Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95951.t1 | XP_013455733.1 | 59.8 | 239 | 39 | 4 | 1 | 187 | 1 | 234 | 6.90E-60 | 240.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95951.t1 | Q42498 | 27.3 | 165 | 116 | 3 | 9 | 170 | 2 | 165 | 2.0e-07 | 57.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95951.t1 | A0A072UJ33 | 59.8 | 239 | 39 | 4 | 1 | 187 | 1 | 234 | 5.0e-60 | 240.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene95951.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95951.t1 | MTR_4g051538 | 61.506 | 239 | 35 | 4 | 1 | 187 | 1 | 234 | 1.20e-82 | 244 |
| MS.gene95951.t1 | MTR_3g104630 | 37.566 | 189 | 55 | 5 | 1 | 173 | 1 | 142 | 3.55e-27 | 100 |
| MS.gene95951.t1 | MTR_3g052920 | 52.830 | 53 | 25 | 0 | 5 | 57 | 5 | 57 | 1.15e-12 | 64.3 |
| MS.gene95951.t1 | MTR_8g043650 | 49.123 | 57 | 29 | 0 | 1 | 57 | 1 | 57 | 2.04e-12 | 63.9 |
| MS.gene95951.t1 | MTR_1g012570 | 50.943 | 53 | 26 | 0 | 5 | 57 | 5 | 57 | 2.19e-12 | 63.9 |
| MS.gene95951.t1 | MTR_3g052870 | 50.943 | 53 | 26 | 0 | 5 | 57 | 5 | 57 | 2.50e-12 | 63.2 |
| MS.gene95951.t1 | MTR_1g105920 | 50.943 | 53 | 26 | 0 | 5 | 57 | 5 | 57 | 3.55e-12 | 63.2 |
| MS.gene95951.t1 | MTR_1g105910 | 50.943 | 53 | 26 | 0 | 5 | 57 | 5 | 57 | 3.89e-12 | 63.2 |
| MS.gene95951.t1 | MTR_1g106070 | 49.123 | 57 | 29 | 0 | 1 | 57 | 1 | 57 | 4.81e-12 | 62.0 |
| MS.gene95951.t1 | MTR_2g030740 | 50.943 | 53 | 26 | 0 | 5 | 57 | 5 | 57 | 6.69e-12 | 62.4 |
| MS.gene95951.t1 | MTR_2g085250 | 44.444 | 54 | 30 | 0 | 5 | 58 | 5 | 58 | 2.64e-11 | 60.8 |
| MS.gene95951.t1 | MTR_2g085280 | 44.444 | 54 | 30 | 0 | 5 | 58 | 5 | 58 | 2.75e-11 | 60.8 |
| MS.gene95951.t1 | MTR_1g077360 | 44.444 | 54 | 30 | 0 | 5 | 58 | 5 | 58 | 5.16e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95951.t1 | AT4G36590 | 49.057 | 53 | 27 | 0 | 5 | 57 | 3 | 55 | 3.49e-12 | 63.5 |
Find 28 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGAGTTAACAACACTTTC+AGG | 0.202264 | 6.2:+76529599 | MS.gene95951:CDS |
| ACACAAGGAGTCAACCTTAT+TGG | 0.323352 | 6.2:+76530514 | MS.gene95951:CDS |
| AACAGAAAAGATCATGAAAT+TGG | 0.332742 | 6.2:+76530170 | MS.gene95951:CDS |
| ATCCTCCGCTTGCACCAATA+AGG | 0.357163 | 6.2:-76530528 | None:intergenic |
| TTGAAGATGTTGGAAAGATC+TGG | 0.393301 | 6.2:+76529828 | MS.gene95951:CDS |
| TTTCTTTCACTTGATCATAC+TGG | 0.398154 | 6.2:-76529887 | None:intergenic |
| TTTATTTGTGACAACAACTA+AGG | 0.433521 | 6.2:-76529919 | None:intergenic |
| TTTCCTCTTGATGACCTCTT+CGG | 0.445330 | 6.2:-76529488 | None:intergenic |
| GCATTTACAACGAGAAAGTC+TGG | 0.455029 | 6.2:+76529560 | MS.gene95951:CDS |
| TGTTGGAAAGATCTGGATAT+TGG | 0.465576 | 6.2:+76529835 | MS.gene95951:CDS |
| CTGAGTTAACAACACTTTCA+GGG | 0.482866 | 6.2:+76529600 | MS.gene95951:CDS |
| TATTCTCATGCTTTCTCCAA+CGG | 0.500320 | 6.2:+76529634 | MS.gene95951:CDS |
| TGATCCAAAAGGTAAAGGTA+TGG | 0.511773 | 6.2:+76530465 | MS.gene95951:intron |
| TGTAGTAATTTCTACTATCG+AGG | 0.554561 | 6.2:+76530103 | MS.gene95951:intron |
| TCTACTATCGAGGAACTGAA+TGG | 0.564056 | 6.2:+76530113 | MS.gene95951:CDS |
| GAGTAAGAATGCCGCCGAAG+AGG | 0.583357 | 6.2:+76529474 | None:intergenic |
| GTCAACCTTATTGGTGCAAG+CGG | 0.586884 | 6.2:+76530523 | MS.gene95951:CDS |
| AACCTTATTGGTGCAAGCGG+AGG | 0.596051 | 6.2:+76530526 | MS.gene95951:CDS |
| CCTCTTGATGACCTCTTCGG+CGG | 0.600952 | 6.2:-76529485 | None:intergenic |
| TGAGTTAACAACACTTTCAG+GGG | 0.607847 | 6.2:+76529601 | MS.gene95951:CDS |
| TGGATATTGGTCAATTCAAG+AGG | 0.615759 | 6.2:+76529848 | MS.gene95951:CDS |
| CCGCCGAAGAGGTCATCAAG+AGG | 0.664394 | 6.2:+76529485 | MS.gene95951:CDS |
| TGAAAGTGTTGTTAACTCAG+AGG | 0.669188 | 6.2:-76529597 | None:intergenic |
| GGAGGAGATCTTAACCCTCG+TGG | 0.672202 | 6.2:+76529966 | MS.gene95951:CDS |
| AAGCATGAGAATATCAACTG+AGG | 0.718686 | 6.2:-76529624 | None:intergenic |
| TCTTATGATATAGAAACACA+AGG | 0.732301 | 6.2:+76530499 | MS.gene95951:CDS |
| AAGATGTACACATACCACGA+GGG | 0.757349 | 6.2:-76529980 | None:intergenic |
| AAAGTCTGGAATTTACAACA+AGG | 0.757794 | 6.2:+76529574 | MS.gene95951:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACAAAAAATTAAAAAC+AGG | - | chr6.2:76530216-76530235 | None:intergenic | 10.0% |
| !!! | TTTAAAATAATTAGACAAAA+AGG | - | chr6.2:76530063-76530082 | None:intergenic | 10.0% |
| !! | ATAAATAAAGTCTTGAAAAA+TGG | + | chr6.2:76529945-76529964 | MS.gene95951:CDS | 15.0% |
| !!! | CTAATTATTTTAAATGAATC+TGG | + | chr6.2:76530069-76530088 | MS.gene95951:intron | 15.0% |
| !!! | TGAAAAAAATGATAATCTTT+TGG | + | chr6.2:76529770-76529789 | MS.gene95951:intron | 15.0% |
| !! | TCTTCTTAATTTCTATTACT+AGG | + | chr6.2:76530358-76530377 | MS.gene95951:intron | 20.0% |
| !!! | TCATTTTGCTGTATAATTTT+CGG | - | chr6.2:76529753-76529772 | None:intergenic | 20.0% |
| ! | AAAAAGATTGAATCCATGAA+AGG | - | chr6.2:76530446-76530465 | None:intergenic | 25.0% |
| ! | AAACTATAATGGTAAAAGCT+GGG | - | chr6.2:76530310-76530329 | None:intergenic | 25.0% |
| ! | AACAGAAAAGATCATGAAAT+TGG | + | chr6.2:76530170-76530189 | MS.gene95951:CDS | 25.0% |
| ! | AATAAAGTCTTGAAAAATGG+AGG | + | chr6.2:76529948-76529967 | MS.gene95951:CDS | 25.0% |
| ! | ACCATTATAGTTTCACTTAT+GGG | + | chr6.2:76530317-76530336 | MS.gene95951:intron | 25.0% |
| ! | ACGTAAAAATTTGAAGATGT+TGG | + | chr6.2:76529818-76529837 | MS.gene95951:CDS | 25.0% |
| ! | TACCATTATAGTTTCACTTA+TGG | + | chr6.2:76530316-76530335 | MS.gene95951:intron | 25.0% |
| ! | TCTTATGATATAGAAACACA+AGG | + | chr6.2:76530499-76530518 | MS.gene95951:CDS | 25.0% |
| ! | TTTATTTGTGACAACAACTA+AGG | - | chr6.2:76529922-76529941 | None:intergenic | 25.0% |
| !! | TTCAATCTTTTTGATCCAAA+AGG | + | chr6.2:76530454-76530473 | MS.gene95951:intron | 25.0% |
| !! | TTTGTTATAGATTCAGAAGA+TGG | - | chr6.2:76529681-76529700 | None:intergenic | 25.0% |
| !! | TTTTGCTTGAAATATTCTAG+CGG | + | chr6.2:76530232-76530251 | MS.gene95951:intron | 25.0% |
| !!! | AAATCATGCTTATGTTTTTG+AGG | + | chr6.2:76530145-76530164 | MS.gene95951:CDS | 25.0% |
| ATCAAGAGGAAAACAAAAAG+TGG | + | chr6.2:76529499-76529518 | MS.gene95951:CDS | 30.0% | |
| ATCATTTCCTCTTCTATTGA+AGG | - | chr6.2:76529726-76529745 | None:intergenic | 30.0% | |
| GAAACTATAATGGTAAAAGC+TGG | - | chr6.2:76530311-76530330 | None:intergenic | 30.0% | |
| TATGTATTGTCTTCCTTTCA+TGG | + | chr6.2:76530430-76530449 | MS.gene95951:intron | 30.0% | |
| TCCCATAAGTGAAACTATAA+TGG | - | chr6.2:76530321-76530340 | None:intergenic | 30.0% | |
| TGAAAATCCTTCAATAGAAG+AGG | + | chr6.2:76529716-76529735 | MS.gene95951:intron | 30.0% | |
| TGTAGTAATTTCTACTATCG+AGG | + | chr6.2:76530103-76530122 | MS.gene95951:intron | 30.0% | |
| TTTCTTTCACTTGATCATAC+TGG | - | chr6.2:76529890-76529909 | None:intergenic | 30.0% | |
| ! | AAAGTCTGGAATTTACAACA+AGG | + | chr6.2:76529574-76529593 | MS.gene95951:CDS | 30.0% |
| ! | AAGATGGTTTTCCATACAAA+TGG | - | chr6.2:76529665-76529684 | None:intergenic | 30.0% |
| ! | ACACTCACACATTAAACTTT+TGG | + | chr6.2:76530257-76530276 | MS.gene95951:intron | 30.0% |
| ! | CTTTTTGATCCAAAAGGTAA+AGG | + | chr6.2:76530460-76530479 | MS.gene95951:intron | 30.0% |
| AAGCATGAGAATATCAACTG+AGG | - | chr6.2:76529627-76529646 | None:intergenic | 35.0% | |
| CAACGGTAAAACCATTTGTA+TGG | + | chr6.2:76529651-76529670 | MS.gene95951:intron | 35.0% | |
| CTGAGTTAACAACACTTTCA+GGG | + | chr6.2:76529600-76529619 | MS.gene95951:CDS | 35.0% | |
| TATTCTCATGCTTTCTCCAA+CGG | + | chr6.2:76529634-76529653 | MS.gene95951:CDS | 35.0% | |
| TCTGAGTTAACAACACTTTC+AGG | + | chr6.2:76529599-76529618 | MS.gene95951:CDS | 35.0% | |
| TGAGTTAACAACACTTTCAG+GGG | + | chr6.2:76529601-76529620 | MS.gene95951:CDS | 35.0% | |
| TGATCCAAAAGGTAAAGGTA+TGG | + | chr6.2:76530465-76530484 | MS.gene95951:intron | 35.0% | |
| TGGATATTGGTCAATTCAAG+AGG | + | chr6.2:76529848-76529867 | MS.gene95951:CDS | 35.0% | |
| TGTTGGAAAGATCTGGATAT+TGG | + | chr6.2:76529835-76529854 | MS.gene95951:CDS | 35.0% | |
| TTGAAGATGTTGGAAAGATC+TGG | + | chr6.2:76529828-76529847 | MS.gene95951:CDS | 35.0% | |
| ! | TTTTGGACAAGAGATGTTGT+TGG | + | chr6.2:76530274-76530293 | MS.gene95951:intron | 35.0% |
| !! | CATACAAATGGTTTTACCGT+TGG | - | chr6.2:76529653-76529672 | None:intergenic | 35.0% |
| !! | TGAAAGTGTTGTTAACTCAG+AGG | - | chr6.2:76529600-76529619 | None:intergenic | 35.0% |
| !! | TTTGGATAAAAAGCATCCTG+CGG | + | chr6.2:76529788-76529807 | MS.gene95951:intron | 35.0% |
| AAGATGTACACATACCACGA+GGG | - | chr6.2:76529983-76530002 | None:intergenic | 40.0% | |
| ACACAAGGAGTCAACCTTAT+TGG | + | chr6.2:76530514-76530533 | MS.gene95951:CDS | 40.0% | |
| GCATTTACAACGAGAAAGTC+TGG | + | chr6.2:76529560-76529579 | MS.gene95951:CDS | 40.0% | |
| TCTACTATCGAGGAACTGAA+TGG | + | chr6.2:76530113-76530132 | MS.gene95951:CDS | 40.0% | |
| ! | GCAACCATACCTTTACCTTT+TGG | - | chr6.2:76530472-76530491 | None:intergenic | 40.0% |
| ! | TTTCCTCTTGATGACCTCTT+CGG | - | chr6.2:76529491-76529510 | None:intergenic | 40.0% |
| GAAGATGTACACATACCACG+AGG | - | chr6.2:76529984-76530003 | None:intergenic | 45.0% | |
| GTCAACCTTATTGGTGCAAG+CGG | + | chr6.2:76530523-76530542 | MS.gene95951:CDS | 45.0% | |
| ! | TTTTTACGTTGTGCTTCCGC+AGG | - | chr6.2:76529807-76529826 | None:intergenic | 45.0% |
| AACCTTATTGGTGCAAGCGG+AGG | + | chr6.2:76530526-76530545 | MS.gene95951:CDS | 50.0% | |
| ATCCTCCGCTTGCACCAATA+AGG | - | chr6.2:76530531-76530550 | None:intergenic | 50.0% | |
| GGAGGAGATCTTAACCCTCG+TGG | + | chr6.2:76529966-76529985 | MS.gene95951:CDS | 55.0% | |
| ! | CCTCTTGATGACCTCTTCGG+CGG | - | chr6.2:76529488-76529507 | None:intergenic | 55.0% |
| CCGCCGAAGAGGTCATCAAG+AGG | + | chr6.2:76529485-76529504 | MS.gene95951:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 76529482 | 76530588 | 76529482 | ID=MS.gene95951 |
| chr6.2 | mRNA | 76529482 | 76530588 | 76529482 | ID=MS.gene95951.t1;Parent=MS.gene95951 |
| chr6.2 | exon | 76529482 | 76529655 | 76529482 | ID=MS.gene95951.t1.exon1;Parent=MS.gene95951.t1 |
| chr6.2 | CDS | 76529482 | 76529655 | 76529482 | ID=cds.MS.gene95951.t1;Parent=MS.gene95951.t1 |
| chr6.2 | exon | 76529801 | 76529987 | 76529801 | ID=MS.gene95951.t1.exon2;Parent=MS.gene95951.t1 |
| chr6.2 | CDS | 76529801 | 76529987 | 76529801 | ID=cds.MS.gene95951.t1;Parent=MS.gene95951.t1 |
| chr6.2 | exon | 76530108 | 76530197 | 76530108 | ID=MS.gene95951.t1.exon3;Parent=MS.gene95951.t1 |
| chr6.2 | CDS | 76530108 | 76530197 | 76530108 | ID=cds.MS.gene95951.t1;Parent=MS.gene95951.t1 |
| chr6.2 | exon | 76530476 | 76530588 | 76530476 | ID=MS.gene95951.t1.exon4;Parent=MS.gene95951.t1 |
| chr6.2 | CDS | 76530476 | 76530588 | 76530476 | ID=cds.MS.gene95951.t1;Parent=MS.gene95951.t1 |
| Gene Sequence |
| Protein sequence |
