Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94343.t1 | XP_024640623.1 | 93.7 | 205 | 13 | 0 | 1 | 205 | 1 | 205 | 5.00E-104 | 387.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94343.t1 | Q9SZL8 | 27.3 | 187 | 119 | 7 | 30 | 204 | 59 | 240 | 1.8e-09 | 64.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94343.t1 | A0A072TDR2 | 94.3 | 158 | 9 | 0 | 20 | 177 | 1 | 158 | 2.8e-80 | 307.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene94343.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94343.t1 | MTR_0853s0010 | 94.304 | 158 | 9 | 0 | 20 | 177 | 1 | 158 | 3.89e-111 | 315 |
| MS.gene94343.t1 | MTR_1g019850 | 52.381 | 168 | 67 | 2 | 18 | 179 | 38 | 198 | 2.02e-53 | 171 |
| MS.gene94343.t1 | MTR_2g024230 | 55.224 | 67 | 30 | 0 | 37 | 103 | 83 | 149 | 4.70e-21 | 85.9 |
| MS.gene94343.t1 | MTR_1g033820 | 44.000 | 75 | 41 | 1 | 32 | 105 | 15 | 89 | 2.47e-15 | 68.9 |
| MS.gene94343.t1 | MTR_6g048540 | 48.485 | 66 | 32 | 1 | 35 | 98 | 19 | 84 | 1.22e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94343.t1 | AT4G38180 | 27.273 | 176 | 111 | 6 | 41 | 204 | 70 | 240 | 6.07e-12 | 64.3 |
Find 25 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTCGGCATATGATTT+TGG | 0.142338 | 3.2:-43149051 | None:intergenic |
| TATTGATCCTTGTATTGATA+TGG | 0.239238 | 3.2:+43149290 | MS.gene94343:CDS |
| CCAAGAGTATAAACTATCTT+AGG | 0.313740 | 3.2:+43149613 | MS.gene94343:CDS |
| AAATGTAATGATAGGCCATT+TGG | 0.319534 | 3.2:-43149559 | None:intergenic |
| CCTAAGATAGTTTATACTCT+TGG | 0.340102 | 3.2:-43149613 | None:intergenic |
| CTAAGATAGTTTATACTCTT+GGG | 0.346829 | 3.2:-43149612 | None:intergenic |
| CATGTTATGACTATCTTCTT+CGG | 0.402279 | 3.2:-43149064 | None:intergenic |
| AACATCAAGAACTGATTGTA+AGG | 0.444776 | 3.2:+43149503 | MS.gene94343:CDS |
| ATATTTGTTTGCTCTAATGC+TGG | 0.447741 | 3.2:+43149411 | MS.gene94343:CDS |
| AATGTAATGATAGGCCATTT+GGG | 0.467781 | 3.2:-43149558 | None:intergenic |
| AATGTTTAGCAATGAAGGTT+CGG | 0.471515 | 3.2:+43149719 | MS.gene94343:CDS |
| TGAACATCTAAGTCATGCTC+AGG | 0.476339 | 3.2:-43149255 | None:intergenic |
| GATCAAGAGGGCTTGCCTAC+CGG | 0.495549 | 3.2:+43149684 | MS.gene94343:CDS |
| GCTGCAATGTTTAGCAATGA+AGG | 0.497956 | 3.2:+43149714 | MS.gene94343:CDS |
| GTATTGTTAAATGTAATGAT+AGG | 0.499697 | 3.2:-43149567 | None:intergenic |
| TGTCATTACTATCAACATGT+TGG | 0.533945 | 3.2:-43149434 | None:intergenic |
| TCATAACATGGAGGACATAG+AGG | 0.553789 | 3.2:+43149077 | MS.gene94343:CDS |
| AGTTGAACAGTTTGATCAAG+AGG | 0.557965 | 3.2:+43149671 | MS.gene94343:CDS |
| TAAGATAGTTTATACTCTTG+GGG | 0.560800 | 3.2:-43149611 | None:intergenic |
| GTTGAACAGTTTGATCAAGA+GGG | 0.578599 | 3.2:+43149672 | MS.gene94343:CDS |
| CGAAGAAGATAGTCATAACA+TGG | 0.604552 | 3.2:+43149065 | MS.gene94343:CDS |
| CAATACTCATAATCATGCAA+TGG | 0.611156 | 3.2:+43149584 | MS.gene94343:CDS |
| TCAAATTCCATATCAATACA+AGG | 0.614636 | 3.2:-43149297 | None:intergenic |
| GAACATCTAAGTCATGCTCA+GGG | 0.664893 | 3.2:-43149254 | None:intergenic |
| AGAAGATAGTCATAACATGG+AGG | 0.736903 | 3.2:+43149068 | MS.gene94343:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTATTGTTAAATGTAATGAT+AGG | - | chr3.2:43149570-43149589 | None:intergenic | 20.0% |
| ! | CTAAGATAGTTTATACTCTT+GGG | - | chr3.2:43149615-43149634 | None:intergenic | 25.0% |
| ! | TAAGATAGTTTATACTCTTG+GGG | - | chr3.2:43149614-43149633 | None:intergenic | 25.0% |
| ! | TAGTTTCAAAAGCAAAAATG+AGG | + | chr3.2:43149535-43149554 | MS.gene94343:CDS | 25.0% |
| ! | TCAAATTCCATATCAATACA+AGG | - | chr3.2:43149300-43149319 | None:intergenic | 25.0% |
| !! | TATTGATCCTTGTATTGATA+TGG | + | chr3.2:43149290-43149309 | MS.gene94343:CDS | 25.0% |
| !! | TTTGCTAAAAAAGAAGGTTT+TGG | + | chr3.2:43149354-43149373 | MS.gene94343:CDS | 25.0% |
| AAATGTAATGATAGGCCATT+TGG | - | chr3.2:43149562-43149581 | None:intergenic | 30.0% | |
| AACATCAAGAACTGATTGTA+AGG | + | chr3.2:43149503-43149522 | MS.gene94343:CDS | 30.0% | |
| AATGTAATGATAGGCCATTT+GGG | - | chr3.2:43149561-43149580 | None:intergenic | 30.0% | |
| AATGTTTAGCAATGAAGGTT+CGG | + | chr3.2:43149719-43149738 | MS.gene94343:CDS | 30.0% | |
| ATATTTGTTTGCTCTAATGC+TGG | + | chr3.2:43149411-43149430 | MS.gene94343:CDS | 30.0% | |
| CAATACTCATAATCATGCAA+TGG | + | chr3.2:43149584-43149603 | MS.gene94343:CDS | 30.0% | |
| CATGTTATGACTATCTTCTT+CGG | - | chr3.2:43149067-43149086 | None:intergenic | 30.0% | |
| CCTAAGATAGTTTATACTCT+TGG | - | chr3.2:43149616-43149635 | None:intergenic | 30.0% | |
| TGTCATTACTATCAACATGT+TGG | - | chr3.2:43149437-43149456 | None:intergenic | 30.0% | |
| ! | ACGTCATTTGCTAAAAAAGA+AGG | + | chr3.2:43149348-43149367 | MS.gene94343:CDS | 30.0% |
| ! | CCAAGAGTATAAACTATCTT+AGG | + | chr3.2:43149613-43149632 | MS.gene94343:CDS | 30.0% |
| ! | CTTAGGTGCCATAAAAAAAT+GGG | + | chr3.2:43149630-43149649 | MS.gene94343:CDS | 30.0% |
| ! | CTTTCTCTGATAGAGATTTT+TGG | + | chr3.2:43149742-43149761 | MS.gene94343:CDS | 30.0% |
| ! | TCTTAGGTGCCATAAAAAAA+TGG | + | chr3.2:43149629-43149648 | MS.gene94343:CDS | 30.0% |
| AGAAGATAGTCATAACATGG+AGG | + | chr3.2:43149068-43149087 | MS.gene94343:CDS | 35.0% | |
| AGTTGAACAGTTTGATCAAG+AGG | + | chr3.2:43149671-43149690 | MS.gene94343:CDS | 35.0% | |
| CGAAGAAGATAGTCATAACA+TGG | + | chr3.2:43149065-43149084 | MS.gene94343:CDS | 35.0% | |
| GTTGAACAGTTTGATCAAGA+GGG | + | chr3.2:43149672-43149691 | MS.gene94343:CDS | 35.0% | |
| ! | TCTTCTTCGGCATATGATTT+TGG | - | chr3.2:43149054-43149073 | None:intergenic | 35.0% |
| AAGCAAAAATGAGGCCCAAA+TGG | + | chr3.2:43149544-43149563 | MS.gene94343:CDS | 40.0% | |
| GAACATCTAAGTCATGCTCA+GGG | - | chr3.2:43149257-43149276 | None:intergenic | 40.0% | |
| GCTGCAATGTTTAGCAATGA+AGG | + | chr3.2:43149714-43149733 | MS.gene94343:CDS | 40.0% | |
| GTGCCATAAAAAAATGGGTG+GGG | + | chr3.2:43149635-43149654 | MS.gene94343:CDS | 40.0% | |
| TCATAACATGGAGGACATAG+AGG | + | chr3.2:43149077-43149096 | MS.gene94343:CDS | 40.0% | |
| TGAACATCTAAGTCATGCTC+AGG | - | chr3.2:43149258-43149277 | None:intergenic | 40.0% | |
| ! | AAACATTGCAGCGACTTTTC+CGG | - | chr3.2:43149706-43149725 | None:intergenic | 40.0% |
| ! | AGGTGCCATAAAAAAATGGG+TGG | + | chr3.2:43149633-43149652 | MS.gene94343:CDS | 40.0% |
| ! | GGTGCCATAAAAAAATGGGT+GGG | + | chr3.2:43149634-43149653 | MS.gene94343:CDS | 40.0% |
| !!! | CTGCCCCACCCATTTTTTTA+TGG | - | chr3.2:43149641-43149660 | None:intergenic | 45.0% |
| ! | ATTGCAGCGACTTTTCCGGT+AGG | - | chr3.2:43149702-43149721 | None:intergenic | 50.0% |
| GATCAAGAGGGCTTGCCTAC+CGG | + | chr3.2:43149684-43149703 | MS.gene94343:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 43149042 | 43149785 | 43149042 | ID=MS.gene94343 |
| chr3.2 | mRNA | 43149042 | 43149785 | 43149042 | ID=MS.gene94343.t1;Parent=MS.gene94343 |
| chr3.2 | exon | 43149042 | 43149098 | 43149042 | ID=MS.gene94343.t1.exon1;Parent=MS.gene94343.t1 |
| chr3.2 | CDS | 43149042 | 43149098 | 43149042 | ID=cds.MS.gene94343.t1;Parent=MS.gene94343.t1 |
| chr3.2 | exon | 43149225 | 43149785 | 43149225 | ID=MS.gene94343.t1.exon2;Parent=MS.gene94343.t1 |
| chr3.2 | CDS | 43149225 | 43149785 | 43149225 | ID=cds.MS.gene94343.t1;Parent=MS.gene94343.t1 |
| Gene Sequence |
| Protein sequence |
