AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94866

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94866.t1 MTR_6g053200 83.523 352 46 2 1 352 197 536 0.0 553
MS.gene94866.t1 MTR_2g024380 40.327 367 151 5 22 352 136 470 1.52e-74 238
MS.gene94866.t1 MTR_2g024350 47.137 227 119 1 127 352 1 227 4.13e-67 211
MS.gene94866.t1 MTR_8g067650 39.450 327 143 8 38 348 229 516 1.23e-59 200
MS.gene94866.t1 MTR_8g067635 37.166 374 151 7 15 337 181 521 7.42e-57 193
MS.gene94866.t1 MTR_8g069935 52.778 180 70 1 38 202 220 399 2.45e-53 182
MS.gene94866.t1 MTR_7g104980 49.749 199 98 2 155 352 18 215 6.31e-49 164
MS.gene94866.t1 MTR_1g099600 40.800 125 63 2 85 209 146 259 2.73e-27 109
MS.gene94866.t1 MTR_8g028565 44.660 103 54 2 111 213 134 233 3.43e-23 99.0
MS.gene94866.t1 MTR_7g079040 39.552 134 67 4 106 231 118 245 7.21e-23 97.8
MS.gene94866.t1 MTR_7g105000 39.474 152 73 4 35 171 209 356 2.97e-22 96.7
MS.gene94866.t1 MTR_7g109600 44.706 85 47 0 127 211 46 130 1.31e-19 87.4
MS.gene94866.t1 MTR_2g032030 48.052 77 39 1 135 211 268 343 2.71e-19 89.0
MS.gene94866.t1 MTR_2g032030 48.052 77 39 1 135 211 268 343 6.91e-19 87.8
MS.gene94866.t1 MTR_8g098945 50.000 72 35 1 135 206 130 200 2.62e-18 84.0
MS.gene94866.t1 MTR_7g062220 40.566 106 51 3 86 191 87 180 1.14e-16 80.1
MS.gene94866.t1 MTR_5g016610 44.706 85 44 1 110 191 172 256 1.93e-16 78.6
MS.gene94866.t1 MTR_4g127040 44.706 85 44 1 110 191 218 302 3.78e-16 78.6
MS.gene94866.t1 MTR_2g083870 50.794 63 31 0 135 197 81 143 9.26e-16 76.6
MS.gene94866.t1 MTR_2g016770 50.000 60 30 0 135 194 28 87 1.21e-15 75.1
MS.gene94866.t1 MTR_8g087000 47.619 63 33 0 135 197 78 140 1.27e-15 75.9
MS.gene94866.t1 MTR_8g087000 47.619 63 33 0 135 197 77 139 1.33e-15 75.9
MS.gene94866.t1 MTR_2g016770 50.000 60 30 0 135 194 36 95 1.35e-15 75.1
MS.gene94866.t1 MTR_7g011820 42.683 82 45 2 132 213 408 487 2.09e-15 77.4
MS.gene94866.t1 MTR_1g070455 45.122 82 42 1 115 193 271 352 2.19e-15 76.6
MS.gene94866.t1 MTR_4g094908 51.613 62 30 0 135 196 267 328 2.67e-15 76.3
MS.gene94866.t1 MTR_4g082580 39.785 93 51 2 132 220 276 367 7.19e-15 75.5
MS.gene94866.t1 MTR_0117s0040 39.506 81 49 0 128 208 68 148 1.05e-14 73.2
MS.gene94866.t1 MTR_1g009613 34.737 95 57 1 114 203 237 331 1.39e-14 74.3
MS.gene94866.t1 MTR_3g108960 37.778 90 55 1 103 191 282 371 1.90e-14 74.3
MS.gene94866.t1 MTR_8g099350 38.043 92 51 2 104 189 98 189 1.96e-14 73.9
MS.gene94866.t1 MTR_5g042920 43.478 69 38 1 132 200 318 385 3.64e-14 73.6
MS.gene94866.t1 MTR_1g077780 36.842 95 52 3 127 219 53 141 3.03e-13 67.0
MS.gene94866.t1 MTR_4g007060 42.353 85 48 1 129 212 123 207 4.19e-13 69.3
MS.gene94866.t1 MTR_4g007060 42.353 85 48 1 129 212 157 241 4.82e-13 69.3
MS.gene94866.t1 MTR_2g045360 43.333 60 34 0 135 194 212 271 1.05e-12 68.9
MS.gene94866.t1 MTR_7g100510 44.615 65 35 1 130 194 357 420 1.14e-12 68.9
MS.gene94866.t1 MTR_3g106060 40.476 84 49 1 109 191 112 195 1.24e-12 68.2
MS.gene94866.t1 MTR_7g080020 46.377 69 35 2 126 194 272 338 1.72e-12 68.9
MS.gene94866.t1 MTR_7g080020 42.424 66 37 1 134 199 493 557 3.39e-12 68.2
MS.gene94866.t1 MTR_4g094588 45.902 61 32 1 134 194 178 237 1.88e-12 67.8
MS.gene94866.t1 MTR_3g095040 40.580 69 38 3 131 198 115 181 2.65e-12 65.5
MS.gene94866.t1 MTR_2g023930 33.981 103 64 3 93 194 266 365 2.78e-12 68.2
MS.gene94866.t1 MTR_5g091390 46.774 62 32 1 134 195 157 217 3.45e-12 67.0
MS.gene94866.t1 MTR_2g105060 43.284 67 38 0 129 195 89 155 3.48e-12 65.9
MS.gene94866.t1 MTR_4g130900 30.702 114 73 3 85 197 313 421 4.79e-12 67.4
MS.gene94866.t1 MTR_7g071120 47.059 68 34 2 127 194 159 224 5.37e-12 67.0
MS.gene94866.t1 MTR_3g056100 38.806 67 40 1 134 200 415 480 5.82e-12 67.0
MS.gene94866.t1 MTR_3g056100 38.806 67 40 1 134 200 402 467 6.06e-12 67.0
MS.gene94866.t1 MTR_3g056100 38.806 67 40 1 134 200 258 323 6.32e-12 66.6
MS.gene94866.t1 MTR_7g080000 42.188 64 36 1 134 197 432 494 6.39e-12 67.0
MS.gene94866.t1 MTR_3g009470 39.062 64 38 1 134 197 495 557 6.87e-12 67.0
MS.gene94866.t1 MTR_3g009470 50.000 62 29 2 133 194 279 338 7.58e-12 67.0
MS.gene94866.t1 MTR_3g056100 38.806 67 40 1 134 200 271 336 7.12e-12 66.6
MS.gene94866.t1 MTR_5g094430 38.806 67 40 1 134 200 364 429 7.15e-12 67.0
MS.gene94866.t1 MTR_2g105060 43.284 67 38 0 129 195 164 230 7.93e-12 65.9
MS.gene94866.t1 MTR_1g086790 40.000 70 41 1 134 203 158 226 9.31e-12 65.9
MS.gene94866.t1 MTR_5g094430 38.806 67 40 1 134 200 230 295 9.52e-12 66.2
MS.gene94866.t1 MTR_5g094430 38.806 67 40 1 134 200 252 317 9.73e-12 66.2
MS.gene94866.t1 MTR_1g013790 33.884 121 72 2 100 215 65 182 1.07e-11 64.7
MS.gene94866.t1 MTR_3g009470 50.000 62 29 2 133 194 279 338 1.21e-11 66.2
MS.gene94866.t1 MTR_4g132430 30.189 106 63 3 91 194 91 187 2.11e-11 64.7
MS.gene94866.t1 MTR_4g094412 33.766 77 50 1 120 196 221 296 4.98e-11 64.3
MS.gene94866.t1 MTR_5g097490 41.892 74 39 2 127 197 242 314 5.21e-11 63.9
MS.gene94866.t1 MTR_4g094412 33.766 77 50 1 120 196 230 305 5.43e-11 63.9
MS.gene94866.t1 MTR_1g013760 40.278 72 43 0 135 206 141 212 5.82e-11 62.8
MS.gene94866.t1 MTR_4g013260 39.062 64 38 1 134 197 336 398 6.00e-11 63.9
MS.gene94866.t1 MTR_4g132430 30.189 106 63 3 91 194 225 321 6.04e-11 63.9
MS.gene94866.t1 MTR_5g074400 39.062 64 38 1 134 197 420 482 8.42e-11 63.5
MS.gene94866.t1 MTR_7g109800 44.262 61 33 1 134 194 145 204 9.03e-11 62.4
MS.gene94866.t1 MTR_2g033820 41.935 62 35 1 134 195 203 263 9.49e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94866.t1 AT5G52830 50.000 98 47 2 112 209 144 239 2.10e-25 105
MS.gene94866.t1 AT4G01250 35.526 152 90 3 78 224 74 222 7.40e-23 97.4
MS.gene94866.t1 AT4G23550 45.455 88 47 1 126 213 132 218 9.52e-21 91.7
MS.gene94866.t1 AT5G45050 39.091 110 65 2 94 203 1002 1109 3.51e-18 86.7
MS.gene94866.t1 AT5G45050 39.091 110 65 2 94 203 1141 1248 3.83e-18 86.7
MS.gene94866.t1 AT5G45050 39.091 110 65 2 94 203 1113 1220 4.26e-18 86.3
MS.gene94866.t1 AT1G30650 38.793 116 66 2 88 203 175 285 6.39e-18 84.7
MS.gene94866.t1 AT2G34830 50.725 69 33 1 135 203 216 283 1.10e-17 84.0
MS.gene94866.t1 AT2G34830 50.725 69 33 1 135 203 225 292 1.25e-17 84.0
MS.gene94866.t1 AT2G23320 43.529 85 45 1 110 191 213 297 2.87e-17 82.0
MS.gene94866.t1 AT5G28650 41.304 92 51 1 105 193 230 321 3.45e-17 81.6
MS.gene94866.t1 AT3G04670 41.304 92 51 1 105 193 230 321 4.87e-17 81.3
MS.gene94866.t1 AT4G24240 41.935 93 51 1 102 191 246 338 3.69e-16 79.0
MS.gene94866.t1 AT4G31550 38.000 100 59 1 103 199 211 310 1.96e-15 76.6
MS.gene94866.t1 AT4G31550 38.710 93 54 1 110 199 219 311 2.70e-15 76.3
MS.gene94866.t1 AT3G58710 46.970 66 35 0 135 200 72 137 3.97e-15 75.1
MS.gene94866.t1 AT3G58710 46.970 66 35 0 135 200 71 136 4.00e-15 75.1
MS.gene94866.t1 AT2G24570 39.080 87 50 1 110 193 216 302 6.34e-15 75.1
MS.gene94866.t1 AT1G29280 48.333 60 31 0 135 194 75 134 1.40e-14 73.2
MS.gene94866.t1 AT2G03340 43.478 69 38 1 132 200 413 480 2.73e-14 74.3
MS.gene94866.t1 AT2G30590 39.080 87 45 1 107 193 294 372 9.73e-14 72.0
MS.gene94866.t1 AT3G01080 36.559 93 48 2 112 194 312 403 1.53e-13 72.0
MS.gene94866.t1 AT1G80840 34.454 119 75 2 129 245 141 258 1.58e-13 70.9
MS.gene94866.t1 AT3G01080 36.559 93 48 2 112 194 274 365 1.97e-13 71.2
MS.gene94866.t1 AT5G43290 41.096 73 42 1 119 191 99 170 4.69e-13 68.9
MS.gene94866.t1 AT2G04880 44.444 63 34 1 135 197 284 345 8.69e-13 69.7
MS.gene94866.t1 AT5G45260 30.573 157 89 7 48 197 1130 1273 8.84e-13 70.1
MS.gene94866.t1 AT2G04880 44.444 63 34 1 135 197 308 369 9.52e-13 69.3
MS.gene94866.t1 AT1G13960 42.857 63 35 1 132 194 254 315 1.67e-12 68.2
MS.gene94866.t1 AT1G13960 42.857 63 35 1 132 194 407 468 1.74e-12 68.6
MS.gene94866.t1 AT4G31800 42.500 80 43 1 129 205 106 185 2.19e-12 66.6
MS.gene94866.t1 AT4G31800 42.500 80 43 1 129 205 171 250 4.73e-12 66.6
MS.gene94866.t1 AT2G25000 45.455 66 36 0 129 194 98 163 4.88e-12 65.5
MS.gene94866.t1 AT2G25000 45.455 66 36 0 129 194 98 163 4.88e-12 65.5
MS.gene94866.t1 AT4G18170 35.577 104 61 2 96 194 129 231 5.30e-12 66.2
MS.gene94866.t1 AT2G25000 45.455 66 36 0 129 194 120 185 5.72e-12 65.5
MS.gene94866.t1 AT2G47260 38.636 88 52 2 107 194 148 233 5.75e-12 66.6
MS.gene94866.t1 AT1G29860 43.548 62 34 1 134 195 136 196 9.20e-12 65.5
MS.gene94866.t1 AT5G56270 39.062 64 38 1 134 197 487 549 1.24e-11 66.2
MS.gene94866.t1 AT2G25000 45.455 66 36 0 129 194 141 206 1.26e-11 64.7
MS.gene94866.t1 AT4G26640 41.250 80 44 3 130 208 167 244 1.36e-11 65.9
MS.gene94866.t1 AT4G26640 34.568 81 46 2 134 214 341 414 9.48e-11 63.5
MS.gene94866.t1 AT4G26640 42.308 78 42 3 130 206 31 106 1.36e-11 65.5
MS.gene94866.t1 AT4G26640 34.568 81 46 2 134 214 205 278 9.19e-11 63.2
MS.gene94866.t1 AT4G26640 42.308 78 42 3 130 206 31 106 1.36e-11 65.5
MS.gene94866.t1 AT4G26640 34.568 81 46 2 134 214 205 278 9.19e-11 63.2
MS.gene94866.t1 AT4G26640 42.308 78 42 3 130 206 31 106 1.36e-11 65.5
MS.gene94866.t1 AT4G26640 34.568 81 46 2 134 214 205 278 9.19e-11 63.2
MS.gene94866.t1 AT4G26640 42.308 78 42 3 130 206 31 106 1.36e-11 65.5
MS.gene94866.t1 AT4G26640 34.568 81 46 2 134 214 205 278 9.19e-11 63.2
MS.gene94866.t1 AT4G26640 42.308 78 42 3 130 206 207 282 1.38e-11 66.2
MS.gene94866.t1 AT4G26640 42.308 78 42 3 130 206 135 210 1.38e-11 65.9
MS.gene94866.t1 AT4G26640 34.568 81 46 2 134 214 309 382 9.64e-11 63.2
MS.gene94866.t1 AT2G37260 37.037 81 46 2 115 191 326 405 1.50e-11 65.5
MS.gene94866.t1 AT2G37260 37.037 81 46 2 115 191 244 323 1.58e-11 65.1
MS.gene94866.t1 AT2G37260 37.037 81 46 2 115 191 246 325 1.58e-11 65.1
MS.gene94866.t1 AT2G21900 45.161 62 33 1 134 195 109 169 2.28e-11 62.8
MS.gene94866.t1 AT2G38470 32.710 107 69 2 134 238 362 467 2.63e-11 65.1
MS.gene94866.t1 AT2G46400 28.342 187 113 3 104 283 69 241 3.00e-11 63.9
MS.gene94866.t1 AT2G40750 39.080 87 43 3 107 189 129 209 3.06e-11 64.3
MS.gene94866.t1 AT5G07100 43.939 66 36 1 129 194 19 83 3.43e-11 62.8
MS.gene94866.t1 AT5G07100 43.939 66 36 1 129 194 19 83 3.43e-11 62.8
MS.gene94866.t1 AT5G07100 43.939 66 36 1 129 194 19 83 3.43e-11 62.8
MS.gene94866.t1 AT5G07100 43.939 66 36 1 129 194 19 83 3.43e-11 62.8
MS.gene94866.t1 AT5G07100 43.939 66 36 1 129 194 112 176 3.48e-11 63.9
MS.gene94866.t1 AT1G68150 27.465 142 77 2 55 196 182 297 3.58e-11 64.3
MS.gene94866.t1 AT4G30935 36.364 66 41 1 132 197 329 393 3.79e-11 64.7
MS.gene94866.t1 AT4G11070 37.255 102 58 2 94 189 65 166 4.46e-11 63.2
MS.gene94866.t1 AT5G46350 38.235 68 41 1 134 201 183 249 4.82e-11 63.5
MS.gene94866.t1 AT4G11070 37.255 102 58 2 94 189 97 198 5.71e-11 63.2
MS.gene94866.t1 AT4G23810 40.000 85 45 2 111 189 131 215 6.00e-11 63.2
MS.gene94866.t1 AT1G80590 44.304 79 41 2 135 211 86 163 7.28e-11 62.0
MS.gene94866.t1 AT3G56400 37.273 110 55 5 94 197 88 189 7.80e-11 62.8
MS.gene94866.t1 AT1G69310 42.373 59 33 1 134 192 147 204 8.03e-11 62.8
MS.gene94866.t1 AT1G69310 42.373 59 33 1 134 192 147 204 8.03e-11 62.8
MS.gene94866.t1 AT1G69310 42.373 59 33 1 134 192 147 204 8.03e-11 62.8
MS.gene94866.t1 AT1G55600 45.000 60 32 1 135 194 308 366 9.55e-11 63.2

Find 73 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCAGAAAACAAACTTAT+TGG 0.125564 6.1:+29566536 MS.gene94866:CDS
TTTCTCTAGCAGCGGGTAAA+TGG 0.192922 6.1:-29566400 None:intergenic
TACCCGCTGTTGGAAATTTC+AGG 0.250971 6.1:+29566246 MS.gene94866:CDS
TGTGATTGATTGAGCTTTCT+TGG 0.276762 6.1:-29565101 None:intergenic
ACTTGAATATTCCTGCTCTT+TGG 0.289651 6.1:-29566329 None:intergenic
TGGTGGTCGTGGACAAAATA+TGG 0.290490 6.1:+29565365 MS.gene94866:CDS
TAGGCTTTGATGACTTGTTC+TGG 0.297861 6.1:-29566220 None:intergenic
GAAATAGAAAACCATCATTT+AGG 0.308861 6.1:-29566637 None:intergenic
TATGTCAGAGGATTTCTTAT+TGG 0.311118 6.1:+29566604 MS.gene94866:CDS
AACATTATCACTAGTGTTCA+TGG 0.339452 6.1:+29564954 MS.gene94866:CDS
GTGATTGATTGAGCTTTCTT+GGG 0.361329 6.1:-29565100 None:intergenic
TTTCCAACAGCGGGTAATCT+AGG 0.362673 6.1:-29566239 None:intergenic
AGGCTTTGATGACTTGTTCT+GGG 0.370593 6.1:-29566219 None:intergenic
AATTAACAACAAGGTAACTT+TGG 0.407679 6.1:+29565307 MS.gene94866:CDS
GTCAAGTTGGTGGTGGTGCT+AGG 0.408314 6.1:-29564930 None:intergenic
AAGGCGTTGGTAGTGGTCAT+GGG 0.410054 6.1:-29564855 None:intergenic
AATTACCCAACACCAGATCT+AGG 0.412137 6.1:+29565129 MS.gene94866:CDS
CAAAACATAATTCAAGAATC+AGG 0.420705 6.1:+29565180 MS.gene94866:CDS
CAAGCAAGCTTTCAGAAGAT+TGG 0.420791 6.1:+29565345 MS.gene94866:CDS
TTCTCTAGCAGCGGGTAAAT+GGG 0.428823 6.1:-29566399 None:intergenic
AACACTTGACTGTTATTGTT+TGG 0.445737 6.1:-29565038 None:intergenic
GAAGGCGTTGGTAGTGGTCA+TGG 0.446538 6.1:-29564856 None:intergenic
GAACCCGGCTTTGAAATGTT+TGG 0.453733 6.1:-29566275 None:intergenic
AGTGATAATGTTAGTCAAGT+TGG 0.462717 6.1:-29564943 None:intergenic
TCATGAGCATTGCTACTCAT+TGG 0.466745 6.1:-29566488 None:intergenic
GCAGGTAAATGGAACTCTAT+TGG 0.471063 6.1:+29566188 MS.gene94866:CDS
ATTAGGTAAAATCCTAGATC+TGG 0.473232 6.1:-29565141 None:intergenic
GTGGTGGTGCTAGGGCTAAT+AGG 0.483423 6.1:-29564921 None:intergenic
GTCACCAAACATTTCAAAGC+CGG 0.485938 6.1:+29566271 MS.gene94866:CDS
ATAGAGTTCCATTTACCTGC+TGG 0.487185 6.1:-29566185 None:intergenic
GTTAGCGAAGGCGTTGGTAG+TGG 0.498129 6.1:-29564862 None:intergenic
TCAAGTTGGTGGTGGTGCTA+GGG 0.503913 6.1:-29564929 None:intergenic
GAAGGGATTTCTGATTGACT+AGG 0.514003 6.1:-29566356 None:intergenic
GATTCAATTTCTCTAGCAGC+GGG 0.517766 6.1:-29566407 None:intergenic
TCTATAAGTTCAGTTTCTGA+AGG 0.522348 6.1:-29566374 None:intergenic
TCACCAAACATTTCAAAGCC+GGG 0.523908 6.1:+29566272 MS.gene94866:CDS
AAGCCTAGATTACCCGCTGT+TGG 0.524427 6.1:+29566236 MS.gene94866:CDS
AAAATCCTAGATCTGGTGTT+GGG 0.524682 6.1:-29565134 None:intergenic
AAGGGATTTCTGATTGACTA+GGG 0.535133 6.1:-29566355 None:intergenic
GAGTATTGCATAATCTGAAA+TGG 0.538274 6.1:-29564994 None:intergenic
CACAACAAAAGTGTCCTCTT+CGG 0.542763 6.1:-29566132 None:intergenic
AGGAAGGATTTGTGATTCAC+CGG 0.548075 6.1:-29566516 None:intergenic
GCAAGCTTTCAGAAGATTGG+TGG 0.554578 6.1:+29565348 MS.gene94866:CDS
TCTGACATATCAGTCATGCT+TGG 0.560481 6.1:-29566590 None:intergenic
TGATTGATTGAGCTTTCTTG+GGG 0.561989 6.1:-29565099 None:intergenic
TAAAATCCTAGATCTGGTGT+TGG 0.562048 6.1:-29565135 None:intergenic
ATGTCGATCATCCAAAGAGC+AGG 0.571282 6.1:+29566318 MS.gene94866:CDS
TTTCAGAAGATTGGTGGTCG+TGG 0.572428 6.1:+29565354 MS.gene94866:CDS
ATGTGAAAAGACCTTGGATG+TGG 0.577521 6.1:-29565410 None:intergenic
CTATAAGTTCAGTTTCTGAA+GGG 0.578383 6.1:-29566373 None:intergenic
TGAAATGGTAGGATATTGAC+TGG 0.578765 6.1:-29564979 None:intergenic
AAAGCTTGCTTGTTCTCACA+TGG 0.591876 6.1:-29565334 None:intergenic
GGTAAATGGAACTCTATTGG+TGG 0.596193 6.1:+29566191 MS.gene94866:CDS
ATTGCATAATCTGAAATGGT+AGG 0.596503 6.1:-29564990 None:intergenic
CTAATAGGTCCAGTGGTGGT+CGG 0.599128 6.1:-29564906 None:intergenic
TGTGGGGTTAGCGAAGGCGT+TGG 0.601308 6.1:-29564868 None:intergenic
GACCTGAAATTTCCAACAGC+GGG 0.604025 6.1:-29566248 None:intergenic
TGATTCAATTTCTCTAGCAG+CGG 0.608307 6.1:-29566408 None:intergenic
CTTTGATGACTTGTTCTGGG+AGG 0.611922 6.1:-29566216 None:intergenic
ATAGAAAACCATCATTTAGG+TGG 0.614973 6.1:-29566634 None:intergenic
GTAAATGGAACTCTATTGGT+GGG 0.619020 6.1:+29566192 MS.gene94866:CDS
GCTAGGGCTAATAGGTCCAG+TGG 0.619531 6.1:-29564913 None:intergenic
TTAGCGGGTCCCCACATCCA+AGG 0.623038 6.1:+29565399 MS.gene94866:CDS
TGTGAAAAGACCTTGGATGT+GGG 0.630725 6.1:-29565409 None:intergenic
GATAATGTTAGTCAAGTTGG+TGG 0.636625 6.1:-29564940 None:intergenic
AGCAATGCTCATGATTACTC+CGG 0.637217 6.1:+29566497 MS.gene94866:CDS
GTGAAAAGACCTTGGATGTG+GGG 0.637723 6.1:-29565408 None:intergenic
AGTAGTTGTGGGGTTAGCGA+AGG 0.638079 6.1:-29564874 None:intergenic
AATGTTAGTCAAGTTGGTGG+TGG 0.642962 6.1:-29564937 None:intergenic
AAGCATGACTGATATGTCAG+AGG 0.660878 6.1:+29566592 MS.gene94866:CDS
GATTGATTGAGCTTTCTTGG+GGG 0.688353 6.1:-29565098 None:intergenic
AGGGCTAATAGGTCCAGTGG+TGG 0.699877 6.1:-29564910 None:intergenic
CGACCTGAAATTTCCAACAG+CGG 0.704425 6.1:-29566249 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATCAAAATTAATGTATTTT+TGG + chr6.1:29565707-29565726 MS.gene94866:intron 10.0%
!! ACTAAAAATATCTGTTTATT+TGG - chr6.1:29565972-29565991 None:intergenic 15.0%
!! AAGGATTAATATGAATATTC+AGG - chr6.1:29565882-29565901 None:intergenic 20.0%
!! ATTAAAGTGATATGATTTAG+AGG + chr6.1:29566006-29566025 MS.gene94866:intron 20.0%
!! TTACAGAAAAATTAACAACA+AGG + chr6.1:29565298-29565317 MS.gene94866:intron 20.0%
!!! TAACAATCAAAACTTTTACA+TGG - chr6.1:29565768-29565787 None:intergenic 20.0%
!!! TTTTTGGTTTAAGTACAAAA+TGG + chr6.1:29565723-29565742 MS.gene94866:intron 20.0%
! AATGAGTGATATATTTGTTC+AGG - chr6.1:29565908-29565927 None:intergenic 25.0%
! AATTAACAACAAGGTAACTT+TGG + chr6.1:29565307-29565326 MS.gene94866:CDS 25.0%
! ACAAATATATCACTCATTTC+AGG + chr6.1:29565910-29565929 MS.gene94866:intron 25.0%
! CAAAACATAATTCAAGAATC+AGG + chr6.1:29565180-29565199 MS.gene94866:CDS 25.0%
! CAAATATATCACTCATTTCA+GGG + chr6.1:29565911-29565930 MS.gene94866:intron 25.0%
! GAAATAGAAAACCATCATTT+AGG - chr6.1:29566640-29566659 None:intergenic 25.0%
! TAATACAAACATGATACCTT+GGG + chr6.1:29565496-29565515 MS.gene94866:intron 25.0%
!! CAATCTATGAATGATTTTTC+AGG + chr6.1:29566045-29566064 MS.gene94866:intron 25.0%
!! GAAAACAAGTTTTCAACATT+AGG - chr6.1:29566437-29566456 None:intergenic 25.0%
AACACTTGACTGTTATTGTT+TGG - chr6.1:29565041-29565060 None:intergenic 30.0%
AACATTATCACTAGTGTTCA+TGG + chr6.1:29564954-29564973 MS.gene94866:CDS 30.0%
ATAGAAAACCATCATTTAGG+TGG - chr6.1:29566637-29566656 None:intergenic 30.0%
ATTAGGTAAAATCCTAGATC+TGG - chr6.1:29565144-29565163 None:intergenic 30.0%
CCTTCAGAAAACAAACTTAT+TGG + chr6.1:29566536-29566555 MS.gene94866:CDS 30.0%
CTAATACAAACATGATACCT+TGG + chr6.1:29565495-29565514 MS.gene94866:intron 30.0%
CTATAAGTTCAGTTTCTGAA+GGG - chr6.1:29566376-29566395 None:intergenic 30.0%
GATATATTTGTTCAGGAACA+AGG - chr6.1:29565901-29565920 None:intergenic 30.0%
TATGGACAGAAAAACATTAG+CGG + chr6.1:29565383-29565402 MS.gene94866:CDS 30.0%
TATGTCAGAGGATTTCTTAT+TGG + chr6.1:29566604-29566623 MS.gene94866:CDS 30.0%
TCTATAAGTTCAGTTTCTGA+AGG - chr6.1:29566377-29566396 None:intergenic 30.0%
! AGTGATAATGTTAGTCAAGT+TGG - chr6.1:29564946-29564965 None:intergenic 30.0%
! ATTGCATAATCTGAAATGGT+AGG - chr6.1:29564993-29565012 None:intergenic 30.0%
! GAGTATTGCATAATCTGAAA+TGG - chr6.1:29564997-29565016 None:intergenic 30.0%
! GCTTTTTGTGAATCTGAATT+AGG - chr6.1:29565161-29565180 None:intergenic 30.0%
! TTTTGAATGTGAAAAGACCT+TGG - chr6.1:29565419-29565438 None:intergenic 30.0%
!! CCAATAAGTTTGTTTTCTGA+AGG - chr6.1:29566539-29566558 None:intergenic 30.0%
!! TAAGTTTGTTTTCTGAAGGA+AGG - chr6.1:29566535-29566554 None:intergenic 30.0%
!!! ATACCTTGGGTTTTAAATAG+TGG + chr6.1:29565509-29565528 MS.gene94866:intron 30.0%
AAAATCCTAGATCTGGTGTT+GGG - chr6.1:29565137-29565156 None:intergenic 35.0%
AAGACCACAATTCAGTGTAA+TGG - chr6.1:29565556-29565575 None:intergenic 35.0%
AAGGGATTTCTGATTGACTA+GGG - chr6.1:29566358-29566377 None:intergenic 35.0%
ACTTGAATATTCCTGCTCTT+TGG - chr6.1:29566332-29566351 None:intergenic 35.0%
ATATATCACTCATTTCAGGG+AGG + chr6.1:29565914-29565933 MS.gene94866:intron 35.0%
ATGAAAAACCAGCAGGTAAA+TGG + chr6.1:29566177-29566196 MS.gene94866:CDS 35.0%
ATGGACAGAAAAACATTAGC+GGG + chr6.1:29565384-29565403 MS.gene94866:CDS 35.0%
GAGTGAAAAAAGTAGTTGTG+GGG - chr6.1:29564887-29564906 None:intergenic 35.0%
GGAGTGAAAAAAGTAGTTGT+GGG - chr6.1:29564888-29564907 None:intergenic 35.0%
GTAAATGGAACTCTATTGGT+GGG + chr6.1:29566192-29566211 MS.gene94866:CDS 35.0%
GTGATTGATTGAGCTTTCTT+GGG - chr6.1:29565103-29565122 None:intergenic 35.0%
TAAAATCCTAGATCTGGTGT+TGG - chr6.1:29565138-29565157 None:intergenic 35.0%
TATATCACTCATTTCAGGGA+GGG + chr6.1:29565915-29565934 MS.gene94866:intron 35.0%
TGAAATGGTAGGATATTGAC+TGG - chr6.1:29564982-29565001 None:intergenic 35.0%
TGATTCAATTTCTCTAGCAG+CGG - chr6.1:29566411-29566430 None:intergenic 35.0%
TGATTGATTGAGCTTTCTTG+GGG - chr6.1:29565102-29565121 None:intergenic 35.0%
TGTGATTGATTGAGCTTTCT+TGG - chr6.1:29565104-29565123 None:intergenic 35.0%
! AGACCACTATTTAAAACCCA+AGG - chr6.1:29565515-29565534 None:intergenic 35.0%
! CTGAATTGTGGTCTTAATCT+AGG + chr6.1:29565561-29565580 MS.gene94866:intron 35.0%
!! ACTTTTGTTGTGACGTTTGT+TGG + chr6.1:29566143-29566162 MS.gene94866:CDS 35.0%
!! GATAATGTTAGTCAAGTTGG+TGG - chr6.1:29564943-29564962 None:intergenic 35.0%
!! GATTTTGACCACCTAAATGA+TGG + chr6.1:29566626-29566645 MS.gene94866:CDS 35.0%
!!! ATTTACCTGCTGGTTTTTCA+TGG - chr6.1:29566178-29566197 None:intergenic 35.0%
AAGCATGACTGATATGTCAG+AGG + chr6.1:29566592-29566611 MS.gene94866:CDS 40.0%
AATTACCCAACACCAGATCT+AGG + chr6.1:29565129-29565148 MS.gene94866:CDS 40.0%
ACAAAAAAGTGACACCGAAG+AGG + chr6.1:29566118-29566137 MS.gene94866:CDS 40.0%
AGCAATGCTCATGATTACTC+CGG + chr6.1:29566497-29566516 MS.gene94866:CDS 40.0%
AGGAAGGATTTGTGATTCAC+CGG - chr6.1:29566519-29566538 None:intergenic 40.0%
ATAGAGTTCCATTTACCTGC+TGG - chr6.1:29566188-29566207 None:intergenic 40.0%
ATGTGAAAAGACCTTGGATG+TGG - chr6.1:29565413-29565432 None:intergenic 40.0%
ATTCAGTGTAATGGCAAGGA+TGG - chr6.1:29565547-29565566 None:intergenic 40.0%
CAAGCAAGCTTTCAGAAGAT+TGG + chr6.1:29565345-29565364 MS.gene94866:CDS 40.0%
CACAATTCAGTGTAATGGCA+AGG - chr6.1:29565551-29565570 None:intergenic 40.0%
CGGAGTGAAAAAAGTAGTTG+TGG - chr6.1:29564889-29564908 None:intergenic 40.0%
CTTGCCATTACACTGAATTG+TGG + chr6.1:29565549-29565568 MS.gene94866:intron 40.0%
GAAGGGATTTCTGATTGACT+AGG - chr6.1:29566359-29566378 None:intergenic 40.0%
GATTCAATTTCTCTAGCAGC+GGG - chr6.1:29566410-29566429 None:intergenic 40.0%
GATTGATTGAGCTTTCTTGG+GGG - chr6.1:29565101-29565120 None:intergenic 40.0%
GCAGGTAAATGGAACTCTAT+TGG + chr6.1:29566188-29566207 MS.gene94866:CDS 40.0%
GGTAAATGGAACTCTATTGG+TGG + chr6.1:29566191-29566210 MS.gene94866:CDS 40.0%
GTCACCAAACATTTCAAAGC+CGG + chr6.1:29566271-29566290 MS.gene94866:CDS 40.0%
TCACCAAACATTTCAAAGCC+GGG + chr6.1:29566272-29566291 MS.gene94866:CDS 40.0%
TCTGACATATCAGTCATGCT+TGG - chr6.1:29566593-29566612 None:intergenic 40.0%
TGTGAAAAGACCTTGGATGT+GGG - chr6.1:29565412-29565431 None:intergenic 40.0%
! AAAGCTTGCTTGTTCTCACA+TGG - chr6.1:29565337-29565356 None:intergenic 40.0%
! TCATGAGCATTGCTACTCAT+TGG - chr6.1:29566491-29566510 None:intergenic 40.0%
!! AATGTTAGTCAAGTTGGTGG+TGG - chr6.1:29564940-29564959 None:intergenic 40.0%
!! AGGCTTTGATGACTTGTTCT+GGG - chr6.1:29566222-29566241 None:intergenic 40.0%
!! ATCGTCACATTTTGAGAACC+CGG - chr6.1:29566293-29566312 None:intergenic 40.0%
!! CACAACAAAAGTGTCCTCTT+CGG - chr6.1:29566135-29566154 None:intergenic 40.0%
!! TAGGCTTTGATGACTTGTTC+TGG - chr6.1:29566223-29566242 None:intergenic 40.0%
ATGTCGATCATCCAAAGAGC+AGG + chr6.1:29566318-29566337 MS.gene94866:CDS 45.0%
CACAACCATGAAAAACCAGC+AGG + chr6.1:29566170-29566189 MS.gene94866:CDS 45.0%
CGACCTGAAATTTCCAACAG+CGG - chr6.1:29566252-29566271 None:intergenic 45.0%
GACCTGAAATTTCCAACAGC+GGG - chr6.1:29566251-29566270 None:intergenic 45.0%
GCAAGCTTTCAGAAGATTGG+TGG + chr6.1:29565348-29565367 MS.gene94866:CDS 45.0%
GTGAAAAGACCTTGGATGTG+GGG - chr6.1:29565411-29565430 None:intergenic 45.0%
TACCCGCTGTTGGAAATTTC+AGG + chr6.1:29566246-29566265 MS.gene94866:CDS 45.0%
TGGTGGTCGTGGACAAAATA+TGG + chr6.1:29565365-29565384 MS.gene94866:CDS 45.0%
TTCTCTAGCAGCGGGTAAAT+GGG - chr6.1:29566402-29566421 None:intergenic 45.0%
TTTCCAACAGCGGGTAATCT+AGG - chr6.1:29566242-29566261 None:intergenic 45.0%
TTTCTCTAGCAGCGGGTAAA+TGG - chr6.1:29566403-29566422 None:intergenic 45.0%
! TTTCAGAAGATTGGTGGTCG+TGG + chr6.1:29565354-29565373 MS.gene94866:CDS 45.0%
!! CTTTGATGACTTGTTCTGGG+AGG - chr6.1:29566219-29566238 None:intergenic 45.0%
!! GAACCCGGCTTTGAAATGTT+TGG - chr6.1:29566278-29566297 None:intergenic 45.0%
!!! TTAAGATAAATAAATTTTAT+GGG - chr6.1:29565851-29565870 None:intergenic 5.0%
!!! TTTAAGATAAATAAATTTTA+TGG - chr6.1:29565852-29565871 None:intergenic 5.0%
AAGCCTAGATTACCCGCTGT+TGG + chr6.1:29566236-29566255 MS.gene94866:CDS 50.0%
ACTCATTTCAGGGAGGGAGT+TGG + chr6.1:29565921-29565940 MS.gene94866:intron 50.0%
AGCTTTCTTGGGGGTTGTTG+TGG - chr6.1:29565092-29565111 None:intergenic 50.0%
CTAATAGGTCCAGTGGTGGT+CGG - chr6.1:29564909-29564928 None:intergenic 50.0%
CTCATTTCAGGGAGGGAGTT+GGG + chr6.1:29565922-29565941 MS.gene94866:intron 50.0%
! AGTAGTTGTGGGGTTAGCGA+AGG - chr6.1:29564877-29564896 None:intergenic 50.0%
! TTTTTCACTCCGACCACCAC+TGG + chr6.1:29564897-29564916 MS.gene94866:CDS 50.0%
!! TCAAGTTGGTGGTGGTGCTA+GGG - chr6.1:29564932-29564951 None:intergenic 50.0%
AGGGCTAATAGGTCCAGTGG+TGG - chr6.1:29564913-29564932 None:intergenic 55.0%
GCTAGGGCTAATAGGTCCAG+TGG - chr6.1:29564916-29564935 None:intergenic 55.0%
! GTGGTGGTGCTAGGGCTAAT+AGG - chr6.1:29564924-29564943 None:intergenic 55.0%
!! GTCAAGTTGGTGGTGGTGCT+AGG - chr6.1:29564933-29564952 None:intergenic 55.0%
!! GTTAGCGAAGGCGTTGGTAG+TGG - chr6.1:29564865-29564884 None:intergenic 55.0%
! TGTGGGGTTAGCGAAGGCGT+TGG - chr6.1:29564871-29564890 None:intergenic 60.0%
! TTAGCGGGTCCCCACATCCA+AGG + chr6.1:29565399-29565418 MS.gene94866:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 29564858 29566664 29564858 ID=MS.gene94866
chr6.1 mRNA 29564858 29566664 29564858 ID=MS.gene94866.t1;Parent=MS.gene94866
chr6.1 exon 29564858 29565201 29564858 ID=MS.gene94866.t1.exon1;Parent=MS.gene94866.t1
chr6.1 CDS 29564858 29565201 29564858 ID=cds.MS.gene94866.t1;Parent=MS.gene94866.t1
chr6.1 exon 29565304 29565420 29565304 ID=MS.gene94866.t1.exon2;Parent=MS.gene94866.t1
chr6.1 CDS 29565304 29565420 29565304 ID=cds.MS.gene94866.t1;Parent=MS.gene94866.t1
chr6.1 exon 29566067 29566664 29566067 ID=MS.gene94866.t1.exon3;Parent=MS.gene94866.t1
chr6.1 CDS 29566067 29566664 29566067 ID=cds.MS.gene94866.t1;Parent=MS.gene94866.t1
Gene Sequence

>MS.gene94866

ATGACCACTACCAACGCCTTCGCTAACCCCACAACTACTTTTTTCACTCCGACCACCACTGGACCTATTAGCCCTAGCACCACCACCAACTTGACTAACATTATCACTAGTGTTCATGGAACCAGTCAATATCCTACCATTTCAGATTATGCAATACTCATCAAACAACAACAAATTCAACCAAACAATAACAGTCAAGTGTTTGATCATGCATACAACTATTCATTTGTTCCACAACAACCCCCAAGAAAGCTCAATCAATCACAAATTCAATTACCCAACACCAGATCTAGGATTTTACCTAATTCAGATTCACAAAAAGCAAAACATAATTCAAGAATCAGGTAAATCATTTATTATTTTTTTAAATTATTTTTATTATAAATCAATCTTAATGCATTTTAATAATCGAAACATTTAAATGATTTATCTTTCTTATCTTACAGAAAAATTAACAACAAGGTAACTTTGGAATGCCATGTGAGAACAAGCAAGCTTTCAGAAGATTGGTGGTCGTGGACAAAATATGGACAGAAAAACATTAGCGGGTCCCCACATCCAAGGTCTTTTCACATTCAAAAATCTAATTTTTACGTATTCTTGTTTTAGTTCATAATAAAAACAATAATTATGTGATCTAATACAAACATGATACCTTGGGTTTTAAATAGTGGTCTCTAGCATTACCATCCTTGCCATTACACTGAATTGTGGTCTTAATCTAGGTTTTGTTTGTGTTTCAATTTCAAAAACATAATAAATTAGTACTCTATTCTAAAAAATGTGCAACATATTTAAAAAATAAAAAATATGTTATAAAATGAGTGTCATTTTCAATATTCTATGTAATATCAAAATTAATGTATTTTTGGTTTAAGTACAAAATGGATCACTTTCTTCGTCTAATCCATGTAAAAGTTTTGATTGTTACTTTTATTCATTGCAAATATAACAACTTTTTATTTTTTCTACTTGTTGTTTGTTTTTGTTCCCATAAAATTTATTTATCTTAAAATTAGTTCCTGAATATTCATATTAATCCTTGTTCCTGAACAAATATATCACTCATTTCAGGGAGGGAGTTGGGTTGTAACATTTATGTAAAACATGACCAAATAAACAGATATTTTTAGTATATGATTTTAGCTATTAAAGTGATATGATTTAGAGGTGTTTGATATAGAAAACAATCTATGAATGATTTTTCAGGAGCTACTTTAAATGTAGCAGTTCTAAAGATTGTTCAGCAAAAAAACTAGTACAAAAAAGTGACACCGAAGAGGACACTTTTGTTGTGACGTTTGTTGGAGCACACAACCATGAAAAACCAGCAGGTAAATGGAACTCTATTGGTGGGACCTCCCAGAACAAGTCATCAAAGCCTAGATTACCCGCTGTTGGAAATTTCAGGTCGTCACCAAACATTTCAAAGCCGGGTTCTCAAAATGTGACGATGATGCATGTCGATCATCCAAAGAGCAGGAATATTCAAGTTCTCCCTAGTCAATCAGAAATCCCTTCAGAAACTGAACTTATAGAGACCCATTTACCCGCTGCTAGAGAAATTGAATCATCTCCTAATGTTGAAAACTTGTTTTCATATGATGTGACGATGCTACAATCTGATGAGCCAATGAGTAGCAATGCTCATGATTACTCCGGTGAATCACAAATCCTTCCTTCAGAAAACAAACTTATTGGTAACTCTGATGACGATGAAGAAATTATGATACCAAGCATGACTGATATGTCAGAGGATTTCTTATTGGATTTTGACCACCTAAATGATGGTTTTCTATTTCCTTAG
Protein sequence

>MS.gene94866.t1

MTTTNAFANPTTTFFTPTTTGPISPSTTTNLTNIITSVHGTSQYPTISDYAILIKQQQIQPNNNSQVFDHAYNYSFVPQQPPRKLNQSQIQLPNTRSRILPNSDSQKAKHNSRIRKINNKVTLECHVRTSKLSEDWWSWTKYGQKNISGSPHPRSYFKCSSSKDCSAKKLVQKSDTEEDTFVVTFVGAHNHEKPAGKWNSIGGTSQNKSSKPRLPAVGNFRSSPNISKPGSQNVTMMHVDHPKSRNIQVLPSQSEIPSETELIETHLPAAREIESSPNVENLFSYDVTMLQSDEPMSSNAHDYSGESQILPSENKLIGNSDDDEEIMIPSMTDMSEDFLLDFDHLNDGFLFP