Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95375.t1 | XP_003625416.1 | 68.3 | 126 | 14 | 2 | 1 | 100 | 1 | 126 | 3.20E-33 | 151.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95375.t1 | F4JZT3 | 43.4 | 113 | 39 | 2 | 13 | 100 | 11 | 123 | 3.3e-15 | 82.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95375.t1 | G7KYH3 | 68.3 | 126 | 14 | 2 | 1 | 100 | 1 | 126 | 2.3e-33 | 151.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene95375.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95375.t1 | MTR_7g098910 | 68.254 | 126 | 14 | 2 | 1 | 100 | 1 | 126 | 5.00e-51 | 159 |
| MS.gene95375.t1 | MTR_7g099500 | 46.610 | 118 | 36 | 3 | 13 | 103 | 4 | 121 | 6.45e-22 | 85.1 |
| MS.gene95375.t1 | MTR_8g063580 | 41.964 | 112 | 38 | 2 | 14 | 98 | 6 | 117 | 1.63e-17 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95375.t1 | AT5G26594 | 43.363 | 113 | 39 | 2 | 13 | 100 | 11 | 123 | 2.80e-22 | 86.3 |
Find 29 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAATAAGTGTTGTTAATT+CGG | 0.276668 | 3.3:+48163478 | None:intergenic |
| AAGGAATCCAATAGAGGTTT+TGG | 0.280888 | 3.3:-48162639 | MS.gene95375:CDS |
| TCTCGTATTAGAATCTTCTT+TGG | 0.303212 | 3.3:-48162670 | MS.gene95375:intron |
| GGCGTAGCCAAAACCTCTAT+TGG | 0.317821 | 3.3:+48162632 | None:intergenic |
| TCGAATTAATCACACCAATA+TGG | 0.343266 | 3.3:+48163030 | None:intergenic |
| CTTATGGAACCTCAAAATTC+AGG | 0.357949 | 3.3:-48163503 | MS.gene95375:CDS |
| AGGGAAAGGGAAAGGGCTTA+TGG | 0.360531 | 3.3:-48163519 | MS.gene95375:CDS |
| TATGAGTGTAGCATCTTTCC+AGG | 0.378218 | 3.3:-48162772 | MS.gene95375:intron |
| AAACAAGAATTTATGAAAGC+TGG | 0.381997 | 3.3:-48163082 | MS.gene95375:CDS |
| ACTAAGAAACTGCGAGCAAT+TGG | 0.424462 | 3.3:-48163151 | MS.gene95375:CDS |
| AGTCAAACCATTGACCATAT+TGG | 0.426696 | 3.3:-48163044 | MS.gene95375:intron |
| AAGGAAATGCCTATCATGAA+TGG | 0.455972 | 3.3:-48163318 | MS.gene95375:CDS |
| AAATGGAAGATCAAGGGAAA+GGG | 0.471978 | 3.3:-48163532 | MS.gene95375:CDS |
| AATCACACCAATATGGTCAA+TGG | 0.474964 | 3.3:+48163037 | None:intergenic |
| ACCTCAATCCCATTCATGAT+AGG | 0.485829 | 3.3:+48163309 | None:intergenic |
| AACAAGAATTTATGAAAGCT+GGG | 0.487065 | 3.3:-48163081 | MS.gene95375:CDS |
| TTGCACAAAATGGAAGATCA+AGG | 0.493138 | 3.3:-48163539 | None:intergenic |
| AGGAAATGCCTATCATGAAT+GGG | 0.507526 | 3.3:-48163317 | MS.gene95375:CDS |
| GCCTATCATGAATGGGATTG+AGG | 0.520152 | 3.3:-48163310 | MS.gene95375:intron |
| GGTATTCGTAGCATGATTGT+TGG | 0.523765 | 3.3:-48163130 | MS.gene95375:CDS |
| GAAGATCAAGGGAAAGGGAA+AGG | 0.532581 | 3.3:-48163527 | MS.gene95375:CDS |
| CTTGTTCGAATGATTCATCA+AGG | 0.541897 | 3.3:-48163443 | MS.gene95375:CDS |
| AAGATCAAGGGAAAGGGAAA+GGG | 0.547192 | 3.3:-48163526 | MS.gene95375:CDS |
| TATAACAAGGAATCCAATAG+AGG | 0.554761 | 3.3:-48162645 | MS.gene95375:CDS |
| AAAATGGAAGATCAAGGGAA+AGG | 0.580412 | 3.3:-48163533 | MS.gene95375:CDS |
| GAAAGATGCTACACTCATAG+AGG | 0.586600 | 3.3:+48162776 | None:intergenic |
| TGCACAAAATGGAAGATCAA+GGG | 0.596582 | 3.3:-48163538 | None:intergenic |
| CTACACTCATAGAGGACTCG+TGG | 0.614666 | 3.3:+48162784 | None:intergenic |
| ATCTTCTTTGGAGTATAACA+AGG | 0.621968 | 3.3:-48162658 | MS.gene95375:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATAGATTTATCTCTTTA+GGG | - | chr3.3:48163292-48163311 | MS.gene95375:intron | 15.0% |
| !! | AAAAATGAAGCTGTAAAAAA+TGG | - | chr3.3:48162751-48162770 | MS.gene95375:intron | 20.0% |
| !! | GTATATAGATTTATCTCTTT+AGG | - | chr3.3:48163291-48163310 | MS.gene95375:intron | 20.0% |
| !! | TTAAAATAAGATTCAATCGT+TGG | - | chr3.3:48162957-48162976 | MS.gene95375:intron | 20.0% |
| !!! | AAAGATTTTAATTATTCTCG+TGG | - | chr3.3:48162806-48162825 | MS.gene95375:CDS | 20.0% |
| !!! | CTATATACTTCAATTTTAGT+AGG | + | chr3.3:48163279-48163298 | None:intergenic | 20.0% |
| !!! | TACAATAAGTGTTGTTAATT+CGG | + | chr3.3:48162674-48162693 | None:intergenic | 20.0% |
| !!! | TTTTTTTCTGTATAATACGT+TGG | - | chr3.3:48162895-48162914 | MS.gene95375:intron | 20.0% |
| ! | AAACAAGAATTTATGAAAGC+TGG | - | chr3.3:48163067-48163086 | MS.gene95375:CDS | 25.0% |
| ! | AACAAGAATTTATGAAAGCT+GGG | - | chr3.3:48163068-48163087 | MS.gene95375:CDS | 25.0% |
| ! | TTTAATTATTCTCGTGGATA+AGG | - | chr3.3:48162812-48162831 | MS.gene95375:intron | 25.0% |
| !! | TTTTACAACATCATTCTCAA+AGG | + | chr3.3:48163179-48163198 | None:intergenic | 25.0% |
| !!! | ACATTTTCATTGACTAACTT+AGG | - | chr3.3:48163323-48163342 | MS.gene95375:intron | 25.0% |
| AACTATACCAAGATGAATCT+TGG | - | chr3.3:48163407-48163426 | MS.gene95375:intron | 30.0% | |
| AACTATACCAAGATTCATCT+TGG | + | chr3.3:48163417-48163436 | None:intergenic | 30.0% | |
| ATTCTTGATGTCATAAAAGC+TGG | - | chr3.3:48163141-48163160 | MS.gene95375:CDS | 30.0% | |
| TATAACAAGGAATCCAATAG+AGG | - | chr3.3:48163504-48163523 | MS.gene95375:CDS | 30.0% | |
| TCGAATTAATCACACCAATA+TGG | + | chr3.3:48163122-48163141 | None:intergenic | 30.0% | |
| TCTCGTATTAGAATCTTCTT+TGG | - | chr3.3:48163479-48163498 | MS.gene95375:CDS | 30.0% | |
| TGAAGCTGTAAAAAATGGTA+AGG | - | chr3.3:48162756-48162775 | MS.gene95375:intron | 30.0% | |
| TGTATAATACGTTGGAAAGA+AGG | - | chr3.3:48162903-48162922 | MS.gene95375:intron | 30.0% | |
| TTCTTGATGTCATAAAAGCT+GGG | - | chr3.3:48163142-48163161 | MS.gene95375:CDS | 30.0% | |
| TTGGTATAGTTTATACTACC+TGG | + | chr3.3:48163398-48163417 | None:intergenic | 30.0% | |
| !! | ATCTTCTTTGGAGTATAACA+AGG | - | chr3.3:48163491-48163510 | MS.gene95375:CDS | 30.0% |
| AAGGAAATGCCTATCATGAA+TGG | - | chr3.3:48162831-48162850 | MS.gene95375:intron | 35.0% | |
| AATCACACCAATATGGTCAA+TGG | + | chr3.3:48163115-48163134 | None:intergenic | 35.0% | |
| AGGAAATGCCTATCATGAAT+GGG | - | chr3.3:48162832-48162851 | MS.gene95375:intron | 35.0% | |
| AGTCAAACCATTGACCATAT+TGG | - | chr3.3:48163105-48163124 | MS.gene95375:CDS | 35.0% | |
| CTTATGGAACCTCAAAATTC+AGG | - | chr3.3:48162646-48162665 | MS.gene95375:CDS | 35.0% | |
| CTTGTTCGAATGATTCATCA+AGG | - | chr3.3:48162706-48162725 | MS.gene95375:intron | 35.0% | |
| ! | AAGGAATCCAATAGAGGTTT+TGG | - | chr3.3:48163510-48163529 | MS.gene95375:CDS | 35.0% |
| ! | AATTCGGATCCTGAATTTTG+AGG | + | chr3.3:48162658-48162677 | None:intergenic | 35.0% |
| !!! | TCGTTGGTTGTTTTTTATGC+AGG | - | chr3.3:48162973-48162992 | MS.gene95375:intron | 35.0% |
| AAGATCAAGGGAAAGGGAAA+GGG | - | chr3.3:48162623-48162642 | MS.gene95375:CDS | 40.0% | |
| ACCTCAATCCCATTCATGAT+AGG | + | chr3.3:48162843-48162862 | None:intergenic | 40.0% | |
| ACTAAGAAACTGCGAGCAAT+TGG | - | chr3.3:48162998-48163017 | MS.gene95375:intron | 40.0% | |
| GAAAGATGCTACACTCATAG+AGG | + | chr3.3:48163376-48163395 | None:intergenic | 40.0% | |
| GCTGTAGATAATCATTGCAG+TGG | - | chr3.3:48162781-48162800 | MS.gene95375:CDS | 40.0% | |
| GGTATTCGTAGCATGATTGT+TGG | - | chr3.3:48163019-48163038 | MS.gene95375:intron | 40.0% | |
| TATGAGTGTAGCATCTTTCC+AGG | - | chr3.3:48163377-48163396 | MS.gene95375:intron | 40.0% | |
| GAAGATCAAGGGAAAGGGAA+AGG | - | chr3.3:48162622-48162641 | MS.gene95375:CDS | 45.0% | |
| GCCTATCATGAATGGGATTG+AGG | - | chr3.3:48162839-48162858 | MS.gene95375:intron | 45.0% | |
| AGGGAAAGGGAAAGGGCTTA+TGG | - | chr3.3:48162630-48162649 | MS.gene95375:CDS | 50.0% | |
| CTACACTCATAGAGGACTCG+TGG | + | chr3.3:48163368-48163387 | None:intergenic | 50.0% | |
| GGCGTAGCCAAAACCTCTAT+TGG | + | chr3.3:48163520-48163539 | None:intergenic | 50.0% | |
| !!! | GGTTTTGGCTACGCCAGAAA+TGG | - | chr3.3:48163525-48163544 | MS.gene95375:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 48162619 | 48163552 | 48162619 | ID=MS.gene95375 |
| chr3.3 | mRNA | 48162619 | 48163552 | 48162619 | ID=MS.gene95375.t1;Parent=MS.gene95375 |
| chr3.3 | exon | 48163409 | 48163552 | 48163409 | ID=MS.gene95375.t1.exon1;Parent=MS.gene95375.t1 |
| chr3.3 | CDS | 48163409 | 48163552 | 48163409 | ID=cds.MS.gene95375.t1;Parent=MS.gene95375.t1 |
| chr3.3 | exon | 48163311 | 48163337 | 48163311 | ID=MS.gene95375.t1.exon2;Parent=MS.gene95375.t1 |
| chr3.3 | CDS | 48163311 | 48163337 | 48163311 | ID=cds.MS.gene95375.t1;Parent=MS.gene95375.t1 |
| chr3.3 | exon | 48163045 | 48163176 | 48163045 | ID=MS.gene95375.t1.exon3;Parent=MS.gene95375.t1 |
| chr3.3 | CDS | 48163045 | 48163176 | 48163045 | ID=cds.MS.gene95375.t1;Parent=MS.gene95375.t1 |
| chr3.3 | exon | 48162773 | 48162826 | 48162773 | ID=MS.gene95375.t1.exon4;Parent=MS.gene95375.t1 |
| chr3.3 | CDS | 48162773 | 48162826 | 48162773 | ID=cds.MS.gene95375.t1;Parent=MS.gene95375.t1 |
| chr3.3 | exon | 48162619 | 48162681 | 48162619 | ID=MS.gene95375.t1.exon5;Parent=MS.gene95375.t1 |
| chr3.3 | CDS | 48162619 | 48162681 | 48162619 | ID=cds.MS.gene95375.t1;Parent=MS.gene95375.t1 |
| Gene Sequence |
| Protein sequence |
