AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95656

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95656.t1 MTR_7g069660 92.938 354 19 2 1 350 1 352 0.0 644
MS.gene95656.t1 MTR_2g084230 62.069 116 40 2 18 132 50 162 1.73e-37 137
MS.gene95656.t1 MTR_2g084230 62.069 116 40 2 18 132 50 162 3.69e-37 137
MS.gene95656.t1 MTR_8g086410 57.983 119 43 2 21 132 58 176 5.35e-37 138
MS.gene95656.t1 MTR_8g086410 57.983 119 43 2 21 132 56 174 5.51e-37 138
MS.gene95656.t1 MTR_2g023580 81.159 69 13 0 55 123 43 111 7.57e-35 128
MS.gene95656.t1 MTR_8g086410 54.762 126 43 3 21 132 58 183 1.89e-34 131
MS.gene95656.t1 MTR_2g016220 71.951 82 23 0 50 131 72 153 1.00e-33 128
MS.gene95656.t1 MTR_2g016220 71.951 82 23 0 50 131 72 153 1.03e-33 128
MS.gene95656.t1 MTR_2g016220 68.605 86 23 1 50 131 72 157 1.25e-31 122
MS.gene95656.t1 MTR_1g112370 72.727 66 18 0 58 123 13 78 2.30e-28 113
MS.gene95656.t1 MTR_0223s0040 65.278 72 25 0 52 123 3 74 5.75e-28 110
MS.gene95656.t1 MTR_6g071625 55.294 85 31 1 66 150 201 278 3.81e-23 99.4
MS.gene95656.t1 MTR_8g066310 44.144 111 58 1 35 141 137 247 1.48e-22 97.4
MS.gene95656.t1 MTR_1g021520 59.211 76 29 1 66 141 125 198 2.77e-22 95.5
MS.gene95656.t1 MTR_5g029470 56.098 82 35 1 61 141 212 293 5.83e-22 96.3
MS.gene95656.t1 MTR_3g450310 42.105 114 56 3 49 162 1 104 1.01e-21 92.0
MS.gene95656.t1 MTR_3g064840 64.179 67 23 1 61 126 267 333 3.01e-21 94.7
MS.gene95656.t1 MTR_7g098250 64.286 56 20 0 68 123 263 318 6.73e-19 87.8
MS.gene95656.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 1.16e-18 85.5
MS.gene95656.t1 MTR_1g080330 62.500 56 21 0 68 123 176 231 1.93e-18 84.7
MS.gene95656.t1 MTR_7g093030 49.367 79 39 1 63 140 208 286 2.02e-18 84.7
MS.gene95656.t1 MTR_6g032990 66.038 53 18 0 68 120 43 95 2.11e-18 85.9
MS.gene95656.t1 MTR_1g080330 62.500 56 21 0 68 123 176 231 2.31e-18 85.9
MS.gene95656.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 2.37e-18 85.1
MS.gene95656.t1 MTR_2g086450 62.500 56 21 0 68 123 46 101 3.10e-18 85.5
MS.gene95656.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 3.10e-18 85.9
MS.gene95656.t1 MTR_5g027440 62.069 58 22 0 63 120 21 78 3.59e-18 83.6
MS.gene95656.t1 MTR_7g093030 49.367 79 39 1 63 140 208 286 5.44e-18 84.7
MS.gene95656.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 5.52e-18 84.3
MS.gene95656.t1 MTR_7g088070 55.224 67 30 0 59 125 42 108 1.47e-17 82.0
MS.gene95656.t1 MTR_7g088070 55.224 67 30 0 59 125 42 108 1.68e-17 81.6
MS.gene95656.t1 MTR_1g090670 60.714 56 22 0 68 123 45 100 8.10e-17 80.1
MS.gene95656.t1 MTR_2g027800 60.714 56 22 0 68 123 40 95 9.58e-17 80.1
MS.gene95656.t1 MTR_7g115530 63.462 52 19 0 68 119 219 270 1.06e-16 79.7
MS.gene95656.t1 MTR_7g115530 60.714 56 22 0 68 123 219 274 1.29e-16 79.3
MS.gene95656.t1 MTR_2g027800 62.264 53 20 0 68 120 40 92 1.44e-16 79.3
MS.gene95656.t1 MTR_5g041350 56.604 53 23 0 68 120 16 68 1.81e-16 79.7
MS.gene95656.t1 MTR_2g027860 61.404 57 22 0 69 125 51 107 2.33e-16 79.3
MS.gene95656.t1 MTR_1g053830 56.364 55 24 0 69 123 20 74 2.46e-16 73.9
MS.gene95656.t1 MTR_1g053835 52.055 73 34 1 69 140 260 332 3.17e-16 79.7
MS.gene95656.t1 MTR_6g444980 55.357 56 25 0 68 123 36 91 3.56e-15 76.3
MS.gene95656.t1 MTR_6g444980 55.357 56 25 0 68 123 36 91 3.99e-15 75.9
MS.gene95656.t1 MTR_4g081710 55.357 56 25 0 68 123 18 73 5.29e-15 75.5
MS.gene95656.t1 MTR_7g068600 56.667 60 23 1 69 125 15 74 4.15e-13 68.6
MS.gene95656.t1 MTR_4g021790 51.786 56 26 1 65 120 166 220 1.38e-12 67.4
MS.gene95656.t1 MTR_1g053800 54.717 53 24 0 69 121 179 231 1.82e-12 67.8
MS.gene95656.t1 MTR_4g086835 45.205 73 31 1 57 120 255 327 3.48e-12 67.4
MS.gene95656.t1 MTR_4g064730 48.684 76 37 2 46 120 109 183 8.81e-12 65.5
MS.gene95656.t1 MTR_2g450070 39.316 117 57 6 66 178 212 318 1.20e-11 66.2
MS.gene95656.t1 MTR_4g113140 55.769 52 23 0 69 120 237 288 1.24e-11 65.9
MS.gene95656.t1 MTR_2g450070 39.316 117 57 6 66 178 212 318 1.29e-11 66.2
MS.gene95656.t1 MTR_7g093010 49.231 65 33 0 69 133 215 279 2.41e-11 64.7
MS.gene95656.t1 MTR_4g086835 45.205 73 30 2 57 120 255 326 7.97e-11 63.2
MS.gene95656.t1 MTR_5g054300 55.102 49 22 0 72 120 203 251 9.99e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95656.t1 AT2G38300 42.025 326 153 14 44 350 32 340 1.25e-57 190
MS.gene95656.t1 AT2G40260 63.889 108 37 1 50 157 65 170 4.11e-39 143
MS.gene95656.t1 AT2G42660 78.788 66 14 0 59 124 42 107 1.92e-31 119
MS.gene95656.t1 AT2G02060 65.432 81 28 0 40 120 2 82 1.62e-29 114
MS.gene95656.t1 AT2G02060 65.432 81 28 0 40 120 2 82 1.62e-29 114
MS.gene95656.t1 AT4G04605 59.302 86 33 1 55 138 2 87 1.45e-28 110
MS.gene95656.t1 AT1G14600 68.657 67 21 0 59 125 15 81 2.03e-28 111
MS.gene95656.t1 AT4G04580 58.209 67 28 0 57 123 4 70 3.67e-23 94.7
MS.gene95656.t1 AT5G42630 67.213 61 20 0 66 126 104 164 2.20e-22 95.5
MS.gene95656.t1 AT5G42630 67.213 61 20 0 66 126 104 164 2.48e-22 94.4
MS.gene95656.t1 AT1G32240 54.878 82 36 1 61 141 206 287 9.61e-22 95.5
MS.gene95656.t1 AT1G32240 54.878 82 36 1 61 141 206 287 1.25e-21 95.1
MS.gene95656.t1 AT4G04555 60.000 65 26 0 59 123 6 70 1.64e-21 88.6
MS.gene95656.t1 AT5G16560 58.108 74 30 1 61 133 212 285 5.72e-21 93.6
MS.gene95656.t1 AT4G17695 62.295 61 23 0 66 126 163 223 3.98e-20 90.1
MS.gene95656.t1 AT5G45580 62.069 58 22 0 63 120 3 60 2.00e-18 84.3
MS.gene95656.t1 AT5G45580 62.069 58 22 0 63 120 19 76 2.57e-18 84.3
MS.gene95656.t1 AT3G04030 58.929 56 23 0 68 123 46 101 1.73e-17 83.2
MS.gene95656.t1 AT3G04030 58.929 56 23 0 68 123 46 101 2.04e-17 83.2
MS.gene95656.t1 AT3G04450 49.333 75 30 1 57 123 220 294 2.30e-17 82.0
MS.gene95656.t1 AT3G04450 49.333 75 30 1 57 123 180 254 2.48e-17 82.8
MS.gene95656.t1 AT3G04450 49.333 75 30 1 57 123 180 254 2.48e-17 82.8
MS.gene95656.t1 AT3G04450 49.333 75 30 1 57 123 220 294 2.69e-17 83.2
MS.gene95656.t1 AT5G29000 63.636 55 20 0 69 123 190 244 3.17e-17 82.4
MS.gene95656.t1 AT5G29000 63.636 55 20 0 69 123 190 244 3.17e-17 82.4
MS.gene95656.t1 AT4G28610 61.818 55 21 0 69 123 227 281 3.55e-17 82.4
MS.gene95656.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.56e-17 82.4
MS.gene95656.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.56e-17 82.4
MS.gene95656.t1 AT5G29000 63.636 55 20 0 69 123 233 287 3.65e-17 81.6
MS.gene95656.t1 AT5G18240 58.929 56 23 0 68 123 46 101 4.12e-17 82.4
MS.gene95656.t1 AT5G18240 58.929 56 23 0 68 123 46 101 4.12e-17 82.4
MS.gene95656.t1 AT5G18240 58.929 56 23 0 68 123 46 101 4.12e-17 82.4
MS.gene95656.t1 AT5G29000 63.636 55 20 0 69 123 233 287 4.29e-17 82.4
MS.gene95656.t1 AT2G01060 61.404 57 22 0 69 125 17 73 9.84e-17 79.7
MS.gene95656.t1 AT3G12730 57.143 56 24 0 68 123 24 79 1.54e-16 78.2
MS.gene95656.t1 AT4G13640 56.604 53 23 0 68 120 38 90 5.90e-16 77.8
MS.gene95656.t1 AT4G13640 56.604 53 23 0 68 120 38 90 6.28e-16 77.8
MS.gene95656.t1 AT3G13040 59.649 57 23 0 69 125 243 299 6.93e-16 79.0
MS.gene95656.t1 AT3G13040 59.649 57 23 0 69 125 243 299 6.93e-16 79.0
MS.gene95656.t1 AT3G13040 59.649 57 23 0 69 125 243 299 6.93e-16 79.0
MS.gene95656.t1 AT3G24120 56.604 53 23 0 68 120 42 94 7.40e-16 77.4
MS.gene95656.t1 AT3G24120 56.604 53 23 0 68 120 42 94 7.73e-16 77.4
MS.gene95656.t1 AT2G20400 57.895 57 24 0 69 125 233 289 2.03e-15 77.0
MS.gene95656.t1 AT1G79430 55.357 56 25 0 68 123 35 90 2.06e-15 77.0
MS.gene95656.t1 AT2G20400 57.895 57 24 0 69 125 233 289 2.09e-15 77.0
MS.gene95656.t1 AT5G06800 54.098 61 28 0 69 129 194 254 3.35e-15 75.5
MS.gene95656.t1 AT3G04030 57.143 56 23 1 68 123 46 100 5.67e-15 75.9
MS.gene95656.t1 AT1G69580 55.357 56 25 0 68 123 35 90 6.98e-15 75.1
MS.gene95656.t1 AT1G69580 55.357 56 25 0 68 123 35 90 7.37e-15 75.1
MS.gene95656.t1 AT5G06800 54.098 61 28 0 69 129 194 254 7.99e-15 75.5
MS.gene95656.t1 AT5G06800 54.098 61 28 0 69 129 194 254 8.53e-15 75.1
MS.gene95656.t1 AT2G06020 36.522 115 62 3 15 120 27 139 2.58e-14 73.2
MS.gene95656.t1 AT5G59570 46.591 88 43 3 35 120 108 193 1.17e-13 71.2
MS.gene95656.t1 AT5G59570 46.591 88 43 3 35 120 108 193 1.17e-13 71.2
MS.gene95656.t1 AT2G40260 58.929 56 21 1 102 157 1 54 1.94e-12 67.4
MS.gene95656.t1 AT1G25550 59.184 49 20 0 72 120 214 262 2.37e-12 67.8
MS.gene95656.t1 AT2G03500 57.143 49 21 0 72 120 238 286 1.08e-11 66.2
MS.gene95656.t1 AT1G25550 46.269 67 32 1 72 138 214 276 1.46e-11 64.7
MS.gene95656.t1 AT3G46640 54.545 55 24 1 66 120 143 196 1.95e-11 64.7
MS.gene95656.t1 AT3G46640 54.545 55 24 1 66 120 143 196 1.95e-11 64.7
MS.gene95656.t1 AT3G46640 54.545 55 24 1 66 120 143 196 2.71e-11 64.3
MS.gene95656.t1 AT3G62670 43.056 72 34 2 53 120 200 268 3.37e-11 64.7

Find 59 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTGAAGAACCAATTT+TGG 0.129269 7.4:-34061492 None:intergenic
TGTTGATAGTTCTCTAAAAT+TGG 0.214021 7.4:-34062579 None:intergenic
CTTCAATTGATGAATATAAA+AGG 0.228877 7.4:+34061504 MS.gene95656:CDS
GATATTGATGTTGCTATTAT+TGG 0.260085 7.4:-34062525 None:intergenic
GAAAGGAGTCAATATTTCTT+TGG 0.270906 7.4:+34062937 MS.gene95656:CDS
GAAAAGAAAATCTTCAAATA+TGG 0.289910 7.4:+34062748 MS.gene95656:CDS
CTATATGATGATGTTTGATT+TGG 0.300830 7.4:-34061919 None:intergenic
GGAAATTATCATTTGGTTCA+TGG 0.310661 7.4:-34062610 None:intergenic
TCATGTGAAGAGTCATTTAC+AGG 0.340032 7.4:+34061539 MS.gene95656:CDS
TGTAGGAGCAACTCCAAAAT+TGG 0.369328 7.4:+34061479 MS.gene95656:intron
TAGAATATGTCTCATTCATA+TGG 0.376004 7.4:-34062966 None:intergenic
GCCTTGAAGCATGGGAAGTT+GGG 0.390047 7.4:-34061890 None:intergenic
ATTAATTGAAACACTTGCCT+TGG 0.400061 7.4:-34060980 None:intergenic
TATCACTAAAATTAAATTCA+AGG 0.404007 7.4:+34062786 MS.gene95656:CDS
GATCATCTCTTAAAGATTCA+TGG 0.404027 7.4:-34062646 None:intergenic
AATGAGAGGAAATTATCATT+TGG 0.404536 7.4:-34062617 None:intergenic
TAAAATTGGATGAATCCATT+TGG 0.405764 7.4:-34062565 None:intergenic
CTTCATGCTGTGCAAAGACT+TGG 0.407098 7.4:+34061180 MS.gene95656:CDS
AGAAGAACAAGATGGTTCTA+AGG 0.407367 7.4:+34062907 MS.gene95656:CDS
ACCCAACTTCCCATGCTTCA+AGG 0.422458 7.4:+34061889 MS.gene95656:CDS
GAATCTAGAGCTGAGTTCTA+TGG 0.429313 7.4:+34062485 MS.gene95656:CDS
CAAATCAAACATCATCATAT+AGG 0.434083 7.4:+34061920 MS.gene95656:CDS
TAACAGTCTTGGCTGATCAT+AGG 0.435782 7.4:+34061827 MS.gene95656:intron
TTTACAGATGTGGATCATAT+GGG 0.447788 7.4:+34062411 MS.gene95656:intron
AATCATCTTATGTTTGCAAA+AGG 0.448039 7.4:+34062879 MS.gene95656:CDS
TAATAGCAACATCAATATCC+AGG 0.451694 7.4:+34062529 MS.gene95656:CDS
TTCAAATATGGATCTTGAGT+TGG 0.452490 7.4:+34062760 MS.gene95656:CDS
GAAATGAGATCTTGTGCTCT+TGG 0.453092 7.4:-34062680 None:intergenic
AGAAAACGAAAATGAAGATC+AGG 0.462194 7.4:-34061159 None:intergenic
CCAACGTAGCCTAGGCAACT+TGG 0.470061 7.4:-34061133 None:intergenic
AGCCTTGAAGCATGGGAAGT+TGG 0.470813 7.4:-34061891 None:intergenic
GGAGTATAGCCTTGAAGCAT+GGG 0.488714 7.4:-34061898 None:intergenic
ATGAGAGAGGAAGAAACATA+TGG 0.490280 7.4:+34060946 MS.gene95656:CDS
CATGCTGTGCAAAGACTTGG+TGG 0.512111 7.4:+34061183 MS.gene95656:CDS
TGGAGTATAGCCTTGAAGCA+TGG 0.512500 7.4:-34061899 None:intergenic
GGCAACTTGGATCTAACATA+AGG 0.518664 7.4:-34061120 None:intergenic
ATGTTAGATCCAAGTTGCCT+AGG 0.523365 7.4:+34061124 MS.gene95656:CDS
GGTTCTAAGGAGCAAGAGAA+AGG 0.533531 7.4:+34062920 MS.gene95656:CDS
AAAGTAGTTGACACAAATCA+AGG 0.545661 7.4:+34061695 MS.gene95656:CDS
TGGTGATCATAAATGTATAG+AGG 0.550152 7.4:+34062814 MS.gene95656:CDS
TTTGGAAAATACTATGACCC+TGG 0.552012 7.4:-34062547 None:intergenic
TCAGGAGTCCAACGTAGCCT+AGG 0.559435 7.4:-34061141 None:intergenic
CCAAGTTGCCTAGGCTACGT+TGG 0.561524 7.4:+34061133 MS.gene95656:CDS
TCTTGCCATGTATGAAAACA+TGG 0.579727 7.4:+34062442 MS.gene95656:CDS
CATAGGCTTCTTGTAGACAA+TGG 0.581433 7.4:+34061844 MS.gene95656:CDS
CTAACATAAGGCCTCACTGA+TGG 0.585574 7.4:-34061108 None:intergenic
GTGCAAAAGAACAGCAATGG+AGG 0.587335 7.4:+34061039 MS.gene95656:CDS
TTACAGATGTGGATCATATG+GGG 0.594097 7.4:+34062412 MS.gene95656:intron
GATGTGCAAAAGAACAGCAA+TGG 0.601073 7.4:+34061036 MS.gene95656:CDS
AAATTAAATTCAAGGAGCAA+TGG 0.605129 7.4:+34062794 MS.gene95656:CDS
TAACTTGCCTGAAAATCAAG+AGG 0.607982 7.4:+34062709 MS.gene95656:CDS
ATTCATGGCCACAAAATGAG+AGG 0.620365 7.4:-34062631 None:intergenic
AATAGCAACATCAATATCCA+GGG 0.624382 7.4:+34062530 MS.gene95656:CDS
GATGATTGTGAAATTAGTGA+AGG 0.638841 7.4:+34061006 MS.gene95656:CDS
ATATGGATCAGAATGTTCCA+AGG 0.643313 7.4:+34060963 MS.gene95656:CDS
TAGCTAGATGAATATGAGAG+AGG 0.659511 7.4:+34060933 None:intergenic
ATGCTGTGCAAAGACTTGGT+GGG 0.679037 7.4:+34061184 MS.gene95656:CDS
AGGATGAAAGAAGAACAAGA+TGG 0.696684 7.4:+34062899 MS.gene95656:CDS
GTGAGAAGAAACCATCAGTG+AGG 0.735513 7.4:+34061097 MS.gene95656:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTAATTTTATTTTT+TGG - chr7.4:34062231-34062250 None:intergenic 0.0%
!! AATAAGTGATTACATAATTA+GGG + chr7.4:34061762-34061781 MS.gene95656:intron 15.0%
!! TAAAAAGTCATAGATAAATA+TGG + chr7.4:34061562-34061581 MS.gene95656:intron 15.0%
!! TATCACTAAAATTAAATTCA+AGG + chr7.4:34062786-34062805 MS.gene95656:CDS 15.0%
!!! TTATTTTTTTTTTACAGATG+TGG + chr7.4:34062401-34062420 MS.gene95656:intron 15.0%
!! CAATAAGTGATTACATAATT+AGG + chr7.4:34061761-34061780 MS.gene95656:intron 20.0%
!! CTTCAATTGATGAATATAAA+AGG + chr7.4:34061504-34061523 MS.gene95656:CDS 20.0%
!! GAAAAATTGAAATCTAGAAA+AGG - chr7.4:34061347-34061366 None:intergenic 20.0%
!! GAAAAGAAAATCTTCAAATA+TGG + chr7.4:34062748-34062767 MS.gene95656:CDS 20.0%
!! TATTATTGTCTTAGAATTGA+AGG + chr7.4:34062376-34062395 MS.gene95656:intron 20.0%
!!! AAGACCTTTTTTTTATTCAT+AGG + chr7.4:34062119-34062138 MS.gene95656:intron 20.0%
!!! AGACCTTTTTTTTATTCATA+GGG + chr7.4:34062120-34062139 MS.gene95656:intron 20.0%
!!! CTAGATTTCAATTTTTCATA+GGG + chr7.4:34061350-34061369 MS.gene95656:intron 20.0%
!!! TAGATTTCAATTTTTCATAG+GGG + chr7.4:34061351-34061370 MS.gene95656:intron 20.0%
!!! TCTAGATTTCAATTTTTCAT+AGG + chr7.4:34061349-34061368 MS.gene95656:intron 20.0%
!!! TTTTAAACGTAAATGACTTA+AGG - chr7.4:34062303-34062322 None:intergenic 20.0%
! AAATTAAATTCAAGGAGCAA+TGG + chr7.4:34062794-34062813 MS.gene95656:CDS 25.0%
! AAGTTTCAAAACAAGTACAA+AGG + chr7.4:34062178-34062197 MS.gene95656:intron 25.0%
! AATCATCTTATGTTTGCAAA+AGG + chr7.4:34062879-34062898 MS.gene95656:CDS 25.0%
! AATGAGAGGAAATTATCATT+TGG - chr7.4:34062620-34062639 None:intergenic 25.0%
! AGTTTCAAAACAAGTACAAA+GGG + chr7.4:34062179-34062198 MS.gene95656:intron 25.0%
! ATACATATATGTTATGTGTG+TGG - chr7.4:34061224-34061243 None:intergenic 25.0%
! CAAATCAAACATCATCATAT+AGG + chr7.4:34061920-34061939 MS.gene95656:CDS 25.0%
! GATCCCTATGAATAAAAAAA+AGG - chr7.4:34062126-34062145 None:intergenic 25.0%
! GTAAATGACTTAAGGAAATT+TGG - chr7.4:34062295-34062314 None:intergenic 25.0%
! TAAAATTGGATGAATCCATT+TGG - chr7.4:34062568-34062587 None:intergenic 25.0%
! TTGTATATATGTAACAGTCT+TGG + chr7.4:34061816-34061835 MS.gene95656:intron 25.0%
!! ATCAATTGAAGAACCAATTT+TGG - chr7.4:34061495-34061514 None:intergenic 25.0%
!! CTATATGATGATGTTTGATT+TGG - chr7.4:34061922-34061941 None:intergenic 25.0%
!! GATATTGATGTTGCTATTAT+TGG - chr7.4:34062528-34062547 None:intergenic 25.0%
!! TTAATGATTTTCTTGTGTGT+AGG + chr7.4:34061462-34061481 MS.gene95656:intron 25.0%
!!! TGTTGATAGTTCTCTAAAAT+TGG - chr7.4:34062582-34062601 None:intergenic 25.0%
AAAGCTAGCTAGCTATATAT+AGG - chr7.4:34061594-34061613 None:intergenic 30.0%
AAAGTAGTTGACACAAATCA+AGG + chr7.4:34061695-34061714 MS.gene95656:CDS 30.0%
AATAGCAACATCAATATCCA+GGG + chr7.4:34062530-34062549 MS.gene95656:CDS 30.0%
ACACAAATCAAGGTACAATA+AGG + chr7.4:34061705-34061724 MS.gene95656:intron 30.0%
AGAAAACGAAAATGAAGATC+AGG - chr7.4:34061162-34061181 None:intergenic 30.0%
ATCTATGTGTTTGATCATCA+TGG - chr7.4:34061310-34061329 None:intergenic 30.0%
ATTAATTGAAACACTTGCCT+TGG - chr7.4:34060983-34061002 None:intergenic 30.0%
ATTCTTATCGAAAAACGAGA+AGG - chr7.4:34062157-34062176 None:intergenic 30.0%
GAAAGGAGTCAATATTTCTT+TGG + chr7.4:34062937-34062956 MS.gene95656:CDS 30.0%
GATCATCTCTTAAAGATTCA+TGG - chr7.4:34062649-34062668 None:intergenic 30.0%
GATGATTGTGAAATTAGTGA+AGG + chr7.4:34061006-34061025 MS.gene95656:CDS 30.0%
GTAACCTAGAATTTACAACA+TGG + chr7.4:34061278-34061297 MS.gene95656:intron 30.0%
TAATAGCAACATCAATATCC+AGG + chr7.4:34062529-34062548 MS.gene95656:CDS 30.0%
TGGTGATCATAAATGTATAG+AGG + chr7.4:34062814-34062833 MS.gene95656:CDS 30.0%
TTCAAATATGGATCTTGAGT+TGG + chr7.4:34062760-34062779 MS.gene95656:CDS 30.0%
TTCAATATCAAGTTAGTCCT+AGG + chr7.4:34061376-34061395 MS.gene95656:intron 30.0%
TTCTTATCGAAAAACGAGAA+GGG - chr7.4:34062156-34062175 None:intergenic 30.0%
TTGAATTGATTAACCTACCT+AGG - chr7.4:34061396-34061415 None:intergenic 30.0%
TTTACAGATGTGGATCATAT+GGG + chr7.4:34062411-34062430 MS.gene95656:intron 30.0%
! AGTAATTTTACAACTGTCGT+TGG - chr7.4:34062357-34062376 None:intergenic 30.0%
! CGTTTAAAATTGTAACCTCA+AGG + chr7.4:34062314-34062333 MS.gene95656:intron 30.0%
! CTTAAGGAAATTTGGAACAA+AGG - chr7.4:34062287-34062306 None:intergenic 30.0%
! GATAATTTCCTCTCATTTTG+TGG + chr7.4:34062623-34062642 MS.gene95656:CDS 30.0%
! GATTTGAAGTTTAAACCTTG+AGG - chr7.4:34062332-34062351 None:intergenic 30.0%
! GGAAATTATCATTTGGTTCA+TGG - chr7.4:34062613-34062632 None:intergenic 30.0%
! TTGTACCATGTTTTCATACA+TGG - chr7.4:34062450-34062469 None:intergenic 30.0%
! TTTTACAGATGTGGATCATA+TGG + chr7.4:34062410-34062429 MS.gene95656:intron 30.0%
AGGATGAAAGAAGAACAAGA+TGG + chr7.4:34062899-34062918 MS.gene95656:CDS 35.0%
ATATCAAGTTAGTCCTAGGT+AGG + chr7.4:34061380-34061399 MS.gene95656:intron 35.0%
ATATGGATCAGAATGTTCCA+AGG + chr7.4:34060963-34060982 MS.gene95656:CDS 35.0%
ATGAGAGAGGAAGAAACATA+TGG + chr7.4:34060946-34060965 MS.gene95656:CDS 35.0%
CACACCATGTTGTAAATTCT+AGG - chr7.4:34061285-34061304 None:intergenic 35.0%
CTAGAATTTACAACATGGTG+TGG + chr7.4:34061283-34061302 MS.gene95656:intron 35.0%
CTTGTTAATTAGTGCTCTCA+GGG - chr7.4:34062102-34062121 None:intergenic 35.0%
CTTTCTTGCTTCTATACATC+TGG - chr7.4:34061679-34061698 None:intergenic 35.0%
TCATGTGAAGAGTCATTTAC+AGG + chr7.4:34061539-34061558 MS.gene95656:CDS 35.0%
TCTTGCCATGTATGAAAACA+TGG + chr7.4:34062442-34062461 MS.gene95656:CDS 35.0%
TCTTGTTAATTAGTGCTCTC+AGG - chr7.4:34062103-34062122 None:intergenic 35.0%
TTACAGATGTGGATCATATG+GGG + chr7.4:34062412-34062431 MS.gene95656:intron 35.0%
TTGTTAATTAGTGCTCTCAG+GGG - chr7.4:34062101-34062120 None:intergenic 35.0%
TTTGGAAAATACTATGACCC+TGG - chr7.4:34062550-34062569 None:intergenic 35.0%
! AATTTTACAACTGTCGTTGG+AGG - chr7.4:34062354-34062373 None:intergenic 35.0%
! AGAAGAACAAGATGGTTCTA+AGG + chr7.4:34062907-34062926 MS.gene95656:CDS 35.0%
! AGTTCTTCCTCTTGATTTTC+AGG - chr7.4:34062719-34062738 None:intergenic 35.0%
! GGGTCATAGTATTTTCCAAA+TGG + chr7.4:34062550-34062569 MS.gene95656:CDS 35.0%
! TAACTTGCCTGAAAATCAAG+AGG + chr7.4:34062709-34062728 MS.gene95656:CDS 35.0%
ATGTTAGATCCAAGTTGCCT+AGG + chr7.4:34061124-34061143 MS.gene95656:CDS 40.0%
ATTCATGGCCACAAAATGAG+AGG - chr7.4:34062634-34062653 None:intergenic 40.0%
CATAGGCTTCTTGTAGACAA+TGG + chr7.4:34061844-34061863 MS.gene95656:CDS 40.0%
GAAATGAGATCTTGTGCTCT+TGG - chr7.4:34062683-34062702 None:intergenic 40.0%
GATGTGCAAAAGAACAGCAA+TGG + chr7.4:34061036-34061055 MS.gene95656:CDS 40.0%
GGCAACTTGGATCTAACATA+AGG - chr7.4:34061123-34061142 None:intergenic 40.0%
TAACAGTCTTGGCTGATCAT+AGG + chr7.4:34061827-34061846 MS.gene95656:intron 40.0%
TGTAGGAGCAACTCCAAAAT+TGG + chr7.4:34061479-34061498 MS.gene95656:intron 40.0%
! GAATCTAGAGCTGAGTTCTA+TGG + chr7.4:34062485-34062504 MS.gene95656:CDS 40.0%
CTAACATAAGGCCTCACTGA+TGG - chr7.4:34061111-34061130 None:intergenic 45.0%
CTAACTAGTACCCATGCCAA+GGG + chr7.4:34061992-34062011 MS.gene95656:intron 45.0%
CTTCATGCTGTGCAAAGACT+TGG + chr7.4:34061180-34061199 MS.gene95656:CDS 45.0%
GTGAGAAGAAACCATCAGTG+AGG + chr7.4:34061097-34061116 MS.gene95656:CDS 45.0%
GTGCAAAAGAACAGCAATGG+AGG + chr7.4:34061039-34061058 MS.gene95656:CDS 45.0%
! ATGCTGTGCAAAGACTTGGT+GGG + chr7.4:34061184-34061203 MS.gene95656:CDS 45.0%
! GGAGTATAGCCTTGAAGCAT+GGG - chr7.4:34061901-34061920 None:intergenic 45.0%
! TGGAGTATAGCCTTGAAGCA+TGG - chr7.4:34061902-34061921 None:intergenic 45.0%
!! GGTTCTAAGGAGCAAGAGAA+AGG + chr7.4:34062920-34062939 MS.gene95656:CDS 45.0%
!!! TAACTTTTTTTTTTTTTTTA+TGG + chr7.4:34061963-34061982 MS.gene95656:intron 5.0%
ACCCAACTTCCCATGCTTCA+AGG + chr7.4:34061889-34061908 MS.gene95656:CDS 50.0%
AGCCTTGAAGCATGGGAAGT+TGG - chr7.4:34061894-34061913 None:intergenic 50.0%
CATGCTGTGCAAAGACTTGG+TGG + chr7.4:34061183-34061202 MS.gene95656:CDS 50.0%
GCCTTGAAGCATGGGAAGTT+GGG - chr7.4:34061893-34061912 None:intergenic 50.0%
GCTAACTAGTACCCATGCCA+AGG + chr7.4:34061991-34062010 MS.gene95656:intron 50.0%
GCTTAACCAATGCATGCCCT+TGG - chr7.4:34062011-34062030 None:intergenic 50.0%
ACCAATGCATGCCCTTGGCA+TGG - chr7.4:34062006-34062025 None:intergenic 55.0%
CCAACGTAGCCTAGGCAACT+TGG - chr7.4:34061136-34061155 None:intergenic 55.0%
CCAAGTTGCCTAGGCTACGT+TGG + chr7.4:34061133-34061152 MS.gene95656:CDS 55.0%
CCAATGCATGCCCTTGGCAT+GGG - chr7.4:34062005-34062024 None:intergenic 55.0%
TCAGGAGTCCAACGTAGCCT+AGG - chr7.4:34061144-34061163 None:intergenic 55.0%
! CCCATGCCAAGGGCATGCAT+TGG + chr7.4:34062002-34062021 MS.gene95656:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 34060940 34062973 34060940 ID=MS.gene95656
chr7.4 mRNA 34060940 34062973 34060940 ID=MS.gene95656.t1;Parent=MS.gene95656
chr7.4 exon 34060940 34061213 34060940 ID=MS.gene95656.t1.exon1;Parent=MS.gene95656.t1
chr7.4 CDS 34060940 34061213 34060940 ID=cds.MS.gene95656.t1;Parent=MS.gene95656.t1
chr7.4 exon 34061484 34061560 34061484 ID=MS.gene95656.t1.exon2;Parent=MS.gene95656.t1
chr7.4 CDS 34061484 34061560 34061484 ID=cds.MS.gene95656.t1;Parent=MS.gene95656.t1
chr7.4 exon 34061680 34061716 34061680 ID=MS.gene95656.t1.exon3;Parent=MS.gene95656.t1
chr7.4 CDS 34061680 34061716 34061680 ID=cds.MS.gene95656.t1;Parent=MS.gene95656.t1
chr7.4 exon 34061833 34061941 34061833 ID=MS.gene95656.t1.exon4;Parent=MS.gene95656.t1
chr7.4 CDS 34061833 34061941 34061833 ID=cds.MS.gene95656.t1;Parent=MS.gene95656.t1
chr7.4 exon 34062418 34062973 34062418 ID=MS.gene95656.t1.exon5;Parent=MS.gene95656.t1
chr7.4 CDS 34062418 34062973 34062418 ID=cds.MS.gene95656.t1;Parent=MS.gene95656.t1
Gene Sequence

>MS.gene95656

ATGAATATGAGAGAGGAAGAAACATATGGATCAGAATGTTCCAAGGCAAGTGTTTCAATTAATGAAGATGATTGTGAAATTAGTGAAGGAAATGAAGATGTGCAAAAGAACAGCAATGGAGGAATAAGCTCAAGCAATAGTACTATAGAAGAGAATTGTGAGAAGAAACCATCAGTGAGGCCTTATGTTAGATCCAAGTTGCCTAGGCTACGTTGGACTCCTGATCTTCATTTTCGTTTTCTTCATGCTGTGCAAAGACTTGGTGGGCAAGAGAGTAAGTTCCACACACATAACATATATGTATATTTTTCTTATAATTATTAGCTAAGAATTGAAAAGTAACCTAGAATTTACAACATGGTGTGGACCATGATGATCAAACACATAGATATAGCTTCTTTTTGCCTTTTCTAGATTTCAATTTTTCATAGGGGAATTCAATATCAAGTTAGTCCTAGGTAGGTTAATCAATTCAACAATATGATTATTCTTGTTATTTTAATTTTAATTTTGTATGTATTGTTAATGATTTTCTTGTGTGTAGGAGCAACTCCAAAATTGGTTCTTCAATTGATGAATATAAAAGGTTTAAGTATTGCTCATGTGAAGAGTCATTTACAGGTAAAAAGTCATAGATAAATATGGTTGCAACCTATATATAGCTAGCTAGCTTTCAATATTTCTTAAGATTTATAATTGTTTAATTAATTTCTTTTCTTTCACATGTATTTCTTTTCCAGATGTATAGAAGCAAGAAAGTAGTTGACACAAATCAAGGTACAATAAGGTTTTTCTTTTCTTTTTTGTATGAATTCAACTATCAATAAGTGATTACATAATTAGGGTTTGTGCTTGCAAGTCACTAATTATTTATGATTGTATATATGTAACAGTCTTGGCTGATCATAGGCTTCTTGTAGACAATGGAGACAGAAATGTTTATAATCTCACCCAACTTCCCATGCTTCAAGGCTATACTCCAAATCAAACATCATCATATAGGTATACATATACTACTTGAATTAACTTTTTTTTTTTTTTTATGGAGAATGCTAACTAGTACCCATGCCAAGGGCATGCATTGGTTAAGCATCTAATCAAAAGTAACGTTTTATATTTCAAAGACAAAAATTCTATTTGTAGAATCTTTAATCATTACCCCTGAGAGCACTAATTAACAAGACCTTTTTTTTATTCATAGGGATCATATAATCCCTTCTCGTTTTTCGATAAGAATAAAGTTTCAAAACAAGTACAAAGGGTACTAAAATCGAGTTACTGAACTTGACCAAAAAATAAAATTAAAAAAATCGAGTTACACAAGAACAAAGTACGAGTTGCTAACCTTTGTTCCAAATTTCCTTAAGTCATTTACGTTTAAAATTGTAACCTCAAGGTTTAAACTTCAAATCCTCCAACGACAGTTGTAAAATTACTATTATTGTCTTAGAATTGAAGGTTTTATTTTTTTTTTACAGATGTGGATCATATGGGGATGCTTCTCTTGCCATGTATGAAAACATGGTACAAATGAGTTCAATAAGTGAATCTAGAGCTGAGTTCTATGGCAAAATGATTGAGAGAACCAATAATAGCAACATCAATATCCAGGGTCATAGTATTTTCCAAATGGATTCATCCAATTTTAGAGAACTATCAACAACTAAAGTCCATGAACCAAATGATAATTTCCTCTCATTTTGTGGCCATGAATCTTTAAGAGATGATCTACAAGTTCAGCCAAGAGCACAAGATCTCATTTCGAACGATAACTTGCCTGAAAATCAAGAGGAAGAACTGAAAACAACGAAAAGAAAATCTTCAAATATGGATCTTGAGTTGGATCTATCACTAAAATTAAATTCAAGGAGCAATGGTGATCATAAATGTATAGAGGAGCATGAAATTGATAGCAATTTATCACTCTCTTTGTATTCTCAATCATCTTATGTTTGCAAAAGGATGAAAGAAGAACAAGATGGTTCTAAGGAGCAAGAGAAAGGAGTCAATATTTCTTTGGATCTAACCATATGA
Protein sequence

>MS.gene95656.t1

MNMREEETYGSECSKASVSINEDDCEISEGNEDVQKNSNGGISSSNSTIEENCEKKPSVRPYVRSKLPRLRWTPDLHFRFLHAVQRLGGQERATPKLVLQLMNIKGLSIAHVKSHLQMYRSKKVVDTNQVLADHRLLVDNGDRNVYNLTQLPMLQGYTPNQTSSYRCGSYGDASLAMYENMVQMSSISESRAEFYGKMIERTNNSNINIQGHSIFQMDSSNFRELSTTKVHEPNDNFLSFCGHESLRDDLQVQPRAQDLISNDNLPENQEEELKTTKRKSSNMDLELDLSLKLNSRSNGDHKCIEEHEIDSNLSLSLYSQSSYVCKRMKEEQDGSKEQEKGVNISLDLTI