AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94468

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94468.t1 MTR_7g039520 84.295 312 33 2 13 324 51 346 0.0 525
MS.gene94468.t1 MTR_5g040080 84.615 312 32 2 13 324 51 346 0.0 509
MS.gene94468.t1 MTR_2g014410 74.717 265 36 3 58 322 42 275 9.31e-127 366
MS.gene94468.t1 MTR_1g069015 37.805 82 48 2 15 94 58 138 1.82e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94468.t1 AT1G58210 45.000 80 42 1 15 94 94 171 2.73e-15 75.1

Find 59 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCTCTACAATCTTCTTCT+TGG 0.240672 5.1:-30299742 None:intergenic
TTACCTTCTACCTTCCCTTT+TGG 0.249599 5.1:-30297592 None:intergenic
ATCCTCTACAATCTTCTTCT+TGG 0.277613 5.1:-30299775 None:intergenic
GTGATTTATAATGGTGAAAA+TGG 0.304153 5.1:+30299354 MS.gene94468:CDS
GTGATTTCTAATGGTGAAAA+TGG 0.304848 5.1:+30299564 MS.gene94468:CDS
ACAGGTATGTCGCCGTTTCT+CGG 0.307703 5.1:+30299140 MS.gene94468:intron
CGGTTGGATTTCCACACAAT+TGG 0.311637 5.1:+30299160 MS.gene94468:CDS
GAAAATGTGGATAAAGTTAC+TGG 0.316089 5.1:+30299219 MS.gene94468:CDS
AGTGGAGATCTAACACTATT+TGG 0.318692 5.1:-30299660 None:intergenic
TTCAATTTCTTTGCGTAAAG+TGG 0.326249 5.1:-30299678 None:intergenic
ATAAGTGGTTGAACATGTTT+AGG 0.334891 5.1:+30297555 None:intergenic
AATTATTCAGCTCATGGTTC+GGG 0.340243 5.1:+30299186 MS.gene94468:CDS
AAGTCACACGATGCTTAAAA+AGG 0.345125 5.1:+30299848 MS.gene94468:CDS
GCCTCACGTACGTCTCAAAA+TGG 0.354055 5.1:+30298180 MS.gene94468:CDS
CAAGTTGGCGTGATTTATAA+TGG 0.364333 5.1:+30299345 MS.gene94468:CDS
TCCTGCAAAGTTTCATCAGC+TGG 0.381685 5.1:-30299420 None:intergenic
ACCAGCTGATGAAACTTTGC+AGG 0.388319 5.1:+30299419 MS.gene94468:CDS
CTGGTAATTTGTGTAGTAGT+AGG 0.388874 5.1:+30299799 MS.gene94468:CDS
TGAAACTTTGCAGGAAGATA+TGG 0.392140 5.1:+30299428 MS.gene94468:CDS
AAATTATTCAGCTCATGGTT+CGG 0.408856 5.1:+30299185 MS.gene94468:CDS
GCAATGTTGTCATTGCCTTC+CGG 0.415008 5.1:-30299510 None:intergenic
CAGGTTGACGTGATTTCTAA+TGG 0.433158 5.1:+30299555 MS.gene94468:CDS
GTTGTCTCAACCGGAAGATA+TGG 0.435950 5.1:+30299485 MS.gene94468:CDS
AAGAAGATTGTAGAGGATAC+TGG 0.442437 5.1:+30299780 MS.gene94468:CDS
CCTTCCGGTTCCATATCTTC+CGG 0.446681 5.1:-30299495 None:intergenic
ATCTTGAGTCTCTACAACAT+TGG 0.448912 5.1:-30298124 None:intergenic
GCAATGTTGTCATTACCTTC+AGG 0.449864 5.1:-30299453 None:intergenic
GAAGGTAATGACAACATTGC+GGG 0.450334 5.1:+30299456 MS.gene94468:CDS
TCTTGAGTCTCTACAACATT+GGG 0.460912 5.1:-30298123 None:intergenic
GGGTCTTAAGTTGTCTCAAC+CGG 0.462850 5.1:+30299476 MS.gene94468:CDS
AGCCTGGTAGATCTTCTAAC+AGG 0.463228 5.1:-30299903 None:intergenic
GTATGTCGCCGTTTCTCGGT+TGG 0.465222 5.1:+30299144 MS.gene94468:CDS
ACAACAATATTGCGAGTGTA+AGG 0.490252 5.1:+30299310 MS.gene94468:CDS
TCATGGTTCGGGAAATGAAT+GGG 0.496759 5.1:+30299197 MS.gene94468:CDS
ATCTAACACTATTTGGCTCA+TGG 0.496988 5.1:-30299653 None:intergenic
GTGGAAATCCAACCGAGAAA+CGG 0.509162 5.1:-30299152 None:intergenic
CTCATGGTTCGGGAAATGAA+TGG 0.521512 5.1:+30299196 MS.gene94468:CDS
TGGTAATTTGTGTAGTAGTA+GGG 0.540040 5.1:+30299800 MS.gene94468:CDS
TCTAACACTATTTGGCTCAT+GGG 0.544732 5.1:-30299652 None:intergenic
TCAACCGGAAGATATGGAAC+CGG 0.553131 5.1:+30299491 MS.gene94468:CDS
GGGAAATGAATGGGAAAATG+TGG 0.555111 5.1:+30299206 MS.gene94468:CDS
AGGAGAAGTGAAACATTTCA+CGG 0.569635 5.1:-30299883 None:intergenic
TTATAAATCACGCCAACTTG+TGG 0.575534 5.1:-30299342 None:intergenic
CTCCTGTTAGAAGATCTACC+AGG 0.582686 5.1:+30299901 MS.gene94468:CDS
ACACTATTTGGCTCATGGGT+AGG 0.583997 5.1:-30299648 None:intergenic
CGAATTATCAAGTGACCGCA+AGG 0.593011 5.1:+30299707 MS.gene94468:CDS
AACAGTCGATGAGACTTCAC+AGG 0.601831 5.1:+30299275 MS.gene94468:CDS
TGGTACGTTTCTTATCCTTG+CGG 0.610799 5.1:-30299722 None:intergenic
GGCATCACTGTAATCTTCCG+CGG 0.614423 5.1:+30298147 MS.gene94468:CDS
CAGGAAGATATGGAACCTGA+AGG 0.622434 5.1:+30299438 MS.gene94468:CDS
GAGACTTCACAGGAAGCTGA+AGG 0.645447 5.1:+30299285 MS.gene94468:CDS
AATGTTGTAGAGACTCAAGA+TGG 0.656225 5.1:+30298126 MS.gene94468:CDS
CCGGAAGATATGGAACCGGA+AGG 0.657836 5.1:+30299495 MS.gene94468:CDS
AGGTTGTCTCAACCACAAGT+TGG 0.660440 5.1:+30299330 MS.gene94468:CDS
TACCAAGAAGAAGATTGTAG+AGG 0.672640 5.1:+30299740 MS.gene94468:CDS
TACCAAGAAGAAGATTGTAG+AGG 0.672640 5.1:+30299773 MS.gene94468:CDS
TGAAGGTAATGACAACATTG+CGG 0.680073 5.1:+30299455 MS.gene94468:CDS
TTAGAAATCACGTCAACCTG+CGG 0.710726 5.1:-30299552 None:intergenic
GTGAGGCGTTAATCAAACCG+CGG 0.757884 5.1:-30298164 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATTTTAGAATTAGTTT+TGG + chr5.1:30298605-30298624 MS.gene94468:intron 10.0%
!!! TTTTTTTCATTTTCATTTTT+AGG + chr5.1:30297998-30298017 MS.gene94468:intron 10.0%
!! AAAATACTCATAATCTTCAA+TGG + chr5.1:30299040-30299059 MS.gene94468:intron 20.0%
!! TAATTACTTATTGTGACTAT+TGG + chr5.1:30298045-30298064 MS.gene94468:intron 20.0%
!! TTGAATATAATTAGTGTGTT+TGG + chr5.1:30298298-30298317 MS.gene94468:intron 20.0%
!!! ATCAATCATTCAGTTTTATT+TGG + chr5.1:30298873-30298892 MS.gene94468:intron 20.0%
!!! TATTGACACATTTAATTTTC+TGG + chr5.1:30299098-30299117 MS.gene94468:intron 20.0%
!!! TGTCAAAAAGTATAGATTTT+GGG - chr5.1:30298998-30299017 None:intergenic 20.0%
!!! TTCATTTTTAGGTTTCTAAT+TGG + chr5.1:30298009-30298028 MS.gene94468:intron 20.0%
!!! TTGTCAAAAAGTATAGATTT+TGG - chr5.1:30298999-30299018 None:intergenic 20.0%
!!! TTTTTGTATCAAACTTTCTT+TGG - chr5.1:30298680-30298699 None:intergenic 20.0%
!!! TTTTTTATAATTTAGTAGCC+TGG - chr5.1:30299922-30299941 None:intergenic 20.0%
! AACAAAGCTAGCAAAAATAT+AGG - chr5.1:30298912-30298931 None:intergenic 25.0%
! AAGAGGAATGAAAAAGAAAA+AGG - chr5.1:30298228-30298247 None:intergenic 25.0%
! AATATTCAATGTCTTTCTAC+AGG + chr5.1:30299122-30299141 MS.gene94468:intron 25.0%
! AGAGGAATGAAAAAGAAAAA+GGG - chr5.1:30298227-30298246 None:intergenic 25.0%
! GTGATTTATAATGGTGAAAA+TGG + chr5.1:30299354-30299373 MS.gene94468:CDS 25.0%
! TGATTGATAAACCTCAATTA+TGG - chr5.1:30298861-30298880 None:intergenic 25.0%
! TTCTAATTGGTTATCTGTTT+GGG + chr5.1:30298022-30298041 MS.gene94468:intron 25.0%
! TTTCTAATTGGTTATCTGTT+TGG + chr5.1:30298021-30298040 MS.gene94468:intron 25.0%
!! GAATAATTTTTCCAATTGTG+TGG - chr5.1:30299174-30299193 None:intergenic 25.0%
!! TGTAGATTTTCTTACATGAA+TGG + chr5.1:30297909-30297928 MS.gene94468:intron 25.0%
!! TTGAGAGTACAAATCAAATT+TGG + chr5.1:30298441-30298460 MS.gene94468:intron 25.0%
!!! ATATTTTTGCTAGCTTTGTT+AGG + chr5.1:30298912-30298931 MS.gene94468:intron 25.0%
!!! CAAGTTAGAATTCATTTTTG+TGG + chr5.1:30298486-30298505 MS.gene94468:intron 25.0%
!!! TTTAGATTCACTTTTAAGAG+AGG - chr5.1:30298578-30298597 None:intergenic 25.0%
AAATTATTCAGCTCATGGTT+CGG + chr5.1:30299185-30299204 MS.gene94468:CDS 30.0%
ATTTGAAGATTGAGTTAGAG+AGG - chr5.1:30297794-30297813 None:intergenic 30.0%
GAAAATGTGGATAAAGTTAC+TGG + chr5.1:30299219-30299238 MS.gene94468:CDS 30.0%
GTGATTTCTAATGGTGAAAA+TGG + chr5.1:30299564-30299583 MS.gene94468:CDS 30.0%
TGAAGTAAACACACACAAAA+AGG - chr5.1:30298750-30298769 None:intergenic 30.0%
TGGAAAAATTATTCAGCTCA+TGG + chr5.1:30299180-30299199 MS.gene94468:CDS 30.0%
TGTGTTTACTTCATGTTCAT+GGG + chr5.1:30298757-30298776 MS.gene94468:intron 30.0%
TTCAATTTCTTTGCGTAAAG+TGG - chr5.1:30299681-30299700 None:intergenic 30.0%
TTTGAAGATTGAGTTAGAGA+GGG - chr5.1:30297793-30297812 None:intergenic 30.0%
! AGATTTTCTTACATGAATGG+TGG + chr5.1:30297912-30297931 MS.gene94468:intron 30.0%
! TCAAAATGGTTTTTCTTCTG+AGG + chr5.1:30298194-30298213 MS.gene94468:CDS 30.0%
! TGGTAATTTGTGTAGTAGTA+GGG + chr5.1:30299800-30299819 MS.gene94468:CDS 30.0%
!! TGAACTTTGAAACTATCACT+TGG - chr5.1:30298817-30298836 None:intergenic 30.0%
AAACAAGAGAGAAGAGTGAA+TGG - chr5.1:30297832-30297851 None:intergenic 35.0%
AACAAGAGAGAAGAGTGAAT+GGG - chr5.1:30297831-30297850 None:intergenic 35.0%
AACATGTCAACATTAGTGTC+TGG + chr5.1:30298539-30298558 MS.gene94468:intron 35.0%
AAGAAGATTGTAGAGGATAC+TGG + chr5.1:30299780-30299799 MS.gene94468:CDS 35.0%
AAGTCACACGATGCTTAAAA+AGG + chr5.1:30299848-30299867 MS.gene94468:CDS 35.0%
AATGTTGTAGAGACTCAAGA+TGG + chr5.1:30298126-30298145 MS.gene94468:CDS 35.0%
AATTATTCAGCTCATGGTTC+GGG + chr5.1:30299186-30299205 MS.gene94468:CDS 35.0%
ACAACAATATTGCGAGTGTA+AGG + chr5.1:30299310-30299329 MS.gene94468:CDS 35.0%
ACACGATGCTTAAAAAGGAT+GGG + chr5.1:30299853-30299872 MS.gene94468:CDS 35.0%
AGGAGAAGTGAAACATTTCA+CGG - chr5.1:30299886-30299905 None:intergenic 35.0%
AGTGGAGATCTAACACTATT+TGG - chr5.1:30299663-30299682 None:intergenic 35.0%
ATCCTCTACAATCTTCTTCT+TGG - chr5.1:30299745-30299764 None:intergenic 35.0%
ATCTTGAGTCTCTACAACAT+TGG - chr5.1:30298127-30298146 None:intergenic 35.0%
CGTTGACAAGAAAATCAGAA+TGG + chr5.1:30297734-30297753 MS.gene94468:intron 35.0%
GTGTGTTTACTTCATGTTCA+TGG + chr5.1:30298756-30298775 MS.gene94468:intron 35.0%
TACCAAGAAGAAGATTGTAG+AGG + chr5.1:30299740-30299759 MS.gene94468:CDS 35.0%
TCTTGAGTCTCTACAACATT+GGG - chr5.1:30298126-30298145 None:intergenic 35.0%
TGAAACTTTGCAGGAAGATA+TGG + chr5.1:30299428-30299447 MS.gene94468:CDS 35.0%
TGAAGGTAATGACAACATTG+CGG + chr5.1:30299455-30299474 MS.gene94468:CDS 35.0%
TTATAAATCACGCCAACTTG+TGG - chr5.1:30299345-30299364 None:intergenic 35.0%
TTGAAGATTGAGTTAGAGAG+GGG - chr5.1:30297792-30297811 None:intergenic 35.0%
! AAATTTTCCTCCAAAAGGGA+AGG + chr5.1:30297582-30297601 MS.gene94468:CDS 35.0%
! AACCTCAATTATGGATTGTG+TGG - chr5.1:30298852-30298871 None:intergenic 35.0%
! AGCGAAATTTTCCTCCAAAA+GGG + chr5.1:30297578-30297597 MS.gene94468:CDS 35.0%
! ATCTAACACTATTTGGCTCA+TGG - chr5.1:30299656-30299675 None:intergenic 35.0%
! CAAGTTGGCGTGATTTATAA+TGG + chr5.1:30299345-30299364 MS.gene94468:CDS 35.0%
! CTGGTAATTTGTGTAGTAGT+AGG + chr5.1:30299799-30299818 MS.gene94468:CDS 35.0%
! TCTAACACTATTTGGCTCAT+GGG - chr5.1:30299655-30299674 None:intergenic 35.0%
!! AAATGGTTTTTCTTCTGAGG+TGG + chr5.1:30298197-30298216 MS.gene94468:intron 35.0%
!! TAGTGTCTGGAATCACTTTT+AGG + chr5.1:30298552-30298571 MS.gene94468:intron 35.0%
!!! AATGGTTTTTCTTCTGAGGT+GGG + chr5.1:30298198-30298217 MS.gene94468:intron 35.0%
!!! CTCATCGACTGTTTTTTTGT+GGG - chr5.1:30299268-30299287 None:intergenic 35.0%
!!! TCTCATCGACTGTTTTTTTG+TGG - chr5.1:30299269-30299288 None:intergenic 35.0%
!!! TTCATCAGCTGGTTTTTTGA+GGG - chr5.1:30299412-30299431 None:intergenic 35.0%
!!! TTTCATCAGCTGGTTTTTTG+AGG - chr5.1:30299413-30299432 None:intergenic 35.0%
AAACCTCCAAACCAATGCAA+GGG + chr5.1:30297941-30297960 MS.gene94468:intron 40.0%
AAACTATCACTTGGAGCTTC+AGG - chr5.1:30298808-30298827 None:intergenic 40.0%
AAATACCACGAGCCTAGATA+AGG - chr5.1:30298254-30298273 None:intergenic 40.0%
AATACCACGAGCCTAGATAA+GGG - chr5.1:30298253-30298272 None:intergenic 40.0%
AATACCCTTGTGTGTGATTG+TGG + chr5.1:30299071-30299090 MS.gene94468:intron 40.0%
ATTCCTCTTGACCCTTATCT+AGG + chr5.1:30298239-30298258 MS.gene94468:intron 40.0%
CACACGATGCTTAAAAAGGA+TGG + chr5.1:30299852-30299871 MS.gene94468:CDS 40.0%
CTCCACACAATCCATAATTG+AGG + chr5.1:30298847-30298866 MS.gene94468:intron 40.0%
GAAGGTAATGACAACATTGC+GGG + chr5.1:30299456-30299475 MS.gene94468:CDS 40.0%
GCAATGTTGTCATTACCTTC+AGG - chr5.1:30299456-30299475 None:intergenic 40.0%
GGGAAATGAATGGGAAAATG+TGG + chr5.1:30299206-30299225 MS.gene94468:CDS 40.0%
TGGTACGTTTCTTATCCTTG+CGG - chr5.1:30299725-30299744 None:intergenic 40.0%
TTAGAAATCACGTCAACCTG+CGG - chr5.1:30299555-30299574 None:intergenic 40.0%
! CAGACATGAGATTTAGAGTC+TGG + chr5.1:30298953-30298972 MS.gene94468:intron 40.0%
! CAGGTTGACGTGATTTCTAA+TGG + chr5.1:30299555-30299574 MS.gene94468:CDS 40.0%
! GAGCGAAATTTTCCTCCAAA+AGG + chr5.1:30297577-30297596 MS.gene94468:CDS 40.0%
! TCATGGTTCGGGAAATGAAT+GGG + chr5.1:30299197-30299216 MS.gene94468:CDS 40.0%
! TTACCTTCTACCTTCCCTTT+TGG - chr5.1:30297595-30297614 None:intergenic 40.0%
!! AAGCATCGTGTGACTTTTCT+TGG - chr5.1:30299844-30299863 None:intergenic 40.0%
!!! TTTTGATTTGTCTCAACCGC+AGG + chr5.1:30299536-30299555 MS.gene94468:CDS 40.0%
AACAGCCACAATCACACACA+AGG - chr5.1:30299079-30299098 None:intergenic 45.0%
AACAGTCGATGAGACTTCAC+AGG + chr5.1:30299275-30299294 MS.gene94468:CDS 45.0%
ACAGCCACAATCACACACAA+GGG - chr5.1:30299078-30299097 None:intergenic 45.0%
ACCAGCTGATGAAACTTTGC+AGG + chr5.1:30299419-30299438 MS.gene94468:CDS 45.0%
AGCCTGGTAGATCTTCTAAC+AGG - chr5.1:30299906-30299925 None:intergenic 45.0%
AGGTTGTCTCAACCACAAGT+TGG + chr5.1:30299330-30299349 MS.gene94468:CDS 45.0%
CAAGAGCTAAACCCTTGCAT+TGG - chr5.1:30297955-30297974 None:intergenic 45.0%
CAGGAAGATATGGAACCTGA+AGG + chr5.1:30299438-30299457 MS.gene94468:CDS 45.0%
CGAATTATCAAGTGACCGCA+AGG + chr5.1:30299707-30299726 MS.gene94468:CDS 45.0%
CGGTTGGATTTCCACACAAT+TGG + chr5.1:30299160-30299179 MS.gene94468:CDS 45.0%
CTCCTGTTAGAAGATCTACC+AGG + chr5.1:30299901-30299920 MS.gene94468:CDS 45.0%
GAAACCTCCAAACCAATGCA+AGG + chr5.1:30297940-30297959 MS.gene94468:intron 45.0%
GCAATGTTGTCATTGCCTTC+CGG - chr5.1:30299513-30299532 None:intergenic 45.0%
GTGGAAATCCAACCGAGAAA+CGG - chr5.1:30299155-30299174 None:intergenic 45.0%
GTTGTCTCAACCGGAAGATA+TGG + chr5.1:30299485-30299504 MS.gene94468:CDS 45.0%
TCAACCGGAAGATATGGAAC+CGG + chr5.1:30299491-30299510 MS.gene94468:CDS 45.0%
TCCTGCAAAGTTTCATCAGC+TGG - chr5.1:30299423-30299442 None:intergenic 45.0%
TCTTGCTGGCATTGCTTCAA+TGG + chr5.1:30297970-30297989 MS.gene94468:intron 45.0%
! ACACTATTTGGCTCATGGGT+AGG - chr5.1:30299651-30299670 None:intergenic 45.0%
! CTCATGGTTCGGGAAATGAA+TGG + chr5.1:30299196-30299215 MS.gene94468:CDS 45.0%
! GGGTCTTAAGTTGTCTCAAC+CGG + chr5.1:30299476-30299495 MS.gene94468:CDS 45.0%
! GTCTGGCAATTTTGTCTGAC+AGG + chr5.1:30298970-30298989 MS.gene94468:intron 45.0%
! TTGTGACTATTGGTGCTTGC+AGG + chr5.1:30298055-30298074 MS.gene94468:intron 45.0%
!! AAACCCTTGCATTGGTTTGG+AGG - chr5.1:30297947-30297966 None:intergenic 45.0%
!! GCTAAACCCTTGCATTGGTT+TGG - chr5.1:30297950-30297969 None:intergenic 45.0%
!!! ACCATTTTGAGACGTACGTG+AGG - chr5.1:30298184-30298203 None:intergenic 45.0%
!!! AATTTATTTAAAATCAATTT+TGG - chr5.1:30298344-30298363 None:intergenic 5.0%
ACAGGTATGTCGCCGTTTCT+CGG + chr5.1:30299140-30299159 MS.gene94468:intron 50.0%
CCTCCAAAAGGGAAGGTAGA+AGG + chr5.1:30297589-30297608 MS.gene94468:intron 50.0%
CCTTCCGGTTCCATATCTTC+CGG - chr5.1:30299498-30299517 None:intergenic 50.0%
GAGACTTCACAGGAAGCTGA+AGG + chr5.1:30299285-30299304 MS.gene94468:CDS 50.0%
GAGCCTAGATAAGGGTCAAG+AGG - chr5.1:30298245-30298264 None:intergenic 50.0%
GATTGAGTTAGAGAGGGGTG+TGG - chr5.1:30297787-30297806 None:intergenic 50.0%
GCCTCACGTACGTCTCAAAA+TGG + chr5.1:30298180-30298199 MS.gene94468:CDS 50.0%
GGCATCACTGTAATCTTCCG+CGG + chr5.1:30298147-30298166 MS.gene94468:CDS 50.0%
GTTCATGGGTCATCCACAGT+GGG + chr5.1:30298771-30298790 MS.gene94468:intron 50.0%
TGGCTTCGAAGACCCCAATA+CGG + chr5.1:30297754-30297773 MS.gene94468:intron 50.0%
TGTTCATGGGTCATCCACAG+TGG + chr5.1:30298770-30298789 MS.gene94468:intron 50.0%
TTGACCCTTATCTAGGCTCG+TGG + chr5.1:30298246-30298265 MS.gene94468:intron 50.0%
! CCTTCTACCTTCCCTTTTGG+AGG - chr5.1:30297592-30297611 None:intergenic 50.0%
! GGTTCAAGTTCAACCCACTG+TGG - chr5.1:30298787-30298806 None:intergenic 50.0%
! GTGAGGCGTTAATCAAACCG+CGG - chr5.1:30298167-30298186 None:intergenic 50.0%
!! TGCAAGGGTTTAGCTCTTGC+TGG + chr5.1:30297956-30297975 MS.gene94468:intron 50.0%
CCGGAAGATATGGAACCGGA+AGG + chr5.1:30299495-30299514 MS.gene94468:CDS 55.0%
GTATGTCGCCGTTTCTCGGT+TGG + chr5.1:30299144-30299163 MS.gene94468:CDS 55.0%
! TGAGTTAGAGAGGGGTGTGG+TGG - chr5.1:30297784-30297803 None:intergenic 55.0%
!! GTGTGGTGGTGGACCGTATT+GGG - chr5.1:30297770-30297789 None:intergenic 55.0%
!! TGTGGTGGTGGACCGTATTG+GGG - chr5.1:30297769-30297788 None:intergenic 55.0%
! GTTAGAGAGGGGTGTGGTGG+TGG - chr5.1:30297781-30297800 None:intergenic 60.0%
!! GGTGTGGTGGTGGACCGTAT+TGG - chr5.1:30297771-30297790 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 30297569 30299944 30297569 ID=MS.gene94468
chr5.1 mRNA 30297569 30299944 30297569 ID=MS.gene94468.t1;Parent=MS.gene94468
chr5.1 exon 30297569 30297603 30297569 ID=MS.gene94468.t1.exon1;Parent=MS.gene94468.t1
chr5.1 CDS 30297569 30297603 30297569 ID=cds.MS.gene94468.t1;Parent=MS.gene94468.t1
chr5.1 exon 30298077 30298215 30298077 ID=MS.gene94468.t1.exon2;Parent=MS.gene94468.t1
chr5.1 CDS 30298077 30298215 30298077 ID=cds.MS.gene94468.t1;Parent=MS.gene94468.t1
chr5.1 exon 30299144 30299944 30299144 ID=MS.gene94468.t1.exon3;Parent=MS.gene94468.t1
chr5.1 CDS 30299144 30299944 30299144 ID=cds.MS.gene94468.t1;Parent=MS.gene94468.t1
Gene Sequence

>MS.gene94468

ATGTTTAGGAGCGAAATTTTCCTCCAAAAGGGAAGGTAGAAGGTAAAGAGAAGCTTAGCAAAAACAAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAAAGAAAACTGCTCGTTGACAAGAAAATCAGAATGGCTTCGAAGACCCCAATACGGTCCACCACCACACCCCTCTCTAACTCAATCTTCAAATCTAAACATGTATTTCCCATTCACTCTTCTCTCTTGTTTCTTTTCTTCATGAAATTATTCACAATATACACACTCATGAATTTACTTGCTTGTTTTTGTAGATTTTCTTACATGAATGGTGGCTTGTGAAACCTCCAAACCAATGCAAGGGTTTAGCTCTTGCTGGCATTGCTTCAATGGAGTAATTTTTTTCATTTTCATTTTTAGGTTTCTAATTGGTTATCTGTTTGGGTAATTACTTATTGTGACTATTGGTGCTTGCAGGAGAGAAAGAATGTTTTTTTCTTCAGTGATTGTAGAGAGACATGAACCCAATGTTGTAGAGACTCAAGATGGCATCACTGTAATCTTCCGCGGTTTGATTAACGCCTCACGTACGTCTCAAAATGGTTTTTCTTCTGAGGTGGGTTACCCTTTTTCTTTTTCATTCCTCTTGACCCTTATCTAGGCTCGTGGTATTTTACTAGCTGCTAATAGAGTTGATTTTGAATATAATTAGTGTGTTTGGTTATGCTGTGACTTCTGTGACCAAAATTGATTTTAAATAAATTCATTTTTTATTGAAATTGAGTTGTATGTAAAACGTTGTGTTTTGATACATTCGAGTAAAACTGAATTGAATAATAATTTGAGAGTACAAATCAAATTTGGATGCATGAGCTAATTTCAGCTTCAAGTTAGAATTCATTTTTGTGGCAAAGAGTGATTCGACATAAGAATATTTTAAACATGTCAACATTAGTGTCTGGAATCACTTTTAGGCCTCTCTTAAAAGTGAATCTAAACATACACTTAAATTTTAGAATTAGTTTTGGTTTAATTGAGTTTGAAGTTAATTTCATTTATGTTCACATGTTTTTACTTCCAAAGAAAGTTTGATACAAAAAGTAGTGTTAAATTTTTAATTCTTCACAAAATTTGCTGTTTATCAATACCTTTTTGTGTGTGTTTACTTCATGTTCATGGGTCATCCACAGTGGGTTGAACTTGAACCTGAAGCTCCAAGTGATAGTTTCAAAGTTCAAACACTCTGACTCCACACAATCCATAATTGAGGTTTATCAATCATTCAGTTTTATTTGGTAGAAATCAAACTCCTATATTTTTGCTAGCTTTGTTAGGTGAAACTAGACACACGCACAGACATGAGATTTAGAGTCTGGCAATTTTGTCTGACAGGAACCCAAAATCTATACTTTTTGACAAAATCAATTCTGTTAGTGAAACAAAATACTCATAATCTTCAATGGATTGAATAAATACCCTTGTGTGTGATTGTGGCTGTTATTGACACATTTAATTTTCTGGAAATATTCAATGTCTTTCTACAGGTATGTCGCCGTTTCTCGGTTGGATTTCCACACAATTGGAAAAATTATTCAGCTCATGGTTCGGGAAATGAATGGGAAAATGTGGATAAAGTTACTGGTTTTGACGACTCAAATACTTCTTCCCACAAAAAAACAGTCGATGAGACTTCACAGGAAGCTGAAGGTAACAACAATATTGCGAGTGTAAGGTTGTCTCAACCACAAGTTGGCGTGATTTATAATGGTGAAAATGGATTCTCAAATGTTGACAACTCAAATGTTTCATCCCTCAAAAAACCAGCTGATGAAACTTTGCAGGAAGATATGGAACCTGAAGGTAATGACAACATTGCGGGTCTTAAGTTGTCTCAACCGGAAGATATGGAACCGGAAGGCAATGACAACATTGCGAGTTTTGATTTGTCTCAACCGCAGGTTGACGTGATTTCTAATGGTGAAAATGGAGTCTCAAGTGTTGCTGCAGCTGACAATAGTCAATCTTTGATGAGTGTATTTGAATTTGATCCTACCCATGAGCCAAATAGTGTTAGATCTCCACTTTACGCAAAGAAATTGAAGTTTGACGAATTATCAAGTGACCGCAAGGATAAGAAACGTACCAAGAAGAAGATTGTAGAGGATAAGAAACGTACCAAGAAGAAGATTGTAGAGGATACTGGTAATTTGTGTAGTAGTAGGGTGCTGACAAGAAGCATTGCCAAGAAAAGTCACACGATGCTTAAAAAGGATGGGAAAACTGCCGTGAAATGTTTCACTTCTCCTGTTAGAAGATCTACCAGGCTACTAAATTATAAAAAATGA
Protein sequence

>MS.gene94468.t1

MFRSEIFLQKGRRERMFFSSVIVERHEPNVVETQDGITVIFRGLINASRTSQNGFSSEVCRRFSVGFPHNWKNYSAHGSGNEWENVDKVTGFDDSNTSSHKKTVDETSQEAEGNNNIASVRLSQPQVGVIYNGENGFSNVDNSNVSSLKKPADETLQEDMEPEGNDNIAGLKLSQPEDMEPEGNDNIASFDLSQPQVDVISNGENGVSSVAAADNSQSLMSVFEFDPTHEPNSVRSPLYAKKLKFDELSSDRKDKKRTKKKIVEDKKRTKKKIVEDTGNLCSSRVLTRSIAKKSHTMLKKDGKTAVKCFTSPVRRSTRLLNYKK