Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93934.t1 | GAU37750.1 | 38.4 | 151 | 92 | 1 | 2 | 151 | 59 | 209 | 7.90E-22 | 113.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93934.t1 | A0A2Z6NMK1 | 38.4 | 151 | 92 | 1 | 2 | 151 | 59 | 209 | 5.7e-22 | 113.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene93934.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCAACTTAAGGGAGTTTA+TGG | 0.164205 | 4.3:-66499215 | MS.gene93934:CDS |
| AATGATCTTTATAAAGTTCT+TGG | 0.244817 | 4.3:-66499177 | MS.gene93934:CDS |
| CAGATGTAATGAAGGTTTCT+AGG | 0.299323 | 4.3:-66498710 | MS.gene93934:CDS |
| TGATAACCTGCAGCCATATA+AGG | 0.324757 | 4.3:+66499035 | None:intergenic |
| AATGACCTTAACTCCTTATA+TGG | 0.332349 | 4.3:-66499048 | MS.gene93934:CDS |
| ATGATCTTTATAAAGTTCTT+GGG | 0.339041 | 4.3:-66499176 | MS.gene93934:CDS |
| CAGTACATCACATTTGTACT+TGG | 0.354754 | 4.3:+66498851 | None:intergenic |
| CTGAGGGATGGATCAACTTA+AGG | 0.380199 | 4.3:-66499225 | None:intergenic |
| GTCCACGAAATGCAGTGAAT+TGG | 0.385092 | 4.3:+66499141 | None:intergenic |
| TACTTTGCTATTAATATGTA+TGG | 0.399529 | 4.3:-66498885 | MS.gene93934:CDS |
| TAAGCTTAAGTTTAAGTTTG+TGG | 0.399998 | 4.3:-66498745 | MS.gene93934:CDS |
| AACTTAAGGGAGTTTATGGT+TGG | 0.422168 | 4.3:-66499211 | MS.gene93934:CDS |
| GGTGATTACATTAGAAGCTC+AGG | 0.426576 | 4.3:-66498909 | MS.gene93934:CDS |
| CAGACACTGAACATTGACTT+TGG | 0.433491 | 4.3:-66498930 | MS.gene93934:CDS |
| TTCCAATTCACTGCATTTCG+TGG | 0.438395 | 4.3:-66499143 | MS.gene93934:CDS |
| ACAAATGTGATGTACTGATA+AGG | 0.441312 | 4.3:-66498845 | MS.gene93934:CDS |
| TGCAGCCATATAAGGAGTTA+AGG | 0.444382 | 4.3:+66499043 | None:intergenic |
| TATGGCTGCAGGTTATCAAT+TGG | 0.445570 | 4.3:-66499030 | MS.gene93934:intron |
| GAGTTAAGGTCATTTCATAA+TGG | 0.446526 | 4.3:+66499057 | None:intergenic |
| ATTAATATGTATGGACCTAC+AGG | 0.484413 | 4.3:-66498876 | MS.gene93934:CDS |
| TGAGGGATGGATCAACTTAA+GGG | 0.485967 | 4.3:-66499224 | None:intergenic |
| TTAACTCCTTATATGGCTGC+AGG | 0.501456 | 4.3:-66499041 | MS.gene93934:CDS |
| TGTAAAGACAATGGATTCCA+TGG | 0.503947 | 4.3:-66498774 | MS.gene93934:CDS |
| CAAATGTGATGTACTGATAA+GGG | 0.512356 | 4.3:-66498844 | MS.gene93934:CDS |
| CACTTACAAGCAAGGAATTG+AGG | 0.514420 | 4.3:-66499087 | MS.gene93934:CDS |
| TGCTTGTAAGTGTGCGATGA+TGG | 0.527718 | 4.3:+66499098 | None:intergenic |
| AAGGCAACAAAGATTGGTAG+TGG | 0.537973 | 4.3:-66498810 | MS.gene93934:CDS |
| TGATCTTTATAAAGTTCTTG+GGG | 0.551565 | 4.3:-66499175 | MS.gene93934:CDS |
| AAGACAATGGATTCCATGGT+GGG | 0.554852 | 4.3:-66498770 | MS.gene93934:CDS |
| AAGTACATCAGATGTAATGA+AGG | 0.558515 | 4.3:-66498718 | MS.gene93934:CDS |
| CATTTGTACTTGGTGCCTGT+AGG | 0.577136 | 4.3:+66498861 | None:intergenic |
| ACTTAAGCTTATCCCCACCA+TGG | 0.632346 | 4.3:+66498757 | None:intergenic |
| CAACAAAGATTGGTAGTGGG+TGG | 0.633130 | 4.3:-66498806 | MS.gene93934:CDS |
| AAAGACAATGGATTCCATGG+TGG | 0.635851 | 4.3:-66498771 | MS.gene93934:CDS |
| AGACAATGGATTCCATGGTG+GGG | 0.640570 | 4.3:-66498769 | MS.gene93934:CDS |
| TCATCGCACACTTACAAGCA+AGG | 0.641070 | 4.3:-66499095 | MS.gene93934:CDS |
| TGTGATGTACTGATAAGGGA+TGG | 0.644855 | 4.3:-66498840 | MS.gene93934:CDS |
| AGGCAACAAAGATTGGTAGT+GGG | 0.660836 | 4.3:-66498809 | MS.gene93934:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGATCTTTATAAAGTTCT+TGG | - | chr4.3:66498745-66498764 | MS.gene93934:CDS | 20.0% |
| !! | ATGATCTTTATAAAGTTCTT+GGG | - | chr4.3:66498746-66498765 | MS.gene93934:CDS | 20.0% |
| !! | TAGAGATATATCATTTGATA+AGG | + | chr4.3:66498933-66498952 | None:intergenic | 20.0% |
| !!! | AACAAATTTTGTAAAGACAA+TGG | - | chr4.3:66499139-66499158 | MS.gene93934:CDS | 20.0% |
| !!! | TACTTTGCTATTAATATGTA+TGG | - | chr4.3:66499037-66499056 | MS.gene93934:CDS | 20.0% |
| ! | TGATCTTTATAAAGTTCTTG+GGG | - | chr4.3:66498747-66498766 | MS.gene93934:CDS | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | - | chr4.3:66499177-66499196 | MS.gene93934:CDS | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | - | chr4.3:66499106-66499125 | MS.gene93934:CDS | 30.0% | |
| AAGTACATCAGATGTAATGA+AGG | - | chr4.3:66499204-66499223 | MS.gene93934:CDS | 30.0% | |
| AATGACCTTAACTCCTTATA+TGG | - | chr4.3:66498874-66498893 | MS.gene93934:CDS | 30.0% | |
| ATCATTTGATAAGGAGTTGT+TGG | + | chr4.3:66498924-66498943 | None:intergenic | 30.0% | |
| ATTAATATGTATGGACCTAC+AGG | - | chr4.3:66499046-66499065 | MS.gene93934:CDS | 30.0% | |
| GAGTTAAGGTCATTTCATAA+TGG | + | chr4.3:66498868-66498887 | None:intergenic | 30.0% | |
| ! | ACAAATGTGATGTACTGATA+AGG | - | chr4.3:66499077-66499096 | MS.gene93934:CDS | 30.0% |
| ! | CAAATGTGATGTACTGATAA+GGG | - | chr4.3:66499078-66499097 | MS.gene93934:CDS | 30.0% |
| AACTTAAGGGAGTTTATGGT+TGG | - | chr4.3:66498711-66498730 | MS.gene93934:CDS | 35.0% | |
| CAGATGTAATGAAGGTTTCT+AGG | - | chr4.3:66499212-66499231 | MS.gene93934:CDS | 35.0% | |
| CAGTACATCACATTTGTACT+TGG | + | chr4.3:66499074-66499093 | None:intergenic | 35.0% | |
| GATAAGGGATGGAAAAAAGA+AGG | - | chr4.3:66499093-66499112 | MS.gene93934:CDS | 35.0% | |
| GATCAACTTAAGGGAGTTTA+TGG | - | chr4.3:66498707-66498726 | MS.gene93934:CDS | 35.0% | |
| ! | TGTAAAGACAATGGATTCCA+TGG | - | chr4.3:66499148-66499167 | MS.gene93934:CDS | 35.0% |
| AAGGCAACAAAGATTGGTAG+TGG | - | chr4.3:66499112-66499131 | MS.gene93934:CDS | 40.0% | |
| CACTTACAAGCAAGGAATTG+AGG | - | chr4.3:66498835-66498854 | MS.gene93934:CDS | 40.0% | |
| GGTGATTACATTAGAAGCTC+AGG | - | chr4.3:66499013-66499032 | MS.gene93934:intron | 40.0% | |
| TATGGCTGCAGGTTATCAAT+TGG | - | chr4.3:66498892-66498911 | MS.gene93934:CDS | 40.0% | |
| TGATAACCTGCAGCCATATA+AGG | + | chr4.3:66498890-66498909 | None:intergenic | 40.0% | |
| TGCAGCCATATAAGGAGTTA+AGG | + | chr4.3:66498882-66498901 | None:intergenic | 40.0% | |
| TTAACTCCTTATATGGCTGC+AGG | - | chr4.3:66498881-66498900 | MS.gene93934:CDS | 40.0% | |
| TTCCAATTCACTGCATTTCG+TGG | - | chr4.3:66498779-66498798 | MS.gene93934:CDS | 40.0% | |
| ! | AAAGACAATGGATTCCATGG+TGG | - | chr4.3:66499151-66499170 | MS.gene93934:CDS | 40.0% |
| ! | AAGACAATGGATTCCATGGT+GGG | - | chr4.3:66499152-66499171 | MS.gene93934:CDS | 40.0% |
| ! | AGGCAACAAAGATTGGTAGT+GGG | - | chr4.3:66499113-66499132 | MS.gene93934:CDS | 40.0% |
| ! | TGTGATGTACTGATAAGGGA+TGG | - | chr4.3:66499082-66499101 | MS.gene93934:CDS | 40.0% |
| !! | CAGACACTGAACATTGACTT+TGG | - | chr4.3:66498992-66499011 | MS.gene93934:intron | 40.0% |
| ACTTAAGCTTATCCCCACCA+TGG | + | chr4.3:66499168-66499187 | None:intergenic | 45.0% | |
| GTCCACGAAATGCAGTGAAT+TGG | + | chr4.3:66498784-66498803 | None:intergenic | 45.0% | |
| TCATCGCACACTTACAAGCA+AGG | - | chr4.3:66498827-66498846 | MS.gene93934:CDS | 45.0% | |
| TGCTTGTAAGTGTGCGATGA+TGG | + | chr4.3:66498827-66498846 | None:intergenic | 45.0% | |
| ! | AGACAATGGATTCCATGGTG+GGG | - | chr4.3:66499153-66499172 | MS.gene93934:CDS | 45.0% |
| !! | CAACAAAGATTGGTAGTGGG+TGG | - | chr4.3:66499116-66499135 | MS.gene93934:CDS | 45.0% |
| !! | CATTTGTACTTGGTGCCTGT+AGG | + | chr4.3:66499064-66499083 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 66498704 | 66499240 | 66498704 | ID=MS.gene93934 |
| chr4.3 | mRNA | 66498704 | 66499240 | 66498704 | ID=MS.gene93934.t1;Parent=MS.gene93934 |
| chr4.3 | exon | 66499031 | 66499240 | 66499031 | ID=MS.gene93934.t1.exon1;Parent=MS.gene93934.t1 |
| chr4.3 | CDS | 66499031 | 66499240 | 66499031 | ID=cds.MS.gene93934.t1;Parent=MS.gene93934.t1 |
| chr4.3 | exon | 66498704 | 66498949 | 66498704 | ID=MS.gene93934.t1.exon2;Parent=MS.gene93934.t1 |
| chr4.3 | CDS | 66498704 | 66498949 | 66498704 | ID=cds.MS.gene93934.t1;Parent=MS.gene93934.t1 |
| Gene Sequence |
| Protein sequence |
