AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93191

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93191.t1 MTR_1g029990 93.636 110 7 0 1 110 1 110 1.46e-65 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93191.t1 AT1G44414 62.727 110 32 2 1 110 1 101 2.51e-43 137

Find 35 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGTTCTTCTTCTTTGC+AGG 0.269891 1.3:+15911699 None:intergenic
GTTCCCGTTTGGCGGTGGCC+AGG 0.363736 1.3:+15911834 MS.gene93191:CDS
GTTCGCCAGGTGGTGAAATC+AGG 0.405267 1.3:+15911738 MS.gene93191:CDS
TTCCCTGGCCACCGCCAAAC+GGG 0.406298 1.3:-15911837 None:intergenic
CAGAGGTGGTTCAACGTTGC+CGG 0.408895 1.3:+15911932 MS.gene93191:CDS
CTTCCCTGGCCACCGCCAAA+CGG 0.426962 1.3:-15911838 None:intergenic
TACAGTTGTTCGCCGCAGTA+AGG 0.428003 1.3:-15912011 None:intergenic
CCACCTCTGGACGGCGGTGG+AGG 0.431088 1.3:-15911918 None:intergenic
TACCGACATCGCAGAGCGTC+CGG 0.441695 1.3:-15911951 None:intergenic
CTCTGGACGGCGGTGGAGGA+AGG 0.447982 1.3:-15911914 None:intergenic
ATTTCACCACCTGGCGAACC+TGG 0.448778 1.3:-15911734 None:intergenic
TTCCCGTTTGGCGGTGGCCA+GGG 0.453465 1.3:+15911835 MS.gene93191:CDS
CGGCAACGTTGAACCACCTC+TGG 0.470731 1.3:-15911931 None:intergenic
TACAAGAGAGTTTCCTTCCC+TGG 0.491912 1.3:-15911852 None:intergenic
TGCAGGAGGGTTAGAAAACC+AGG 0.493973 1.3:+15911716 MS.gene93191:CDS
GTTAGAAAACCAGGTTCGCC+AGG 0.505813 1.3:+15911725 MS.gene93191:CDS
TCTTCGTTCCCGTTTGGCGG+TGG 0.506122 1.3:+15911829 MS.gene93191:CDS
TCTGGACGGCGGTGGAGGAA+GGG 0.511692 1.3:-15911913 None:intergenic
GGAGGAAGGGAATGCGATTG+AGG 0.520352 1.3:-15911900 None:intergenic
CGTTTGGCGGTGGCCAGGGA+AGG 0.537051 1.3:+15911839 MS.gene93191:CDS
ATGTTCTTCTTCTTTGCAGG+AGG 0.539886 1.3:+15911702 MS.gene93191:CDS
GAACCACCTCTGGACGGCGG+TGG 0.545613 1.3:-15911921 None:intergenic
CCTCCACCGCCGTCCAGAGG+TGG 0.545863 1.3:+15911918 MS.gene93191:CDS
CTTCCTCCACCGCCGTCCAG+AGG 0.547039 1.3:+15911915 MS.gene93191:CDS
TGCCGGACGCTCTGCGATGT+CGG 0.549865 1.3:+15911949 MS.gene93191:CDS
TGTTCTTCTTCTTTGCAGGA+GGG 0.566427 1.3:+15911703 MS.gene93191:CDS
CCACACCTGATTTCACCACC+TGG 0.568190 1.3:-15911743 None:intergenic
AACGTTGAACCACCTCTGGA+CGG 0.600945 1.3:-15911927 None:intergenic
CCAGGTGGTGAAATCAGGTG+TGG 0.601448 1.3:+15911743 MS.gene93191:CDS
CAGGTGGTGAAATCAGGTGT+GGG 0.626180 1.3:+15911744 MS.gene93191:CDS
TGGTGAAATCAGGTGTGGGA+AGG 0.629446 1.3:+15911748 MS.gene93191:CDS
AGAAAACCAGGTTCGCCAGG+TGG 0.662890 1.3:+15911728 MS.gene93191:CDS
TTTACGTTCTGTCCTTACTG+CGG 0.664182 1.3:+15911999 MS.gene93191:CDS
GTTGAACCACCTCTGGACGG+CGG 0.698643 1.3:-15911924 None:intergenic
TCGGTACTGTGATCAGAATG+TGG 0.700649 1.3:+15911968 MS.gene93191:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGTTCTTCTTCTTTGCAGG+AGG + chr1.3:15911702-15911721 MS.gene93191:CDS 40.0%
TGTTCTTCTTCTTTGCAGGA+GGG + chr1.3:15911703-15911722 MS.gene93191:CDS 40.0%
TTTACGTTCTGTCCTTACTG+CGG + chr1.3:15911999-15912018 MS.gene93191:CDS 40.0%
TACAAGAGAGTTTCCTTCCC+TGG - chr1.3:15911855-15911874 None:intergenic 45.0%
TCGGTACTGTGATCAGAATG+TGG + chr1.3:15911968-15911987 MS.gene93191:CDS 45.0%
! AGCTTTTCTTCGTTCCCGTT+TGG + chr1.3:15911823-15911842 MS.gene93191:CDS 45.0%
! TTTTCTTCGTTCCCGTTTGG+CGG + chr1.3:15911826-15911845 MS.gene93191:CDS 45.0%
AACGTTGAACCACCTCTGGA+CGG - chr1.3:15911930-15911949 None:intergenic 50.0%
CAGGTGGTGAAATCAGGTGT+GGG + chr1.3:15911744-15911763 MS.gene93191:CDS 50.0%
GTTAGAAAACCAGGTTCGCC+AGG + chr1.3:15911725-15911744 MS.gene93191:CDS 50.0%
TACAGTTGTTCGCCGCAGTA+AGG - chr1.3:15912014-15912033 None:intergenic 50.0%
TGCAGGAGGGTTAGAAAACC+AGG + chr1.3:15911716-15911735 MS.gene93191:CDS 50.0%
TGGTGAAATCAGGTGTGGGA+AGG + chr1.3:15911748-15911767 MS.gene93191:CDS 50.0%
AGAAAACCAGGTTCGCCAGG+TGG + chr1.3:15911728-15911747 MS.gene93191:CDS 55.0%
ATTTCACCACCTGGCGAACC+TGG - chr1.3:15911737-15911756 None:intergenic 55.0%
CAGAGGTGGTTCAACGTTGC+CGG + chr1.3:15911932-15911951 MS.gene93191:CDS 55.0%
CCACACCTGATTTCACCACC+TGG - chr1.3:15911746-15911765 None:intergenic 55.0%
CCAGGTGGTGAAATCAGGTG+TGG + chr1.3:15911743-15911762 MS.gene93191:CDS 55.0%
GGAGGAAGGGAATGCGATTG+AGG - chr1.3:15911903-15911922 None:intergenic 55.0%
GTTCGCCAGGTGGTGAAATC+AGG + chr1.3:15911738-15911757 MS.gene93191:CDS 55.0%
CGGCAACGTTGAACCACCTC+TGG - chr1.3:15911934-15911953 None:intergenic 60.0%
GTTGAACCACCTCTGGACGG+CGG - chr1.3:15911927-15911946 None:intergenic 60.0%
TCTTCGTTCCCGTTTGGCGG+TGG + chr1.3:15911829-15911848 MS.gene93191:CDS 60.0%
! TACCGACATCGCAGAGCGTC+CGG - chr1.3:15911954-15911973 None:intergenic 60.0%
CTTCCCTGGCCACCGCCAAA+CGG - chr1.3:15911841-15911860 None:intergenic 65.0%
TCTGGACGGCGGTGGAGGAA+GGG - chr1.3:15911916-15911935 None:intergenic 65.0%
TGCCGGACGCTCTGCGATGT+CGG + chr1.3:15911949-15911968 MS.gene93191:CDS 65.0%
TTCCCGTTTGGCGGTGGCCA+GGG + chr1.3:15911835-15911854 MS.gene93191:CDS 65.0%
TTCCCTGGCCACCGCCAAAC+GGG - chr1.3:15911840-15911859 None:intergenic 65.0%
CGTTTGGCGGTGGCCAGGGA+AGG + chr1.3:15911839-15911858 MS.gene93191:CDS 70.0%
CTCTGGACGGCGGTGGAGGA+AGG - chr1.3:15911917-15911936 None:intergenic 70.0%
CTTCCTCCACCGCCGTCCAG+AGG + chr1.3:15911915-15911934 MS.gene93191:CDS 70.0%
GAACCACCTCTGGACGGCGG+TGG - chr1.3:15911924-15911943 None:intergenic 70.0%
GTTCCCGTTTGGCGGTGGCC+AGG + chr1.3:15911834-15911853 MS.gene93191:CDS 70.0%
CCACCTCTGGACGGCGGTGG+AGG - chr1.3:15911921-15911940 None:intergenic 75.0%
CCTCCACCGCCGTCCAGAGG+TGG + chr1.3:15911918-15911937 MS.gene93191:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 15911702 15912034 15911702 ID=MS.gene93191
chr1.3 mRNA 15911702 15912034 15911702 ID=MS.gene93191.t1;Parent=MS.gene93191
chr1.3 exon 15911702 15912034 15911702 ID=MS.gene93191.t1.exon1;Parent=MS.gene93191.t1
chr1.3 CDS 15911702 15912034 15911702 ID=cds.MS.gene93191.t1;Parent=MS.gene93191.t1
Gene Sequence

>MS.gene93191

ATGTTCTTCTTCTTTGCAGGAGGGTTAGAAAACCAGGTTCGCCAGGTGGTGAAATCAGGTGTGGGAAGGTGCATCAACTGCAATTCACGCGCTGATCTTGTTGAATACGATAAAGTTCTGAAGCTTTTCTTCGTTCCCGTTTGGCGGTGGCCAGGGAAGGAAACTCTCTTGTATTGTCAAGACTGCAAGTTCATGTTTCCTCAATCGCATTCCCTTCCTCCACCGCCGTCCAGAGGTGGTTCAACGTTGCCGGACGCTCTGCGATGTCGGTACTGTGATCAGAATGTGGCGACTGATTTTACGTTCTGTCCTTACTGCGGCGAACAACTGTAA
Protein sequence

>MS.gene93191.t1

MFFFFAGGLENQVRQVVKSGVGRCINCNSRADLVEYDKVLKLFFVPVWRWPGKETLLYCQDCKFMFPQSHSLPPPPSRGGSTLPDALRCRYCDQNVATDFTFCPYCGEQL