AlfalfaGEDB | MS.gene94901

M. sativa cultivar XinJiangDaYe / MS.gene94901

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94901.t1	GAU42190.1	86.3	168	18	4	1	163	105	272	4.20E-69	270.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94901.t1	Q9SND4	85.3	95	14	0	37	131	91	185	2.3e-39	163.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94901.t1	A0A2Z6NZ41	86.3	168	18	4	1	163	105	272	3.1e-69	270.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene94901.t1	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94901.t1	MTR_3g101810	95.758	165	6	1	1	164	107	271	6.11e-114	324
MS.gene94901.t1	MTR_5g017210	64.789	142	47	2	2	142	73	212	4.24e-48	156
MS.gene94901.t1	MTR_8g093500	77.778	90	20	0	38	127	87	176	2.43e-46	152
MS.gene94901.t1	MTR_2g100490	62.366	93	34	1	37	128	261	353	4.68e-27	105
MS.gene94901.t1	MTR_8g015450	55.340	103	42	1	37	135	296	398	6.97e-27	104
MS.gene94901.t1	MTR_7g069590	67.797	59	18	1	71	129	32	89	1.07e-22	88.6
MS.gene94901.t1	MTR_5g079500	62.500	56	21	0	72	127	225	280	4.40e-19	82.4
MS.gene94901.t1	MTR_1g018090	62.963	54	20	0	74	127	283	336	1.55e-18	81.3
MS.gene94901.t1	MTR_1g106460	50.746	67	33	0	61	127	234	300	1.13e-17	78.6
MS.gene94901.t1	MTR_5g005110	61.111	54	21	0	74	127	233	286	1.22e-17	78.6
MS.gene94901.t1	MTR_1g106470	46.914	81	43	0	74	154	205	285	1.48e-17	77.8
MS.gene94901.t1	MTR_3g103030	53.968	63	29	0	65	127	229	291	1.15e-16	75.9
MS.gene94901.t1	MTR_4g131160	56.250	64	27	1	72	134	203	266	4.67e-15	71.6
MS.gene94901.t1	MTR_4g131160	56.250	64	27	1	72	134	206	269	4.89e-15	71.6
MS.gene94901.t1	MTR_1g080890	46.835	79	41	1	58	135	186	264	1.33e-14	70.5
MS.gene94901.t1	MTR_1g106480	45.570	79	36	2	78	154	82	155	2.95e-14	66.6
MS.gene94901.t1	MTR_2g067000	48.000	75	32	1	61	128	146	220	4.14e-13	65.9
MS.gene94901.t1	MTR_4g033295	56.140	57	24	1	75	130	128	184	1.55e-12	62.8
MS.gene94901.t1	MTR_1g060700	55.556	54	24	0	75	128	357	410	2.58e-12	63.9
MS.gene94901.t1	MTR_1g052470	41.573	89	47	2	43	130	280	364	9.99e-12	62.4
MS.gene94901.t1	MTR_2g089070	50.943	53	26	0	75	127	146	198	1.94e-11	60.8
MS.gene94901.t1	MTR_7g110810	49.153	59	26	1	67	125	208	262	2.19e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94901.t1	AT3G50330	77.477	111	24	1	37	146	91	201	4.65e-46	150
MS.gene94901.t1	AT5G67060	70.000	130	27	3	11	131	50	176	5.46e-46	150
MS.gene94901.t1	AT5G67060	72.951	122	22	2	19	131	69	188	7.80e-46	150
MS.gene94901.t1	AT3G21330	52.308	130	59	2	37	163	241	370	2.62e-37	132
MS.gene94901.t1	AT4G00120	69.318	88	27	0	40	127	87	174	2.85e-37	127
MS.gene94901.t1	AT5G09750	76.667	90	21	0	38	127	92	181	1.66e-35	123
MS.gene94901.t1	AT5G01305	67.797	59	19	0	66	124	38	96	3.74e-22	87.8
MS.gene94901.t1	AT5G43175	50.725	69	34	0	59	127	126	194	4.30e-19	80.9
MS.gene94901.t1	AT4G33880	64.815	54	19	0	74	127	275	328	5.34e-19	82.4
MS.gene94901.t1	AT1G27740	52.941	68	32	0	60	127	162	229	1.11e-18	80.5
MS.gene94901.t1	AT5G37800	53.333	75	28	1	60	127	189	263	1.43e-18	80.9
MS.gene94901.t1	AT1G66470	63.636	55	20	0	73	127	203	257	2.03e-18	80.5
MS.gene94901.t1	AT2G14760	62.963	54	20	0	74	127	246	299	6.95e-18	79.3
MS.gene94901.t1	AT2G24260	53.165	79	29	3	63	134	28	105	2.13e-16	74.3
MS.gene94901.t1	AT4G30980	53.333	75	34	1	61	134	126	200	5.47e-16	73.9
MS.gene94901.t1	AT2G24260	53.165	79	29	3	63	134	131	208	6.69e-16	73.9
MS.gene94901.t1	AT4G30980	55.224	67	30	0	61	127	174	240	9.96e-16	73.6
MS.gene94901.t1	AT5G58010	50.000	76	37	1	62	136	96	171	1.41e-14	69.7
MS.gene94901.t1	AT2G14760	50.746	67	20	1	74	127	246	312	1.74e-14	70.1

Find 32 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATTGTAATGTTGATCATTA+TGG	0.211551	8.1:+74626960	MS.gene94901:CDS
ACAGTTGCTGGTTCAGGATT+AGG	0.245927	8.1:+74626906	MS.gene94901:CDS
GATTCTGGGTCTATGTAAAT+TGG	0.284997	8.1:-74626648	None:intergenic
AACAGAACAGTTGCTGGTTC+AGG	0.322536	8.1:+74626900	MS.gene94901:CDS
CAGTTGCTGGTTCAGGATTA+GGG	0.329565	8.1:+74626907	MS.gene94901:CDS
ATTGTAATGTTGATCATTAT+GGG	0.350963	8.1:+74626961	MS.gene94901:CDS
TCACTGCATGAACGTGCTTC+AGG	0.364081	8.1:+74626867	MS.gene94901:CDS
TATGGCTTAGGCAATGAAAC+AGG	0.374640	8.1:-74626933	None:intergenic
CTCGAATTAGAAAACATTAA+CGG	0.391592	8.1:-74626516	None:intergenic
AGCAACTGTTCTGTTATTGT+TGG	0.410949	8.1:-74626892	None:intergenic
GTACCGTAATGGTTTGTATT+CGG	0.415304	8.1:-74626552	None:intergenic
AATGATCAACATTACAATCT+TGG	0.419777	8.1:-74626956	None:intergenic
ACGGTGTTGTTTGTTGTTGT+TGG	0.421243	8.1:-74626497	None:intergenic
TTGACGTTTCTTCTCTTTGG+TGG	0.422338	8.1:-74626678	None:intergenic
AACAATAACAGAACAGTTGC+TGG	0.438471	8.1:+74626894	MS.gene94901:CDS
GCTCCGAATACAAACCATTA+CGG	0.505870	8.1:+74626549	MS.gene94901:CDS
AAACTCGATGGCGGCGATGA+GGG	0.510188	8.1:+74626596	MS.gene94901:CDS
AATAAGAATACTGCAAAGAA+TGG	0.517132	8.1:+74626761	MS.gene94901:CDS
GGTACCAGGTGGAACTAAGA+TGG	0.521420	8.1:+74626782	MS.gene94901:CDS
ATCGAGTTTCTCTTCTCAGA+TGG	0.533422	8.1:-74626582	None:intergenic
TTACAATCTTGGTATGGCTT+AGG	0.541972	8.1:-74626945	None:intergenic
TCAACATTACAATCTTGGTA+TGG	0.551063	8.1:-74626951	None:intergenic
ATGGCAACGCAGTACCGTAA+TGG	0.563985	8.1:-74626563	None:intergenic
GATGGATACTGCATCAATGT+TGG	0.564818	8.1:+74626800	MS.gene94901:CDS
GAAACTCGATGGCGGCGATG+AGG	0.584557	8.1:+74626595	MS.gene94901:CDS
ATCTGAGAAGAGAAACTCGA+TGG	0.628326	8.1:+74626584	MS.gene94901:CDS
TGTCTAGCTGCTATACTCTG+AGG	0.634069	8.1:-74626714	None:intergenic
GTATCCATCTTAGTTCCACC+TGG	0.642640	8.1:-74626786	None:intergenic
ATGGCTTAGGCAATGAAACA+GGG	0.659511	8.1:-74626932	None:intergenic
ATACTGCAAAGAATGGTACC+AGG	0.680757	8.1:+74626768	MS.gene94901:CDS
TGAGAAGAGAAACTCGATGG+CGG	0.685272	8.1:+74626587	MS.gene94901:CDS
CTGCAAAGAATGGTACCAGG+TGG	0.704915	8.1:+74626771	MS.gene94901:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTGTAATGTTGATCATTAT+GGG	+	chr8.1:74626961-74626980	MS.gene94901:CDS	20.0%
!	AATAAGAATACTGCAAAGAA+TGG	+	chr8.1:74626761-74626780	MS.gene94901:CDS	25.0%
!	AATGATCAACATTACAATCT+TGG	-	chr8.1:74626959-74626978	None:intergenic	25.0%
!	CTCGAATTAGAAAACATTAA+CGG	-	chr8.1:74626519-74626538	None:intergenic	25.0%
!	GATTGTAATGTTGATCATTA+TGG	+	chr8.1:74626960-74626979	MS.gene94901:CDS	25.0%
	TCAACATTACAATCTTGGTA+TGG	-	chr8.1:74626954-74626973	None:intergenic	30.0%
!!	ATTTTGACGTTTCTTCTCTT+TGG	-	chr8.1:74626684-74626703	None:intergenic	30.0%
	AACAATAACAGAACAGTTGC+TGG	+	chr8.1:74626894-74626913	MS.gene94901:CDS	35.0%
	AGCAACTGTTCTGTTATTGT+TGG	-	chr8.1:74626895-74626914	None:intergenic	35.0%
	GATTCTGGGTCTATGTAAAT+TGG	-	chr8.1:74626651-74626670	None:intergenic	35.0%
	TTACAATCTTGGTATGGCTT+AGG	-	chr8.1:74626948-74626967	None:intergenic	35.0%
!	GTACCGTAATGGTTTGTATT+CGG	-	chr8.1:74626555-74626574	None:intergenic	35.0%
!!!	TTGGTGGTTTTACTGATTCT+GGG	-	chr8.1:74626665-74626684	None:intergenic	35.0%
!!!	TTTGGTGGTTTTACTGATTC+TGG	-	chr8.1:74626666-74626685	None:intergenic	35.0%
	ATACTGCAAAGAATGGTACC+AGG	+	chr8.1:74626768-74626787	MS.gene94901:CDS	40.0%
	ATCGAGTTTCTCTTCTCAGA+TGG	-	chr8.1:74626585-74626604	None:intergenic	40.0%
	ATCTGAGAAGAGAAACTCGA+TGG	+	chr8.1:74626584-74626603	MS.gene94901:CDS	40.0%
	ATGGCTTAGGCAATGAAACA+GGG	-	chr8.1:74626935-74626954	None:intergenic	40.0%
	GATGGATACTGCATCAATGT+TGG	+	chr8.1:74626800-74626819	MS.gene94901:CDS	40.0%
	GCTCCGAATACAAACCATTA+CGG	+	chr8.1:74626549-74626568	MS.gene94901:CDS	40.0%
	TATGGCTTAGGCAATGAAAC+AGG	-	chr8.1:74626936-74626955	None:intergenic	40.0%
	TTGACGTTTCTTCTCTTTGG+TGG	-	chr8.1:74626681-74626700	None:intergenic	40.0%
!!	ACGGTGTTGTTTGTTGTTGT+TGG	-	chr8.1:74626500-74626519	None:intergenic	40.0%
	GTATCCATCTTAGTTCCACC+TGG	-	chr8.1:74626789-74626808	None:intergenic	45.0%
	TGAGAAGAGAAACTCGATGG+CGG	+	chr8.1:74626587-74626606	MS.gene94901:CDS	45.0%
	TGTCTAGCTGCTATACTCTG+AGG	-	chr8.1:74626717-74626736	None:intergenic	45.0%
!!	AACAGAACAGTTGCTGGTTC+AGG	+	chr8.1:74626900-74626919	MS.gene94901:CDS	45.0%
!!	ACAGTTGCTGGTTCAGGATT+AGG	+	chr8.1:74626906-74626925	MS.gene94901:CDS	45.0%
!!	CAGTTGCTGGTTCAGGATTA+GGG	+	chr8.1:74626907-74626926	MS.gene94901:CDS	45.0%
	ATGGCAACGCAGTACCGTAA+TGG	-	chr8.1:74626566-74626585	None:intergenic	50.0%
	CTGCAAAGAATGGTACCAGG+TGG	+	chr8.1:74626771-74626790	MS.gene94901:CDS	50.0%
	GGTACCAGGTGGAACTAAGA+TGG	+	chr8.1:74626782-74626801	MS.gene94901:CDS	50.0%
	TCACTGCATGAACGTGCTTC+AGG	+	chr8.1:74626867-74626886	MS.gene94901:CDS	50.0%
!	AAACTCGATGGCGGCGATGA+GGG	+	chr8.1:74626596-74626615	MS.gene94901:CDS	55.0%
!	GAAACTCGATGGCGGCGATG+AGG	+	chr8.1:74626595-74626614	MS.gene94901:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	74626492	74626989	74626492	ID=MS.gene94901
chr8.1	mRNA	74626492	74626989	74626492	ID=MS.gene94901.t1;Parent=MS.gene94901
chr8.1	exon	74626492	74626989	74626492	ID=MS.gene94901.t1.exon1;Parent=MS.gene94901.t1
chr8.1	CDS	74626492	74626989	74626492	ID=cds.MS.gene94901.t1;Parent=MS.gene94901.t1

Gene Sequence

>MS.gene94901

ATGTTCCAACAACAACAAACAACACCGTTAATGTTTTCTAATTCGAGCAACGAGATTGCTCCGAATACAAACCATTACGGTACTGCGTTGCCATCTGAGAAGAGAAACTCGATGGCGGCGATGAGGGAGATGATATTCAAAATAGCAGCTATGCAACCAATTTACATAGACCCAGAATCAGTAAAACCACCAAAGAGAAGAAACGTCAAAATTTCAAAAGATCCTCAGAGTATAGCAGCTAGACACAGAAGAGAAAGAATAAGCGAGAAAATAAGAATACTGCAAAGAATGGTACCAGGTGGAACTAAGATGGATACTGCATCAATGTTGGATGAAGCTATTCATTATGTTAAGTTTCTCAAGACACAGCTTAAGTCACTGCATGAACGTGCTTCAGGAGCCAACAATAACAGAACAGTTGCTGGTTCAGGATTAGGGTTCCCTGTTTCATTGCCTAAGCCATACCAAGATTGTAATGTTGATCATTATGGGAGATAA

Protein sequence

>MS.gene94901.t1

MFQQQQTTPLMFSNSSNEIAPNTNHYGTALPSEKRNSMAAMREMIFKIAAMQPIYIDPESVKPPKRRNVKISKDPQSIAARHRRERISEKIRILQRMVPGGTKMDTASMLDEAIHYVKFLKTQLKSLHERASGANNNRTVAGSGLGFPVSLPKPYQDCNVDHYGR