AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95541

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95541.t1 MTR_4g047580 58.974 429 64 4 1 364 111 492 1.49e-158 462
MS.gene95541.t1 MTR_4g049760 65.017 303 59 3 1 302 119 375 6.69e-122 369
MS.gene95541.t1 MTR_4g049790 49.400 417 87 7 1 346 144 507 1.51e-111 342
MS.gene95541.t1 MTR_2g088000 39.481 347 102 10 114 364 222 556 7.57e-56 197
MS.gene95541.t1 MTR_3g053330 38.839 224 109 4 79 301 139 335 5.59e-40 151
MS.gene95541.t1 MTR_2g023930 34.097 349 125 11 115 368 192 530 1.26e-39 151
MS.gene95541.t1 MTR_7g110720 68.478 92 29 0 202 293 174 265 1.78e-38 145
MS.gene95541.t1 MTR_7g110720 68.478 92 29 0 202 293 201 292 2.16e-38 145
MS.gene95541.t1 MTR_4g132430 63.889 108 39 0 195 302 105 212 4.88e-38 143
MS.gene95541.t1 MTR_4g132430 63.889 108 39 0 195 302 239 346 7.32e-37 142
MS.gene95541.t1 MTR_2g045360 54.386 114 52 0 188 301 182 295 2.14e-34 134
MS.gene95541.t1 MTR_7g009730 48.387 124 58 1 174 291 170 293 3.79e-31 127
MS.gene95541.t1 MTR_4g094412 46.296 162 67 4 202 357 228 375 1.18e-28 119
MS.gene95541.t1 MTR_4g094412 46.296 162 67 4 202 357 219 366 1.22e-28 119
MS.gene95541.t1 MTR_4g107970 60.526 76 30 0 202 277 163 238 2.38e-26 112
MS.gene95541.t1 MTR_4g107970 60.526 76 30 0 202 277 240 315 4.57e-26 112
MS.gene95541.t1 MTR_5g044695 67.925 53 17 0 195 247 55 107 5.57e-21 88.6
MS.gene95541.t1 MTR_7g011820 42.241 116 56 2 174 289 377 481 1.97e-20 94.7
MS.gene95541.t1 MTR_6g038890 67.925 53 17 0 195 247 166 218 4.89e-20 89.4
MS.gene95541.t1 MTR_5g042920 32.749 171 94 4 143 299 238 401 1.17e-19 91.7
MS.gene95541.t1 MTR_4g007060 27.660 188 118 4 194 373 105 282 2.08e-19 89.0
MS.gene95541.t1 MTR_4g007060 27.660 188 118 4 194 373 139 316 4.04e-19 88.6
MS.gene95541.t1 MTR_4g082580 40.171 117 60 3 199 312 260 369 1.60e-18 87.8
MS.gene95541.t1 MTR_3g056100 37.607 117 69 2 190 303 231 346 4.18e-18 87.0
MS.gene95541.t1 MTR_3g056100 37.607 117 69 2 190 303 244 359 4.38e-18 87.0
MS.gene95541.t1 MTR_3g106060 27.336 289 166 10 102 366 20 288 6.74e-18 84.7
MS.gene95541.t1 MTR_3g056100 37.607 117 69 2 190 303 388 503 8.65e-18 87.0
MS.gene95541.t1 MTR_3g056100 37.607 117 69 2 190 303 375 490 9.04e-18 86.7
MS.gene95541.t1 MTR_5g094430 43.956 91 50 1 189 279 202 291 2.04e-17 84.7
MS.gene95541.t1 MTR_5g094430 43.956 91 50 1 189 279 224 313 2.06e-17 85.1
MS.gene95541.t1 MTR_3g009470 48.750 80 40 1 200 279 478 556 3.49e-17 85.1
MS.gene95541.t1 MTR_5g094430 43.956 91 50 1 189 279 336 425 3.85e-17 84.7
MS.gene95541.t1 MTR_1g013760 33.129 163 96 4 202 363 125 275 9.88e-17 80.9
MS.gene95541.t1 MTR_7g080000 50.000 76 37 1 204 279 419 493 1.44e-16 83.2
MS.gene95541.t1 MTR_7g080020 48.718 78 39 1 202 279 478 554 2.18e-16 82.8
MS.gene95541.t1 MTR_7g071120 48.148 81 41 1 199 279 306 385 2.68e-16 82.0
MS.gene95541.t1 MTR_5g074400 41.935 93 53 1 187 279 390 481 4.55e-16 81.6
MS.gene95541.t1 MTR_3g031220 45.679 81 43 1 199 279 347 426 5.35e-16 80.9
MS.gene95541.t1 MTR_7g109600 37.500 112 65 2 196 305 30 138 8.30e-16 77.8
MS.gene95541.t1 MTR_4g013260 46.835 79 41 1 199 277 318 395 1.72e-15 79.3
MS.gene95541.t1 MTR_5g097490 40.217 92 54 1 188 279 223 313 1.75e-15 78.6
MS.gene95541.t1 MTR_2g083870 44.304 79 44 0 208 286 71 149 1.93e-15 76.6
MS.gene95541.t1 MTR_1g013790 45.455 66 36 0 212 277 96 161 4.34e-15 75.5
MS.gene95541.t1 MTR_4g094588 39.048 105 62 2 204 308 165 267 4.59e-15 77.0
MS.gene95541.t1 MTR_2g032030 30.570 193 113 6 134 306 166 357 4.98e-15 77.8
MS.gene95541.t1 MTR_2g105060 43.590 78 42 1 203 278 153 230 5.18e-15 76.3
MS.gene95541.t1 MTR_8g087000 48.485 66 34 0 214 279 74 139 5.25e-15 75.1
MS.gene95541.t1 MTR_8g087000 48.485 66 34 0 214 279 73 138 5.32e-15 75.1
MS.gene95541.t1 MTR_8g096730 34.906 106 68 1 175 280 28 132 5.47e-15 72.8
MS.gene95541.t1 MTR_2g105060 43.590 78 42 1 203 278 78 155 5.78e-15 75.1
MS.gene95541.t1 MTR_7g109800 41.489 94 50 2 202 295 130 218 7.10e-15 75.9
MS.gene95541.t1 MTR_2g016770 50.000 64 32 0 214 277 32 95 7.19e-15 74.3
MS.gene95541.t1 MTR_2g016770 50.000 64 32 0 214 277 24 87 8.01e-15 73.9
MS.gene95541.t1 MTR_2g032030 30.636 173 100 5 134 287 166 337 8.40e-15 77.0
MS.gene95541.t1 MTR_7g079010 45.570 79 42 1 196 274 120 197 8.93e-15 73.9
MS.gene95541.t1 MTR_2g033820 36.364 110 62 3 202 307 188 293 2.20e-14 75.1
MS.gene95541.t1 MTR_4g122530 44.000 75 41 1 203 277 194 267 3.16e-14 75.1
MS.gene95541.t1 MTR_5g091390 44.444 81 44 1 204 284 144 223 4.13e-14 73.6
MS.gene95541.t1 MTR_8g098945 39.326 89 53 1 216 304 128 215 6.21e-14 72.4
MS.gene95541.t1 MTR_7g028415 48.571 70 35 1 207 276 83 151 1.03e-13 69.7
MS.gene95541.t1 MTR_7g028710 48.571 70 35 1 207 276 83 151 1.03e-13 69.7
MS.gene95541.t1 MTR_7g100510 43.210 81 45 1 197 277 341 420 1.30e-13 73.2
MS.gene95541.t1 MTR_1g099600 43.836 73 41 0 215 287 183 255 1.38e-13 72.0
MS.gene95541.t1 MTR_7g079040 45.333 75 41 0 213 287 150 224 1.44e-13 72.4
MS.gene95541.t1 MTR_1g077780 42.308 78 44 1 202 279 47 123 1.74e-13 68.2
MS.gene95541.t1 MTR_1g086790 40.000 80 47 1 202 281 143 221 3.69e-13 70.9
MS.gene95541.t1 MTR_3g104750 45.070 71 38 1 204 274 91 160 4.79e-13 67.4
MS.gene95541.t1 MTR_1g015140 45.205 73 39 1 202 274 87 158 5.35e-13 67.4
MS.gene95541.t1 MTR_1g009613 43.529 85 46 1 202 284 245 329 9.82e-13 69.7
MS.gene95541.t1 MTR_2g024350 38.961 77 47 0 212 288 3 79 1.31e-12 67.8
MS.gene95541.t1 MTR_5g043880 42.308 78 44 1 197 274 104 180 2.74e-12 67.8
MS.gene95541.t1 MTR_8g028565 42.667 75 43 0 213 287 151 225 3.18e-12 68.2
MS.gene95541.t1 MTR_8g092010 42.857 77 43 1 204 280 95 170 3.22e-12 65.9
MS.gene95541.t1 MTR_3g095040 43.590 78 43 1 203 280 104 180 5.77e-12 65.5
MS.gene95541.t1 MTR_4g094908 48.387 62 32 0 218 279 267 328 1.05e-11 66.6
MS.gene95541.t1 MTR_2g024380 38.961 77 47 0 212 288 246 322 2.43e-11 66.2
MS.gene95541.t1 MTR_1g070455 49.123 57 29 0 218 274 294 350 2.92e-11 65.5
MS.gene95541.t1 MTR_1g078260 45.833 72 37 2 204 274 234 304 4.36e-11 65.5
MS.gene95541.t1 MTR_5g016610 48.333 60 31 0 217 276 199 258 5.25e-11 63.9
MS.gene95541.t1 MTR_8g007270 41.772 79 45 1 196 274 133 210 8.00e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95541.t1 AT4G04450 44.651 215 89 5 97 299 84 280 4.53e-44 161
MS.gene95541.t1 AT4G04450 44.651 215 89 5 97 299 178 374 1.79e-43 162
MS.gene95541.t1 AT1G62300 66.981 106 35 0 196 301 291 396 1.76e-36 142
MS.gene95541.t1 AT4G22070 68.293 82 26 0 196 277 276 357 1.39e-34 137
MS.gene95541.t1 AT4G22070 68.293 82 26 0 196 277 276 357 1.39e-34 137
MS.gene95541.t1 AT4G22070 68.293 82 26 0 196 277 276 357 1.39e-34 137
MS.gene95541.t1 AT4G22070 68.293 82 26 0 196 277 276 357 1.39e-34 137
MS.gene95541.t1 AT1G68150 42.949 156 75 2 161 302 165 320 7.48e-33 129
MS.gene95541.t1 AT5G15130 60.000 90 36 0 202 291 170 259 2.45e-31 127
MS.gene95541.t1 AT5G15130 60.000 90 36 0 202 291 212 301 3.11e-31 127
MS.gene95541.t1 AT4G01720 45.455 132 65 2 151 277 170 299 1.35e-30 125
MS.gene95541.t1 AT1G18860 29.677 310 155 6 2 295 55 317 8.21e-28 117
MS.gene95541.t1 AT1G69810 54.369 103 46 1 190 291 118 220 4.58e-27 112
MS.gene95541.t1 AT1G69810 54.369 103 46 1 190 291 176 278 5.55e-27 112
MS.gene95541.t1 AT2G25000 32.667 150 99 2 132 280 40 188 1.82e-18 85.5
MS.gene95541.t1 AT1G13960 49.367 79 39 1 199 277 391 468 2.01e-18 88.6
MS.gene95541.t1 AT1G13960 49.367 79 39 1 199 277 238 315 2.72e-18 87.0
MS.gene95541.t1 AT2G03340 29.565 230 124 7 74 279 261 476 2.78e-18 88.2
MS.gene95541.t1 AT2G25000 29.648 199 133 4 84 280 16 209 2.95e-18 85.5
MS.gene95541.t1 AT2G25000 32.667 150 99 2 132 280 18 166 2.98e-18 84.3
MS.gene95541.t1 AT2G25000 32.667 150 99 2 132 280 18 166 2.98e-18 84.3
MS.gene95541.t1 AT3G01080 43.023 86 48 1 192 277 319 403 2.13e-17 85.1
MS.gene95541.t1 AT3G01080 43.529 85 47 1 193 277 282 365 2.85e-17 84.3
MS.gene95541.t1 AT4G23550 32.515 163 88 3 212 355 135 294 2.85e-17 83.2
MS.gene95541.t1 AT1G29860 36.937 111 65 2 202 312 121 226 7.92e-17 81.3
MS.gene95541.t1 AT4G26640 40.659 91 53 1 189 279 177 266 8.49e-17 82.8
MS.gene95541.t1 AT4G26640 40.659 91 53 1 189 279 177 266 8.49e-17 82.8
MS.gene95541.t1 AT4G26640 40.659 91 53 1 189 279 177 266 8.49e-17 82.8
MS.gene95541.t1 AT4G26640 40.659 91 53 1 189 279 177 266 8.49e-17 82.8
MS.gene95541.t1 AT4G26640 40.659 91 53 1 189 279 313 402 9.47e-17 83.6
MS.gene95541.t1 AT5G56270 47.500 80 41 1 200 279 470 548 1.01e-16 84.0
MS.gene95541.t1 AT4G26640 40.659 91 53 1 189 279 281 370 1.11e-16 83.2
MS.gene95541.t1 AT2G38470 46.914 81 42 1 199 279 344 423 1.21e-16 83.2
MS.gene95541.t1 AT4G26640 40.659 91 53 1 189 279 353 442 1.23e-16 83.2
MS.gene95541.t1 AT1G80840 27.586 174 104 5 203 363 130 294 2.37e-16 80.1
MS.gene95541.t1 AT4G31800 38.095 105 60 2 196 295 85 189 2.57e-16 79.0
MS.gene95541.t1 AT4G31800 38.095 105 60 2 196 295 150 254 4.12e-16 79.7
MS.gene95541.t1 AT2G04880 38.710 93 56 1 187 279 253 344 8.54e-16 80.1
MS.gene95541.t1 AT2G04880 38.710 93 56 1 187 279 277 368 9.25e-16 80.1
MS.gene95541.t1 AT1G64000 50.000 76 37 1 199 274 96 170 9.40e-16 76.3
MS.gene95541.t1 AT5G46350 40.000 95 56 1 204 298 170 263 1.39e-15 78.2
MS.gene95541.t1 AT2G30250 43.023 86 48 1 196 281 307 391 2.53e-15 78.2
MS.gene95541.t1 AT5G41570 50.000 72 35 1 203 274 84 154 2.80e-15 74.3
MS.gene95541.t1 AT5G07100 37.190 121 65 3 171 281 85 204 3.83e-15 75.1
MS.gene95541.t1 AT5G07100 37.190 121 65 3 171 281 85 204 3.83e-15 75.1
MS.gene95541.t1 AT5G07100 37.190 121 65 3 171 281 85 204 3.83e-15 75.1
MS.gene95541.t1 AT5G07100 37.190 121 65 3 171 281 85 204 3.83e-15 75.1
MS.gene95541.t1 AT4G18170 46.753 77 40 1 202 278 157 232 4.60e-15 76.6
MS.gene95541.t1 AT5G07100 38.017 121 64 3 171 281 178 297 5.71e-15 76.3
MS.gene95541.t1 AT5G13080 49.296 71 35 1 204 274 54 123 7.54e-15 72.4
MS.gene95541.t1 AT2G46130 46.479 71 37 1 204 274 17 86 9.70e-15 70.9
MS.gene95541.t1 AT2G47260 37.069 116 62 3 202 307 159 273 1.27e-14 75.5
MS.gene95541.t1 AT5G26170 47.222 72 37 1 203 274 99 169 1.99e-14 72.0
MS.gene95541.t1 AT4G26440 46.053 76 40 1 204 279 359 433 2.01e-14 76.3
MS.gene95541.t1 AT1G29280 45.714 70 38 0 208 277 65 134 2.13e-14 73.6
MS.gene95541.t1 AT5G52830 42.683 82 47 0 206 287 154 235 4.79e-14 73.9
MS.gene95541.t1 AT3G58710 43.836 73 41 0 208 280 61 133 4.84e-14 72.8
MS.gene95541.t1 AT3G58710 43.836 73 41 0 208 280 62 134 5.04e-14 72.8
MS.gene95541.t1 AT1G30650 42.857 84 47 1 202 284 201 284 6.70e-14 74.3
MS.gene95541.t1 AT4G30935 36.134 119 71 2 185 303 299 412 6.72e-14 74.3
MS.gene95541.t1 AT5G49520 41.250 80 46 1 202 281 206 284 7.29e-14 73.9
MS.gene95541.t1 AT2G34830 43.373 83 46 1 203 284 200 282 7.57e-14 73.9
MS.gene95541.t1 AT2G34830 43.373 83 46 1 203 284 209 291 9.23e-14 73.9
MS.gene95541.t1 AT3G01970 47.297 74 38 1 203 276 51 123 1.18e-13 68.9
MS.gene95541.t1 AT4G01250 45.333 75 41 0 213 287 124 198 2.13e-13 71.2
MS.gene95541.t1 AT5G45050 32.374 139 88 2 164 302 994 1126 6.44e-13 72.0
MS.gene95541.t1 AT3G62340 38.889 108 61 3 203 310 104 206 6.80e-13 69.7
MS.gene95541.t1 AT5G64810 44.681 94 48 2 184 274 74 166 7.26e-13 67.8
MS.gene95541.t1 AT5G45050 32.374 139 88 2 164 302 1133 1265 7.42e-13 72.0
MS.gene95541.t1 AT5G45050 32.374 139 88 2 164 302 1105 1237 7.57e-13 72.0
MS.gene95541.t1 AT1G55600 42.105 76 43 1 204 279 294 368 5.59e-12 68.6
MS.gene95541.t1 AT2G30590 48.438 64 32 1 218 280 314 377 2.05e-11 66.2
MS.gene95541.t1 AT2G23320 47.458 59 31 0 218 276 241 299 2.49e-11 65.5
MS.gene95541.t1 AT1G69310 40.000 75 44 1 200 274 130 203 3.37e-11 64.7
MS.gene95541.t1 AT1G69310 40.000 75 44 1 200 274 130 203 3.37e-11 64.7
MS.gene95541.t1 AT1G69310 40.000 75 44 1 200 274 130 203 3.37e-11 64.7
MS.gene95541.t1 AT2G44745 38.462 91 54 2 186 275 113 202 6.08e-11 62.8

Find 94 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAAATCTGCAGGAAAATT+TGG 0.139139 4.3:-51072733 None:intergenic
CCTAACTGATCCTTACTTCT+AGG 0.157547 4.3:-51070856 None:intergenic
ACTGGGGCCAATTAAATTTA+TGG 0.164953 4.3:+51070540 MS.gene95541:CDS
TTAACTATCTGAACAATATT+TGG 0.195632 4.3:-51072691 None:intergenic
CCAAGATCCATAAATTTAAT+TGG 0.204486 4.3:-51070547 None:intergenic
GTATTTGATGGAAACTTTAA+AGG 0.252144 4.3:+51070490 MS.gene95541:CDS
CTTTAACTTCCTTAACATTA+TGG 0.256499 4.3:-51070459 None:intergenic
CCAATTAAATTTATGGATCT+TGG 0.268064 4.3:+51070547 MS.gene95541:CDS
TCTTGGATTGGCTAGTAATA+AGG 0.268615 4.3:+51070564 MS.gene95541:CDS
AGCGATTTCTCCCTCATTAC+TGG 0.281101 4.3:+51074272 MS.gene95541:intron
TATCAATGTCTTCAATAGTT+TGG 0.301131 4.3:-51076919 None:intergenic
AATAAGGGTAAGGAAGTTAA+AGG 0.305666 4.3:+51070580 MS.gene95541:CDS
AGGATTGAAGAATGCAGATT+TGG 0.309972 4.3:+51076139 MS.gene95541:intron
AAGGTTAAAGAAGTGTTTAA+TGG 0.314983 4.3:+51070703 MS.gene95541:CDS
TGCTGGATTGGATACTAATA+AGG 0.317920 4.3:+51070684 MS.gene95541:CDS
CATAATGTTAAGGAAGTTAA+AGG 0.325037 4.3:+51070460 MS.gene95541:CDS
AGTTGAACTTGAGATGATTC+AGG 0.335606 4.3:-51077154 None:intergenic
GGCATGATGAGTTTGGTTAC+AGG 0.337707 4.3:+51076837 MS.gene95541:intron
AAAAGGAAATTTGTTGATGC+TGG 0.371075 4.3:+51070667 MS.gene95541:CDS
GCCATTTCTTTGGCTGCTGC+AGG 0.373631 4.3:-51072325 None:intergenic
GGCTATAGGCATGATGAGTT+TGG 0.375417 4.3:+51076830 MS.gene95541:intron
AACTGAGAATGGTGGAGTAC+TGG 0.377375 4.3:+51070522 MS.gene95541:CDS
AAGTCGTGACGTAGTGTTCA+TGG 0.378321 4.3:+51076892 MS.gene95541:CDS
CTGCAGCGATAATAAGCTTT+AGG 0.378946 4.3:-51071174 None:intergenic
CATCTCAAGTTCAACTTAGA+AGG 0.390697 4.3:+51077161 MS.gene95541:CDS
AGCTAGCCCTTCACAACTAT+TGG 0.400559 4.3:+51072663 MS.gene95541:CDS
AAAGGAAAAGAAGTATTTGA+TGG 0.422383 4.3:+51070478 MS.gene95541:CDS
GAAATTTGTTGATGCTGGAT+TGG 0.422880 4.3:+51070672 MS.gene95541:CDS
TGGTTCTGATTAGGTATTTG+AGG 0.425279 4.3:-51072604 None:intergenic
TATTTGATGGAAACTTTAAA+GGG 0.425379 4.3:+51070491 MS.gene95541:CDS
GACACAAGGTTCACAACACC+TGG 0.431680 4.3:+51074315 MS.gene95541:CDS
GAAGTCAGAATTGCTTCAAT+TGG 0.436101 4.3:+51076229 MS.gene95541:CDS
TAAATTTATGGATCTTGGAT+TGG 0.436594 4.3:+51070552 MS.gene95541:CDS
GATGCAAGACCATAATGTTA+AGG 0.441157 4.3:+51070450 MS.gene95541:CDS
CTTGGATTGGCTAGTAATAA+GGG 0.449698 4.3:+51070565 MS.gene95541:CDS
TCCCTCATTACTGGTCGGAT+CGG 0.453656 4.3:+51074281 MS.gene95541:CDS
CCCTCATTACTGGTCGGATC+GGG 0.456343 4.3:+51074282 MS.gene95541:CDS
ACCTGCAGCAGCCAAAGAAA+TGG 0.462652 4.3:+51072324 MS.gene95541:CDS
GGTTCTGATTAGGTATTTGA+GGG 0.462686 4.3:-51072603 None:intergenic
AATGAGAAAACCTAGAAGTA+AGG 0.465713 4.3:+51070846 MS.gene95541:CDS
CGACGATCCGAGCGGTGAAA+AGG 0.468737 4.3:-51074343 None:intergenic
CCTAGAAGTAAGGATCAGTT+AGG 0.469639 4.3:+51070856 MS.gene95541:CDS
AGTTAGTGCAAAGGCAATGT+AGG 0.470279 4.3:+51070764 MS.gene95541:CDS
ACTGAGAATGGTGGAGTACT+GGG 0.475263 4.3:+51070523 MS.gene95541:CDS
TGGTTGCTCAGTCCGAAAAC+AGG 0.478137 4.3:+51071206 MS.gene95541:CDS
ATGTCACATGATGAAGATAT+TGG 0.499614 4.3:+51077124 MS.gene95541:CDS
AGCAATTCTGACTTCCTTGA+GGG 0.504031 4.3:-51076221 None:intergenic
TTATTGCTTTAGATCACTGA+TGG 0.505356 4.3:+51071102 MS.gene95541:intron
AAGCATCGAGGTTGCTTCGA+AGG 0.510775 4.3:+51070891 MS.gene95541:CDS
ATGATGGAGAATTAGTGAAA+AGG 0.514995 4.3:+51070650 MS.gene95541:CDS
TGTATGATCCTGTAGAGAAA+AGG 0.516242 4.3:+51076861 MS.gene95541:CDS
AAATGTTCTCATTGCATGGC+TGG 0.517558 4.3:+51076164 MS.gene95541:CDS
AGAGAAGCTTACAAATCTGC+AGG 0.517768 4.3:-51072742 None:intergenic
CGATAATAAGCTTTAGGACA+CGG 0.531100 4.3:-51071168 None:intergenic
GGTATTGCCAATAGTTGTGA+AGG 0.534263 4.3:-51072670 None:intergenic
ATTGGCTAGTAATAAGGGTA+AGG 0.543564 4.3:+51070570 MS.gene95541:CDS
AACTATTGAAGACATTGATA+AGG 0.552030 4.3:+51076922 MS.gene95541:CDS
GCCCGATCCGACCAGTAATG+AGG 0.555403 4.3:-51074283 None:intergenic
AGATCACTGATGGATGTCAA+TGG 0.557337 4.3:+51071112 MS.gene95541:intron
CTGCAGGTAACAAGTGGTTG+TGG 0.557747 4.3:-51072309 None:intergenic
AAAGGACAAGAAGTTTCTGA+TGG 0.557815 4.3:+51070598 MS.gene95541:CDS
AAAATTTGGATTAGCAGCAA+TGG 0.568779 4.3:-51072719 None:intergenic
TATCGCTGCAGCACATCCCA+TGG 0.572408 4.3:+51071186 MS.gene95541:CDS
CCCGATCCGACCAGTAATGA+GGG 0.573393 4.3:-51074282 None:intergenic
GATAATAAGCTTTAGGACAC+GGG 0.575823 4.3:-51071167 None:intergenic
AAGCAATTCTGACTTCCTTG+AGG 0.582145 4.3:-51076222 None:intergenic
GTTAGTGCAAAGGCAATGTA+GGG 0.584846 4.3:+51070765 MS.gene95541:CDS
GGAGAAATGTTCTCATTGCA+TGG 0.585377 4.3:+51076160 MS.gene95541:CDS
TCAAAAGACATCCTCCCTCA+AGG 0.587779 4.3:+51076207 MS.gene95541:CDS
TGCGGACATAAATTGTCGAA+AGG 0.592856 4.3:+51071141 MS.gene95541:CDS
TACTAATGCTGAGACTGCGA+TGG 0.595069 4.3:+51070801 MS.gene95541:CDS
ATTGATGATGATATTCAGAA+TGG 0.596765 4.3:+51077025 MS.gene95541:CDS
GTATTGCCAATAGTTGTGAA+GGG 0.598828 4.3:-51072669 None:intergenic
ATTTCTCCCTCATTACTGGT+CGG 0.602673 4.3:+51074276 MS.gene95541:CDS
AATTCTGACTTCCTTGAGGG+AGG 0.604951 4.3:-51076218 None:intergenic
TACTCCTCCTCATCACTAGT+GGG 0.607968 4.3:-51077100 None:intergenic
TGGCTGCTGCAGGTAACAAG+TGG 0.612014 4.3:-51072315 None:intergenic
CCGCTCGGATCGTCGAAACA+AGG 0.613818 4.3:+51074351 MS.gene95541:CDS
TTCCAACTTAACAGGTACAA+AGG 0.616787 4.3:+51072242 MS.gene95541:intron
ATACTCCTCCTCATCACTAG+TGG 0.618946 4.3:-51077101 None:intergenic
TGTTCCCACTAGTGATGAGG+AGG 0.630313 4.3:+51077096 MS.gene95541:CDS
AAGAAGCATCTTTGCAGCTG+CGG 0.630421 4.3:-51072359 None:intergenic
TGTTAATGTTGATAATGTTG+AGG 0.631518 4.3:+51070939 MS.gene95541:CDS
AGCTAGATCAGAAGAAACCA+TGG 0.635656 4.3:+51070993 MS.gene95541:CDS
GAATAGATTACGATTACATG+CGG 0.647686 4.3:+51070410 MS.gene95541:CDS
AGCGGTGAAAAGGTAGACCC+AGG 0.651820 4.3:-51074333 None:intergenic
TGATCATCAACACGATCATG+TGG 0.666329 4.3:-51077052 None:intergenic
CTGAGAATGGTGGAGTACTG+GGG 0.687268 4.3:+51070524 MS.gene95541:CDS
ATGGTGGAGAGTTAGTGCAA+AGG 0.698402 4.3:+51070755 MS.gene95541:CDS
GGATGTCAATGGAGAAAGTG+CGG 0.714843 4.3:+51071123 MS.gene95541:CDS
GAATGTTCCCACTAGTGATG+AGG 0.716273 4.3:+51077093 MS.gene95541:CDS
ACACAAGGTTCACAACACCT+GGG 0.722910 4.3:+51074316 MS.gene95541:CDS
CCTTGTTTCGACGATCCGAG+CGG 0.734178 4.3:-51074351 None:intergenic
ATCGACGATGAAAAGCATCG+AGG 0.779533 4.3:+51070879 MS.gene95541:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.3 gene 51070370 51077287 51070370 ID=MS.gene95541
chr4.3 mRNA 51070370 51077287 51070370 ID=MS.gene95541.t1;Parent=MS.gene95541
chr4.3 exon 51070370 51071014 51070370 ID=MS.gene95541.t1.exon1;Parent=MS.gene95541.t1
chr4.3 CDS 51070370 51071014 51070370 ID=cds.MS.gene95541.t1;Parent=MS.gene95541.t1
chr4.3 exon 51071114 51071227 51071114 ID=MS.gene95541.t1.exon2;Parent=MS.gene95541.t1
chr4.3 CDS 51071114 51071227 51071114 ID=cds.MS.gene95541.t1;Parent=MS.gene95541.t1
chr4.3 exon 51072256 51072397 51072256 ID=MS.gene95541.t1.exon3;Parent=MS.gene95541.t1
chr4.3 CDS 51072256 51072397 51072256 ID=cds.MS.gene95541.t1;Parent=MS.gene95541.t1
chr4.3 exon 51072593 51072752 51072593 ID=MS.gene95541.t1.exon4;Parent=MS.gene95541.t1
chr4.3 CDS 51072593 51072752 51072593 ID=cds.MS.gene95541.t1;Parent=MS.gene95541.t1
chr4.3 exon 51074274 51074372 51074274 ID=MS.gene95541.t1.exon5;Parent=MS.gene95541.t1
chr4.3 CDS 51074274 51074372 51074274 ID=cds.MS.gene95541.t1;Parent=MS.gene95541.t1
chr4.3 exon 51076149 51076250 51076149 ID=MS.gene95541.t1.exon6;Parent=MS.gene95541.t1
chr4.3 CDS 51076149 51076250 51076149 ID=cds.MS.gene95541.t1;Parent=MS.gene95541.t1
chr4.3 exon 51076838 51076943 51076838 ID=MS.gene95541.t1.exon7;Parent=MS.gene95541.t1
chr4.3 CDS 51076838 51076943 51076838 ID=cds.MS.gene95541.t1;Parent=MS.gene95541.t1
chr4.3 exon 51077019 51077182 51077019 ID=MS.gene95541.t1.exon8;Parent=MS.gene95541.t1
chr4.3 CDS 51077019 51077182 51077019 ID=cds.MS.gene95541.t1;Parent=MS.gene95541.t1
chr4.3 exon 51077272 51077287 51077272 ID=MS.gene95541.t1.exon9;Parent=MS.gene95541.t1
chr4.3 CDS 51077272 51077287 51077272 ID=cds.MS.gene95541.t1;Parent=MS.gene95541.t1
Gene Sequence

>MS.gene95541

ATGGAGAACTGTAGATTAAAAATGATGTATGATCAGCTTAGAATAGATTACGATTACATGCGGATGCGTTTTGAGAAAATGATGCAAGACCATAATGTTAAGGAAGTTAAAGGAAAAGAAGTATTTGATGGAAACTTTAAAGGGAAAAAGAGAACTGAGAATGGTGGAGTACTGGGGCCAATTAAATTTATGGATCTTGGATTGGCTAGTAATAAGGGTAAGGAAGTTAAAGGACAAGAAGTTTCTGATGGAAAATTTATAGACAAAAAGAAAATGAATAATGATGGAGAATTAGTGAAAAGGAAATTTGTTGATGCTGGATTGGATACTAATAAGGTTAAAGAAGTGTTTAATGGAAAATGTGAAAAGAAAAAGAGAACTGAGAATGGTGGAGAGTTAGTGCAAAGGCAATGTAGGGATTTTGTGTTGATTACTAATGCTGAGACTGCGATGGATCATGAAGCTTCTTCCTCATCAATGAGAAAACCTAGAAGTAAGGATCAGTTAGGATCGACGATGAAAAGCATCGAGGTTGCTTCGAAGGAGTTAGTTTTAAGTAAGAATGGGGATGTTAATGTTGATAATGTTGAGGCTACATTGACAAAAGCAAGAGTTACTATTAGAGCTAGATCAGAAGAAACCATGGTAAGTATGATAAATTGTATATTAACTTTCAACTTTGCAAGTTTTATATAATAAATTGTTGTCCTTTATCGCTGAAAGAATTTATTTTTATTGCTTTAGATCACTGATGGATGTCAATGGAGAAAGTGCGGACATAAATTGTCGAAAGGAAACCCGTGTCCTAAAGCTTATTATCGCTGCAGCACATCCCATGGTTGCTCAGTCCGAAAACAGGTAACTTTCATCTGTAACTATCCAAATTACATGTATTTTACTTATTAAGGTCTTAAATTTATGTCACCCTAATGATTGCTTTCACATTATTATATTCAATATTATAGAGAATTACAGTTAAATATGATTGACTATAATATGGTTTTAGTCCCTACAAATATGCCTCGTTTTGGTTTTAGTCCCTCTAAAAAAAAATTGTTTTTGGTCCCTGCAAAATTTTTTGTTTTTTAAAATAGTCCCTGACCCCAGTTTTGTGATGATTTGCATACATGGCACATGATGACTGAACCCATTTTGTAGTTTTTGGTCCCTGCAAAATATTTTGTTTTTTAAAAAGGTCCCTGCAAAATTTTTTTGTTTTTGAAAATAGTCCCTGGAGGGACTATTTTCAAAAACAAAAAATTTTGCAGGGATCAAAAACAAATTTTTTTTACAGGGACTAAAACCAAAACGAGACATATTTGCAGGGACTAAAACCATATTTTAGCCAATATGATTTATGCAACAACAATTGCATTAAAGTCACAACGACATTAAACTCTCTAATTGTTGCCTAGATCTAAGTCACAAACATATTTTAAAACCTTAGTCTTTAATCATTATCTTTATGTATGTTTTCAATTAATTACAAAATAACTTTACACACACAAAAATTATTAGTAATGATTAATTTGATATGCATTGAACGATGTTGGATAACAATAGAATTTAATTGAAATGTGTTTCCACTTTCAAAGCTCATGTTTCTATTCTAGAAAATGGATTTGGATTTGTTTCATTCCGCAATCCATGCATTTACACAGCCAACACGTCAATGTCCATTAAAAATCAAAATATGTATTGAAGTTTGGATTGTCCAATCTTCGATACAGAAACCATCGATGTACTTACTATGTGAATGCATGGATTTGCTGACTGTAACAAACTCAAATCCGAAATGGTAGATATCAAGATAGGTTAATTAAAATACTTATGAAGTGGTATGTACTGTAATTACAATATTATTTAATACAATTCCAACTTAACAGGTACAAAGGTGTGATCTTGATAGAACAGTTGTCAACACAACATATGAAGAAAACCACAACCACTTGTTACCTGCAGCAGCCAAAGAAATGGCAAAAACAACTTCCGCAGCTGCAAAGATGCTTCTTTCAGCATCAACATCAAGTAATGATGGAAAATTGAATGAAAACTCACTCACAAGAACACTCTTTCCTTGTTTTTCAAGTATTGCAACTATACCAACTTCTGCTCCATTTACAACAATTACACTTGATTATACTCAATCTCCAAATACTCCACAAAGGAACCAAGATCAGTTTCAAACTCCTTTAATCACTTAGAGTTCTGCAAATTCCTCAGCTTCTATAATCCCTCAAATACCTAATCAGAACCAATCAAAATTTTCTGGACTACAAATGTCAAACGATGCAGCTAGCCCTTCACAACTATTGGCAATACCAAATATTGTTCAGATAGTTAATGCTGCCATTGCTGCTAATCCAAATTTTCCTGCAGATTTGTAAGCTTCTCTTACTTCCATTATTGGTGATACTCAGCTAAATAACACTCTTGCTGATAATAATAACGACAACCATGACACCGAAGGAAATAGCAATGGTAGCAACAATAATGTCGCAGAAGACAATAGAAATGGAAACATCACATGTAGCAACAACAATGATGGGAAGAAATAAAACCAGGAATACCAATCACAAGAGCTAAACTTCAATTTTTTGGCTCAAAGATTTAGTTGATTTTGTTGCCAATTTATTTTTGTTCATCTGTTATTTTATTTTGGATTTAGAAATTACTCATATTTTTCATAGATACTAGTTTTTTTCTTGGGATGGAGCTTCGATGTTAGTGGTTATATGATCCCCTTAGAGGTATTCTAAAATTTTGTGCCAAAACAACAAAGTATTTTTATTTACTCGGATTGTTTCTCCTGTTTTTTTTTGTTATATATTTTGATGTTGGAATATTTTATTTCTCTAATAATATATATGAAGATTTTTTCTAATCACACTTCATATAAAAAAGGGTTAATTAAGTTTTTAGTCCCTATAAATATTCACAGTTTTGTTTTTAGTCCCTACAAAATAAAATCACACTTTTTAGTCCCTGTCACATTTTCCTTAAGCATTTTAGGGACCAAAAAAGTTGATGGAAATTTTTGACAGGGACTAAAAATGCTGATGGAAAATTTTATAGGGACTAAAAAGTGTGATTTTATTTTAAAGGGACTAAAAACAAAATTCTGAATATTTACAGGGACCATTTACTTAATTAACCCTATAAAAAAAGGGCAATTTCCACCCATGCATGTATGTGGGAAAGAAGTGAAGATTTTGAATAAAGCATGAAAAAGATTTTTTTCTTTTTGAAAAAACTTTATATGAACAAGGGCAATTTTAGTATTTACATATAATTTTAATTAAATTTGTGCGTAACCGACTATTTTTGGGGTGTGGTTAGGAAAAGTCTAATATAAAGTGCGAATTTTTTAAATTGCTTGTGTATTAGTATGATTTTCTTACTTGATTTGTTTTTTCAAATGACATTGTTGGCCTCCACTTACAATAAACTTTTTTTTCGGCTCCTCTAAGGTAGGGTCTTACTTTTCTAGAACCTCCAGAGGCGGAGGGTCCTAGTGGATTCGTGAGGCGATACACGTGTGTTTTGTGATGTCAATGTGGAGGTGTCTGCACACTTGTTTGTCACTTGTCCTTTTTCCTAACAAGTTTGGTATGGTGTCACTAGGTAGTTAGGATTGGAGGCTGGTCTCTTTGAGGTCTTCCATAGTCTTGGTGTTGGGAGGAGGGTCGGGTCAGGTTTGTTGTTGGTTTGATATTTTGTTGTGTGGTGTTGGGTATTGGGCTCCCTTCCTATGTGGAGAAAAGCCCAAATATGTTAGCCCATTATGTCTCACTTATTAGGGGTTAAGAAGAGAGTGTTTTTGTGAGAAACATAGCCACCAAAAAGAGAGAAAAGAAGAGAGAAAGCAATTCCCGTTTTTCTGAAATCTGTCTCAGTAAAACAGCGATTTCTCCCTCATTACTGGTCGGATCGGGCTGATTTTTGGACACAAGGTTCACAACACCTGGGTCTACCTTTTCACCGCTCGGATCGTCGAAACAAGGTCTGAGTTAGGAGAAAACTGTCACGCACTAAAGCAGTTTTTTTTTTGGGTATTTTAATCTTTTTTTGTTCTTTTTTGTACGCTTTGTTGCTTTGTTGTTTTGGCTAGTTGTCGGCACTGTTTGTGCATCACTTTTGAGACTCTCTTGTACCCATATTTGATTATAGTGGAGCTATTTCATTGGTCTGAACGACTCGTGGTTTTTACCCTTACATTGAGGGGTTTTCCACCTTAAAATATCGGTGTTCACTTGCGTTTTTATTTACCTTTCACTGCTGCCATTTATTTGCTGTTGCTCCTCATAGATTCTTGCAAGTATGGGGAATTTTATTTTCGCTGTGCATATTGGTGTGTTACCATATTTATGTGTTAATTTCCCATCAGAGTGGTATCAGAGCTCTTGGTTAAAGGGGCTATTTTACTTGTTTGAATGATGGAGGCAAATACAAATATAACGATGGTTTGTTTGAATGGTTCTAATTATCATTTGTGGAAGGGTAAGATGAAAGATTTGCTATTTGTCAAGAAATGGCATTTACCTGTTTTTGGTTCGAAAAAGCCGGATGGTACTTCTGACGAAGACTGGGAGTTTAAGCATCAACATGTGTGTGGTTTTATTCGACAATATGTTGAAGATAATGTTTATAATCATATTGCTAACGAGACAAATGCAAAAGCTTTGTGGGAAAAGATAGAGTAAATGTATGCCTCTAAATTAGGGAATAATAAAATATTCTTGTTCATAAGTTTAAAGTATCAGGAGGGGGCTTCTATTTCAGACAACTTGAGTGAATTTCAAGGGCTCCTTGATCAGATGTCAGGAATGGGTATCAAGTTTGAAGATGAACGCTTTTGGGACTGTTTCTGTTATTATCTTTACCAGAGTCTTGGGAGACATTTCGGGTTTCTATCACTAGTTCAGCTCCTAATGGTGTTGTTTCTTTGGAAACGGCTAAAGGTTGTATTCTTAATGAGGAGATGAGAAGGAAGGCTCAAGGTTCTTCATGTCAATCTGAGGTACTTGTCACTGAAAATAGGGGGAGAAGTCAGAAAAAGGAACATAAGGGTGGTAGAGAGAATAGTAGAAGCAAGTCCAAGCTGCGAAACAAGAATGTAGAGTGTCATTTTTGTCACAAAATTGGACACATACAAAAGAATTGTTATCAATGGAAAAAGGAGAATAAAGGCAAGAAGGGTAAACAGAAACAAAGGGATCGTGAAGATAATGATGACTGTGTTTCTACTGCTGGTGATGATCTTGTTATTCTTCGTGACCATGAATCAGTTAATCTTGTATCAGATGAGAGCATGTGGATAGTTGATAGTGGTGCTACTTTGCATGTTACACCGAGGAAGGAGTTCTTCACATCTTATACTTCTGGTGATTTTGGAGTGTTGAAGATGGGTAATGATGGTGTGTCTAAGGTAATTGGTGTTGGTGATGTTTGCTTGCAAACCAACATGGGAATGCAATTGTTGCTCAAAGGTGTCAAACATGCTCCAGATGTCCGCTTTAATTTGATATCTGTGCGTATGCTTGATGATTGTGGTTATGATAATCACTTTAGTTGTGGAAAGTGGAATCTCAGCAAAGGTAACTTGGTTGTGGCTAGAGGGGAGAAACTTTCTAAGTTGTATTGGACAAAAGCTTTAGTTGCTAAAGACCATGTGAATGTTGTCCATATGGAAGCATCTTTGTGGCACCGAAGGCTTAGTCATATCAGTGAAAAAAGGCTGAATGTTTTAGCTAAAAAGGATGTACTTCCAGGATTGAAGAATGCAGATTTGGAGAAATGTTCTCATTGCATGGCTGGTAAGCAGACCAGAGTATCATTCAAAAGACATCCTCCCTCAAGGAAGTCAGAATTGCTTCAATTGGTGCATTATGATGTTTGTGGCCCTTTGAAGGTAAAATCCTTTAGTGGTGCACTTTATTTTGTTACCTTTATTGATGATTGTTCCAGAAAACTATGGGTTTATGCCTTGAAGACAAAAGACCAAGTGTTGGAGAAGTTCAAAGAATTTCATGCTTTGGTTGAGAGGCAGACAGGCAAGAAGCTGAAATGTGTTCGTACTGACAATGGTGGTGAGTATTATGGATCATTTGATGTCTATTGCAAGCATCATGGTATTGCACATGAAAAGACTCCTTCTAAAACTCCTCAACTGAATGGTTTATCAGAGAGGATGAATCGAACACTGATTGAGAAGGTGAGGTGTATGCTCTCTGGAGCTAAGTTGCCTAAACACTATTGGGGTGAGGCATTGTACACGGCGGTGCATGTTCTTAATCTCACTCCTACTATTTCTTTGAATGGTGAAGTTCCGGACAATGTTTGGTTTGGAAAGAATGTCAAATATGATCATTTGAGAGTCTTTGGGTGTAAGGCTTTTGTGCATATTCCAAAGGATGAAAGATTCAAGTTGGATGCAAATTCAAAACAATGTATTTTTATCGGCTATAGGCATGATGAGTTTGGTTACAGGTTGTATGATCCTGTAGAGAAAAGGCTTATCAGAAGTCGTGACGTAGTGTTCATGGAAGACCAAACTATTGAAGACATTGATAAGGTGAAGAAGACTACAACAAAGAAAGATGACAATTCGTCTGATATTGATCCAGCTCGATTTCCTATTGATAATTAGGATACTATTGATGATGATATTCAGAATGGTGAGCCACATGATCGTGTTGATGATCAACAACTTAGAGATAATGTGAATGTTCCCACTAGTGATGAGGAGGAGTATATGTCACATGATGAAGATATTGGTGATGCTCCTGAATCATCTCAAGTTCAACTTAGAAGGTCCAACAGACAAAGACAACCATCTACAAGATATGATTCTAATGAGTATGTGACCTTGACTGATGATGGTGAACCTGAGTGTTTTCAAGAGGCAATGGAAAGTGA
Protein sequence

>MS.gene95541.t1

MENCRLKMMYDQLRIDYDYMRMRFEKMMQDHNVKEVKGKEVFDGNFKGKKRTENGGVLGPIKFMDLGLASNKGKEVKGQEVSDGKFIDKKKMNNDGELVKRKFVDAGLDTNKVKEVFNGKCEKKKRTENGGELVQRQCRDFVLITNAETAMDHEASSSSMRKPRSKDQLGSTMKSIEVASKELVLSKNGDVNVDNVEATLTKARVTIRARSEETMITDGCQWRKCGHKLSKGNPCPKAYYRCSTSHGCSVRKQVQRCDLDRTVVNTTYEENHNHLLPAAAKEMAKTTSAAAKMLLSASTSTSIIPQIPNQNQSKFSGLQMSNDAASPSQLLAIPNIVQIVNAAIAANPNFPADFDFSLITGRIGLIFGHKVHNTWVYLFTARIVETRMQIWRNVLIAWLVSRPEYHSKDILPQGSQNCFNWHDEFGYRLYDPVEKRLIRSRDVVFMEDQTIEDIDKDTIDDDIQNGEPHDRVDDQQLRDNVNVPTSDEEEYMSHDEDIGDAPESSQVQLRRGNGK