergenic 30.0%
TTGCATATAACATGACATGA+GGG + chr8.2:32234805-32234824 None:intergenic 30.0%
TTGTTTGATATAGGAAAGTC+TGG - chr8.2:32233966-32233985 MS.gene93022:CDS 30.0%
! AAATCTCAACCTTAATTTGG+GGG - chr8.2:32233029-32233048 MS.gene93022:CDS 30.0%
! AATTGGTTGTTGAAGTTGAT+AGG - chr8.2:32233292-32233311 MS.gene93022:CDS 30.0%
! ACCTCCTTTAGATTATTTTC+TGG - chr8.2:32234679-32234698 MS.gene93022:CDS 30.0%
! AGAGAAATTAGGTTGTTGTT+GGG + chr8.2:32232863-32232882 None:intergenic 30.0%
!! AATATAGCTTGGAAGTGTTT+AGG - chr8.2:32234435-32234454 MS.gene93022:CDS 30.0%
!! ATTGGTTGTTGAAGTTGATA+GGG - chr8.2:32233293-32233312 MS.gene93022:CDS 30.0%
!! TAATCTAAAGGAGGTATTGA+CGG + chr8.2:32234674-32234693 None:intergenic 30.0%
!! TACATGGATTGCTTTTGAAA+GGG - chr8.2:32234333-32234352 MS.gene93022:CDS 30.0%
!! TAGAATTTGGTGTATAGGTT+GGG + chr8.2:32232910-32232929 None:intergenic 30.0%
!! TGATGTGTTTTTGTTTAGGA+GGG - chr8.2:32233587-32233606 MS.gene93022:CDS 30.0%
!! TGTTGTTTCATTGGAGAATA+CGG - chr8.2:32232984-32233003 MS.gene93022:CDS 30.0%
!!! TTGGTGATGTGTTTTTGTTT+AGG - chr8.2:32233583-32233602 MS.gene93022:CDS 30.0%
AAATGATCGAGCTATTGTTG+TGG - chr8.2:32233071-32233090 MS.gene93022:CDS 35.0%
AATCAGCGATTTCGAGAAAA+GGG + chr8.2:32232951-32232970 None:intergenic 35.0%
ACATCACCAACATAAATTCC+AGG + chr8.2:32233573-32233592 None:intergenic 35.0%
ACCACATTCATGGTTATATG+AGG - chr8.2:32233481-32233500 MS.gene93022:CDS 35.0%
ACTAGTGTAATTGTTTCAGG+TGG - chr8.2:32233699-32233718 MS.gene93022:CDS 35.0%
AGAGAACTCTGCATAGATTA+TGG - chr8.2:32234845-32234864 MS.gene93022:CDS 35.0%
ATAGGGAAAATGTTCCAGTT+AGG - chr8.2:32234132-32234151 MS.gene93022:CDS 35.0%
ATGAGAAATACTAAGATCGG+GGG + chr8.2:32232701-32232720 None:intergenic 35.0%
ATTATAATCTACACTGGCCA+TGG - chr8.2:32233756-32233775 MS.gene93022:CDS 35.0%
ATTGGATGAGGAGAAGATAT+TGG - chr8.2:32233347-32233366 MS.gene93022:CDS 35.0%
ATTGTCTGAGGAAAGGAAAT+TGG - chr8.2:32233329-32233348 MS.gene93022:CDS 35.0%
CCAACATAAATTCCAGGTAT+CGG + chr8.2:32233567-32233586 None:intergenic 35.0%
CCACATTCATGGTTATATGA+GGG - chr8.2:32233482-32233501 MS.gene93022:CDS 35.0%
GACGCGAAGAAATTGTTAAA+AGG + chr8.2:32232832-32232851 None:intergenic 35.0%
GAGTTTCCCTATAATCTACA+TGG - chr8.2:32234317-32234336 MS.gene93022:CDS 35.0%
GATGAGAAATACTAAGATCG+GGG + chr8.2:32232702-32232721 None:intergenic 35.0%
GCTACTAGTGTAATTGTTTC+AGG - chr8.2:32233696-32233715 MS.gene93022:CDS 35.0%
GGGAGACAATTAATGGAATA+AGG - chr8.2:32233445-32233464 MS.gene93022:CDS 35.0%
GGTAAGGTTTATGTTTACGA+TGG - chr8.2:32233921-32233940 MS.gene93022:CDS 35.0%
TACGGTAAATGTTTGTAACC+CGG - chr8.2:32233002-32233021 MS.gene93022:CDS 35.0%
TATCGTGATCAAAGGATAGT+TGG - chr8.2:32233540-32233559 MS.gene93022:CDS 35.0%
TATGGTTGTATCGTGATCAA+AGG - chr8.2:32233532-32233551 MS.gene93022:CDS 35.0%
TCCAAATAGATGGGTAGAAT+GGG - chr8.2:32234604-32234623 MS.gene93022:CDS 35.0%
TGCACTTTCGTCATAAGATA+AGG - chr8.2:32233199-32233218 MS.gene93022:CDS 35.0%
TTAAGGTTGAGATTTAGGTC+CGG + chr8.2:32233024-32233043 None:intergenic 35.0%
TTCCAAATAGATGGGTAGAA+TGG - chr8.2:32234603-32234622 MS.gene93022:CDS 35.0%
TTCGTCATAAGATAAGGAAG+AGG - chr8.2:32233205-32233224 MS.gene93022:CDS 35.0%
TTGTTAAAAGGTTCCAGGTT+TGG + chr8.2:32232820-32232839 None:intergenic 35.0%
! AAGTAGAAGAATGGGTTTCA+AGG + chr8.2:32232636-32232655 None:intergenic 35.0%
! AATGGGTTTCAAGGGTTAAT+GGG + chr8.2:32232627-32232646 None:intergenic 35.0%
! AATTGTTTCAGGTGGTTATG+AGG - chr8.2:32233707-32233726 MS.gene93022:CDS 35.0%
! AGTAGAAGAATGGGTTTCAA+GGG + chr8.2:32232635-32232654 None:intergenic 35.0%
! GAGAGAAATTAGGTTGTTGT+TGG + chr8.2:32232864-32232883 None:intergenic 35.0%
! TTTGATGGCAGATATGGTTA+TGG - chr8.2:32233116-32233135 MS.gene93022:CDS 35.0%
!! CTACATGGATTGCTTTTGAA+AGG - chr8.2:32234332-32234351 MS.gene93022:CDS 35.0%
!! GATATAGGAAAGTCTGGTTT+TGG - chr8.2:32233972-32233991 MS.gene93022:CDS 35.0%
!! GTAGAATTTGGTGTATAGGT+TGG + chr8.2:32232911-32232930 None:intergenic 35.0%
!! GTGATGTGTTTTTGTTTAGG+AGG - chr8.2:32233586-32233605 MS.gene93022:CDS 35.0%
!! TGTGGTAGAATTTGGTGTAT+AGG + chr8.2:32232915-32232934 None:intergenic 35.0%
!! TTGATCATCGGTTTCAGATT+CGG + chr8.2:32232887-32232906 None:intergenic 35.0%
!!! TTTTGAGGTGTGGTAGAATT+TGG + chr8.2:32232923-32232942 None:intergenic 35.0%
AAAGGGGCTCAAATATAGCT+TGG - chr8.2:32234424-32234443 MS.gene93022:CDS 40.0%
ACCAGTATACTCGCAGATAA+AGG + chr8.2:32234513-32234532 None:intergenic 40.0%
ACCTAGTGGTTGTGAATGTA+AGG - chr8.2:32234250-32234269 MS.gene93022:CDS 40.0%
ACTCGAACTATGGCTCATAT+AGG + chr8.2:32234732-32234751 None:intergenic 40.0%
AGAAGAGATTGTCTGAGGAA+AGG - chr8.2:32233322-32233341 MS.gene93022:CDS 40.0%
ATTGAGGTTAGGGTTATTCG+CGG - chr8.2:32233867-32233886 MS.gene93022:CDS 40.0%
CACTGTTCTCAAAGATGCTA+GGG - chr8.2:32234046-32234065 MS.gene93022:CDS 40.0%
CCAAATAGATGGGTAGAATG+GGG - chr8.2:32234605-32234624 MS.gene93022:CDS 40.0%
CCCTCATATAACCATGAATG+TGG + chr8.2:32233485-32233504 None:intergenic 40.0%
CCGATACCTGGAATTTATGT+TGG - chr8.2:32233564-32233583 MS.gene93022:CDS 40.0%
CGTAGTATCAACCACATTCA+TGG - chr8.2:32233471-32233490 MS.gene93022:CDS 40.0%
CTCTGATGAAACACAAACCA+CGG + chr8.2:32234224-32234243 None:intergenic 40.0%
CTGATCACAAATTTGCCTTG+AGG + chr8.2:32233799-32233818 None:intergenic 40.0%
GAAACAAGAGTCGAGTTTGA+TGG - chr8.2:32233101-32233120 MS.gene93022:CDS 40.0%
GAATCAGCGATTTCGAGAAA+AGG + chr8.2:32232952-32232971 None:intergenic 40.0%
GATGACATGGAATTAGACGA+TGG - chr8.2:32233729-32233748 MS.gene93022:CDS 40.0%
GGAATAGGAACATGAGAAGA+AGG + chr8.2:32232670-32232689 None:intergenic 40.0%
GGTTTCAGATCTGAGTATGA+AGG - chr8.2:32233170-32233189 MS.gene93022:CDS 40.0%
GGTTTGTGTTTCATCAGAGT+GGG - chr8.2:32234225-32234244 MS.gene93022:CDS 40.0%
GTTTCAAGGGTTAATGGGAA+AGG + chr8.2:32232622-32232641 None:intergenic 40.0%
GTTTGTGTTTCATCAGAGTG+GGG - chr8.2:32234226-32234245 MS.gene93022:CDS 40.0%
TAAAGGATCCAGCCTGAATA+AGG + chr8.2:32234496-32234515 None:intergenic 40.0%
TAAGGTTGAGATTTAGGTCC+GGG + chr8.2:32233023-32233042 None:intergenic 40.0%
TAGGAGGACATGAGCAAAAA+GGG + chr8.2:32234382-32234401 None:intergenic 40.0%
TAGTGGTTGTGAATGTAAGG+AGG - chr8.2:32234253-32234272 MS.gene93022:CDS 40.0%
TCAGATTCGGGAGAGAAATT+AGG + chr8.2:32232874-32232893 None:intergenic 40.0%
TCCTTACATTCACAACCACT+AGG + chr8.2:32234254-32234273 None:intergenic 40.0%
TCCTTTATCTGCGAGTATAC+TGG - chr8.2:32234509-32234528 MS.gene93022:CDS 40.0%
TCTATGCAGAGTTCTCTCAT+AGG + chr8.2:32234842-32234861 None:intergenic 40.0%
TCTGATGAAACACAAACCAC+GGG + chr8.2:32234223-32234242 None:intergenic 40.0%
TGAGGAAAGGAAATTGGATG+AGG - chr8.2:32233335-32233354 MS.gene93022:CDS 40.0%
TTAGGAGGACATGAGCAAAA+AGG + chr8.2:32234383-32234402 None:intergenic 40.0%
TTGCATATGCATATGCAAGC+TGG - chr8.2:32233627-32233646 MS.gene93022:CDS 40.0%
! AAACTCGACTCTTGTTTCTC+AGG + chr8.2:32233099-32233118 None:intergenic 40.0%
! AGGTTGTTGTTGGGAATCTT+GGG + chr8.2:32232854-32232873 None:intergenic 40.0%
! GAATGGGTTTCAAGGGTTAA+TGG + chr8.2:32232628-32232647 None:intergenic 40.0%
! GAGAAAAGGGTAGAGTTTTG+AGG + chr8.2:32232938-32232957 None:intergenic 40.0%
! TAGGTTGTTGTTGGGAATCT+TGG + chr8.2:32232855-32232874 None:intergenic 40.0%
! TGCTTTTGCGCTATGAAGAA+TGG - chr8.2:32234290-32234309 MS.gene93022:CDS 40.0%
! TGGAAAGGAGTATGCACTAT+GGG - chr8.2:32233844-32233863 MS.gene93022:CDS 40.0%
! TGGTTTGTGTTTCATCAGAG+TGG - chr8.2:32234224-32234243 MS.gene93022:CDS 40.0%
! TTGGAAAGGAGTATGCACTA+TGG - chr8.2:32233843-32233862 MS.gene93022:CDS 40.0%
! TTTTGCGCTATGAAGAATGG+AGG - chr8.2:32234293-32234312 MS.gene93022:CDS 40.0%
!! ATACTGGTGTTGCTATGACT+AGG - chr8.2:32234525-32234544 MS.gene93022:CDS 40.0%
!! GTATAGGTTGGGTTGATCAT+CGG + chr8.2:32232899-32232918 None:intergenic 40.0%
!! TACTGGTGTTGCTATGACTA+GGG - chr8.2:32234526-32234545 MS.gene93022:CDS 40.0%
!! TGATCATCGGTTTCAGATTC+GGG + chr8.2:32232886-32232905 None:intergenic 40.0%
AACAATGAATGGGGTCTCGT+TGG - chr8.2:32234565-32234584 MS.gene93022:CDS 45.0%
AACCACGGGAAAGTAGTATC+CGG + chr8.2:32234209-32234228 None:intergenic 45.0%
AAGTGATGTTCCGGTGGATA+TGG + chr8.2:32232782-32232801 None:intergenic 45.0%
ACACGGTTTCGACAATTAGG+AGG + chr8.2:32234398-32234417 None:intergenic 45.0%
AGGTGGTTATGAGGATGACA+TGG - chr8.2:32233716-32233735 MS.gene93022:CDS 45.0%
ATAATCTACACTGGCCATGG+AGG - chr8.2:32233759-32233778 MS.gene93022:CDS 45.0%
ATGCAGAGTTCTCTCATAGG+AGG + chr8.2:32234839-32234858 None:intergenic 45.0%
ATTCGCGGGATGAAGTATGA+AGG - chr8.2:32233882-32233901 MS.gene93022:CDS 45.0%
CAAATCACCACCATATCCAC+CGG - chr8.2:32232769-32232788 MS.gene93022:CDS 45.0%
CAGAATCCACCCCCAAATTA+AGG + chr8.2:32233041-32233060 None:intergenic 45.0%
CCCCATTCTACCCATCTATT+TGG + chr8.2:32234608-32234627 None:intergenic 45.0%
CGAAACCGTGTTGCACAAAA+GGG - chr8.2:32234407-32234426 MS.gene93022:CDS 45.0%
CTGCATAGATTATGGCATCC+CGG - chr8.2:32234853-32234872 MS.gene93022:CDS 45.0%
GAAACCGTGTTGCACAAAAG+GGG - chr8.2:32234408-32234427 MS.gene93022:CDS 45.0%
GAAAGTGGGTGAGGAGAATA+GGG - chr8.2:32233401-32233420 MS.gene93022:CDS 45.0%
GAGTATGCACTATGGGATTG+AGG - chr8.2:32233851-32233870 MS.gene93022:CDS 45.0%
GAGTGCATCTACTTCTGGTA+AGG - chr8.2:32233905-32233924 MS.gene93022:CDS 45.0%
GCAACACGGTTTCGACAATT+AGG + chr8.2:32234401-32234420 None:intergenic 45.0%
GCACTATGGGATTGAGGTTA+GGG - chr8.2:32233857-32233876 MS.gene93022:CDS 45.0%
GCACTGTTCTCAAAGATGCT+AGG - chr8.2:32234045-32234064 MS.gene93022:CDS 45.0%
GCATACTCCTTTCCAATGCA+AGG + chr8.2:32233839-32233858 None:intergenic 45.0%
GGAAGAGAAGAGATTGTCTG+AGG - chr8.2:32233317-32233336 MS.gene93022:CDS 45.0%
GGAGGACAAGAGAATGAATG+AGG - chr8.2:32233263-32233282 MS.gene93022:CDS 45.0%
GGCAGATATGGTTATGGAGA+AGG - chr8.2:32233122-32233141 MS.gene93022:CDS 45.0%
GGGCCGCATTCAAAAATCAA+AGG + chr8.2:32234362-32234381 None:intergenic 45.0%
GTGGAAACCTTGCATTGGAA+AGG - chr8.2:32233829-32233848 MS.gene93022:CDS 45.0%
GTGTTTAGGTCTACACAGAC+AGG - chr8.2:32234449-32234468 MS.gene93022:CDS 45.0%
GTTATTGAGAATTGAGGGGC+AGG - chr8.2:32234010-32234029 MS.gene93022:CDS 45.0%
TAATCTACACTGGCCATGGA+GGG - chr8.2:32233760-32233779 MS.gene93022:CDS 45.0%
TAGGTCCTTGGACCTTATTC+AGG - chr8.2:32234481-32234500 MS.gene93022:CDS 45.0%
TAGGTCTACACAGACAGGTT+GGG - chr8.2:32234454-32234473 MS.gene93022:CDS 45.0%
TCGAAACCGTGTTGCACAAA+AGG - chr8.2:32234406-32234425 MS.gene93022:CDS 45.0%
TGAAAGTGGGTGAGGAGAAT+AGG - chr8.2:32233400-32233419 MS.gene93022:CDS 45.0%
TGCACTATGGGATTGAGGTT+AGG - chr8.2:32233856-32233875 MS.gene93022:CDS 45.0%
TGCAGAGTTCTCTCATAGGA+GGG + chr8.2:32234838-32234857 None:intergenic 45.0%
TGTAAGGAGGCTTGTACTGA+CGG - chr8.2:32234266-32234285 MS.gene93022:CDS 45.0%
TGTGATCAGAAATTGGAGGG+TGG - chr8.2:32233810-32233829 MS.gene93022:CDS 45.0%
TTAGGTCTACACAGACAGGT+TGG - chr8.2:32234453-32234472 MS.gene93022:CDS 45.0%
TTCGCGGGATGAAGTATGAA+GGG - chr8.2:32233883-32233902 MS.gene93022:CDS 45.0%
TTGAGGTTAGGGTTATTCGC+GGG - chr8.2:32233868-32233887 MS.gene93022:CDS 45.0%
! ATATGGTGGTGATTTGGGGT+GGG + chr8.2:32232765-32232784 None:intergenic 45.0%
! GGGGTGGATTCTGTAAATGA+TGG - chr8.2:32233048-32233067 MS.gene93022:CDS 45.0%
! GGTGGATATGGTGGTGATTT+GGG + chr8.2:32232770-32232789 None:intergenic 45.0%
! GTCGAGTTTGATGGCAGATA+TGG - chr8.2:32233110-32233129 MS.gene93022:CDS 45.0%
! GTGGATATGGTGGTGATTTG+GGG + chr8.2:32232769-32232788 None:intergenic 45.0%
! TAATGAAGGAGCGTGGACTA+TGG - chr8.2:32233514-32233533 MS.gene93022:CDS 45.0%
! TCTCAACCTTAATTTGGGGG+TGG - chr8.2:32233032-32233051 MS.gene93022:CDS 45.0%
! TGGCGTTTGAAGTGATGTTC+CGG + chr8.2:32232791-32232810 None:intergenic 45.0%
!! GGAGGTATTGACGGATAAGA+TGG + chr8.2:32234665-32234684 None:intergenic 45.0%
!! GGTTGTTGAAGTTGATAGGG+AGG - chr8.2:32233296-32233315 MS.gene93022:CDS 45.0%
!!! AAGGGTAGAGTTTTGAGGTG+TGG + chr8.2:32232933-32232952 None:intergenic 45.0%
AACGCCACTCATTCCAAACC+TGG - chr8.2:32232804-32232823 MS.gene93022:CDS 50.0%
AATTCTTCTCTCGCCCTCCA+TGG + chr8.2:32233776-32233795 None:intergenic 50.0%
AGGAGGGAATTGTGTGTGAC+TGG - chr8.2:32233603-32233622 MS.gene93022:CDS 50.0%
AGGATAGTTGGACCGATACC+TGG - chr8.2:32233552-32233571 MS.gene93022:CDS 50.0%
AGGTCTACACAGACAGGTTG+GGG - chr8.2:32234455-32234474 MS.gene93022:CDS 50.0%
CTAAGATCGGGGGTGATAGT+AGG + chr8.2:32232691-32232710 None:intergenic 50.0%
GAGGGCTGCGTCATTAATGA+AGG - chr8.2:32233500-32233519 MS.gene93022:CDS 50.0%
GCGTCATTAATGAAGGAGCG+TGG - chr8.2:32233507-32233526 MS.gene93022:CDS 50.0%
GGATGCAGAAGTTGCAGTTG+TGG - chr8.2:32233368-32233387 MS.gene93022:CDS 50.0%
GGCGAGAGAAGAATTCCTCA+AGG - chr8.2:32233781-32233800 MS.gene93022:CDS 50.0%
GGTGAGAGCGGTGTGATTTA+AGG + chr8.2:32232743-32232762 None:intergenic 50.0%
GGTTATGGAGAAGGAAAGCC+AGG - chr8.2:32233131-32233150 MS.gene93022:CDS 50.0%
GTGGAGGCAGAGATGAAAGT+GGG - chr8.2:32233387-32233406 MS.gene93022:CDS 50.0%
GTGGAGGGGGAGACAATTAA+TGG - chr8.2:32233438-32233457 MS.gene93022:CDS 50.0%
TCCAGCCTGAATAAGGTCCA+AGG + chr8.2:32234489-32234508 None:intergenic 50.0%
TCCTTGGACCTTATTCAGGC+TGG - chr8.2:32234485-32234504 MS.gene93022:CDS 50.0%
TCGGGGGTGATAGTAGGAAT+AGG + chr8.2:32232685-32232704 None:intergenic 50.0%
TGATGTTCCGGTGGATATGG+TGG + chr8.2:32232779-32232798 None:intergenic 50.0%
TGCAGAAGTTGCAGTTGTGG+AGG - chr8.2:32233371-32233390 MS.gene93022:CDS 50.0%
TGTGGAGGCAGAGATGAAAG+TGG - chr8.2:32233386-32233405 MS.gene93022:CDS 50.0%
TTCCGGATACTACTTTCCCG+TGG - chr8.2:32234204-32234223 MS.gene93022:CDS 50.0%
TTCGCTTCGCGTGAGAATTG+AGG - chr8.2:32233242-32233261 MS.gene93022:CDS 50.0%
! CGGTGGATATGGTGGTGATT+TGG + chr8.2:32232771-32232790 None:intergenic 50.0%
! CGTTTGAAGTGATGTTCCGG+TGG + chr8.2:32232788-32232807 None:intergenic 50.0%
! GAAGGGAGTGCATCTACTTC+TGG - chr8.2:32233900-32233919 MS.gene93022:CDS 50.0%
! GATATGGTGGTGATTTGGGG+TGG + chr8.2:32232766-32232785 None:intergenic 50.0%
! GGTTCCAGGTTTGGAATGAG+TGG + chr8.2:32232811-32232830 None:intergenic 50.0%
! TATGGTGGTGATTTGGGGTG+GGG + chr8.2:32232764-32232783 None:intergenic 50.0%
! TGAGCCCCTTTTGTGCAACA+CGG + chr8.2:32234415-32234434 None:intergenic 50.0%
!! CCAGGTGATGGAACTTGTGA+GGG - chr8.2:32233149-32233168 MS.gene93022:CDS 50.0%
AATTGAGGGGCAGGCGAAGA+TGG - chr8.2:32234019-32234038 MS.gene93022:CDS 55.0%
AATTGTCTCCCCCTCCACGT+TGG + chr8.2:32233436-32233455 None:intergenic 55.0%
AGGTTGGGGAGTTAGGTCCT+TGG - chr8.2:32234469-32234488 MS.gene93022:CDS 55.0%
ATTATGGCATCCCGGACGAG+TGG - chr8.2:32234861-32234880 MS.gene93022:CDS 55.0%
ATTGAGGGGCAGGCGAAGAT+GGG - chr8.2:32234020-32234039 MS.gene93022:CDS 55.0%
CATCAGAGTGGGGAACCTAG+TGG - chr8.2:32234236-32234255 MS.gene93022:CDS 55.0%
CCCTCACAAGTTCCATCACC+TGG + chr8.2:32233152-32233171 None:intergenic 55.0%
GCTTCGCGTGAGAATTGAGG+AGG - chr8.2:32233245-32233264 MS.gene93022:CDS 55.0%
GGAGAAGGAAAGCCAGGTGA+TGG - chr8.2:32233137-32233156 MS.gene93022:CDS 55.0%
GGAGGGTGGAAACCTTGCAT+TGG - chr8.2:32233824-32233843 MS.gene93022:CDS 55.0%
GGCAGAGATGAAAGTGGGTG+AGG - chr8.2:32233392-32233411 MS.gene93022:CDS 55.0%
GGGTGAGGAGAATAGGGTGT+CGG - chr8.2:32233407-32233426 MS.gene93022:CDS 55.0%
TTATGGCATCCCGGACGAGT+GGG - chr8.2:32234862-32234881 MS.gene93022:CDS 55.0%
! CACAGACAGGTTGGGGAGTT+AGG - chr8.2:32234462-32234481 MS.gene93022:CDS 55.0%
!! GCCAGGTGATGGAACTTGTG+AGG - chr8.2:32233148-32233167 MS.gene93022:CDS 55.0%
ATCTTGCTCGCCCACTCGTC+CGG + chr8.2:32234875-32234894 None:intergenic 60.0%
! GATTTGGGGTGGGGTGAGAG+CGG + chr8.2:32232755-32232774 None:intergenic 60.0%
! TGTCGGCTACCAACGTGGAG+GGG - chr8.2:32233424-32233443 MS.gene93022:CDS 60.0%
!! GTGTCGGCTACCAACGTGGA+GGG - chr8.2:32233423-32233442 MS.gene93022:CDS 60.0%
!! TAGGGTGTCGGCTACCAACG+TGG - chr8.2:32233419-32233438 MS.gene93022:CDS 60.0%
GTCGGCTACCAACGTGGAGG+GGG - chr8.2:32233425-32233444 MS.gene93022:CDS 65.0%
TCTTGCTCGCCCACTCGTCC+GGG + chr8.2:32234874-32234893 None:intergenic 65.0%
!! GGTGTCGGCTACCAACGTGG+AGG - chr8.2:32233422-32233441 MS.gene93022:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 32232613 32234910 32232613 ID=MS.gene93022
chr8.2 mRNA 32232613 32234910 32232613 ID=MS.gene93022.t1;Parent=MS.gene93022
chr8.2 exon 32232613 32234910 32232613 ID=MS.gene93022.t1.exon1;Parent=MS.gene93022.t1
chr8.2 CDS 32232613 32234910 32232613 ID=cds.MS.gene93022.t1;Parent=MS.gene93022.t1
Gene Sequence

>MS.gene93022

ATGGATCCTTTCCCATTAACCCTTGAAACCCATTCTTCTACTTCTATTTCTCAACCTTCTTCTCATGTTCCTATTCCTACTATCACCCCCGATCTTAGTATTTCTCATCAAATCCCTAGAATTTCAACCTTAAATCACACCGCTCTCACCCCACCCCAAATCACCACCATATCCACCGGAACATCACTTCAAACGCCACTCATTCCAAACCTGGAACCTTTTAACAATTTCTTCGCGTCCCAAGATTCCCAACAACAACCTAATTTCTCTCCCGAATCTGAAACCGATGATCAACCCAACCTATACACCAAATTCTACCACACCTCAAAACTCTACCCTTTTCTCGAAATCGCTGATTCCAACAATGATTTTGTTGTTTCATTGGAGAATACGGTAAATGTTTGTAACCCGGACCTAAATCTCAACCTTAATTTGGGGGTGGATTCTGTAAATGATGGAAATGATCGAGCTATTGTTGTGGCTCCTGAGAAACAAGAGTCGAGTTTGATGGCAGATATGGTTATGGAGAAGGAAAGCCAGGTGATGGAACTTGTGAGGGTTTCAGATCTGAGTATGAAGGATCAGATGCACTTTCGTCATAAGATAAGGAAGAGGCGAATGATTTATGATTCGCTTCGCGTGAGAATTGAGGAGGACAAGAGAATGAATGAGGAGAAGAAATTGGTTGTTGAAGTTGATAGGGAGGAAGAGAAGAGATTGTCTGAGGAAAGGAAATTGGATGAGGAGAAGATATTGGATGCAGAAGTTGCAGTTGTGGAGGCAGAGATGAAAGTGGGTGAGGAGAATAGGGTGTCGGCTACCAACGTGGAGGGGGAGACAATTAATGGAATAAGGCGTCGTAGTATCAACCACATTCATGGTTATATGAGGGCTGCGTCATTAATGAAGGAGCGTGGACTATGGTTGTATCGTGATCAAAGGATAGTTGGACCGATACCTGGAATTTATGTTGGTGATGTGTTTTTGTTTAGGAGGGAATTGTGTGTGACTGGTTTGCATATGCATATGCAAGCTGGTATTGATTATCTATCAAAGAGTATGTCTTATAATGGTGAACCGATTGCTACTAGTGTAATTGTTTCAGGTGGTTATGAGGATGACATGGAATTAGACGATGGTGATATTATAATCTACACTGGCCATGGAGGGCGAGAGAAGAATTCCTCAAGGCAAATTTGTGATCAGAAATTGGAGGGTGGAAACCTTGCATTGGAAAGGAGTATGCACTATGGGATTGAGGTTAGGGTTATTCGCGGGATGAAGTATGAAGGGAGTGCATCTACTTCTGGTAAGGTTTATGTTTACGATGGATTGTATAAGATTGTTAGTTGCTTGTTTGATATAGGAAAGTCTGGTTTTGGAGTTTATAAGTATAAGTTATTGAGAATTGAGGGGCAGGCGAAGATGGGTAGCACTGTTCTCAAAGATGCTAGGGATATTAAGAAAATTGGGTTGGATTTCATGCCAATGAGTTGTATCTCTATTGATATTTCAAATAATAGGGAAAATGTTCCAGTTAGGCTATTTAATGATATAGATGTCAATCATGAACCTCTTTATTATGAGTATCTTCCGGATACTACTTTCCCGTGGTTTGTGTTTCATCAGAGTGGGGAACCTAGTGGTTGTGAATGTAAGGAGGCTTGTACTGACGGTTGCTTTTGCGCTATGAAGAATGGAGGAGAGTTTCCCTATAATCTACATGGATTGCTTTTGAAAGGGAAACCTTTGATTTTTGAATGCGGCCCTTTTTGCTCATGTCCTCCTAATTGTCGAAACCGTGTTGCACAAAAGGGGCTCAAATATAGCTTGGAAGTGTTTAGGTCTACACAGACAGGTTGGGGAGTTAGGTCCTTGGACCTTATTCAGGCTGGATCCTTTATCTGCGAGTATACTGGTGTTGCTATGACTAGGGAACAAGCTCAAATTTTAACAATGAATGGGGTCTCGTTGGTGTATCCTAGTACATTTCCAAATAGATGGGTAGAATGGGGTGATTTATCTCAGATATACACTGAATATGTGCGACCATCTTATCCGTCAATACCTCCTTTAGATTATTTTCTGGATGTGTCAACAATGAGAAATGTTGCATCCTATATGAGCCATAGTTCGAGTCCAAATATATTCATTCAGTATGTTCTTTATGATCACAACAATTTGTTGTTCCCTCATGTCATGTTATATGCAATGGAGAACATCCCTCCTATGAGAGAACTCTGCATAGATTATGGCATCCCGGACGAGTGGGCGAGCAAGATGTCTATATGTATGTAA
Protein sequence

>MS.gene93022.t1

MDPFPLTLETHSSTSISQPSSHVPIPTITPDLSISHQIPRISTLNHTALTPPQITTISTGTSLQTPLIPNLEPFNNFFASQDSQQQPNFSPESETDDQPNLYTKFYHTSKLYPFLEIADSNNDFVVSLENTVNVCNPDLNLNLNLGVDSVNDGNDRAIVVAPEKQESSLMADMVMEKESQVMELVRVSDLSMKDQMHFRHKIRKRRMIYDSLRVRIEEDKRMNEEKKLVVEVDREEEKRLSEERKLDEEKILDAEVAVVEAEMKVGEENRVSATNVEGETINGIRRRSINHIHGYMRAASLMKERGLWLYRDQRIVGPIPGIYVGDVFLFRRELCVTGLHMHMQAGIDYLSKSMSYNGEPIATSVIVSGGYEDDMELDDGDIIIYTGHGGREKNSSRQICDQKLEGGNLALERSMHYGIEVRVIRGMKYEGSASTSGKVYVYDGLYKIVSCLFDIGKSGFGVYKYKLLRIEGQAKMGSTVLKDARDIKKIGLDFMPMSCISIDISNNRENVPVRLFNDIDVNHEPLYYEYLPDTTFPWFVFHQSGEPSGCECKEACTDGCFCAMKNGGEFPYNLHGLLLKGKPLIFECGPFCSCPPNCRNRVAQKGLKYSLEVFRSTQTGWGVRSLDLIQAGSFICEYTGVAMTREQAQILTMNGVSLVYPSTFPNRWVEWGDLSQIYTEYVRPSYPSIPPLDYFLDVSTMRNVASYMSHSSSPNIFIQYVLYDHNNLLFPHVMLYAMENIPPMRELCIDYGIPDEWASKMSICM