AlfalfaGEDB | MS.gene95951

M. sativa cultivar XinJiangDaYe / MS.gene95951

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95951.t1	XP_013455733.1	59.8	239	39	4	1	187	1	234	6.90E-60	240.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95951.t1	Q42498	27.3	165	116	3	9	170	2	165	2.0e-07	57.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95951.t1	A0A072UJ33	59.8	239	39	4	1	187	1	234	5.0e-60	240.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene95951.t1	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95951.t1	MTR_4g051538	61.506	239	35	4	1	187	1	234	1.20e-82	244
MS.gene95951.t1	MTR_3g104630	37.566	189	55	5	1	173	1	142	3.55e-27	100
MS.gene95951.t1	MTR_3g052920	52.830	53	25	0	5	57	5	57	1.15e-12	64.3
MS.gene95951.t1	MTR_8g043650	49.123	57	29	0	1	57	1	57	2.04e-12	63.9
MS.gene95951.t1	MTR_1g012570	50.943	53	26	0	5	57	5	57	2.19e-12	63.9
MS.gene95951.t1	MTR_3g052870	50.943	53	26	0	5	57	5	57	2.50e-12	63.2
MS.gene95951.t1	MTR_1g105920	50.943	53	26	0	5	57	5	57	3.55e-12	63.2
MS.gene95951.t1	MTR_1g105910	50.943	53	26	0	5	57	5	57	3.89e-12	63.2
MS.gene95951.t1	MTR_1g106070	49.123	57	29	0	1	57	1	57	4.81e-12	62.0
MS.gene95951.t1	MTR_2g030740	50.943	53	26	0	5	57	5	57	6.69e-12	62.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95951.t1	AT4G36590	49.057	53	27	0	5	57	3	55	3.49e-12	63.5

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Find 28 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCTGGAATTTACAACA+AGG	0.757794	6.2:+76529574	MS.gene95951:CDS
AACAGAAAAGATCATGAAAT+TGG	0.332742	6.2:+76530170	MS.gene95951:CDS
AACCTTATTGGTGCAAGCGG+AGG	0.596051	6.2:+76530526	MS.gene95951:CDS
AAGATGTACACATACCACGA+GGG	0.757349	6.2:-76529980	None:intergenic
AAGCATGAGAATATCAACTG+AGG	0.718686	6.2:-76529624	None:intergenic
ACACAAGGAGTCAACCTTAT+TGG	0.323352	6.2:+76530514	MS.gene95951:CDS
ATCCTCCGCTTGCACCAATA+AGG	0.357163	6.2:-76530528	None:intergenic
CCGCCGAAGAGGTCATCAAG+AGG	0.664394	6.2:+76529485	MS.gene95951:CDS
CCTCTTGATGACCTCTTCGG+CGG	0.600952	6.2:-76529485	None:intergenic
CTGAGTTAACAACACTTTCA+GGG	0.482866	6.2:+76529600	MS.gene95951:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCAAGAGGAAAACAAAAAG+TGG	+	chr6.2:76529499-76529518	MS.gene95951:CDS	30.0%
ATCATTTCCTCTTCTATTGA+AGG	-	chr6.2:76529726-76529745	None:intergenic	30.0%
GAAACTATAATGGTAAAAGC+TGG	-	chr6.2:76530311-76530330	None:intergenic	30.0%
TATGTATTGTCTTCCTTTCA+TGG	+	chr6.2:76530430-76530449	MS.gene95951:intron	30.0%
TCCCATAAGTGAAACTATAA+TGG	-	chr6.2:76530321-76530340	None:intergenic	30.0%
TGAAAATCCTTCAATAGAAG+AGG	+	chr6.2:76529716-76529735	MS.gene95951:intron	30.0%
TGTAGTAATTTCTACTATCG+AGG	+	chr6.2:76530103-76530122	MS.gene95951:intron	30.0%
TTTCTTTCACTTGATCATAC+TGG	-	chr6.2:76529890-76529909	None:intergenic	30.0%
AAGCATGAGAATATCAACTG+AGG	-	chr6.2:76529627-76529646	None:intergenic	35.0%
CAACGGTAAAACCATTTGTA+TGG	+	chr6.2:76529651-76529670	MS.gene95951:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	76529482	76530588	76529482	ID=MS.gene95951
chr6.2	mRNA	76529482	76530588	76529482	ID=MS.gene95951.t1;Parent=MS.gene95951
chr6.2	exon	76529482	76529655	76529482	ID=MS.gene95951.t1.exon1;Parent=MS.gene95951.t1
chr6.2	CDS	76529482	76529655	76529482	ID=cds.MS.gene95951.t1;Parent=MS.gene95951.t1
chr6.2	exon	76529801	76529987	76529801	ID=MS.gene95951.t1.exon2;Parent=MS.gene95951.t1
chr6.2	CDS	76529801	76529987	76529801	ID=cds.MS.gene95951.t1;Parent=MS.gene95951.t1
chr6.2	exon	76530108	76530197	76530108	ID=MS.gene95951.t1.exon3;Parent=MS.gene95951.t1
chr6.2	CDS	76530108	76530197	76530108	ID=cds.MS.gene95951.t1;Parent=MS.gene95951.t1
chr6.2	exon	76530476	76530588	76530476	ID=MS.gene95951.t1.exon4;Parent=MS.gene95951.t1
chr6.2	CDS	76530476	76530588	76530476	ID=cds.MS.gene95951.t1;Parent=MS.gene95951.t1

Gene Sequence

>MS.gene95951

ATGCCGCCGAAGAGGTCATCAAGAGGAAAACAAAAAGTGGAGCTAAAAAAAATTGAGAGTAAGAATGATCTCTTTGTTGCATTTACAACGAGAAAGTCTGGAATTTACAACAAGGCCTCTGAGTTAACAACACTTTCAGGGGCCTCAGTTGATATTCTCATGCTTTCTCCAACGGTAAAACCATTTGTATGGAAAACCATCTTCTGAATCTATAACAAAAACTAGTTTGAATGATGAAAATCCTTCAATAGAAGAGGAAATGATGAAACCGAAAATTATACAGCAAAATGAAAAAAATGATAATCTTTTGGATAAAAAGCATCCTGCGGAAGCACAACGTAAAAATTTGAAGATGTTGGAAAGATCTGGATATTGGTCAATTCAAGAGGAAGATGATAAAAGCTGCCAGTATGATCAAGTGAAAGAAATTGACAAATCCTTAGTTGTTGTCACAAATAAATTGATAAATAAAGTCTTGAAAAATGGAGGAGATCTTAACCCTCGTGGTATGTGTACATCTTCTAACTAACTATGTGTTATATTTGATCTATTAATGACGTCAAATGATTTCATGTCATCCTTTTTGTCTAATTATTTTAAATGAATCTGGTTATAATTTTTTGTAGTAATTTCTACTATCGAGGAACTGAATGGAGTCAAAACAAATCATGCTTATGTTTTTGAGGACAACAGAAAAGATCATGAAATTGGTGCAAGTAAGAATTCTCATTCCTGTTTTTAATTTTTTGTTTTTGCTTGAAATATTCTAGCGGTGACACTCACACATTAAACTTTTGGACAAGAGATGTTGTTGGTTAATCGTGTCCCAGCTTTTACCATTATAGTTTCACTTATGGGAAAAATTGCAATTACTTTTCTTCTTAATTTCTATTACTAGGCATTCAAACACTTACTTATAATATTTCATGCGAAAAATCTAACATATGTTATGTATTGTCTTCCTTTCATGGATTCAATCTTTTTGATCCAAAAGGTAAAGGTATGGTTGCTAATACTTCTTATGATATAGAAACACAAGGAGTCAACCTTATTGGTGCAAGCGGAGGATATGAAAGTGCTGTTGATCATGCTTCTCGAGATATATGA

Protein sequence

>MS.gene95951.t1

MPPKRSSRGKQKVELKKIESKNDLFVAFTTRKSGIYNKASELTTLSGASVDILMLSPTHPAEAQRKNLKMLERSGYWSIQEEDDKSCQYDQVKEIDKSLVVVTNKLINKVLKNGGDLNPRVISTIEELNGVKTNHAYVFEDNRKDHEIGASKGMVANTSYDIETQGVNLIGASGGYESAVDHASRDI