AlfalfaGEDB | MS.gene93754

M. sativa cultivar XinJiangDaYe / MS.gene93754

KEGG: -
GO: -
PFAM: PF06200.17 PF09425.13

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93754.t1	XP_003631116.2	83.7	129	6	3	1	125	1	118	7.00E-48	199.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93754.t1	Q8LBM2	42.0	131	64	6	1	124	3	128	1.9e-14	80.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93754.t1	G7LE60	83.7	129	6	3	1	125	1	118	5.0e-48	199.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene93754.t1	TF	Tify

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93754.t1	MTR_8g107300	83.721	129	6	3	1	125	1	118	2.49e-70	206
MS.gene93754.t1	MTR_2g019190	76.471	136	19	3	1	125	1	134	9.65e-68	200
MS.gene93754.t1	MTR_8g107300	82.979	94	5	1	32	125	1	83	1.01e-48	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93754.t1	AT1G30135	41.912	136	57	7	1	124	3	128	2.13e-21	83.2
MS.gene93754.t1	AT2G34600	39.062	128	72	5	1	124	20	145	1.25e-16	71.2

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Find 26 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAATGAAGAGATCTTTGCAA+CGG	0.550495	4.2:+232965	MS.gene93754:CDS
CAATTGTATAGCCCTGGGTC+TGG	0.482257	4.2:+232937	MS.gene93754:CDS
CAGAATCATAGAGAGGAAAA+AGG	0.376693	4.2:-232460	None:intergenic
CATCTTCCCATCATAGAATA+TGG	0.389620	4.2:-232671	None:intergenic
CCAGGGCTATACAATTGATG+AGG	0.668057	4.2:-232931	None:intergenic
CCATCATAGAATATGGTCAG+CGG	0.671722	4.2:-232664	None:intergenic
CCGCTGACCATATTCTATGA+TGG	0.457336	4.2:+232664	MS.gene93754:CDS
CCTCATCAATTGTATAGCCC+TGG	0.405940	4.2:+232931	MS.gene93754:CDS
CGCTGACCATATTCTATGAT+GGG	0.509614	4.2:+232665	MS.gene93754:CDS
CTCATCAATTGTATAGCCCT+GGG	0.470700	4.2:+232932	MS.gene93754:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTACAGAAGAGAAAGAATA+GGG	+	chr4.2:232990-233009	MS.gene93754:CDS	30.0%
TTCTACAGAAGAGAAAGAAT+AGG	+	chr4.2:232989-233008	MS.gene93754:CDS	30.0%
TTTATTTGCCAGCATTAAGA+TGG	-	chr4.2:232842-232861	None:intergenic	30.0%
CAATGAAGAGATCTTTGCAA+CGG	+	chr4.2:232965-232984	MS.gene93754:CDS	35.0%
CAGAATCATAGAGAGGAAAA+AGG	-	chr4.2:232463-232482	None:intergenic	35.0%
CATCTTCCCATCATAGAATA+TGG	-	chr4.2:232674-232693	None:intergenic	35.0%
GCAAAGATCTCTTCATTGAA+AGG	-	chr4.2:232963-232982	None:intergenic	35.0%
GCATTCTCTTATCTTACATG+TGG	-	chr4.2:232508-232527	None:intergenic	35.0%
TTCTCTTATCTTACATGTGG+TGG	-	chr4.2:232505-232524	None:intergenic	35.0%
AAAAAGTGAAGACACCGAGA+GGG	+	chr4.2:232875-232894	MS.gene93754:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	232431	233041	232431	ID=MS.gene93754
chr4.2	mRNA	232431	233041	232431	ID=MS.gene93754.t1;Parent=MS.gene93754
chr4.2	exon	232431	232510	232431	ID=MS.gene93754.t1.exon1;Parent=MS.gene93754.t1
chr4.2	CDS	232431	232510	232431	ID=cds.MS.gene93754.t1;Parent=MS.gene93754.t1
chr4.2	exon	232633	232720	232633	ID=MS.gene93754.t1.exon2;Parent=MS.gene93754.t1
chr4.2	CDS	232633	232720	232633	ID=cds.MS.gene93754.t1;Parent=MS.gene93754.t1
chr4.2	exon	232832	233041	232832	ID=MS.gene93754.t1.exon3;Parent=MS.gene93754.t1
chr4.2	CDS	232832	233041	232832	ID=cds.MS.gene93754.t1;Parent=MS.gene93754.t1

Gene Sequence

>MS.gene93754

ATGAGGAGGAATTGTAACTTAGAACTCTGCCTTTTTCCTCTCTATGATTCTGGCAATCACAACAACAGCAACCACCACATGTAAGATAAGAGAATGCTTTTCTTTGTATACCATTTCCATTTTTTCAATTTGTTAGACTATGATTTTCTTTTTATATTTGGGTATTAAAGTAATTCTTTGTTAATTTCTATCTCCATTGAAGGGAGGAAAGCCCAATGCAAAATCAGGAACAGCCGCTGACCATATTCTATGATGGGAAGATGTGTCTGACTGATGTCACAGAGTTTCAGGTATACTCATTACCGCCCTCTGTTCTTGACTAGGCCAATAACTAAATGAAAAAATGATTTTGCAATCTCTTTATAAAAATAAATAAATGGGTTAATGTGAATGTTGTGCAGGCCAAATCCATCTTAATGCTGGCAAATAAAATTAAGTTGCAAGAAAAAGTGAAGACACCGAGAGGGTCAGAGCGAACAACACCAACAATAGTGCAGCCTCCTCATCAATTGTATAGCCCTGGGTCTGGCCTTTCAATGAAGAGATCTTTGCAACGGTTTCTACAGAAGAGAAAGAATAGGGTCCAAGAAGCATCTCCATACCATCACTAG

Protein sequence

>MS.gene93754.t1

MRRNCNLELCLFPLYDSGNHNNSNHHMEESPMQNQEQPLTIFYDGKMCLTDVTEFQAKSILMLANKIKLQEKVKTPRGSERTTPTIVQPPHQLYSPGSGLSMKRSLQRFLQKRKNRVQEASPYHH