Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94560.t1 | XP_003631116.2 | 83.7 | 129 | 6 | 3 | 1 | 125 | 1 | 118 | 7.00E-48 | 199.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94560.t1 | Q8LBM2 | 42.0 | 131 | 64 | 6 | 1 | 124 | 3 | 128 | 1.9e-14 | 80.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94560.t1 | G7LE60 | 83.7 | 129 | 6 | 3 | 1 | 125 | 1 | 118 | 5.0e-48 | 199.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene94560.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94560.t1 | MTR_8g107300 | 83.721 | 129 | 6 | 3 | 1 | 125 | 1 | 118 | 2.49e-70 | 206 |
| MS.gene94560.t1 | MTR_2g019190 | 76.471 | 136 | 19 | 3 | 1 | 125 | 1 | 134 | 9.65e-68 | 200 |
| MS.gene94560.t1 | MTR_8g107300 | 82.979 | 94 | 5 | 1 | 32 | 125 | 1 | 83 | 1.01e-48 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94560.t1 | AT1G30135 | 41.912 | 136 | 57 | 7 | 1 | 124 | 3 | 128 | 2.13e-21 | 83.2 |
| MS.gene94560.t1 | AT2G34600 | 39.062 | 128 | 72 | 5 | 1 | 124 | 20 | 145 | 1.25e-16 | 71.2 |
Find 26 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTGATGTCACAGAGTTTC+AGG | 0.192920 | 4.1:+280699 | MS.gene94560:CDS |
| TGCCAGCATTAAGATGGATT+TGG | 0.344981 | 4.1:-280833 | None:intergenic |
| CAGAATCATAGAGAGGAAAA+AGG | 0.376693 | 4.1:-280460 | None:intergenic |
| CATCTTCCCATCATAGAATA+TGG | 0.389620 | 4.1:-280671 | None:intergenic |
| CCTCATCAATTGTATAGCCC+TGG | 0.405940 | 4.1:+280931 | MS.gene94560:CDS |
| GATGGTATGGAGATGCTTCT+TGG | 0.408900 | 4.1:-281014 | None:intergenic |
| GGAAAGCCCAATGCAAAATC+AGG | 0.420902 | 4.1:+280636 | MS.gene94560:CDS |
| TTTATTTGCCAGCATTAAGA+TGG | 0.422633 | 4.1:-280839 | None:intergenic |
| TTCTCTTATCTTACATGTGG+TGG | 0.433401 | 4.1:-280502 | None:intergenic |
| CCGCTGACCATATTCTATGA+TGG | 0.457336 | 4.1:+280664 | MS.gene94560:CDS |
| TCTACAGAAGAGAAAGAATA+GGG | 0.468849 | 4.1:+280990 | MS.gene94560:CDS |
| CTCATCAATTGTATAGCCCT+GGG | 0.470700 | 4.1:+280932 | MS.gene94560:CDS |
| TGAGGAGGCTGCACTATTGT+TGG | 0.477785 | 4.1:-280913 | None:intergenic |
| CAATTGTATAGCCCTGGGTC+TGG | 0.482257 | 4.1:+280937 | MS.gene94560:CDS |
| TTCTACAGAAGAGAAAGAAT+AGG | 0.508590 | 4.1:+280989 | MS.gene94560:CDS |
| CGCTGACCATATTCTATGAT+GGG | 0.509614 | 4.1:+280665 | MS.gene94560:CDS |
| GGCCAAATCCATCTTAATGC+TGG | 0.519970 | 4.1:+280831 | MS.gene94560:intron |
| GCAAAGATCTCTTCATTGAA+AGG | 0.520559 | 4.1:-280960 | None:intergenic |
| TTCATTGAAAGGCCAGACCC+AGG | 0.532257 | 4.1:-280949 | None:intergenic |
| GTTGTTCGCTCTGACCCTCT+CGG | 0.542596 | 4.1:-280889 | None:intergenic |
| CAATGAAGAGATCTTTGCAA+CGG | 0.550495 | 4.1:+280965 | MS.gene94560:CDS |
| GGGCTATACAATTGATGAGG+AGG | 0.602793 | 4.1:-280928 | None:intergenic |
| TCATTGAAAGGCCAGACCCA+GGG | 0.615225 | 4.1:-280948 | None:intergenic |
| CCAGGGCTATACAATTGATG+AGG | 0.668057 | 4.1:-280931 | None:intergenic |
| CCATCATAGAATATGGTCAG+CGG | 0.671722 | 4.1:-280664 | None:intergenic |
| TGATTGCCAGAATCATAGAG+AGG | 0.688733 | 4.1:-280467 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGATTTTCTTTTTATATTT+GGG | + | chr4.1:280570-280589 | MS.gene94560:intron | 10.0% |
| !!! | TATGATTTTCTTTTTATATT+TGG | + | chr4.1:280569-280588 | MS.gene94560:intron | 10.0% |
| !!! | TCATTTTTTCATTTAGTTAT+TGG | - | chr4.1:280758-280777 | None:intergenic | 15.0% |
| !! | ACAAATTGAAAAAATGGAAA+TGG | - | chr4.1:280544-280563 | None:intergenic | 20.0% |
| !! | AGTCTAACAAATTGAAAAAA+TGG | - | chr4.1:280550-280569 | None:intergenic | 20.0% |
| ! | TAATTTCTATCTCCATTGAA+GGG | + | chr4.1:280612-280631 | MS.gene94560:intron | 25.0% |
| ! | TTAATTTCTATCTCCATTGA+AGG | + | chr4.1:280611-280630 | MS.gene94560:intron | 25.0% |
| TCTACAGAAGAGAAAGAATA+GGG | + | chr4.1:280990-281009 | MS.gene94560:CDS | 30.0% | |
| TTCTACAGAAGAGAAAGAAT+AGG | + | chr4.1:280989-281008 | MS.gene94560:CDS | 30.0% | |
| TTTATTTGCCAGCATTAAGA+TGG | - | chr4.1:280842-280861 | None:intergenic | 30.0% | |
| CAATGAAGAGATCTTTGCAA+CGG | + | chr4.1:280965-280984 | MS.gene94560:CDS | 35.0% | |
| CAGAATCATAGAGAGGAAAA+AGG | - | chr4.1:280463-280482 | None:intergenic | 35.0% | |
| CATCTTCCCATCATAGAATA+TGG | - | chr4.1:280674-280693 | None:intergenic | 35.0% | |
| GCAAAGATCTCTTCATTGAA+AGG | - | chr4.1:280963-280982 | None:intergenic | 35.0% | |
| GCATTCTCTTATCTTACATG+TGG | - | chr4.1:280508-280527 | None:intergenic | 35.0% | |
| TTCTCTTATCTTACATGTGG+TGG | - | chr4.1:280505-280524 | None:intergenic | 35.0% | |
| ! | CTTTTTCCTCTCTATGATTC+TGG | + | chr4.1:280461-280480 | MS.gene94560:CDS | 35.0% |
| AAAAAGTGAAGACACCGAGA+GGG | + | chr4.1:280875-280894 | MS.gene94560:CDS | 40.0% | |
| CCATCATAGAATATGGTCAG+CGG | - | chr4.1:280667-280686 | None:intergenic | 40.0% | |
| CGCTGACCATATTCTATGAT+GGG | + | chr4.1:280665-280684 | MS.gene94560:CDS | 40.0% | |
| CTCATCAATTGTATAGCCCT+GGG | + | chr4.1:280932-280951 | MS.gene94560:CDS | 40.0% | |
| GGTTAATGTGAATGTTGTGC+AGG | + | chr4.1:280810-280829 | MS.gene94560:intron | 40.0% | |
| TGATTGCCAGAATCATAGAG+AGG | - | chr4.1:280470-280489 | None:intergenic | 40.0% | |
| TTTCTATCTCCATTGAAGGG+AGG | + | chr4.1:280615-280634 | MS.gene94560:intron | 40.0% | |
| ! | GCTGTTCCTGATTTTGCATT+GGG | - | chr4.1:280645-280664 | None:intergenic | 40.0% |
| ! | TGCCAGCATTAAGATGGATT+TGG | - | chr4.1:280836-280855 | None:intergenic | 40.0% |
| CCAGGGCTATACAATTGATG+AGG | - | chr4.1:280934-280953 | None:intergenic | 45.0% | |
| CCGCTGACCATATTCTATGA+TGG | + | chr4.1:280664-280683 | MS.gene94560:CDS | 45.0% | |
| CCTCATCAATTGTATAGCCC+TGG | + | chr4.1:280931-280950 | MS.gene94560:CDS | 45.0% | |
| GAAAAAGTGAAGACACCGAG+AGG | + | chr4.1:280874-280893 | MS.gene94560:CDS | 45.0% | |
| GACTGATGTCACAGAGTTTC+AGG | + | chr4.1:280699-280718 | MS.gene94560:CDS | 45.0% | |
| GATGGTATGGAGATGCTTCT+TGG | - | chr4.1:281017-281036 | None:intergenic | 45.0% | |
| GGAAAGCCCAATGCAAAATC+AGG | + | chr4.1:280636-280655 | MS.gene94560:CDS | 45.0% | |
| GGCCAAATCCATCTTAATGC+TGG | + | chr4.1:280831-280850 | MS.gene94560:intron | 45.0% | |
| GGGCTATACAATTGATGAGG+AGG | - | chr4.1:280931-280950 | None:intergenic | 45.0% | |
| TTGGCCTAGTCAAGAACAGA+GGG | - | chr4.1:280739-280758 | None:intergenic | 45.0% | |
| ! | ATTGGCCTAGTCAAGAACAG+AGG | - | chr4.1:280740-280759 | None:intergenic | 45.0% |
| ! | GGCTGTTCCTGATTTTGCAT+TGG | - | chr4.1:280646-280665 | None:intergenic | 45.0% |
| !! | CTTTATAAAAATAAATAAAT+GGG | + | chr4.1:280789-280808 | MS.gene94560:intron | 5.0% |
| !! | TCTTTATAAAAATAAATAAA+TGG | + | chr4.1:280788-280807 | MS.gene94560:intron | 5.0% |
| CAATTGTATAGCCCTGGGTC+TGG | + | chr4.1:280937-280956 | MS.gene94560:CDS | 50.0% | |
| TCATTGAAAGGCCAGACCCA+GGG | - | chr4.1:280951-280970 | None:intergenic | 50.0% | |
| TGAGGAGGCTGCACTATTGT+TGG | - | chr4.1:280916-280935 | None:intergenic | 50.0% | |
| TTCATTGAAAGGCCAGACCC+AGG | - | chr4.1:280952-280971 | None:intergenic | 50.0% | |
| TTGGGCTTTCCTCCCTTCAA+TGG | - | chr4.1:280627-280646 | None:intergenic | 50.0% | |
| ACCGCCCTCTGTTCTTGACT+AGG | + | chr4.1:280732-280751 | MS.gene94560:intron | 55.0% | |
| GCCTAGTCAAGAACAGAGGG+CGG | - | chr4.1:280736-280755 | None:intergenic | 55.0% | |
| GTTGTTCGCTCTGACCCTCT+CGG | - | chr4.1:280892-280911 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 280431 | 281041 | 280431 | ID=MS.gene94560 |
| chr4.1 | mRNA | 280431 | 281041 | 280431 | ID=MS.gene94560.t1;Parent=MS.gene94560 |
| chr4.1 | exon | 280431 | 280510 | 280431 | ID=MS.gene94560.t1.exon1;Parent=MS.gene94560.t1 |
| chr4.1 | CDS | 280431 | 280510 | 280431 | ID=cds.MS.gene94560.t1;Parent=MS.gene94560.t1 |
| chr4.1 | exon | 280633 | 280720 | 280633 | ID=MS.gene94560.t1.exon2;Parent=MS.gene94560.t1 |
| chr4.1 | CDS | 280633 | 280720 | 280633 | ID=cds.MS.gene94560.t1;Parent=MS.gene94560.t1 |
| chr4.1 | exon | 280832 | 281041 | 280832 | ID=MS.gene94560.t1.exon3;Parent=MS.gene94560.t1 |
| chr4.1 | CDS | 280832 | 281041 | 280832 | ID=cds.MS.gene94560.t1;Parent=MS.gene94560.t1 |
| Gene Sequence |
| Protein sequence |
