AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95670.t1 MTR_1g013130 39.200 125 61 5 63 179 76 193 1.72e-12 66.2
MS.gene95670.t1 MTR_3g011990 43.678 87 32 3 67 146 72 148 1.95e-11 63.5
MS.gene95670.t1 MTR_3g012010 49.254 67 27 1 66 125 71 137 2.19e-11 63.5
MS.gene95670.t1 MTR_8g466760 46.667 75 27 3 77 148 86 150 4.00e-11 62.8
MS.gene95670.t1 MTR_7g026977 36.111 108 60 2 26 126 83 188 6.19e-11 62.4
MS.gene95670.t1 MTR_3g065020 71.795 39 9 1 80 118 82 118 9.51e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95670.t1 AT5G10970 39.837 123 48 6 63 179 84 186 1.31e-11 63.9
MS.gene95670.t1 AT5G25160 41.837 98 48 4 82 179 57 145 1.72e-11 63.2
MS.gene95670.t1 AT1G80730 68.293 41 11 1 78 118 60 98 5.12e-11 61.6

Find 81 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCAATGCCTTTCCTTCT+TGG 0.138422 7.4:+33826453 MS.gene95670:CDS
TTTGATTTCGACTCTAATTT+TGG 0.159297 7.4:-33826041 None:intergenic
TGAATTATGCCTTCTCTATT+AGG 0.184623 7.4:-33826728 None:intergenic
CCCCAAGCAACAACACAATT+TGG 0.187401 7.4:+33826563 MS.gene95670:CDS
TCTTGGCTCGGTTCACCTTA+TGG 0.231125 7.4:+33826470 MS.gene95670:CDS
TGGTGTGAGTGCCAAGTCTT+AGG 0.231168 7.4:-33826121 None:intergenic
CGGGTTGCAAAGGGTGTATT+TGG 0.256836 7.4:-33826515 None:intergenic
CCCAAATTGTGTTGTTGCTT+GGG 0.273537 7.4:-33826564 None:intergenic
GTTGTTGCTTGGGGTGAATT+TGG 0.275394 7.4:-33826554 None:intergenic
CCCAAAGTGTTTGACCTTTG+TGG 0.286115 7.4:-33826632 None:intergenic
GTTCACGCTTATGAGCATTT+TGG 0.317761 7.4:-33826292 None:intergenic
AGGTCAAACACTTTGGGTTT+GGG 0.338136 7.4:+33826638 MS.gene95670:CDS
CCACAAAGGTCAAACACTTT+GGG 0.340923 7.4:+33826632 MS.gene95670:CDS
GTGTTTGACCTTTGTGGAAT+TGG 0.343209 7.4:-33826626 None:intergenic
AAGGTCAAACACTTTGGGTT+TGG 0.344659 7.4:+33826637 MS.gene95670:CDS
CAAGGGTTTGGAAGTGGACT+TGG 0.360530 7.4:-33826141 None:intergenic
GTCACATATTGGACGCCAAT+TGG 0.375665 7.4:-33826434 None:intergenic
TCCCAAATTGTGTTGTTGCT+TGG 0.377648 7.4:-33826565 None:intergenic
TTCTCCACTTCTCAAGCTCT+TGG 0.384550 7.4:+33826263 MS.gene95670:CDS
TAGAAACCTCAAACGCAAAA+TGG 0.385914 7.4:+33826010 MS.gene95670:CDS
TCCACCGTTCTCCAAGGGTT+TGG 0.389944 7.4:-33826153 None:intergenic
CACCTTATGGTGGCTATGGA+GGG 0.398816 7.4:+33826483 MS.gene95670:CDS
GAAAGGCATTGGTCACATAT+TGG 0.406502 7.4:-33826445 None:intergenic
TAATAGAGAAGGCATAATTC+AGG 0.407241 7.4:+33826730 MS.gene95670:CDS
CCCAAGCAACAACACAATTT+GGG 0.410223 7.4:+33826564 MS.gene95670:CDS
TCCACAAAGGTCAAACACTT+TGG 0.411221 7.4:+33826631 MS.gene95670:CDS
TTAGGATCACGAGATAATAA+TGG 0.420098 7.4:-33826710 None:intergenic
ACCACTTTCTGCTCAGGTAA+AGG 0.442903 7.4:-33826209 None:intergenic
TCAAGCTCTATTCACTATCA+AGG 0.445678 7.4:+33826377 MS.gene95670:CDS
AATGCCTTTCCTTCTTGGCT+CGG 0.453136 7.4:+33826458 MS.gene95670:CDS
TGATCAATGAAATTTCTTTG+AGG 0.466939 7.4:-33826668 None:intergenic
TTGGCATTTGCATCACATAC+CGG 0.471216 7.4:-33826535 None:intergenic
GTTCTCCAAGGGTTTGGAAG+TGG 0.473154 7.4:-33826147 None:intergenic
TCACATATTGGACGCCAATT+GGG 0.475144 7.4:-33826433 None:intergenic
TCACCTTATGGTGGCTATGG+AGG 0.475493 7.4:+33826482 MS.gene95670:CDS
TCCACTTCTCAAGCTCTTGG+AGG 0.477317 7.4:+33826266 MS.gene95670:CDS
GCTAACTTGCACAACCCAAT+TGG 0.486494 7.4:+33826419 MS.gene95670:CDS
GGTGTGAGTGCCAAGTCTTA+GGG 0.491546 7.4:-33826120 None:intergenic
TAAAAGGAGAGAGGAAGAGA+TGG 0.501837 7.4:+33826337 MS.gene95670:CDS
GGTTCACCTTATGGTGGCTA+TGG 0.502207 7.4:+33826479 MS.gene95670:CDS
AACTCTACCACTTTCTGCTC+AGG 0.502340 7.4:-33826215 None:intergenic
CCAAATTGTGTTGTTGCTTG+GGG 0.502858 7.4:-33826563 None:intergenic
CAAGTCCACTTCCAAACCCT+TGG 0.519689 7.4:+33826142 MS.gene95670:CDS
ATCACATACCGGGTTGCAAA+GGG 0.522044 7.4:-33826524 None:intergenic
ACCCCTCCATAGCCACCATA+AGG 0.522093 7.4:-33826485 None:intergenic
GTTGATGATGAGGACACATT+GGG 0.531489 7.4:-33826758 None:intergenic
TGGCATTTGCATCACATACC+GGG 0.531856 7.4:-33826534 None:intergenic
TCTATCCTCATCATTTCCAA+TGG 0.532227 7.4:-33825991 None:intergenic
ATTTCCAATGGAAATTCTCT+CGG 0.535410 7.4:-33825979 None:intergenic
ACCTTATGGTGGCTATGGAG+GGG 0.537456 7.4:+33826484 MS.gene95670:CDS
ATTAGAGTCGAAATCAAAGA+AGG 0.542585 7.4:+33826046 MS.gene95670:CDS
CAAATACACCCTTTGCAACC+CGG 0.543155 7.4:+33826516 MS.gene95670:CDS
GAATAGAGCTTGAAAACCAT+AGG 0.547197 7.4:-33826367 None:intergenic
TCAACATCAGATTCAGAGGT+TGG 0.549087 7.4:-33826086 None:intergenic
TAGCACTACGGAAATATGAA+TGG 0.550571 7.4:-33826400 None:intergenic
AACCTCTGAATCTGATGTTG+AGG 0.555993 7.4:+33826088 MS.gene95670:CDS
ACCTCCAAGAGCTTGAGAAG+TGG 0.556669 7.4:-33826267 None:intergenic
AATGGAAGATAAAAGGAGAG+AGG 0.557828 7.4:+33826328 MS.gene95670:CDS
TGAATGTCCACCGTTCTCCA+AGG 0.561681 7.4:-33826159 None:intergenic
CATCACATACCGGGTTGCAA+AGG 0.564146 7.4:-33826525 None:intergenic
AAGAGATGGATTCAACCCTA+TGG 0.564603 7.4:+33826351 MS.gene95670:CDS
GAATGTCCACCGTTCTCCAA+GGG 0.565536 7.4:-33826158 None:intergenic
ACCTTTACCTGAGCAGAAAG+TGG 0.574184 7.4:+33826208 MS.gene95670:CDS
TAAGGTGAACCGAGCCAAGA+AGG 0.575815 7.4:-33826467 None:intergenic
TGAACCGAGCCAAGAAGGAA+AGG 0.582959 7.4:-33826462 None:intergenic
GAGCCAAGAAGGAAAGGCAT+TGG 0.584408 7.4:-33826456 None:intergenic
ATGTCCGAGAGAATTTCCAT+TGG 0.586763 7.4:+33825975 MS.gene95670:CDS
AATAGAGCTTGAAAACCATA+GGG 0.593787 7.4:-33826366 None:intergenic
ATCCTCAACATCAGATTCAG+AGG 0.595365 7.4:-33826090 None:intergenic
AGTTGATGATGAGGACACAT+TGG 0.595890 7.4:-33826759 None:intergenic
TCTCGTGATCCTAATAGAGA+AGG 0.597683 7.4:+33826719 MS.gene95670:CDS
AATTTCCATTGGAAATGATG+AGG 0.604935 7.4:+33825986 MS.gene95670:CDS
CCTCAAACGCAAAATGGTGG+AGG 0.605406 7.4:+33826016 MS.gene95670:CDS
GATTTACTAACCCTAAGACT+TGG 0.606458 7.4:+33826110 MS.gene95670:CDS
ACTTCCAAACCCTTGGAGAA+CGG 0.610472 7.4:+33826149 MS.gene95670:CDS
TGGCTCGGTTCACCTTATGG+TGG 0.610898 7.4:+33826473 MS.gene95670:CDS
AAACCTCAAACGCAAAATGG+TGG 0.616518 7.4:+33826013 MS.gene95670:CDS
GGTTGTGCAAGTTAGCACTA+CGG 0.648362 7.4:-33826412 None:intergenic
TCCAAACCCTTGGAGAACGG+TGG 0.656704 7.4:+33826152 MS.gene95670:CDS
CTGCATTTCCAATTCCACAA+AGG 0.667747 7.4:+33826618 MS.gene95670:CDS
CGTTGATTGAGTTGATGATG+AGG 0.670107 7.4:-33826768 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAATGGAAGATAAA+AGG + chr7.4:33826321-33826340 MS.gene95670:CDS 15.0%
! TGAACGTGTCTTAAAAAAAA+TGG + chr7.4:33826310-33826329 MS.gene95670:CDS 25.0%
! TGATCAATGAAATTTCTTTG+AGG - chr7.4:33826671-33826690 None:intergenic 25.0%
!!! AATTTGGGATGACTAATTTT+TGG + chr7.4:33826579-33826598 MS.gene95670:CDS 25.0%
!!! ATTTGGGATGACTAATTTTT+GGG + chr7.4:33826580-33826599 MS.gene95670:CDS 25.0%
!!! TTTGATTTCGACTCTAATTT+TGG - chr7.4:33826044-33826063 None:intergenic 25.0%
AATAGAGCTTGAAAACCATA+GGG - chr7.4:33826369-33826388 None:intergenic 30.0%
AATTTCCATTGGAAATGATG+AGG + chr7.4:33825986-33826005 MS.gene95670:CDS 30.0%
ATTAGAGTCGAAATCAAAGA+AGG + chr7.4:33826046-33826065 MS.gene95670:CDS 30.0%
ATTTCCAATGGAAATTCTCT+CGG - chr7.4:33825982-33826001 None:intergenic 30.0%
TGAATTATGCCTTCTCTATT+AGG - chr7.4:33826731-33826750 None:intergenic 30.0%
TTAGGATCACGAGATAATAA+TGG - chr7.4:33826713-33826732 None:intergenic 30.0%
! TAATAGAGAAGGCATAATTC+AGG + chr7.4:33826730-33826749 MS.gene95670:CDS 30.0%
!! TTTGGGATGACTAATTTTTG+GGG + chr7.4:33826581-33826600 MS.gene95670:CDS 30.0%
AATGGAAGATAAAAGGAGAG+AGG + chr7.4:33826328-33826347 MS.gene95670:CDS 35.0%
GAATAGAGCTTGAAAACCAT+AGG - chr7.4:33826370-33826389 None:intergenic 35.0%
GATTTACTAACCCTAAGACT+TGG + chr7.4:33826110-33826129 MS.gene95670:CDS 35.0%
TAGAAACCTCAAACGCAAAA+TGG + chr7.4:33826010-33826029 MS.gene95670:CDS 35.0%
TAGCACTACGGAAATATGAA+TGG - chr7.4:33826403-33826422 None:intergenic 35.0%
TCAAGCTCTATTCACTATCA+AGG + chr7.4:33826377-33826396 MS.gene95670:CDS 35.0%
TCTATCCTCATCATTTCCAA+TGG - chr7.4:33825994-33826013 None:intergenic 35.0%
AAACCTCAAACGCAAAATGG+TGG + chr7.4:33826013-33826032 MS.gene95670:CDS 40.0%
AACCTCTGAATCTGATGTTG+AGG + chr7.4:33826088-33826107 MS.gene95670:CDS 40.0%
ATCCTCAACATCAGATTCAG+AGG - chr7.4:33826093-33826112 None:intergenic 40.0%
ATGTCCGAGAGAATTTCCAT+TGG + chr7.4:33825975-33825994 MS.gene95670:CDS 40.0%
CCACAAAGGTCAAACACTTT+GGG + chr7.4:33826632-33826651 MS.gene95670:CDS 40.0%
CCCAAGCAACAACACAATTT+GGG + chr7.4:33826564-33826583 MS.gene95670:CDS 40.0%
CTGCATTTCCAATTCCACAA+AGG + chr7.4:33826618-33826637 MS.gene95670:CDS 40.0%
GTGTTTGACCTTTGTGGAAT+TGG - chr7.4:33826629-33826648 None:intergenic 40.0%
TAAAAGGAGAGAGGAAGAGA+TGG + chr7.4:33826337-33826356 MS.gene95670:CDS 40.0%
TCAACATCAGATTCAGAGGT+TGG - chr7.4:33826089-33826108 None:intergenic 40.0%
TCACATATTGGACGCCAATT+GGG - chr7.4:33826436-33826455 None:intergenic 40.0%
TCCACAAAGGTCAAACACTT+TGG + chr7.4:33826631-33826650 MS.gene95670:CDS 40.0%
TCTCGTGATCCTAATAGAGA+AGG + chr7.4:33826719-33826738 MS.gene95670:CDS 40.0%
TTGGCATTTGCATCACATAC+CGG - chr7.4:33826538-33826557 None:intergenic 40.0%
! AAGAGATGGATTCAACCCTA+TGG + chr7.4:33826351-33826370 MS.gene95670:CDS 40.0%
! AAGGTCAAACACTTTGGGTT+TGG + chr7.4:33826637-33826656 MS.gene95670:CDS 40.0%
! AGGTCAAACACTTTGGGTTT+GGG + chr7.4:33826638-33826657 MS.gene95670:CDS 40.0%
! CCAAATTGTGTTGTTGCTTG+GGG - chr7.4:33826566-33826585 None:intergenic 40.0%
! CCCAAATTGTGTTGTTGCTT+GGG - chr7.4:33826567-33826586 None:intergenic 40.0%
! GTTCACGCTTATGAGCATTT+TGG - chr7.4:33826295-33826314 None:intergenic 40.0%
! TCCCAAATTGTGTTGTTGCT+TGG - chr7.4:33826568-33826587 None:intergenic 40.0%
!! AGTTGATGATGAGGACACAT+TGG - chr7.4:33826762-33826781 None:intergenic 40.0%
!! CGTTGATTGAGTTGATGATG+AGG - chr7.4:33826771-33826790 None:intergenic 40.0%
!! GAAAGGCATTGGTCACATAT+TGG - chr7.4:33826448-33826467 None:intergenic 40.0%
!! GTTGATGATGAGGACACATT+GGG - chr7.4:33826761-33826780 None:intergenic 40.0%
!!! ATGACTAATTTTTGGGGTGG+AGG + chr7.4:33826587-33826606 MS.gene95670:CDS 40.0%
AACTCTACCACTTTCTGCTC+AGG - chr7.4:33826218-33826237 None:intergenic 45.0%
AATGCCTTTCCTTCTTGGCT+CGG + chr7.4:33826458-33826477 MS.gene95670:CDS 45.0%
ACCACTTTCTGCTCAGGTAA+AGG - chr7.4:33826212-33826231 None:intergenic 45.0%
ACCTTTACCTGAGCAGAAAG+TGG + chr7.4:33826208-33826227 MS.gene95670:CDS 45.0%
ACTTCCAAACCCTTGGAGAA+CGG + chr7.4:33826149-33826168 MS.gene95670:CDS 45.0%
ATCACATACCGGGTTGCAAA+GGG - chr7.4:33826527-33826546 None:intergenic 45.0%
CAAATACACCCTTTGCAACC+CGG + chr7.4:33826516-33826535 MS.gene95670:CDS 45.0%
CCCCAAGCAACAACACAATT+TGG + chr7.4:33826563-33826582 MS.gene95670:CDS 45.0%
GGTTGTGCAAGTTAGCACTA+CGG - chr7.4:33826415-33826434 None:intergenic 45.0%
GTCACATATTGGACGCCAAT+TGG - chr7.4:33826437-33826456 None:intergenic 45.0%
TGACCAATGCCTTTCCTTCT+TGG + chr7.4:33826453-33826472 MS.gene95670:CDS 45.0%
TGGCATTTGCATCACATACC+GGG - chr7.4:33826537-33826556 None:intergenic 45.0%
TTCTCCACTTCTCAAGCTCT+TGG + chr7.4:33826263-33826282 MS.gene95670:CDS 45.0%
! CCCAAAGTGTTTGACCTTTG+TGG - chr7.4:33826635-33826654 None:intergenic 45.0%
! GCTAACTTGCACAACCCAAT+TGG + chr7.4:33826419-33826438 MS.gene95670:CDS 45.0%
! GTTGTTGCTTGGGGTGAATT+TGG - chr7.4:33826557-33826576 None:intergenic 45.0%
!! GGGATGACTAATTTTTGGGG+TGG + chr7.4:33826584-33826603 MS.gene95670:CDS 45.0%
ACCTCCAAGAGCTTGAGAAG+TGG - chr7.4:33826270-33826289 None:intergenic 50.0%
ACCTTATGGTGGCTATGGAG+GGG + chr7.4:33826484-33826503 MS.gene95670:CDS 50.0%
CAAGTCCACTTCCAAACCCT+TGG + chr7.4:33826142-33826161 MS.gene95670:CDS 50.0%
CACCTTATGGTGGCTATGGA+GGG + chr7.4:33826483-33826502 MS.gene95670:CDS 50.0%
CATCACATACCGGGTTGCAA+AGG - chr7.4:33826528-33826547 None:intergenic 50.0%
CCTCAAACGCAAAATGGTGG+AGG + chr7.4:33826016-33826035 MS.gene95670:CDS 50.0%
GAATGTCCACCGTTCTCCAA+GGG - chr7.4:33826161-33826180 None:intergenic 50.0%
GGTGTGAGTGCCAAGTCTTA+GGG - chr7.4:33826123-33826142 None:intergenic 50.0%
GGTTCACCTTATGGTGGCTA+TGG + chr7.4:33826479-33826498 MS.gene95670:CDS 50.0%
GTTCTCCAAGGGTTTGGAAG+TGG - chr7.4:33826150-33826169 None:intergenic 50.0%
TAAGGTGAACCGAGCCAAGA+AGG - chr7.4:33826470-33826489 None:intergenic 50.0%
TCACCTTATGGTGGCTATGG+AGG + chr7.4:33826482-33826501 MS.gene95670:CDS 50.0%
TCCACTTCTCAAGCTCTTGG+AGG + chr7.4:33826266-33826285 MS.gene95670:CDS 50.0%
TGAACCGAGCCAAGAAGGAA+AGG - chr7.4:33826465-33826484 None:intergenic 50.0%
TGAATGTCCACCGTTCTCCA+AGG - chr7.4:33826162-33826181 None:intergenic 50.0%
TGGTGTGAGTGCCAAGTCTT+AGG - chr7.4:33826124-33826143 None:intergenic 50.0%
! CAAGGGTTTGGAAGTGGACT+TGG - chr7.4:33826144-33826163 None:intergenic 50.0%
! CCTCCACCATTTTGCGTTTG+AGG - chr7.4:33826019-33826038 None:intergenic 50.0%
! CGGGTTGCAAAGGGTGTATT+TGG - chr7.4:33826518-33826537 None:intergenic 50.0%
! GAGCCAAGAAGGAAAGGCAT+TGG - chr7.4:33826459-33826478 None:intergenic 50.0%
!! TCTTGGCTCGGTTCACCTTA+TGG + chr7.4:33826470-33826489 MS.gene95670:CDS 50.0%
ACCCCTCCATAGCCACCATA+AGG - chr7.4:33826488-33826507 None:intergenic 55.0%
TCCAAACCCTTGGAGAACGG+TGG + chr7.4:33826152-33826171 MS.gene95670:CDS 55.0%
TCCACCGTTCTCCAAGGGTT+TGG - chr7.4:33826156-33826175 None:intergenic 55.0%
!! TGGCTCGGTTCACCTTATGG+TGG + chr7.4:33826473-33826492 MS.gene95670:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 33825975 33826793 33825975 ID=MS.gene95670
chr7.4 mRNA 33825975 33826793 33825975 ID=MS.gene95670.t1;Parent=MS.gene95670
chr7.4 exon 33825975 33826793 33825975 ID=MS.gene95670.t1.exon1;Parent=MS.gene95670.t1
chr7.4 CDS 33825975 33826793 33825975 ID=cds.MS.gene95670.t1;Parent=MS.gene95670.t1
Gene Sequence

>MS.gene95670

ATGTCCGAGAGAATTTCCATTGGAAATGATGAGGATAGAAACCTCAAACGCAAAATGGTGGAGGAGCCAAAATTAGAGTCGAAATCAAAGAAGGAGTCAAAGACAATGACACCAACCTCTGAATCTGATGTTGAGGATTTACTAACCCTAAGACTTGGCACTCACACCAAGTCCACTTCCAAACCCTTGGAGAACGGTGGACATTCAAGTTCATTTCAAAACACTACTGATGAACCTTTACCTGAGCAGAAAGTGGTAGAGTTCTCTTGTAATTATTGTGACAAGAAATTCTCCACTTCTCAAGCTCTTGGAGGTCACCAAAATGCTCATAAGCGTGAACGTGTCTTAAAAAAAATGGAAGATAAAAGGAGAGAGGAAGAGATGGATTCAACCCTATGGTTTTCAAGCTCTATTCACTATCAAGGCCATTCATATTTCCGTAGTGCTAACTTGCACAACCCAATTGGCGTCCAATATGTGACCAATGCCTTTCCTTCTTGGCTCGGTTCACCTTATGGTGGCTATGGAGGGGTGTATATGCCAAATACACCCTTTGCAACCCGGTATGTGATGCAAATGCCAAATTCACCCCAAGCAACAACACAATTTGGGATGACTAATTTTTGGGGTGGAGGTCAAAATGCTGCATTTCCAATTCCACAAAGGTCAAACACTTTGGGTTTGGGACTTTTTCCTCAAAGAAATTTCATTGATCAACTTCGTTCTCATACTCTTCCATTATTATCTCGTGATCCTAATAGAGAAGGCATAATTCAGGCAAATCCCAATGTGTCCTCATCATCAACTCAATCAACGTAG

Protein sequence

>MS.gene95670.t1

MSERISIGNDEDRNLKRKMVEEPKLESKSKKESKTMTPTSESDVEDLLTLRLGTHTKSTSKPLENGGHSSSFQNTTDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDKRREEEMDSTLWFSSSIHYQGHSYFRSANLHNPIGVQYVTNAFPSWLGSPYGGYGGVYMPNTPFATRYVMQMPNSPQATTQFGMTNFWGGGQNAAFPIPQRSNTLGLGLFPQRNFIDQLRSHTLPLLSRDPNREGIIQANPNVSSSSTQST