AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93276

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93276.t1 MTR_8g070075 83.508 764 77 5 1 721 1 758 0.0 1280
MS.gene93276.t1 MTR_8g070070 70.026 784 158 12 4 725 1 769 0.0 1038
MS.gene93276.t1 MTR_8g071030 63.818 749 203 10 14 725 32 749 0.0 910
MS.gene93276.t1 MTR_1g035420 54.886 614 193 9 145 724 95 658 0.0 645
MS.gene93276.t1 MTR_1g035400 51.613 558 201 8 186 716 53 568 0.0 538
MS.gene93276.t1 MTR_6g044690 48.816 549 204 11 199 713 59 564 8.52e-167 492
MS.gene93276.t1 MTR_0376s0030 50.000 496 196 8 267 724 96 577 3.24e-162 480
MS.gene93276.t1 MTR_6g043960 44.404 545 207 12 202 713 1 482 2.23e-140 421
MS.gene93276.t1 MTR_2g054460 42.077 549 192 12 199 713 8 464 2.29e-123 376
MS.gene93276.t1 MTR_7g088370 41.379 464 235 13 273 713 231 680 4.79e-104 334
MS.gene93276.t1 MTR_3g011440 39.703 471 237 13 278 712 174 633 1.41e-100 323
MS.gene93276.t1 MTR_5g013420 39.525 463 232 10 278 711 615 1058 1.27e-99 331
MS.gene93276.t1 MTR_5g013420 39.525 463 232 10 278 711 615 1058 1.48e-99 331
MS.gene93276.t1 MTR_8g042750 40.402 448 226 14 298 713 220 658 3.96e-98 318
MS.gene93276.t1 MTR_4g010830 39.520 458 232 10 281 711 510 949 1.20e-94 315
MS.gene93276.t1 MTR_4g010550 39.013 446 221 12 281 711 24 433 3.22e-88 285
MS.gene93276.t1 MTR_7g084090 37.320 485 251 14 272 712 252 727 8.67e-87 289
MS.gene93276.t1 MTR_6g061270 37.076 472 244 15 284 712 203 664 2.30e-83 278
MS.gene93276.t1 MTR_6g061200 37.584 447 247 15 284 712 203 635 1.40e-79 268
MS.gene93276.t1 MTR_7g084090 39.583 384 210 12 272 642 252 626 4.50e-73 250
MS.gene93276.t1 MTR_1g048950 34.868 456 259 14 278 711 68 507 4.80e-57 204
MS.gene93276.t1 MTR_7g450730 31.320 447 212 14 281 711 107 474 1.34e-51 187
MS.gene93276.t1 MTR_7g445710 31.092 476 210 18 281 711 110 512 3.92e-50 184
MS.gene93276.t1 MTR_7g445530 30.882 476 211 18 281 711 110 512 4.74e-50 183
MS.gene93276.t1 MTR_7g052480 31.699 306 174 7 440 712 223 526 9.42e-39 152
MS.gene93276.t1 MTR_7g445600 38.400 125 65 1 587 711 243 355 3.81e-22 99.4
MS.gene93276.t1 MTR_7g445600 41.860 129 61 5 281 409 106 220 3.67e-15 78.2
MS.gene93276.t1 MTR_6g044000 67.925 53 17 0 661 713 117 169 4.31e-19 85.9
MS.gene93276.t1 MTR_6g044000 34.899 149 68 2 202 350 1 120 4.02e-18 82.8
MS.gene93276.t1 MTR_5g018850 32.759 174 102 5 553 711 1310 1483 2.14e-18 90.9
MS.gene93276.t1 MTR_8g078490 31.609 174 104 4 553 711 1056 1229 6.20e-18 89.4
MS.gene93276.t1 MTR_8g078505 26.769 325 176 12 435 711 205 515 1.92e-17 86.7
MS.gene93276.t1 MTR_4g132610 31.034 174 103 4 554 711 851 1023 2.70e-17 87.0
MS.gene93276.t1 MTR_1g069570 23.891 293 154 9 488 713 430 720 3.18e-17 86.7
MS.gene93276.t1 MTR_5g098680 36.076 158 87 4 306 458 267 415 1.16e-16 84.7
MS.gene93276.t1 MTR_8g102580 35.443 158 88 4 306 458 261 409 4.36e-16 82.8
MS.gene93276.t1 MTR_7g445810 37.037 162 79 8 281 437 123 266 4.22e-13 70.9
MS.gene93276.t1 MTR_3g114910 32.680 153 89 4 306 458 187 325 2.51e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93276.t1 AT4G13460 52.456 570 196 9 183 724 127 649 0.0 577
MS.gene93276.t1 AT4G13460 52.456 570 196 9 183 724 127 649 0.0 577
MS.gene93276.t1 AT2G33290 47.320 653 261 12 106 724 47 650 0.0 563
MS.gene93276.t1 AT2G33290 47.320 653 261 12 106 724 47 650 0.0 563
MS.gene93276.t1 AT2G33290 47.320 653 261 12 106 724 47 650 0.0 563
MS.gene93276.t1 AT2G33290 47.320 653 261 12 106 724 47 650 0.0 563
MS.gene93276.t1 AT1G73100 40.000 445 229 10 298 713 204 639 1.63e-96 313
MS.gene93276.t1 AT5G04940 39.238 446 229 11 298 713 207 640 2.61e-92 302
MS.gene93276.t1 AT5G04940 39.238 446 229 11 298 713 207 640 2.61e-92 302
MS.gene93276.t1 AT2G35160 38.839 448 249 12 271 711 298 727 1.85e-90 299
MS.gene93276.t1 AT2G35160 38.839 448 249 12 271 711 334 763 4.24e-90 299
MS.gene93276.t1 AT2G35160 38.839 448 249 12 271 711 334 763 4.24e-90 299
MS.gene93276.t1 AT2G35160 38.839 448 249 12 271 711 334 763 4.24e-90 299
MS.gene93276.t1 AT2G35160 38.839 448 249 12 271 711 334 763 4.24e-90 299
MS.gene93276.t1 AT2G22740 34.892 556 300 13 193 711 229 759 1.85e-88 295
MS.gene93276.t1 AT2G22740 34.892 556 300 13 193 711 229 759 1.85e-88 295
MS.gene93276.t1 AT5G13960 36.463 458 240 15 298 712 11 460 2.34e-78 259
MS.gene93276.t1 AT5G13960 36.304 460 238 16 298 712 145 594 3.40e-78 263
MS.gene93276.t1 AT2G24740 36.795 443 227 17 299 713 307 724 1.99e-70 245
MS.gene93276.t1 AT1G17770 33.630 449 255 16 298 713 223 661 3.67e-70 243
MS.gene93276.t1 AT5G47150 39.560 182 101 4 277 458 152 324 5.85e-28 115
MS.gene93276.t1 AT5G47160 40.588 170 91 5 286 454 247 407 4.08e-24 106
MS.gene93276.t1 AT5G47160 40.588 170 91 5 286 454 247 407 4.08e-24 106
MS.gene93276.t1 AT5G47160 40.588 170 91 5 286 454 247 407 4.08e-24 106
MS.gene93276.t1 AT2G05900 39.655 174 97 6 343 516 26 191 3.74e-22 98.2
MS.gene93276.t1 AT2G23740 34.807 181 98 6 555 716 1177 1356 1.02e-21 101
MS.gene93276.t1 AT2G23740 34.807 181 98 6 555 716 1184 1363 1.04e-21 101
MS.gene93276.t1 AT2G23740 34.807 181 98 6 555 716 1184 1363 1.04e-21 101
MS.gene93276.t1 AT2G23740 34.807 181 98 6 555 716 1184 1363 1.04e-21 101
MS.gene93276.t1 AT1G57820 35.849 159 87 5 306 458 277 426 2.86e-16 83.2
MS.gene93276.t1 AT1G57820 35.849 159 87 5 306 458 277 426 2.86e-16 83.2
MS.gene93276.t1 AT2G23740 36.364 132 67 5 555 670 1184 1314 8.45e-16 82.4
MS.gene93276.t1 AT2G23740 36.364 132 67 5 555 670 1184 1314 8.45e-16 82.4
MS.gene93276.t1 AT1G57820 35.256 156 89 5 306 458 277 423 2.93e-15 80.1
MS.gene93276.t1 AT3G03750 27.899 276 132 13 486 712 66 323 1.40e-14 76.3
MS.gene93276.t1 AT1G66050 33.333 159 91 4 306 458 262 411 3.77e-14 76.6
MS.gene93276.t1 AT1G66040 33.333 159 91 4 306 458 262 411 3.86e-14 76.6
MS.gene93276.t1 AT5G39550 34.591 159 89 5 306 458 262 411 6.06e-14 75.9
MS.gene93276.t1 AT1G76710 30.263 152 96 5 572 719 66 211 2.30e-12 70.5
MS.gene93276.t1 AT1G76710 30.263 152 96 5 572 719 66 211 2.30e-12 70.5
MS.gene93276.t1 AT1G76710 30.263 152 96 5 572 719 66 211 2.41e-12 70.5
MS.gene93276.t1 AT1G76710 30.263 152 96 5 572 719 66 211 2.46e-12 70.5
MS.gene93276.t1 AT4G08590 31.818 154 91 4 306 458 237 377 3.46e-11 66.6

Find 205 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGGGAGATTAAGAAAAT+TGG 0.151275 1.3:+34001358 MS.gene93276:CDS
GCTACTAGTGTGATTGTTTC+AGG 0.189529 1.3:+34000992 MS.gene93276:CDS
TCCTCAAAAGGTTTAGGTTT+TGG 0.199470 1.3:-34000056 None:intergenic
AAGCGGTGCTCCGGTGTAAA+TGG 0.221260 1.3:-34000018 None:intergenic
TAGGTCCTTGGATCTTATTC+AGG 0.256771 1.3:+34001777 MS.gene93276:CDS
AGATTCTTGTTTCTTTGAAA+TGG 0.278142 1.3:-34000393 None:intergenic
GTTAATGATAAATGATCTAT+TGG 0.281493 1.3:-33999909 None:intergenic
AAGCAAACCCTGAAGATTAT+AGG 0.291078 1.3:-34001620 None:intergenic
AAGAAATCCTCAAAAGGTTT+AGG 0.291529 1.3:-34000062 None:intergenic
AATTATTGAGGAGAAGAAAT+TGG 0.298748 1.3:+34000574 MS.gene93276:CDS
AGAGTTTCCCTATAATCTTC+AGG 0.299770 1.3:+34001612 MS.gene93276:CDS
GGTAGCTATGGTTAAGAAAA+AGG 0.309499 1.3:+34000424 MS.gene93276:CDS
TATGGTTGCATCGCGATAAA+AGG 0.310327 1.3:+34000828 MS.gene93276:CDS
AGCTTGCCCGCCCACTCGTC+AGG 0.315436 1.3:-34002169 None:intergenic
TGCAACACGATTCCGACAAT+TGG 0.331483 1.3:-34001695 None:intergenic
AGGTGGTTATGAGGATGATA+TGG 0.333296 1.3:+34001012 MS.gene93276:CDS
CAGGTCTGCGCAAACTGGTT+GGG 0.335408 1.3:+34001750 MS.gene93276:CDS
GGATTGAGGGTTGACATGTT+AGG 0.336861 1.3:-33999984 None:intergenic
ATACATTTGGACTCGAACTA+TGG 0.339033 1.3:-34002036 None:intergenic
TTGGGAGGACAAGAGCAAAA+AGG 0.339571 1.3:-34001676 None:intergenic
TGAGAAGGGGAAGTAATAGA+AGG 0.340316 1.3:-33999942 None:intergenic
GGTGTAAATGGGAGTGATTT+GGG 0.344967 1.3:-34000006 None:intergenic
CGCAAACTGGTTGGGGAGTT+AGG 0.346834 1.3:+34001758 MS.gene93276:CDS
CGGTGTAAATGGGAGTGATT+TGG 0.348980 1.3:-34000007 None:intergenic
AGAGGTAGCAACAGGGGTGG+AGG 0.363969 1.3:+34000724 MS.gene93276:intron
TATATAGGATTACTGATTGC+TGG 0.364491 1.3:+34001242 MS.gene93276:CDS
ACCTGATATAGATTATTCTA+TGG 0.365689 1.3:+34001976 MS.gene93276:CDS
AGCAAACCCTGAAGATTATA+GGG 0.370075 1.3:-34001619 None:intergenic
CAAAACGGTTTATAGATAGA+TGG 0.370356 1.3:+34001890 MS.gene93276:intron
ACAAATTGAACAAATACATT+TGG 0.370419 1.3:-34002049 None:intergenic
CCCGGTATACTCGCAGATAA+AGG 0.370510 1.3:-34001806 None:intergenic
ATGTTTATGATGGATTATAT+AGG 0.373190 1.3:+34001227 MS.gene93276:CDS
TCCATAGAATAATCTATATC+AGG 0.373576 1.3:-34001977 None:intergenic
TCAACATTGCGAATTGTTGC+TGG 0.380006 1.3:+34002004 MS.gene93276:CDS
GATTGAGGGTTGACATGTTA+GGG 0.381292 1.3:-33999983 None:intergenic
ATGGTGGAATTATGTGTAAT+TGG 0.382160 1.3:+34000899 MS.gene93276:CDS
TATAGATAGATGGGCAGAAT+GGG 0.382516 1.3:+34001900 MS.gene93276:intron
ATGTCATTAAATAGCCTAAC+AGG 0.384989 1.3:-34001442 None:intergenic
CTAGGGAGATTAAGAAAATT+GGG 0.390286 1.3:+34001359 MS.gene93276:CDS
GATTCTTGTTTCTTTGAAAT+GGG 0.395729 1.3:-34000392 None:intergenic
TGGGAGTGATTTGGGGATTG+AGG 0.396090 1.3:-33999998 None:intergenic
AGCATAGGTTGGAAGTGTTC+AGG 0.397659 1.3:+34001731 MS.gene93276:CDS
ATCATAGTCGTAGTTTCATA+AGG 0.403218 1.3:+34000777 MS.gene93276:CDS
TCGGAATCGTGTTGCACAAA+AGG 0.405257 1.3:+34001702 MS.gene93276:CDS
GCTCTTGTCCTCCCAATTGT+CGG 0.405346 1.3:+34001683 MS.gene93276:CDS
GCATTACGGGATCGAGATTA+GGG 0.408510 1.3:+34001153 MS.gene93276:CDS
GGGAGTGATTTGGGGATTGA+GGG 0.409415 1.3:-33999997 None:intergenic
GGTAAGGTTTATGTTTATGA+TGG 0.410667 1.3:+34001217 MS.gene93276:CDS
CTGATCACAAATTTGAATTG+AGG 0.414878 1.3:-34001092 None:intergenic
CGCGATGCAACCATAACTTT+CGG 0.415089 1.3:-34000820 None:intergenic
AACATGGAAGAGGTAGCAAC+AGG 0.418367 1.3:+34000716 MS.gene93276:intron
CATCGCGATAAAAGGATAGT+TGG 0.419077 1.3:+34000836 MS.gene93276:CDS
GCTTGTGAAAGGGAAGCCTT+TGG 0.419411 1.3:+34001639 MS.gene93276:CDS
AAAATGATAAAGGGATTTAT+CGG 0.421885 1.3:-34000234 None:intergenic
TGCATTACGGGATCGAGATT+AGG 0.423214 1.3:+34001152 MS.gene93276:CDS
GGCAGTGAAGAGATTGGTTG+AGG 0.423218 1.3:+34000616 MS.gene93276:CDS
TTATGAGGATGATATGGAAT+TGG 0.423427 1.3:+34001018 MS.gene93276:CDS
TAAATGATCTATTGGATTCA+TGG 0.427471 1.3:-33999901 None:intergenic
AGAGGGTGGAAACCTTGCTT+TGG 0.427528 1.3:+34001120 MS.gene93276:CDS
GGTAGCAACAGGGGTGGAGG+GGG 0.427970 1.3:+34000727 MS.gene93276:intron
GGTGAGAAGAAGTTTGAGAA+GGG 0.429309 1.3:-33999956 None:intergenic
TGAAGCGGCAAAAGCATTGT+TGG 0.430143 1.3:-34000273 None:intergenic
GGGTTGACATGTTAGGGATT+TGG 0.431533 1.3:-33999977 None:intergenic
AATTGAGGGGCAAGCTAAGA+TGG 0.435322 1.3:+34001315 MS.gene93276:CDS
TATAAGTTATTGAGAATTGA+GGG 0.436671 1.3:+34001301 MS.gene93276:CDS
CGGAATCGTGTTGCACAAAA+GGG 0.437967 1.3:+34001703 MS.gene93276:CDS
GCCTTTATCTGCGAGTATAC+CGG 0.438710 1.3:+34001805 MS.gene93276:CDS
TCCTTGGATCTTATTCAGGC+TGG 0.446886 1.3:+34001781 MS.gene93276:CDS
AGGTAGCAACAGGGGTGGAG+GGG 0.448282 1.3:+34000726 MS.gene93276:intron
CCTTTATCTGCGAGTATACC+GGG 0.450373 1.3:+34001806 MS.gene93276:CDS
CATCAGAGCGGGAAAGCTAC+TGG 0.454438 1.3:+34001532 MS.gene93276:CDS
AGAGAACTCAGCATAGACTA+TGG 0.455539 1.3:+34002142 MS.gene93276:CDS
TTGGAGCGTAGTATGCATTA+CGG 0.455982 1.3:+34001139 MS.gene93276:CDS
TGGTTTGAAGAAGGGATGTC+TGG 0.461289 1.3:+34001262 MS.gene93276:CDS
TCAGGTATTAATTGATATGA+TGG 0.462483 1.3:-34001959 None:intergenic
GAGTTTCCCTATAATCTTCA+GGG 0.462595 1.3:+34001613 MS.gene93276:CDS
AAGTAATAGAAGGGGTTTCA+AGG 0.463004 1.3:-33999932 None:intergenic
GATTGTTTCAGGTGGTTATG+AGG 0.465005 1.3:+34001003 MS.gene93276:CDS
AATCAACCTCATTTCCCTCC+TGG 0.471602 1.3:+34000140 MS.gene93276:CDS
ACAATCACACTAGTAGCAAT+TGG 0.471909 1.3:-34000986 None:intergenic
ACTGATTGCTGGTTTGAAGA+AGG 0.473748 1.3:+34001253 MS.gene93276:CDS
AGAGGGAGAATGTTCCTGTT+AGG 0.476652 1.3:+34001428 MS.gene93276:CDS
GAGAAGGGGAAGTAATAGAA+GGG 0.479390 1.3:-33999941 None:intergenic
TCAGGTCTGCGCAAACTGGT+TGG 0.479909 1.3:+34001749 MS.gene93276:CDS
AGTAGCAATTGGCTCGCCAT+TGG 0.481657 1.3:-34000975 None:intergenic
TAATGAAGGACCGAAAGTTA+TGG 0.481674 1.3:+34000810 MS.gene93276:CDS
CTTCAGGGTTTGCTTGTGAA+AGG 0.482699 1.3:+34001628 MS.gene93276:CDS
TCAGTCAATATTTCCGACCT+TGG 0.482765 1.3:+34000305 MS.gene93276:CDS
AGGTCTGCGCAAACTGGTTG+GGG 0.482929 1.3:+34001751 MS.gene93276:CDS
AATTCTTCTTTCTTCCTCCA+TGG 0.483343 1.3:-34001069 None:intergenic
TGGAGCGTAGTATGCATTAC+GGG 0.484208 1.3:+34001140 MS.gene93276:CDS
GCAACACGATTCCGACAATT+GGG 0.485278 1.3:-34001694 None:intergenic
GTATAAGTTATTGAGAATTG+AGG 0.488542 1.3:+34001300 MS.gene93276:CDS
GTGTTCAGGTCTGCGCAAAC+TGG 0.491340 1.3:+34001745 MS.gene93276:CDS
GGATGAGAAAAGAATTATTG+AGG 0.492648 1.3:+34000562 MS.gene93276:CDS
GGAGATTAAGAAAATTGGGT+TGG 0.494139 1.3:+34001363 MS.gene93276:CDS
TGTGGAGGCAGTGAAGAGAT+TGG 0.494725 1.3:+34000610 MS.gene93276:CDS
AGCGGTGCTCCGGTGTAAAT+GGG 0.497581 1.3:-34000017 None:intergenic
AAACAACCTCATTTCCCTCA+TGG 0.500206 1.3:+34000104 MS.gene93276:CDS
ATTGGTTGAGGAGAATATGT+TGG 0.501697 1.3:+34000628 MS.gene93276:CDS
GGATTTATCGGAAGTAGCAT+TGG 0.502335 1.3:-34000222 None:intergenic
TTCAGGGTTTGCTTGTGAAA+GGG 0.502624 1.3:+34001629 MS.gene93276:CDS
TATGGTTTGAAGCGGTGCTC+CGG 0.502918 1.3:-34000027 None:intergenic
CTTCTTTCTTCCTCCATGGC+CGG 0.504486 1.3:-34001065 None:intergenic
CTGATTGCTGGTTTGAAGAA+GGG 0.504895 1.3:+34001254 MS.gene93276:CDS
GGAAGATACTCATAATAAAG+AGG 0.506268 1.3:-34001481 None:intergenic
TGTGTGGAGGCTTGTACTGA+TGG 0.507598 1.3:+34001562 MS.gene93276:CDS
AGAATATGTTGGATGAGGAT+AGG 0.509832 1.3:+34000639 MS.gene93276:CDS
GAGGTAGCAACAGGGGTGGA+GGG 0.511415 1.3:+34000725 MS.gene93276:intron
TGGTGAGAAGAAGTTTGAGA+AGG 0.516679 1.3:-33999957 None:intergenic
ACTAAATCAGAACCCTATCA+TGG 0.518796 1.3:+34000328 MS.gene93276:CDS
GGCATCCCTGACGAGTGGGC+GGG 0.520972 1.3:+34002163 MS.gene93276:CDS
AGATAGATGGGCAGAATGGG+GGG 0.522533 1.3:+34001903 MS.gene93276:intron
ATCTTGTTCGACGGTAGCTA+TGG 0.523651 1.3:+34000412 MS.gene93276:CDS
GAGGATTTCTTCACCACCCA+AGG 0.525934 1.3:+34000074 MS.gene93276:CDS
TCATAGTCGTAGTTTCATAA+GGG 0.527387 1.3:+34000778 MS.gene93276:CDS
TAGATAGATGGGCAGAATGG+GGG 0.530366 1.3:+34001902 MS.gene93276:intron
TTATAGATAGATGGGCAGAA+TGG 0.533260 1.3:+34001899 MS.gene93276:intron
GTAATAATCAATACCGGCCA+TGG 0.534042 1.3:+34001052 MS.gene93276:CDS
GTGAGAAGAAGTTTGAGAAG+GGG 0.534971 1.3:-33999955 None:intergenic
AGTAATAGAAGGGGTTTCAA+GGG 0.536332 1.3:-33999931 None:intergenic
AAAACGGTTTATAGATAGAT+GGG 0.536629 1.3:+34001891 MS.gene93276:intron
TTATGATTCGCTACGTGTGT+TGG 0.545706 1.3:+34000529 MS.gene93276:CDS
GTGGTGAAGAAATCCTCAAA+AGG 0.546407 1.3:-34000068 None:intergenic
AATTTGTGATCAGAAATTAG+AGG 0.546612 1.3:+34001102 MS.gene93276:CDS
GACTGAGTTCTCCATTGAAG+CGG 0.547564 1.3:-34000288 None:intergenic
AAAGGGGATCAAGCATAGGT+TGG 0.547811 1.3:+34001720 MS.gene93276:CDS
GGTTTGTGTTTCATCAGAGC+GGG 0.550167 1.3:+34001521 MS.gene93276:CDS
TTGATACCTGGAGTTTATGT+TGG 0.550374 1.3:+34000860 MS.gene93276:CDS
AGAAGGGGAAGTAATAGAAG+GGG 0.554124 1.3:-33999940 None:intergenic
TAGGGTTCTGATTTAGTCCA+AGG 0.558060 1.3:-34000322 None:intergenic
CCGTCTCATGCTATATGCAA+TGG 0.560658 1.3:+34002102 MS.gene93276:CDS
GAAGTAGCATTGGAAGGTTG+TGG 0.561967 1.3:-34000212 None:intergenic
TGTTGATATTTCACACAAGA+GGG 0.562610 1.3:+34001411 MS.gene93276:CDS
AGGCAAGTCAGATGCTTGTG+AGG 0.562686 1.3:+34000444 MS.gene93276:CDS
GGTGATGTAATAATCAATAC+CGG 0.563689 1.3:+34001046 MS.gene93276:CDS
CTAAATCAGAACCCTATCAT+GGG 0.565746 1.3:+34000329 MS.gene93276:CDS
GGGTGCGTCTAGCTCTGGTA+AGG 0.568302 1.3:+34001201 MS.gene93276:CDS
TGGCATCCCTGACGAGTGGG+CGG 0.569853 1.3:+34002162 MS.gene93276:CDS
CTGTTGATATTTCACACAAG+AGG 0.573952 1.3:+34001410 MS.gene93276:CDS
CATTGCATATAGCATGAGAC+GGG 0.574005 1.3:-34002101 None:intergenic
TTATCGGAAGTAGCATTGGA+AGG 0.574777 1.3:-34000218 None:intergenic
TTCGTGGGATGAAGTATGAA+GGG 0.576304 1.3:+34001179 MS.gene93276:CDS
ATAGATAGATGGGCAGAATG+GGG 0.576328 1.3:+34001901 MS.gene93276:intron
AAGGGCTGCGAGGTTAATGA+AGG 0.576510 1.3:+34000796 MS.gene93276:CDS
ATCGAGATTAGGGTCATTCG+TGG 0.577854 1.3:+34001163 MS.gene93276:CDS
GGTTTCAGATCTGAGTATGA+AGG 0.579176 1.3:+34000466 MS.gene93276:CDS
ATTTGTGATCAGAAATTAGA+GGG 0.579266 1.3:+34001103 MS.gene93276:CDS
AGGATAGTTGGATTGATACC+TGG 0.579821 1.3:+34000848 MS.gene93276:CDS
ATTCGTGGGATGAAGTATGA+AGG 0.581363 1.3:+34001178 MS.gene93276:CDS
GCTTGCCCGCCCACTCGTCA+GGG 0.582498 1.3:-34002168 None:intergenic
GCATACTACGCTCCAAAGCA+AGG 0.582921 1.3:-34001132 None:intergenic
TGAGGAGAATATGTTGGATG+AGG 0.583666 1.3:+34000634 MS.gene93276:CDS
TTGCATATGCAGATACAAGC+TGG 0.583868 1.3:+34000923 MS.gene93276:CDS
TGCTGAGTTCTCTCAATGGA+GGG 0.584149 1.3:-34002132 None:intergenic
GTGTAAATGGGAGTGATTTG+GGG 0.585866 1.3:-34000005 None:intergenic
ATTGAGGGGCAAGCTAAGAT+GGG 0.587188 1.3:+34001316 MS.gene93276:CDS
ACATCGCCAACATAAACTCC+AGG 0.587833 1.3:-34000866 None:intergenic
CCATTGCATATAGCATGAGA+CGG 0.588288 1.3:-34002102 None:intergenic
TCATATCCAGGAGGGAAATG+AGG 0.588395 1.3:-34000146 None:intergenic
GGGACGAGTATGGTTTGAAG+CGG 0.590830 1.3:-34000035 None:intergenic
TGGTTGGGGAGTTAGGTCCT+TGG 0.593647 1.3:+34001765 MS.gene93276:CDS
CTCTGATGAAACACAAACCA+CGG 0.594028 1.3:-34001517 None:intergenic
GATAGATGGGCAGAATGGGG+GGG 0.596452 1.3:+34001904 MS.gene93276:intron
TCATATCCATGAGGGAAATG+AGG 0.597266 1.3:-34000110 None:intergenic
AGCTACTGGTTGTGAATGTG+TGG 0.598292 1.3:+34001546 MS.gene93276:CDS
GGCAAGTCAGATGCTTGTGA+GGG 0.598305 1.3:+34000445 MS.gene93276:CDS
ACTAGTGTGATTGTTTCAGG+TGG 0.600870 1.3:+34000995 MS.gene93276:CDS
TCAACAGAAAGACAATACAT+AGG 0.601273 1.3:-34001394 None:intergenic
TGTTGTAGTACTCAGTGTAG+AGG 0.601469 1.3:-34000187 None:intergenic
ATGCTGAGTTCTCTCAATGG+AGG 0.603033 1.3:-34002133 None:intergenic
TCTATGCTGAGTTCTCTCAA+TGG 0.607917 1.3:-34002136 None:intergenic
AGATGCATCGCAACATGATG+AGG 0.612112 1.3:+34000492 MS.gene93276:CDS
GGAAGAGGTAGCAACAGGGG+TGG 0.616106 1.3:+34000721 MS.gene93276:intron
GTTCTGATTTAGTCCAAGGT+CGG 0.618069 1.3:-34000318 None:intergenic
TCCAGCCTGAATAAGATCCA+AGG 0.619090 1.3:-34001782 None:intergenic
GAAACAAGAATCTTGTTCGA+CGG 0.620737 1.3:+34000403 MS.gene93276:CDS
CTATGGCATCCCTGACGAGT+GGG 0.626017 1.3:+34002159 MS.gene93276:CDS
GCAACATGATGAGGAGAACA+AGG 0.627298 1.3:+34000501 MS.gene93276:CDS
ACATGGAAGAGGTAGCAACA+GGG 0.627473 1.3:+34000717 MS.gene93276:intron
ACGTGTGTTGGAAAGTATTG+AGG 0.633669 1.3:+34000541 MS.gene93276:CDS
TGGTTTGTGTTTCATCAGAG+CGG 0.634243 1.3:+34001520 MS.gene93276:CDS
TGTGATCAGAAATTAGAGGG+TGG 0.640509 1.3:+34001106 MS.gene93276:CDS
CACAAAAGGGGATCAAGCAT+AGG 0.642092 1.3:+34001716 MS.gene93276:CDS
TACTGGTTGTGAATGTGTGG+AGG 0.644458 1.3:+34001549 MS.gene93276:CDS
ATAAGTTATTGAGAATTGAG+GGG 0.645000 1.3:+34001302 MS.gene93276:CDS
CATGGAAGAGGTAGCAACAG+GGG 0.645322 1.3:+34000718 MS.gene93276:intron
CAAATCACTCCCATTTACAC+CGG 0.645622 1.3:+34000008 MS.gene93276:CDS
TAAATCAGAACCCTATCATG+GGG 0.649974 1.3:+34000330 MS.gene93276:CDS
ACTATGGCATCCCTGACGAG+TGG 0.651275 1.3:+34002158 MS.gene93276:CDS
ACACGATTCCGACAATTGGG+AGG 0.651351 1.3:-34001691 None:intergenic
TCGTGGGATGAAGTATGAAG+GGG 0.657054 1.3:+34001180 MS.gene93276:CDS
CCTAAAAGTAGGTCTTCCAA+TGG 0.661785 1.3:+34000959 MS.gene93276:CDS
GGAATCGTGTTGCACAAAAG+GGG 0.665750 1.3:+34001704 MS.gene93276:CDS
TCGAGATTAGGGTCATTCGT+GGG 0.665810 1.3:+34001164 MS.gene93276:CDS
TGTTGTGTTCATATCCATGA+GGG 0.680837 1.3:-34000118 None:intergenic
CGTGGGATGAAGTATGAAGG+GGG 0.704890 1.3:+34001181 MS.gene93276:CDS
CTTTATCTGCGAGTATACCG+GGG 0.705407 1.3:+34001807 MS.gene93276:CDS
GATGATATGGAATTGGACGA+TGG 0.705518 1.3:+34001025 MS.gene93276:CDS
ATAATCAATACCGGCCATGG+AGG 0.709610 1.3:+34001055 MS.gene93276:CDS
ATTGCATATAGCATGAGACG+GGG 0.721884 1.3:-34002100 None:intergenic
TTGCATATAGCATGAGACGG+GGG 0.722787 1.3:-34002099 None:intergenic
GTAGTTTCATAAGGGCTGCG+AGG 0.729374 1.3:+34000786 MS.gene93276:CDS
TTGTTGTGTTCATATCCATG+AGG 0.736385 1.3:-34000119 None:intergenic
AAATCAGAACCCTATCATGG+GGG 0.761643 1.3:+34000331 MS.gene93276:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTGAGAAAATGATAAA+GGG - chr1.3:34000246-34000265 None:intergenic 15.0%
!! AAAATGATAAAGGGATTTAT+CGG - chr1.3:34000237-34000256 None:intergenic 20.0%
!! ACAAATTGAACAAATACATT+TGG - chr1.3:34002052-34002071 None:intergenic 20.0%
!! GTTAATGATAAATGATCTAT+TGG - chr1.3:33999912-33999931 None:intergenic 20.0%
!! TATAAGTTATTGAGAATTGA+GGG + chr1.3:34001301-34001320 MS.gene93276:CDS 20.0%
!!! ATGTTTATGATGGATTATAT+AGG + chr1.3:34001227-34001246 MS.gene93276:CDS 20.0%
!!! CAATTTTGAGAAAATGATAA+AGG - chr1.3:34000247-34000266 None:intergenic 20.0%
! AAAACGGTTTATAGATAGAT+GGG + chr1.3:34001891-34001910 MS.gene93276:intron 25.0%
! AATTATTGAGGAGAAGAAAT+TGG + chr1.3:34000574-34000593 MS.gene93276:CDS 25.0%
! AATTTGTGATCAGAAATTAG+AGG + chr1.3:34001102-34001121 MS.gene93276:CDS 25.0%
! ACCTGATATAGATTATTCTA+TGG + chr1.3:34001976-34001995 MS.gene93276:CDS 25.0%
! ACTCGTTAATATATCCAAAA+CGG + chr1.3:34001875-34001894 MS.gene93276:intron 25.0%
! ATAAGTTATTGAGAATTGAG+GGG + chr1.3:34001302-34001321 MS.gene93276:CDS 25.0%
! ATTTGTGATCAGAAATTAGA+GGG + chr1.3:34001103-34001122 MS.gene93276:CDS 25.0%
! GTATAAGTTATTGAGAATTG+AGG + chr1.3:34001300-34001319 MS.gene93276:CDS 25.0%
! TCAGGTATTAATTGATATGA+TGG - chr1.3:34001962-34001981 None:intergenic 25.0%
! TCCATAGAATAATCTATATC+AGG - chr1.3:34001980-34001999 None:intergenic 25.0%
!! AGATTCTTGTTTCTTTGAAA+TGG - chr1.3:34000396-34000415 None:intergenic 25.0%
!! TTGATTTTCTTCCTAAAAGT+AGG + chr1.3:34000948-34000967 MS.gene93276:CDS 25.0%
!!! GATTCTTGTTTCTTTGAAAT+GGG - chr1.3:34000395-34000414 None:intergenic 25.0%
!!! GCTCAAATTTTAACAATGAA+TGG + chr1.3:34001850-34001869 MS.gene93276:intron 25.0%
AAGAAATCCTCAAAAGGTTT+AGG - chr1.3:34000065-34000084 None:intergenic 30.0%
ATCATAGTCGTAGTTTCATA+AGG + chr1.3:34000777-34000796 MS.gene93276:CDS 30.0%
ATGTCATTAAATAGCCTAAC+AGG - chr1.3:34001445-34001464 None:intergenic 30.0%
CAAAACGGTTTATAGATAGA+TGG + chr1.3:34001890-34001909 MS.gene93276:intron 30.0%
CTAGGGAGATTAAGAAAATT+GGG + chr1.3:34001359-34001378 MS.gene93276:CDS 30.0%
CTGATCACAAATTTGAATTG+AGG - chr1.3:34001095-34001114 None:intergenic 30.0%
GGAAGATACTCATAATAAAG+AGG - chr1.3:34001484-34001503 None:intergenic 30.0%
GGATGAGAAAAGAATTATTG+AGG + chr1.3:34000562-34000581 MS.gene93276:CDS 30.0%
GGTAAGGTTTATGTTTATGA+TGG + chr1.3:34001217-34001236 MS.gene93276:CDS 30.0%
GGTGATGTAATAATCAATAC+CGG + chr1.3:34001046-34001065 MS.gene93276:CDS 30.0%
TCAACAGAAAGACAATACAT+AGG - chr1.3:34001397-34001416 None:intergenic 30.0%
TCATAGTCGTAGTTTCATAA+GGG + chr1.3:34000778-34000797 MS.gene93276:CDS 30.0%
TGTTGATATTTCACACAAGA+GGG + chr1.3:34001411-34001430 MS.gene93276:CDS 30.0%
TTATGAGGATGATATGGAAT+TGG + chr1.3:34001018-34001037 MS.gene93276:CDS 30.0%
! ATGGTGGAATTATGTGTAAT+TGG + chr1.3:34000899-34000918 MS.gene93276:CDS 30.0%
! CATCTATCTATAAACCGTTT+TGG - chr1.3:34001892-34001911 None:intergenic 30.0%
! TATATAGGATTACTGATTGC+TGG + chr1.3:34001242-34001261 MS.gene93276:CDS 30.0%
! TGATTTTTGTGTTCATATCC+AGG - chr1.3:34000161-34000180 None:intergenic 30.0%
!! TGTTGTTCTAAAAAAGGCTA+GGG + chr1.3:34001342-34001361 MS.gene93276:CDS 30.0%
AAGCAAACCCTGAAGATTAT+AGG - chr1.3:34001623-34001642 None:intergenic 35.0%
AAGTAATAGAAGGGGTTTCA+AGG - chr1.3:33999935-33999954 None:intergenic 35.0%
AATTCTTCTTTCTTCCTCCA+TGG - chr1.3:34001072-34001091 None:intergenic 35.0%
ACAATCACACTAGTAGCAAT+TGG - chr1.3:34000989-34001008 None:intergenic 35.0%
ACTAAATCAGAACCCTATCA+TGG + chr1.3:34000328-34000347 MS.gene93276:CDS 35.0%
AGAATATGTTGGATGAGGAT+AGG + chr1.3:34000639-34000658 MS.gene93276:CDS 35.0%
AGAGTTTCCCTATAATCTTC+AGG + chr1.3:34001612-34001631 MS.gene93276:CDS 35.0%
AGCAAACCCTGAAGATTATA+GGG - chr1.3:34001622-34001641 None:intergenic 35.0%
AGTAATAGAAGGGGTTTCAA+GGG - chr1.3:33999934-33999953 None:intergenic 35.0%
ATACATTTGGACTCGAACTA+TGG - chr1.3:34002039-34002058 None:intergenic 35.0%
ATTGGTTGAGGAGAATATGT+TGG + chr1.3:34000628-34000647 MS.gene93276:CDS 35.0%
CTAAATCAGAACCCTATCAT+GGG + chr1.3:34000329-34000348 MS.gene93276:CDS 35.0%
CTGTTGATATTTCACACAAG+AGG + chr1.3:34001410-34001429 MS.gene93276:CDS 35.0%
GAAACAAGAATCTTGTTCGA+CGG + chr1.3:34000403-34000422 MS.gene93276:CDS 35.0%
GAGTTTCCCTATAATCTTCA+GGG + chr1.3:34001613-34001632 MS.gene93276:CDS 35.0%
GCTAGGGAGATTAAGAAAAT+TGG + chr1.3:34001358-34001377 MS.gene93276:CDS 35.0%
GGAGATTAAGAAAATTGGGT+TGG + chr1.3:34001363-34001382 MS.gene93276:CDS 35.0%
GGTAGCTATGGTTAAGAAAA+AGG + chr1.3:34000424-34000443 MS.gene93276:CDS 35.0%
TAAATCAGAACCCTATCATG+GGG + chr1.3:34000330-34000349 MS.gene93276:CDS 35.0%
TAATGAAGGACCGAAAGTTA+TGG + chr1.3:34000810-34000829 MS.gene93276:CDS 35.0%
TATAGATAGATGGGCAGAAT+GGG + chr1.3:34001900-34001919 MS.gene93276:intron 35.0%
TGTTGTGTTCATATCCATGA+GGG - chr1.3:34000121-34000140 None:intergenic 35.0%
TTATAGATAGATGGGCAGAA+TGG + chr1.3:34001899-34001918 MS.gene93276:intron 35.0%
TTCCCTTGTTAAAATTACCC+CGG - chr1.3:34001827-34001846 None:intergenic 35.0%
TTGATACCTGGAGTTTATGT+TGG + chr1.3:34000860-34000879 MS.gene93276:CDS 35.0%
TTGTTGTGTTCATATCCATG+AGG - chr1.3:34000122-34000141 None:intergenic 35.0%
! AACCACGGAAAAGTAGTTTT+CGG - chr1.3:34001505-34001524 None:intergenic 35.0%
! ATTGGCTGATGAAGTTTTTG+TGG + chr1.3:34000592-34000611 MS.gene93276:CDS 35.0%
! CGATGTTTTCTTGTATATGG+TGG + chr1.3:34000883-34000902 MS.gene93276:CDS 35.0%
! TGCTTTTGCTCTATGAAGAA+TGG + chr1.3:34001586-34001605 MS.gene93276:CDS 35.0%
! TGGCGATGTTTTCTTGTATA+TGG + chr1.3:34000880-34000899 MS.gene93276:CDS 35.0%
! TTTTGCTCTATGAAGAATGG+AGG + chr1.3:34001589-34001608 MS.gene93276:CDS 35.0%
! TTTTGTGTTCATATCCAGGA+GGG - chr1.3:34000157-34000176 None:intergenic 35.0%
! TTTTTGTGTTCATATCCAGG+AGG - chr1.3:34000158-34000177 None:intergenic 35.0%
!! ATACCGGGGTAATTTTAACA+AGG + chr1.3:34001821-34001840 MS.gene93276:intron 35.0%
!! CCTCAAAAGGTTTAGGTTTT+GGG - chr1.3:34000058-34000077 None:intergenic 35.0%
!! GGGTAGTGTTGTTCTAAAAA+AGG + chr1.3:34001336-34001355 MS.gene93276:CDS 35.0%
!! GTGTTGTTCTAAAAAAGGCT+AGG + chr1.3:34001341-34001360 MS.gene93276:CDS 35.0%
!! TACCGGGGTAATTTTAACAA+GGG + chr1.3:34001822-34001841 MS.gene93276:intron 35.0%
!! TCCTCAAAAGGTTTAGGTTT+TGG - chr1.3:34000059-34000078 None:intergenic 35.0%
!! TGTTTCTGAAGTTGCGATTA+TGG + chr1.3:34000667-34000686 MS.gene93276:intron 35.0%
!!! AAGCCTTTGGTTTTTGAATG+TGG + chr1.3:34001652-34001671 MS.gene93276:CDS 35.0%
!!! AGGGAAATGAGGTTGTTTTT+GGG - chr1.3:34000102-34000121 None:intergenic 35.0%
AAACAACCTCATTTCCCTCA+TGG + chr1.3:34000104-34000123 MS.gene93276:CDS 40.0%
AAATCAGAACCCTATCATGG+GGG + chr1.3:34000331-34000350 MS.gene93276:CDS 40.0%
ACTAGTGTGATTGTTTCAGG+TGG + chr1.3:34000995-34001014 MS.gene93276:CDS 40.0%
AGAAGGGGAAGTAATAGAAG+GGG - chr1.3:33999943-33999962 None:intergenic 40.0%
AGAGAACTCAGCATAGACTA+TGG + chr1.3:34002142-34002161 MS.gene93276:CDS 40.0%
AGGATAGTTGGATTGATACC+TGG + chr1.3:34000848-34000867 MS.gene93276:CDS 40.0%
AGGTGGTTATGAGGATGATA+TGG + chr1.3:34001012-34001031 MS.gene93276:CDS 40.0%
ATAGATAGATGGGCAGAATG+GGG + chr1.3:34001901-34001920 MS.gene93276:intron 40.0%
ATTCGTGGGATGAAGTATGA+AGG + chr1.3:34001178-34001197 MS.gene93276:CDS 40.0%
ATTGCATATAGCATGAGACG+GGG - chr1.3:34002103-34002122 None:intergenic 40.0%
CAAATCACTCCCATTTACAC+CGG + chr1.3:34000008-34000027 MS.gene93276:CDS 40.0%
CATCGCGATAAAAGGATAGT+TGG + chr1.3:34000836-34000855 MS.gene93276:CDS 40.0%
CATTGCATATAGCATGAGAC+GGG - chr1.3:34002104-34002123 None:intergenic 40.0%
CCATTGCATATAGCATGAGA+CGG - chr1.3:34002105-34002124 None:intergenic 40.0%
CCTAAAAGTAGGTCTTCCAA+TGG + chr1.3:34000959-34000978 MS.gene93276:CDS 40.0%
CTCTGATGAAACACAAACCA+CGG - chr1.3:34001520-34001539 None:intergenic 40.0%
GAGAAGGGGAAGTAATAGAA+GGG - chr1.3:33999944-33999963 None:intergenic 40.0%
GATGATATGGAATTGGACGA+TGG + chr1.3:34001025-34001044 MS.gene93276:CDS 40.0%
GATTGAGGGTTGACATGTTA+GGG - chr1.3:33999986-34000005 None:intergenic 40.0%
GCTACTAGTGTGATTGTTTC+AGG + chr1.3:34000992-34001011 MS.gene93276:CDS 40.0%
GGATTTATCGGAAGTAGCAT+TGG - chr1.3:34000225-34000244 None:intergenic 40.0%
GGTGAGAAGAAGTTTGAGAA+GGG - chr1.3:33999959-33999978 None:intergenic 40.0%
GGTGTAAATGGGAGTGATTT+GGG - chr1.3:34000009-34000028 None:intergenic 40.0%
GGTTTCAGATCTGAGTATGA+AGG + chr1.3:34000466-34000485 MS.gene93276:CDS 40.0%
GTAATAATCAATACCGGCCA+TGG + chr1.3:34001052-34001071 MS.gene93276:CDS 40.0%
GTGAGAAGAAGTTTGAGAAG+GGG - chr1.3:33999958-33999977 None:intergenic 40.0%
GTGGTGAAGAAATCCTCAAA+AGG - chr1.3:34000071-34000090 None:intergenic 40.0%
GTGTAAATGGGAGTGATTTG+GGG - chr1.3:34000008-34000027 None:intergenic 40.0%
GTTCTGATTTAGTCCAAGGT+CGG - chr1.3:34000321-34000340 None:intergenic 40.0%
TAGGTCCTTGGATCTTATTC+AGG + chr1.3:34001777-34001796 MS.gene93276:CDS 40.0%
TATGGTTGCATCGCGATAAA+AGG + chr1.3:34000828-34000847 MS.gene93276:CDS 40.0%
TCAACATTGCGAATTGTTGC+TGG + chr1.3:34002004-34002023 MS.gene93276:CDS 40.0%
TCAGTCAATATTTCCGACCT+TGG + chr1.3:34000305-34000324 MS.gene93276:CDS 40.0%
TCATATCCATGAGGGAAATG+AGG - chr1.3:34000113-34000132 None:intergenic 40.0%
TCTATGCTGAGTTCTCTCAA+TGG - chr1.3:34002139-34002158 None:intergenic 40.0%
TGAAGTTGCGATTATGGAGA+TGG + chr1.3:34000673-34000692 MS.gene93276:intron 40.0%
TGAGAAGGGGAAGTAATAGA+AGG - chr1.3:33999945-33999964 None:intergenic 40.0%
TGAGGAGAATATGTTGGATG+AGG + chr1.3:34000634-34000653 MS.gene93276:CDS 40.0%
TGGTGAGAAGAAGTTTGAGA+AGG - chr1.3:33999960-33999979 None:intergenic 40.0%
TGTGATCAGAAATTAGAGGG+TGG + chr1.3:34001106-34001125 MS.gene93276:CDS 40.0%
TGTTGTAGTACTCAGTGTAG+AGG - chr1.3:34000190-34000209 None:intergenic 40.0%
TTATGATTCGCTACGTGTGT+TGG + chr1.3:34000529-34000548 MS.gene93276:CDS 40.0%
TTCGTGGGATGAAGTATGAA+GGG + chr1.3:34001179-34001198 MS.gene93276:CDS 40.0%
TTGCATATGCAGATACAAGC+TGG + chr1.3:34000923-34000942 MS.gene93276:CDS 40.0%
TTGGAGCGTAGTATGCATTA+CGG + chr1.3:34001139-34001158 MS.gene93276:CDS 40.0%
! CCCAAAACCTAAACCTTTTG+AGG + chr1.3:34000055-34000074 MS.gene93276:CDS 40.0%
! GATTGTTTCAGGTGGTTATG+AGG + chr1.3:34001003-34001022 MS.gene93276:CDS 40.0%
! TAGGGTTCTGATTTAGTCCA+AGG - chr1.3:34000325-34000344 None:intergenic 40.0%
! TGGTTTGTGTTTCATCAGAG+CGG + chr1.3:34001520-34001539 MS.gene93276:CDS 40.0%
! TTATCGGAAGTAGCATTGGA+AGG - chr1.3:34000221-34000240 None:intergenic 40.0%
! TTCAGGGTTTGCTTGTGAAA+GGG + chr1.3:34001629-34001648 MS.gene93276:CDS 40.0%
!! ACGTGTGTTGGAAAGTATTG+AGG + chr1.3:34000541-34000560 MS.gene93276:CDS 40.0%
!! ACTGATTGCTGGTTTGAAGA+AGG + chr1.3:34001253-34001272 MS.gene93276:CDS 40.0%
!! CCATTGGAAGACCTACTTTT+AGG - chr1.3:34000962-34000981 None:intergenic 40.0%
!! CTGATTGCTGGTTTGAAGAA+GGG + chr1.3:34001254-34001273 MS.gene93276:CDS 40.0%
!! GAGGGAAATGAGGTTGTTTT+TGG - chr1.3:34000103-34000122 None:intergenic 40.0%
!! TTCCGAAAACTACTTTTCCG+TGG + chr1.3:34001500-34001519 MS.gene93276:CDS 40.0%
!!! AGGTTGTTTTTGGGAACCTT+GGG - chr1.3:34000093-34000112 None:intergenic 40.0%
!!! TTTAGGTTTTGGGACGAGTA+TGG - chr1.3:34000048-34000067 None:intergenic 40.0%
AAAGGGGATCAAGCATAGGT+TGG + chr1.3:34001720-34001739 MS.gene93276:CDS 45.0%
AACATGGAAGAGGTAGCAAC+AGG + chr1.3:34000716-34000735 MS.gene93276:intron 45.0%
AATCAACCTCATTTCCCTCC+TGG + chr1.3:34000140-34000159 MS.gene93276:CDS 45.0%
AATTGAGGGGCAAGCTAAGA+TGG + chr1.3:34001315-34001334 MS.gene93276:CDS 45.0%
ACAAAATCCACCCCCATGAT+AGG - chr1.3:34000344-34000363 None:intergenic 45.0%
ACATCGCCAACATAAACTCC+AGG - chr1.3:34000869-34000888 None:intergenic 45.0%
ACATGGAAGAGGTAGCAACA+GGG + chr1.3:34000717-34000736 MS.gene93276:intron 45.0%
AGAGGGAGAATGTTCCTGTT+AGG + chr1.3:34001428-34001447 MS.gene93276:CDS 45.0%
AGATGCATCGCAACATGATG+AGG + chr1.3:34000492-34000511 MS.gene93276:CDS 45.0%
ATAATCAATACCGGCCATGG+AGG + chr1.3:34001055-34001074 MS.gene93276:CDS 45.0%
ATCGAGATTAGGGTCATTCG+TGG + chr1.3:34001163-34001182 MS.gene93276:CDS 45.0%
ATCTTGTTCGACGGTAGCTA+TGG + chr1.3:34000412-34000431 MS.gene93276:CDS 45.0%
ATGCTGAGTTCTCTCAATGG+AGG - chr1.3:34002136-34002155 None:intergenic 45.0%
ATTGAGGGGCAAGCTAAGAT+GGG + chr1.3:34001316-34001335 MS.gene93276:CDS 45.0%
CAAAATCCACCCCCATGATA+GGG - chr1.3:34000343-34000362 None:intergenic 45.0%
CACAAAAGGGGATCAAGCAT+AGG + chr1.3:34001716-34001735 MS.gene93276:CDS 45.0%
CCGTCTCATGCTATATGCAA+TGG + chr1.3:34002102-34002121 MS.gene93276:CDS 45.0%
CCTTTATCTGCGAGTATACC+GGG + chr1.3:34001806-34001825 MS.gene93276:CDS 45.0%
CGCGATGCAACCATAACTTT+CGG - chr1.3:34000823-34000842 None:intergenic 45.0%
CGGAATCGTGTTGCACAAAA+GGG + chr1.3:34001703-34001722 MS.gene93276:CDS 45.0%
CGGTGTAAATGGGAGTGATT+TGG - chr1.3:34000010-34000029 None:intergenic 45.0%
CTTTATCTGCGAGTATACCG+GGG + chr1.3:34001807-34001826 MS.gene93276:CDS 45.0%
GACTGAGTTCTCCATTGAAG+CGG - chr1.3:34000291-34000310 None:intergenic 45.0%
GCAACACGATTCCGACAATT+GGG - chr1.3:34001697-34001716 None:intergenic 45.0%
GCAACATGATGAGGAGAACA+AGG + chr1.3:34000501-34000520 MS.gene93276:CDS 45.0%
GCATTACGGGATCGAGATTA+GGG + chr1.3:34001153-34001172 MS.gene93276:CDS 45.0%
GCCTTTATCTGCGAGTATAC+CGG + chr1.3:34001805-34001824 MS.gene93276:CDS 45.0%
GCGATTATGGAGATGGAGAT+GGG + chr1.3:34000680-34000699 MS.gene93276:intron 45.0%
GGAATCGTGTTGCACAAAAG+GGG + chr1.3:34001704-34001723 MS.gene93276:CDS 45.0%
GGATTGAGGGTTGACATGTT+AGG - chr1.3:33999987-34000006 None:intergenic 45.0%
GGGCCACATTCAAAAACCAA+AGG - chr1.3:34001658-34001677 None:intergenic 45.0%
GGGTTGACATGTTAGGGATT+TGG - chr1.3:33999980-33999999 None:intergenic 45.0%
GGTTTGTGTTTCATCAGAGC+GGG + chr1.3:34001521-34001540 MS.gene93276:CDS 45.0%
TAGATAGATGGGCAGAATGG+GGG + chr1.3:34001902-34001921 MS.gene93276:intron 45.0%
TCATATCCAGGAGGGAAATG+AGG - chr1.3:34000149-34000168 None:intergenic 45.0%
TCCAGCCTGAATAAGATCCA+AGG - chr1.3:34001785-34001804 None:intergenic 45.0%
TCCTTGGATCTTATTCAGGC+TGG + chr1.3:34001781-34001800 MS.gene93276:CDS 45.0%
TCGAGATTAGGGTCATTCGT+GGG + chr1.3:34001164-34001183 MS.gene93276:CDS 45.0%
TCGGAATCGTGTTGCACAAA+AGG + chr1.3:34001702-34001721 MS.gene93276:CDS 45.0%
TCGTGGGATGAAGTATGAAG+GGG + chr1.3:34001180-34001199 MS.gene93276:CDS 45.0%
TGCAACACGATTCCGACAAT+TGG - chr1.3:34001698-34001717 None:intergenic 45.0%
TGCATTACGGGATCGAGATT+AGG + chr1.3:34001152-34001171 MS.gene93276:CDS 45.0%
TGCGATTATGGAGATGGAGA+TGG + chr1.3:34000679-34000698 MS.gene93276:intron 45.0%
TGCTGAGTTCTCTCAATGGA+GGG - chr1.3:34002135-34002154 None:intergenic 45.0%
TGGAGCGTAGTATGCATTAC+GGG + chr1.3:34001140-34001159 MS.gene93276:CDS 45.0%
TGGGAGGACAAGAGCAAAAA+GGG - chr1.3:34001678-34001697 None:intergenic 45.0%
TGGTTTGAAGAAGGGATGTC+TGG + chr1.3:34001262-34001281 MS.gene93276:CDS 45.0%
TTGCATATAGCATGAGACGG+GGG - chr1.3:34002102-34002121 None:intergenic 45.0%
TTGGGAGGACAAGAGCAAAA+AGG - chr1.3:34001679-34001698 None:intergenic 45.0%
! AGCTACTGGTTGTGAATGTG+TGG + chr1.3:34001546-34001565 MS.gene93276:CDS 45.0%
! CTTCAGGGTTTGCTTGTGAA+AGG + chr1.3:34001628-34001647 MS.gene93276:CDS 45.0%
! GAAGTAGCATTGGAAGGTTG+TGG - chr1.3:34000215-34000234 None:intergenic 45.0%
! GGCTGATGAAGTTTTTGTGG+AGG + chr1.3:34000595-34000614 MS.gene93276:CDS 45.0%
! TACTGGTTGTGAATGTGTGG+AGG + chr1.3:34001549-34001568 MS.gene93276:CDS 45.0%
! TGGTGATGAGAACATGGAAG+AGG + chr1.3:34000706-34000725 MS.gene93276:intron 45.0%
!! AGCATAGGTTGGAAGTGTTC+AGG + chr1.3:34001731-34001750 MS.gene93276:CDS 45.0%
!! CAATGCTTTTGCCGCTTCAA+TGG + chr1.3:34000277-34000296 MS.gene93276:CDS 45.0%
!! TATCATGGGGGTGGATTTTG+TGG + chr1.3:34000343-34000362 MS.gene93276:CDS 45.0%
!! TGAAGCGGCAAAAGCATTGT+TGG - chr1.3:34000276-34000295 None:intergenic 45.0%
!!! GAGGTTGTTTTTGGGAACCT+TGG - chr1.3:34000094-34000113 None:intergenic 45.0%
!!! TTGTTTTTGGGAACCTTGGG+TGG - chr1.3:34000090-34000109 None:intergenic 45.0%
AAGGGCTGCGAGGTTAATGA+AGG + chr1.3:34000796-34000815 MS.gene93276:CDS 50.0%
ACACGATTCCGACAATTGGG+AGG - chr1.3:34001694-34001713 None:intergenic 50.0%
AGAGGGTGGAAACCTTGCTT+TGG + chr1.3:34001120-34001139 MS.gene93276:CDS 50.0%
AGATAGATGGGCAGAATGGG+GGG + chr1.3:34001903-34001922 MS.gene93276:intron 50.0%
AGGCAAGTCAGATGCTTGTG+AGG + chr1.3:34000444-34000463 MS.gene93276:CDS 50.0%
CATGGAAGAGGTAGCAACAG+GGG + chr1.3:34000718-34000737 MS.gene93276:intron 50.0%
CCCGGTATACTCGCAGATAA+AGG - chr1.3:34001809-34001828 None:intergenic 50.0%
CGTGGGATGAAGTATGAAGG+GGG + chr1.3:34001181-34001200 MS.gene93276:CDS 50.0%
CTTCTTTCTTCCTCCATGGC+CGG - chr1.3:34001068-34001087 None:intergenic 50.0%
GAGGATTTCTTCACCACCCA+AGG + chr1.3:34000074-34000093 MS.gene93276:CDS 50.0%
GCATACTACGCTCCAAAGCA+AGG - chr1.3:34001135-34001154 None:intergenic 50.0%
GCTCTTGTCCTCCCAATTGT+CGG + chr1.3:34001683-34001702 MS.gene93276:CDS 50.0%
GCTTGTGAAAGGGAAGCCTT+TGG + chr1.3:34001639-34001658 MS.gene93276:CDS 50.0%
GGCAAGTCAGATGCTTGTGA+GGG + chr1.3:34000445-34000464 MS.gene93276:CDS 50.0%
GGGACGAGTATGGTTTGAAG+CGG - chr1.3:34000038-34000057 None:intergenic 50.0%
GTAGTTTCATAAGGGCTGCG+AGG + chr1.3:34000786-34000805 MS.gene93276:CDS 50.0%
GTGGAGGGGGAAATGTTGAA+TGG + chr1.3:34000740-34000759 MS.gene93276:CDS 50.0%
TGTGGAGGCAGTGAAGAGAT+TGG + chr1.3:34000610-34000629 MS.gene93276:CDS 50.0%
TGTGTGGAGGCTTGTACTGA+TGG + chr1.3:34001562-34001581 MS.gene93276:CDS 50.0%
! GGCAGTGAAGAGATTGGTTG+AGG + chr1.3:34000616-34000635 MS.gene93276:CDS 50.0%
! GGGAGTGATTTGGGGATTGA+GGG - chr1.3:34000000-34000019 None:intergenic 50.0%
! TATGGTTTGAAGCGGTGCTC+CGG - chr1.3:34000030-34000049 None:intergenic 50.0%
! TGGGAGTGATTTGGGGATTG+AGG - chr1.3:34000001-34000020 None:intergenic 50.0%
!! AGTAGCAATTGGCTCGCCAT+TGG - chr1.3:34000978-34000997 None:intergenic 50.0%
AAGCGGTGCTCCGGTGTAAA+TGG - chr1.3:34000021-34000040 None:intergenic 55.0%
ACTATGGCATCCCTGACGAG+TGG + chr1.3:34002158-34002177 MS.gene93276:CDS 55.0%
AGCGGTGCTCCGGTGTAAAT+GGG - chr1.3:34000020-34000039 None:intergenic 55.0%
AGGTCTGCGCAAACTGGTTG+GGG + chr1.3:34001751-34001770 MS.gene93276:CDS 55.0%
ATGGAGATGGAGATGGGAGC+TGG + chr1.3:34000686-34000705 MS.gene93276:intron 55.0%
CAGGTCTGCGCAAACTGGTT+GGG + chr1.3:34001750-34001769 MS.gene93276:CDS 55.0%
CATCAGAGCGGGAAAGCTAC+TGG + chr1.3:34001532-34001551 MS.gene93276:CDS 55.0%
CGCAAACTGGTTGGGGAGTT+AGG + chr1.3:34001758-34001777 MS.gene93276:CDS 55.0%
CTATGGCATCCCTGACGAGT+GGG + chr1.3:34002159-34002178 MS.gene93276:CDS 55.0%
GATAGATGGGCAGAATGGGG+GGG + chr1.3:34001904-34001923 MS.gene93276:intron 55.0%
GTGTTCAGGTCTGCGCAAAC+TGG + chr1.3:34001745-34001764 MS.gene93276:CDS 55.0%
TCAGAACCCTATCATGGGGG+TGG + chr1.3:34000334-34000353 MS.gene93276:CDS 55.0%
TCAGGTCTGCGCAAACTGGT+TGG + chr1.3:34001749-34001768 MS.gene93276:CDS 55.0%
TGGTTGGGGAGTTAGGTCCT+TGG + chr1.3:34001765-34001784 MS.gene93276:CDS 55.0%
! GGGAGCTGGTGATGAGAACA+TGG + chr1.3:34000700-34000719 MS.gene93276:intron 55.0%
AGAGGTAGCAACAGGGGTGG+AGG + chr1.3:34000724-34000743 MS.gene93276:intron 60.0%
AGGTAGCAACAGGGGTGGAG+GGG + chr1.3:34000726-34000745 MS.gene93276:intron 60.0%
GAGGTAGCAACAGGGGTGGA+GGG + chr1.3:34000725-34000744 MS.gene93276:intron 60.0%
GGAAGAGGTAGCAACAGGGG+TGG + chr1.3:34000721-34000740 MS.gene93276:intron 60.0%
!! GGGTGCGTCTAGCTCTGGTA+AGG + chr1.3:34001201-34001220 MS.gene93276:CDS 60.0%
GGTAGCAACAGGGGTGGAGG+GGG + chr1.3:34000727-34000746 MS.gene93276:intron 65.0%
TGGCATCCCTGACGAGTGGG+CGG + chr1.3:34002162-34002181 MS.gene93276:CDS 65.0%
! GAAGGGGGTGCGTCTAGCTC+TGG + chr1.3:34001196-34001215 MS.gene93276:CDS 65.0%
AGCTTGCCCGCCCACTCGTC+AGG - chr1.3:34002172-34002191 None:intergenic 70.0%
GCTTGCCCGCCCACTCGTCA+GGG - chr1.3:34002171-34002190 None:intergenic 70.0%
GGCATCCCTGACGAGTGGGC+GGG + chr1.3:34002163-34002182 MS.gene93276:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 33999903 34002207 33999903 ID=MS.gene93276
chr1.3 mRNA 33999903 34002207 33999903 ID=MS.gene93276.t1;Parent=MS.gene93276
chr1.3 exon 33999903 34000676 33999903 ID=MS.gene93276.t1.exon1;Parent=MS.gene93276.t1
chr1.3 CDS 33999903 34000676 33999903 ID=cds.MS.gene93276.t1;Parent=MS.gene93276.t1
chr1.3 exon 34000728 34001828 34000728 ID=MS.gene93276.t1.exon2;Parent=MS.gene93276.t1
chr1.3 CDS 34000728 34001828 34000728 ID=cds.MS.gene93276.t1;Parent=MS.gene93276.t1
chr1.3 exon 34001905 34002207 34001905 ID=MS.gene93276.t1.exon3;Parent=MS.gene93276.t1
chr1.3 CDS 34001905 34002207 34001905 ID=cds.MS.gene93276.t1;Parent=MS.gene93276.t1
Gene Sequence

>MS.gene93276

ATGAATCCAATAGATCATTTATCATTAACCCTTGAAACCCCTTCTATTACTTCCCCTTCTCAAACTTCTTCTCACCAAATCCCTAACATGTCAACCCTCAATCCCCAAATCACTCCCATTTACACCGGAGCACCGCTTCAAACCATACTCGTCCCAAAACCTAAACCTTTTGAGGATTTCTTCACCACCCAAGGTTCCCAAAAACAACCTCATTTCCCTCATGGATATGAACACAACAATCAACCTCATTTCCCTCCTGGATATGAACACAAAAATCAATCAAACCTCTACACTGAGTACTACAACATTCCACAACCTTCCAATGCTACTTCCGATAAATCCCTTTATCATTTTCTCAAAATTGATGATTCCAACAATGCTTTTGCCGCTTCAATGGAGAACTCAGTCAATATTTCCGACCTTGGACTAAATCAGAACCCTATCATGGGGGTGGATTTTGTGGATGATAGAATTGATCAATCTATTGTGCCCATTTCAAAGAAACAAGAATCTTGTTCGACGGTAGCTATGGTTAAGAAAAAGGCAAGTCAGATGCTTGTGAGGGTTTCAGATCTGAGTATGAAGGATCAGATGCATCGCAACATGATGAGGAGAACAAGGATGACTTATGATTCGCTACGTGTGTTGGAAAGTATTGAGGATGAGAAAAGAATTATTGAGGAGAAGAAATTGGCTGATGAAGTTTTTGTGGAGGCAGTGAAGAGATTGGTTGAGGAGAATATGTTGGATGAGGATAGGATATTTGTTTCTGAAGTTGCGATTATGGAGATGGAGATGGGAGCTGGTGATGAGAACATGGAAGAGGTAGCAACAGGGGTGGAGGGGGAAATGTTGAATGGAAGAAGACATCATAATCATAGTCGTAGTTTCATAAGGGCTGCGAGGTTAATGAAGGACCGAAAGTTATGGTTGCATCGCGATAAAAGGATAGTTGGATTGATACCTGGAGTTTATGTTGGCGATGTTTTCTTGTATATGGTGGAATTATGTGTAATTGGTTTGCATATGCAGATACAAGCTGGCATTGATTTTCTTCCTAAAAGTAGGTCTTCCAATGGCGAGCCAATTGCTACTAGTGTGATTGTTTCAGGTGGTTATGAGGATGATATGGAATTGGACGATGGTGATGTAATAATCAATACCGGCCATGGAGGAAGAAAGAAGAATTCCTCAATTCAAATTTGTGATCAGAAATTAGAGGGTGGAAACCTTGCTTTGGAGCGTAGTATGCATTACGGGATCGAGATTAGGGTCATTCGTGGGATGAAGTATGAAGGGGGTGCGTCTAGCTCTGGTAAGGTTTATGTTTATGATGGATTATATAGGATTACTGATTGCTGGTTTGAAGAAGGGATGTCTGGTTTTAGAGTTTTTAAGTATAAGTTATTGAGAATTGAGGGGCAAGCTAAGATGGGTAGTGTTGTTCTAAAAAAGGCTAGGGAGATTAAGAAAATTGGGTTGGATGTTAAGCCTATGTATTGTCTTTCTGTTGATATTTCACACAAGAGGGAGAATGTTCCTGTTAGGCTATTTAATGACATAGATGACAATCAAGAACCTCTTTATTATGAGTATCTTCCGAAAACTACTTTTCCGTGGTTTGTGTTTCATCAGAGCGGGAAAGCTACTGGTTGTGAATGTGTGGAGGCTTGTACTGATGGCTGCTTTTGCTCTATGAAGAATGGAGGAGAGTTTCCCTATAATCTTCAGGGTTTGCTTGTGAAAGGGAAGCCTTTGGTTTTTGAATGTGGCCCTTTTTGCTCTTGTCCTCCCAATTGTCGGAATCGTGTTGCACAAAAGGGGATCAAGCATAGGTTGGAAGTGTTCAGGTCTGCGCAAACTGGTTGGGGAGTTAGGTCCTTGGATCTTATTCAGGCTGGAGCCTTTATCTGCGAGTATACCGGGGTAATTTTAACAAGGGAACAAGCTCAAATTTTAACAATGAATGGTGACTCGTTAATATATCCAAAACGGTTTATAGATAGATGGGCAGAATGGGGGGGATTTGTCTCAGATATACAATAATTATGTTCGTCCATCATATCAATTAATACCTGATATAGATTATTCTATGGATATATCAACATTGCGAATTGTTGCTGGCTATATGAGCCATAGTTCGAGTCCAAATGTATTTGTTCAATTTGTTCTCTATGATCATAACAATCTGATGTTCCCCCGTCTCATGCTATATGCAATGGAGAACATCCCTCCATTGAGAGAACTCAGCATAGACTATGGCATCCCTGACGAGTGGGCGGGCAAGCTATCAATATGTATGTAA
Protein sequence

>MS.gene93276.t1

MNPIDHLSLTLETPSITSPSQTSSHQIPNMSTLNPQITPIYTGAPLQTILVPKPKPFEDFFTTQGSQKQPHFPHGYEHNNQPHFPPGYEHKNQSNLYTEYYNIPQPSNATSDKSLYHFLKIDDSNNAFAASMENSVNISDLGLNQNPIMGVDFVDDRIDQSIVPISKKQESCSTVAMVKKKASQMLVRVSDLSMKDQMHRNMMRRTRMTYDSLRVLESIEDEKRIIEEKKLADEVFVEAVKRLVEENMLDEDRIFVSEVATGVEGEMLNGRRHHNHSRSFIRAARLMKDRKLWLHRDKRIVGLIPGVYVGDVFLYMVELCVIGLHMQIQAGIDFLPKSRSSNGEPIATSVIVSGGYEDDMELDDGDVIINTGHGGRKKNSSIQICDQKLEGGNLALERSMHYGIEIRVIRGMKYEGGASSSGKVYVYDGLYRITDCWFEEGMSGFRVFKYKLLRIEGQAKMGSVVLKKAREIKKIGLDVKPMYCLSVDISHKRENVPVRLFNDIDDNQEPLYYEYLPKTTFPWFVFHQSGKATGCECVEACTDGCFCSMKNGGEFPYNLQGLLVKGKPLVFECGPFCSCPPNCRNRVAQKGIKHRLEVFRSAQTGWGVRSLDLIQAGAFICEYTGIDGQNGGDLSQIYNNYVRPSYQLIPDIDYSMDISTLRIVAGYMSHSSSPNVFVQFVLYDHNNLMFPRLMLYAMENIPPLRELSIDYGIPDEWAGKLSICM