AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95601

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95601.t1 MTR_7g068600 93.697 238 15 0 1 238 1 238 1.67e-168 464
MS.gene95601.t1 MTR_7g088070 55.303 132 53 2 13 141 50 178 8.03e-44 149
MS.gene95601.t1 MTR_7g088070 55.814 129 53 2 13 141 50 174 2.46e-43 147
MS.gene95601.t1 MTR_2g027860 49.682 157 71 5 13 166 49 200 3.45e-43 149
MS.gene95601.t1 MTR_1g053835 50.000 138 63 4 2 138 246 378 5.36e-36 132
MS.gene95601.t1 MTR_7g098250 50.000 144 57 4 1 138 250 384 5.70e-35 130
MS.gene95601.t1 MTR_7g093030 47.287 129 64 2 13 141 212 336 4.00e-34 126
MS.gene95601.t1 MTR_1g080330 43.226 155 80 5 13 166 175 322 3.63e-32 121
MS.gene95601.t1 MTR_2g086450 42.282 149 63 3 13 141 45 190 1.82e-29 114
MS.gene95601.t1 MTR_6g444980 45.802 131 65 2 13 140 35 162 5.30e-29 112
MS.gene95601.t1 MTR_6g444980 45.455 132 66 2 13 141 35 163 1.13e-28 111
MS.gene95601.t1 MTR_6g032990 42.953 149 66 3 9 141 38 183 1.29e-28 111
MS.gene95601.t1 MTR_5g027440 41.958 143 64 3 13 141 25 162 2.08e-28 108
MS.gene95601.t1 MTR_7g115530 42.636 129 71 1 13 141 218 343 4.29e-28 110
MS.gene95601.t1 MTR_4g081710 39.310 145 72 4 9 141 13 153 2.50e-26 104
MS.gene95601.t1 MTR_1g080330 48.214 112 53 3 13 123 175 282 3.98e-25 100
MS.gene95601.t1 MTR_5g041350 38.095 147 72 4 9 141 11 152 8.23e-25 100
MS.gene95601.t1 MTR_1g053800 43.478 138 72 3 3 138 165 298 1.53e-23 97.1
MS.gene95601.t1 MTR_2g027800 34.884 172 89 4 9 176 35 187 1.79e-23 96.3
MS.gene95601.t1 MTR_2g027800 32.778 180 96 3 9 176 35 201 6.29e-23 95.1
MS.gene95601.t1 MTR_7g115530 42.857 112 61 1 13 124 218 326 2.91e-22 93.6
MS.gene95601.t1 MTR_7g093030 59.459 74 26 2 13 86 212 281 3.80e-22 92.8
MS.gene95601.t1 MTR_1g090670 35.256 156 79 3 13 159 44 186 2.16e-20 87.8
MS.gene95601.t1 MTR_7g115530 56.923 65 25 1 13 77 218 279 3.03e-18 82.0
MS.gene95601.t1 MTR_7g115530 56.923 65 25 1 13 77 218 279 8.64e-18 81.6
MS.gene95601.t1 MTR_1g053830 48.101 79 37 2 9 87 14 88 1.58e-16 72.8
MS.gene95601.t1 MTR_7g093010 36.111 144 78 4 1 138 201 336 2.36e-16 77.4
MS.gene95601.t1 MTR_7g115530 58.182 55 20 1 13 67 218 269 3.37e-14 70.5
MS.gene95601.t1 MTR_3g450310 34.839 155 89 6 2 149 5 154 1.07e-13 68.2
MS.gene95601.t1 MTR_7g115530 55.357 56 22 1 13 68 218 270 1.48e-13 68.9
MS.gene95601.t1 MTR_2g023580 61.818 55 18 1 15 69 57 108 3.93e-13 67.0
MS.gene95601.t1 MTR_2g084230 46.512 86 38 2 15 95 99 181 3.93e-13 68.2
MS.gene95601.t1 MTR_2g084230 46.512 86 38 2 15 95 99 181 4.03e-13 67.8
MS.gene95601.t1 MTR_8g086410 57.627 59 22 1 15 73 113 168 8.04e-13 67.4
MS.gene95601.t1 MTR_8g086410 57.627 59 22 1 15 73 111 166 8.55e-13 67.4
MS.gene95601.t1 MTR_7g069660 55.000 60 24 1 15 74 73 129 1.12e-12 67.0
MS.gene95601.t1 MTR_2g016220 57.627 59 22 1 15 73 91 146 2.07e-12 66.2
MS.gene95601.t1 MTR_2g016220 57.627 59 22 1 15 73 91 146 2.09e-12 66.2
MS.gene95601.t1 MTR_6g071625 53.448 58 24 1 15 72 204 258 3.00e-12 65.5
MS.gene95601.t1 MTR_8g066310 52.542 59 25 1 15 73 175 230 6.44e-12 64.7
MS.gene95601.t1 MTR_1g021520 53.448 58 24 1 15 72 128 182 1.04e-11 63.5
MS.gene95601.t1 MTR_3g064840 39.773 88 50 1 15 102 276 360 1.32e-11 63.9
MS.gene95601.t1 MTR_5g029470 53.448 58 24 1 15 72 221 275 2.81e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95601.t1 AT2G01060 47.904 167 81 4 9 172 11 174 2.64e-46 155
MS.gene95601.t1 AT5G06800 49.275 138 66 2 9 146 188 321 5.16e-38 136
MS.gene95601.t1 AT5G06800 47.887 142 67 2 9 146 188 326 1.06e-37 135
MS.gene95601.t1 AT4G28610 48.529 136 56 3 9 138 221 348 4.68e-33 124
MS.gene95601.t1 AT1G79430 42.857 154 69 4 13 161 34 173 1.43e-31 119
MS.gene95601.t1 AT5G29000 43.571 140 73 3 2 138 177 313 1.45e-31 119
MS.gene95601.t1 AT5G29000 43.571 140 73 3 2 138 177 313 1.45e-31 119
MS.gene95601.t1 AT5G29000 43.571 140 73 3 2 138 220 356 2.12e-31 119
MS.gene95601.t1 AT3G24120 38.690 168 79 3 13 168 41 196 1.25e-30 115
MS.gene95601.t1 AT1G69580 43.046 151 64 4 9 141 30 176 2.01e-30 115
MS.gene95601.t1 AT1G69580 42.763 152 64 4 9 141 30 177 3.45e-30 115
MS.gene95601.t1 AT3G04450 35.795 176 90 4 9 170 194 360 1.79e-29 114
MS.gene95601.t1 AT3G04450 35.795 176 90 4 9 170 194 360 1.79e-29 114
MS.gene95601.t1 AT4G13640 39.412 170 75 4 13 168 37 192 2.42e-29 111
MS.gene95601.t1 AT3G04450 35.795 176 90 4 9 170 234 400 3.22e-29 114
MS.gene95601.t1 AT3G04030 43.624 149 64 4 9 141 41 185 5.04e-29 112
MS.gene95601.t1 AT5G18240 45.517 145 59 5 13 141 45 185 6.12e-29 112
MS.gene95601.t1 AT5G18240 45.517 145 59 5 13 141 45 185 6.12e-29 112
MS.gene95601.t1 AT3G13040 43.537 147 74 3 1 141 228 371 6.87e-29 113
MS.gene95601.t1 AT3G13040 43.537 147 74 3 1 141 228 371 6.87e-29 113
MS.gene95601.t1 AT3G13040 43.537 147 74 3 1 141 228 371 6.87e-29 113
MS.gene95601.t1 AT3G24120 38.012 171 79 4 13 168 41 199 1.31e-28 110
MS.gene95601.t1 AT4G13640 38.728 173 75 5 13 168 37 195 2.21e-27 106
MS.gene95601.t1 AT2G01060 44.800 125 66 3 51 172 1 125 2.37e-27 105
MS.gene95601.t1 AT2G20400 44.094 127 67 2 13 138 231 354 2.95e-27 108
MS.gene95601.t1 AT3G04030 42.953 149 64 5 9 141 41 184 1.98e-26 105
MS.gene95601.t1 AT2G20400 42.063 126 70 1 13 138 231 353 4.20e-26 105
MS.gene95601.t1 AT5G18240 44.138 145 59 6 13 141 45 183 1.00e-25 103
MS.gene95601.t1 AT5G45580 38.194 144 68 3 13 138 7 147 4.62e-25 99.8
MS.gene95601.t1 AT5G45580 38.194 144 68 3 13 138 23 163 6.49e-25 99.8
MS.gene95601.t1 AT3G04030 41.611 149 61 5 9 141 41 179 1.47e-24 100
MS.gene95601.t1 AT5G18240 43.448 145 56 6 13 141 45 179 1.97e-24 100
MS.gene95601.t1 AT5G18240 43.448 145 56 6 13 141 45 179 1.97e-24 100
MS.gene95601.t1 AT3G12730 37.778 135 73 3 13 139 23 154 4.16e-23 94.0
MS.gene95601.t1 AT5G06800 62.319 69 23 1 9 77 188 253 9.75e-23 94.0
MS.gene95601.t1 AT5G29000 46.809 94 47 1 2 95 220 310 1.42e-19 85.9
MS.gene95601.t1 AT3G04450 54.412 68 28 1 9 76 234 298 1.53e-18 83.2
MS.gene95601.t1 AT1G79430 37.815 119 58 3 48 161 1 108 3.09e-17 79.3
MS.gene95601.t1 AT2G42660 61.905 63 21 1 15 77 52 111 8.56e-16 74.7
MS.gene95601.t1 AT2G06020 50.820 61 27 1 13 73 86 143 1.04e-15 75.1
MS.gene95601.t1 AT3G04030 40.000 110 49 3 48 141 1 109 1.09e-14 72.4
MS.gene95601.t1 AT2G40260 60.345 58 20 1 15 72 84 138 2.20e-14 72.0
MS.gene95601.t1 AT4G04605 51.351 74 32 2 15 88 16 85 2.14e-13 67.4
MS.gene95601.t1 AT5G42630 50.000 74 33 2 15 87 107 177 2.68e-13 67.4
MS.gene95601.t1 AT5G42630 50.000 74 33 2 15 87 107 177 3.41e-13 67.8
MS.gene95601.t1 AT4G17695 38.835 103 47 2 15 104 166 265 8.61e-12 64.3
MS.gene95601.t1 AT5G16560 46.575 73 34 2 15 85 221 290 1.42e-11 63.9
MS.gene95601.t1 AT2G38300 55.932 59 23 1 15 73 56 111 1.94e-11 63.2
MS.gene95601.t1 AT2G02060 60.000 55 19 1 15 69 31 82 2.12e-11 62.4
MS.gene95601.t1 AT2G02060 60.000 55 19 1 15 69 31 82 2.12e-11 62.4
MS.gene95601.t1 AT1G14600 55.932 59 23 1 15 73 25 80 2.38e-11 62.4
MS.gene95601.t1 AT1G32240 53.448 58 24 1 15 72 215 269 2.43e-11 63.2
MS.gene95601.t1 AT1G32240 53.448 58 24 1 15 72 215 269 2.74e-11 62.8
MS.gene95601.t1 AT4G37180 51.724 58 25 1 13 70 210 264 8.52e-11 61.2
MS.gene95601.t1 AT4G37180 51.724 58 25 1 13 70 217 271 8.84e-11 61.2

Find 60 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTACTTGTGGTGGATTC+TGG 0.193661 7.4:+35062774 None:intergenic
GGTGCCTAGTTTCTTATTAT+AGG 0.220382 7.4:-35061708 None:intergenic
ACCAAACCTGGAACCTTTCT+TGG 0.254138 7.4:-35061766 MS.gene95601:CDS
TACGATATAGAACAGAAATT+TGG 0.266828 7.4:+35062623 None:intergenic
GGTGGATTCTGGAATCAATT+TGG 0.287077 7.4:+35062785 None:intergenic
TGGAGGCTGTAAATAGGCTT+GGG 0.332347 7.4:-35064760 MS.gene95601:CDS
TCTTTGTGGAGGCTGTAAAT+AGG 0.333114 7.4:-35064766 MS.gene95601:CDS
TCCAAGAAAGGTTCCAGGTT+TGG 0.364366 7.4:+35061765 None:intergenic
GCACCTCAGCCTGCATTTGA+AGG 0.377972 7.4:+35062501 None:intergenic
TTGGATATGTTCAGAAATAC+AGG 0.386624 7.4:-35062812 MS.gene95601:intron
ACACGTCAAAAGCCACTTGC+AGG 0.391889 7.4:-35063602 MS.gene95601:intron
GTGGAGGCTGTAAATAGGCT+TGG 0.397815 7.4:-35064761 MS.gene95601:CDS
AAGAAAAGCTTGCAAGCCTT+TGG 0.401704 7.4:-35061951 MS.gene95601:CDS
CTGACTCCAAGAAAGGTTCC+AGG 0.408019 7.4:+35061760 None:intergenic
GGAAAGTACCTAGACAGAAT+TGG 0.410201 7.4:-35062001 MS.gene95601:CDS
TGCTGTTTGATGTTCTTCCT+CGG 0.424128 7.4:+35061868 None:intergenic
GAACCTTTCTTGGAGTCAGC+TGG 0.440714 7.4:-35061756 MS.gene95601:CDS
ATTCCTTTCAGTATACCTCT+TGG 0.449845 7.4:+35063658 None:intergenic
CAGAATTCAGCAACCAAACC+TGG 0.457761 7.4:-35061778 MS.gene95601:CDS
GTTGCTGAATTCTGAGGTAG+TGG 0.463218 7.4:+35061787 None:intergenic
AACTAGGCACCAAAGGTGAT+TGG 0.478600 7.4:+35061720 None:intergenic
CAGCCTCCACAAAGAGATCA+TGG 0.480797 7.4:+35064774 None:intergenic
CAGCCAGCTGACTCCAAGAA+AGG 0.496169 7.4:+35061753 None:intergenic
AGCAACCTACCAATCACCTT+TGG 0.500347 7.4:-35061729 MS.gene95601:CDS
ACAACTCCATGATCTCTTTG+TGG 0.503279 7.4:-35064780 MS.gene95601:CDS
ACTGAAAGGAATGAAGGCTA+TGG 0.503485 7.4:-35063647 MS.gene95601:CDS
TACTTTGTCCAATTCTGTCT+AGG 0.510514 7.4:+35061993 None:intergenic
CTGAAAGGAATGAAGGCTAT+GGG 0.510802 7.4:-35063646 MS.gene95601:CDS
CACACAAAGAACGCTTAAGA+TGG 0.520735 7.4:-35064808 MS.gene95601:CDS
TCAAACAGCAAGAAAGCAAA+AGG 0.520861 7.4:-35061855 MS.gene95601:CDS
AAAAGAAGTCCTTCAAATGC+AGG 0.534364 7.4:-35062510 MS.gene95601:CDS
ATGGGTTCCACTCGCTCAGA+TGG 0.537642 7.4:-35064839 MS.gene95601:CDS
AGGTATACTGAAAGGAATGA+AGG 0.543020 7.4:-35063653 MS.gene95601:CDS
TTCAGATTCCTCAGTAAGAG+AGG 0.547975 7.4:+35061907 None:intergenic
AAAGCTTGCAAGCCTTTGGT+TGG 0.548496 7.4:-35061947 MS.gene95601:CDS
GAGGCTGTAAATAGGCTTGG+GGG 0.562654 7.4:-35064758 MS.gene95601:CDS
AATAAGAAACTAGGCACCAA+AGG 0.567599 7.4:+35061713 None:intergenic
GATTATTTATGAAGGAGGAA+AGG 0.568981 7.4:-35062675 MS.gene95601:intron
GGAGGCTGTAAATAGGCTTG+GGG 0.579180 7.4:-35064759 MS.gene95601:CDS
AAAACCTATAATAAGAAACT+AGG 0.579659 7.4:+35061704 None:intergenic
TCTTAAGCGTTCTTTGTGTG+TGG 0.591577 7.4:+35064811 None:intergenic
GGTTTGGTTGCTGAATTCTG+AGG 0.596881 7.4:+35061781 None:intergenic
GGTGTTGCTTTACCAACCAA+AGG 0.599644 7.4:+35061935 None:intergenic
AGTCCTTCAAATGCAGGCTG+AGG 0.600265 7.4:-35062504 MS.gene95601:CDS
ACCTCTTCCCTCTCTTACTG+AGG 0.608111 7.4:-35061915 MS.gene95601:CDS
GATCCTAGTTCAAAACCTGT+AGG 0.608319 7.4:+35061812 None:intergenic
ATCCTTTAAAACACAATCCG+AGG 0.610019 7.4:-35061885 MS.gene95601:CDS
GGCAGAGCCATCTGAGCGAG+TGG 0.612768 7.4:+35064832 None:intergenic
AGGCACCAAAGGTGATTGGT+AGG 0.616341 7.4:+35061724 None:intergenic
ACGCATGAGTGATAAAGTTG+AGG 0.616870 7.4:-35062474 MS.gene95601:intron
TCCTCAGTAAGAGAGGGAAG+AGG 0.625723 7.4:+35061914 None:intergenic
TCAGATTCCTCAGTAAGAGA+GGG 0.627051 7.4:+35061908 None:intergenic
ACACCAAGAGGTATACTGAA+AGG 0.630195 7.4:-35063661 MS.gene95601:CDS
TGAAAGGAATGAAGGCTATG+GGG 0.636336 7.4:-35063645 MS.gene95601:CDS
GAAAGGAATGAAGGCTATGG+GGG 0.636670 7.4:-35063644 MS.gene95601:CDS
ACTCCATGATCTCTTTGTGG+AGG 0.654213 7.4:-35064777 MS.gene95601:CDS
TTGTCAGGGGCAACACCAAG+AGG 0.658933 7.4:-35063673 MS.gene95601:intron
GGTGGAGAAGAACCTGCAAG+TGG 0.679561 7.4:+35063590 None:intergenic
AGGCTGAAAATTGAAGCACA+AGG 0.685954 7.4:-35062022 MS.gene95601:CDS
AGGCTGTAAATAGGCTTGGG+GGG 0.689265 7.4:-35064757 MS.gene95601:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTTAATGTAGTTTA+TGG + chr7.4:35063593-35063612 None:intergenic 10.0%
!!! AAACAATATAATTATTTCTA+TGG - chr7.4:35064407-35064426 MS.gene95601:intron 10.0%
!!! ATATAGTCTTTATAATTTTT+AGG - chr7.4:35064347-35064366 MS.gene95601:intron 10.0%
!!! ATTTTTAAATAAAAAAGAGA+AGG + chr7.4:35062701-35062720 None:intergenic 10.0%
!!! TTTTTAAATTATTAGTTCAA+AGG - chr7.4:35062080-35062099 MS.gene95601:intron 10.0%
!! AAAGAAAAGAAAAAAAAACA+GGG + chr7.4:35062562-35062581 None:intergenic 15.0%
!! AATATGCTAATTAGTAAAAA+TGG - chr7.4:35063409-35063428 MS.gene95601:intron 15.0%
!! TACTTTAATATAAGTCAATA+TGG + chr7.4:35063649-35063668 None:intergenic 15.0%
!! TTTAATCAAATACTATTACT+TGG + chr7.4:35063810-35063829 None:intergenic 15.0%
!!! CAAAATTTTGTATTATTTGT+TGG - chr7.4:35064485-35064504 MS.gene95601:intron 15.0%
!!! TATGATATTTTAATTTGTCA+AGG - chr7.4:35064256-35064275 MS.gene95601:intron 15.0%
!! GAAAGAAAAGAAAAAAAAAC+AGG + chr7.4:35062563-35062582 None:intergenic 20.0%
!! TAATATTTAGTTAGAGTTGT+AGG - chr7.4:35062792-35062811 MS.gene95601:CDS 20.0%
!! TATTTGAATATTGTTTGTCA+GGG - chr7.4:35062861-35062880 MS.gene95601:intron 20.0%
!! TCTCAAAAAAACTATAATCT+CGG - chr7.4:35063478-35063497 MS.gene95601:intron 20.0%
!! TTAATATAAGTCAATATGGA+TGG + chr7.4:35063645-35063664 None:intergenic 20.0%
!! TTCGTAATTCATATAAATGT+GGG + chr7.4:35063390-35063409 None:intergenic 20.0%
!! TTTAAATAAAAAAGAGAAGG+AGG + chr7.4:35062698-35062717 None:intergenic 20.0%
!! TTTATAGTATTTGAACCTAT+AGG - chr7.4:35064100-35064119 MS.gene95601:intron 20.0%
!! TTTCGTAATTCATATAAATG+TGG + chr7.4:35063391-35063410 None:intergenic 20.0%
!!! AAGAAATTTTCATATTTGGA+AGG - chr7.4:35062262-35062281 MS.gene95601:intron 20.0%
!!! AATTTTACGACCAATATAAA+TGG + chr7.4:35062983-35063002 None:intergenic 20.0%
!!! ACTTGTATTTCAATTTTCAA+GGG + chr7.4:35063685-35063704 None:intergenic 20.0%
!!! AGAATTGTAGAAAGTTTTAA+AGG - chr7.4:35064373-35064392 MS.gene95601:intron 20.0%
!!! ATTTTACGACCAATATAAAT+GGG + chr7.4:35062982-35063001 None:intergenic 20.0%
!!! ATTTTGTATTATTTGTTGGA+AGG - chr7.4:35064489-35064508 MS.gene95601:intron 20.0%
!!! GAATTGTAGAAAGTTTTAAA+GGG - chr7.4:35064374-35064393 MS.gene95601:intron 20.0%
!!! GTCTAAGAAATTTTCATATT+TGG - chr7.4:35062258-35062277 MS.gene95601:intron 20.0%
!!! TACTTGTATTTCAATTTTCA+AGG + chr7.4:35063686-35063705 None:intergenic 20.0%
!!! TGATGGATAAAATGTTATTT+TGG - chr7.4:35063243-35063262 MS.gene95601:intron 20.0%
!!! TGATTTGTAAAACTGTTTTA+AGG + chr7.4:35063309-35063328 None:intergenic 20.0%
!!! TTATCTTATACGATTTTTGA+TGG - chr7.4:35063226-35063245 MS.gene95601:intron 20.0%
! ACAAAAAATTACACTAACAG+AGG + chr7.4:35062346-35062365 None:intergenic 25.0%
! ACAACACATGAAAAATTTGT+TGG - chr7.4:35063190-35063209 MS.gene95601:intron 25.0%
! AGTCGTGAATGAATAATTTA+CGG - chr7.4:35061956-35061975 MS.gene95601:CDS 25.0%
! ATTTGAATATTGTTTGTCAG+GGG - chr7.4:35062862-35062881 MS.gene95601:intron 25.0%
! CTATTTGAATATTGTTTGTC+AGG - chr7.4:35062860-35062879 MS.gene95601:intron 25.0%
! CTCAAAAAAACTATAATCTC+GGG - chr7.4:35063479-35063498 MS.gene95601:intron 25.0%
! CTTATTTGAATTGAGATAGA+TGG - chr7.4:35062300-35062319 MS.gene95601:intron 25.0%
! GATTGTTGAATAAGATTTGA+TGG - chr7.4:35062416-35062435 MS.gene95601:intron 25.0%
! GTAAATGTAGAAGACATATT+TGG + chr7.4:35064315-35064334 None:intergenic 25.0%
! GTTATAATTTCACTTGAAGT+TGG + chr7.4:35063844-35063863 None:intergenic 25.0%
! TACGATATAGAACAGAAATT+TGG + chr7.4:35063928-35063947 None:intergenic 25.0%
! TGTTCCATGATTATTTATGA+AGG - chr7.4:35063865-35063884 MS.gene95601:intron 25.0%
! TTCAAAGGAAATATGTCAAA+TGG - chr7.4:35062095-35062114 MS.gene95601:intron 25.0%
!! CAAGTATTTAGAGTAGTTTA+TGG - chr7.4:35063052-35063071 MS.gene95601:intron 25.0%
!! TTTTTCTTAAGTCAAGTCAA+TGG + chr7.4:35062607-35062626 None:intergenic 25.0%
!!! TTGTCATCAATTTTGATTGT+CGG + chr7.4:35062509-35062528 None:intergenic 25.0%
AAAAAACTATAATCTCGGGT+TGG - chr7.4:35063483-35063502 MS.gene95601:intron 30.0%
AAAATGGTTGAACTATGATC+CGG - chr7.4:35062028-35062047 MS.gene95601:CDS 30.0%
AATGACTAATATGCTCATCT+TGG - chr7.4:35063717-35063736 MS.gene95601:intron 30.0%
ATCTACAAATTGCACTAAAC+AGG + chr7.4:35061862-35061881 None:intergenic 30.0%
CCATGATTTAAAAACCGAAT+TGG - chr7.4:35061917-35061936 MS.gene95601:CDS 30.0%
CTAAACCAAAAACTTACTTG+TGG + chr7.4:35063787-35063806 None:intergenic 30.0%
CTTTAATGACTACATCCTAT+AGG + chr7.4:35064118-35064137 None:intergenic 30.0%
GATTATTTATGAAGGAGGAA+AGG - chr7.4:35063873-35063892 MS.gene95601:intron 30.0%
GATTTAAAAACCGAATTGGA+CGG - chr7.4:35061921-35061940 MS.gene95601:CDS 30.0%
TATTTAGTTAGAGTTGTAGG+AGG - chr7.4:35062795-35062814 MS.gene95601:CDS 30.0%
TCCATGATTATTTATGAAGG+AGG - chr7.4:35063868-35063887 MS.gene95601:intron 30.0%
TCCTCCTTCATAAATAATCA+TGG + chr7.4:35063872-35063891 None:intergenic 30.0%
TTATTTGAACAGTTTGATCG+TGG - chr7.4:35061984-35062003 MS.gene95601:CDS 30.0%
TTGGATATGTTCAGAAATAC+AGG - chr7.4:35063736-35063755 MS.gene95601:intron 30.0%
! ATTTTAGACAACTTGTACGT+GGG - chr7.4:35063267-35063286 MS.gene95601:intron 30.0%
! CCAATTCGGTTTTTAAATCA+TGG + chr7.4:35061920-35061939 None:intergenic 30.0%
! GTCATGCAAAGTATTATTAG+TGG + chr7.4:35061837-35061856 None:intergenic 30.0%
!! GTTTGGTTTGCTAAAAAACA+AGG + chr7.4:35063364-35063383 None:intergenic 30.0%
!! TACCAAAGGGTACTAAAAAA+GGG - chr7.4:35062128-35062147 MS.gene95601:intron 30.0%
!!! ATTTTAAAGTAGAGTTGTCG+AGG + chr7.4:35062454-35062473 None:intergenic 30.0%
AAAAGAAGTCCTTCAAATGC+AGG - chr7.4:35064038-35064057 MS.gene95601:intron 35.0%
AAACCAAGCTCTTTGATACT+TGG - chr7.4:35062631-35062650 MS.gene95601:CDS 35.0%
AACCAAAAACTTACTTGTGG+TGG + chr7.4:35063784-35063803 None:intergenic 35.0%
AATAAGAAACTAGGCACCAA+AGG + chr7.4:35064838-35064857 None:intergenic 35.0%
ACAAGAAAACTCCAGTTGTA+CGG + chr7.4:35063336-35063355 None:intergenic 35.0%
AGACAAAACAGAACAACACA+AGG - chr7.4:35063163-35063182 MS.gene95601:intron 35.0%
AGGTATACTGAAAGGAATGA+AGG - chr7.4:35062895-35062914 MS.gene95601:intron 35.0%
ATTCCTTTCAGTATACCTCT+TGG + chr7.4:35062893-35062912 None:intergenic 35.0%
CATATAAATGTGGGTGTGTT+TGG + chr7.4:35063381-35063400 None:intergenic 35.0%
GAACATGTATAACTTCCAAG+AGG - chr7.4:35064431-35064450 MS.gene95601:intron 35.0%
TACACTAACAGAGGTTTATG+TGG + chr7.4:35062337-35062356 None:intergenic 35.0%
TACTTTGTCCAATTCTGTCT+AGG + chr7.4:35064558-35064577 None:intergenic 35.0%
TCAAACAGCAAGAAAGCAAA+AGG - chr7.4:35064693-35064712 MS.gene95601:intron 35.0%
TCCAGATCAAACGGTTAAAA+TGG - chr7.4:35062012-35062031 MS.gene95601:CDS 35.0%
TCTTCTCCACCCATTTATAT+TGG - chr7.4:35062970-35062989 MS.gene95601:intron 35.0%
TGACAACGTAAATGCATGAT+TGG - chr7.4:35062523-35062542 MS.gene95601:CDS 35.0%
TGGCAAAAAAACTCACTATC+TGG + chr7.4:35061817-35061836 None:intergenic 35.0%
TTACGACCAATATAAATGGG+TGG + chr7.4:35062979-35062998 None:intergenic 35.0%
TTATTCATTCACGACTCAAC+TGG + chr7.4:35061952-35061971 None:intergenic 35.0%
! ATCCACCACAAGTAAGTTTT+TGG - chr7.4:35063779-35063798 MS.gene95601:intron 35.0%
! CATTTTAGACAACTTGTACG+TGG - chr7.4:35063266-35063285 MS.gene95601:intron 35.0%
! GATGAGAATGTTTTTCCTAC+AGG - chr7.4:35064721-35064740 MS.gene95601:intron 35.0%
! GTTTGCTAAAAAACAAGGAC+AGG + chr7.4:35063359-35063378 None:intergenic 35.0%
!! ACCATTTTAACCGTTTGATC+TGG + chr7.4:35062016-35062035 None:intergenic 35.0%
!! ATCCTTTAAAACACAATCCG+AGG - chr7.4:35064663-35064682 MS.gene95601:intron 35.0%
!! CTACCAAAGGGTACTAAAAA+AGG - chr7.4:35062127-35062146 MS.gene95601:intron 35.0%
!! TTCCTCGGATTGTGTTTTAA+AGG + chr7.4:35064668-35064687 None:intergenic 35.0%
!! TTTTAGTACCCTTTGGTAGT+AGG + chr7.4:35062126-35062145 None:intergenic 35.0%
!!! AACCCTTTTTTAGTACCCTT+TGG + chr7.4:35062133-35062152 None:intergenic 35.0%
!!! TTTCCTACAGGTTTTGAACT+AGG - chr7.4:35064733-35064752 MS.gene95601:intron 35.0%
AAGAAAAGCTTGCAAGCCTT+TGG - chr7.4:35064597-35064616 MS.gene95601:intron 40.0%
ACAACTCCATGATCTCTTTG+TGG - chr7.4:35061768-35061787 MS.gene95601:CDS 40.0%
ACGCATGAGTGATAAAGTTG+AGG - chr7.4:35064074-35064093 MS.gene95601:intron 40.0%
ACTGAAAGGAATGAAGGCTA+TGG - chr7.4:35062901-35062920 MS.gene95601:intron 40.0%
AGGCTGAAAATTGAAGCACA+AGG - chr7.4:35064526-35064545 MS.gene95601:intron 40.0%
AGTCCAAGTATCAAAGAGCT+TGG + chr7.4:35062637-35062656 None:intergenic 40.0%
ATCCGCAAAACAGTAAGTAC+TGG - chr7.4:35063127-35063146 MS.gene95601:intron 40.0%
ATGGTCAATCCTACTACCAA+AGG - chr7.4:35062114-35062133 MS.gene95601:intron 40.0%
CAAATCAAACACCGTACAAC+TGG - chr7.4:35063322-35063341 MS.gene95601:intron 40.0%
CACACAAAGAACGCTTAAGA+TGG - chr7.4:35061740-35061759 MS.gene95601:CDS 40.0%
CCAAGAGGAAAAAACAGACA+TGG - chr7.4:35064446-35064465 MS.gene95601:intron 40.0%
CTGAAAGGAATGAAGGCTAT+GGG - chr7.4:35062902-35062921 MS.gene95601:intron 40.0%
GATCCTAGTTCAAAACCTGT+AGG + chr7.4:35064739-35064758 None:intergenic 40.0%
GGAAGGTTCAAAAAAGCTTG+AGG - chr7.4:35064506-35064525 MS.gene95601:intron 40.0%
GTGGTTATGTCCAGATCAAA+CGG - chr7.4:35062003-35062022 MS.gene95601:CDS 40.0%
TCAGATTCCTCAGTAAGAGA+GGG + chr7.4:35064643-35064662 None:intergenic 40.0%
TCTTAAGCGTTCTTTGTGTG+TGG + chr7.4:35061740-35061759 None:intergenic 40.0%
TCTTTGTGGAGGCTGTAAAT+AGG - chr7.4:35061782-35061801 MS.gene95601:CDS 40.0%
TGAAAGGAATGAAGGCTATG+GGG - chr7.4:35062903-35062922 MS.gene95601:intron 40.0%
TGGTCAATCCTACTACCAAA+GGG - chr7.4:35062115-35062134 MS.gene95601:intron 40.0%
TTCAGATTCCTCAGTAAGAG+AGG + chr7.4:35064644-35064663 None:intergenic 40.0%
! ACACCAAGAGGTATACTGAA+AGG - chr7.4:35062887-35062906 MS.gene95601:intron 40.0%
! GTCCAGTACTTACTGTTTTG+CGG + chr7.4:35063132-35063151 None:intergenic 40.0%
! TGCTGTTTGATGTTCTTCCT+CGG + chr7.4:35064683-35064702 None:intergenic 40.0%
!! AACTTACTTGTGGTGGATTC+TGG + chr7.4:35063777-35063796 None:intergenic 40.0%
!! GGAAAGTACCTAGACAGAAT+TGG - chr7.4:35064547-35064566 MS.gene95601:intron 40.0%
!! GGTGGATTCTGGAATCAATT+TGG + chr7.4:35063766-35063785 None:intergenic 40.0%
!!! CCATGTCTGTTTTTTCCTCT+TGG + chr7.4:35064449-35064468 None:intergenic 40.0%
AAAGCTTGCAAGCCTTTGGT+TGG - chr7.4:35064601-35064620 MS.gene95601:intron 45.0%
AACTAGGCACCAAAGGTGAT+TGG + chr7.4:35064831-35064850 None:intergenic 45.0%
ACCAAACCTGGAACCTTTCT+TGG - chr7.4:35064782-35064801 MS.gene95601:CDS 45.0%
ACTCCATGATCTCTTTGTGG+AGG - chr7.4:35061771-35061790 MS.gene95601:CDS 45.0%
ACTGGTTGAACCGTCCAATT+CGG + chr7.4:35061934-35061953 None:intergenic 45.0%
AGCAACCTACCAATCACCTT+TGG - chr7.4:35064819-35064838 MS.gene95601:CDS 45.0%
CAGAATTCAGCAACCAAACC+TGG - chr7.4:35064770-35064789 MS.gene95601:CDS 45.0%
CATTGAGCCTACAAGACATC+CGG + chr7.4:35062050-35062069 None:intergenic 45.0%
CTATGATCCGGATGTCTTGT+AGG - chr7.4:35062040-35062059 MS.gene95601:CDS 45.0%
GAAAGGAATGAAGGCTATGG+GGG - chr7.4:35062904-35062923 MS.gene95601:intron 45.0%
TCCAAGAAAGGTTCCAGGTT+TGG + chr7.4:35064786-35064805 None:intergenic 45.0%
TGGAGGCTGTAAATAGGCTT+GGG - chr7.4:35061788-35061807 MS.gene95601:CDS 45.0%
TGTCTTGTAGGCTCAATGTC+TGG - chr7.4:35062052-35062071 MS.gene95601:intron 45.0%
! GGTTTGGTTGCTGAATTCTG+AGG + chr7.4:35064770-35064789 None:intergenic 45.0%
!! GGTGTTGCTTTACCAACCAA+AGG + chr7.4:35064616-35064635 None:intergenic 45.0%
!! GTTGCTGAATTCTGAGGTAG+TGG + chr7.4:35064764-35064783 None:intergenic 45.0%
ACACGTCAAAAGCCACTTGC+AGG - chr7.4:35062946-35062965 MS.gene95601:intron 50.0%
ACCTCTTCCCTCTCTTACTG+AGG - chr7.4:35064633-35064652 MS.gene95601:intron 50.0%
AGGCACCAAAGGTGATTGGT+AGG + chr7.4:35064827-35064846 None:intergenic 50.0%
AGGCTGTAAATAGGCTTGGG+GGG - chr7.4:35061791-35061810 MS.gene95601:CDS 50.0%
AGTCCTTCAAATGCAGGCTG+AGG - chr7.4:35064044-35064063 MS.gene95601:intron 50.0%
CAGCCTCCACAAAGAGATCA+TGG + chr7.4:35061777-35061796 None:intergenic 50.0%
CTGACTCCAAGAAAGGTTCC+AGG + chr7.4:35064791-35064810 None:intergenic 50.0%
GAACCTTTCTTGGAGTCAGC+TGG - chr7.4:35064792-35064811 MS.gene95601:CDS 50.0%
GAGGCTGTAAATAGGCTTGG+GGG - chr7.4:35061790-35061809 MS.gene95601:CDS 50.0%
GGAGGCTGTAAATAGGCTTG+GGG - chr7.4:35061789-35061808 MS.gene95601:CDS 50.0%
GTGGAGGCTGTAAATAGGCT+TGG - chr7.4:35061787-35061806 MS.gene95601:CDS 50.0%
TCCTCAGTAAGAGAGGGAAG+AGG + chr7.4:35064637-35064656 None:intergenic 50.0%
ATGGGTTCCACTCGCTCAGA+TGG - chr7.4:35061709-35061728 MS.gene95601:CDS 55.0%
GCACCTCAGCCTGCATTTGA+AGG + chr7.4:35064050-35064069 None:intergenic 55.0%
GGTGGAGAAGAACCTGCAAG+TGG + chr7.4:35062961-35062980 None:intergenic 55.0%
TTGTCAGGGGCAACACCAAG+AGG - chr7.4:35062875-35062894 MS.gene95601:intron 55.0%
! CAGCCAGCTGACTCCAAGAA+AGG + chr7.4:35064798-35064817 None:intergenic 55.0%
GGCAGAGCCATCTGAGCGAG+TGG + chr7.4:35061719-35061738 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 35061709 35064861 35061709 ID=MS.gene95601
chr7.4 mRNA 35061709 35064861 35061709 ID=MS.gene95601.t1;Parent=MS.gene95601
chr7.4 exon 35064750 35064861 35064750 ID=MS.gene95601.t1.exon1;Parent=MS.gene95601.t1
chr7.4 CDS 35064750 35064861 35064750 ID=cds.MS.gene95601.t1;Parent=MS.gene95601.t1
chr7.4 exon 35063603 35063688 35063603 ID=MS.gene95601.t1.exon2;Parent=MS.gene95601.t1
chr7.4 CDS 35063603 35063688 35063603 ID=cds.MS.gene95601.t1;Parent=MS.gene95601.t1
chr7.4 exon 35062781 35062820 35062781 ID=MS.gene95601.t1.exon3;Parent=MS.gene95601.t1
chr7.4 CDS 35062781 35062820 35062781 ID=cds.MS.gene95601.t1;Parent=MS.gene95601.t1
chr7.4 exon 35062626 35062683 35062626 ID=MS.gene95601.t1.exon4;Parent=MS.gene95601.t1
chr7.4 CDS 35062626 35062683 35062626 ID=cds.MS.gene95601.t1;Parent=MS.gene95601.t1
chr7.4 exon 35062475 35062544 35062475 ID=MS.gene95601.t1.exon5;Parent=MS.gene95601.t1
chr7.4 CDS 35062475 35062544 35062475 ID=cds.MS.gene95601.t1;Parent=MS.gene95601.t1
chr7.4 exon 35061709 35062059 35061709 ID=MS.gene95601.t1.exon6;Parent=MS.gene95601.t1
chr7.4 CDS 35061709 35062059 35061709 ID=cds.MS.gene95601.t1;Parent=MS.gene95601.t1
Gene Sequence

>MS.gene95601

ATGGGTTCCACTCGCTCAGATGGCTCTGCCACACACAAAGAACGCTTAAGATGGACACAACAACTCCATGATCTCTTTGTGGAGGCTGTAAATAGGCTTGGGGGGCCAGATAGTGAGTTTTTTTGCCACTAATAATACTTTGCATGACAACCTGTTTAGTGCAATTTGTAGATTTTTTGTTAACGTGCTAAGTTTCTTTAGTTCACAGCCATGATTTAAAAACCGAATTGGACGGTTCAACCAGTTGAGTCGTGAATGAATAATTTACGGTTTGATTATTTGAACAGTTTGATCGTGGTTATGTCCAGATCAAACGGTTAAAATGGTTGAACTATGATCCGGATGTCTTGTAGGCTCAATGTCTGGTTCAGTTTTTAAATTATTAGTTCAAAGGAAATATGTCAAATGGTCAATCCTACTACCAAAGGGTACTAAAAAAGGGTTAATGAAGAGATGTTTGTTGATTTGTGTTTGCAATATTTACAATGACAGAATTTATGATTTCTTCAAAAAAAAAAAAAAATGATAGAATTTATGAACTTTTTCTTTGTCTAAGAAATTTTCATATTTGGAAGGCAACTTAAAAGTTCACTTATTTGAATTGAGATAGATGGACTTACACAAACCACATAAACCTCTGTTAGTGTAATTTTTTGTACTGAAATTGTTGCAATTAAGATAATCATGTTAAGCAGTCAAGAGATCTTGATTGTTGAATAAGATTTGATGGCTCATATTCTGACCTCGACAACTCTACTTTAAAATGAAACTTGAAATGTAAGTTGTCTGATTTTCATCCGACAATCAAAATTGATGACAACGTAAATGCATGATTGGATATAATTCATTTCCCTGTTTTTTTTTCTTTTCTTTCTTTCTATTCACATGACTCAAACCATTGACTTGACTTAAGAAAAATTAAAAACCAAGCTCTTTGATACTTGGACTAACAATTTGATTTATAAACTTATTTGTAGTTGCATTTTCCTCCTTCTCTTTTTTATTTAAAAATGATTTTGTGTGCTACTGATATATCTATTTTGACTTTTTTACGTTAACTTTTAGCTTGAAAGTATAATATGATAATATTTAGTTAGAGTTGTAGGAGGCAACATTATTTACATAGAAAAGTTTATGATTATATGCATTTTCTATTTGAATATTGTTTGTCAGGGGCAACACCAAGAGGTATACTGAAAGGAATGAAGGCTATGGGGGTTTCAGAATTGAAAATATTACACGTCAAAAGCCACTTGCAGGTTCTTCTCCACCCATTTATATTGGTCGTAAAATTTTGAATGAATTTATCACATGATCTAAAGTATTAAGTATAAACACTTGTACAAGTATTTAGAGTAGTTTATGGAAATAAATTATCATATGACTTTTTTTTTTTTTATTATATAAACTTGTGTTTTATCCGCAAAACAGTAAGTACTGGACAAAACATTACAAGACAAAACAGAACAACACAAGGTAGAACAACACATGAAAAATTTGTTGGTATTGAAATTTTTTTATCTTATACGATTTTTGATGGATAAAATGTTATTTTGGCATTTTAGACAACTTGTACGTGGGAAAAAAAAATTCATATCCTTAAAACAGTTTTACAAATCAAACACCGTACAACTGGAGTTTTCTTGTCCTGTCCTTGTTTTTTAGCAAACCAAACACACCCACATTTATATGAATTACGAAATATGCTAATTAGTAAAAATGGAAGAAAATATTAAAAGCTACAAGTTCAAACTATATTTAAAATAGCTTCTCAAAAAAACTATAATCTCGGGTTGGAAGCTTGTTTATCAAACACATATCATTTGATCAAACAAGTTTATAAGTTATTTCAATAAGCTATAAGTTGTTTGTACAAACTCTCCATAAACTACATTAAATTATTTATTAGTGTTTTGACACATTTATACTTTTACCATCCATATTGACTTATATTAAAGTAAAGCTAAATAATTCCCTTGAAAATTGAAATACAAGTAACGTTATAAGCAATGACTAATATGCTCATCTTGGATATGTTCAGAAATACAGGATTTCCAAATTGATTCCAGAATCCACCACAAGTAAGTTTTTGGTTTAGCCAAGTAATAGTATTTGATTAAACAATGATAAGACCAACTTCAAGTGAAATTATAACTTGTTCCATGATTATTTATGAAGGAGGAAAGGTTGAGAAGAGAAGCATATCAGATATGCTGCCAAATTTCTGTTCTATATCGTAAGTCAACAACTAATTAAGCAATAATTTTCATATCTCATTTTCAAACAACTTATATAATTATATTTTGTAAATGAACAGTGCTCTTCAGCTAAAAGAAGTCCTTCAAATGCAGGCTGAGGTGCAAAAACGCATGAGTGATAAAGTTGAGGTATTTTATAGTATTTGAACCTATAGGATGTAGTCATTAAAGTTTGATTTATCTAATTTTCTTTTTAGTAAACTTTCACACAAAAATTTAACAGTTTGTTGAAGAAAAATAACTTAAAAGTTTCAATATTATGTTTTTTTTTTTTTTTATCAAGTAAGCATATGATATTTTAATTTGTCAAGGAAGATCAAACATATTACACTTTTGCTTCTTTATCCAAATATGTCTTCTACATTTACAATAATATATATATATAGTCTTTATAATTTTTAGGTCAAGAATTGTAGAAAGTTTTAAAGGGAATTAAAACAAAACAATATAATTATTTCTATGGAGAACATGTATAACTTCCAAGAGGAAAAAACAGACATGGAGATAATCTCTAAAGTCAAAATTTTGTATTATTTGTTGGAAGGTTCAAAAAAGCTTGAGGCTGAAAATTGAAGCACAAGGAAAGTACCTAGACAGAATTGGACAAAGTAATCAAATCAGAACTATTACAAGAAAAGCTTGCAAGCCTTTGGTTGGTAAAGCAACACCTCTTCCCTCTCTTACTGAGGAATCTGAATCCTTTAAAACACAATCCGAGGAAGAACATCAAACAGCAAGAAAGCAAAAGGTCACAGATGAGAATGTTTTTCCTACAGGTTTTGAACTAGGATCATCCACTACCTCAGAATTCAGCAACCAAACCTGGAACCTTTCTTGGAGTCAGCTGGCTGAAGCAACCTACCAATCACCTTTGGTGCCTAGTTTCTTATTATAG
Protein sequence

>MS.gene95601.t1

MGSTRSDGSATHKERLRWTQQLHDLFVEAVNRLGGPDRATPRGILKGMKAMGVSELKILHVKSHLQKYRISKLIPESTTRGKVEKRSISDMLPNFCSISALQLKEVLQMQAEVQKRMSDKVEVQKSLRLKIEAQGKYLDRIGQSNQIRTITRKACKPLVGKATPLPSLTEESESFKTQSEEEHQTARKQKVTDENVFPTGFELGSSTTSEFSNQTWNLSWSQLAEATYQSPLVPSFLL