AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94080.t1 MTR_5g058860 96.647 507 13 3 5 508 1 506 0.0 1005
MS.gene94080.t1 MTR_3g072710 61.014 513 161 12 16 502 4 503 0.0 572
MS.gene94080.t1 MTR_8g093070 37.438 406 216 14 103 499 120 496 2.61e-63 214
MS.gene94080.t1 MTR_7g027190 35.749 414 218 14 108 500 288 674 6.33e-63 217
MS.gene94080.t1 MTR_7g074650 35.027 374 218 10 126 498 442 791 1.31e-52 191
MS.gene94080.t1 MTR_3g065980 34.314 408 209 15 116 498 166 539 1.40e-51 184
MS.gene94080.t1 MTR_1g069725 33.499 403 234 11 101 500 93 464 7.37e-49 175
MS.gene94080.t1 MTR_4g076020 35.262 363 192 12 151 506 110 436 3.88e-47 169
MS.gene94080.t1 MTR_8g442410 29.207 517 308 18 13 498 35 524 4.87e-44 163
MS.gene94080.t1 MTR_5g097480 30.591 389 242 11 119 498 174 543 1.33e-43 162
MS.gene94080.t1 MTR_3g056110 32.329 365 221 11 143 498 194 541 5.22e-42 157
MS.gene94080.t1 MTR_1g096030 30.918 414 240 12 112 501 70 461 2.05e-41 154
MS.gene94080.t1 MTR_4g104020 29.808 416 260 10 98 500 125 521 3.43e-41 155
MS.gene94080.t1 MTR_1g029420 31.525 387 237 14 121 498 224 591 3.99e-41 156
MS.gene94080.t1 MTR_2g082090 31.186 388 241 11 119 498 209 578 9.20e-41 155
MS.gene94080.t1 MTR_4g077760 31.897 348 221 7 153 498 221 554 7.55e-40 152
MS.gene94080.t1 MTR_3g089055 30.052 386 244 11 121 498 197 564 1.50e-39 151
MS.gene94080.t1 MTR_3g089055 30.052 386 244 11 121 498 202 569 1.64e-39 151
MS.gene94080.t1 MTR_4g102790 31.188 404 233 17 115 499 285 662 5.73e-38 148
MS.gene94080.t1 MTR_4g133660 29.692 357 226 10 153 500 204 544 1.79e-37 145
MS.gene94080.t1 MTR_5g094450 27.706 462 291 14 54 498 96 531 1.22e-34 137
MS.gene94080.t1 MTR_2g097410 27.021 433 269 15 90 499 333 741 2.54e-33 134
MS.gene94080.t1 MTR_2g026250 26.611 357 237 10 153 500 257 597 3.48e-33 133
MS.gene94080.t1 MTR_4g064200 28.133 391 244 15 122 499 284 650 2.71e-30 125
MS.gene94080.t1 MTR_4g064150 27.924 419 259 15 100 499 339 733 4.14e-30 125
MS.gene94080.t1 MTR_2g097467 27.095 358 232 9 152 499 316 654 6.43e-30 124
MS.gene94080.t1 MTR_0092s0100 29.557 406 257 14 99 500 352 732 9.05e-29 120
MS.gene94080.t1 MTR_0092s0100 29.557 406 257 14 99 500 349 729 1.00e-28 120
MS.gene94080.t1 MTR_4g064120 25.056 443 282 13 80 501 212 625 1.25e-28 120
MS.gene94080.t1 MTR_2g034250 25.926 405 264 11 111 498 199 584 4.54e-28 118
MS.gene94080.t1 MTR_7g062120 24.450 409 280 10 100 498 350 739 2.48e-27 116
MS.gene94080.t1 MTR_8g077940 29.067 375 234 13 134 500 192 542 3.56e-27 115
MS.gene94080.t1 MTR_2g034260 26.277 411 260 15 106 498 198 583 4.71e-27 115
MS.gene94080.t1 MTR_2g097463 26.816 358 233 10 152 499 317 655 4.77e-27 115
MS.gene94080.t1 MTR_4g064180 26.056 426 259 16 100 501 232 625 6.13e-27 115
MS.gene94080.t1 MTR_3g022830 24.331 411 222 10 151 498 51 435 7.29e-27 113
MS.gene94080.t1 MTR_4g064160 26.179 424 277 12 87 499 286 684 7.36e-27 115
MS.gene94080.t1 MTR_2g097473 26.536 358 234 9 152 499 316 654 8.76e-27 114
MS.gene94080.t1 MTR_2g099110 29.552 335 200 12 93 411 37 351 1.17e-25 109
MS.gene94080.t1 MTR_2g097390 26.873 387 252 13 122 498 275 640 4.41e-25 109
MS.gene94080.t1 MTR_4g097080 26.425 386 261 11 121 498 132 502 1.88e-24 107
MS.gene94080.t1 MTR_2g034280 24.505 404 274 12 106 498 191 574 4.22e-24 106
MS.gene94080.t1 MTR_4g076140 32.051 234 135 6 108 337 34 247 5.76e-24 105
MS.gene94080.t1 MTR_5g015490 27.681 401 243 15 121 498 113 489 6.47e-24 105
MS.gene94080.t1 MTR_2g097310 24.806 387 262 11 122 499 272 638 2.33e-23 104
MS.gene94080.t1 MTR_5g009080 31.780 236 137 7 109 340 43 258 3.55e-23 102
MS.gene94080.t1 MTR_2g097350 26.098 387 255 12 122 498 274 639 6.19e-23 102
MS.gene94080.t1 MTR_7g109580 25.182 413 265 16 103 501 174 556 2.17e-22 100
MS.gene94080.t1 MTR_2g097380 24.930 357 213 10 152 499 251 561 3.93e-22 100
MS.gene94080.t1 MTR_4g026485 25.714 385 250 14 123 500 270 625 1.36e-19 92.4
MS.gene94080.t1 MTR_7g069740 26.634 413 252 18 105 498 203 583 1.45e-18 89.4
MS.gene94080.t1 MTR_7g057230 27.742 310 195 10 200 498 365 656 2.46e-15 79.3
MS.gene94080.t1 MTR_1g086970 22.983 409 274 13 111 501 95 480 2.66e-15 78.6
MS.gene94080.t1 MTR_2g089100 24.634 410 271 12 116 499 46 443 1.04e-14 76.6
MS.gene94080.t1 MTR_6g047750 26.087 391 249 15 123 498 296 661 9.30e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94080.t1 AT4G08250 51.020 490 206 13 23 498 7 476 1.29e-147 432
MS.gene94080.t1 AT3G54220 36.070 402 223 13 108 502 279 653 1.97e-54 194
MS.gene94080.t1 AT3G03450 31.027 477 272 14 57 498 90 544 2.54e-53 189
MS.gene94080.t1 AT5G41920 35.661 401 220 14 107 498 28 399 1.60e-51 181
MS.gene94080.t1 AT5G17490 35.338 399 223 15 113 504 151 521 1.53e-50 181
MS.gene94080.t1 AT1G66350 32.737 391 221 13 116 498 149 505 3.89e-46 169
MS.gene94080.t1 AT2G01570 32.997 397 228 16 111 498 213 580 1.65e-44 165
MS.gene94080.t1 AT1G14920 31.959 388 234 13 116 498 166 528 2.10e-43 161
MS.gene94080.t1 AT5G48150 30.809 383 242 10 123 498 123 489 1.87e-42 158
MS.gene94080.t1 AT5G48150 30.809 383 242 10 123 498 123 489 1.87e-42 158
MS.gene94080.t1 AT5G48150 30.809 383 242 10 123 498 123 489 1.87e-42 158
MS.gene94080.t1 AT5G48150 30.809 383 242 10 123 498 123 489 1.87e-42 158
MS.gene94080.t1 AT2G04890 30.612 392 247 11 110 498 43 412 2.21e-40 150
MS.gene94080.t1 AT1G55580 32.208 385 206 12 155 498 74 444 3.21e-40 151
MS.gene94080.t1 AT4G17230 32.242 397 229 12 117 498 153 524 3.32e-40 152
MS.gene94080.t1 AT4G17230 32.242 397 229 12 117 498 153 524 3.32e-40 152
MS.gene94080.t1 AT1G50600 28.632 468 299 13 39 498 85 525 3.10e-39 150
MS.gene94080.t1 AT3G50650 32.787 366 216 10 144 498 195 541 6.94e-39 149
MS.gene94080.t1 AT1G50600 31.233 365 225 10 143 498 253 600 8.43e-39 149
MS.gene94080.t1 AT1G50600 31.233 365 225 10 143 498 249 596 8.52e-39 149
MS.gene94080.t1 AT5G66770 30.971 381 245 9 122 498 217 583 1.06e-38 149
MS.gene94080.t1 AT1G63100 29.512 410 245 13 112 499 242 629 4.94e-34 136
MS.gene94080.t1 AT1G63100 29.512 410 245 13 112 499 267 654 6.85e-34 136
MS.gene94080.t1 AT1G21450 26.923 494 304 17 18 498 143 592 4.08e-32 130
MS.gene94080.t1 AT4G37650 28.030 396 253 11 122 498 146 528 6.54e-30 123
MS.gene94080.t1 AT4G36710 29.380 371 227 13 138 498 139 484 1.63e-29 121
MS.gene94080.t1 AT2G29065 27.569 399 246 11 122 499 252 628 3.60e-29 122
MS.gene94080.t1 AT3G60630 28.606 409 260 11 107 500 232 623 5.76e-29 121
MS.gene94080.t1 AT3G46600 27.027 407 229 14 121 498 3 370 9.29e-28 114
MS.gene94080.t1 AT3G46600 25.450 444 263 14 84 498 174 578 9.51e-28 117
MS.gene94080.t1 AT1G07530 26.173 405 275 9 103 499 377 765 1.30e-27 117
MS.gene94080.t1 AT3G46600 25.450 444 263 14 84 498 142 546 2.24e-27 115
MS.gene94080.t1 AT2G29060 25.951 447 281 16 71 501 245 657 3.60e-27 115
MS.gene94080.t1 AT2G29060 25.951 447 281 16 71 501 282 694 4.84e-27 115
MS.gene94080.t1 AT5G59450 25.688 436 276 13 87 498 186 597 1.67e-26 114
MS.gene94080.t1 AT1G07520 25.806 434 291 9 82 499 275 693 1.33e-24 108
MS.gene94080.t1 AT1G07520 25.806 434 291 9 82 499 276 694 1.39e-24 108
MS.gene94080.t1 AT2G45160 27.295 403 242 16 119 500 268 640 1.80e-24 107
MS.gene94080.t1 AT1G07520 25.909 440 283 10 82 499 111 529 3.15e-24 106
MS.gene94080.t1 AT1G07520 25.909 440 283 10 82 499 111 529 3.15e-24 106
MS.gene94080.t1 AT2G37650 25.707 389 260 11 119 498 332 700 1.36e-22 102
MS.gene94080.t1 AT2G37650 25.707 389 260 11 119 498 344 712 1.58e-22 102
MS.gene94080.t1 AT4G00150 28.125 384 237 13 123 502 209 557 3.23e-22 100
MS.gene94080.t1 AT1G50420 31.579 228 136 6 113 337 48 258 3.61e-22 99.8
MS.gene94080.t1 AT1G50420 30.921 152 102 3 350 500 331 480 2.64e-12 69.3
MS.gene94080.t1 AT5G52510 27.864 323 200 10 196 498 330 639 7.74e-17 84.0
MS.gene94080.t1 AT3G49950 25.250 400 262 14 121 501 29 410 3.53e-14 74.7
MS.gene94080.t1 AT3G13840 23.750 480 304 13 58 498 53 509 1.43e-12 70.5

Find 169 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGGTTTCCTATGAATAAA+TGG 0.181822 5.4:-46185580 MS.gene94080:CDS
GACTCTTGTTTGTGCCTGTT+AGG 0.230972 5.4:+46186386 None:intergenic
TTGTCAAGCAAAGTTGTTAT+TGG 0.254379 5.4:-46185396 MS.gene94080:CDS
GCAACCTTGTTATCTAGATT+TGG 0.271570 5.4:+46186541 None:intergenic
CGGTTCCGTTTAGCAGAGTT+TGG 0.294952 5.4:+46186263 None:intergenic
CTTCACCAGTCTTGTACAAC+TGG 0.307827 5.4:+46185501 None:intergenic
TTGGAGAATTCTAGAAAATC+TGG 0.308659 5.4:+46186709 None:intergenic
TTTGCCACTGTTCAAGAAAC+TGG 0.314348 5.4:-46185929 MS.gene94080:CDS
GGAAACAATAAGTTGGTGTT+AGG 0.333033 5.4:-46185338 MS.gene94080:CDS
GGGGAGTGGTTGGGTGTGGA+AGG 0.333319 5.4:-46185452 MS.gene94080:CDS
CCGTCTAGTGCACCTCCTTA+TGG 0.339880 5.4:-46186422 MS.gene94080:CDS
TGTCAAGCAAAGTTGTTATT+GGG 0.347692 5.4:-46185395 MS.gene94080:CDS
AGGCTTTGTGGGTTTGTTCA+TGG 0.365262 5.4:-46185645 MS.gene94080:CDS
ATTTGCCACATCTTAGTTAC+CGG 0.366851 5.4:-46185766 MS.gene94080:CDS
GGTTGCTTTCTGCTTCTGTT+TGG 0.368542 5.4:-46185301 MS.gene94080:CDS
CTCCTAGCCCATTTATTCAT+AGG 0.371100 5.4:+46185572 None:intergenic
GCGGCAGCGGAGGCCCTAAC+AGG 0.386266 5.4:-46186400 MS.gene94080:CDS
CATAGTGGACTTTGATATCA+TGG 0.386901 5.4:-46186080 MS.gene94080:CDS
GCTTGTTCGTGGAGAGGCTT+TGG 0.387556 5.4:-46185807 MS.gene94080:CDS
ACAATAAGTTGGTGTTAGGT+TGG 0.396110 5.4:-46185334 MS.gene94080:CDS
CATCAACGATGCAGGCTTTG+TGG 0.396922 5.4:-46185657 MS.gene94080:CDS
GGAGCAAGAGAGGTTCTCTT+GGG 0.397324 5.4:-46185474 MS.gene94080:CDS
TTTCATGAGTAACAGCTTCA+AGG 0.400010 5.4:+46186113 None:intergenic
ATGTATGACTCGTTCGAGGC+TGG 0.402016 5.4:-46185599 MS.gene94080:CDS
TTGGTCCCCTCTCCTTGACT+TGG 0.402311 5.4:-46186641 MS.gene94080:CDS
AGGAGCAAGAGAGGTTCTCT+TGG 0.407648 5.4:-46185475 MS.gene94080:CDS
CGTCAAGTTTGCTCATTTCA+CGG 0.410498 5.4:-46186149 MS.gene94080:CDS
ATCAACGATGCAGGCTTTGT+GGG 0.413507 5.4:-46185656 MS.gene94080:CDS
ACTCTTGTTTGTGCCTGTTA+GGG 0.415722 5.4:+46186387 None:intergenic
TCTATTGCTTCCTTCTTGAA+TGG 0.416138 5.4:-46185734 MS.gene94080:CDS
GGGAGTGGTTGGGTGTGGAA+GGG 0.418452 5.4:-46185451 MS.gene94080:CDS
CTCCGTTGTGGAATTTGAGT+TGG 0.418723 5.4:+46186474 None:intergenic
CACAAAGCCTGCATCGTTGA+TGG 0.420533 5.4:+46185658 None:intergenic
TGTATGACTCGTTCGAGGCT+GGG 0.428573 5.4:-46185598 MS.gene94080:CDS
GGGCCTCCGCTGCCGCCATA+AGG 0.429881 5.4:+46186407 None:intergenic
TGCGAAGATGTGGGCCCGGC+AGG 0.434472 5.4:+46186005 None:intergenic
TTATCTAGATTTGGAGCAAC+GGG 0.447526 5.4:+46186550 None:intergenic
CCTCCTTATGGCGGCAGCGG+AGG 0.448204 5.4:-46186410 MS.gene94080:CDS
GCCACTGAGCCCATTATTCG+TGG 0.457871 5.4:+46185524 None:intergenic
TGGGTATAGAGTGGAAGAGT+TGG 0.459148 5.4:-46185360 MS.gene94080:CDS
GGTGGTAGTGTTACTGTGAT+TGG 0.459740 5.4:+46186690 None:intergenic
TCGCGAAAAGAAGGCCTGCC+GGG 0.460380 5.4:-46186019 MS.gene94080:CDS
GGAGGAGGAGGATGACAGAA+AGG 0.460604 5.4:-46186449 MS.gene94080:CDS
GGGTATAGAGTGGAAGAGTT+GGG 0.462213 5.4:-46185359 MS.gene94080:CDS
AAGCTGTTACTCATGAAAGA+AGG 0.464529 5.4:-46186108 MS.gene94080:CDS
GAGCTTTGTTCCTAGCTCCT+TGG 0.469001 5.4:+46185709 None:intergenic
TAACAGCTTCAAGGATAGCT+TGG 0.472914 5.4:+46186122 None:intergenic
AACGGGTGTGATTTCGTCGT+TGG 0.474325 5.4:+46186567 None:intergenic
TCTTCATTAAAGCTTGTTCG+TGG 0.479110 5.4:-46185818 MS.gene94080:CDS
TTCCTTCTTGAATGGTGCCA+AGG 0.479649 5.4:-46185726 MS.gene94080:CDS
ACATTAGTCGAGGAGGAAGT+AGG 0.480496 5.4:-46185683 MS.gene94080:CDS
TATCATGGAAGGGGCCCAAT+GGG 0.484349 5.4:-46186065 MS.gene94080:CDS
GCCCTCCAAACTCTGCTAAA+CGG 0.486218 5.4:-46186268 MS.gene94080:CDS
TTCCACAACGGAGGAGGAGG+AGG 0.486277 5.4:-46186464 MS.gene94080:CDS
TGGCTCAAGTGATATTGATT+CGG 0.487459 5.4:-46186357 MS.gene94080:CDS
GCACCTCCTTATGGCGGCAG+CGG 0.487716 5.4:-46186413 MS.gene94080:CDS
TGAGAGTATGTCTGTTTGGT+GGG 0.489093 5.4:+46186204 None:intergenic
ATCGCGAAAAGAAGGCCTGC+CGG 0.491290 5.4:-46186020 MS.gene94080:CDS
TGAAGTTGTGGAAGTCATCT+TGG 0.492730 5.4:+46186602 None:intergenic
ACAACCTTTGCTGCTTCCGT+TGG 0.496161 5.4:-46185896 MS.gene94080:CDS
TCACTTTCATCGCGAAAAGA+AGG 0.496428 5.4:-46186028 MS.gene94080:CDS
TTGAATGGTGCCAAGGAGCT+AGG 0.496980 5.4:-46185719 MS.gene94080:CDS
TATTGGGTTTATTTAACGAT+GGG 0.498402 5.4:-46185379 MS.gene94080:CDS
CAGTGAGAAGACAGAGTTTG+TGG 0.500911 5.4:+46186230 None:intergenic
CGTCATCATCAGAAGTGGGT+GGG 0.501316 5.4:+46186668 None:intergenic
CCTCCGCTGCCGCCATAAGG+AGG 0.504163 5.4:+46186410 None:intergenic
CACAACGGAGGAGGAGGAGG+AGG 0.507245 5.4:-46186461 MS.gene94080:CDS
AGGCTGAGAGTATGTCTGTT+TGG 0.507765 5.4:+46186200 None:intergenic
CAAGATGAAGAAGAAGAAGA+TGG 0.510277 5.4:-46186514 MS.gene94080:CDS
TGGGTTTCCTATGAATAAAT+GGG 0.510723 5.4:-46185579 MS.gene94080:CDS
AGAAGAAGAAGATGGATCCA+TGG 0.511049 5.4:-46186506 MS.gene94080:CDS
CTTCTGATGATGACGCCACT+TGG 0.513488 5.4:-46186660 MS.gene94080:CDS
TTGAATGAGTGAAGCCCATT+GGG 0.514305 5.4:+46186051 None:intergenic
AGTGATATTGATTCGGCTCA+AGG 0.514796 5.4:-46186350 MS.gene94080:CDS
CACAAACAAGAGTCACCATC+TGG 0.516410 5.4:-46186377 MS.gene94080:CDS
CTCAAATTCCACAACGGAGG+AGG 0.518603 5.4:-46186470 MS.gene94080:CDS
TCCGTTTAGCAGAGTTTGGA+GGG 0.520521 5.4:+46186267 None:intergenic
TTGGGTGTGGAAGGGTTCAA+AGG 0.525480 5.4:-46185443 MS.gene94080:CDS
TTCCGTTTAGCAGAGTTTGG+AGG 0.528085 5.4:+46186266 None:intergenic
ATAACAACTTTGCTTGACAA+TGG 0.528206 5.4:+46185399 None:intergenic
CACAAACTCTGTCTTCTCAC+TGG 0.528238 5.4:-46186229 MS.gene94080:CDS
ACAAAGCCTGCATCGTTGAT+GGG 0.528462 5.4:+46185659 None:intergenic
TGAAAGAAGGGTTCACATAG+TGG 0.529945 5.4:-46186095 MS.gene94080:CDS
ACGAACAAGCTTTAATGAAG+AGG 0.534324 5.4:+46185820 None:intergenic
GTCATCATCAGAAGTGGGTG+GGG 0.534927 5.4:+46186669 None:intergenic
GTAGGGCCCATCAACGATGC+AGG 0.535058 5.4:-46185665 MS.gene94080:CDS
TGAGTTGGACATGTCTCCCA+TGG 0.535777 5.4:+46186489 None:intergenic
TAAATGGGCTAGGAGTTTAG+TGG 0.535874 5.4:-46185564 MS.gene94080:CDS
AAAGGAGTTAACATAAGCTA+TGG 0.536826 5.4:-46185425 MS.gene94080:CDS
TCTTCCAGTTTCTTGAACAG+TGG 0.536983 5.4:+46185925 None:intergenic
TTATTGGGTTTATTTAACGA+TGG 0.539610 5.4:-46185380 MS.gene94080:CDS
CTGAGAGTATGTCTGTTTGG+TGG 0.539651 5.4:+46186203 None:intergenic
AGCGATGTATGACTCGTTCG+AGG 0.539838 5.4:-46185603 MS.gene94080:CDS
GAGGAGGAGGATGACAGAAA+GGG 0.544247 5.4:-46186448 MS.gene94080:CDS
GTGGAAGTCATCTTGGTTGG+TGG 0.544732 5.4:+46186609 None:intergenic
AGTTTCTTGAACAGTGGCAA+AGG 0.546696 5.4:+46185931 None:intergenic
TTCCTATGAATAAATGGGCT+AGG 0.550588 5.4:-46185574 MS.gene94080:CDS
GTGAAGAGGAGGAGCAAGAG+AGG 0.551124 5.4:-46185484 MS.gene94080:CDS
CTCCATGTTGGTGCCGTGAG+TGG 0.552588 5.4:+46186315 None:intergenic
CATTAGTCGAGGAGGAAGTA+GGG 0.559643 5.4:-46185682 MS.gene94080:CDS
GAAGAAGAAGATGGATCCAT+GGG 0.560634 5.4:-46186505 MS.gene94080:CDS
AGAGTTGGGAAACAATAAGT+TGG 0.563779 5.4:-46185345 MS.gene94080:CDS
GACTTAGTGTCCTCCACTCA+CGG 0.564053 5.4:-46186328 MS.gene94080:CDS
ATTTAACGATGGGTATAGAG+TGG 0.564807 5.4:-46185369 MS.gene94080:CDS
ATTAAAGCTTGTTCGTGGAG+AGG 0.565007 5.4:-46185813 MS.gene94080:CDS
TATTGACATAGACATGATCA+TGG 0.565364 5.4:-46186740 MS.gene94080:CDS
AAATTCCACAACGGAGGAGG+AGG 0.565824 5.4:-46186467 MS.gene94080:CDS
AAGAAATCCAAGTCAAGGAG+AGG 0.565976 5.4:+46186634 None:intergenic
TTGTCCAACGGAAGCAGCAA+AGG 0.565983 5.4:+46185892 None:intergenic
AGCTGTTACTCATGAAAGAA+GGG 0.566179 5.4:-46186107 MS.gene94080:CDS
TGGAGAATTCTAGAAAATCT+GGG 0.566834 5.4:+46186710 None:intergenic
GTGGCCCAGTTGTACAAGAC+TGG 0.567811 5.4:-46185506 MS.gene94080:CDS
GCAAGAGAGGTTCTCTTGGG+GGG 0.573233 5.4:-46185471 MS.gene94080:CDS
GTTGTGGAAGTCATCTTGGT+TGG 0.573299 5.4:+46186606 None:intergenic
GTGGACTTTGATATCATGGA+AGG 0.574432 5.4:-46186076 MS.gene94080:CDS
ATTGAATGAGTGAAGCCCAT+TGG 0.575421 5.4:+46186050 None:intergenic
CATGTCTTGAAGAAGCTGAA+AGG 0.576740 5.4:+46186180 None:intergenic
CTCCTTGGCACCATTCAAGA+AGG 0.577682 5.4:+46185724 None:intergenic
AAATGAGCAAACTTGACGTA+AGG 0.579384 5.4:+46186154 None:intergenic
CCACTGAGCCCATTATTCGT+GGG 0.580098 5.4:+46185525 None:intergenic
GGACTTTGATATCATGGAAG+GGG 0.580763 5.4:-46186074 MS.gene94080:CDS
ATATCATGGAAGGGGCCCAA+TGG 0.582076 5.4:-46186066 MS.gene94080:CDS
AGAAACAAAGAACGTGGCAA+TGG 0.585187 5.4:-46185968 MS.gene94080:CDS
CTCCACTCACGGCACCAACA+TGG 0.585629 5.4:-46186317 MS.gene94080:CDS
GTGATGCGAAGATGTGGGCC+CGG 0.587438 5.4:+46186001 None:intergenic
GTTATCTAGATTTGGAGCAA+CGG 0.590909 5.4:+46186549 None:intergenic
AAGCAATAGAATCTGAAGAC+CGG 0.592324 5.4:+46185747 None:intergenic
CTGTTCAAGAAACTGGAAGA+AGG 0.593746 5.4:-46185922 MS.gene94080:CDS
GTTGTACAAGACTGGTGAAG+AGG 0.596068 5.4:-46185498 MS.gene94080:CDS
CTGCTAAACGGAACCGACTG+TGG 0.597512 5.4:-46186256 MS.gene94080:CDS
GTACAAGACTGGTGAAGAGG+AGG 0.599329 5.4:-46185495 MS.gene94080:CDS
TCAATATCACTTGAGCCAGA+TGG 0.599918 5.4:+46186362 None:intergenic
ATGGGCTAGGAGTTTAGTGG+AGG 0.599983 5.4:-46185561 MS.gene94080:CDS
TGCTCCAAATCTAGATAACA+AGG 0.601003 5.4:-46186545 MS.gene94080:CDS
CCATAAGGAGGTGCACTAGA+CGG 0.601550 5.4:+46186422 None:intergenic
TAGCAGAGTTTGGAGGGCGT+CGG 0.602737 5.4:+46186273 None:intergenic
AGTGGCGTCATCATCAGAAG+TGG 0.602786 5.4:+46186663 None:intergenic
GAGTTTGTGGTGACCACAGT+CGG 0.604569 5.4:+46186243 None:intergenic
TGAGAGAATCAATGAAGTTG+TGG 0.606418 5.4:+46186590 None:intergenic
GAGCAAGAGAGGTTCTCTTG+GGG 0.606683 5.4:-46185473 MS.gene94080:CDS
AATGCAGTGATGCGAAGATG+TGG 0.609707 5.4:+46185995 None:intergenic
ATCATCAGAAGTGGGTGGGG+TGG 0.609794 5.4:+46186672 None:intergenic
CATGTTGGTGCCGTGAGTGG+AGG 0.611646 5.4:+46186318 None:intergenic
TGGACTTTGATATCATGGAA+GGG 0.613948 5.4:-46186075 MS.gene94080:CDS
GCGTCATCATCAGAAGTGGG+TGG 0.616450 5.4:+46186667 None:intergenic
CGCAGCGAGTCTCTCCATGT+TGG 0.618231 5.4:+46186303 None:intergenic
ATTGTATATGATCATGAACA+TGG 0.621187 5.4:-46186779 None:intergenic
AAAAGTGAACGGTTGTCCAA+CGG 0.621563 5.4:+46185880 None:intergenic
TGTTAGGTTGGAAATCAAGA+CGG 0.623526 5.4:-46185322 MS.gene94080:CDS
TGGTGAAGAAATCCAAGTCA+AGG 0.627017 5.4:+46186629 None:intergenic
GAACATGGAGATTAATGACA+TGG 0.634701 5.4:-46186764 MS.gene94080:CDS
TTCACCAGTCTTGTACAACT+GGG 0.634954 5.4:+46185502 None:intergenic
AGAAATCCAAGTCAAGGAGA+GGG 0.637230 5.4:+46186635 None:intergenic
CTACATTGATGAAAAGTGAA+CGG 0.641838 5.4:+46185869 None:intergenic
AGCAAGAGAGGTTCTCTTGG+GGG 0.646650 5.4:-46185472 MS.gene94080:CDS
GTGGCGTCATCATCAGAAGT+GGG 0.647661 5.4:+46186664 None:intergenic
GAAATCCAAGTCAAGGAGAG+GGG 0.653641 5.4:+46186636 None:intergenic
GAAGACCGGTAACTAAGATG+TGG 0.659795 5.4:+46185761 None:intergenic
GAGAGTATGTCTGTTTGGTG+GGG 0.663577 5.4:+46186205 None:intergenic
GTCAAGGAGAGGGGACCAAG+TGG 0.663632 5.4:+46186645 None:intergenic
GCTCGTGACATTAGTCGAGG+AGG 0.670792 5.4:-46185690 MS.gene94080:CDS
CCCACGAATAATGGGCTCAG+TGG 0.676277 5.4:-46185525 MS.gene94080:CDS
AAAGCTCGTGACATTAGTCG+AGG 0.678929 5.4:-46185693 MS.gene94080:CDS
ATGCAGTGATGCGAAGATGT+GGG 0.688648 5.4:+46185996 None:intergenic
TAAGGAGGTGCACTAGACGG+AGG 0.697542 5.4:+46186425 None:intergenic
CAACTCAAATTCCACAACGG+AGG 0.701611 5.4:-46186473 MS.gene94080:CDS
TCTAGTGCACCTCCTTATGG+CGG 0.711985 5.4:-46186419 MS.gene94080:CDS
GTCCAACTCAAATTCCACAA+CGG 0.724597 5.4:-46186476 MS.gene94080:CDS
TTGTCTAGAAACAAAGAACG+TGG 0.726054 5.4:-46185974 MS.gene94080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATTGGGTTTATTTAACGAT+GGG - chr5.4:46186661-46186680 MS.gene94080:CDS 25.0%
! TTATTGGGTTTATTTAACGA+TGG - chr5.4:46186660-46186679 MS.gene94080:CDS 25.0%
AAAGGAGTTAACATAAGCTA+TGG - chr5.4:46186615-46186634 MS.gene94080:CDS 30.0%
ATAACAACTTTGCTTGACAA+TGG + chr5.4:46186644-46186663 None:intergenic 30.0%
CTACATTGATGAAAAGTGAA+CGG + chr5.4:46186174-46186193 None:intergenic 30.0%
TATTGACATAGACATGATCA+TGG - chr5.4:46185300-46185319 MS.gene94080:CDS 30.0%
TGGGTTTCCTATGAATAAAT+GGG - chr5.4:46186461-46186480 MS.gene94080:CDS 30.0%
TTGTCAAGCAAAGTTGTTAT+TGG - chr5.4:46186644-46186663 MS.gene94080:CDS 30.0%
! TGGAGAATTCTAGAAAATCT+GGG + chr5.4:46185333-46185352 None:intergenic 30.0%
! TGTCAAGCAAAGTTGTTATT+GGG - chr5.4:46186645-46186664 MS.gene94080:CDS 30.0%
! TTGGAGAATTCTAGAAAATC+TGG + chr5.4:46185334-46185353 None:intergenic 30.0%
!!! TTAGTGGAGGAAGTATTTTT+AGG - chr5.4:46186492-46186511 MS.gene94080:CDS 30.0%
AAATGAGCAAACTTGACGTA+AGG + chr5.4:46185889-46185908 None:intergenic 35.0%
AAGCAATAGAATCTGAAGAC+CGG + chr5.4:46186296-46186315 None:intergenic 35.0%
AAGCTGTTACTCATGAAAGA+AGG - chr5.4:46185932-46185951 MS.gene94080:CDS 35.0%
AGAGTTGGGAAACAATAAGT+TGG - chr5.4:46186695-46186714 MS.gene94080:CDS 35.0%
AGCTGTTACTCATGAAAGAA+GGG - chr5.4:46185933-46185952 MS.gene94080:CDS 35.0%
ATTTAACGATGGGTATAGAG+TGG - chr5.4:46186671-46186690 MS.gene94080:CDS 35.0%
ATTTGCCACATCTTAGTTAC+CGG - chr5.4:46186274-46186293 MS.gene94080:CDS 35.0%
CAAGATGAAGAAGAAGAAGA+TGG - chr5.4:46185526-46185545 MS.gene94080:CDS 35.0%
CTGGGTTTCCTATGAATAAA+TGG - chr5.4:46186460-46186479 MS.gene94080:CDS 35.0%
GAACATGGAGATTAATGACA+TGG - chr5.4:46185276-46185295 MS.gene94080:CDS 35.0%
TCTATTGCTTCCTTCTTGAA+TGG - chr5.4:46186306-46186325 MS.gene94080:CDS 35.0%
TGAGAGAATCAATGAAGTTG+TGG + chr5.4:46185453-46185472 None:intergenic 35.0%
TGCTCCAAATCTAGATAACA+AGG - chr5.4:46185495-46185514 MS.gene94080:CDS 35.0%
TGGACTTTGATATCATGGAA+GGG - chr5.4:46185965-46185984 MS.gene94080:CDS 35.0%
TTGTCTAGAAACAAAGAACG+TGG - chr5.4:46186066-46186085 MS.gene94080:CDS 35.0%
TTTCATGAGTAACAGCTTCA+AGG + chr5.4:46185930-46185949 None:intergenic 35.0%
! ACGAACAAGCTTTAATGAAG+AGG + chr5.4:46186223-46186242 None:intergenic 35.0%
! CACTTTTCATCAATGTAGGT+TGG - chr5.4:46186176-46186195 MS.gene94080:CDS 35.0%
! GCAACCTTGTTATCTAGATT+TGG + chr5.4:46185502-46185521 None:intergenic 35.0%
! GTTATCTAGATTTGGAGCAA+CGG + chr5.4:46185494-46185513 None:intergenic 35.0%
! TCTTCATTAAAGCTTGTTCG+TGG - chr5.4:46186222-46186241 MS.gene94080:CDS 35.0%
! TGGCTCAAGTGATATTGATT+CGG - chr5.4:46185683-46185702 MS.gene94080:CDS 35.0%
! TTATCTAGATTTGGAGCAAC+GGG + chr5.4:46185493-46185512 None:intergenic 35.0%
! TTCCTATGAATAAATGGGCT+AGG - chr5.4:46186466-46186485 MS.gene94080:CDS 35.0%
!! ACAATAAGTTGGTGTTAGGT+TGG - chr5.4:46186706-46186725 MS.gene94080:CDS 35.0%
!! ATTTTTAGGCCCACGAATAA+TGG - chr5.4:46186506-46186525 MS.gene94080:CDS 35.0%
!! CATAGTGGACTTTGATATCA+TGG - chr5.4:46185960-46185979 MS.gene94080:CDS 35.0%
!! CGTTCACTTTTCATCAATGT+AGG - chr5.4:46186172-46186191 MS.gene94080:CDS 35.0%
!! GGAAACAATAAGTTGGTGTT+AGG - chr5.4:46186702-46186721 MS.gene94080:CDS 35.0%
!! TGTTAGGTTGGAAATCAAGA+CGG - chr5.4:46186718-46186737 MS.gene94080:CDS 35.0%
!! TTTTTAGGCCCACGAATAAT+GGG - chr5.4:46186507-46186526 MS.gene94080:CDS 35.0%
AAAAGTGAACGGTTGTCCAA+CGG + chr5.4:46186163-46186182 None:intergenic 40.0%
AAGAAATCCAAGTCAAGGAG+AGG + chr5.4:46185409-46185428 None:intergenic 40.0%
AGAAACAAAGAACGTGGCAA+TGG - chr5.4:46186072-46186091 MS.gene94080:CDS 40.0%
AGAAATCCAAGTCAAGGAGA+GGG + chr5.4:46185408-46185427 None:intergenic 40.0%
AGAAGAAGAAGATGGATCCA+TGG - chr5.4:46185534-46185553 MS.gene94080:CDS 40.0%
AGTTTCTTGAACAGTGGCAA+AGG + chr5.4:46186112-46186131 None:intergenic 40.0%
ATTGAATGAGTGAAGCCCAT+TGG + chr5.4:46185993-46186012 None:intergenic 40.0%
CATGTCTTGAAGAAGCTGAA+AGG + chr5.4:46185863-46185882 None:intergenic 40.0%
CTCCTAGCCCATTTATTCAT+AGG + chr5.4:46186471-46186490 None:intergenic 40.0%
CTGTTCAAGAAACTGGAAGA+AGG - chr5.4:46186118-46186137 MS.gene94080:CDS 40.0%
GAAGAAGAAGATGGATCCAT+GGG - chr5.4:46185535-46185554 MS.gene94080:CDS 40.0%
GGACTTTGATATCATGGAAG+GGG - chr5.4:46185966-46185985 MS.gene94080:CDS 40.0%
GTCCAACTCAAATTCCACAA+CGG - chr5.4:46185564-46185583 MS.gene94080:CDS 40.0%
GTGGACTTTGATATCATGGA+AGG - chr5.4:46185964-46185983 MS.gene94080:CDS 40.0%
TAACAGCTTCAAGGATAGCT+TGG + chr5.4:46185921-46185940 None:intergenic 40.0%
TCAATATCACTTGAGCCAGA+TGG + chr5.4:46185681-46185700 None:intergenic 40.0%
TCACTTTCATCGCGAAAAGA+AGG - chr5.4:46186012-46186031 MS.gene94080:CDS 40.0%
TCTTCCAGTTTCTTGAACAG+TGG + chr5.4:46186118-46186137 None:intergenic 40.0%
TGAAAGAAGGGTTCACATAG+TGG - chr5.4:46185945-46185964 MS.gene94080:CDS 40.0%
TGAAGTTGTGGAAGTCATCT+TGG + chr5.4:46185441-46185460 None:intergenic 40.0%
TGGTGAAGAAATCCAAGTCA+AGG + chr5.4:46185414-46185433 None:intergenic 40.0%
TTGAATGAGTGAAGCCCATT+GGG + chr5.4:46185992-46186011 None:intergenic 40.0%
TTTGCCACTGTTCAAGAAAC+TGG - chr5.4:46186111-46186130 MS.gene94080:CDS 40.0%
! ACTCTTGTTTGTGCCTGTTA+GGG + chr5.4:46185656-46185675 None:intergenic 40.0%
! ATTAAAGCTTGTTCGTGGAG+AGG - chr5.4:46186227-46186246 MS.gene94080:CDS 40.0%
! CGTCAAGTTTGCTCATTTCA+CGG - chr5.4:46185891-46185910 MS.gene94080:CDS 40.0%
! TGAGAGTATGTCTGTTTGGT+GGG + chr5.4:46185839-46185858 None:intergenic 40.0%
! TTCACCAGTCTTGTACAACT+GGG + chr5.4:46186541-46186560 None:intergenic 40.0%
!! AGTGATATTGATTCGGCTCA+AGG - chr5.4:46185690-46185709 MS.gene94080:CDS 40.0%
!! TAAATGGGCTAGGAGTTTAG+TGG - chr5.4:46186476-46186495 MS.gene94080:CDS 40.0%
AAAGCTCGTGACATTAGTCG+AGG - chr5.4:46186347-46186366 MS.gene94080:CDS 45.0%
AATGCAGTGATGCGAAGATG+TGG + chr5.4:46186048-46186067 None:intergenic 45.0%
ACAAAGCCTGCATCGTTGAT+GGG + chr5.4:46186384-46186403 None:intergenic 45.0%
ACATTAGTCGAGGAGGAAGT+AGG - chr5.4:46186357-46186376 MS.gene94080:CDS 45.0%
ATGCAGTGATGCGAAGATGT+GGG + chr5.4:46186047-46186066 None:intergenic 45.0%
CAACTCAAATTCCACAACGG+AGG - chr5.4:46185567-46185586 MS.gene94080:CDS 45.0%
CACAAACAAGAGTCACCATC+TGG - chr5.4:46185663-46185682 MS.gene94080:CDS 45.0%
CACAAACTCTGTCTTCTCAC+TGG - chr5.4:46185811-46185830 MS.gene94080:CDS 45.0%
CAGTGAGAAGACAGAGTTTG+TGG + chr5.4:46185813-46185832 None:intergenic 45.0%
CATTAGTCGAGGAGGAAGTA+GGG - chr5.4:46186358-46186377 MS.gene94080:CDS 45.0%
CTCCGTTGTGGAATTTGAGT+TGG + chr5.4:46185569-46185588 None:intergenic 45.0%
GAAATCCAAGTCAAGGAGAG+GGG + chr5.4:46185407-46185426 None:intergenic 45.0%
GAAGACCGGTAACTAAGATG+TGG + chr5.4:46186282-46186301 None:intergenic 45.0%
GGGTATAGAGTGGAAGAGTT+GGG - chr5.4:46186681-46186700 MS.gene94080:CDS 45.0%
GTTGTACAAGACTGGTGAAG+AGG - chr5.4:46186542-46186561 MS.gene94080:CDS 45.0%
GTTGTGGAAGTCATCTTGGT+TGG + chr5.4:46185437-46185456 None:intergenic 45.0%
TCCGTTTAGCAGAGTTTGGA+GGG + chr5.4:46185776-46185795 None:intergenic 45.0%
TGGGTATAGAGTGGAAGAGT+TGG - chr5.4:46186680-46186699 MS.gene94080:CDS 45.0%
TTCCGTTTAGCAGAGTTTGG+AGG + chr5.4:46185777-46185796 None:intergenic 45.0%
! AGGCTGAGAGTATGTCTGTT+TGG + chr5.4:46185843-46185862 None:intergenic 45.0%
! AGGCTTTGTGGGTTTGTTCA+TGG - chr5.4:46186395-46186414 MS.gene94080:CDS 45.0%
! ATCAACGATGCAGGCTTTGT+GGG - chr5.4:46186384-46186403 MS.gene94080:CDS 45.0%
! CTGAGAGTATGTCTGTTTGG+TGG + chr5.4:46185840-46185859 None:intergenic 45.0%
! CTTCACCAGTCTTGTACAAC+TGG + chr5.4:46186542-46186561 None:intergenic 45.0%
! GACTCTTGTTTGTGCCTGTT+AGG + chr5.4:46185657-46185676 None:intergenic 45.0%
! GAGAGTATGTCTGTTTGGTG+GGG + chr5.4:46185838-46185857 None:intergenic 45.0%
! GGTGGTAGTGTTACTGTGAT+TGG + chr5.4:46185353-46185372 None:intergenic 45.0%
! GGTTGCTTTCTGCTTCTGTT+TGG - chr5.4:46186739-46186758 MS.gene94080:CDS 45.0%
! TTCCTTCTTGAATGGTGCCA+AGG - chr5.4:46186314-46186333 MS.gene94080:CDS 45.0%
AAATTCCACAACGGAGGAGG+AGG - chr5.4:46185573-46185592 MS.gene94080:CDS 50.0%
AACGGGTGTGATTTCGTCGT+TGG + chr5.4:46185476-46185495 None:intergenic 50.0%
AGCAAGAGAGGTTCTCTTGG+GGG - chr5.4:46186568-46186587 MS.gene94080:CDS 50.0%
AGCGATGTATGACTCGTTCG+AGG - chr5.4:46186437-46186456 MS.gene94080:CDS 50.0%
AGGAGCAAGAGAGGTTCTCT+TGG - chr5.4:46186565-46186584 MS.gene94080:CDS 50.0%
AGTGGCGTCATCATCAGAAG+TGG + chr5.4:46185380-46185399 None:intergenic 50.0%
ATATCATGGAAGGGGCCCAA+TGG - chr5.4:46185974-46185993 MS.gene94080:CDS 50.0%
ATGTATGACTCGTTCGAGGC+TGG - chr5.4:46186441-46186460 MS.gene94080:CDS 50.0%
CACAAAGCCTGCATCGTTGA+TGG + chr5.4:46186385-46186404 None:intergenic 50.0%
CATCAACGATGCAGGCTTTG+TGG - chr5.4:46186383-46186402 MS.gene94080:CDS 50.0%
CCACTGAGCCCATTATTCGT+GGG + chr5.4:46186518-46186537 None:intergenic 50.0%
CGGTTCCGTTTAGCAGAGTT+TGG + chr5.4:46185780-46185799 None:intergenic 50.0%
CGTCATCATCAGAAGTGGGT+GGG + chr5.4:46185375-46185394 None:intergenic 50.0%
CTCAAATTCCACAACGGAGG+AGG - chr5.4:46185570-46185589 MS.gene94080:CDS 50.0%
CTCCTTGGCACCATTCAAGA+AGG + chr5.4:46186319-46186338 None:intergenic 50.0%
GACTTAGTGTCCTCCACTCA+CGG - chr5.4:46185712-46185731 MS.gene94080:CDS 50.0%
GAGCAAGAGAGGTTCTCTTG+GGG - chr5.4:46186567-46186586 MS.gene94080:CDS 50.0%
GAGGAGGAGGATGACAGAAA+GGG - chr5.4:46185592-46185611 MS.gene94080:CDS 50.0%
GAGTTTGTGGTGACCACAGT+CGG + chr5.4:46185800-46185819 None:intergenic 50.0%
GCCCTCCAAACTCTGCTAAA+CGG - chr5.4:46185772-46185791 MS.gene94080:CDS 50.0%
GGAGCAAGAGAGGTTCTCTT+GGG - chr5.4:46186566-46186585 MS.gene94080:CDS 50.0%
GTACAAGACTGGTGAAGAGG+AGG - chr5.4:46186545-46186564 MS.gene94080:CDS 50.0%
GTCATCATCAGAAGTGGGTG+GGG + chr5.4:46185374-46185393 None:intergenic 50.0%
GTGGAAGTCATCTTGGTTGG+TGG + chr5.4:46185434-46185453 None:intergenic 50.0%
GTGGCGTCATCATCAGAAGT+GGG + chr5.4:46185379-46185398 None:intergenic 50.0%
TATCATGGAAGGGGCCCAAT+GGG - chr5.4:46185975-46185994 MS.gene94080:CDS 50.0%
TCTAGTGCACCTCCTTATGG+CGG - chr5.4:46185621-46185640 MS.gene94080:CDS 50.0%
TGTATGACTCGTTCGAGGCT+GGG - chr5.4:46186442-46186461 MS.gene94080:CDS 50.0%
TTGGGTGTGGAAGGGTTCAA+AGG - chr5.4:46186597-46186616 MS.gene94080:CDS 50.0%
TTGTCCAACGGAAGCAGCAA+AGG + chr5.4:46186151-46186170 None:intergenic 50.0%
! ACAACCTTTGCTGCTTCCGT+TGG - chr5.4:46186144-46186163 MS.gene94080:CDS 50.0%
! CCATAAGGAGGTGCACTAGA+CGG + chr5.4:46185621-46185640 None:intergenic 50.0%
! CTTCTGATGATGACGCCACT+TGG - chr5.4:46185380-46185399 MS.gene94080:CDS 50.0%
! TGAGTTGGACATGTCTCCCA+TGG + chr5.4:46185554-46185573 None:intergenic 50.0%
! TTGAATGGTGCCAAGGAGCT+AGG - chr5.4:46186321-46186340 MS.gene94080:CDS 50.0%
!! ATGGGCTAGGAGTTTAGTGG+AGG - chr5.4:46186479-46186498 MS.gene94080:CDS 50.0%
!! GAGCTTTGTTCCTAGCTCCT+TGG + chr5.4:46186334-46186353 None:intergenic 50.0%
ATCATCAGAAGTGGGTGGGG+TGG + chr5.4:46185371-46185390 None:intergenic 55.0%
ATCGCGAAAAGAAGGCCTGC+CGG - chr5.4:46186020-46186039 MS.gene94080:CDS 55.0%
CCGTCTAGTGCACCTCCTTA+TGG - chr5.4:46185618-46185637 MS.gene94080:CDS 55.0%
CTGCTAAACGGAACCGACTG+TGG - chr5.4:46185784-46185803 MS.gene94080:CDS 55.0%
GCAAGAGAGGTTCTCTTGGG+GGG - chr5.4:46186569-46186588 MS.gene94080:CDS 55.0%
GCCACTGAGCCCATTATTCG+TGG + chr5.4:46186519-46186538 None:intergenic 55.0%
GCGTCATCATCAGAAGTGGG+TGG + chr5.4:46185376-46185395 None:intergenic 55.0%
GCTCGTGACATTAGTCGAGG+AGG - chr5.4:46186350-46186369 MS.gene94080:CDS 55.0%
GCTTGTTCGTGGAGAGGCTT+TGG - chr5.4:46186233-46186252 MS.gene94080:CDS 55.0%
GGAGGAGGAGGATGACAGAA+AGG - chr5.4:46185591-46185610 MS.gene94080:CDS 55.0%
GTGAAGAGGAGGAGCAAGAG+AGG - chr5.4:46186556-46186575 MS.gene94080:CDS 55.0%
GTGGCCCAGTTGTACAAGAC+TGG - chr5.4:46186534-46186553 MS.gene94080:CDS 55.0%
TTCTCTTGGGGGGAGTGGTT+GGG - chr5.4:46186579-46186598 MS.gene94080:CDS 55.0%
TTGGTCCCCTCTCCTTGACT+TGG - chr5.4:46185399-46185418 MS.gene94080:CDS 55.0%
! TAAGGAGGTGCACTAGACGG+AGG + chr5.4:46185618-46185637 None:intergenic 55.0%
! TAGCAGAGTTTGGAGGGCGT+CGG + chr5.4:46185770-46185789 None:intergenic 55.0%
!! CCCACGAATAATGGGCTCAG+TGG - chr5.4:46186515-46186534 MS.gene94080:CDS 55.0%
AGAGGTTCTCTTGGGGGGAG+TGG - chr5.4:46186574-46186593 MS.gene94080:CDS 60.0%
CGCAGCGAGTCTCTCCATGT+TGG + chr5.4:46185740-46185759 None:intergenic 60.0%
CTCCACTCACGGCACCAACA+TGG - chr5.4:46185723-46185742 MS.gene94080:CDS 60.0%
GGGAGTGGTTGGGTGTGGAA+GGG - chr5.4:46186589-46186608 MS.gene94080:CDS 60.0%
GTAGGGCCCATCAACGATGC+AGG - chr5.4:46186375-46186394 MS.gene94080:CDS 60.0%
GTCAAGGAGAGGGGACCAAG+TGG + chr5.4:46185398-46185417 None:intergenic 60.0%
GTGATGCGAAGATGTGGGCC+CGG + chr5.4:46186042-46186061 None:intergenic 60.0%
GTTCTCTTGGGGGGAGTGGT+TGG - chr5.4:46186578-46186597 MS.gene94080:CDS 60.0%
TCGCGAAAAGAAGGCCTGCC+GGG - chr5.4:46186021-46186040 MS.gene94080:CDS 60.0%
TTCCACAACGGAGGAGGAGG+AGG - chr5.4:46185576-46185595 MS.gene94080:CDS 60.0%
!! CATGTTGGTGCCGTGAGTGG+AGG + chr5.4:46185725-46185744 None:intergenic 60.0%
!! CTCCATGTTGGTGCCGTGAG+TGG + chr5.4:46185728-46185747 None:intergenic 60.0%
CACAACGGAGGAGGAGGAGG+AGG - chr5.4:46185579-46185598 MS.gene94080:CDS 65.0%
CTCCTCCTCCTCCTCCGTTG+TGG + chr5.4:46185581-46185600 None:intergenic 65.0%
GCACCTCCTTATGGCGGCAG+CGG - chr5.4:46185627-46185646 MS.gene94080:CDS 65.0%
GGGGAGTGGTTGGGTGTGGA+AGG - chr5.4:46186588-46186607 MS.gene94080:CDS 65.0%
TTGGGGGGAGTGGTTGGGTG+TGG - chr5.4:46186584-46186603 MS.gene94080:CDS 65.0%
CCTCCGCTGCCGCCATAAGG+AGG + chr5.4:46185633-46185652 None:intergenic 70.0%
CCTCCTTATGGCGGCAGCGG+AGG - chr5.4:46185630-46185649 MS.gene94080:CDS 70.0%
TGCGAAGATGTGGGCCCGGC+AGG + chr5.4:46186038-46186057 None:intergenic 70.0%
GCGGCAGCGGAGGCCCTAAC+AGG - chr5.4:46185640-46185659 MS.gene94080:CDS 75.0%
GGGCCTCCGCTGCCGCCATA+AGG + chr5.4:46185636-46185655 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 46185268 46186794 46185268 ID=MS.gene94080
chr5.4 mRNA 46185268 46186794 46185268 ID=MS.gene94080.t1;Parent=MS.gene94080
chr5.4 exon 46185268 46186794 46185268 ID=MS.gene94080.t1.exon1;Parent=MS.gene94080.t1
chr5.4 CDS 46185268 46186794 46185268 ID=cds.MS.gene94080.t1;Parent=MS.gene94080.t1
Gene Sequence

>MS.gene94080

ATGATCATGAACATGGAGATTAATGACATGGATATTGACATAGACATGATCATGGATGTTGTCCCAGATTTTCTAGAATTCTCCAATCACAGTAACACTACCACCCCACCCACTTCTGATGATGACGCCACTTGGTCCCCTCTCCTTGACTTGGATTTCTTCACCACCAACCAAGATGACTTCCACAACTTCATTGATTCTCTCACCAACGACGAAATCACACCCGTTGCTCCAAATCTAGATAACAAGGTTGCAAAGCAAGATGAAGAAGAAGAAGATGGATCCATGGGAGACATGTCCAACTCAAATTCCACAACGGAGGAGGAGGAGGAGGATGACAGAAAGGGCCTCCGTCTAGTGCACCTCCTTATGGCGGCAGCGGAGGCCCTAACAGGCACAAACAAGAGTCACCATCTGGCTCAAGTGATATTGATTCGGCTCAAGGACTTAGTGTCCTCCACTCACGGCACCAACATGGAGAGACTCGCTGCGTATTTTACCGACGCCCTCCAAACTCTGCTAAACGGAACCGACTGTGGTCACCACAAACTCTGTCTTCTCACTGGACCCCACCAAACAGACATACTCTCAGCCTTTCAGCTTCTTCAAGACATGTCTCCTTACGTCAAGTTTGCTCATTTCACGGCAAACCAAGCTATCCTTGAAGCTGTTACTCATGAAAGAAGGGTTCACATAGTGGACTTTGATATCATGGAAGGGGCCCAATGGGCTTCACTCATTCAATCACTTTCATCGCGAAAAGAAGGCCTGCCGGGCCCACATCTTCGCATCACTGCATTGTCTAGAAACAAAGAACGTGGCAATGGACGATCTAGATCGTCCTTTGCCACTGTTCAAGAAACTGGAAGAAGGTTAACAACCTTTGCTGCTTCCGTTGGACAACCGTTCACTTTTCATCAATGTAGGTTGGAATCAGACGAAACGTTTCGAACCTCTTCATTAAAGCTTGTTCGTGGAGAGGCTTTGGTGTTCAACTGTGTCATGCATTTGCCACATCTTAGTTACCGGTCTTCAGATTCTATTGCTTCCTTCTTGAATGGTGCCAAGGAGCTAGGAACAAAGCTCGTGACATTAGTCGAGGAGGAAGTAGGGCCCATCAACGATGCAGGCTTTGTGGGTTTGTTCATGGATTCATTGCATCGTTATTCAGCGATGTATGACTCGTTCGAGGCTGGGTTTCCTATGAATAAATGGGCTAGGAGTTTAGTGGAGGAAGTATTTTTAGGCCCACGAATAATGGGCTCAGTGGCCCAGTTGTACAAGACTGGTGAAGAGGAGGAGCAAGAGAGGTTCTCTTGGGGGGAGTGGTTGGGTGTGGAAGGGTTCAAAGGAGTTAACATAAGCTATGGTAACCATTGTCAAGCAAAGTTGTTATTGGGTTTATTTAACGATGGGTATAGAGTGGAAGAGTTGGGAAACAATAAGTTGGTGTTAGGTTGGAAATCAAGACGGTTGCTTTCTGCTTCTGTTTGGACTTGTTTGTCAGAGATTGAATATGATTTTTAA
Protein sequence

>MS.gene94080.t1

MIMNMEINDMDIDIDMIMDVVPDFLEFSNHSNTTTPPTSDDDATWSPLLDLDFFTTNQDDFHNFIDSLTNDEITPVAPNLDNKVAKQDEEEEDGSMGDMSNSNSTTEEEEEDDRKGLRLVHLLMAAAEALTGTNKSHHLAQVILIRLKDLVSSTHGTNMERLAAYFTDALQTLLNGTDCGHHKLCLLTGPHQTDILSAFQLLQDMSPYVKFAHFTANQAILEAVTHERRVHIVDFDIMEGAQWASLIQSLSSRKEGLPGPHLRITALSRNKERGNGRSRSSFATVQETGRRLTTFAASVGQPFTFHQCRLESDETFRTSSLKLVRGEALVFNCVMHLPHLSYRSSDSIASFLNGAKELGTKLVTLVEEEVGPINDAGFVGLFMDSLHRYSAMYDSFEAGFPMNKWARSLVEEVFLGPRIMGSVAQLYKTGEEEEQERFSWGEWLGVEGFKGVNISYGNHCQAKLLLGLFNDGYRVEELGNNKLVLGWKSRRLLSASVWTCLSEIEYDF