AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93094

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93094.t1 MTR_8g098275 96.037 429 12 1 1 429 6 429 0.0 851
MS.gene93094.t1 MTR_8g098275 96.815 314 10 0 116 429 23 336 0.0 630
MS.gene93094.t1 MTR_8g098275 97.059 272 8 0 158 429 1 272 0.0 548
MS.gene93094.t1 MTR_1g072320 54.945 182 76 2 1 182 11 186 4.83e-62 212
MS.gene93094.t1 MTR_5g030430 35.260 173 97 5 14 181 60 222 2.10e-25 109
MS.gene93094.t1 MTR_5g030420 31.579 190 115 6 1 184 28 208 1.04e-24 102
MS.gene93094.t1 MTR_5g030420 31.579 190 115 6 1 184 28 208 2.09e-24 102
MS.gene93094.t1 MTR_8g027495 34.239 184 107 5 9 185 66 242 1.87e-19 91.3
MS.gene93094.t1 MTR_8g067280 29.297 256 144 11 14 253 69 303 1.99e-19 91.3
MS.gene93094.t1 MTR_7g078400 72.000 50 13 1 1 49 18 67 3.61e-16 73.2
MS.gene93094.t1 MTR_7g117670 31.053 190 103 6 14 185 67 246 1.44e-14 76.3
MS.gene93094.t1 MTR_7g096350 29.000 200 113 5 1 189 50 231 1.23e-13 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93094.t1 AT1G63650 44.912 452 197 11 1 430 13 434 4.38e-122 367
MS.gene93094.t1 AT1G63650 44.912 452 197 11 1 430 13 434 4.38e-122 367
MS.gene93094.t1 AT1G63650 44.912 452 197 11 1 430 13 434 4.38e-122 367
MS.gene93094.t1 AT5G41315 43.515 478 201 15 1 430 14 470 6.68e-118 358
MS.gene93094.t1 AT5G41315 43.515 478 201 15 1 430 22 478 7.09e-118 358
MS.gene93094.t1 AT5G41315 43.515 478 201 15 1 430 19 475 8.01e-118 358
MS.gene93094.t1 AT4G00480 53.030 198 76 3 1 184 23 217 9.74e-61 206
MS.gene93094.t1 AT4G00480 53.030 198 76 3 1 184 23 217 1.49e-60 206
MS.gene93094.t1 AT4G00480 53.030 198 76 3 1 184 23 217 6.60e-60 205
MS.gene93094.t1 AT4G09820 50.562 178 86 2 5 182 24 199 1.13e-55 192
MS.gene93094.t1 AT1G32640 32.948 173 105 4 14 181 83 249 6.26e-22 99.0
MS.gene93094.t1 AT1G01260 35.263 190 110 5 14 198 66 247 9.86e-22 98.2
MS.gene93094.t1 AT1G01260 35.263 190 110 5 14 198 66 247 9.86e-22 98.2
MS.gene93094.t1 AT1G01260 35.263 190 110 5 14 198 66 247 9.86e-22 98.2
MS.gene93094.t1 AT4G17880 30.168 179 107 4 14 182 78 248 8.93e-21 95.5
MS.gene93094.t1 AT2G46510 32.022 178 106 4 14 182 66 237 4.64e-20 93.2
MS.gene93094.t1 AT5G46830 32.000 175 111 4 14 181 49 222 2.23e-17 84.7
MS.gene93094.t1 AT4G16430 30.570 193 115 7 1 187 50 229 1.28e-16 82.0
MS.gene93094.t1 AT5G46760 29.885 174 106 4 14 180 67 231 3.71e-15 78.2

Find 71 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAAAATGGCCAACCAATT+TGG 0.253067 4.2:+6420368 MS.gene93094:CDS
TCAGCATCAATTAGCCAAAT+TGG 0.260352 4.2:-6420382 None:intergenic
CAAACACAAAAGCTTGATTC+TGG 0.267090 4.2:-6421778 None:intergenic
AGATACCGAGTGGTACTATT+TGG 0.275916 4.2:+6420185 MS.gene93094:CDS
ATTATGTCATATCCAACTTC+TGG 0.286568 4.2:-6421439 None:intergenic
AATGTAGCTCGAGCGTTAAT+AGG 0.287585 4.2:-6421346 None:intergenic
AAAGTTATTGAAGAAGATTT+TGG 0.310498 4.2:+6421852 MS.gene93094:CDS
AGGTCCCTTGTCTTGGCCTT+TGG 0.323833 4.2:-6421825 None:intergenic
GAACTTAGTTCTGACGAAAT+AGG 0.323945 4.2:+6420054 MS.gene93094:CDS
GGTTGAAAGCTGGCAAGTTT+TGG 0.326038 4.2:+6421534 MS.gene93094:CDS
AATATTACCTTACTATTTGA+AGG 0.331430 4.2:-6422042 None:intergenic
CCAGGAAGAGCTCTAGAAAA+TGG 0.340134 4.2:+6420355 MS.gene93094:CDS
TGCTCAGAGTGCATCAATTC+AGG 0.344767 4.2:+6420683 MS.gene93094:intron
TAATGCCAATCTTCACTTAG+AGG 0.362258 4.2:-6421682 None:intergenic
AATGCATCTCCATTGTTAAC+TGG 0.375501 4.2:-6421741 None:intergenic
TTGCAATCTTCTCCAACCTT+AGG 0.396557 4.2:-6421631 None:intergenic
GTTGAATTAATTCCAGAAGT+TGG 0.399420 4.2:+6421427 MS.gene93094:CDS
AATGCATTTGAGCTACCATT+AGG 0.406315 4.2:-6421478 None:intergenic
TCAACCATGAATGTTTCATT+TGG 0.414054 4.2:-6421517 None:intergenic
AAATATTTCTTCTGCTCCTA+AGG 0.414742 4.2:+6421615 MS.gene93094:CDS
CATGAAGGAGCAACTTGCTT+TGG 0.415492 4.2:+6419271 None:intergenic
TGTTTCATTTGGTAGTTGAT+TGG 0.427906 4.2:-6421506 None:intergenic
ACTACCAAATGAAACATTCA+TGG 0.431417 4.2:+6421513 MS.gene93094:CDS
ATGTAGCTCGAGCGTTAATA+GGG 0.437263 4.2:-6421345 None:intergenic
ATACCAACCCTCAAACAAAA+AGG 0.440617 4.2:+6420130 MS.gene93094:CDS
TCGGTATCTGTGAGATCATC+AGG 0.444790 4.2:-6420171 None:intergenic
AATGGAGCTATGTAATATTC+TGG 0.445432 4.2:+6419309 MS.gene93094:CDS
AATGGTTCCTTCAAATAGTA+AGG 0.449741 4.2:+6422035 MS.gene93094:CDS
ATTTCTTCTGCTCCTAAGGT+TGG 0.458378 4.2:+6421619 MS.gene93094:CDS
CTTGGCCTTTGGAAATTCAC+AGG 0.474066 4.2:-6421814 None:intergenic
AACACAGCAACCTCTCCTAA+TGG 0.474418 4.2:+6421463 MS.gene93094:CDS
ATGAAAGGGGTTATTGAGCT+AGG 0.483817 4.2:+6421128 MS.gene93094:CDS
TGGCAGTTAGAAGCATTCAA+TGG 0.489433 4.2:+6419291 MS.gene93094:CDS
TGGATCCTCTAAGTGAAGAT+TGG 0.499708 4.2:+6421677 MS.gene93094:CDS
TTGTGTGTTCTTAGTTTACC+AGG 0.508462 4.2:+6420337 MS.gene93094:intron
TATGCAGTCACTTGAATTCA+CGG 0.508466 4.2:-6421587 None:intergenic
TTCGTATTCAACATTGGCCA+AGG 0.510865 4.2:+6420219 MS.gene93094:CDS
TCCTTCTTTCTGACAAAACA+TGG 0.517100 4.2:-6421972 None:intergenic
AGTTTGTATCTTACCCTGGT+TGG 0.519375 4.2:-6419346 None:intergenic
TCAATAACCCCTTTCATGAA+AGG 0.530003 4.2:-6421122 None:intergenic
AAACATTCATGGTTGAAAGC+TGG 0.541473 4.2:+6421524 MS.gene93094:CDS
CATCAATTAGCCAAATTGGT+TGG 0.542518 4.2:-6420378 None:intergenic
GAAGAGCAAAGCTAAATGAA+AGG 0.559544 4.2:+6421995 MS.gene93094:CDS
TCTGAATCTGTTAACCAACC+AGG 0.589945 4.2:+6419332 MS.gene93094:CDS
TAGTCGCGCTCTCCTCGCAA+AGG 0.589968 4.2:+6420426 MS.gene93094:CDS
TGGTTTGCTTGAATCTCAAG+AGG 0.591791 4.2:+6421900 MS.gene93094:CDS
GTTCCACGGATGCACACTGA+TGG 0.593993 4.2:+6421880 MS.gene93094:CDS
ACAAGACTAGAGGCTGATGA+AGG 0.599190 4.2:+6421943 MS.gene93094:CDS
AATTTCCAAAGGCCAAGACA+AGG 0.606654 4.2:+6421820 MS.gene93094:CDS
ACCGAAAAGACTTACGTGAT+CGG 0.607356 4.2:-6421156 None:intergenic
ATGTCCTTCGTATTCAACAT+TGG 0.611258 4.2:+6420213 MS.gene93094:CDS
GAGGAGAATGACTATAGAGA+AGG 0.612043 4.2:+6421919 MS.gene93094:CDS
TCAGGTGAAAGTGCTGCAGA+AGG 0.614503 4.2:-6420153 None:intergenic
AAGACAAGGAAAACAAGTCA+AGG 0.617178 4.2:+6420027 MS.gene93094:CDS
AAACCATCAGTGTGCATCCG+TGG 0.620753 4.2:-6421883 None:intergenic
TCGTATTCAACATTGGCCAA+GGG 0.620796 4.2:+6420220 MS.gene93094:CDS
ATTTGAGCTACCATTAGGAG+AGG 0.628279 4.2:-6421473 None:intergenic
AAGCAATGACCAGTTAACAA+TGG 0.630188 4.2:+6421732 MS.gene93094:CDS
AAGGAAAACAAGTCAAGGAG+TGG 0.630274 4.2:+6420032 MS.gene93094:CDS
CTGAATCTGTTAACCAACCA+GGG 0.631174 4.2:+6419333 MS.gene93094:CDS
TAGAGAAGGTACAAGACTAG+AGG 0.643862 4.2:+6421933 MS.gene93094:CDS
GTGTGCCTGTGAATTTCCAA+AGG 0.646463 4.2:+6421809 MS.gene93094:CDS
TTTCAGGGTGTTGAGCTGGG+GGG 0.650923 4.2:+6419981 MS.gene93094:intron
GAGAAACATATACCTTTGCG+AGG 0.658095 4.2:-6420438 None:intergenic
ATTTCCAAAGGCCAAGACAA+GGG 0.685503 4.2:+6421821 MS.gene93094:CDS
TTGGCCAATGTTGAATACGA+AGG 0.685744 4.2:-6420217 None:intergenic
GCAAACCAAATAGTACCACT+CGG 0.699870 4.2:-6420190 None:intergenic
CTGACGAAATAGGCTTACAG+AGG 0.712274 4.2:+6420064 MS.gene93094:CDS
TTGAATTCGAGAAATAACGA+AGG 0.725975 4.2:+6421367 MS.gene93094:CDS
ACAATGGAGAGATTAAGACA+AGG 0.747987 4.2:+6420013 MS.gene93094:CDS
ATGATCTCACAGATACCGAG+TGG 0.748813 4.2:+6420175 MS.gene93094:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAACTATTAATAACTAT+TGG - chr4.2:6420761-6420780 None:intergenic 15.0%
!! ATTAATGTTATAAAAGACAA+TGG - chr4.2:6421186-6421205 None:intergenic 15.0%
!! TTTGGAAATTAATAGAATAA+AGG - chr4.2:6420986-6421005 None:intergenic 15.0%
!! AAATTTACATGTGTTAAGAT+AGG + chr4.2:6420806-6420825 MS.gene93094:intron 20.0%
!! AATATTACCTTACTATTTGA+AGG - chr4.2:6422045-6422064 None:intergenic 20.0%
!! TGTACAATTTAAAAACAGAA+AGG - chr4.2:6420290-6420309 None:intergenic 20.0%
!!! AAAGTTATTGAAGAAGATTT+TGG + chr4.2:6421852-6421871 MS.gene93094:CDS 20.0%
!!! GATTTTTGGTTTCTAAAATT+TGG - chr4.2:6421042-6421061 None:intergenic 20.0%
! AAACATGAACATATTCAAGA+AGG - chr4.2:6419424-6419443 None:intergenic 25.0%
! AACATTATTACAAGAAGTTG+AGG - chr4.2:6421230-6421249 None:intergenic 25.0%
! ACATTATTACAAGAAGTTGA+GGG - chr4.2:6421229-6421248 None:intergenic 25.0%
! ATCCATCCTTATTACTATTT+GGG + chr4.2:6420882-6420901 MS.gene93094:intron 25.0%
! ATGCTTAAGTCAATAATTGA+GGG - chr4.2:6419742-6419761 None:intergenic 25.0%
! CATATAAAGTCTTAGTTTCA+AGG - chr4.2:6419455-6419474 None:intergenic 25.0%
! GGTGTACTTATAAAAAGAAA+GGG + chr4.2:6420552-6420571 MS.gene93094:intron 25.0%
! TAACATTTATCTTCTTCCTT+TGG + chr4.2:6420622-6420641 MS.gene93094:intron 25.0%
! TAGCATAAACACATTTAATG+AGG - chr4.2:6419387-6419406 None:intergenic 25.0%
! TATCCATCCTTATTACTATT+TGG + chr4.2:6420881-6420900 MS.gene93094:intron 25.0%
! TATGCTTAAGTCAATAATTG+AGG - chr4.2:6419743-6419762 None:intergenic 25.0%
! TGGAGTTCATTTATAATACA+AGG - chr4.2:6420741-6420760 None:intergenic 25.0%
!! AAATTACACAATGCATTGAT+AGG - chr4.2:6421064-6421083 None:intergenic 25.0%
!! ACATGTCATAAATTGTTTTC+AGG + chr4.2:6419802-6419821 MS.gene93094:intron 25.0%
!! CATCTAAGATAACAAATAAG+TGG - chr4.2:6419577-6419596 None:intergenic 25.0%
!! TATAAAGGCGTGTATAAAAA+GGG - chr4.2:6420520-6420539 None:intergenic 25.0%
!! TTATAAAGGCGTGTATAAAA+AGG - chr4.2:6420521-6420540 None:intergenic 25.0%
!!! AGATTATTAGTCTTTAGGTT+TGG - chr4.2:6421004-6421023 None:intergenic 25.0%
!!! GTCTTAGATTATTAGTCTTT+AGG - chr4.2:6421009-6421028 None:intergenic 25.0%
!!! GTTTTTAACTCTTAGATCAA+TGG + chr4.2:6422017-6422036 MS.gene93094:CDS 25.0%
AAAAGATTGAGATTTACCCT+TGG - chr4.2:6420239-6420258 None:intergenic 30.0%
AAATATTTCTTCTGCTCCTA+AGG + chr4.2:6421615-6421634 MS.gene93094:CDS 30.0%
AATGGAGCTATGTAATATTC+TGG + chr4.2:6419309-6419328 MS.gene93094:CDS 30.0%
AATGGTTCCTTCAAATAGTA+AGG + chr4.2:6422035-6422054 MS.gene93094:CDS 30.0%
ACTAAGAACACACAAATTTG+AGG - chr4.2:6420332-6420351 None:intergenic 30.0%
ACTACCAAATGAAACATTCA+TGG + chr4.2:6421513-6421532 MS.gene93094:CDS 30.0%
ACTAGAAAGGAACACAAAAA+AGG - chr4.2:6420264-6420283 None:intergenic 30.0%
ACTTCTTGTAATAATGTTGC+TGG + chr4.2:6421232-6421251 MS.gene93094:intron 30.0%
AGATCATCTTAGAGAGTTTA+TGG + chr4.2:6419767-6419786 MS.gene93094:intron 30.0%
ATTATGTCATATCCAACTTC+TGG - chr4.2:6421442-6421461 None:intergenic 30.0%
GCCTTTATAAACTCACAAAA+GGG + chr4.2:6420531-6420550 MS.gene93094:intron 30.0%
GGGTGTACTTATAAAAAGAA+AGG + chr4.2:6420551-6420570 MS.gene93094:intron 30.0%
GTTGAATTAATTCCAGAAGT+TGG + chr4.2:6421427-6421446 MS.gene93094:CDS 30.0%
TAACCAAAAAATGAGCTTAG+TGG + chr4.2:6421657-6421676 MS.gene93094:CDS 30.0%
TATGTTTCTCTTTCTTTAGC+TGG + chr4.2:6420451-6420470 MS.gene93094:intron 30.0%
TCAACCATGAATGTTTCATT+TGG - chr4.2:6421520-6421539 None:intergenic 30.0%
TGAAAAATGGTCAAGGAAAA+AGG - chr4.2:6419966-6419985 None:intergenic 30.0%
TGTTTAAGGAACAAAACCAA+AGG - chr4.2:6420641-6420660 None:intergenic 30.0%
TTAGACTAAACACTAAAGAG+TGG - chr4.2:6420846-6420865 None:intergenic 30.0%
TTCCCAAATAGTAATAAGGA+TGG - chr4.2:6420887-6420906 None:intergenic 30.0%
TTGAATTCGAGAAATAACGA+AGG + chr4.2:6421367-6421386 MS.gene93094:CDS 30.0%
! ACCCATCTCAAATCAAATTT+TGG - chr4.2:6419488-6419507 None:intergenic 30.0%
! ACCCTTTTGTGAGTTTATAA+AGG - chr4.2:6420535-6420554 None:intergenic 30.0%
! CTTCTTCAATAACTTTTGTG+AGG - chr4.2:6421848-6421867 None:intergenic 30.0%
! TGTGTTTTCCTTTCATGAAA+GGG + chr4.2:6421114-6421133 MS.gene93094:CDS 30.0%
! TGTTTCATTTGGTAGTTGAT+TGG - chr4.2:6421509-6421528 None:intergenic 30.0%
!! CAATGCATTGATAGGATTTT+TGG - chr4.2:6421056-6421075 None:intergenic 30.0%
!! GCCAAAATTTGATTTGAGAT+GGG + chr4.2:6419484-6419503 MS.gene93094:intron 30.0%
!! TGCCAAAATTTGATTTGAGA+TGG + chr4.2:6419483-6419502 MS.gene93094:intron 30.0%
!! TTTTCCTTGACCATTTTTCA+GGG + chr4.2:6419966-6419985 MS.gene93094:intron 30.0%
!! TTTTTCCTTGACCATTTTTC+AGG + chr4.2:6419965-6419984 MS.gene93094:intron 30.0%
AAACAGTTTGTATCTTACCC+TGG - chr4.2:6419353-6419372 None:intergenic 35.0%
AAACATTCATGGTTGAAAGC+TGG + chr4.2:6421524-6421543 MS.gene93094:CDS 35.0%
AACAGAAAGGCAAACTAGAA+AGG - chr4.2:6420277-6420296 None:intergenic 35.0%
AAGACAAGGAAAACAAGTCA+AGG + chr4.2:6420027-6420046 MS.gene93094:CDS 35.0%
AAGCAATGACCAGTTAACAA+TGG + chr4.2:6421732-6421751 MS.gene93094:CDS 35.0%
AATGCATCTCCATTGTTAAC+TGG - chr4.2:6421744-6421763 None:intergenic 35.0%
AATGCATTTGAGCTACCATT+AGG - chr4.2:6421481-6421500 None:intergenic 35.0%
ACAATGGAGAGATTAAGACA+AGG + chr4.2:6420013-6420032 MS.gene93094:CDS 35.0%
ATACCAACCCTCAAACAAAA+AGG + chr4.2:6420130-6420149 MS.gene93094:CDS 35.0%
ATGTCCTTCGTATTCAACAT+TGG + chr4.2:6420213-6420232 MS.gene93094:CDS 35.0%
ATTACTATTTGGGAACCTGT+AGG + chr4.2:6420892-6420911 MS.gene93094:intron 35.0%
CAAACACAAAAGCTTGATTC+TGG - chr4.2:6421781-6421800 None:intergenic 35.0%
CAGGTTCCCAAATAGTAATA+AGG - chr4.2:6420891-6420910 None:intergenic 35.0%
CATCAATTAGCCAAATTGGT+TGG - chr4.2:6420381-6420400 None:intergenic 35.0%
CGATAAGCTGTTTATCAACA+AGG + chr4.2:6419940-6419959 MS.gene93094:intron 35.0%
CGCCTTTATAAACTCACAAA+AGG + chr4.2:6420530-6420549 MS.gene93094:intron 35.0%
GAAGAGCAAAGCTAAATGAA+AGG + chr4.2:6421995-6422014 MS.gene93094:CDS 35.0%
GACAGGTTCAAATGTGTTTA+AGG - chr4.2:6420655-6420674 None:intergenic 35.0%
TAATGCCAATCTTCACTTAG+AGG - chr4.2:6421685-6421704 None:intergenic 35.0%
TAGAAAATGGCCAACCAATT+TGG + chr4.2:6420368-6420387 MS.gene93094:CDS 35.0%
TATGCAGTCACTTGAATTCA+CGG - chr4.2:6421590-6421609 None:intergenic 35.0%
TCAATAACCCCTTTCATGAA+AGG - chr4.2:6421125-6421144 None:intergenic 35.0%
TCAGCATCAATTAGCCAAAT+TGG - chr4.2:6420385-6420404 None:intergenic 35.0%
TCCTTCTTTCTGACAAAACA+TGG - chr4.2:6421975-6421994 None:intergenic 35.0%
TGTTAAGATAGGTCTTGCTT+TGG + chr4.2:6420817-6420836 MS.gene93094:intron 35.0%
TTGTGTGTTCTTAGTTTACC+AGG + chr4.2:6420337-6420356 MS.gene93094:intron 35.0%
! ACCATGTTTTGTCAGAAAGA+AGG + chr4.2:6421971-6421990 MS.gene93094:CDS 35.0%
! ATTCTTCGCATTTTGTGTGA+AGG + chr4.2:6421255-6421274 MS.gene93094:intron 35.0%
! CAGAATCAAGCTTTTGTGTT+TGG + chr4.2:6421779-6421798 MS.gene93094:CDS 35.0%
! CTGCATTAAAGGTTTTTGCT+AGG + chr4.2:6419663-6419682 MS.gene93094:intron 35.0%
! GTGTGTTTTCCTTTCATGAA+AGG + chr4.2:6421113-6421132 MS.gene93094:CDS 35.0%
! GTGTTTTCCTTTCATGAAAG+GGG + chr4.2:6421115-6421134 MS.gene93094:CDS 35.0%
!! AGCTTTTGTGTTTGGAACAA+AGG + chr4.2:6421787-6421806 MS.gene93094:CDS 35.0%
!! GAACTTAGTTCTGACGAAAT+AGG + chr4.2:6420054-6420073 MS.gene93094:CDS 35.0%
!! GTTGGTATCTACTGTTTTGA+GGG - chr4.2:6420118-6420137 None:intergenic 35.0%
!!! GATCCACTAAGCTCATTTTT+TGG - chr4.2:6421663-6421682 None:intergenic 35.0%
AACACCCTGAAAAATGGTCA+AGG - chr4.2:6419973-6419992 None:intergenic 40.0%
AAGGAAAACAAGTCAAGGAG+TGG + chr4.2:6420032-6420051 MS.gene93094:CDS 40.0%
AAGGCGTGTATAAAAAGGGA+CGG - chr4.2:6420516-6420535 None:intergenic 40.0%
AATGTAGCTCGAGCGTTAAT+AGG - chr4.2:6421349-6421368 None:intergenic 40.0%
AATTGATGCACTCTGAGCAT+GGG - chr4.2:6420682-6420701 None:intergenic 40.0%
AATTTCCAAAGGCCAAGACA+AGG + chr4.2:6421820-6421839 MS.gene93094:CDS 40.0%
ACCGAAAAGACTTACGTGAT+CGG - chr4.2:6421159-6421178 None:intergenic 40.0%
AGATACCGAGTGGTACTATT+TGG + chr4.2:6420185-6420204 MS.gene93094:CDS 40.0%
AGTTTGTATCTTACCCTGGT+TGG - chr4.2:6419349-6419368 None:intergenic 40.0%
ATGAAAGGGGTTATTGAGCT+AGG + chr4.2:6421128-6421147 MS.gene93094:CDS 40.0%
ATGTAGCTCGAGCGTTAATA+GGG - chr4.2:6421348-6421367 None:intergenic 40.0%
ATTTCCAAAGGCCAAGACAA+GGG + chr4.2:6421821-6421840 MS.gene93094:CDS 40.0%
ATTTCTTCTGCTCCTAAGGT+TGG + chr4.2:6421619-6421638 MS.gene93094:CDS 40.0%
ATTTGAGCTACCATTAGGAG+AGG - chr4.2:6421476-6421495 None:intergenic 40.0%
CTGAATCTGTTAACCAACCA+GGG + chr4.2:6419333-6419352 MS.gene93094:CDS 40.0%
GAGAAACATATACCTTTGCG+AGG - chr4.2:6420441-6420460 None:intergenic 40.0%
GAGGAGAATGACTATAGAGA+AGG + chr4.2:6421919-6421938 MS.gene93094:CDS 40.0%
GCAAACCAAATAGTACCACT+CGG - chr4.2:6420193-6420212 None:intergenic 40.0%
TCAATGTAATTGCACGTCTC+TGG - chr4.2:6420923-6420942 None:intergenic 40.0%
TCGTATTCAACATTGGCCAA+GGG + chr4.2:6420220-6420239 MS.gene93094:CDS 40.0%
TCTGAATCTGTTAACCAACC+AGG + chr4.2:6419332-6419351 MS.gene93094:CDS 40.0%
TGGATCCTCTAAGTGAAGAT+TGG + chr4.2:6421677-6421696 MS.gene93094:CDS 40.0%
TTCGTATTCAACATTGGCCA+AGG + chr4.2:6420219-6420238 MS.gene93094:CDS 40.0%
TTGCAATCTTCTCCAACCTT+AGG - chr4.2:6421634-6421653 None:intergenic 40.0%
TTGGCCAATGTTGAATACGA+AGG - chr4.2:6420220-6420239 None:intergenic 40.0%
! ACCGATCACGTAAGTCTTTT+CGG + chr4.2:6421155-6421174 MS.gene93094:intron 40.0%
! GCTACTACTTGCTGCATTAA+AGG + chr4.2:6419652-6419671 MS.gene93094:intron 40.0%
! TAGAGAAGGTACAAGACTAG+AGG + chr4.2:6421933-6421952 MS.gene93094:CDS 40.0%
!! AGATTTTGGTCGAAGTTCCA+CGG + chr4.2:6421866-6421885 MS.gene93094:CDS 40.0%
!! CAGAAGGCCTTTTTGTTTGA+GGG - chr4.2:6420140-6420159 None:intergenic 40.0%
!! CGCATTTTGTGTGAAGGTAT+TGG + chr4.2:6421261-6421280 MS.gene93094:intron 40.0%
!! GGTTGGTATCTACTGTTTTG+AGG - chr4.2:6420119-6420138 None:intergenic 40.0%
!! TGGCAGTTAGAAGCATTCAA+TGG + chr4.2:6419291-6419310 MS.gene93094:CDS 40.0%
!! TGGTTTGCTTGAATCTCAAG+AGG + chr4.2:6421900-6421919 MS.gene93094:CDS 40.0%
!!! ATTTTTCAGGGTGTTGAGCT+GGG + chr4.2:6419978-6419997 MS.gene93094:intron 40.0%
AACACAGCAACCTCTCCTAA+TGG + chr4.2:6421463-6421482 MS.gene93094:CDS 45.0%
ACAAGACTAGAGGCTGATGA+AGG + chr4.2:6421943-6421962 MS.gene93094:CDS 45.0%
ACGTCTCTGGTATTACCTAC+AGG - chr4.2:6420910-6420929 None:intergenic 45.0%
ATGATCTCACAGATACCGAG+TGG + chr4.2:6420175-6420194 MS.gene93094:CDS 45.0%
CAGCTCAACACCCTGAAAAA+TGG - chr4.2:6419979-6419998 None:intergenic 45.0%
CTGACGAAATAGGCTTACAG+AGG + chr4.2:6420064-6420083 MS.gene93094:CDS 45.0%
GAATTGATGCACTCTGAGCA+TGG - chr4.2:6420683-6420702 None:intergenic 45.0%
GTGTGCCTGTGAATTTCCAA+AGG + chr4.2:6421809-6421828 MS.gene93094:CDS 45.0%
TCGGTATCTGTGAGATCATC+AGG - chr4.2:6420174-6420193 None:intergenic 45.0%
TCGTCTCAACTTTCAGACAG+TGG + chr4.2:6421091-6421110 MS.gene93094:intron 45.0%
TGCTCAGAGTGCATCAATTC+AGG + chr4.2:6420683-6420702 MS.gene93094:intron 45.0%
! AGCTGGCTTCTTCTTTCTTC+TGG + chr4.2:6420468-6420487 MS.gene93094:intron 45.0%
! CTTGGCCTTTGGAAATTCAC+AGG - chr4.2:6421817-6421836 None:intergenic 45.0%
! GGTTGAAAGCTGGCAAGTTT+TGG + chr4.2:6421534-6421553 MS.gene93094:CDS 45.0%
! TTTTGTGAGGTCCCTTGTCT+TGG - chr4.2:6421835-6421854 None:intergenic 45.0%
!! AGGCCTTTTTGTTTGAGGGT+TGG - chr4.2:6420136-6420155 None:intergenic 45.0%
!! CCAGGAAGAGCTCTAGAAAA+TGG + chr4.2:6420355-6420374 MS.gene93094:CDS 45.0%
!! GCAGAAGGCCTTTTTGTTTG+AGG - chr4.2:6420141-6420160 None:intergenic 45.0%
!!! CATTTTTCAGGGTGTTGAGC+TGG + chr4.2:6419977-6419996 MS.gene93094:intron 45.0%
!!! CCATTTTCTAGAGCTCTTCC+TGG - chr4.2:6420358-6420377 None:intergenic 45.0%
!!! TTTTTCAGGGTGTTGAGCTG+GGG + chr4.2:6419979-6419998 MS.gene93094:intron 45.0%
AAACCATCAGTGTGCATCCG+TGG - chr4.2:6421886-6421905 None:intergenic 50.0%
TGGGGGGAAGGATACTACAA+TGG + chr4.2:6419997-6420016 MS.gene93094:CDS 50.0%
! AGCAGCTGAGAGAGCTTTTC+AGG + chr4.2:6420091-6420110 MS.gene93094:CDS 50.0%
!! TCAGGTGAAAGTGCTGCAGA+AGG - chr4.2:6420156-6420175 None:intergenic 50.0%
!!! TTTTCAGGGTGTTGAGCTGG+GGG + chr4.2:6419980-6419999 MS.gene93094:intron 50.0%
CTCTGAGCATGGGAGAAGAC+AGG - chr4.2:6420672-6420691 None:intergenic 55.0%
GTTCCACGGATGCACACTGA+TGG + chr4.2:6421880-6421899 MS.gene93094:CDS 55.0%
! AGGTCCCTTGTCTTGGCCTT+TGG - chr4.2:6421828-6421847 None:intergenic 55.0%
!! TTTCAGGGTGTTGAGCTGGG+GGG + chr4.2:6419981-6420000 MS.gene93094:intron 55.0%
TAGTCGCGCTCTCCTCGCAA+AGG + chr4.2:6420426-6420445 MS.gene93094:CDS 60.0%
!! AGGGTGTTGAGCTGGGGGGA+AGG + chr4.2:6419985-6420004 MS.gene93094:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 6419272 6422074 6419272 ID=MS.gene93094
chr4.2 mRNA 6419272 6422074 6419272 ID=MS.gene93094.t1;Parent=MS.gene93094
chr4.2 exon 6419272 6419354 6419272 ID=MS.gene93094.t1.exon1;Parent=MS.gene93094.t1
chr4.2 CDS 6419272 6419354 6419272 ID=cds.MS.gene93094.t1;Parent=MS.gene93094.t1
chr4.2 exon 6419987 6420241 6419987 ID=MS.gene93094.t1.exon2;Parent=MS.gene93094.t1
chr4.2 CDS 6419987 6420241 6419987 ID=cds.MS.gene93094.t1;Parent=MS.gene93094.t1
chr4.2 exon 6420351 6420447 6420351 ID=MS.gene93094.t1.exon3;Parent=MS.gene93094.t1
chr4.2 CDS 6420351 6420447 6420351 ID=cds.MS.gene93094.t1;Parent=MS.gene93094.t1
chr4.2 exon 6420690 6420704 6420690 ID=MS.gene93094.t1.exon4;Parent=MS.gene93094.t1
chr4.2 CDS 6420690 6420704 6420690 ID=cds.MS.gene93094.t1;Parent=MS.gene93094.t1
chr4.2 exon 6421107 6421163 6421107 ID=MS.gene93094.t1.exon5;Parent=MS.gene93094.t1
chr4.2 CDS 6421107 6421163 6421107 ID=cds.MS.gene93094.t1;Parent=MS.gene93094.t1
chr4.2 exon 6421277 6422074 6421277 ID=MS.gene93094.t1.exon6;Parent=MS.gene93094.t1
chr4.2 CDS 6421277 6422074 6421277 ID=cds.MS.gene93094.t1;Parent=MS.gene93094.t1
Gene Sequence

>MS.gene93094

ATGAAGGAGCAACTTGCTTTGGCAGTTAGAAGCATTCAATGGAGCTATGTAATATTCTGGTCTGAATCTGTTAACCAACCAGGGTAAGATACAAACTGTTTCATTCATAGTACCTCATTAAATGTGTTTATGCTATTCTATTCTATTTTCCTTCTTGAATATGTTCATGTTTTTCTAATACCTTGAAACTAAGACTTTATATGACATTATCTGCCAAAATTTGATTTGAGATGGGTGTAAAAAAATGTTTTTTTGTGCTACTGTAGTTTTGCTTATATTAATTATCAACAGCTCTATGTTAACCACTTATTTGTTATCTTAGATGAATAGTTAACTTTTCATTTGAATCTTGAAAATCTGAATCTAGTTTTAGATTGTCTGCTACTACTTGCTGCATTAAAGGTTTTTGCTAGGAAGCTATATTCTATTCAAAATCGAGTTTGAATATGTTTTTATTGATTTATTTACCCTCAATTATTGACTTAAGCATATGTGAGATCATCTTAGAGAGTTTATGGAAATAGCTTATGACATGTCATAAATTGTTTTCAGGTTATTTCTATAAGCTCTTCATAATAGCTGATGAAAACTGATTATAGCTTCAATGAAAACAGTTTGACTTCATTTTATCTCTTGTTATAGAAATAGCTTACACATAAGCACTTATACGATAAGCTGTTTATCAACAAGGCCTTTTTCCTTGACCATTTTTCAGGGTGTTGAGCTGGGGGGAAGGATACTACAATGGAGAGATTAAGACAAGGAAAACAAGTCAAGGAGTGGAACTTAGTTCTGACGAAATAGGCTTACAGAGGAGTGAGCAGCTGAGAGAGCTTTTCAGGTCCCTCAAAACAGTAGATACCAACCCTCAAACAAAAAGGCCTTCTGCAGCACTTTCACCTGATGATCTCACAGATACCGAGTGGTACTATTTGGTTTGCATGTCCTTCGTATTCAACATTGGCCAAGGGTAAATCTCAATCTTTTTTCCTTTTTTGTGTTCCTTTCTAGTTTGCCTTTCTGTTTTTAAATTGTACATTTATTCATTCTTATTTATCCTCAAATTTGTGTGTTCTTAGTTTACCAGGAAGAGCTCTAGAAAATGGCCAACCAATTTGGCTAATTGATGCTGATTCTGTTGATTGCAAAGTGTTTAGTCGCGCTCTCCTCGCAAAGGTATATGTTTCTCTTTCTTTAGCTGGCTTCTTCTTTCTTCTGGTGTTGATTAAAATAGACTATCTCCGTCCCTTTTTATACACGCCTTTATAAACTCACAAAAGGGTGTACTTATAAAAAGAAAGGGAGATATTAGTTTTATTGTACTCTAATTGTGATTAAAATGACAAAGTCTAACATTTATCTTCTTCCTTTGGTTTTGTTCCTTAAACACATTTGAACCTGTCTTCTCCCATGCTCAGAGTGCATCAATTCAGGTATCAGTCTATTTTTTTTCTTTTCTTAATTGTCCTTGTATTATAAATGAACTCCAATAGTTATTAATAGTTTTCTTAGTACTATCAATAATAATGCAAAGAAATTTACATGTGTTAAGATAGGTCTTGCTTTGGAAACCACTCTTTAGTGTTTAGTCTAAGTACTTCTGCAATAGTATCCATCCTTATTACTATTTGGGAACCTGTAGGTAATACCAGAGACGTGCAATTACATTGAAGCTACATAGTGTAGCTTTTAAGTATATGTGTGATAAAAGCCTTTATTCTATTAATTTCCAAACCTAAAGACTAATAATCTAAGACTGATAATCGTCCAAATTTTAGAAACCAAAAATCCTATCAATGCATTGTGTAATTTGTTTCTTTCGTCTCAACTTTCAGACAGTGGTGTGTTTTCCTTTCATGAAAGGGGTTATTGAGCTAGGCACAACCGATCACGTAAGTCTTTTCGGTTTTTCCATTGTCTTTTATAACATTAATATTACTATTCTGTTATTTCTCCCTCAACTTCTTGTAATAATGTTGCTGGATTCTTCGCATTTTGTGTGAAGGTATTGGAAGATCTCAGTCTCATTCAACAGATCAAAACTTCTTTATCGAATAGTCTAAGCGTCGATGACCCTATTAACGCTCGAGCTACATTGAATTCGAGAAATAACGAAGGTGTTGCTTGTGTAGCATTTGATGATAATGATTATGATGTTGAATTAATTCCAGAAGTTGGATATGACATAATCAACACAGCAACCTCTCCTAATGGTAGCTCAAATGCATTACAAGCCAATCAACTACCAAATGAAACATTCATGGTTGAAAGCTGGCAAGTTTTGGATGATGATTTGAGCAACTGTGTTCATAACTCCGTGAATTCAAGTGACTGCATATCACAAAATATTTCTTCTGCTCCTAAGGTTGGAGAAGATTGCAATAATAACCAAAAAATGAGCTTAGTGGATCCTCTAAGTGAAGATTGGCATTATCAGAAAATTCTTTCAGAACTTTTAAAAAGCAATGACCAGTTAACAATGGAGATGCATTTTCAAAACTTTCATCCAGAATCAAGCTTTTGTGTTTGGAACAAAGGTGTGCCTGTGAATTTCCAAAGGCCAAGACAAGGGACCTCACAAAAGTTATTGAAGAAGATTTTGGTCGAAGTTCCACGGATGCACACTGATGGTTTGCTTGAATCTCAAGAGGAGAATGACTATAGAGAAGGTACAAGACTAGAGGCTGATGAAGGTATGAACCATGTTTTGTCAGAAAGAAGGAGAAGAGCAAAGCTAAATGAAAGGTTTTTAACTCTTAGATCAATGGTTCCTTCAAATAGTAAGGTAATATTTATATTTTAG
Protein sequence

>MS.gene93094.t1

MKEQLALAVRSIQWSYVIFWSESVNQPGVLSWGEGYYNGEIKTRKTSQGVELSSDEIGLQRSEQLRELFRSLKTVDTNPQTKRPSAALSPDDLTDTEWYYLVCMSFVFNIGQGLPGRALENGQPIWLIDADSVDCKVFSRALLAKSASIQTVVCFPFMKGVIELGTTDHVLEDLSLIQQIKTSLSNSLSVDDPINARATLNSRNNEGVACVAFDDNDYDVELIPEVGYDIINTATSPNGSSNALQANQLPNETFMVESWQVLDDDLSNCVHNSVNSSDCISQNISSAPKVGEDCNNNQKMSLVDPLSEDWHYQKILSELLKSNDQLTMEMHFQNFHPESSFCVWNKGVPVNFQRPRQGTSQKLLKKILVEVPRMHTDGLLESQEENDYREGTRLEADEGMNHVLSERRRRAKLNERFLTLRSMVPSNSKVIFIF