AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93060.t1 MTR_8g070075 88.743 764 80 1 1 764 1 758 0.0 1414
MS.gene93060.t1 MTR_8g070070 72.994 785 176 9 4 768 1 769 0.0 1123
MS.gene93060.t1 MTR_8g071030 63.145 795 217 9 4 768 1 749 0.0 971
MS.gene93060.t1 MTR_1g035420 66.736 481 156 3 288 767 181 658 0.0 685
MS.gene93060.t1 MTR_1g035400 59.619 473 188 3 288 759 98 568 0.0 590
MS.gene93060.t1 MTR_6g044690 52.855 543 221 13 228 756 43 564 0.0 545
MS.gene93060.t1 MTR_0376s0030 52.881 486 204 7 293 767 106 577 1.53e-175 516
MS.gene93060.t1 MTR_6g043960 48.659 522 219 11 244 756 1 482 1.65e-159 471
MS.gene93060.t1 MTR_2g054460 44.035 570 201 12 196 756 4 464 1.88e-141 424
MS.gene93060.t1 MTR_5g013420 41.037 463 250 8 296 754 615 1058 1.01e-108 357
MS.gene93060.t1 MTR_5g013420 41.037 463 250 8 296 754 615 1058 1.07e-108 357
MS.gene93060.t1 MTR_4g010830 42.208 462 245 8 296 754 507 949 9.33e-106 347
MS.gene93060.t1 MTR_7g088370 40.977 471 242 13 296 756 236 680 9.99e-105 337
MS.gene93060.t1 MTR_3g011440 40.000 475 255 13 296 755 174 633 3.18e-102 329
MS.gene93060.t1 MTR_8g042750 38.987 454 249 11 316 756 220 658 2.68e-96 314
MS.gene93060.t1 MTR_4g010550 38.961 462 230 11 296 754 21 433 5.19e-91 293
MS.gene93060.t1 MTR_7g084090 37.882 491 267 15 289 755 251 727 1.30e-89 298
MS.gene93060.t1 MTR_6g061270 38.750 480 263 16 296 755 196 664 7.28e-88 292
MS.gene93060.t1 MTR_6g061200 37.634 465 247 16 302 755 203 635 3.70e-82 276
MS.gene93060.t1 MTR_7g084090 40.541 370 198 13 289 645 251 611 8.00e-72 248
MS.gene93060.t1 MTR_7g445710 32.773 476 227 18 299 754 110 512 2.67e-55 199
MS.gene93060.t1 MTR_7g445530 32.563 476 228 18 299 754 110 512 3.79e-55 198
MS.gene93060.t1 MTR_7g450730 30.882 476 216 15 293 754 98 474 1.00e-52 191
MS.gene93060.t1 MTR_1g048950 33.053 475 269 13 295 754 67 507 3.49e-52 191
MS.gene93060.t1 MTR_7g052480 36.154 260 153 7 506 755 270 526 3.01e-42 162
MS.gene93060.t1 MTR_8g078505 25.749 334 195 10 452 754 204 515 7.27e-22 100
MS.gene93060.t1 MTR_7g445600 34.000 150 62 2 605 754 243 355 9.45e-21 95.1
MS.gene93060.t1 MTR_7g445600 41.860 129 61 5 299 427 106 220 5.25e-16 80.9
MS.gene93060.t1 MTR_1g069570 26.710 307 153 12 506 756 430 720 3.40e-20 96.3
MS.gene93060.t1 MTR_6g044000 38.400 125 72 2 244 368 1 120 7.10e-20 88.2
MS.gene93060.t1 MTR_6g044000 69.811 53 16 0 704 756 117 169 1.94e-19 86.7
MS.gene93060.t1 MTR_8g078490 31.217 189 110 4 571 754 1056 1229 9.55e-20 95.5
MS.gene93060.t1 MTR_4g132610 30.270 185 115 4 572 754 851 1023 1.21e-18 91.7
MS.gene93060.t1 MTR_5g018850 31.527 203 118 6 571 768 1310 1496 1.24e-18 91.7
MS.gene93060.t1 MTR_1g080340 25.733 307 157 11 506 756 269 560 2.87e-18 89.7
MS.gene93060.t1 MTR_1g069570 26.996 263 121 11 506 713 430 676 6.51e-17 85.5
MS.gene93060.t1 MTR_5g098680 36.076 158 87 4 324 476 267 415 7.04e-17 85.5
MS.gene93060.t1 MTR_8g102580 35.443 158 88 4 324 476 261 409 3.46e-16 83.2
MS.gene93060.t1 MTR_3g114910 33.333 153 88 4 324 476 187 325 4.64e-13 72.4
MS.gene93060.t1 MTR_7g445810 35.673 171 84 8 293 455 114 266 8.57e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93060.t1 AT4G13460 62.687 469 168 5 301 767 186 649 0.0 623
MS.gene93060.t1 AT4G13460 62.687 469 168 5 301 767 186 649 0.0 623
MS.gene93060.t1 AT2G33290 58.714 482 189 5 287 767 178 650 0.0 609
MS.gene93060.t1 AT2G33290 58.714 482 189 5 287 767 178 650 0.0 609
MS.gene93060.t1 AT2G33290 58.714 482 189 5 287 767 178 650 0.0 609
MS.gene93060.t1 AT2G33290 58.714 482 189 5 287 767 178 650 0.0 609
MS.gene93060.t1 AT2G35160 39.520 458 233 10 301 754 310 727 1.75e-95 314
MS.gene93060.t1 AT2G22740 38.316 475 270 8 289 754 299 759 3.82e-95 314
MS.gene93060.t1 AT2G22740 38.316 475 270 8 289 754 299 759 3.82e-95 314
MS.gene93060.t1 AT2G35160 39.520 458 233 10 301 754 346 763 3.91e-95 314
MS.gene93060.t1 AT2G35160 39.520 458 233 10 301 754 346 763 3.91e-95 314
MS.gene93060.t1 AT2G35160 39.520 458 233 10 301 754 346 763 3.91e-95 314
MS.gene93060.t1 AT2G35160 39.520 458 233 10 301 754 346 763 3.91e-95 314
MS.gene93060.t1 AT5G04940 39.651 459 234 12 316 756 207 640 4.38e-94 308
MS.gene93060.t1 AT5G04940 39.651 459 234 12 316 756 207 640 4.38e-94 308
MS.gene93060.t1 AT1G73100 37.691 459 245 12 316 756 204 639 1.20e-92 304
MS.gene93060.t1 AT5G13960 36.739 460 261 14 316 755 11 460 5.60e-84 276
MS.gene93060.t1 AT5G13960 36.042 480 276 15 296 755 126 594 1.92e-83 278
MS.gene93060.t1 AT1G17770 33.261 460 267 15 316 756 223 661 1.44e-71 248
MS.gene93060.t1 AT2G24740 35.903 454 241 15 317 756 307 724 8.29e-69 241
MS.gene93060.t1 AT5G47150 43.827 162 82 4 315 476 172 324 7.14e-30 121
MS.gene93060.t1 AT5G47160 42.105 171 89 5 304 473 247 408 8.39e-27 114
MS.gene93060.t1 AT5G47160 42.105 171 89 5 304 473 247 408 8.39e-27 114
MS.gene93060.t1 AT5G47160 42.105 171 89 5 304 473 247 408 8.39e-27 114
MS.gene93060.t1 AT2G23740 28.179 291 158 11 503 759 1090 1363 4.58e-23 106
MS.gene93060.t1 AT2G23740 28.179 291 158 11 503 759 1090 1363 4.58e-23 106
MS.gene93060.t1 AT2G23740 28.179 291 158 11 503 759 1090 1363 4.58e-23 106
MS.gene93060.t1 AT2G23740 28.179 291 158 11 503 759 1083 1356 4.65e-23 106
MS.gene93060.t1 AT2G05900 37.640 178 103 6 361 538 26 195 2.18e-20 93.2
MS.gene93060.t1 AT2G23740 27.686 242 127 10 503 713 1090 1314 1.80e-17 88.2
MS.gene93060.t1 AT2G23740 27.686 242 127 10 503 713 1090 1314 1.80e-17 88.2
MS.gene93060.t1 AT1G57820 35.849 159 87 5 324 476 277 426 2.62e-16 83.6
MS.gene93060.t1 AT1G57820 35.849 159 87 5 324 476 277 426 2.62e-16 83.6
MS.gene93060.t1 AT3G03750 30.583 206 115 6 552 755 144 323 7.97e-16 80.5
MS.gene93060.t1 AT1G57820 35.256 156 89 5 324 476 277 423 2.34e-15 80.5
MS.gene93060.t1 AT1G66040 33.333 159 91 4 324 476 262 411 3.34e-14 77.0
MS.gene93060.t1 AT1G66050 33.333 159 91 4 324 476 262 411 3.71e-14 76.6
MS.gene93060.t1 AT5G39550 34.591 159 89 5 324 476 262 411 4.80e-14 76.3
MS.gene93060.t1 AT1G04050 24.595 309 155 10 506 756 409 697 5.30e-14 76.3
MS.gene93060.t1 AT1G04050 24.595 309 155 10 506 756 409 697 5.30e-14 76.3
MS.gene93060.t1 AT1G04050 24.595 309 155 10 506 756 305 593 5.78e-14 76.3
MS.gene93060.t1 AT4G08590 31.818 154 91 4 324 476 237 377 1.37e-11 68.2

Find 215 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGGAAGATTAAGAAAAT+TGG 0.178663 8.2:+32725395 MS.gene93060:CDS
CATCAAAATGATTAGGTTTA+GGG 0.226810 8.2:-32724089 None:intergenic
ACTAAATCATAACCTTAATA+TGG 0.228635 8.2:+32724362 MS.gene93060:CDS
TCATCAAAATGATTAGGTTT+AGG 0.248789 8.2:-32724090 None:intergenic
ACTCGTTAATATATCCAAAA+AGG 0.268007 8.2:+32725912 MS.gene93060:CDS
TGTTTCTGAAGTTGCGATTA+TGG 0.271621 8.2:+32724704 MS.gene93060:CDS
TGATTCTTGTTTATTTGAAA+TGG 0.272590 8.2:-32724427 None:intergenic
CAACAACCTCATTTCCCTTC+TGG 0.282693 8.2:+32724138 MS.gene93060:CDS
AAGCAAACCCTGAAGATTAT+AGG 0.291078 8.2:-32725657 None:intergenic
AATTATTGAGGAGAAGAAAT+TGG 0.298748 8.2:+32724611 MS.gene93060:CDS
AGAGTTTCCCTATAATCTTC+AGG 0.299770 8.2:+32725649 MS.gene93060:CDS
AGCCTTTATCTGCGAGTATA+CGG 0.307518 8.2:+32725841 MS.gene93060:CDS
GTTAATGATAAAGGATCTAT+TGG 0.313384 8.2:-32723943 None:intergenic
AGCTTGCCCGCCCACTCGTC+AGG 0.315436 8.2:-32726205 None:intergenic
AGTAGCTCTGGTTAAGAAAA+AGG 0.316965 8.2:+32724458 MS.gene93060:CDS
ATGTCATTAAATAGCCTAAT+AGG 0.327262 8.2:-32725479 None:intergenic
AGCAACACGATTCCGGCAAT+TGG 0.329682 8.2:-32725732 None:intergenic
AAGTGGTTATGAGGATGATA+TGG 0.333296 8.2:+32725049 MS.gene93060:CDS
AAGCGGTGCTCCGGTGGAAA+TGG 0.334829 8.2:-32724052 None:intergenic
GCTCTTGTCCTCCCAATTGC+CGG 0.336141 8.2:+32725720 MS.gene93060:CDS
GGATTGAGGGTTGACATGTT+AGG 0.336861 8.2:-32724018 None:intergenic
AGTGTAGAGGTTAGATTGAT+TGG 0.337421 8.2:-32724208 None:intergenic
ATACATTTGGACTCGAACTA+TGG 0.339033 8.2:-32726072 None:intergenic
CACAAACTGGTTGGGGAGTT+AGG 0.342051 8.2:+32725795 MS.gene93060:CDS
ATCATATTCGTGGTGACATA+TGG 0.343625 8.2:+32724814 MS.gene93060:CDS
TATGGTTGTATCGCGATAAA+AGG 0.345751 8.2:+32724865 MS.gene93060:CDS
GGTGGAAATGGGAGTGAGTT+GGG 0.348906 8.2:-32724040 None:intergenic
AAGAAATCATCAAAATGATT+AGG 0.351239 8.2:-32724096 None:intergenic
TGTATAGGATTATTGATTGC+TGG 0.360049 8.2:+32725279 MS.gene93060:CDS
CCGGAATCGTGTTGCTCAAA+AGG 0.365089 8.2:+32725739 MS.gene93060:CDS
AGCATAGGTTGGAAGTGTTT+AGG 0.365235 8.2:+32725768 MS.gene93060:CDS
GTTTCAAGGGTTAATGATAA+AGG 0.365643 8.2:-32723952 None:intergenic
ACCTGATATAGATTATTCTA+TGG 0.365689 8.2:+32726012 MS.gene93060:CDS
GCATTACGGGATCGAGATTA+GGG 0.365857 8.2:+32725190 MS.gene93060:CDS
CTAGGAAGATTAAGAAAATT+GGG 0.368395 8.2:+32725396 MS.gene93060:CDS
AGCAAACCCTGAAGATTATA+GGG 0.370075 8.2:-32725656 None:intergenic
TCCATAGAATAATCTATATC+AGG 0.373576 8.2:-32726013 None:intergenic
TGGGAGTGAGTTGGGGATTG+AGG 0.377704 8.2:-32724032 None:intergenic
ATTAGGTTTAGGGACGAGTA+TGG 0.379859 8.2:-32724079 None:intergenic
GATTGAGGGTTGACATGTTA+GGG 0.381292 8.2:-32724017 None:intergenic
ATGGTGGAATTATGTGTAAT+TGG 0.382160 8.2:+32724936 MS.gene93060:CDS
TTGGGAGGACAAGAGCAAAA+AGG 0.382424 8.2:-32725713 None:intergenic
TATAGATAGATGGGCAGAAT+GGG 0.382516 8.2:+32725937 MS.gene93060:CDS
CATCAGAGCGGGAAAGCTAT+TGG 0.382523 8.2:+32725569 MS.gene93060:CDS
TAATCTATATCAGGTATTAA+TGG 0.385147 8.2:-32726004 None:intergenic
TCCCGTATACTCGCAGATAA+AGG 0.385176 8.2:-32725843 None:intergenic
TAGGTCTGCACAAACTGGTT+GGG 0.389899 8.2:+32725787 MS.gene93060:CDS
TGCATTACGGGATCGAGATT+AGG 0.391240 8.2:+32725189 MS.gene93060:CDS
AGAGGGAGAATGTTCCTATT+AGG 0.395044 8.2:+32725465 MS.gene93060:CDS
TGAGAAGGGGAGGTAATTGA+AGG 0.395212 8.2:-32723976 None:intergenic
AATTCTTCTTTCTTCCTCCT+TGG 0.396457 8.2:-32725106 None:intergenic
ACAAACTGAACAAATACATT+TGG 0.398498 8.2:-32726085 None:intergenic
CTAAATCATAACCTTAATAT+GGG 0.399163 8.2:+32724363 MS.gene93060:CDS
TAAAGGCTCCAACTTGAATA+AGG 0.399412 8.2:-32725826 None:intergenic
AGGTTGTTGTTGGGAACCTT+GGG 0.401571 8.2:-32724124 None:intergenic
GATTCTTGTTTATTTGAAAT+GGG 0.406495 8.2:-32724426 None:intergenic
TAAAGGATCTATTGGATTCA+TGG 0.409301 8.2:-32723935 None:intergenic
TATAAGTTATTGAGAGTTGA+AGG 0.409965 8.2:+32725338 MS.gene93060:CDS
GGTAAGGTTTATGTTTATGA+TGG 0.410667 8.2:+32725254 MS.gene93060:CDS
CTGATCACAAATTTGAATTG+AGG 0.414878 8.2:-32725129 None:intergenic
AGAGATTGGTTGAGGAGAAG+AGG 0.415274 8.2:+32724661 MS.gene93060:CDS
AAACTCTCCTCCATTCTTCA+TGG 0.416886 8.2:-32725633 None:intergenic
ATTGGTTGAGGAGAAGAGGT+TGG 0.418514 8.2:+32724665 MS.gene93060:CDS
GGGAGTGAGTTGGGGATTGA+GGG 0.418786 8.2:-32724031 None:intergenic
GGAAGATACTCATAATTAAG+AGG 0.420348 8.2:-32725518 None:intergenic
ATGTTTATGATGGATTGTAT+AGG 0.423249 8.2:+32725264 MS.gene93060:CDS
TTATCGAAAGTAGCATTAGA+AGG 0.423817 8.2:-32724252 None:intergenic
CAATGCTTATGCCGCTTCAA+TGG 0.427312 8.2:+32724311 MS.gene93060:CDS
TGTGGAGGCAGATAAGAGAT+TGG 0.428163 8.2:+32724647 MS.gene93060:CDS
AGAGAACTCGTCATAGACTA+TGG 0.430962 8.2:+32726178 MS.gene93060:CDS
GGGTTGACATGTTAGGGATT+TGG 0.431533 8.2:-32724011 None:intergenic
GGTGAGAAGAAGTTTGAGAA+GGG 0.432588 8.2:-32723990 None:intergenic
AGAAGAGGTTGGATGAGGAT+AGG 0.436158 8.2:+32724676 MS.gene93060:CDS
CGGAATCGTGTTGCTCAAAA+GGG 0.437967 8.2:+32725740 MS.gene93060:CDS
TCAATAGAAAGACAATTCAT+AGG 0.440117 8.2:-32725431 None:intergenic
TGAAGCGGCATAAGCATTGT+TGG 0.444430 8.2:-32724307 None:intergenic
AGTAGCAATTGGCTCGCCAT+TGG 0.452219 8.2:-32725012 None:intergenic
GTGTTTAGGTCTGCACAAAC+TGG 0.452666 8.2:+32725782 MS.gene93060:CDS
AAGGGAAATGAGGTTGTTGT+TGG 0.453858 8.2:-32724134 None:intergenic
AGGATAGTTGGTTCGATACC+TGG 0.455737 8.2:+32724885 MS.gene93060:CDS
TTATGAGGATGATATGGAAT+TGG 0.457955 8.2:+32725055 MS.gene93060:CDS
GGCAGATAAGAGATTGGTTG+AGG 0.458910 8.2:+32724653 MS.gene93060:CDS
GATGATATGGAATTGGATAA+TGG 0.459588 8.2:+32725062 MS.gene93060:CDS
TCCTTGGACCTTATTCAAGT+TGG 0.460907 8.2:+32725818 MS.gene93060:CDS
GAGTTTCCCTATAATCTTCA+GGG 0.462595 8.2:+32725650 MS.gene93060:CDS
AGGTAATTGAAGGGGTTTCA+AGG 0.463004 8.2:-32723966 None:intergenic
CTTCTTTCTTCCTCCTTGGC+CGG 0.463064 8.2:-32725102 None:intergenic
ATTGATTGCTGGTTTGAAGA+AGG 0.466360 8.2:+32725290 MS.gene93060:CDS
AATCAATATCATTTCCCTCC+TGG 0.466834 8.2:+32724174 MS.gene93060:CDS
GTAGCAATTGGCTCGCCATT+GGG 0.469298 8.2:-32725011 None:intergenic
ATCATGTTCGGCAGTAGCTC+TGG 0.471165 8.2:+32724446 MS.gene93060:CDS
ACAATCACACTAGTAGCAAT+TGG 0.471909 8.2:-32725023 None:intergenic
AGTTGAAGGGCAAACTAAGA+TGG 0.474128 8.2:+32725352 MS.gene93060:CDS
TATCGCGATAAAAGGATAGT+TGG 0.479508 8.2:+32724873 MS.gene93060:CDS
TTAGAGCGTAGTATGCATTA+CGG 0.480635 8.2:+32725176 MS.gene93060:CDS
TAAGGTTATGATTTAGTCCA+AGG 0.481584 8.2:-32724356 None:intergenic
TCAGTCAATATTTCTGACCT+TGG 0.482765 8.2:+32724339 MS.gene93060:CDS
GATTGTTTCAAGTGGTTATG+AGG 0.483946 8.2:+32725040 MS.gene93060:CDS
TAGAGCGTAGTATGCATTAC+GGG 0.486228 8.2:+32725177 MS.gene93060:CDS
GAAGATTAAGAAAATTGGGT+TGG 0.487319 8.2:+32725400 MS.gene93060:CDS
TCAGGTATTAATGGATATGA+TGG 0.489556 8.2:-32725995 None:intergenic
TAATGAAGGACCGAGAGTTA+TGG 0.490732 8.2:+32724847 MS.gene93060:CDS
GCAACACGATTCCGGCAATT+GGG 0.491457 8.2:-32725731 None:intergenic
AGCGGTGCTCCGGTGGAAAT+GGG 0.492091 8.2:-32724051 None:intergenic
AGGGAAATGAGGTTGTTGTT+GGG 0.492139 8.2:-32724133 None:intergenic
AGACGTTATAATCATATTCG+TGG 0.492546 8.2:+32724804 MS.gene93060:CDS
GGATGAGAAAAGAATTATTG+AGG 0.492648 8.2:+32724599 MS.gene93060:CDS
GCCTTTATCTGCGAGTATAC+GGG 0.492679 8.2:+32725842 MS.gene93060:CDS
TTGATTGCTGGTTTGAAGAA+GGG 0.494752 8.2:+32725291 MS.gene93060:CDS
CTTCAGGGTTTGCTTGTGAA+AGG 0.494789 8.2:+32725665 MS.gene93060:CDS
TCATATTCGTGGTGACATAT+GGG 0.495464 8.2:+32724815 MS.gene93060:CDS
GCGATTATGGAGACGGAGAT+GGG 0.496444 8.2:+32724717 MS.gene93060:CDS
TGATTGGTGTGTTCATATCC+AGG 0.498306 8.2:-32724192 None:intergenic
TGCGATTATGGAGACGGAGA+TGG 0.502769 8.2:+32724716 MS.gene93060:CDS
TATGGTTTGAAGCGGTGCTC+CGG 0.502918 8.2:-32724061 None:intergenic
TTGTTGTTGGGAACCTTGGG+TGG 0.503224 8.2:-32724121 None:intergenic
TGTGTGGAGGCTTGTACTGA+TGG 0.507598 8.2:+32725599 MS.gene93060:CDS
TGAGGAGAAGAGGTTGGATG+AGG 0.507616 8.2:+32724671 MS.gene93060:CDS
AGGTCTGCACAAACTGGTTG+GGG 0.507788 8.2:+32725788 MS.gene93060:CDS
GTGAGAAGAAGTTTGAGAAG+GGG 0.507936 8.2:-32723989 None:intergenic
AAATAAACAAGAATCATGTT+CGG 0.510967 8.2:+32724434 MS.gene93060:CDS
TAGATAGATGGGCAGAATGG+GGG 0.511424 8.2:+32725939 MS.gene93060:CDS
ACATCAGATTGTTATGATCA+TGG 0.511816 8.2:-32726111 None:intergenic
GAGGTTGTTGTTGGGAACCT+TGG 0.515342 8.2:-32724125 None:intergenic
AGAAGAAGTTTGAGAAGGGG+AGG 0.515562 8.2:-32723986 None:intergenic
TGGTGAGAAGAAGTTTGAGA+AGG 0.516679 8.2:-32723991 None:intergenic
TTATAGATAGATGGGCAGAA+TGG 0.518603 8.2:+32725936 MS.gene93060:CDS
ATGGAGACGGAGATGGGAGC+TGG 0.518912 8.2:+32724723 MS.gene93060:CDS
GGCATCCCTGACGAGTGGGC+GGG 0.520972 8.2:+32726199 MS.gene93060:CDS
TGAAGTTGCGATTATGGAGA+CGG 0.522248 8.2:+32724710 MS.gene93060:CDS
GATGATTTCTTCACCACCCA+AGG 0.524018 8.2:+32724108 MS.gene93060:CDS
CGGTGGAAATGGGAGTGAGT+TGG 0.525640 8.2:-32724041 None:intergenic
AGATAGATGGGCAGAATGGG+GGG 0.535500 8.2:+32725940 MS.gene93060:CDS
TTCGTGGGATGAAGTATGAA+GGG 0.537787 8.2:+32725216 MS.gene93060:CDS
TGGTTTGAAGAAGGGATGAA+TGG 0.538003 8.2:+32725299 MS.gene93060:CDS
GGTGATGTAATAATCTATAC+CGG 0.538669 8.2:+32725083 MS.gene93060:CDS
AATTTGTGATCAGAAATTAG+AGG 0.546612 8.2:+32725139 MS.gene93060:CDS
GACTGAGTTCTCCATTGAAG+CGG 0.547564 8.2:-32724322 None:intergenic
AAAGGGGATCAAGCATAGGT+TGG 0.547811 8.2:+32725757 MS.gene93060:CDS
TCGATACCTGGAGTTTATGT+TGG 0.551588 8.2:+32724897 MS.gene93060:CDS
ATAAGTTATTGAGAGTTGAA+GGG 0.555115 8.2:+32725339 MS.gene93060:CDS
AAAGTAGCATTAGAAGGTTG+TGG 0.556976 8.2:-32724246 None:intergenic
GGTTTGTATTTCATCAGAGC+GGG 0.557067 8.2:+32725558 MS.gene93060:CDS
CAAATATGAGTATGAAGGAT+CGG 0.557193 8.2:+32724505 MS.gene93060:CDS
ATGGAGGGCGAAATGTTGAA+TGG 0.558277 8.2:+32724777 MS.gene93060:CDS
ATCGAGATTAGGGTTATTCG+TGG 0.558682 8.2:+32725200 MS.gene93060:CDS
AGAGGCAGCAACATGGATGG+AGG 0.559470 8.2:+32724761 MS.gene93060:CDS
CCGTCTCATGCTATATGCAA+TGG 0.560658 8.2:+32726138 MS.gene93060:CDS
CGCGATACAACCATAACTCT+CGG 0.563453 8.2:-32724857 None:intergenic
TATTGATATTTCACACAAGA+GGG 0.564342 8.2:+32725448 MS.gene93060:CDS
TTAGGTCTGCACAAACTGGT+TGG 0.564740 8.2:+32725786 MS.gene93060:CDS
TGGTGATGAGAACATGGAAG+AGG 0.565897 8.2:+32724743 MS.gene93060:CDS
GGGTGCGTCTAGCTCTGGTA+AGG 0.568302 8.2:+32725238 MS.gene93060:CDS
GTGGAAATGGGAGTGAGTTG+GGG 0.568955 8.2:-32724039 None:intergenic
TGGCATCCCTGACGAGTGGG+CGG 0.569853 8.2:+32726198 MS.gene93060:CDS
ATTCGTGGGATGAAGTATGA+AGG 0.571493 8.2:+32725215 MS.gene93060:CDS
CTATTGATATTTCACACAAG+AGG 0.573542 8.2:+32725447 MS.gene93060:CDS
TGTTGTAGTACTTAGTGTAG+AGG 0.573712 8.2:-32724221 None:intergenic
CATTGCATATAGCATGAGAC+GGG 0.574005 8.2:-32726137 None:intergenic
ATAGATAGATGGGCAGAATG+GGG 0.576328 8.2:+32725938 MS.gene93060:CDS
GGTTTGAAGCGGTGCTCCGG+TGG 0.577375 8.2:-32724058 None:intergenic
GTGTTGTTCTCAAAGATGCT+AGG 0.577721 8.2:+32725378 MS.gene93060:CDS
ATTTGTGATCAGAAATTAGA+GGG 0.579266 8.2:+32725140 MS.gene93060:CDS
ATGGGCTGCGAGGTTAATGA+AGG 0.580888 8.2:+32724833 MS.gene93060:CDS
GGAAGAGGCAGCAACATGGA+TGG 0.581307 8.2:+32724758 MS.gene93060:CDS
GCTTGCCCGCCCACTCGTCA+GGG 0.582498 8.2:-32726204 None:intergenic
TTGCATATGCAGATACAAGC+TGG 0.583868 8.2:+32724960 MS.gene93060:CDS
CAACTCACTCCCATTTCCAC+CGG 0.584867 8.2:+32724042 MS.gene93060:CDS
GCAACATGATGAGGAGAACA+AGG 0.586300 8.2:+32724538 MS.gene93060:CDS
GTAATAATCTATACCGGCCA+AGG 0.586629 8.2:+32725089 MS.gene93060:CDS
CCATTGCATATAGCATGAGA+CGG 0.588288 8.2:-32726138 None:intergenic
AGAAGGGGAGGTAATTGAAG+GGG 0.591585 8.2:-32723974 None:intergenic
GAGAAGGGGAGGTAATTGAA+GGG 0.591667 8.2:-32723975 None:intergenic
GTATTCAAATATGAGTATGA+AGG 0.592101 8.2:+32724500 MS.gene93060:CDS
GGTAATTGAAGGGGTTTCAA+GGG 0.592733 8.2:-32723965 None:intergenic
AGCTATTGGCTGTGAATGTG+TGG 0.596741 8.2:+32725583 MS.gene93060:CDS
CTCTGATGAAATACAAACCA+CGG 0.598765 8.2:-32725554 None:intergenic
ACATCACCAACATAAACTCC+AGG 0.599500 8.2:-32724903 None:intergenic
TGGTTGGGGAGTTAGGTCCT+TGG 0.600362 8.2:+32725802 MS.gene93060:CDS
TTGTTGTGTTCATATCCAGA+AGG 0.602561 8.2:-32724153 None:intergenic
GGGAGCTGGTGATGAGAACA+TGG 0.604987 8.2:+32724737 MS.gene93060:CDS
GTTGAAGGGCAAACTAAGAT+GGG 0.605405 8.2:+32725353 MS.gene93060:CDS
GCATACTACGCTCTAAAGCA+AGG 0.606571 8.2:-32725169 None:intergenic
GGGACGAGTATGGTTTGAAG+CGG 0.607757 8.2:-32724069 None:intergenic
TCTATGACGAGTTCTCTCAA+TGG 0.607917 8.2:-32726172 None:intergenic
ATGACGAGTTCTCTCAATGG+AGG 0.609086 8.2:-32726169 None:intergenic
TAAATCATAACCTTAATATG+GGG 0.609176 8.2:+32724364 MS.gene93060:CDS
TCATATCCAGAAGGGAAATG+AGG 0.611409 8.2:-32724144 None:intergenic
TGTTGTGTTCATATCCAGAA+GGG 0.611765 8.2:-32724152 None:intergenic
ACTAGTGTGATTGTTTCAAG+TGG 0.613031 8.2:+32725032 MS.gene93060:CDS
ACGTGTGTTAGCAAGTATTG+AGG 0.613466 8.2:+32724578 MS.gene93060:CDS
AGGCAAGTCAGATGCTTGTG+AGG 0.614350 8.2:+32724478 MS.gene93060:CDS
TCGTGGGATGAAGTATGAAG+GGG 0.618537 8.2:+32725217 MS.gene93060:CDS
CTATGGCATCCCTGACGAGT+GGG 0.626017 8.2:+32726195 MS.gene93060:CDS
TCCAACTTGAATAAGGTCCA+AGG 0.627230 8.2:-32725819 None:intergenic
GAGGCAGCAACATGGATGGA+GGG 0.628691 8.2:+32724762 MS.gene93060:CDS
ACATGGAAGAGGCAGCAACA+TGG 0.634502 8.2:+32724754 MS.gene93060:CDS
TCGAGATTAGGGTTATTCGT+GGG 0.635248 8.2:+32725201 MS.gene93060:CDS
CTCAAAAGGGGATCAAGCAT+AGG 0.636242 8.2:+32725753 MS.gene93060:CDS
ACACGATTCCGGCAATTGGG+AGG 0.637681 8.2:-32725728 None:intergenic
TGTGATCAGAAATTAGAGGG+TGG 0.640509 8.2:+32725143 MS.gene93060:CDS
TGACGAGTTCTCTCAATGGA+GGG 0.642236 8.2:-32726168 None:intergenic
AAATCATAACCTTAATATGG+GGG 0.649898 8.2:+32724365 MS.gene93060:CDS
ACTATGGCATCCCTGACGAG+TGG 0.651275 8.2:+32726194 MS.gene93060:CDS
GGAATCGTGTTGCTCAAAAG+GGG 0.665750 8.2:+32725741 MS.gene93060:CDS
TGGTGTGTTCATATCCAGGA+GGG 0.666587 8.2:-32724188 None:intergenic
TGGTTTGTATTTCATCAGAG+CGG 0.676509 8.2:+32725557 MS.gene93060:CDS
TATTGGCTGTGAATGTGTGG+AGG 0.683730 8.2:+32725586 MS.gene93060:CDS
CCTAAAAGTAGGTCTCCCAA+TGG 0.692154 8.2:+32724996 MS.gene93060:CDS
CGTGGGATGAAGTATGAAGG+GGG 0.704890 8.2:+32725218 MS.gene93060:CDS
ATAATCTATACCGGCCAAGG+AGG 0.705547 8.2:+32725092 MS.gene93060:CDS
GTGGTGACATATGGGCTGCG+AGG 0.720019 8.2:+32724823 MS.gene93060:CDS
TTGGTGTGTTCATATCCAGG+AGG 0.720395 8.2:-32724189 None:intergenic
ATTGCATATAGCATGAGACG+GGG 0.721884 8.2:-32726136 None:intergenic
TTGCATATAGCATGAGACGG+GGG 0.722787 8.2:-32726135 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGGTTTATAGATAGAT+GGG + chr8.2:32725928-32725947 MS.gene93060:CDS 20.0%
!! AAATAAACAAGAATCATGTT+CGG + chr8.2:32724434-32724453 MS.gene93060:CDS 20.0%
!! AAGAAATCATCAAAATGATT+AGG - chr8.2:32724099-32724118 None:intergenic 20.0%
!! ACTAAATCATAACCTTAATA+TGG + chr8.2:32724362-32724381 MS.gene93060:CDS 20.0%
!! CTAAATCATAACCTTAATAT+GGG + chr8.2:32724363-32724382 MS.gene93060:CDS 20.0%
!! TAAATCATAACCTTAATATG+GGG + chr8.2:32724364-32724383 MS.gene93060:CDS 20.0%
!! TAATCTATATCAGGTATTAA+TGG - chr8.2:32726007-32726026 None:intergenic 20.0%
!!! GATTCTTGTTTATTTGAAAT+GGG - chr8.2:32724429-32724448 None:intergenic 20.0%
!!! GATTTTGAGAAAATGATAAA+GGG - chr8.2:32724280-32724299 None:intergenic 20.0%
!!! TGATTCTTGTTTATTTGAAA+TGG - chr8.2:32724430-32724449 None:intergenic 20.0%
! AAATCATAACCTTAATATGG+GGG + chr8.2:32724365-32724384 MS.gene93060:CDS 25.0%
! AATTATTGAGGAGAAGAAAT+TGG + chr8.2:32724611-32724630 MS.gene93060:CDS 25.0%
! AATTTGTGATCAGAAATTAG+AGG + chr8.2:32725139-32725158 MS.gene93060:CDS 25.0%
! ACAAACTGAACAAATACATT+TGG - chr8.2:32726088-32726107 None:intergenic 25.0%
! ACCTGATATAGATTATTCTA+TGG + chr8.2:32726012-32726031 MS.gene93060:CDS 25.0%
! ACTCGTTAATATATCCAAAA+AGG + chr8.2:32725912-32725931 MS.gene93060:CDS 25.0%
! ATAAGTTATTGAGAGTTGAA+GGG + chr8.2:32725339-32725358 MS.gene93060:CDS 25.0%
! ATGTCATTAAATAGCCTAAT+AGG - chr8.2:32725482-32725501 None:intergenic 25.0%
! ATTTGTGATCAGAAATTAGA+GGG + chr8.2:32725140-32725159 MS.gene93060:CDS 25.0%
! CAAAAAGGTTTATAGATAGA+TGG + chr8.2:32725927-32725946 MS.gene93060:CDS 25.0%
! CATCAAAATGATTAGGTTTA+GGG - chr8.2:32724092-32724111 None:intergenic 25.0%
! CTAGGAAGATTAAGAAAATT+GGG + chr8.2:32725396-32725415 MS.gene93060:CDS 25.0%
! GTATTCAAATATGAGTATGA+AGG + chr8.2:32724500-32724519 MS.gene93060:CDS 25.0%
! GTTAATGATAAAGGATCTAT+TGG - chr8.2:32723946-32723965 None:intergenic 25.0%
! TATAAGTTATTGAGAGTTGA+AGG + chr8.2:32725338-32725357 MS.gene93060:CDS 25.0%
! TATTGATATTTCACACAAGA+GGG + chr8.2:32725448-32725467 MS.gene93060:CDS 25.0%
! TCAATAGAAAGACAATTCAT+AGG - chr8.2:32725434-32725453 None:intergenic 25.0%
! TCATCAAAATGATTAGGTTT+AGG - chr8.2:32724093-32724112 None:intergenic 25.0%
! TCCATAGAATAATCTATATC+AGG - chr8.2:32726016-32726035 None:intergenic 25.0%
!! CATCTATCTATAAACCTTTT+TGG - chr8.2:32725929-32725948 None:intergenic 25.0%
!! TTGATTTTCTTCCTAAAAGT+AGG + chr8.2:32724985-32725004 MS.gene93060:CDS 25.0%
!!! ATGTTTATGATGGATTGTAT+AGG + chr8.2:32725264-32725283 MS.gene93060:CDS 25.0%
!!! CGATTTTGAGAAAATGATAA+AGG - chr8.2:32724281-32724300 None:intergenic 25.0%
!!! GCTCAAATTTTAACAATGAA+TGG + chr8.2:32725887-32725906 MS.gene93060:CDS 25.0%
ACATCAGATTGTTATGATCA+TGG - chr8.2:32726114-32726133 None:intergenic 30.0%
AGACGTTATAATCATATTCG+TGG + chr8.2:32724804-32724823 MS.gene93060:CDS 30.0%
CAAATATGAGTATGAAGGAT+CGG + chr8.2:32724505-32724524 MS.gene93060:CDS 30.0%
CTATTGATATTTCACACAAG+AGG + chr8.2:32725447-32725466 MS.gene93060:CDS 30.0%
CTGATCACAAATTTGAATTG+AGG - chr8.2:32725132-32725151 None:intergenic 30.0%
GAAGATTAAGAAAATTGGGT+TGG + chr8.2:32725400-32725419 MS.gene93060:CDS 30.0%
GATGATATGGAATTGGATAA+TGG + chr8.2:32725062-32725081 MS.gene93060:CDS 30.0%
GCTAGGAAGATTAAGAAAAT+TGG + chr8.2:32725395-32725414 MS.gene93060:CDS 30.0%
GGAAGATACTCATAATTAAG+AGG - chr8.2:32725521-32725540 None:intergenic 30.0%
GGATGAGAAAAGAATTATTG+AGG + chr8.2:32724599-32724618 MS.gene93060:CDS 30.0%
GGTAAGGTTTATGTTTATGA+TGG + chr8.2:32725254-32725273 MS.gene93060:CDS 30.0%
GGTGATGTAATAATCTATAC+CGG + chr8.2:32725083-32725102 MS.gene93060:CDS 30.0%
GTTTCAAGGGTTAATGATAA+AGG - chr8.2:32723955-32723974 None:intergenic 30.0%
TAAGGTTATGATTTAGTCCA+AGG - chr8.2:32724359-32724378 None:intergenic 30.0%
TTATCGAAAGTAGCATTAGA+AGG - chr8.2:32724255-32724274 None:intergenic 30.0%
TTATGAGGATGATATGGAAT+TGG + chr8.2:32725055-32725074 MS.gene93060:CDS 30.0%
! AACCACGGAAAAATAGTTTT+TGG - chr8.2:32725542-32725561 None:intergenic 30.0%
! ATGGTGGAATTATGTGTAAT+TGG + chr8.2:32724936-32724955 MS.gene93060:CDS 30.0%
! TCAGGTATTAATGGATATGA+TGG - chr8.2:32725998-32726017 None:intergenic 30.0%
! TGATGTTTTCTTGTATATGG+TGG + chr8.2:32724920-32724939 MS.gene93060:CDS 30.0%
! TGTATAGGATTATTGATTGC+TGG + chr8.2:32725279-32725298 MS.gene93060:CDS 30.0%
!! AAGCCTTTGATTTTTGAATG+TGG + chr8.2:32725689-32725708 MS.gene93060:CDS 30.0%
!! ATACGGGAGTAATTTTAACA+AGG + chr8.2:32725858-32725877 MS.gene93060:CDS 30.0%
!! TACGGGAGTAATTTTAACAA+GGG + chr8.2:32725859-32725878 MS.gene93060:CDS 30.0%
!! TGGTGATGTTTTCTTGTATA+TGG + chr8.2:32724917-32724936 MS.gene93060:CDS 30.0%
!! TTCCAAAAACTATTTTTCCG+TGG + chr8.2:32725537-32725556 MS.gene93060:CDS 30.0%
AAAGTAGCATTAGAAGGTTG+TGG - chr8.2:32724249-32724268 None:intergenic 35.0%
AAGCAAACCCTGAAGATTAT+AGG - chr8.2:32725660-32725679 None:intergenic 35.0%
AAGTGGTTATGAGGATGATA+TGG + chr8.2:32725049-32725068 MS.gene93060:CDS 35.0%
AATCAATATCATTTCCCTCC+TGG + chr8.2:32724174-32724193 MS.gene93060:CDS 35.0%
AATTCTTCTTTCTTCCTCCT+TGG - chr8.2:32725109-32725128 None:intergenic 35.0%
ACAATCACACTAGTAGCAAT+TGG - chr8.2:32725026-32725045 None:intergenic 35.0%
ACTAGTGTGATTGTTTCAAG+TGG + chr8.2:32725032-32725051 MS.gene93060:CDS 35.0%
AGAGTTTCCCTATAATCTTC+AGG + chr8.2:32725649-32725668 MS.gene93060:CDS 35.0%
AGCAAACCCTGAAGATTATA+GGG - chr8.2:32725659-32725678 None:intergenic 35.0%
AGTGTAGAGGTTAGATTGAT+TGG - chr8.2:32724211-32724230 None:intergenic 35.0%
ATACATTTGGACTCGAACTA+TGG - chr8.2:32726075-32726094 None:intergenic 35.0%
ATCATATTCGTGGTGACATA+TGG + chr8.2:32724814-32724833 MS.gene93060:CDS 35.0%
CTCTGATGAAATACAAACCA+CGG - chr8.2:32725557-32725576 None:intergenic 35.0%
GAGTTTCCCTATAATCTTCA+GGG + chr8.2:32725650-32725669 MS.gene93060:CDS 35.0%
TAAAGGCTCCAACTTGAATA+AGG - chr8.2:32725829-32725848 None:intergenic 35.0%
TATAGATAGATGGGCAGAAT+GGG + chr8.2:32725937-32725956 MS.gene93060:CDS 35.0%
TATCGCGATAAAAGGATAGT+TGG + chr8.2:32724873-32724892 MS.gene93060:CDS 35.0%
TATGGTTGTATCGCGATAAA+AGG + chr8.2:32724865-32724884 MS.gene93060:CDS 35.0%
TCATATTCGTGGTGACATAT+GGG + chr8.2:32724815-32724834 MS.gene93060:CDS 35.0%
TGTTGTAGTACTTAGTGTAG+AGG - chr8.2:32724224-32724243 None:intergenic 35.0%
TGTTGTGTTCATATCCAGAA+GGG - chr8.2:32724155-32724174 None:intergenic 35.0%
TTAGAGCGTAGTATGCATTA+CGG + chr8.2:32725176-32725195 MS.gene93060:CDS 35.0%
TTATAGATAGATGGGCAGAA+TGG + chr8.2:32725936-32725955 MS.gene93060:CDS 35.0%
TTGTTGTGTTCATATCCAGA+AGG - chr8.2:32724156-32724175 None:intergenic 35.0%
! GATTGTTTCAAGTGGTTATG+AGG + chr8.2:32725040-32725059 MS.gene93060:CDS 35.0%
! TCAGTCAATATTTCTGACCT+TGG + chr8.2:32724339-32724358 MS.gene93060:CDS 35.0%
! TGCATTTTTGCAACATGATG+AGG + chr8.2:32724529-32724548 MS.gene93060:CDS 35.0%
! TGGTTTGTATTTCATCAGAG+CGG + chr8.2:32725557-32725576 MS.gene93060:CDS 35.0%
!! AGTAGCTCTGGTTAAGAAAA+AGG + chr8.2:32724458-32724477 MS.gene93060:CDS 35.0%
!! ATTGATTGCTGGTTTGAAGA+AGG + chr8.2:32725290-32725309 MS.gene93060:CDS 35.0%
!! TGTTTCTGAAGTTGCGATTA+TGG + chr8.2:32724704-32724723 MS.gene93060:CDS 35.0%
!! TTGATTGCTGGTTTGAAGAA+GGG + chr8.2:32725291-32725310 MS.gene93060:CDS 35.0%
AAACTCTCCTCCATTCTTCA+TGG - chr8.2:32725636-32725655 None:intergenic 40.0%
AAGGGAAATGAGGTTGTTGT+TGG - chr8.2:32724137-32724156 None:intergenic 40.0%
ACATCACCAACATAAACTCC+AGG - chr8.2:32724906-32724925 None:intergenic 40.0%
AGAGAACTCGTCATAGACTA+TGG + chr8.2:32726178-32726197 MS.gene93060:CDS 40.0%
AGAGGGAGAATGTTCCTATT+AGG + chr8.2:32725465-32725484 MS.gene93060:CDS 40.0%
AGCCTTTATCTGCGAGTATA+CGG + chr8.2:32725841-32725860 MS.gene93060:CDS 40.0%
AGGTAATTGAAGGGGTTTCA+AGG - chr8.2:32723969-32723988 None:intergenic 40.0%
AGTTGAAGGGCAAACTAAGA+TGG + chr8.2:32725352-32725371 MS.gene93060:CDS 40.0%
ATAGATAGATGGGCAGAATG+GGG + chr8.2:32725938-32725957 MS.gene93060:CDS 40.0%
ATCGAGATTAGGGTTATTCG+TGG + chr8.2:32725200-32725219 MS.gene93060:CDS 40.0%
ATTAGGTTTAGGGACGAGTA+TGG - chr8.2:32724082-32724101 None:intergenic 40.0%
ATTCGTGGGATGAAGTATGA+AGG + chr8.2:32725215-32725234 MS.gene93060:CDS 40.0%
ATTGCATATAGCATGAGACG+GGG - chr8.2:32726139-32726158 None:intergenic 40.0%
CAAAATCCACCCCCATATTA+AGG - chr8.2:32724377-32724396 None:intergenic 40.0%
CATTGCATATAGCATGAGAC+GGG - chr8.2:32726140-32726159 None:intergenic 40.0%
CCATTGCATATAGCATGAGA+CGG - chr8.2:32726141-32726160 None:intergenic 40.0%
GATTGAGGGTTGACATGTTA+GGG - chr8.2:32724020-32724039 None:intergenic 40.0%
GGGCCACATTCAAAAATCAA+AGG - chr8.2:32725695-32725714 None:intergenic 40.0%
GGTAATTGAAGGGGTTTCAA+GGG - chr8.2:32723968-32723987 None:intergenic 40.0%
GGTGAGAAGAAGTTTGAGAA+GGG - chr8.2:32723993-32724012 None:intergenic 40.0%
GGTTTGTATTTCATCAGAGC+GGG + chr8.2:32725558-32725577 MS.gene93060:CDS 40.0%
GTAATAATCTATACCGGCCA+AGG + chr8.2:32725089-32725108 MS.gene93060:CDS 40.0%
GTGAGAAGAAGTTTGAGAAG+GGG - chr8.2:32723992-32724011 None:intergenic 40.0%
GTTGAAGGGCAAACTAAGAT+GGG + chr8.2:32725353-32725372 MS.gene93060:CDS 40.0%
TAATGAAGGACCGAGAGTTA+TGG + chr8.2:32724847-32724866 MS.gene93060:CDS 40.0%
TAGAGCGTAGTATGCATTAC+GGG + chr8.2:32725177-32725196 MS.gene93060:CDS 40.0%
TCATAACCTTAATATGGGGG+TGG + chr8.2:32724368-32724387 MS.gene93060:CDS 40.0%
TCATATCCAGAAGGGAAATG+AGG - chr8.2:32724147-32724166 None:intergenic 40.0%
TCCAACTTGAATAAGGTCCA+AGG - chr8.2:32725822-32725841 None:intergenic 40.0%
TCCTTGGACCTTATTCAAGT+TGG + chr8.2:32725818-32725837 MS.gene93060:CDS 40.0%
TCGAGATTAGGGTTATTCGT+GGG + chr8.2:32725201-32725220 MS.gene93060:CDS 40.0%
TCGATACCTGGAGTTTATGT+TGG + chr8.2:32724897-32724916 MS.gene93060:CDS 40.0%
TCTATGACGAGTTCTCTCAA+TGG - chr8.2:32726175-32726194 None:intergenic 40.0%
TGAAGTTGCGATTATGGAGA+CGG + chr8.2:32724710-32724729 MS.gene93060:CDS 40.0%
TGATTGGTGTGTTCATATCC+AGG - chr8.2:32724195-32724214 None:intergenic 40.0%
TGGTGAGAAGAAGTTTGAGA+AGG - chr8.2:32723994-32724013 None:intergenic 40.0%
TGGTTTGAAGAAGGGATGAA+TGG + chr8.2:32725299-32725318 MS.gene93060:CDS 40.0%
TGTGATCAGAAATTAGAGGG+TGG + chr8.2:32725143-32725162 MS.gene93060:CDS 40.0%
TTCGTGGGATGAAGTATGAA+GGG + chr8.2:32725216-32725235 MS.gene93060:CDS 40.0%
TTGCATATGCAGATACAAGC+TGG + chr8.2:32724960-32724979 MS.gene93060:CDS 40.0%
! ACGTGTGTTAGCAAGTATTG+AGG + chr8.2:32724578-32724597 MS.gene93060:CDS 40.0%
! AGGGAAATGAGGTTGTTGTT+GGG - chr8.2:32724136-32724155 None:intergenic 40.0%
! ATTGGCTGACGAAGTTTTTG+TGG + chr8.2:32724629-32724648 MS.gene93060:CDS 40.0%
! GTGTTGTTCTCAAAGATGCT+AGG + chr8.2:32725378-32725397 MS.gene93060:CDS 40.0%
! TGCTTTTGCTCCATGAAGAA+TGG + chr8.2:32725623-32725642 MS.gene93060:CDS 40.0%
! TTTTGCTCCATGAAGAATGG+AGG + chr8.2:32725626-32725645 MS.gene93060:CDS 40.0%
!! AGCATAGGTTGGAAGTGTTT+AGG + chr8.2:32725768-32725787 MS.gene93060:CDS 40.0%
!! TAATATGGGGGTGGATTTTG+TGG + chr8.2:32724377-32724396 MS.gene93060:CDS 40.0%
AAAGGGGATCAAGCATAGGT+TGG + chr8.2:32725757-32725776 MS.gene93060:CDS 45.0%
AGAAGAAGTTTGAGAAGGGG+AGG - chr8.2:32723989-32724008 None:intergenic 45.0%
AGAAGAGGTTGGATGAGGAT+AGG + chr8.2:32724676-32724695 MS.gene93060:CDS 45.0%
AGAAGGGGAGGTAATTGAAG+GGG - chr8.2:32723977-32723996 None:intergenic 45.0%
ATAATCTATACCGGCCAAGG+AGG + chr8.2:32725092-32725111 MS.gene93060:CDS 45.0%
ATGACGAGTTCTCTCAATGG+AGG - chr8.2:32726172-32726191 None:intergenic 45.0%
ATGGAGGGCGAAATGTTGAA+TGG + chr8.2:32724777-32724796 MS.gene93060:CDS 45.0%
ATTGGTTGAGGAGAAGAGGT+TGG + chr8.2:32724665-32724684 MS.gene93060:CDS 45.0%
CAACAACCTCATTTCCCTTC+TGG + chr8.2:32724138-32724157 MS.gene93060:CDS 45.0%
CCGTCTCATGCTATATGCAA+TGG + chr8.2:32726138-32726157 MS.gene93060:CDS 45.0%
CCTAAAAGTAGGTCTCCCAA+TGG + chr8.2:32724996-32725015 MS.gene93060:CDS 45.0%
CGCGATACAACCATAACTCT+CGG - chr8.2:32724860-32724879 None:intergenic 45.0%
CTCAAAAGGGGATCAAGCAT+AGG + chr8.2:32725753-32725772 MS.gene93060:CDS 45.0%
GACTGAGTTCTCCATTGAAG+CGG - chr8.2:32724325-32724344 None:intergenic 45.0%
GAGAAGGGGAGGTAATTGAA+GGG - chr8.2:32723978-32723997 None:intergenic 45.0%
GATGATTTCTTCACCACCCA+AGG + chr8.2:32724108-32724127 MS.gene93060:CDS 45.0%
GCAACATGATGAGGAGAACA+AGG + chr8.2:32724538-32724557 MS.gene93060:CDS 45.0%
GCATTACGGGATCGAGATTA+GGG + chr8.2:32725190-32725209 MS.gene93060:CDS 45.0%
GCCTTTATCTGCGAGTATAC+GGG + chr8.2:32725842-32725861 MS.gene93060:CDS 45.0%
GGATTGAGGGTTGACATGTT+AGG - chr8.2:32724021-32724040 None:intergenic 45.0%
GGGTTGACATGTTAGGGATT+TGG - chr8.2:32724014-32724033 None:intergenic 45.0%
GTGTTTAGGTCTGCACAAAC+TGG + chr8.2:32725782-32725801 MS.gene93060:CDS 45.0%
TAGATAGATGGGCAGAATGG+GGG + chr8.2:32725939-32725958 MS.gene93060:CDS 45.0%
TAGGTCTGCACAAACTGGTT+GGG + chr8.2:32725787-32725806 MS.gene93060:CDS 45.0%
TCCCGTATACTCGCAGATAA+AGG - chr8.2:32725846-32725865 None:intergenic 45.0%
TCGTGGGATGAAGTATGAAG+GGG + chr8.2:32725217-32725236 MS.gene93060:CDS 45.0%
TGACGAGTTCTCTCAATGGA+GGG - chr8.2:32726171-32726190 None:intergenic 45.0%
TGAGAAGGGGAGGTAATTGA+AGG - chr8.2:32723979-32723998 None:intergenic 45.0%
TGCATTACGGGATCGAGATT+AGG + chr8.2:32725189-32725208 MS.gene93060:CDS 45.0%
TGGGAGGACAAGAGCAAAAA+GGG - chr8.2:32725715-32725734 None:intergenic 45.0%
TGGTGTGTTCATATCCAGGA+GGG - chr8.2:32724191-32724210 None:intergenic 45.0%
TGTGGAGGCAGATAAGAGAT+TGG + chr8.2:32724647-32724666 MS.gene93060:CDS 45.0%
TTAGGTCTGCACAAACTGGT+TGG + chr8.2:32725786-32725805 MS.gene93060:CDS 45.0%
TTGCATATAGCATGAGACGG+GGG - chr8.2:32726138-32726157 None:intergenic 45.0%
TTGGGAGGACAAGAGCAAAA+AGG - chr8.2:32725716-32725735 None:intergenic 45.0%
TTGGTGTGTTCATATCCAGG+AGG - chr8.2:32724192-32724211 None:intergenic 45.0%
! AGAGATTGGTTGAGGAGAAG+AGG + chr8.2:32724661-32724680 MS.gene93060:CDS 45.0%
! AGCTATTGGCTGTGAATGTG+TGG + chr8.2:32725583-32725602 MS.gene93060:CDS 45.0%
! AGGTTGTTGTTGGGAACCTT+GGG - chr8.2:32724127-32724146 None:intergenic 45.0%
! CAATGCTTATGCCGCTTCAA+TGG + chr8.2:32724311-32724330 MS.gene93060:CDS 45.0%
! CCTTTTGAGCAACACGATTC+CGG - chr8.2:32725742-32725761 None:intergenic 45.0%
! CGGAATCGTGTTGCTCAAAA+GGG + chr8.2:32725740-32725759 MS.gene93060:CDS 45.0%
! CTTCAGGGTTTGCTTGTGAA+AGG + chr8.2:32725665-32725684 MS.gene93060:CDS 45.0%
! GGAATCGTGTTGCTCAAAAG+GGG + chr8.2:32725741-32725760 MS.gene93060:CDS 45.0%
! GGCAGATAAGAGATTGGTTG+AGG + chr8.2:32724653-32724672 MS.gene93060:CDS 45.0%
! TATTGGCTGTGAATGTGTGG+AGG + chr8.2:32725586-32725605 MS.gene93060:CDS 45.0%
! TGAAGCGGCATAAGCATTGT+TGG - chr8.2:32724310-32724329 None:intergenic 45.0%
! TGGTGATGAGAACATGGAAG+AGG + chr8.2:32724743-32724762 MS.gene93060:CDS 45.0%
!! AGGATAGTTGGTTCGATACC+TGG + chr8.2:32724885-32724904 MS.gene93060:CDS 45.0%
!! CCATTGGGAGACCTACTTTT+AGG - chr8.2:32724999-32725018 None:intergenic 45.0%
!! GCATACTACGCTCTAAAGCA+AGG - chr8.2:32725172-32725191 None:intergenic 45.0%
ACATGGAAGAGGCAGCAACA+TGG + chr8.2:32724754-32724773 MS.gene93060:CDS 50.0%
AGATAGATGGGCAGAATGGG+GGG + chr8.2:32725940-32725959 MS.gene93060:CDS 50.0%
AGCAACACGATTCCGGCAAT+TGG - chr8.2:32725735-32725754 None:intergenic 50.0%
AGGCAAGTCAGATGCTTGTG+AGG + chr8.2:32724478-32724497 MS.gene93060:CDS 50.0%
AGGTCTGCACAAACTGGTTG+GGG + chr8.2:32725788-32725807 MS.gene93060:CDS 50.0%
ATCATGTTCGGCAGTAGCTC+TGG + chr8.2:32724446-32724465 MS.gene93060:CDS 50.0%
CAACTCACTCCCATTTCCAC+CGG + chr8.2:32724042-32724061 MS.gene93060:CDS 50.0%
CACAAACTGGTTGGGGAGTT+AGG + chr8.2:32725795-32725814 MS.gene93060:CDS 50.0%
CATCAGAGCGGGAAAGCTAT+TGG + chr8.2:32725569-32725588 MS.gene93060:CDS 50.0%
CGTGGGATGAAGTATGAAGG+GGG + chr8.2:32725218-32725237 MS.gene93060:CDS 50.0%
CTTCTTTCTTCCTCCTTGGC+CGG - chr8.2:32725105-32725124 None:intergenic 50.0%
GCAACACGATTCCGGCAATT+GGG - chr8.2:32725734-32725753 None:intergenic 50.0%
GCGATTATGGAGACGGAGAT+GGG + chr8.2:32724717-32724736 MS.gene93060:CDS 50.0%
GGGACGAGTATGGTTTGAAG+CGG - chr8.2:32724072-32724091 None:intergenic 50.0%
GGTGGAAATGGGAGTGAGTT+GGG - chr8.2:32724043-32724062 None:intergenic 50.0%
GTGGAAATGGGAGTGAGTTG+GGG - chr8.2:32724042-32724061 None:intergenic 50.0%
TGAGGAGAAGAGGTTGGATG+AGG + chr8.2:32724671-32724690 MS.gene93060:CDS 50.0%
TGCGATTATGGAGACGGAGA+TGG + chr8.2:32724716-32724735 MS.gene93060:CDS 50.0%
TGTGTGGAGGCTTGTACTGA+TGG + chr8.2:32725599-32725618 MS.gene93060:CDS 50.0%
! ATGGGCTGCGAGGTTAATGA+AGG + chr8.2:32724833-32724852 MS.gene93060:CDS 50.0%
! CCGGAATCGTGTTGCTCAAA+AGG + chr8.2:32725739-32725758 MS.gene93060:CDS 50.0%
! GAGGTTGTTGTTGGGAACCT+TGG - chr8.2:32724128-32724147 None:intergenic 50.0%
! GGCTGACGAAGTTTTTGTGG+AGG + chr8.2:32724632-32724651 MS.gene93060:CDS 50.0%
! TATGGTTTGAAGCGGTGCTC+CGG - chr8.2:32724064-32724083 None:intergenic 50.0%
! TTGTTGTTGGGAACCTTGGG+TGG - chr8.2:32724124-32724143 None:intergenic 50.0%
!! AGTAGCAATTGGCTCGCCAT+TGG - chr8.2:32725015-32725034 None:intergenic 50.0%
!! GTAGCAATTGGCTCGCCATT+GGG - chr8.2:32725014-32725033 None:intergenic 50.0%
ACACGATTCCGGCAATTGGG+AGG - chr8.2:32725731-32725750 None:intergenic 55.0%
ACTATGGCATCCCTGACGAG+TGG + chr8.2:32726194-32726213 MS.gene93060:CDS 55.0%
AGAGGCAGCAACATGGATGG+AGG + chr8.2:32724761-32724780 MS.gene93060:CDS 55.0%
CGGTGGAAATGGGAGTGAGT+TGG - chr8.2:32724044-32724063 None:intergenic 55.0%
CTATGGCATCCCTGACGAGT+GGG + chr8.2:32726195-32726214 MS.gene93060:CDS 55.0%
GAGGCAGCAACATGGATGGA+GGG + chr8.2:32724762-32724781 MS.gene93060:CDS 55.0%
GCTCTTGTCCTCCCAATTGC+CGG + chr8.2:32725720-32725739 MS.gene93060:CDS 55.0%
GGAAGAGGCAGCAACATGGA+TGG + chr8.2:32724758-32724777 MS.gene93060:CDS 55.0%
TGGTTGGGGAGTTAGGTCCT+TGG + chr8.2:32725802-32725821 MS.gene93060:CDS 55.0%
! GGGAGCTGGTGATGAGAACA+TGG + chr8.2:32724737-32724756 MS.gene93060:CDS 55.0%
! GGGAGTGAGTTGGGGATTGA+GGG - chr8.2:32724034-32724053 None:intergenic 55.0%
! TGGGAGTGAGTTGGGGATTG+AGG - chr8.2:32724035-32724054 None:intergenic 55.0%
AAGCGGTGCTCCGGTGGAAA+TGG - chr8.2:32724055-32724074 None:intergenic 60.0%
AGCGGTGCTCCGGTGGAAAT+GGG - chr8.2:32724054-32724073 None:intergenic 60.0%
ATGGAGACGGAGATGGGAGC+TGG + chr8.2:32724723-32724742 MS.gene93060:CDS 60.0%
! GTGGTGACATATGGGCTGCG+AGG + chr8.2:32724823-32724842 MS.gene93060:CDS 60.0%
!! GGGTGCGTCTAGCTCTGGTA+AGG + chr8.2:32725238-32725257 MS.gene93060:CDS 60.0%
TGGCATCCCTGACGAGTGGG+CGG + chr8.2:32726198-32726217 MS.gene93060:CDS 65.0%
! GAAGGGGGTGCGTCTAGCTC+TGG + chr8.2:32725233-32725252 MS.gene93060:CDS 65.0%
! GGTTTGAAGCGGTGCTCCGG+TGG - chr8.2:32724061-32724080 None:intergenic 65.0%
AGCTTGCCCGCCCACTCGTC+AGG - chr8.2:32726208-32726227 None:intergenic 70.0%
GCTTGCCCGCCCACTCGTCA+GGG - chr8.2:32726207-32726226 None:intergenic 70.0%
GGCATCCCTGACGAGTGGGC+GGG + chr8.2:32726199-32726218 MS.gene93060:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 32723937 32726243 32723937 ID=MS.gene93060
chr8.2 mRNA 32723937 32726243 32723937 ID=MS.gene93060.t1;Parent=MS.gene93060
chr8.2 exon 32723937 32726243 32723937 ID=MS.gene93060.t1.exon1;Parent=MS.gene93060.t1
chr8.2 CDS 32723937 32726243 32723937 ID=cds.MS.gene93060.t1;Parent=MS.gene93060.t1
Gene Sequence

>MS.gene93060

ATGAATCCAATAGATCCTTTATCATTAACCCTTGAAACCCCTTCAATTACCTCCCCTTCTCAAACTTCTTCTCACCAAATCCCTAACATGTCAACCCTCAATCCCCAACTCACTCCCATTTCCACCGGAGCACCGCTTCAAACCATACTCGTCCCTAAACCTAATCATTTTGATGATTTCTTCACCACCCAAGGTTCCCAACAACAACCTCATTTCCCTTCTGGATATGAACACAACAATCAATATCATTTCCCTCCTGGATATGAACACACCAATCAATCTAACCTCTACACTAAGTACTACAACATTCCACAACCTTCTAATGCTACTTTCGATAAATCCCTTTATCATTTTCTCAAAATCGATGATTCCAACAATGCTTATGCCGCTTCAATGGAGAACTCAGTCAATATTTCTGACCTTGGACTAAATCATAACCTTAATATGGGGGTGGATTTTGTGGATGATAGAAATGATCGATCTATTGTGCCCATTTCAAATAAACAAGAATCATGTTCGGCAGTAGCTCTGGTTAAGAAAAAGGCAAGTCAGATGCTTGTGAGGTATTCAAATATGAGTATGAAGGATCGGATGCATTTTTGCAACATGATGAGGAGAACAAGGATGACTTTTGATTCGCTACGTGTGTTAGCAAGTATTGAGGATGAGAAAAGAATTATTGAGGAGAAGAAATTGGCTGACGAAGTTTTTGTGGAGGCAGATAAGAGATTGGTTGAGGAGAAGAGGTTGGATGAGGATAGGATATTTGTTTCTGAAGTTGCGATTATGGAGACGGAGATGGGAGCTGGTGATGAGAACATGGAAGAGGCAGCAACATGGATGGAGGGCGAAATGTTGAATGGAAAAAGACGTTATAATCATATTCGTGGTGACATATGGGCTGCGAGGTTAATGAAGGACCGAGAGTTATGGTTGTATCGCGATAAAAGGATAGTTGGTTCGATACCTGGAGTTTATGTTGGTGATGTTTTCTTGTATATGGTGGAATTATGTGTAATTGGTTTGCATATGCAGATACAAGCTGGCATTGATTTTCTTCCTAAAAGTAGGTCTCCCAATGGCGAGCCAATTGCTACTAGTGTGATTGTTTCAAGTGGTTATGAGGATGATATGGAATTGGATAATGGTGATGTAATAATCTATACCGGCCAAGGAGGAAGAAAGAAGAATTCCTCAATTCAAATTTGTGATCAGAAATTAGAGGGTGGAAACCTTGCTTTAGAGCGTAGTATGCATTACGGGATCGAGATTAGGGTTATTCGTGGGATGAAGTATGAAGGGGGTGCGTCTAGCTCTGGTAAGGTTTATGTTTATGATGGATTGTATAGGATTATTGATTGCTGGTTTGAAGAAGGGATGAATGGTTTTAGAGTTTTTAAGTATAAGTTATTGAGAGTTGAAGGGCAAACTAAGATGGGTAGTGTTGTTCTCAAAGATGCTAGGAAGATTAAGAAAATTGGGTTGGATGTTAAGCCTATGAATTGTCTTTCTATTGATATTTCACACAAGAGGGAGAATGTTCCTATTAGGCTATTTAATGACATAGATGACAATGAAGAACCTCTTAATTATGAGTATCTTCCAAAAACTATTTTTCCGTGGTTTGTATTTCATCAGAGCGGGAAAGCTATTGGCTGTGAATGTGTGGAGGCTTGTACTGATGGCTGCTTTTGCTCCATGAAGAATGGAGGAGAGTTTCCCTATAATCTTCAGGGTTTGCTTGTGAAAGGTAAGCCTTTGATTTTTGAATGTGGCCCTTTTTGCTCTTGTCCTCCCAATTGCCGGAATCGTGTTGCTCAAAAGGGGATCAAGCATAGGTTGGAAGTGTTTAGGTCTGCACAAACTGGTTGGGGAGTTAGGTCCTTGGACCTTATTCAAGTTGGAGCCTTTATCTGCGAGTATACGGGAGTAATTTTAACAAGGGAACAAGCTCAAATTTTAACAATGAATGGCGACTCGTTAATATATCCAAAAAGGTTTATAGATAGATGGGCAGAATGGGGGGATTTGTCTCAGATATACAATAATTATGTTCGTCCATCATATCCATTAATACCTGATATAGATTATTCTATGGATATATCAACACTGCGAAATGTTGCTAGCTATTTGAGCCATAGTTCGAGTCCAAATGTATTTGTTCAGTTTGTTCTCCATGATCATAACAATCTGATGTTCCCCCGTCTCATGCTATATGCAATGGAGAACATCCCTCCATTGAGAGAACTCGTCATAGACTATGGCATCCCTGACGAGTGGGCGGGCAAGCTATCAGTATGTATGTAA
Protein sequence

>MS.gene93060.t1

MNPIDPLSLTLETPSITSPSQTSSHQIPNMSTLNPQLTPISTGAPLQTILVPKPNHFDDFFTTQGSQQQPHFPSGYEHNNQYHFPPGYEHTNQSNLYTKYYNIPQPSNATFDKSLYHFLKIDDSNNAYAASMENSVNISDLGLNHNLNMGVDFVDDRNDRSIVPISNKQESCSAVALVKKKASQMLVRYSNMSMKDRMHFCNMMRRTRMTFDSLRVLASIEDEKRIIEEKKLADEVFVEADKRLVEEKRLDEDRIFVSEVAIMETEMGAGDENMEEAATWMEGEMLNGKRRYNHIRGDIWAARLMKDRELWLYRDKRIVGSIPGVYVGDVFLYMVELCVIGLHMQIQAGIDFLPKSRSPNGEPIATSVIVSSGYEDDMELDNGDVIIYTGQGGRKKNSSIQICDQKLEGGNLALERSMHYGIEIRVIRGMKYEGGASSSGKVYVYDGLYRIIDCWFEEGMNGFRVFKYKLLRVEGQTKMGSVVLKDARKIKKIGLDVKPMNCLSIDISHKRENVPIRLFNDIDDNEEPLNYEYLPKTIFPWFVFHQSGKAIGCECVEACTDGCFCSMKNGGEFPYNLQGLLVKGKPLIFECGPFCSCPPNCRNRVAQKGIKHRLEVFRSAQTGWGVRSLDLIQVGAFICEYTGVILTREQAQILTMNGDSLIYPKRFIDRWAEWGDLSQIYNNYVRPSYPLIPDIDYSMDISTLRNVASYLSHSSSPNVFVQFVLHDHNNLMFPRLMLYAMENIPPLRELVIDYGIPDEWAGKLSVCM