AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95257

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95257.t1 MTR_4g121110 82.796 93 2 3 52 131 27 118 1.70e-44 142
MS.gene95257.t1 MTR_7g112330 45.977 87 33 4 54 131 21 102 1.67e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95257.t1 AT5G49300 62.667 75 20 4 54 124 35 105 1.13e-19 79.3
MS.gene95257.t1 AT3G06740 54.321 81 31 3 54 131 40 117 2.54e-19 78.6
MS.gene95257.t1 AT4G16141 90.625 32 2 1 54 85 36 66 7.21e-14 65.5
MS.gene95257.t1 AT3G16870 58.929 56 14 2 54 101 41 95 6.40e-13 62.8
MS.gene95257.t1 AT4G36620 54.902 51 22 1 55 105 75 124 2.29e-11 58.9

Find 37 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAGAGATTATTGAATTT+AGG 0.186034 8.4:+60545952 MS.gene95257:CDS
AAGAAATTGATAGAGTTAAA+TGG 0.194175 8.4:-60543238 None:intergenic
AGCAGAGATTATTGAATTTA+GGG 0.227823 8.4:+60545953 MS.gene95257:CDS
TTGATAGAGTTAAATGGGTT+TGG 0.293730 8.4:-60543232 None:intergenic
ACCACGCCATAGAGGGGTTT+TGG 0.316833 8.4:-60545701 None:intergenic
TCTGATGAAACGGCGGCGTT+AGG 0.333300 8.4:-60545633 None:intergenic
TGATAGAGTTAAATGGGTTT+GGG 0.354928 8.4:-60543231 None:intergenic
AGAAATTGATAGAGTTAAAT+GGG 0.358975 8.4:-60543237 None:intergenic
GAACTTCCAAAACCCCTCTA+TGG 0.380563 8.4:+60545695 MS.gene95257:CDS
CTGATGAAACGGCGGCGTTA+GGG 0.382761 8.4:-60545632 None:intergenic
AACGAAGAAGGAACAAGAAA+GGG 0.398579 8.4:+60545871 MS.gene95257:CDS
TAAATTACCTTTGGTCCTGC+AGG 0.425818 8.4:-60545730 None:intergenic
AGTAACAGTGGCGGTGGCGG+TGG 0.436374 8.4:+60545901 MS.gene95257:CDS
CAACGAAGAAGGAACAAGAA+AGG 0.448446 8.4:+60545870 MS.gene95257:CDS
TGGCGTGGTGGTCCTCCTGC+AGG 0.457256 8.4:+60545715 MS.gene95257:CDS
AGTCACTGTGCAATGCTTGC+GGG 0.503081 8.4:+60545833 MS.gene95257:intron
GGTGGCGGTGGAAGCAAAGT+TGG 0.505807 8.4:+60545913 MS.gene95257:CDS
GAGGACCACCACGCCATAGA+GGG 0.522026 8.4:-60545708 None:intergenic
AGCAAAGGAAACAACGAAGA+AGG 0.525059 8.4:+60545859 MS.gene95257:CDS
ATTGTTGTTATCTGATGAAA+CGG 0.531824 8.4:-60545643 None:intergenic
AAGAGTAACAGTGGCGGTGG+CGG 0.533652 8.4:+60545898 MS.gene95257:CDS
GGAGGACCACCACGCCATAG+AGG 0.541418 8.4:-60545709 None:intergenic
CTTCATCCCTCCTCAAGTCA+AGG 0.554954 8.4:-60543356 None:intergenic
TGGTAGACCTTGACTTGAGG+AGG 0.561236 8.4:+60543349 MS.gene95257:CDS
CAGTCACTGTGCAATGCTTG+CGG 0.579698 8.4:+60545832 MS.gene95257:intron
TGGTCCTCCTGCAGGACCAA+AGG 0.595556 8.4:+60545723 MS.gene95257:CDS
ATTACCTTTGGTCCTGCAGG+AGG 0.596413 8.4:-60545727 None:intergenic
CTAATTATTAGGGAAAGTTG+TGG 0.613984 8.4:+60543329 MS.gene95257:intron
AAGAAAACATGTGCTGATTG+CGG 0.615546 8.4:+60545673 MS.gene95257:CDS
ACGAAGAAGGAACAAGAAAG+GGG 0.621165 8.4:+60545872 MS.gene95257:CDS
TTGTGGTAGACCTTGACTTG+AGG 0.637118 8.4:+60543346 MS.gene95257:CDS
TCCAAAACCCCTCTATGGCG+TGG 0.637585 8.4:+60545700 MS.gene95257:CDS
AATGCTTGCGGGATAAGCAA+AGG 0.651532 8.4:+60545844 MS.gene95257:CDS
AGGACCACCACGCCATAGAG+GGG 0.652889 8.4:-60545707 None:intergenic
AAAACCCCTCTATGGCGTGG+TGG 0.672135 8.4:+60545703 MS.gene95257:CDS
GGTAGACCTTGACTTGAGGA+GGG 0.676317 8.4:+60543350 MS.gene95257:CDS
GTTGTTATCTGATGAAACGG+CGG 0.695776 8.4:-60545640 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACCAAAATATATTTTTTA+TGG + chr8.4:60543760-60543779 MS.gene95257:intron 10.0%
!!! ATAAATGAAAGTATAAATAT+GGG - chr8.4:60543532-60543551 None:intergenic 10.0%
!!! TAGTAAGATAATATTTTTTT+AGG + chr8.4:60543478-60543497 MS.gene95257:intron 10.0%
!!! TATAAATGAAAGTATAAATA+TGG - chr8.4:60543533-60543552 None:intergenic 10.0%
!!! TTTTATTTCTTCTTTATATT+TGG + chr8.4:60545086-60545105 MS.gene95257:intron 10.0%
!! AAAAAAAAAAACTGATCATT+TGG + chr8.4:60543916-60543935 MS.gene95257:intron 15.0%
!! AAGTTATTAATGTTACAATT+TGG - chr8.4:60544515-60544534 None:intergenic 15.0%
!! ATTAGATATTCCTAATTATT+AGG + chr8.4:60543318-60543337 MS.gene95257:intron 15.0%
!! ATTCTCATTAATTATTAAAG+TGG + chr8.4:60543848-60543867 MS.gene95257:intron 15.0%
!! ATTGTAACATTAATAACTTA+TGG + chr8.4:60544516-60544535 MS.gene95257:intron 15.0%
!! GAAACAAAAAAATAAGTTAA+GGG - chr8.4:60544264-60544283 None:intergenic 15.0%
!! TAAAAATTATCAACTTAAAG+AGG - chr8.4:60543298-60543317 None:intergenic 15.0%
!! TAACCAAATAAAATATTGAT+GGG - chr8.4:60544827-60544846 None:intergenic 15.0%
!! TAATCTATAATAAATATCAC+CGG + chr8.4:60545234-60545253 MS.gene95257:intron 15.0%
!! TAATCTTGATTATTTATCAT+AGG + chr8.4:60543665-60543684 MS.gene95257:intron 15.0%
!! TTAGATATTCCTAATTATTA+GGG + chr8.4:60543319-60543338 MS.gene95257:intron 15.0%
!! TTGTAACATTAATAACTTAT+GGG + chr8.4:60544517-60544536 MS.gene95257:intron 15.0%
!!! AAGAATTTTTTTTGAATAAC+TGG + chr8.4:60544780-60544799 MS.gene95257:intron 15.0%
!!! ATTAATGAGAATTTTTCTTT+AGG - chr8.4:60543840-60543859 None:intergenic 15.0%
!!! TATACTTTCATTTATACTTT+TGG + chr8.4:60543537-60543556 MS.gene95257:intron 15.0%
!!! TCAAAAAAAATTCTTCTTTT+GGG - chr8.4:60544775-60544794 None:intergenic 15.0%
!!! TTCAAAAAAAATTCTTCTTT+TGG - chr8.4:60544776-60544795 None:intergenic 15.0%
!! AAAACCAAAAAGTAAGTTAA+GGG - chr8.4:60544204-60544223 None:intergenic 20.0%
!! AAACATGTCAAATTATTAAG+AGG + chr8.4:60543715-60543734 MS.gene95257:intron 20.0%
!! AACCAAATAAAATATTGATG+GGG - chr8.4:60544826-60544845 None:intergenic 20.0%
!! AAGAAATTGATAGAGTTAAA+TGG - chr8.4:60543241-60543260 None:intergenic 20.0%
!! AAGTTATTAACGTTACAATT+TGG + chr8.4:60544177-60544196 MS.gene95257:intron 20.0%
!! AGAAATTGATAGAGTTAAAT+GGG - chr8.4:60543240-60543259 None:intergenic 20.0%
!! ATACTTCAAAACAATATGAA+AGG - chr8.4:60543574-60543593 None:intergenic 20.0%
!! ATCATATTATTCTCTCAAAA+AGG + chr8.4:60544029-60544048 MS.gene95257:intron 20.0%
!! ATTGTAACGTTAATAACTTA+AGG - chr8.4:60544176-60544195 None:intergenic 20.0%
!! CGAAACAAAAAAATAAGTTA+AGG - chr8.4:60544265-60544284 None:intergenic 20.0%
!! CGAAATCAAAAAATAAGTTA+AGG + chr8.4:60544397-60544416 MS.gene95257:intron 20.0%
!! CTTAATAATTTGACATGTTT+CGG - chr8.4:60543715-60543734 None:intergenic 20.0%
!! GAAACCAAAAAATAAGTTAA+GGG + chr8.4:60544428-60544447 MS.gene95257:intron 20.0%
!! GAAACCAAAAAATAAGTTAA+GGG - chr8.4:60544447-60544428 None:intergenic 20.0%
!! GAGAATAATATGATATGATA+TGG - chr8.4:60544023-60544042 None:intergenic 20.0%
!! GTAACCAAATAAAATATTGA+TGG - chr8.4:60544828-60544847 None:intergenic 20.0%
!! GTGATTAATTAAATTACCTT+TGG - chr8.4:60545742-60545761 None:intergenic 20.0%
!! TAAAAATACTCCTAATAACT+TGG - chr8.4:60543787-60543806 None:intergenic 20.0%
!! TTGAACTATTAAATCATGAA+TGG - chr8.4:60545549-60545568 None:intergenic 20.0%
!! TTGTAACGTTAATAACTTAA+GGG - chr8.4:60544175-60544194 None:intergenic 20.0%
!! TTTAGGCTTAATTAATGATT+TGG - chr8.4:60544575-60544594 None:intergenic 20.0%
!! TTTATAAAAGTTGAGCTTTA+TGG - chr8.4:60544951-60544970 None:intergenic 20.0%
!!! AAACAATTTTGTTGGTTTAA+AGG + chr8.4:60544851-60544870 MS.gene95257:intron 20.0%
!!! AAACCATATCATATTTTTTC+TGG + chr8.4:60543986-60544005 MS.gene95257:intron 20.0%
!!! AACGAAACTTTTAATAGTTA+AGG + chr8.4:60544456-60544475 MS.gene95257:intron 20.0%
!!! AACGAAACTTTTAATAGTTA+AGG - chr8.4:60544475-60544456 None:intergenic 20.0%
!!! AACTATTAAAAGTTTCGTTT+TGG + chr8.4:60544237-60544256 MS.gene95257:intron 20.0%
!!! AACTATTAAAAGTTTCGTTT+TGG - chr8.4:60544256-60544237 None:intergenic 20.0%
!!! ACGAAACTTTTAATAGTTAA+GGG + chr8.4:60544457-60544476 MS.gene95257:intron 20.0%
!!! ACGAAACTTTTAATAGTTAA+GGG - chr8.4:60544476-60544457 None:intergenic 20.0%
!!! CAAAAAAAATTCTTCTTTTG+GGG - chr8.4:60544774-60544793 None:intergenic 20.0%
!!! CTTATTTTTTGATTTCGTTT+TGG - chr8.4:60544394-60544413 None:intergenic 20.0%
!!! CTTATTTTTTTGTTTCGTTT+TGG + chr8.4:60544268-60544287 MS.gene95257:intron 20.0%
!!! GGAAAACTATTTTATGAATA+GGG - chr8.4:60543600-60543619 None:intergenic 20.0%
!!! GGACCATAAAAAATATATTT+TGG - chr8.4:60543766-60543785 None:intergenic 20.0%
!!! GTTAATTATAATGTTGTTTC+TGG + chr8.4:60543264-60543283 MS.gene95257:intron 20.0%
!!! TCGGATTTTATTAAACTAAT+AGG - chr8.4:60543696-60543715 None:intergenic 20.0%
!!! TGGAAAACTATTTTATGAAT+AGG - chr8.4:60543601-60543620 None:intergenic 20.0%
!!! TGTTAGTTAAATTTCCTTTT+TGG - chr8.4:60545413-60545432 None:intergenic 20.0%
!!! TTTCATGGTATTAATTTTGA+TGG - chr8.4:60544687-60544706 None:intergenic 20.0%
!!! TTTCGAGTTAAAATTTTCTT+AGG - chr8.4:60543899-60543918 None:intergenic 20.0%
!!! TTTGTTCATTTTTCATTTTC+AGG + chr8.4:60545587-60545606 MS.gene95257:intron 20.0%
!!! TTTTTTTGAATAACTGGTAT+AGG + chr8.4:60544786-60544805 MS.gene95257:intron 20.0%
! AAGCAGAGATTATTGAATTT+AGG + chr8.4:60545952-60545971 MS.gene95257:CDS 25.0%
! AAGTATAAATATGGGAAACA+AGG - chr8.4:60543524-60543543 None:intergenic 25.0%
! AATTGTGTAATATTCTCGTT+TGG + chr8.4:60543640-60543659 MS.gene95257:intron 25.0%
! AGCAGAGATTATTGAATTTA+GGG + chr8.4:60545953-60545972 MS.gene95257:CDS 25.0%
! CAAAACCAAAAAGTAAGTTA+AGG - chr8.4:60544205-60544224 None:intergenic 25.0%
! CGAAACCAAAAAATAAGTTA+AGG + chr8.4:60544427-60544446 MS.gene95257:intron 25.0%
! CGAAACCAAAAAATAAGTTA+AGG - chr8.4:60544446-60544427 None:intergenic 25.0%
! GAAACCAAAAAGTAACTTAA+GGG + chr8.4:60544488-60544507 MS.gene95257:intron 25.0%
! GATGAATGTATTAGAGTTAA+GGG - chr8.4:60544326-60544345 None:intergenic 25.0%
! GTAACCAACAAATAACTTAA+GGG + chr8.4:60544548-60544567 MS.gene95257:intron 25.0%
! TGACCAGAAAAAATATGATA+TGG - chr8.4:60543992-60544011 None:intergenic 25.0%
! TGTAACCAACAAATAACTTA+AGG + chr8.4:60544547-60544566 MS.gene95257:intron 25.0%
! TGTCAAATTATTAAGAGGTT+TGG + chr8.4:60543720-60543739 MS.gene95257:intron 25.0%
! TTAATATTCTTGTGAATGAG+TGG - chr8.4:60543418-60543437 None:intergenic 25.0%
!! ATTGTTGTTATCTGATGAAA+CGG - chr8.4:60545646-60545665 None:intergenic 25.0%
!! GTTACTTCAAACAATTTTGT+TGG + chr8.4:60544843-60544862 MS.gene95257:intron 25.0%
!! TACCCCATCAATATTTTATT+TGG + chr8.4:60544821-60544840 MS.gene95257:intron 25.0%
!! TCTCTCGTTTATTAGATTTT+AGG - chr8.4:60544592-60544611 None:intergenic 25.0%
!! TTTTTATGGTCCAAGTTATT+AGG + chr8.4:60543774-60543793 MS.gene95257:intron 25.0%
!!! CTTACTTTTTGGTTTTGTTT+TGG + chr8.4:60544208-60544227 MS.gene95257:intron 25.0%
!!! CTTATTTTTTGGTTTCGTTT+TGG + chr8.4:60544148-60544167 MS.gene95257:intron 25.0%
!!! CTTATTTTTTGGTTTCGTTT+TGG - chr8.4:60544167-60544148 None:intergenic 25.0%
!!! GTTATTTGTTGGTTACATTT+TGG - chr8.4:60544544-60544563 None:intergenic 25.0%
!!! TGGTACCTTAACTTATTTTT+TGG + chr8.4:60544137-60544156 MS.gene95257:intron 25.0%
AAACGAGAGAAAATCTTACA+AGG + chr8.4:60544602-60544621 MS.gene95257:intron 30.0%
AACACACAATAAAGACATGA+GGG + chr8.4:60545429-60545448 MS.gene95257:intron 30.0%
AAGGTATGAAACGTATATGT+TGG - chr8.4:60544644-60544663 None:intergenic 30.0%
AGATGATGAAAGATGTCTAT+TGG + chr8.4:60545283-60545302 MS.gene95257:intron 30.0%
AGTCACTAAAACGATGAATT+TGG - chr8.4:60544058-60544077 None:intergenic 30.0%
ATACATTCATCCTAACGTAA+AGG + chr8.4:60544335-60544354 MS.gene95257:intron 30.0%
ATGAAATGATCACAGTGATA+AGG + chr8.4:60545472-60545491 MS.gene95257:intron 30.0%
CACAACTTTCCCTAATAATT+AGG - chr8.4:60543331-60543350 None:intergenic 30.0%
CGAAACCAAAAAGTAACTTA+AGG + chr8.4:60544487-60544506 MS.gene95257:intron 30.0%
CTAATTATTAGGGAAAGTTG+TGG + chr8.4:60543329-60543348 MS.gene95257:intron 30.0%
CTTGATAAATTGCACCAAAA+AGG + chr8.4:60545396-60545415 MS.gene95257:intron 30.0%
GAATATTACACAATTCGCTA+AGG - chr8.4:60543635-60543654 None:intergenic 30.0%
GGATGAATGTATTAGAGTTA+AGG - chr8.4:60544327-60544346 None:intergenic 30.0%
GTATTCTTAGATACTTTCTC+CGG - chr8.4:60545256-60545275 None:intergenic 30.0%
TAACACACAATAAAGACATG+AGG + chr8.4:60545428-60545447 MS.gene95257:intron 30.0%
! ACTAGGCTTAATTACTCTTT+TGG + chr8.4:60544117-60544136 MS.gene95257:intron 30.0%
! TGATAGAGTTAAATGGGTTT+GGG - chr8.4:60543234-60543253 None:intergenic 30.0%
! TTGATAGAGTTAAATGGGTT+TGG - chr8.4:60543235-60543254 None:intergenic 30.0%
!! TGCAATTTATCAAGTTGAGT+AGG - chr8.4:60545390-60545409 None:intergenic 30.0%
!!! GTTACTTTTTGGTTTCGTTT+TGG - chr8.4:60544484-60544503 None:intergenic 30.0%
!!! TGGTCCCTTAACTTATTTTT+TGG + chr8.4:60544257-60544276 MS.gene95257:intron 30.0%
!!! TGGTCCCTTAACTTATTTTT+TGG - chr8.4:60544276-60544257 None:intergenic 30.0%
!!! TTTCATTTTCAGGTATCAGA+AGG + chr8.4:60545597-60545616 MS.gene95257:intron 30.0%
AACGAAGAAGGAACAAGAAA+GGG + chr8.4:60545871-60545890 MS.gene95257:CDS 35.0%
ATGGTTTGATCCATTTGTCA+TGG - chr8.4:60543973-60543992 None:intergenic 35.0%
ATTCTTGTGAATGAGTGGAA+AGG - chr8.4:60543413-60543432 None:intergenic 35.0%
TGGTCCCTTAAGTTATTTGT+TGG - chr8.4:60544555-60544574 None:intergenic 35.0%
TTGAAGTGAAGTGTGTGATT+AGG - chr8.4:60545526-60545545 None:intergenic 35.0%
! CAATCAGCACATGTTTTCTT+TGG - chr8.4:60545673-60545692 None:intergenic 35.0%
!! AAGAAAACATGTGCTGATTG+CGG + chr8.4:60545673-60545692 MS.gene95257:CDS 35.0%
!! GGTTCAGGTTTGATTTTTCA+TGG - chr8.4:60544702-60544721 None:intergenic 35.0%
!! TGGTCCCTTAACTTACTTTT+TGG + chr8.4:60544197-60544216 MS.gene95257:intron 35.0%
!!! AAGCTCGCACAATTTTTTAG+AGG - chr8.4:60545115-60545134 None:intergenic 35.0%
!!! AGCTCGCACAATTTTTTAGA+GGG - chr8.4:60545114-60545133 None:intergenic 35.0%
!!! TGGTCCCTTAAGTTACTTTT+TGG - chr8.4:60544495-60544514 None:intergenic 35.0%
AAGGGGAAAAAGAGTAACAG+TGG + chr8.4:60545889-60545908 MS.gene95257:CDS 40.0%
ACGAAGAAGGAACAAGAAAG+GGG + chr8.4:60545872-60545891 MS.gene95257:CDS 40.0%
AGCAAAGGAAACAACGAAGA+AGG + chr8.4:60545859-60545878 MS.gene95257:CDS 40.0%
CAACGAAGAAGGAACAAGAA+AGG + chr8.4:60545870-60545889 MS.gene95257:CDS 40.0%
CCATTTGTCATGGTTCCATT+GGG - chr8.4:60543963-60543982 None:intergenic 40.0%
CTTTGTAATATGCTCCTCTC+AGG - chr8.4:60545146-60545165 None:intergenic 40.0%
GTTGTAAAGTTGTCCCTGAA+TGG - chr8.4:60544099-60544118 None:intergenic 40.0%
GTTGTTATCTGATGAAACGG+CGG - chr8.4:60545643-60545662 None:intergenic 40.0%
TAAAGGACCAAAGTGGTTGA+CGG + chr8.4:60544352-60544371 MS.gene95257:intron 40.0%
TAAATTACCTTTGGTCCTGC+AGG - chr8.4:60545733-60545752 None:intergenic 40.0%
TCAGGGACAACTTTACAACT+AGG + chr8.4:60544100-60544119 MS.gene95257:intron 40.0%
TCCATTTGTCATGGTTCCAT+TGG - chr8.4:60543964-60543983 None:intergenic 40.0%
TCGCTAAGGACAATCTAGTT+TGG - chr8.4:60543621-60543640 None:intergenic 40.0%
! CAGCACATGTTTTCTTTGGT+TGG - chr8.4:60545669-60545688 None:intergenic 40.0%
! GAACTCGTGTTTTCCATTCA+GGG + chr8.4:60544083-60544102 MS.gene95257:intron 40.0%
! TGAACTCGTGTTTTCCATTC+AGG + chr8.4:60544082-60544101 MS.gene95257:intron 40.0%
!!! CTCGCACAATTTTTTAGAGG+GGG - chr8.4:60545112-60545131 None:intergenic 40.0%
!!! GCTCGCACAATTTTTTAGAG+GGG - chr8.4:60545113-60545132 None:intergenic 40.0%
AAAAAGAGTAACAGTGGCGG+TGG + chr8.4:60545895-60545914 MS.gene95257:CDS 45.0%
AATGCTTGCGGGATAAGCAA+AGG + chr8.4:60545844-60545863 MS.gene95257:CDS 45.0%
CCACTTTGGTCCTTTACGTT+AGG - chr8.4:60544348-60544367 None:intergenic 45.0%
CCCAATGGAACCATGACAAA+TGG + chr8.4:60543960-60543979 MS.gene95257:intron 45.0%
CCTAACGTAAAGGACCAAAG+TGG + chr8.4:60544345-60544364 MS.gene95257:intron 45.0%
GAACTTCCAAAACCCCTCTA+TGG + chr8.4:60545695-60545714 MS.gene95257:CDS 45.0%
GGGAAAAAGAGTAACAGTGG+CGG + chr8.4:60545892-60545911 MS.gene95257:CDS 45.0%
GTTGACGGAGGAAGAGTTAA+GGG + chr8.4:60544367-60544386 MS.gene95257:intron 45.0%
TTGTCGTATGTCTCTCCCAA+TGG + chr8.4:60543945-60543964 MS.gene95257:intron 45.0%
! TTGTGGTAGACCTTGACTTG+AGG + chr8.4:60543346-60543365 MS.gene95257:CDS 45.0%
!!! TAATTTAAGTTTTAAAAAAA+AGG - chr8.4:60544663-60544682 None:intergenic 5.0%
AATATGCTCCTCTCAGGCAG+CGG - chr8.4:60545140-60545159 None:intergenic 50.0%
AGTCACTGTGCAATGCTTGC+GGG + chr8.4:60545833-60545852 MS.gene95257:intron 50.0%
ATTACCTTTGGTCCTGCAGG+AGG - chr8.4:60545730-60545749 None:intergenic 50.0%
CAGTCACTGTGCAATGCTTG+CGG + chr8.4:60545832-60545851 MS.gene95257:intron 50.0%
CTTCATCCCTCCTCAAGTCA+AGG - chr8.4:60543359-60543378 None:intergenic 50.0%
GGTTGACGGAGGAAGAGTTA+AGG + chr8.4:60544366-60544385 MS.gene95257:intron 50.0%
TCATAGCCATGGGGTAGCAT+TGG + chr8.4:60544745-60544764 MS.gene95257:intron 50.0%
TCTTCCTCCGTCAACCACTT+TGG - chr8.4:60544362-60544381 None:intergenic 50.0%
! GGTAGACCTTGACTTGAGGA+GGG + chr8.4:60543350-60543369 MS.gene95257:CDS 50.0%
! TGGTAGACCTTGACTTGAGG+AGG + chr8.4:60543349-60543368 MS.gene95257:CDS 50.0%
AAAACCCCTCTATGGCGTGG+TGG + chr8.4:60545703-60545722 MS.gene95257:CDS 55.0%
AAACCTGAACCCCTGAGAGC+AGG + chr8.4:60544711-60544730 MS.gene95257:intron 55.0%
AACCTGAACCCCTGAGAGCA+GGG + chr8.4:60544712-60544731 MS.gene95257:intron 55.0%
AAGAGTAACAGTGGCGGTGG+CGG + chr8.4:60545898-60545917 MS.gene95257:CDS 55.0%
AGGACCAAAGTGGTTGACGG+AGG + chr8.4:60544355-60544374 MS.gene95257:intron 55.0%
CTGATGAAACGGCGGCGTTA+GGG - chr8.4:60545635-60545654 None:intergenic 55.0%
TCCAAAACCCCTCTATGGCG+TGG + chr8.4:60545700-60545719 MS.gene95257:CDS 55.0%
TCTGATGAAACGGCGGCGTT+AGG - chr8.4:60545636-60545655 None:intergenic 55.0%
! ACCACGCCATAGAGGGGTTT+TGG - chr8.4:60545704-60545723 None:intergenic 55.0%
!! CGGTTGGCTTCATAGCCATG+GGG + chr8.4:60544736-60544755 MS.gene95257:intron 55.0%
!! GCGGTTGGCTTCATAGCCAT+GGG + chr8.4:60544735-60544754 MS.gene95257:intron 55.0%
ACCTGAACCCCTGAGAGCAG+GGG + chr8.4:60544713-60544732 MS.gene95257:intron 60.0%
AGGACCACCACGCCATAGAG+GGG - chr8.4:60545710-60545729 None:intergenic 60.0%
GAGGACCACCACGCCATAGA+GGG - chr8.4:60545711-60545730 None:intergenic 60.0%
GGGTAGCCAATGCTACCCCA+TGG - chr8.4:60544754-60544773 None:intergenic 60.0%
TGGTCCTCCTGCAGGACCAA+AGG + chr8.4:60545723-60545742 MS.gene95257:CDS 60.0%
! GGTGGCGGTGGAAGCAAAGT+TGG + chr8.4:60545913-60545932 MS.gene95257:CDS 60.0%
!! GGCGGTTGGCTTCATAGCCA+TGG + chr8.4:60544734-60544753 MS.gene95257:intron 60.0%
GAGCTTCACCGCTGCCTGAG+AGG + chr8.4:60545129-60545148 MS.gene95257:intron 65.0%
GGAGGACCACCACGCCATAG+AGG - chr8.4:60545712-60545731 None:intergenic 65.0%
TGAACCCCTGAGAGCAGGGG+CGG + chr8.4:60544716-60544735 MS.gene95257:intron 65.0%
! AGTAACAGTGGCGGTGGCGG+TGG + chr8.4:60545901-60545920 MS.gene95257:CDS 65.0%
AGCCAACCGCCCCTGCTCTC+AGG - chr8.4:60544725-60544744 None:intergenic 70.0%
CCAACCGCCCCTGCTCTCAG+GGG - chr8.4:60544723-60544742 None:intergenic 70.0%
GCCAACCGCCCCTGCTCTCA+GGG - chr8.4:60544724-60544743 None:intergenic 70.0%
GCCCCTGCTCTCAGGGGTTC+AGG - chr8.4:60544717-60544736 None:intergenic 70.0%
TGGCGTGGTGGTCCTCCTGC+AGG + chr8.4:60545715-60545734 MS.gene95257:CDS 70.0%
CCCCTGAGAGCAGGGGCGGT+TGG + chr8.4:60544720-60544739 MS.gene95257:intron 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 60543221 60545999 60543221 ID=MS.gene95257
chr8.4 mRNA 60543221 60545999 60543221 ID=MS.gene95257.t1;Parent=MS.gene95257
chr8.4 exon 60543221 60543263 60543221 ID=MS.gene95257.t1.exon1;Parent=MS.gene95257.t1
chr8.4 CDS 60543221 60543263 60543221 ID=cds.MS.gene95257.t1;Parent=MS.gene95257.t1
chr8.4 exon 60543340 60543391 60543340 ID=MS.gene95257.t1.exon2;Parent=MS.gene95257.t1
chr8.4 CDS 60543340 60543391 60543340 ID=cds.MS.gene95257.t1;Parent=MS.gene95257.t1
chr8.4 exon 60545609 60545744 60545609 ID=MS.gene95257.t1.exon3;Parent=MS.gene95257.t1
chr8.4 CDS 60545609 60545744 60545609 ID=cds.MS.gene95257.t1;Parent=MS.gene95257.t1
chr8.4 exon 60545835 60545999 60545835 ID=MS.gene95257.t1.exon4;Parent=MS.gene95257.t1
chr8.4 CDS 60545835 60545999 60545835 ID=cds.MS.gene95257.t1;Parent=MS.gene95257.t1
Gene Sequence

>MS.gene95257

ATGTCACGCACCCAAACCCATTTAACTCTATCAATTTCTTTGTGTTAATTATAATGTTGTTTCTGGATTTAGATCCTCTTTAAGTTGATAATTTTTAATTAGATATTCCTAATTATTAGGGAAAGTTGTGGTAGACCTTGACTTGAGGAGGGATGAAGAGAAATCACTCAAGTCTAAACGAAATTCTTGCCTTTCCACTCATTCACAAGAATATTAATAGAACTCTCTTTTTTTTTTATCTTTAGTAATTTTCTAAATAGTAAGATAATATTTTTTTAGGTGTTTTTCGTTTATACTTCTCCTTGTTTCCCATATTTATACTTTCATTTATACTTTTGGTTAGTTCTTTGCCTTTCATATTGTTTTGAAGTATTTTCCCTATTCATAAAATAGTTTTCCAAACTAGATTGTCCTTAGCGAATTGTGTAATATTCTCGTTTGGTTTAATCTTGATTATTTATCATAGGTTTTACCTATTAGTTTAATAAAATCCGAAACATGTCAAATTATTAAGAGGTTTGGTACAATTTAGATTATTGAAACCAAAATATATTTTTTATGGTCCAAGTTATTAGGAGTATTTTTAAAGAGTGTCTTGTAGTACTCGTCAACAAGACCTAAAGAAAAATTCTCATTAATTATTAAAGTGGTATTATTAATATTTTTACAATTGTTCCTAAGAAAATTTTAACTCGAAAAAAAAAAACTGATCATTTGGTAATTTTTGTCGTATGTCTCTCCCAATGGAACCATGACAAATGGATCAAACCATATCATATTTTTTCTGGTCAAGTACAAACCATATCATATCATATTATTCTCTCAAAAAGGAATCCAAATTCATCGTTTTAGTGACTCATGTGAACTCGTGTTTTCCATTCAGGGACAACTTTACAACTAGGCTTAATTACTCTTTTGGTACCTTAACTTATTTTTTGGTTTCGTTTTGGTCCCTTAAGTTATTAACGTTACAATTTGGTCCCTTAACTTACTTTTTGGTTTTGTTTTGGTCCCTTAACTATTAAAAGTTTCGTTTTGGTCCCTTAACTTATTTTTTTGTTTCGTTTTGGTCCCTTAACTTATTTTTTGGTTTCGTTTTGGTCCCTTAACTCTAATACATTCATCCTAACGTAAAGGACCAAAGTGGTTGACGGAGGAAGAGTTAAGGGACCAAAACGAAATCAAAAAATAAGTTAAGGACCAAAACGAAACCAAAAAATAAGTTAAGGGACCAAAACGAAACTTTTAATAGTTAAGGGACCAAAACGAAACCAAAAAGTAACTTAAGGGACCAAATTGTAACATTAATAACTTATGGGACCAAAATGTAACCAACAAATAACTTAAGGGACCAAATCATTAATTAAGCCTAAAATCTAATAAACGAGAGAAAATCTTACAAGGCAATCTATTCTCAAAACCAACATATACGTTTCATACCTTTTTTTTAAAACTTAAATTAGCCATCAAAATTAATACCATGAAAAATCAAACCTGAACCCCTGAGAGCAGGGGCGGTTGGCTTCATAGCCATGGGGTAGCATTGGCTACCCCAAAAGAAGAATTTTTTTTGAATAACTGGTATAGGTTTATATGTAGCTACCCCATCAATATTTTATTTGGTTACTTCAAACAATTTTGTTGGTTTAAAGGATGATGATATTGCACAAATATTGATAATATATTTAGTTTAGTGTCAATTATGATTAATTTACGTTCTTACAACTCCATAAAGCTCAACTTTTATAAATAACTATTGTTTAACTTTTATTTTTTTCGTTGTCGTTTCATTAAATATAAGTAAAAATTATTGTAAATTTATTTTACTTATTGATTAAGTTTAATATATTAGTTGTTAAAATATATTTTATTTCTTCTTTATATTTGGCCCCCTCTAAAAAATTGTGCGAGCTTCACCGCTGCCTGAGAGGAGCATATTACAAAGTTTCATATCAATTTATAACTTGATGTTTTTTATTATACGTGTGATTTACTGATTTTTAGAAGTTTTTATAATCTATAATAAATATCACCGGAGAAAGTATCTAAGAATACATGAACGAGATGATGAAAGATGTCTATTGGTTTAATCAAACATTCTTCTAAACTTGAATCAAATTATAAAAATGCAACAACATTAAATCTCTTAAATTAGAGATTTGTGATCCTACTCAACTTGATAAATTGCACCAAAAAGGAAATTTAACTAACACACAATAAAGACATGAGGGAGAAATGATTTAATAATGAAATGAAATGATCACAGTGATAAGGTAAGTATTATTGTTGATCACTTAATTAACCTAATCACACACTTCACTTCAACCATTCATGATTTAATAGTTCAAATCACTAATCAATCTTGTTTTGTTCATTTTTCATTTTCAGGTATCAGAAGGTGAAGATTCAAACCCTAACGCCGCCGTTTCATCAGATAACAACAATCCAACCAAAGAAAACATGTGCTGATTGCGGAACTTCCAAAACCCCTCTATGGCGTGGTGGTCCTCCTGCAGGACCAAAGGTAATTTAATTAATCACTAATCAATCGATTTGATGATTGATCACACTAATTTTATTCTTGATCTGATCTAATATGTGATTAATTATGCAGTCACTGTGCAATGCTTGCGGGATAAGCAAAGGAAACAACGAAGAAGGAACAAGAAAGGGGAAAAAGAGTAACAGTGGCGGTGGCGGTGGAAGCAAAGTTGGTGATAATTTGAACATGAAGCAGAGATTATTGAATTTAGGGAAAGAAGTGTTTATGAATCAATGA
Protein sequence

>MS.gene95257.t1

MSRTQTHLTLSISLWKVVVDLDLRRDEEKSLKYQKVKIQTLTPPFHQITTIQPKKTCADCGTSKTPLWRGGPPAGPKSLCNACGISKGNNEEGTRKGKKSNSGGGGGSKVGDNLNMKQRLLNLGKEVFMNQ