AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95017


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95017.t1 MTR_4g051538 57.258 248 91 5 1 248 1 233 3.86e-84 251
MS.gene95017.t1 MTR_3g104630 54.730 148 60 5 94 238 3 146 8.88e-40 135
MS.gene95017.t1 MTR_3g052920 37.126 167 88 5 1 150 1 167 1.21e-22 93.2
MS.gene95017.t1 MTR_2g030740 37.725 167 87 4 1 150 1 167 1.81e-22 92.8
MS.gene95017.t1 MTR_1g105910 33.036 224 118 7 1 192 1 224 1.81e-22 92.8
MS.gene95017.t1 MTR_1g012570 37.931 174 86 8 1 156 1 170 2.92e-22 92.0
MS.gene95017.t1 MTR_1g077360 30.808 198 111 5 1 187 1 183 3.71e-22 91.7
MS.gene95017.t1 MTR_8g043650 37.086 151 80 3 1 136 1 151 5.45e-22 91.3
MS.gene95017.t1 MTR_1g106070 37.267 161 84 5 1 144 1 161 2.31e-21 88.2
MS.gene95017.t1 MTR_1g108510 33.133 166 98 3 1 156 1 163 2.89e-21 89.4
MS.gene95017.t1 MTR_5g053390 31.163 215 114 8 1 201 1 195 5.48e-21 88.6
MS.gene95017.t1 MTR_3g030770 33.133 166 98 3 1 156 1 163 6.63e-21 88.2
MS.gene95017.t1 MTR_2g085280 28.151 238 137 8 1 206 1 236 8.21e-21 88.2
MS.gene95017.t1 MTR_3g080940 32.727 165 98 3 1 155 1 162 2.99e-20 85.9
MS.gene95017.t1 MTR_2g085250 31.250 160 99 3 1 151 1 158 3.78e-20 86.3
MS.gene95017.t1 MTR_1g105920 36.424 151 81 3 1 136 1 151 5.71e-20 85.9
MS.gene95017.t1 MTR_1g075600 29.545 176 108 4 1 163 1 173 1.35e-19 85.1
MS.gene95017.t1 MTR_8g086290 31.579 171 84 4 4 156 7 162 4.69e-19 82.0
MS.gene95017.t1 MTR_5g045560 34.307 137 80 3 1 127 1 137 4.74e-19 83.6
MS.gene95017.t1 MTR_3g052870 52.778 72 34 0 1 72 1 72 6.80e-19 82.8
MS.gene95017.t1 MTR_1g108580 32.919 161 91 5 1 144 1 161 7.02e-19 82.8
MS.gene95017.t1 MTR_5g055100 27.731 238 134 8 1 204 1 234 1.35e-18 82.4
MS.gene95017.t1 MTR_2g105290 30.247 162 98 4 4 156 7 162 6.34e-18 79.0
MS.gene95017.t1 MTR_2g093190 32.298 161 92 4 1 144 1 161 1.70e-17 79.0
MS.gene95017.t1 MTR_0121s0100 44.156 77 43 0 1 77 1 77 9.61e-17 74.7
MS.gene95017.t1 MTR_3g030780 38.889 108 63 2 1 105 1 108 6.23e-16 72.4
MS.gene95017.t1 MTR_4g094638 50.725 69 34 0 9 77 2 70 6.93e-16 73.6
MS.gene95017.t1 MTR_1g105905 30.270 185 110 6 18 185 1 183 2.10e-15 73.2
MS.gene95017.t1 MTR_0121s0080 30.723 166 98 4 1 149 1 166 8.30e-15 72.0
MS.gene95017.t1 MTR_8g046350 35.345 116 56 3 9 115 2 107 2.08e-14 69.3
MS.gene95017.t1 MTR_4g094632 33.333 144 84 4 9 151 2 134 3.46e-14 68.6
MS.gene95017.t1 MTR_1g047550 36.522 115 61 2 9 115 2 112 4.30e-14 68.9
MS.gene95017.t1 MTR_8g022970 31.288 163 96 3 8 159 17 174 5.60e-14 68.6
MS.gene95017.t1 MTR_8g079502 36.607 112 67 2 9 116 2 113 6.00e-14 68.6
MS.gene95017.t1 MTR_3g067875 50.909 55 27 0 18 72 1 55 6.59e-14 65.5
MS.gene95017.t1 MTR_8g051580 32.143 140 71 4 9 126 2 139 3.26e-13 66.2
MS.gene95017.t1 MTR_1g054265 40.000 70 42 0 9 78 2 71 3.16e-12 63.5
MS.gene95017.t1 MTR_4g131030 34.314 102 58 2 9 101 2 103 3.38e-12 64.3
MS.gene95017.t1 MTR_1g029670 50.000 64 32 0 9 72 2 65 8.60e-12 62.4
MS.gene95017.t1 MTR_1g063160 35.714 98 62 1 9 105 2 99 8.80e-12 62.4
MS.gene95017.t1 MTR_4g102530 49.254 67 31 1 10 76 3 66 3.40e-11 61.2
MS.gene95017.t1 MTR_7g028448 29.921 127 83 4 26 148 29 153 3.49e-11 60.5
MS.gene95017.t1 MTR_4g036915 29.921 127 83 4 26 148 29 153 3.49e-11 60.5
MS.gene95017.t1 MTR_4g127140 27.811 169 103 5 4 156 7 172 6.71e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95017.t1 AT4G36590 35.329 167 91 3 5 156 3 167 5.67e-23 94.4
MS.gene95017.t1 AT1G29962 34.659 176 97 4 1 161 1 173 1.87e-21 88.6
MS.gene95017.t1 AT2G24840 32.642 193 110 5 7 187 61 245 4.25e-21 89.7
MS.gene95017.t1 AT1G28460 34.682 173 98 4 1 161 1 170 1.45e-19 83.6
MS.gene95017.t1 AT1G01530 38.983 118 66 2 5 117 3 119 4.34e-19 84.0
MS.gene95017.t1 AT5G60440 30.660 212 119 6 5 205 3 197 1.19e-18 83.6
MS.gene95017.t1 AT2G34440 31.609 174 102 7 9 174 2 166 4.82e-17 76.6
MS.gene95017.t1 AT1G28450 32.184 174 100 4 1 159 1 171 1.81e-16 75.5
MS.gene95017.t1 AT1G65360 32.759 116 74 1 5 116 3 118 5.58e-16 75.1
MS.gene95017.t1 AT3G04100 41.573 89 49 1 2 90 7 92 8.92e-16 73.9
MS.gene95017.t1 AT3G66656 47.692 65 34 0 9 73 2 66 6.57e-14 68.2
MS.gene95017.t1 AT1G47760 39.759 83 47 1 9 91 2 81 1.04e-12 65.1
MS.gene95017.t1 AT4G11880 49.254 67 32 1 10 76 3 67 6.47e-11 60.1
MS.gene95017.t1 AT4G11880 49.254 67 32 1 10 76 3 67 6.47e-11 60.1
MS.gene95017.t1 AT5G10140 48.214 56 29 0 9 64 2 57 8.95e-11 59.3
MS.gene95017.t1 AT4G11880 49.254 67 32 1 10 76 3 67 9.26e-11 60.5
MS.gene95017.t1 AT4G11880 49.254 67 32 1 10 76 3 67 9.26e-11 60.5
MS.gene95017.t1 AT1G72350 47.541 61 32 0 9 69 43 103 9.41e-11 60.5

Find 45 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCATATCATTCATCTTT+AGG 0.191781 4.4:-40071859 None:intergenic
ACAAATCCTCCTTATGTCTT+TGG 0.244037 4.4:+40071441 MS.gene95017:CDS
AACAACAACGGTTATGAAAT+TGG 0.263911 4.4:+40071468 MS.gene95017:CDS
CAAATCCTCCTTATGTCTTT+GGG 0.278590 4.4:+40071442 MS.gene95017:CDS
ACGGGAAAGTTTGAAGGCTT+TGG 0.328236 4.4:+40071114 MS.gene95017:CDS
GATGTTATTGCTTCTCTTGA+AGG 0.349102 4.4:+40071763 MS.gene95017:CDS
GAAGGTTCTCCATAACAATA+TGG 0.353627 4.4:-40070956 None:intergenic
CTTTGGAAAGTTATGGCTAT+TGG 0.358280 4.4:+40071131 MS.gene95017:CDS
AAAATTGGACACATCTTTAG+TGG 0.370003 4.4:+40071201 MS.gene95017:CDS
CCGCTTGCACGGTCAAGGTT+TGG 0.377489 4.4:-40071805 None:intergenic
TCTGAATTATCAACACTTTG+TGG 0.392582 4.4:+40070893 MS.gene95017:CDS
TTGAAGGCTTTGGAAAGTTA+TGG 0.393387 4.4:+40071124 MS.gene95017:CDS
CTTGACCGTGCAAGCGGAAG+TGG 0.404505 4.4:+40071811 MS.gene95017:CDS
CTTGCTATGGATTGAGAAGA+AGG 0.408194 4.4:-40070974 None:intergenic
GCATGTTGCGGAAGCACAAC+GGG 0.408411 4.4:+40071096 MS.gene95017:CDS
GCAGGAAAACCATATTGTTA+TGG 0.420932 4.4:+40070947 MS.gene95017:CDS
CGTGCAAGCGGAAGTGGAAA+TGG 0.424404 4.4:+40071817 MS.gene95017:CDS
CTGAATTATCAACACTTTGT+GGG 0.424852 4.4:+40070894 MS.gene95017:CDS
TTTAATTCTTCTTCTACTGA+AGG 0.426435 4.4:-40071019 None:intergenic
CTACCTCTTGATGTCCTCTT+CGG 0.429458 4.4:-40070782 None:intergenic
CTTCAAAATTGTTCTTGCTA+TGG 0.431100 4.4:-40070987 None:intergenic
CACTTCCGCTTGCACGGTCA+AGG 0.445314 4.4:-40071810 None:intergenic
ATACTTATTATTTCTCCAGC+AGG 0.450183 4.4:+40070929 MS.gene95017:CDS
CATTTCCACTTCCGCTTGCA+CGG 0.466536 4.4:-40071816 None:intergenic
AGACATAAGGAGGATTTGTC+TGG 0.473975 4.4:-40071437 None:intergenic
TTGTTGACCCCAAAGACATA+AGG 0.475552 4.4:-40071450 None:intergenic
TTAATTCTTCTTCTACTGAA+GGG 0.481788 4.4:-40071018 None:intergenic
AGCACAACGGGAAAGTTTGA+AGG 0.506480 4.4:+40071108 MS.gene95017:CDS
ATTTATGATCCAAAAGGAAA+AGG 0.506756 4.4:+40071736 MS.gene95017:CDS
TCCCACATTTATGATCCAAA+AGG 0.522242 4.4:+40071730 MS.gene95017:CDS
TCTTGCATAGATGAACTGAA+TGG 0.533604 4.4:+40071411 MS.gene95017:CDS
ATAAGTAGTGTCTTAAGCAA+TGG 0.548667 4.4:+40071241 MS.gene95017:CDS
TGAATTATCAACACTTTGTG+GGG 0.569118 4.4:+40070895 MS.gene95017:CDS
CCAAACCTTGACCGTGCAAG+CGG 0.587745 4.4:+40071805 MS.gene95017:CDS
GTCTTTGGGGTCAACAACAA+CGG 0.600048 4.4:+40071456 MS.gene95017:CDS
GGAGGAGAGATTAATCCTCA+TGG 0.604055 4.4:+40071262 MS.gene95017:CDS
TTGACCCCAAAGACATAAGG+AGG 0.605994 4.4:-40071447 None:intergenic
AAATCCTCCTTATGTCTTTG+GGG 0.608431 4.4:+40071443 MS.gene95017:CDS
AGTAGTGTCTTAAGCAATGG+AGG 0.611596 4.4:+40071244 MS.gene95017:CDS
ACAAAGTGTTGATAATTCAG+AGG 0.616841 4.4:-40070891 None:intergenic
AGCATGTTGCGGAAGCACAA+CGG 0.628936 4.4:+40071095 MS.gene95017:CDS
TTTGGATGAAAAGCATGTTG+CGG 0.629913 4.4:+40071084 MS.gene95017:CDS
TGGTTAAGATGGCTCCGAAG+AGG 0.660173 4.4:+40070768 None:intergenic
GCTCCGAAGAGGACATCAAG+AGG 0.673948 4.4:+40070779 MS.gene95017:CDS
GAAGGTAGAAATAAAACACA+AGG 0.705496 4.4:+40071781 MS.gene95017:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTATTTATGAAAAAG+TGG + chr4.4:40071365-40071384 MS.gene95017:intron 15.0%
!! GAAATTATACAATATGTGTT+AGG - chr4.4:40071690-40071709 None:intergenic 20.0%
!! TTATGACCAGTTAAAAAAAT+TGG + chr4.4:40071186-40071205 MS.gene95017:CDS 20.0%
!!! AATGTCTTCATATCTTTTTT+TGG - chr4.4:40071642-40071661 None:intergenic 20.0%
!!! ATGTCTTCATATCTTTTTTT+GGG - chr4.4:40071641-40071660 None:intergenic 20.0%
! ACTCTTGTGTTATATGAATT+TGG - chr4.4:40071514-40071533 None:intergenic 25.0%
! AGTATAGATATTTGATGTGT+TGG - chr4.4:40071556-40071575 None:intergenic 25.0%
! ATTTATGATCCAAAAGGAAA+AGG + chr4.4:40071736-40071755 MS.gene95017:CDS 25.0%
! GCATTAAAAATGGTTAAAGT+TGG - chr4.4:40071580-40071599 None:intergenic 25.0%
! TCTCATAAGTGCATTAAAAA+TGG - chr4.4:40071590-40071609 None:intergenic 25.0%
! TTAATTCTTCTTCTACTGAA+GGG - chr4.4:40071021-40071040 None:intergenic 25.0%
! TTTAATTCTTCTTCTACTGA+AGG - chr4.4:40071022-40071041 None:intergenic 25.0%
!!! ATGTGTCCAATTTTTTTAAC+TGG - chr4.4:40071195-40071214 None:intergenic 25.0%
AAAATTGGACACATCTTTAG+TGG + chr4.4:40071201-40071220 MS.gene95017:CDS 30.0%
AACAACAACGGTTATGAAAT+TGG + chr4.4:40071468-40071487 MS.gene95017:CDS 30.0%
ATAAGTAGTGTCTTAAGCAA+TGG + chr4.4:40071241-40071260 MS.gene95017:CDS 30.0%
CTGAATTATCAACACTTTGT+GGG + chr4.4:40070894-40070913 MS.gene95017:CDS 30.0%
CTTCAAAATTGTTCTTGCTA+TGG - chr4.4:40070990-40071009 None:intergenic 30.0%
GAAGGTAGAAATAAAACACA+AGG + chr4.4:40071781-40071800 MS.gene95017:CDS 30.0%
TCTGAATTATCAACACTTTG+TGG + chr4.4:40070893-40070912 MS.gene95017:CDS 30.0%
TGAATTATCAACACTTTGTG+GGG + chr4.4:40070895-40070914 MS.gene95017:CDS 30.0%
TGTACATCTTCTAATCAACT+TGG + chr4.4:40071289-40071308 MS.gene95017:intron 30.0%
! ATACTTATTATTTCTCCAGC+AGG + chr4.4:40070929-40070948 MS.gene95017:CDS 30.0%
! CACAAAAAATGATGAGCTTT+TGG + chr4.4:40071066-40071085 MS.gene95017:CDS 30.0%
! TCCTTTTGGATCATAAATGT+GGG - chr4.4:40071734-40071753 None:intergenic 30.0%
! TTCCTTTTGGATCATAAATG+TGG - chr4.4:40071735-40071754 None:intergenic 30.0%
!! ACAAAGTGTTGATAATTCAG+AGG - chr4.4:40070894-40070913 None:intergenic 30.0%
AAATCCTCCTTATGTCTTTG+GGG + chr4.4:40071443-40071462 MS.gene95017:CDS 35.0%
ACAAATCCTCCTTATGTCTT+TGG + chr4.4:40071441-40071460 MS.gene95017:CDS 35.0%
CAAATCCTCCTTATGTCTTT+GGG + chr4.4:40071442-40071461 MS.gene95017:CDS 35.0%
GAAGGTTCTCCATAACAATA+TGG - chr4.4:40070959-40070978 None:intergenic 35.0%
GATGTTATTGCTTCTCTTGA+AGG + chr4.4:40071763-40071782 MS.gene95017:CDS 35.0%
GCAGGAAAACCATATTGTTA+TGG + chr4.4:40070947-40070966 MS.gene95017:CDS 35.0%
TCCCACATTTATGATCCAAA+AGG + chr4.4:40071730-40071749 MS.gene95017:CDS 35.0%
TCTTGCATAGATGAACTGAA+TGG + chr4.4:40071411-40071430 MS.gene95017:CDS 35.0%
TTTCTTGTCATTGAAGCTCA+TGG + chr4.4:40071706-40071725 MS.gene95017:intron 35.0%
! ACATCATTGCCTTTTCCTTT+TGG - chr4.4:40071748-40071767 None:intergenic 35.0%
! CTTTGGAAAGTTATGGCTAT+TGG + chr4.4:40071131-40071150 MS.gene95017:CDS 35.0%
! GCATTTTCAAAGAGAAAGTC+TGG + chr4.4:40070854-40070873 MS.gene95017:CDS 35.0%
! TAACAATATGGTTTTCCTGC+TGG - chr4.4:40070947-40070966 None:intergenic 35.0%
! TTTGGATGAAAAGCATGTTG+CGG + chr4.4:40071084-40071103 MS.gene95017:CDS 35.0%
!! TTGAAGGCTTTGGAAAGTTA+TGG + chr4.4:40071124-40071143 MS.gene95017:CDS 35.0%
AGACATAAGGAGGATTTGTC+TGG - chr4.4:40071440-40071459 None:intergenic 40.0%
AGTAGTGTCTTAAGCAATGG+AGG + chr4.4:40071244-40071263 MS.gene95017:CDS 40.0%
TTGTTGACCCCAAAGACATA+AGG - chr4.4:40071453-40071472 None:intergenic 40.0%
! CTTGCTATGGATTGAGAAGA+AGG - chr4.4:40070977-40070996 None:intergenic 40.0%
ACGGGAAAGTTTGAAGGCTT+TGG + chr4.4:40071114-40071133 MS.gene95017:CDS 45.0%
AGCACAACGGGAAAGTTTGA+AGG + chr4.4:40071108-40071127 MS.gene95017:CDS 45.0%
CTACCTCTTGATGTCCTCTT+CGG - chr4.4:40070785-40070804 None:intergenic 45.0%
GAAGATGTACACGTACCATG+AGG - chr4.4:40071280-40071299 None:intergenic 45.0%
GGAGGAGAGATTAATCCTCA+TGG + chr4.4:40071262-40071281 MS.gene95017:CDS 45.0%
TTGACCCCAAAGACATAAGG+AGG - chr4.4:40071450-40071469 None:intergenic 45.0%
!! GTCTTTGGGGTCAACAACAA+CGG + chr4.4:40071456-40071475 MS.gene95017:CDS 45.0%
CATTTCCACTTCCGCTTGCA+CGG - chr4.4:40071819-40071838 None:intergenic 50.0%
! AGCATGTTGCGGAAGCACAA+CGG + chr4.4:40071095-40071114 MS.gene95017:CDS 50.0%
CCAAACCTTGACCGTGCAAG+CGG + chr4.4:40071805-40071824 MS.gene95017:CDS 55.0%
CGTGCAAGCGGAAGTGGAAA+TGG + chr4.4:40071817-40071836 MS.gene95017:CDS 55.0%
GCTCCGAAGAGGACATCAAG+AGG + chr4.4:40070779-40070798 MS.gene95017:CDS 55.0%
! GCATGTTGCGGAAGCACAAC+GGG + chr4.4:40071096-40071115 MS.gene95017:CDS 55.0%
CACTTCCGCTTGCACGGTCA+AGG - chr4.4:40071813-40071832 None:intergenic 60.0%
CCGCTTGCACGGTCAAGGTT+TGG - chr4.4:40071808-40071827 None:intergenic 60.0%
CTTGACCGTGCAAGCGGAAG+TGG + chr4.4:40071811-40071830 MS.gene95017:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 40070776 40071873 40070776 ID=MS.gene95017
chr4.4 mRNA 40070776 40071873 40070776 ID=MS.gene95017.t1;Parent=MS.gene95017
chr4.4 exon 40070776 40071283 40070776 ID=MS.gene95017.t1.exon1;Parent=MS.gene95017.t1
chr4.4 CDS 40070776 40071283 40070776 ID=cds.MS.gene95017.t1;Parent=MS.gene95017.t1
chr4.4 exon 40071406 40071495 40071406 ID=MS.gene95017.t1.exon2;Parent=MS.gene95017.t1
chr4.4 CDS 40071406 40071495 40071406 ID=cds.MS.gene95017.t1;Parent=MS.gene95017.t1
chr4.4 exon 40071722 40071873 40071722 ID=MS.gene95017.t1.exon3;Parent=MS.gene95017.t1
chr4.4 CDS 40071722 40071873 40071722 ID=cds.MS.gene95017.t1;Parent=MS.gene95017.t1
Gene Sequence

>MS.gene95017

ATGGCTCCGAAGAGGACATCAAGAGGTAGACAAAAAAGAGAAATGAAGAAGATTGAGAGAGAAAGCGATCTTTTAGTAGCATTTTCAAAGAGAAAGTCTGGCATTTACAACAAAGCCTCTGAATTATCAACACTTTGTGGGGCTTTAGTTGATATACTTATTATTTCTCCAGCAGGAAAACCATATTGTTATGGAGAACCTTCTTCTCAATCCATAGCAAGAACAATTTTGAAGAATGAAAACCCTTCAGTAGAAGAAGAATTAAAGAAACTAAAGATTAACAAAGTTAACACAAAAAATGATGAGCTTTTGGATGAAAAGCATGTTGCGGAAGCACAACGGGAAAGTTTGAAGGCTTTGGAAAGTTATGGCTATTGGTCGATCAATGAGAAAGATGTTGAAAGCTTTGATTATGACCAGTTAAAAAAATTGGACACATCTTTAGTGGACATTGCAAATAAATTGATAAGTAGTGTCTTAAGCAATGGAGGAGAGATTAATCCTCATGGTACGTGTACATCTTCTAATCAACTTGGTCTTATATTTGATATATTAATGATGTCAAATGATTTCATTTCATGTTTTCTGTCTAATTTATTTATGAAAAAGTGGTTATAATTTTATTTGCAGCAATTTCTTGCATAGATGAACTGAATGGAGTCCAGACAAATCCTCCTTATGTCTTTGGGGTCAACAACAACGGTTATGAAATTGGTGCAAGTAAGAAATCTCATTCCAAATTCATATAACACAAGAGTAATACATATTACTCGCACTCCAACACATCAAATATCTATACTGCCAACTTTAACCATTTTTAATGCACTTATGAGATAAATCTTGTTTATTTTTTCATTTTGATCCCAAAAAAAGATATGAAGACATTAAATTTTAATATTACATGCGATAAACCTAACACATATTGTATAATTTCTTGTCATTGAAGCTCATGGATCCCACATTTATGATCCAAAAGGAAAAGGCAATGATGTTATTGCTTCTCTTGAAGGTAGAAATAAAACACAAGGACCAAACCTTGACCGTGCAAGCGGAAGTGGAAATGGAAGTGATGTTGATCATTCTCCTAAAGATGAATGA

Protein sequence

>MS.gene95017.t1

MAPKRTSRGRQKREMKKIERESDLLVAFSKRKSGIYNKASELSTLCGALVDILIISPAGKPYCYGEPSSQSIARTILKNENPSVEEELKKLKINKVNTKNDELLDEKHVAEAQRESLKALESYGYWSINEKDVESFDYDQLKKLDTSLVDIANKLISSVLSNGGEINPHAISCIDELNGVQTNPPYVFGVNNNGYEIGATHGSHIYDPKGKGNDVIASLEGRNKTQGPNLDRASGSGNGSDVDHSPKDE