AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94531

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94531.t1 MTR_8g100085 63.942 208 15 2 5 152 180 387 1.64e-83 251
MS.gene94531.t1 MTR_3g034100 62.981 208 17 2 5 152 129 336 2.14e-82 247
MS.gene94531.t1 MTR_4g132860 38.350 206 67 2 7 152 129 334 6.47e-40 138
MS.gene94531.t1 MTR_4g088750 35.922 206 72 2 7 152 251 456 1.14e-39 139
MS.gene94531.t1 MTR_4g105770 36.408 206 71 2 7 152 107 312 4.05e-38 133
MS.gene94531.t1 MTR_4g088755 35.437 206 73 2 7 152 90 295 1.01e-37 132
MS.gene94531.t1 MTR_4g088745 35.437 206 73 2 7 152 95 300 1.07e-37 132
MS.gene94531.t1 MTR_3g078000 36.893 206 70 2 7 152 106 311 3.23e-37 130
MS.gene94531.t1 MTR_5g023150 45.736 129 61 1 33 152 319 447 4.73e-36 129
MS.gene94531.t1 MTR_1g026160 41.975 162 74 3 1 152 243 394 5.08e-36 129
MS.gene94531.t1 MTR_5g023150 45.736 129 61 1 33 152 416 544 1.14e-35 129
MS.gene94531.t1 MTR_3g114900 35.122 205 74 2 7 152 315 519 1.19e-34 126
MS.gene94531.t1 MTR_2g013900 33.495 206 77 2 7 152 212 417 1.89e-34 125
MS.gene94531.t1 MTR_6g004440 40.782 179 69 7 7 152 74 248 3.68e-33 119
MS.gene94531.t1 MTR_7g072710 35.981 214 69 5 7 152 93 306 8.15e-33 119
MS.gene94531.t1 MTR_7g072710 35.981 214 69 5 7 152 56 269 8.93e-33 118
MS.gene94531.t1 MTR_1g113960 43.411 129 64 1 33 152 422 550 2.21e-32 120
MS.gene94531.t1 MTR_1g113960 43.411 129 64 1 33 152 422 550 2.47e-32 120
MS.gene94531.t1 MTR_1g099110 47.200 125 56 2 33 148 387 510 3.02e-31 117
MS.gene94531.t1 MTR_1g099110 47.200 125 56 2 33 148 404 527 4.17e-31 117
MS.gene94531.t1 MTR_3g078110 41.538 130 66 2 33 152 205 334 6.48e-29 108
MS.gene94531.t1 MTR_1g103270 43.077 130 64 2 33 152 224 353 9.19e-29 108
MS.gene94531.t1 MTR_3g076970 34.884 215 69 6 7 152 74 286 2.02e-28 107
MS.gene94531.t1 MTR_6g007603 40.769 130 67 2 33 152 217 346 1.32e-27 105
MS.gene94531.t1 MTR_5g006560 40.000 130 68 2 33 152 232 361 5.71e-27 104
MS.gene94531.t1 MTR_5g006560 40.000 130 68 2 33 152 266 395 8.80e-27 103
MS.gene94531.t1 MTR_4g106980 39.231 130 69 2 33 152 234 363 9.66e-27 103
MS.gene94531.t1 MTR_5g066490 38.312 154 77 5 9 149 749 897 8.49e-25 99.4
MS.gene94531.t1 MTR_1g026160 42.400 125 52 3 1 115 388 502 2.17e-23 95.1
MS.gene94531.t1 MTR_1g026160 42.400 125 52 3 1 115 243 357 2.75e-23 94.0
MS.gene94531.t1 MTR_1g026160 42.400 125 52 3 1 115 241 355 2.82e-23 94.0
MS.gene94531.t1 MTR_1g026160 43.089 123 50 3 1 113 243 355 4.87e-23 93.2
MS.gene94531.t1 MTR_1g026160 43.089 123 50 3 1 113 241 353 5.44e-23 92.8
MS.gene94531.t1 MTR_1g026160 43.089 123 50 3 1 113 388 500 6.45e-23 94.0
MS.gene94531.t1 MTR_3g100090 36.538 156 83 4 9 152 756 907 1.53e-22 93.2
MS.gene94531.t1 MTR_1g026160 45.631 103 45 2 1 102 241 333 4.24e-22 90.5
MS.gene94531.t1 MTR_2g034020 35.862 145 80 4 20 152 575 718 4.99e-21 88.6
MS.gene94531.t1 MTR_6g079540 36.129 155 85 4 9 152 513 664 5.60e-21 88.6
MS.gene94531.t1 MTR_6g079540 36.129 155 85 4 9 152 761 912 5.83e-21 88.6
MS.gene94531.t1 MTR_1g081790 34.932 146 75 3 17 152 806 941 2.77e-20 86.7
MS.gene94531.t1 MTR_1g016300 33.784 148 76 4 16 152 779 915 1.14e-19 84.7
MS.gene94531.t1 MTR_2g461180 35.762 151 70 6 14 148 1247 1386 1.42e-19 84.7
MS.gene94531.t1 MTR_4g122160 37.903 124 65 3 33 145 583 705 3.61e-19 83.2
MS.gene94531.t1 MTR_8g107000 34.615 130 75 2 33 152 852 981 1.76e-18 81.3
MS.gene94531.t1 MTR_4g116520 35.099 151 71 6 14 148 1212 1351 3.80e-18 80.5
MS.gene94531.t1 MTR_6g033130 34.194 155 75 6 14 152 1087 1230 3.84e-18 80.5
MS.gene94531.t1 MTR_8g442400 34.194 155 75 5 14 152 1506 1649 4.71e-18 80.1
MS.gene94531.t1 MTR_3g465590 32.090 134 77 3 33 152 447 580 1.42e-17 78.6
MS.gene94531.t1 MTR_4g082325 26.761 213 89 6 7 152 189 401 1.62e-17 78.2
MS.gene94531.t1 MTR_3g104900 30.864 162 86 4 7 152 287 438 3.61e-17 77.4
MS.gene94531.t1 MTR_1g090020 31.973 147 73 6 18 148 1024 1159 4.53e-17 77.4
MS.gene94531.t1 MTR_5g072500 34.615 104 55 2 62 152 480 583 2.25e-16 75.5
MS.gene94531.t1 MTR_1g050690 33.333 147 81 5 20 152 528 671 2.61e-16 75.1
MS.gene94531.t1 MTR_1g061190 32.215 149 79 4 15 152 672 809 3.07e-16 75.1
MS.gene94531.t1 MTR_5g010730 30.822 146 79 4 15 149 538 672 7.29e-16 73.9
MS.gene94531.t1 MTR_5g010730 30.822 146 79 4 15 149 593 727 7.37e-16 73.9
MS.gene94531.t1 MTR_4g106980 50.746 67 32 1 33 98 234 300 9.90e-16 72.8
MS.gene94531.t1 MTR_5g019700 28.440 218 80 9 5 152 90 301 1.11e-15 73.2
MS.gene94531.t1 MTR_4g128650 32.000 150 77 5 14 148 931 1070 3.63e-15 72.0
MS.gene94531.t1 MTR_1g081790 34.884 129 64 3 17 135 806 924 7.52e-15 70.9
MS.gene94531.t1 MTR_4g080030 23.558 208 93 7 7 152 592 795 2.75e-14 69.3
MS.gene94531.t1 MTR_5g055070 44.595 74 38 2 33 103 624 697 4.34e-12 63.2
MS.gene94531.t1 MTR_5g055070 44.595 74 38 2 33 103 582 655 5.79e-12 62.8
MS.gene94531.t1 MTR_5g055070 44.595 74 38 2 33 103 624 697 6.87e-12 62.4
MS.gene94531.t1 MTR_5g005530 42.308 78 42 1 33 107 453 530 7.75e-12 62.4
MS.gene94531.t1 MTR_5g068190 41.892 74 40 2 33 103 464 537 7.86e-12 62.4
MS.gene94531.t1 MTR_5g065130 45.946 74 37 2 33 103 465 538 1.78e-11 61.2
MS.gene94531.t1 MTR_1g105650 40.260 77 43 2 33 106 479 555 2.31e-11 60.8
MS.gene94531.t1 MTR_6g068970 28.758 153 83 5 26 152 845 997 3.35e-11 60.5
MS.gene94531.t1 MTR_1g050520 26.994 163 76 3 33 152 150 312 3.59e-11 60.5
MS.gene94531.t1 MTR_1g105710 44.118 68 35 2 33 97 476 543 3.61e-11 60.5
MS.gene94531.t1 MTR_5g059940 28.000 150 85 5 11 149 236 373 9.32e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94531.t1 AT4G31170 56.250 208 31 2 5 152 178 385 2.14e-68 213
MS.gene94531.t1 AT4G31170 56.250 208 31 2 5 152 178 385 2.14e-68 213
MS.gene94531.t1 AT4G31170 56.250 208 31 2 5 152 178 385 2.14e-68 213
MS.gene94531.t1 AT4G31170 56.250 208 31 2 5 152 178 385 2.14e-68 213
MS.gene94531.t1 AT4G31170 56.250 208 31 2 5 152 178 385 2.14e-68 213
MS.gene94531.t1 AT4G31170 56.250 208 31 2 5 152 178 385 2.14e-68 213
MS.gene94531.t1 AT4G31170 56.250 208 31 2 5 152 178 385 2.14e-68 213
MS.gene94531.t1 AT2G24360 55.610 205 31 2 8 152 180 384 2.30e-68 212
MS.gene94531.t1 AT1G62400 39.320 206 65 2 7 152 135 340 3.53e-41 141
MS.gene94531.t1 AT4G35780 45.736 129 61 1 33 152 413 541 1.32e-35 129
MS.gene94531.t1 AT2G17700 40.123 162 77 3 1 152 384 535 1.34e-34 126
MS.gene94531.t1 AT4G38470 34.146 205 76 3 7 152 335 539 1.27e-33 124
MS.gene94531.t1 AT4G38470 34.146 205 76 3 7 152 335 539 1.53e-33 124
MS.gene94531.t1 AT5G58950 32.524 206 79 2 7 152 258 463 7.99e-33 121
MS.gene94531.t1 AT5G01850 34.579 214 72 4 7 152 66 279 4.52e-32 116
MS.gene94531.t1 AT5G01850 45.652 138 57 3 33 152 166 303 1.77e-30 113
MS.gene94531.t1 AT3G27560 34.562 217 67 6 7 152 74 286 2.22e-30 112
MS.gene94531.t1 AT3G63260 41.538 130 66 2 33 152 233 362 2.73e-30 113
MS.gene94531.t1 AT5G50180 34.419 215 70 6 7 152 68 280 4.97e-30 111
MS.gene94531.t1 AT5G50000 41.538 130 66 2 33 152 227 356 7.81e-29 108
MS.gene94531.t1 AT5G40540 34.562 217 67 6 7 152 74 286 8.38e-29 108
MS.gene94531.t1 AT3G46930 40.000 130 68 1 33 152 291 420 1.03e-28 109
MS.gene94531.t1 AT3G01490 40.769 130 67 2 33 152 253 382 1.39e-28 108
MS.gene94531.t1 AT3G46930 40.000 130 68 1 33 152 326 455 1.56e-28 109
MS.gene94531.t1 AT5G40540 34.112 214 66 6 10 152 1 210 3.74e-28 105
MS.gene94531.t1 AT3G22750 39.231 130 69 2 33 152 220 349 5.54e-28 106
MS.gene94531.t1 AT4G31170 48.062 129 16 1 3 80 176 304 1.18e-27 104
MS.gene94531.t1 AT4G31170 48.062 129 16 1 3 80 176 304 1.18e-27 104
MS.gene94531.t1 AT4G31170 48.062 129 16 1 3 80 176 304 1.18e-27 104
MS.gene94531.t1 AT5G40540 44.681 141 53 5 33 152 53 189 1.83e-27 103
MS.gene94531.t1 AT4G14780 41.538 130 66 2 33 152 206 335 5.22e-27 103
MS.gene94531.t1 AT4G38470 34.940 166 49 3 7 113 335 500 5.92e-24 97.1
MS.gene94531.t1 AT5G66710 33.182 220 72 9 3 152 116 330 6.55e-24 96.3
MS.gene94531.t1 AT4G24480 37.821 156 81 4 9 152 772 923 8.17e-23 94.0
MS.gene94531.t1 AT4G24480 37.821 156 81 4 9 152 772 923 8.18e-23 94.0
MS.gene94531.t1 AT4G24480 37.821 156 81 4 9 152 777 928 8.18e-23 94.0
MS.gene94531.t1 AT3G50730 30.804 224 69 8 5 152 83 296 1.12e-22 92.4
MS.gene94531.t1 AT1G08720 35.366 164 83 4 2 152 768 921 2.79e-22 92.4
MS.gene94531.t1 AT1G67890 35.664 143 82 2 20 152 596 738 2.83e-21 89.4
MS.gene94531.t1 AT5G11850 34.694 147 76 3 16 152 725 861 1.58e-20 87.4
MS.gene94531.t1 AT5G49470 35.862 145 80 4 20 152 313 456 2.03e-20 86.7
MS.gene94531.t1 AT5G49470 35.862 145 80 4 20 152 473 616 2.33e-20 86.7
MS.gene94531.t1 AT5G49470 35.862 145 80 4 20 152 574 717 2.38e-20 86.7
MS.gene94531.t1 AT5G49470 35.862 145 80 4 20 152 600 743 2.40e-20 86.7
MS.gene94531.t1 AT5G49470 35.862 145 80 4 20 152 661 804 2.42e-20 86.7
MS.gene94531.t1 AT1G14000 28.169 213 86 6 7 152 208 420 6.18e-20 85.1
MS.gene94531.t1 AT1G73660 33.103 145 84 4 20 152 857 1000 3.47e-19 83.6
MS.gene94531.t1 AT5G40540 32.571 175 50 4 7 113 74 248 3.52e-19 81.6
MS.gene94531.t1 AT2G35050 35.762 151 70 6 14 148 1093 1232 4.95e-19 83.2
MS.gene94531.t1 AT2G35050 35.762 151 70 6 14 148 1093 1232 4.95e-19 83.2
MS.gene94531.t1 AT1G18160 35.385 130 74 2 33 152 838 967 5.23e-19 83.2
MS.gene94531.t1 AT1G04700 34.839 155 74 6 14 152 886 1029 7.37e-19 82.4
MS.gene94531.t1 AT1G04700 34.839 155 74 6 14 152 886 1029 7.37e-19 82.4
MS.gene94531.t1 AT1G16270 35.484 155 73 6 14 152 983 1126 2.05e-18 81.3
MS.gene94531.t1 AT1G16270 35.484 155 73 6 14 152 983 1126 2.05e-18 81.3
MS.gene94531.t1 AT1G16270 35.484 155 73 6 14 152 983 1126 2.05e-18 81.3
MS.gene94531.t1 AT3G06630 33.793 145 83 4 20 152 543 686 2.96e-18 80.9
MS.gene94531.t1 AT3G24715 32.903 155 77 6 14 152 956 1099 3.73e-18 80.5
MS.gene94531.t1 AT3G24715 32.903 155 77 6 14 152 956 1099 3.73e-18 80.5
MS.gene94531.t1 AT3G24715 32.903 155 77 6 14 152 956 1099 3.73e-18 80.5
MS.gene94531.t1 AT3G63260 53.731 67 30 1 33 98 233 299 5.25e-18 79.3
MS.gene94531.t1 AT3G50720 28.767 219 79 8 5 152 95 307 5.51e-18 79.3
MS.gene94531.t1 AT3G06620 33.103 145 84 4 20 152 603 746 9.22e-18 79.3
MS.gene94531.t1 AT1G79570 34.839 155 74 6 14 152 1084 1227 1.14e-17 79.3
MS.gene94531.t1 AT1G79570 34.839 155 74 6 14 152 1084 1227 1.14e-17 79.3
MS.gene94531.t1 AT1G67890 37.302 126 69 2 20 135 468 593 1.44e-17 79.0
MS.gene94531.t1 AT1G67890 37.302 126 69 2 20 135 596 721 1.49e-17 79.0
MS.gene94531.t1 AT1G67890 37.302 126 69 2 20 135 596 721 1.49e-17 79.0
MS.gene94531.t1 AT3G06640 32.414 145 85 4 20 152 555 698 1.03e-16 76.3
MS.gene94531.t1 AT3G06640 32.414 145 85 4 20 152 555 698 1.03e-16 76.3
MS.gene94531.t1 AT3G06640 32.414 145 85 4 20 152 555 698 1.14e-16 76.3
MS.gene94531.t1 AT4G23050 31.544 149 80 4 15 152 582 719 1.18e-16 76.3
MS.gene94531.t1 AT4G23050 31.544 149 80 4 15 152 583 720 1.18e-16 76.3
MS.gene94531.t1 AT5G03730 34.228 149 76 4 15 152 668 805 1.46e-16 75.9
MS.gene94531.t1 AT5G03730 34.228 149 76 4 15 152 668 805 1.46e-16 75.9
MS.gene94531.t1 AT1G08720 34.899 149 74 4 2 137 768 906 1.49e-16 75.9
MS.gene94531.t1 AT5G57610 33.113 151 74 6 14 148 901 1040 2.43e-16 75.5
MS.gene94531.t1 AT2G31010 26.699 206 83 7 7 149 564 764 4.70e-16 74.3
MS.gene94531.t1 AT2G31010 26.699 206 83 7 7 149 564 764 4.70e-16 74.3
MS.gene94531.t1 AT3G46920 33.803 142 77 5 27 152 929 1069 5.86e-16 74.3
MS.gene94531.t1 AT3G46920 33.803 142 77 5 27 152 995 1135 5.89e-16 74.3
MS.gene94531.t1 AT3G58640 24.519 208 91 6 7 152 598 801 1.14e-15 73.6
MS.gene94531.t1 AT3G58640 24.519 208 91 6 7 152 598 801 1.14e-15 73.6
MS.gene94531.t1 AT5G50000 50.746 67 32 1 33 98 227 293 1.16e-15 72.8
MS.gene94531.t1 AT3G01490 50.746 67 32 1 33 98 253 319 1.81e-15 72.4
MS.gene94531.t1 AT3G06630 35.938 128 69 4 20 135 543 669 3.14e-15 72.0
MS.gene94531.t1 AT5G49470 43.023 86 45 3 20 102 600 684 8.29e-15 70.9
MS.gene94531.t1 AT5G49470 43.023 86 45 3 20 102 600 684 8.29e-15 70.9
MS.gene94531.t1 AT1G18160 35.652 115 64 2 33 137 838 952 1.42e-14 70.1
MS.gene94531.t1 AT3G46930 39.796 98 49 1 26 113 223 320 4.38e-14 68.6
MS.gene94531.t1 AT5G07140 30.597 134 79 3 33 152 444 577 4.43e-14 68.9
MS.gene94531.t1 AT3G06630 43.023 86 45 3 20 102 459 543 5.49e-14 68.6
MS.gene94531.t1 AT3G06630 43.023 86 45 3 20 102 543 627 5.63e-14 68.6
MS.gene94531.t1 AT3G06630 43.023 86 45 3 20 102 543 627 5.63e-14 68.6
MS.gene94531.t1 AT3G06630 43.023 86 45 3 20 102 543 627 5.63e-14 68.6
MS.gene94531.t1 AT3G46930 39.796 98 49 1 26 113 284 381 5.80e-14 68.2
MS.gene94531.t1 AT3G46930 39.796 98 49 1 26 113 319 416 5.80e-14 68.2
MS.gene94531.t1 AT3G46930 39.796 98 49 1 26 113 319 416 5.82e-14 68.2
MS.gene94531.t1 AT5G58520 34.000 100 53 2 62 148 495 594 3.61e-13 66.2
MS.gene94531.t1 AT2G42640 26.570 207 85 8 7 152 535 735 4.57e-13 65.9
MS.gene94531.t1 AT2G42640 26.570 207 85 8 7 152 573 773 4.60e-13 65.9
MS.gene94531.t1 AT5G62710 30.000 170 81 6 11 152 389 548 2.68e-12 63.5
MS.gene94531.t1 AT5G62710 30.000 170 81 6 11 152 423 582 2.71e-12 63.5
MS.gene94531.t1 AT4G04510 44.595 74 38 2 33 103 464 537 5.22e-12 62.8
MS.gene94531.t1 AT4G04510 44.595 74 38 2 33 103 466 539 5.22e-12 62.8
MS.gene94531.t1 AT4G23270 43.421 76 40 2 33 105 451 526 9.56e-12 62.0
MS.gene94531.t1 AT4G23270 43.421 76 40 2 33 105 451 526 9.56e-12 62.0
MS.gene94531.t1 AT4G23270 43.421 76 40 2 33 105 451 526 9.57e-12 62.0
MS.gene94531.t1 AT1G72180 41.975 81 46 1 26 105 806 886 1.19e-11 62.0
MS.gene94531.t1 AT4G23180 45.946 74 37 2 33 103 473 546 1.27e-11 61.6
MS.gene94531.t1 AT4G23180 45.946 74 37 2 33 103 473 546 1.30e-11 61.6
MS.gene94531.t1 AT3G04810 23.871 155 101 3 4 149 107 253 1.64e-11 61.2
MS.gene94531.t1 AT3G04810 23.871 155 101 3 4 149 107 253 1.64e-11 61.2
MS.gene94531.t1 AT3G04810 23.871 155 101 3 4 149 107 253 1.64e-11 61.2
MS.gene94531.t1 AT4G23190 43.243 74 39 2 33 103 475 548 1.66e-11 61.2
MS.gene94531.t1 AT4G23190 43.243 74 39 2 33 103 397 470 1.66e-11 61.2
MS.gene94531.t1 AT4G23190 43.243 74 39 2 33 103 397 470 1.66e-11 61.2
MS.gene94531.t1 AT4G23190 43.243 74 39 2 33 103 475 548 1.70e-11 61.2
MS.gene94531.t1 AT2G35620 28.824 170 83 7 11 152 412 571 2.28e-11 60.8
MS.gene94531.t1 AT2G35620 28.824 170 83 7 11 152 412 571 2.28e-11 60.8
MS.gene94531.t1 AT2G35620 28.824 170 83 7 11 152 412 571 2.28e-11 60.8
MS.gene94531.t1 AT2G35620 28.824 170 83 7 11 152 412 571 2.28e-11 60.8
MS.gene94531.t1 AT1G70450 36.559 93 47 2 13 103 164 246 2.72e-11 60.5
MS.gene94531.t1 AT4G05200 45.946 74 37 2 33 103 472 545 2.91e-11 60.8
MS.gene94531.t1 AT4G05200 45.946 74 37 2 33 103 490 563 2.92e-11 60.8
MS.gene94531.t1 AT5G49660 30.636 173 77 8 11 152 773 933 3.91e-11 60.5
MS.gene94531.t1 AT4G04490 41.892 74 40 2 33 103 465 538 4.39e-11 60.1
MS.gene94531.t1 AT4G23280 40.789 76 42 2 33 105 419 494 6.82e-11 59.7
MS.gene94531.t1 AT4G23280 40.789 76 42 2 33 105 467 542 6.90e-11 59.7
MS.gene94531.t1 AT4G23280 40.789 76 42 2 33 105 469 544 6.91e-11 59.7

Find 37 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTTTATGATTGTTCTC+TGG 0.287119 3.2:+15543905 MS.gene94531:CDS
GTTCTCTGGGTGCTCATAAC+TGG 0.370868 3.2:+15543919 MS.gene94531:CDS
TAGCTTTATGATTGTTCTCT+GGG 0.377172 3.2:+15543906 MS.gene94531:CDS
TCAATCAAAATTGCTGACTT+TGG 0.379506 3.2:+15543769 MS.gene94531:CDS
GTCATTATTTCACGGAGAAC+AGG 0.380682 3.2:-15544826 None:intergenic
GCAAATGGTGGTTTAACATC+AGG 0.402931 3.2:-15544868 None:intergenic
CCCAACATAGTTCATTTCAT+AGG 0.415315 3.2:+15543554 MS.gene94531:CDS
GAGAACAGGCAAACAATCAT+TGG 0.416206 3.2:-15544812 None:intergenic
ATTGCTATAATTTGAGCAAA+TGG 0.417856 3.2:-15544883 None:intergenic
AAACAATCATTGGGTAAGAT+TGG 0.436034 3.2:-15544802 None:intergenic
CTTTGGAGTCGCTCGGATTG+AGG 0.447019 3.2:+15543786 MS.gene94531:CDS
GTGAAATAATGACAAGGTGC+TGG 0.454947 3.2:+15544836 MS.gene94531:CDS
ACCTATGAAATGAACTATGT+TGG 0.463147 3.2:-15543555 None:intergenic
ACATCTACTTTAAGTGTGTA+AGG 0.463881 3.2:-15543880 None:intergenic
GAAAATGATAAAATGTCAGC+TGG 0.487116 3.2:+15543502 None:intergenic
AGCACCTTGTCATTATTTCA+CGG 0.488236 3.2:-15544834 None:intergenic
AGGTGGATGCCGCAAGCCTA+TGG 0.488338 3.2:+15543574 MS.gene94531:CDS
AACATACTGTTGGATGGCTC+CGG 0.501805 3.2:+15543840 MS.gene94531:CDS
TAAGTGTGTAAGGCCTATGT+TGG 0.512303 3.2:-15543870 None:intergenic
CCTATGAAATGAACTATGTT+GGG 0.515033 3.2:-15543554 None:intergenic
GGCCTATGTTGGATCATCTC+CGG 0.516788 3.2:-15543859 None:intergenic
GAAGGAATGACTCTCAAGAC+TGG 0.526345 3.2:+15543817 MS.gene94531:CDS
TGAAATAATGACAAGGTGCT+GGG 0.534114 3.2:+15544837 MS.gene94531:CDS
AACATAGTTCATTTCATAGG+TGG 0.534861 3.2:+15543557 MS.gene94531:CDS
CAAATGGTGGTTTAACATCA+GGG 0.536280 3.2:-15544867 None:intergenic
AGAACAGGCAAACAATCATT+GGG 0.539053 3.2:-15544811 None:intergenic
TCAAGACTGGAACATACTGT+TGG 0.539569 3.2:+15543830 MS.gene94531:CDS
CGGATTGAGGTGCAAACTGA+AGG 0.553017 3.2:+15543799 MS.gene94531:CDS
GATGCCGCAAGCCTATGGTC+TGG 0.578790 3.2:+15543579 MS.gene94531:CDS
GACTGGAACATACTGTTGGA+TGG 0.580572 3.2:+15543834 MS.gene94531:CDS
GCTATAATTTGAGCAAATGG+TGG 0.591898 3.2:-15544880 None:intergenic
TTGCTGACTTTGGAGTCGCT+CGG 0.603139 3.2:+15543779 MS.gene94531:CDS
GTAACGATGTACCAGACCAT+AGG 0.618883 3.2:-15543590 None:intergenic
CTCCGGAGATGATCCAACAT+AGG 0.651094 3.2:+15543857 MS.gene94531:CDS
AATGATAAAATGTCAGCTGG+AGG 0.651490 3.2:+15543505 None:intergenic
TTCTCCGTGAAATAATGACA+AGG 0.688796 3.2:+15544830 MS.gene94531:CDS
TGTACCAGACCATAGGCTTG+CGG 0.689736 3.2:-15543583 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTTATAACAAAAAATTA+AGG - chr3.2:15544530-15544549 None:intergenic 10.0%
!!! AAAGAATAATAATTTTTTTG+GGG - chr3.2:15544014-15544033 None:intergenic 10.0%
!!! AAATTATAATTTTTTAGGTT+TGG - chr3.2:15544635-15544654 None:intergenic 10.0%
!! ATTATTATTCTTTAATTAGC+TGG + chr3.2:15544021-15544040 MS.gene94531:intron 15.0%
!! ATTGATAGATTTATTTGTAT+TGG + chr3.2:15544151-15544170 MS.gene94531:intron 15.0%
!! CTAAAATTAAACAAAATCTT+AGG - chr3.2:15544506-15544525 None:intergenic 15.0%
!! CTTTCAAAAAAAAAATGTAT+TGG + chr3.2:15544421-15544440 MS.gene94531:intron 15.0%
!! TAGTCATTTATAGATAAAAT+TGG + chr3.2:15544310-15544329 MS.gene94531:intron 15.0%
!! TATCTTTAAATTTAATACGT+TGG + chr3.2:15544675-15544694 MS.gene94531:intron 15.0%
!!! AGTTTTTTTTATTGTTGAAA+AGG - chr3.2:15544215-15544234 None:intergenic 15.0%
!!! CAATACATTTTTTTTTTGAA+AGG - chr3.2:15544423-15544442 None:intergenic 15.0%
!! ACAATAGCTATTTAAAAAGT+TGG - chr3.2:15544356-15544375 None:intergenic 20.0%
!! AGCTCAATGTTTATTATTTA+GGG - chr3.2:15544067-15544086 None:intergenic 20.0%
!! ATGGAATAAATTTATTTGCA+AGG + chr3.2:15544608-15544627 MS.gene94531:intron 20.0%
!! TAGCTCAATGTTTATTATTT+AGG - chr3.2:15544068-15544087 None:intergenic 20.0%
!! TATTTAAAAAGTTGGACAAA+AGG - chr3.2:15544348-15544367 None:intergenic 20.0%
!! TTAAAAAATCGAGACAAAAA+TGG + chr3.2:15544589-15544608 MS.gene94531:intron 20.0%
!!! ATTTTTCAATGTACACAAAT+AGG - chr3.2:15544267-15544286 None:intergenic 20.0%
!!! TAGATTTATTTGTATTGGTT+TGG + chr3.2:15544156-15544175 MS.gene94531:intron 20.0%
! ATTGCTATAATTTGAGCAAA+TGG - chr3.2:15544886-15544905 None:intergenic 25.0%
! TTGAGTTAACTTTCATGTAT+TGG + chr3.2:15544389-15544408 MS.gene94531:intron 25.0%
AAACAATCATTGGGTAAGAT+TGG - chr3.2:15544805-15544824 None:intergenic 30.0%
AACATAGTTCATTTCATAGG+TGG + chr3.2:15543557-15543576 MS.gene94531:CDS 30.0%
ACATCTACTTTAAGTGTGTA+AGG - chr3.2:15543883-15543902 None:intergenic 30.0%
ACCTATGAAATGAACTATGT+TGG - chr3.2:15543558-15543577 None:intergenic 30.0%
ACTTGTCACCAAAAATCAAA+AGG - chr3.2:15543750-15543769 None:intergenic 30.0%
AGCTGGTATGTATTTATATC+CGG + chr3.2:15544038-15544057 MS.gene94531:intron 30.0%
CAGTTGTCAATAAAAATGTC+TGG + chr3.2:15544779-15544798 MS.gene94531:CDS 30.0%
CCTATGAAATGAACTATGTT+GGG - chr3.2:15543557-15543576 None:intergenic 30.0%
! AGCTAGGAAAAATTTTATGC+AGG - chr3.2:15544726-15544745 None:intergenic 30.0%
! CTAGGAAAAATTTTATGCAG+GGG - chr3.2:15544724-15544743 None:intergenic 30.0%
! GCTAGGAAAAATTTTATGCA+GGG - chr3.2:15544725-15544744 None:intergenic 30.0%
! TGTTTATTATTTAGGGTAGC+CGG - chr3.2:15544060-15544079 None:intergenic 30.0%
! TTCGGTAACTAAACTTGTTT+GGG - chr3.2:15544092-15544111 None:intergenic 30.0%
! TTGTTTGACAGCTAGTTTAA+GGG - chr3.2:15543653-15543672 None:intergenic 30.0%
! TTTCGGTAACTAAACTTGTT+TGG - chr3.2:15544093-15544112 None:intergenic 30.0%
!! ATAGCTTTATGATTGTTCTC+TGG + chr3.2:15543905-15543924 MS.gene94531:CDS 30.0%
!! TAGCTTTATGATTGTTCTCT+GGG + chr3.2:15543906-15543925 MS.gene94531:CDS 30.0%
!! TCAATCAAAATTGCTGACTT+TGG + chr3.2:15543769-15543788 MS.gene94531:CDS 30.0%
!!! AAAAACGGTGTTTTTAGTCA+TGG - chr3.2:15544558-15544577 None:intergenic 30.0%
!!! TCTGACAACCTTTTGATTTT+TGG + chr3.2:15543739-15543758 MS.gene94531:CDS 30.0%
AGAACAGGCAAACAATCATT+GGG - chr3.2:15544814-15544833 None:intergenic 35.0%
AGCACCTTGTCATTATTTCA+CGG - chr3.2:15544837-15544856 None:intergenic 35.0%
ATCGTTACTGAGAATGCTAA+GGG + chr3.2:15543605-15543624 MS.gene94531:intron 35.0%
CAAATGGTGGTTTAACATCA+GGG - chr3.2:15544870-15544889 None:intergenic 35.0%
CCCAACATAGTTCATTTCAT+AGG + chr3.2:15543554-15543573 MS.gene94531:CDS 35.0%
CGTATCCTTTCATTATGAAG+GGG + chr3.2:15543976-15543995 MS.gene94531:intron 35.0%
GCGTATCCTTTCATTATGAA+GGG + chr3.2:15543975-15543994 MS.gene94531:intron 35.0%
GCTATAATTTGAGCAAATGG+TGG - chr3.2:15544883-15544902 None:intergenic 35.0%
TTCTCCGTGAAATAATGACA+AGG + chr3.2:15544830-15544849 MS.gene94531:CDS 35.0%
! CTTGTTTGACAGCTAGTTTA+AGG - chr3.2:15543654-15543673 None:intergenic 35.0%
! TCGGTAACTAAACTTGTTTG+GGG - chr3.2:15544091-15544110 None:intergenic 35.0%
! TGAAATAATGACAAGGTGCT+GGG + chr3.2:15544837-15544856 MS.gene94531:CDS 35.0%
!!! TAATTTTTTTGGGGTAGCCA+AGG - chr3.2:15544005-15544024 None:intergenic 35.0%
ACTAGCTGTCAAACAAGCTT+TGG + chr3.2:15543657-15543676 MS.gene94531:intron 40.0%
ATTTCAGAACATGACAGCAC+AGG + chr3.2:15543951-15543970 MS.gene94531:intron 40.0%
CATCGTTACTGAGAATGCTA+AGG + chr3.2:15543604-15543623 MS.gene94531:intron 40.0%
GAGAACAGGCAAACAATCAT+TGG - chr3.2:15544815-15544834 None:intergenic 40.0%
GCAAATGGTGGTTTAACATC+AGG - chr3.2:15544871-15544890 None:intergenic 40.0%
GGCGTATCCTTTCATTATGA+AGG + chr3.2:15543974-15543993 MS.gene94531:intron 40.0%
GTCATTATTTCACGGAGAAC+AGG - chr3.2:15544829-15544848 None:intergenic 40.0%
GTGAAATAATGACAAGGTGC+TGG + chr3.2:15544836-15544855 MS.gene94531:CDS 40.0%
TAAGTGTGTAAGGCCTATGT+TGG - chr3.2:15543873-15543892 None:intergenic 40.0%
TCAAGACTGGAACATACTGT+TGG + chr3.2:15543830-15543849 MS.gene94531:CDS 40.0%
TTTCAGAACATGACAGCACA+GGG + chr3.2:15543952-15543971 MS.gene94531:intron 40.0%
!! TGTGCTGTCATGTTCTGAAA+TGG - chr3.2:15543952-15543971 None:intergenic 40.0%
AACATACTGTTGGATGGCTC+CGG + chr3.2:15543840-15543859 MS.gene94531:CDS 45.0%
ATGGCTTATGTTCACGGTCT+GGG + chr3.2:15543694-15543713 MS.gene94531:intron 45.0%
GAAGGAATGACTCTCAAGAC+TGG + chr3.2:15543817-15543836 MS.gene94531:CDS 45.0%
GACTGGAACATACTGTTGGA+TGG + chr3.2:15543834-15543853 MS.gene94531:CDS 45.0%
GGCTACCCCTTCATAATGAA+AGG - chr3.2:15543984-15544003 None:intergenic 45.0%
GTAACGATGTACCAGACCAT+AGG - chr3.2:15543593-15543612 None:intergenic 45.0%
GTTACTGAGAATGCTAAGGG+TGG + chr3.2:15543608-15543627 MS.gene94531:intron 45.0%
TCATTATGAAGGGGTAGCCT+TGG + chr3.2:15543985-15544004 MS.gene94531:intron 45.0%
TTCAGAACATGACAGCACAG+GGG + chr3.2:15543953-15543972 MS.gene94531:intron 45.0%
TTTGGATGTTGCAAGAGGCA+TGG + chr3.2:15543675-15543694 MS.gene94531:intron 45.0%
! AGAGGCATGGCTTATGTTCA+CGG + chr3.2:15543688-15543707 MS.gene94531:intron 45.0%
!! CAAGCTTTGGATGTTGCAAG+AGG + chr3.2:15543670-15543689 MS.gene94531:intron 45.0%
!!! CTTATAAATTATAATTTTTT+AGG - chr3.2:15544640-15544659 None:intergenic 5.0%
!!! TAAAGAATAATAATTTTTTT+GGG - chr3.2:15544015-15544034 None:intergenic 5.0%
!!! TTAAAGAATAATAATTTTTT+TGG - chr3.2:15544016-15544035 None:intergenic 5.0%
!!! TTAAATTTTACAAAAAAAAA+CGG - chr3.2:15544573-15544592 None:intergenic 5.0%
!!! TTTTAATTTTTTTTTATAGT+TGG + chr3.2:15544698-15544717 MS.gene94531:intron 5.0%
!!! TTTTTATTTATGTTATTTTT+CGG - chr3.2:15544110-15544129 None:intergenic 5.0%
CATGGCTTATGTTCACGGTC+TGG + chr3.2:15543693-15543712 MS.gene94531:intron 50.0%
CGGATTGAGGTGCAAACTGA+AGG + chr3.2:15543799-15543818 MS.gene94531:CDS 50.0%
CTCCGGAGATGATCCAACAT+AGG + chr3.2:15543857-15543876 MS.gene94531:CDS 50.0%
GGCCTATGTTGGATCATCTC+CGG - chr3.2:15543862-15543881 None:intergenic 50.0%
GTTCTCTGGGTGCTCATAAC+TGG + chr3.2:15543919-15543938 MS.gene94531:CDS 50.0%
TGCTTGTACTGCTGTCACAG+CGG - chr3.2:15544752-15544771 None:intergenic 50.0%
TGTACCAGACCATAGGCTTG+CGG - chr3.2:15543586-15543605 None:intergenic 50.0%
! ACGGTCTGGGATTGTTTCAC+AGG + chr3.2:15543707-15543726 MS.gene94531:intron 50.0%
! CGGTCTGGGATTGTTTCACA+GGG + chr3.2:15543708-15543727 MS.gene94531:intron 50.0%
!! TTGCTGACTTTGGAGTCGCT+CGG + chr3.2:15543779-15543798 MS.gene94531:CDS 50.0%
!! CTTTGGAGTCGCTCGGATTG+AGG + chr3.2:15543786-15543805 MS.gene94531:CDS 55.0%
AGGTGGATGCCGCAAGCCTA+TGG + chr3.2:15543574-15543593 MS.gene94531:CDS 60.0%
GATGCCGCAAGCCTATGGTC+TGG + chr3.2:15543579-15543598 MS.gene94531:CDS 60.0%
GCTGTCACAGCGGCGAAGCT+AGG - chr3.2:15544742-15544761 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 15543506 15544912 15543506 ID=MS.gene94531
chr3.2 mRNA 15543506 15544912 15543506 ID=MS.gene94531.t1;Parent=MS.gene94531
chr3.2 exon 15543506 15543600 15543506 ID=MS.gene94531.t1.exon1;Parent=MS.gene94531.t1
chr3.2 CDS 15543506 15543600 15543506 ID=cds.MS.gene94531.t1;Parent=MS.gene94531.t1
chr3.2 exon 15543729 15543940 15543729 ID=MS.gene94531.t1.exon2;Parent=MS.gene94531.t1
chr3.2 CDS 15543729 15543940 15543729 ID=cds.MS.gene94531.t1;Parent=MS.gene94531.t1
chr3.2 exon 15544761 15544912 15544761 ID=MS.gene94531.t1.exon3;Parent=MS.gene94531.t1
chr3.2 CDS 15544761 15544912 15544761 ID=cds.MS.gene94531.t1;Parent=MS.gene94531.t1
Gene Sequence

>MS.gene94531

ATGATAAAATGTCAGCTGGAGGTTATGATGCTTGCTACATTGAAGCATCCCAACATAGTTCATTTCATAGGTGGATGCCGCAAGCCTATGGTCTGGTACATCGTTACTGAGAATGCTAAGGGTGGATCAGTTCGACTCTTGTTTCCCTTAAACTAGCTGTCAAACAAGCTTTGGATGTTGCAAGAGGCATGGCTTATGTTCACGGTCTGGGATTGTTTCACAGGGACTTGAAATCTGACAACCTTTTGATTTTTGGTGACAAGTCAATCAAAATTGCTGACTTTGGAGTCGCTCGGATTGAGGTGCAAACTGAAGGAATGACTCTCAAGACTGGAACATACTGTTGGATGGCTCCGGAGATGATCCAACATAGGCCTTACACACTTAAAGTAGATGTATATAGCTTTATGATTGTTCTCTGGGTGCTCATAACTGGTATGCTTCCATTTCAGAACATGACAGCACAGGGGCGTATCCTTTCATTATGAAGGGGTAGCCTTGGCTACCCCAAAAAAATTATTATTCTTTAATTAGCTGGTATGTATTTATATCCGGCTACCCTAAATAATAAACATTGAGCTACCCCAAACAAGTTTAGTTACCGAAAAATAACATAAATAAAAAAGTAAAAAAAATTATTATATGATTGATAGATTTATTTGTATTGGTTTGGTACTTTCGCTCTCAATAACAATTATAGAGAGAACCTTTTCAACAATAAAAAAAACTAGCACAACAATAGTCATAATTAAACATAACCTATTTGTGTACATTGAAAAATATATTATTAATCTATTTTGTAAATAGTCATTTATAGATAAAATTGGCTTCTTGAAACACCTTTTGTCCAACTTTTTAAATAGCTATTGTCAATTTTTATCATTTGAGTTAACTTTCATGTATTGGTTAATTTTCCTTTCAAAAAAAAAATGTATTGGTTAATTTTCAGATTATTTTGATTTTAGTCTACAAAGCTTGCGATTTTTTTAGTTAAGTTCCTAAGATTTTGTTTAATTTTAGTCCTTAATTTTTTGTTATAAAGATGAAATCCATGACTAAAAACACCGTTTTTTTTTGTAAAATTTAAAAAATCGAGACAAAAATGGAATAAATTTATTTGCAAGGACCAAACCTAAAAAATTATAATTTATAAGACTAAAAATATATTTTATCTTTAAATTTAATACGTTGGTTTTAATTTTTTTTTATAGTTGGCCCCTGCATAAAATTTTTCCTAGCTTCGCCGCTGTGACAGCAGTACAAGCAGCATTTGCAGTTGTCAATAAAAATGTCTGGCCAATCTTACCCAATGATTGTTTGCCTGTTCTCCGTGAAATAATGACAAGGTGCTGGGATACAAACCCTGATGTTAAACCACCATTTGCTCAAATTATAGCAATGCTGTAA
Protein sequence

>MS.gene94531.t1

MIKCQLEVMMLATLKHPNIVHFIGGCRKPMVWDLKSDNLLIFGDKSIKIADFGVARIEVQTEGMTLKTGTYCWMAPEMIQHRPYTLKVDVYSFMIVLWVLITAVQAAFAVVNKNVWPILPNDCLPVLREIMTRCWDTNPDVKPPFAQIIAML