Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93317.t1 | AES63738.2 | 32.6 | 89 | 60 | 0 | 29 | 117 | 17 | 105 | 1.30E-07 | 66.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93317.t1 | G7INX6 | 32.6 | 89 | 60 | 0 | 29 | 117 | 17 | 105 | 9.4e-08 | 66.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene93317.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93317.t1 | MTR_2g012700 | 32.584 | 89 | 60 | 0 | 29 | 117 | 17 | 105 | 1.23e-13 | 63.2 |
| MS.gene93317.t1 | MTR_2g062330 | 30.337 | 89 | 61 | 1 | 29 | 117 | 37 | 124 | 6.23e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTACTTACCCGAAAATTTC+TGG | 0.121099 | 1.3:+33585079 | None:intergenic |
| TCTTTATCATTGGGAAATTA+AGG | 0.246824 | 1.3:-33585123 | MS.gene93317:CDS |
| ATCTCCAGCATCTTCCTAAA+AGG | 0.249787 | 1.3:-33584946 | MS.gene93317:intron |
| AGCAAGTTGTTCTTTATCAT+TGG | 0.320120 | 1.3:-33585133 | MS.gene93317:CDS |
| TATGTTTGCCCCATTAAAAC+TGG | 0.383833 | 1.3:-33584854 | MS.gene93317:CDS |
| TTGCGCAGAACAGCGTTCAT+TGG | 0.396003 | 1.3:-33585178 | MS.gene93317:CDS |
| GCAAGTTGTTCTTTATCATT+GGG | 0.404910 | 1.3:-33585132 | MS.gene93317:CDS |
| GAGGATAAGTATATCTATAA+AGG | 0.409031 | 1.3:-33584818 | MS.gene93317:CDS |
| TCAATCACACAGGGTGTTGC+CGG | 0.416330 | 1.3:+33584699 | None:intergenic |
| TGTTCGTGACAACGAGTTGA+AGG | 0.462873 | 1.3:-33584783 | MS.gene93317:CDS |
| ACGAGTTGAAGGTCAATGAT+AGG | 0.471795 | 1.3:-33584772 | MS.gene93317:CDS |
| AGGATAATCACACGTTGATC+CGG | 0.481188 | 1.3:+33584902 | None:intergenic |
| TCGTGGAGGTTAAAATTCTC+CGG | 0.489434 | 1.3:-33584718 | MS.gene93317:CDS |
| TCGTAAAGGTTTCGACGACA+TGG | 0.514522 | 1.3:-33585206 | MS.gene93317:intron |
| ATACTTATCCTCAACACCTC+TGG | 0.529619 | 1.3:+33584829 | None:intergenic |
| AAAGGTTTCGACGACATGGA+AGG | 0.531990 | 1.3:-33585202 | MS.gene93317:intron |
| TGTGAGGGAGGTGATGCATC+CGG | 0.535867 | 1.3:-33584921 | MS.gene93317:CDS |
| AAGGTTTCGACGACATGGAA+GGG | 0.536796 | 1.3:-33585201 | MS.gene93317:CDS |
| AACTGGTACCAGAGGTGTTG+AGG | 0.541872 | 1.3:-33584837 | MS.gene93317:CDS |
| GGTCACAGAGAGCGTTGCTA+TGG | 0.546123 | 1.3:-33585102 | MS.gene93317:CDS |
| ATAAGTATATCTATAAAGGC+TGG | 0.555742 | 1.3:-33584814 | MS.gene93317:CDS |
| CATATTGAAATGCATGTATG+AGG | 0.560024 | 1.3:-33584747 | MS.gene93317:CDS |
| ATCTTCCTAAAAGGTATGTG+AGG | 0.561486 | 1.3:-33584937 | MS.gene93317:CDS |
| CCATGGCAATTTCTTCGAGA+AGG | 0.564688 | 1.3:-33585357 | MS.gene93317:intron |
| CATGTATGAGGTTGAATTCG+TGG | 0.565338 | 1.3:-33584735 | MS.gene93317:CDS |
| TAAACATACTTCAATCACAC+AGG | 0.592278 | 1.3:+33584689 | None:intergenic |
| GTGATTATCCTCTATGATGT+TGG | 0.597229 | 1.3:-33584890 | MS.gene93317:CDS |
| TCCTAAAAGGTATGTGAGGG+AGG | 0.598055 | 1.3:-33584933 | MS.gene93317:CDS |
| CCCATTAAAACTGGTACCAG+AGG | 0.609643 | 1.3:-33584845 | MS.gene93317:CDS |
| TCTTCCTAAAAGGTATGTGA+GGG | 0.638638 | 1.3:-33584936 | MS.gene93317:CDS |
| CCTTCTCGAAGAAATTGCCA+TGG | 0.641633 | 1.3:+33585357 | None:intergenic |
| TGTCTTGACCAACATCATAG+AGG | 0.651392 | 1.3:+33584882 | None:intergenic |
| TTGGATTGACGAAGATACTG+AGG | 0.654652 | 1.3:-33585159 | MS.gene93317:CDS |
| AAACATACTTCAATCACACA+GGG | 0.675982 | 1.3:+33584690 | None:intergenic |
| GTATGAGGTTGAATTCGTGG+AGG | 0.713812 | 1.3:-33584732 | MS.gene93317:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAATATTCAATAATCAGAT+AGG | - | chr1.3:33584795-33584814 | MS.gene93317:CDS | 20.0% |
| ! | ATAAGTATATCTATAAAGGC+TGG | - | chr1.3:33585244-33585263 | MS.gene93317:intron | 25.0% |
| ! | GAGGATAAGTATATCTATAA+AGG | - | chr1.3:33585240-33585259 | MS.gene93317:intron | 25.0% |
| ! | TCTTTATCATTGGGAAATTA+AGG | - | chr1.3:33584935-33584954 | MS.gene93317:CDS | 25.0% |
| AGCAAGTTGTTCTTTATCAT+TGG | - | chr1.3:33584925-33584944 | MS.gene93317:CDS | 30.0% | |
| ATTACTTACCCGAAAATTTC+TGG | + | chr1.3:33584982-33585001 | None:intergenic | 30.0% | |
| CATATTGAAATGCATGTATG+AGG | - | chr1.3:33585311-33585330 | MS.gene93317:intron | 30.0% | |
| GCAAGTTGTTCTTTATCATT+GGG | - | chr1.3:33584926-33584945 | MS.gene93317:CDS | 30.0% | |
| TTTACGACACATTTAGACAA+AGG | + | chr1.3:33584839-33584858 | None:intergenic | 30.0% | |
| TTTGTCTAAATGTGTCGTAA+AGG | - | chr1.3:33584838-33584857 | MS.gene93317:CDS | 30.0% | |
| !! | TTATCTGGCTTATTCTGTTT+TGG | - | chr1.3:33584734-33584753 | MS.gene93317:CDS | 30.0% |
| !!! | GAAGGTATTCTGTTTTTATC+TGG | - | chr1.3:33584719-33584738 | MS.gene93317:CDS | 30.0% |
| GTGATTATCCTCTATGATGT+TGG | - | chr1.3:33585168-33585187 | MS.gene93317:CDS | 35.0% | |
| TATGTTTGCCCCATTAAAAC+TGG | - | chr1.3:33585204-33585223 | MS.gene93317:intron | 35.0% | |
| ! | ATCTTCCTAAAAGGTATGTG+AGG | - | chr1.3:33585121-33585140 | MS.gene93317:CDS | 35.0% |
| ! | TCTTCCTAAAAGGTATGTGA+GGG | - | chr1.3:33585122-33585141 | MS.gene93317:CDS | 35.0% |
| ! | TTGCTATGGCCAGAAATTTT+CGG | - | chr1.3:33584970-33584989 | MS.gene93317:intron | 35.0% |
| !!! | TGGCTTATTCTGTTTTGGTA+TGG | - | chr1.3:33584739-33584758 | MS.gene93317:CDS | 35.0% |
| AGGATAATCACACGTTGATC+CGG | + | chr1.3:33585159-33585178 | None:intergenic | 40.0% | |
| ATACTTATCCTCAACACCTC+TGG | + | chr1.3:33585232-33585251 | None:intergenic | 40.0% | |
| ATCTCCAGCATCTTCCTAAA+AGG | - | chr1.3:33585112-33585131 | MS.gene93317:CDS | 40.0% | |
| CATGTATGAGGTTGAATTCG+TGG | - | chr1.3:33585323-33585342 | MS.gene93317:intron | 40.0% | |
| GAATGATATCGCTTTGACTG+AGG | - | chr1.3:33585029-33585048 | MS.gene93317:intron | 40.0% | |
| TCGTGGAGGTTAAAATTCTC+CGG | - | chr1.3:33585340-33585359 | MS.gene93317:intron | 40.0% | |
| TGTCTTGACCAACATCATAG+AGG | + | chr1.3:33585179-33585198 | None:intergenic | 40.0% | |
| TTGGATTGACGAAGATACTG+AGG | - | chr1.3:33584899-33584918 | MS.gene93317:CDS | 40.0% | |
| ! | ACGAGTTGAAGGTCAATGAT+AGG | - | chr1.3:33585286-33585305 | MS.gene93317:intron | 40.0% |
| ! | TGCTATGGCCAGAAATTTTC+GGG | - | chr1.3:33584971-33584990 | MS.gene93317:intron | 40.0% |
| !! | ACCTCTGGTACCAGTTTTAA+TGG | + | chr1.3:33585217-33585236 | None:intergenic | 40.0% |
| !! | CATACCTTTTAGGAAGATGC+TGG | + | chr1.3:33585119-33585138 | None:intergenic | 40.0% |
| !! | CCTCTGGTACCAGTTTTAAT+GGG | + | chr1.3:33585216-33585235 | None:intergenic | 40.0% |
| !! | CTCTGGTACCAGTTTTAATG+GGG | + | chr1.3:33585215-33585234 | None:intergenic | 40.0% |
| AAAGGTTTCGACGACATGGA+AGG | - | chr1.3:33584856-33584875 | MS.gene93317:CDS | 45.0% | |
| AAGGTTTCGACGACATGGAA+GGG | - | chr1.3:33584857-33584876 | MS.gene93317:CDS | 45.0% | |
| CCCATTAAAACTGGTACCAG+AGG | - | chr1.3:33585213-33585232 | MS.gene93317:intron | 45.0% | |
| GTATGAGGTTGAATTCGTGG+AGG | - | chr1.3:33585326-33585345 | MS.gene93317:intron | 45.0% | |
| TCGTAAAGGTTTCGACGACA+TGG | - | chr1.3:33584852-33584871 | MS.gene93317:CDS | 45.0% | |
| ! | ACCTCCCTCACATACCTTTT+AGG | + | chr1.3:33585129-33585148 | None:intergenic | 45.0% |
| ! | CGCTTTGACTGAGGTTCTTT+TGG | - | chr1.3:33585038-33585057 | MS.gene93317:intron | 45.0% |
| ! | TCCTAAAAGGTATGTGAGGG+AGG | - | chr1.3:33585125-33585144 | MS.gene93317:CDS | 45.0% |
| ! | TGTTCGTGACAACGAGTTGA+AGG | - | chr1.3:33585275-33585294 | MS.gene93317:intron | 45.0% |
| !! | ATAAAAAAAACTTAAATTTA+AGG | + | chr1.3:33585094-33585113 | None:intergenic | 5.0% |
| !! | TAAAAAAAACTTAAATTTAA+GGG | + | chr1.3:33585093-33585112 | None:intergenic | 5.0% |
| TTGCGCAGAACAGCGTTCAT+TGG | - | chr1.3:33584880-33584899 | MS.gene93317:CDS | 50.0% | |
| !! | AACTGGTACCAGAGGTGTTG+AGG | - | chr1.3:33585221-33585240 | MS.gene93317:intron | 50.0% |
| TGTGAGGGAGGTGATGCATC+CGG | - | chr1.3:33585137-33585156 | MS.gene93317:CDS | 55.0% | |
| !! | GGTCACAGAGAGCGTTGCTA+TGG | - | chr1.3:33584956-33584975 | MS.gene93317:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 33584703 | 33585377 | 33584703 | ID=MS.gene93317 |
| chr1.3 | mRNA | 33584703 | 33585377 | 33584703 | ID=MS.gene93317.t1;Parent=MS.gene93317 |
| chr1.3 | exon | 33585358 | 33585377 | 33585358 | ID=MS.gene93317.t1.exon1;Parent=MS.gene93317.t1 |
| chr1.3 | CDS | 33585358 | 33585377 | 33585358 | ID=cds.MS.gene93317.t1;Parent=MS.gene93317.t1 |
| chr1.3 | exon | 33585088 | 33585220 | 33585088 | ID=MS.gene93317.t1.exon2;Parent=MS.gene93317.t1 |
| chr1.3 | CDS | 33585088 | 33585220 | 33585088 | ID=cds.MS.gene93317.t1;Parent=MS.gene93317.t1 |
| chr1.3 | exon | 33584703 | 33584960 | 33584703 | ID=MS.gene93317.t1.exon3;Parent=MS.gene93317.t1 |
| chr1.3 | CDS | 33584703 | 33584960 | 33584703 | ID=cds.MS.gene93317.t1;Parent=MS.gene93317.t1 |
| Gene Sequence |
| Protein sequence |
