AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96098

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96098.t1 MTR_7g075230 96.954 197 6 0 1 197 1 197 2.16e-142 394
MS.gene96098.t1 MTR_8g036105 62.500 200 60 6 4 197 10 200 5.86e-70 212
MS.gene96098.t1 MTR_8g036085 72.642 106 27 2 11 116 22 125 6.07e-52 167
MS.gene96098.t1 MTR_7g074990 67.619 105 32 2 12 116 20 122 4.32e-46 152
MS.gene96098.t1 MTR_7g096610 65.546 119 36 4 10 125 6 122 2.04e-45 150
MS.gene96098.t1 MTR_1g070205 66.337 101 32 2 12 112 12 110 1.75e-44 148
MS.gene96098.t1 MTR_7g096530 65.487 113 37 2 4 116 6 116 3.34e-44 146
MS.gene96098.t1 MTR_1g070220 58.065 124 50 2 10 133 13 134 1.04e-41 140
MS.gene96098.t1 MTR_6g027710 62.385 109 37 3 12 120 40 144 1.00e-39 135
MS.gene96098.t1 MTR_5g080470 61.616 99 36 2 10 108 5 101 3.22e-39 133
MS.gene96098.t1 MTR_5g083230 51.938 129 55 3 9 133 3 128 2.74e-36 125
MS.gene96098.t1 MTR_3g071590 48.092 131 65 2 6 136 2 129 6.17e-35 121
MS.gene96098.t1 MTR_7g033800 53.043 115 51 2 12 126 11 122 1.07e-32 115
MS.gene96098.t1 MTR_2g068760 52.632 114 45 3 8 115 3 113 1.56e-32 115
MS.gene96098.t1 MTR_3g031660 51.402 107 49 2 9 115 4 107 4.81e-32 114
MS.gene96098.t1 MTR_4g060950 42.208 154 72 4 11 150 23 173 1.36e-31 113
MS.gene96098.t1 MTR_4g105170 58.586 99 38 2 10 108 9 104 1.63e-31 113
MS.gene96098.t1 MTR_3g077240 55.660 106 44 2 10 115 8 110 3.10e-31 112
MS.gene96098.t1 MTR_6g005070 53.704 108 47 2 12 119 9 113 4.02e-31 110
MS.gene96098.t1 MTR_4g083680 54.902 102 43 2 12 113 15 113 5.06e-31 111
MS.gene96098.t1 MTR_6g027700 50.893 112 53 2 8 119 3 112 1.23e-30 111
MS.gene96098.t1 MTR_5g083010 51.923 104 47 2 13 116 12 112 2.56e-30 110
MS.gene96098.t1 MTR_5g083960 42.038 157 81 4 12 162 39 191 7.75e-29 106
MS.gene96098.t1 MTR_2g093310 49.524 105 50 2 11 115 32 133 8.98e-29 106
MS.gene96098.t1 MTR_3g452660 52.778 108 48 2 12 119 7 111 1.98e-28 107
MS.gene96098.t1 MTR_6g005080 51.923 104 47 2 12 115 10 110 2.37e-28 103
MS.gene96098.t1 MTR_3g073690 50.926 108 50 2 9 116 6 110 6.24e-28 104
MS.gene96098.t1 MTR_3g071420 50.000 100 47 2 13 112 15 111 2.04e-27 102
MS.gene96098.t1 MTR_5g017950 52.381 105 47 2 12 116 8 109 3.99e-27 105
MS.gene96098.t1 MTR_1g095850 50.485 103 48 2 13 115 73 172 1.34e-26 101
MS.gene96098.t1 MTR_3g094690 50.962 104 48 2 12 115 47 147 1.52e-26 101
MS.gene96098.t1 MTR_3g094690 50.962 104 47 3 12 115 47 146 7.54e-25 97.4
MS.gene96098.t1 MTR_5g075020 53.846 104 45 2 12 115 43 143 8.28e-23 92.0
MS.gene96098.t1 MTR_5g075020 53.846 104 45 2 12 115 47 147 1.32e-22 91.3
MS.gene96098.t1 MTR_5g093010 42.453 106 58 2 11 116 4 106 2.39e-22 88.6
MS.gene96098.t1 MTR_8g040900 49.550 111 53 2 12 122 7 114 1.88e-21 88.2
MS.gene96098.t1 MTR_7g028905 38.462 104 61 2 13 116 6 106 3.74e-20 82.0
MS.gene96098.t1 MTR_8g079660 39.048 105 61 2 12 116 8 109 1.41e-17 78.6
MS.gene96098.t1 MTR_8g079620 40.952 105 59 2 12 116 8 109 1.77e-16 75.1
MS.gene96098.t1 MTR_2g100020 37.143 105 63 2 13 117 20 121 1.08e-15 73.6
MS.gene96098.t1 MTR_8g017090 37.736 106 63 2 13 118 13 115 3.28e-15 72.8
MS.gene96098.t1 MTR_6g011250 39.623 106 60 3 13 117 10 112 3.97e-15 70.5
MS.gene96098.t1 MTR_8g079580 36.538 104 64 1 12 115 8 109 9.06e-15 70.5
MS.gene96098.t1 MTR_4g107450 37.143 105 63 2 13 117 11 112 5.25e-14 69.3
MS.gene96098.t1 MTR_6g011230 33.929 112 70 3 1 112 1 108 2.39e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96098.t1 AT2G45410 71.429 119 31 3 15 132 19 135 5.94e-53 168
MS.gene96098.t1 AT4G00210 63.971 136 43 3 10 141 9 142 2.09e-52 167
MS.gene96098.t1 AT2G45420 75.238 105 24 2 15 119 40 142 1.98e-48 159
MS.gene96098.t1 AT4G00220 69.524 105 30 2 15 119 20 122 1.36e-45 150
MS.gene96098.t1 AT3G03760 68.468 111 32 3 12 122 51 158 5.55e-45 150
MS.gene96098.t1 AT3G58190 57.851 121 49 2 12 132 11 129 1.06e-44 148
MS.gene96098.t1 AT2G42430 56.934 137 48 4 10 137 13 147 6.56e-44 147
MS.gene96098.t1 AT2G42440 55.200 125 49 3 10 129 5 127 1.51e-42 143
MS.gene96098.t1 AT5G06080 48.026 152 64 4 10 151 5 151 4.23e-40 135
MS.gene96098.t1 AT2G31310 58.879 107 42 2 10 116 5 109 1.97e-39 133
MS.gene96098.t1 AT2G30130 50.407 123 57 3 6 128 2 120 1.02e-33 119
MS.gene96098.t1 AT5G63090 57.143 105 42 2 12 116 11 112 2.10e-31 113
MS.gene96098.t1 AT5G63090 57.143 105 42 2 12 116 11 112 2.10e-31 113
MS.gene96098.t1 AT5G63090 57.143 105 42 2 12 116 11 112 2.10e-31 113
MS.gene96098.t1 AT5G63090 57.143 105 42 2 12 116 11 112 2.10e-31 113
MS.gene96098.t1 AT1G16530 49.153 118 55 2 11 125 13 128 2.75e-31 112
MS.gene96098.t1 AT3G27650 53.846 104 45 2 12 115 39 139 3.07e-30 109
MS.gene96098.t1 AT1G07900 40.741 162 82 5 11 164 32 187 5.03e-30 109
MS.gene96098.t1 AT3G11090 40.260 154 86 3 13 163 12 162 1.53e-29 107
MS.gene96098.t1 AT2G28500 50.962 104 48 2 12 115 55 155 9.84e-29 107
MS.gene96098.t1 AT1G31320 53.333 90 39 2 12 101 13 99 8.94e-28 103
MS.gene96098.t1 AT5G66870 49.635 137 54 5 12 136 7 140 9.20e-28 106
MS.gene96098.t1 AT3G26660 44.762 105 55 2 13 117 6 107 2.71e-27 100
MS.gene96098.t1 AT3G26620 44.762 105 55 2 13 117 6 107 4.22e-27 100
MS.gene96098.t1 AT2G23660 47.115 104 52 2 12 115 5 105 5.36e-25 99.4
MS.gene96098.t1 AT2G23660 47.115 104 52 2 12 115 5 105 5.36e-25 99.4
MS.gene96098.t1 AT2G23660 47.115 104 52 2 12 115 5 105 5.36e-25 99.4
MS.gene96098.t1 AT2G30340 48.077 104 51 2 12 115 52 152 1.22e-24 97.4
MS.gene96098.t1 AT2G30340 48.077 104 51 2 12 115 53 153 1.29e-24 97.4
MS.gene96098.t1 AT3G50510 43.636 110 59 2 7 116 7 113 4.24e-23 92.0
MS.gene96098.t1 AT3G50510 43.636 110 59 2 7 116 7 113 4.24e-23 92.0
MS.gene96098.t1 AT2G40470 52.885 104 46 2 12 115 20 120 5.94e-22 89.0
MS.gene96098.t1 AT2G40470 52.885 104 46 2 12 115 45 145 6.72e-22 89.4
MS.gene96098.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.58e-22 88.6
MS.gene96098.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.58e-22 88.6
MS.gene96098.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.58e-22 88.6
MS.gene96098.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.58e-22 88.6
MS.gene96098.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.58e-22 88.6
MS.gene96098.t1 AT3G13850 31.902 163 98 5 13 168 37 193 1.34e-15 73.2
MS.gene96098.t1 AT1G72980 35.772 123 74 3 9 129 8 127 1.93e-15 72.0
MS.gene96098.t1 AT5G35900 33.043 115 74 2 13 127 6 117 4.51e-15 70.9
MS.gene96098.t1 AT1G06280 41.558 77 42 2 13 89 25 98 2.75e-12 63.2
MS.gene96098.t1 AT3G47870 29.907 107 72 2 11 117 35 138 3.53e-12 64.3

Find 41 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGAGAGAGAGATGGATTT+GGG 0.204949 7.4:+30185103 None:intergenic
AGGGCTACTGACCCTGTTTA+TGG 0.239262 7.4:+30185358 MS.gene96098:CDS
TTTAGAGAGAGAGATGGATT+TGG 0.290229 7.4:+30185102 None:intergenic
TAGGCAAAGCTTGGAATTCC+TGG 0.335459 7.4:-30187983 None:intergenic
GGGTGTGGGGATGACGGTGG+TGG 0.353668 7.4:+30185124 MS.gene96098:CDS
AGAGAGATGGATTTGGGGTG+TGG 0.373011 7.4:+30185109 None:intergenic
TGACCTGATGACTCAAAATA+TGG 0.381006 7.4:-30185208 None:intergenic
CTTTGAGGCTGTTGAAATTG+AGG 0.406522 7.4:-30187795 None:intergenic
TGAGCAACACAACCATAAAC+AGG 0.407283 7.4:-30185370 None:intergenic
GAGAGATGGATTTGGGGTGT+GGG 0.427932 7.4:+30185110 None:intergenic
GCTGCAGTTCATAAGGTGTT+TGG 0.429613 7.4:+30185247 MS.gene96098:CDS
GTCATGAAGTTGTTGATAAT+AGG 0.429901 7.4:-30187961 None:intergenic
TTGGGGTGTGGGGATGACGG+TGG 0.437721 7.4:+30185121 MS.gene96098:CDS
CCTCTTTGCTCTTCAACAAC+AGG 0.440953 7.4:+30185393 MS.gene96098:CDS
GCATCAAGACGCTTATGAAC+TGG 0.441293 7.4:-30185304 None:intergenic
GATTTGGGGTGTGGGGATGA+CGG 0.456260 7.4:+30185118 MS.gene96098:CDS
GTGAGGATGAATGCAAAGTT+GGG 0.461415 7.4:-30187830 None:intergenic
GCGATTCAATATTGAAAGAC+GGG 0.465789 7.4:-30187760 None:intergenic
AGTGAGGATGAATGCAAAGT+TGG 0.469384 7.4:-30187831 None:intergenic
AAAATTCTCTAGGCAAAGCT+TGG 0.490828 7.4:-30187992 None:intergenic
GGGGATATTGAAGAGCTTTG+AGG 0.494424 7.4:-30187810 None:intergenic
TCTGCTATGAGGCTCTTGCT+AGG 0.495453 7.4:+30185338 MS.gene96098:CDS
CTGCTATGAGGCTCTTGCTA+GGG 0.502068 7.4:+30185339 MS.gene96098:CDS
TCTTACATGTCCATGCACTT+TGG 0.510678 7.4:+30187882 MS.gene96098:CDS
GGTGTGGGGATGACGGTGGT+GGG 0.515639 7.4:+30185125 MS.gene96098:CDS
ATTGAAAGCTAAAATTCTCT+AGG 0.516519 7.4:-30188002 None:intergenic
TGAAAGACGGGCTTGTACAT+AGG 0.516839 7.4:-30187748 None:intergenic
TGATAATAGGAATAGTATGA+TGG 0.524273 7.4:-30187948 None:intergenic
TGTTGTTACTCTCTGCTATG+AGG 0.534536 7.4:+30185327 MS.gene96098:CDS
TAGAGAGAGAGATGGATTTG+GGG 0.538982 7.4:+30185104 None:intergenic
AGAGATGGATTTGGGGTGTG+GGG 0.541784 7.4:+30185111 None:intergenic
TTGAGAAGAAAGTGTGTGAA+AGG 0.553888 7.4:+30185169 MS.gene96098:CDS
TGCGATTCAATATTGAAAGA+CGG 0.566372 7.4:-30187761 None:intergenic
GATGACGGTGGTGGGCCTTG+TGG 0.575954 7.4:+30185133 MS.gene96098:CDS
AAGAGGATGACCAAAGTGCA+TGG 0.603188 7.4:-30187892 None:intergenic
TGAGGATGAATGCAAAGTTG+GGG 0.614688 7.4:-30187829 None:intergenic
GGATGATATTGAAGTTGAAG+AGG 0.622419 7.4:-30187909 None:intergenic
TTATCAACAACTTCATGACC+AGG 0.628553 7.4:+30187965 MS.gene96098:CDS
CCTGTTGTTGAAGAGCAAAG+AGG 0.633272 7.4:-30185393 None:intergenic
TGATGACAAATGATGAAGTG+AGG 0.675536 7.4:-30187847 None:intergenic
GAGCAACACAACCATAAACA+GGG 0.707824 7.4:-30185369 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATGACATATATAAAT+AGG - chr7.4:30185762-30185781 None:intergenic 10.0%
!! TAGAAAAAAAAAAAAATGTT+TGG - chr7.4:30186538-30186557 None:intergenic 10.0%
!! TTATATACTTAATTAATTAC+AGG + chr7.4:30187701-30187720 MS.gene96098:intron 10.0%
!!! AAATAGGATTTATATTATTT+TGG - chr7.4:30185746-30185765 None:intergenic 10.0%
!!! ATTTTATCAATAATATTCTT+TGG + chr7.4:30185792-30185811 MS.gene96098:intron 10.0%
!!! TATTATTTTGGAAAATAAAT+AGG - chr7.4:30185734-30185753 None:intergenic 10.0%
!! ATTCAAGAAAAACTTATAAT+AGG + chr7.4:30185611-30185630 MS.gene96098:intron 15.0%
!! CAGAAAAAAAGAAAAAAAAA+AGG + chr7.4:30185934-30185953 MS.gene96098:intron 15.0%
!! GTTAATATTATAATATTCCA+AGG - chr7.4:30186516-30186535 None:intergenic 15.0%
!! TAAATAGGATTTATATTGTT+TGG - chr7.4:30185719-30185738 None:intergenic 15.0%
!! TAATTCGTATATATATAACT+TGG + chr7.4:30185491-30185510 MS.gene96098:intron 15.0%
!! TATATATATACGAATTAGTT+AGG - chr7.4:30185488-30185507 None:intergenic 15.0%
!!! AGTTTATTAAATTTTATTCC+CGG + chr7.4:30186382-30186401 MS.gene96098:intron 15.0%
!!! ATGCAATGTAAAATAAATTT+TGG - chr7.4:30186167-30186186 None:intergenic 15.0%
!!! CTTTTTTTTTTCTTTTTTTC+TGG - chr7.4:30185936-30185955 None:intergenic 15.0%
!!! TTTTTTTTTTCTAAATACCT+TGG + chr7.4:30186545-30186564 MS.gene96098:intron 15.0%
!! AAAGACAATCTCTAAATTAT+TGG + chr7.4:30187484-30187503 MS.gene96098:intron 20.0%
!! AAATGTTAATAGTACCTTTA+GGG + chr7.4:30186857-30186876 MS.gene96098:intron 20.0%
!! AATAAACTTGTAAGAAACAT+AGG - chr7.4:30186370-30186389 None:intergenic 20.0%
!! AATTAAATAAGTATCCTAAC+TGG + chr7.4:30185880-30185899 MS.gene96098:intron 20.0%
!! AGAAGAATGGTTTAATAAAT+TGG + chr7.4:30185816-30185835 MS.gene96098:intron 20.0%
!! AGTCACACAAAAAAAAAAAA+AGG + chr7.4:30186833-30186852 MS.gene96098:intron 20.0%
!! AGTGTAACAAATTTATTCTT+AGG - chr7.4:30186609-30186628 None:intergenic 20.0%
!! ATAATTGTATTTAGACTACT+AGG - chr7.4:30187685-30187704 None:intergenic 20.0%
!! ATACCATATAAATGATCAAT+AGG - chr7.4:30187645-30187664 None:intergenic 20.0%
!! ATATAAATGATCAATAGGTT+AGG - chr7.4:30187640-30187659 None:intergenic 20.0%
!! ATGACTTACATAATGTTATT+TGG - chr7.4:30186991-30187010 None:intergenic 20.0%
!! ATTCATATCTTTATATTCCA+AGG + chr7.4:30186574-30186593 MS.gene96098:intron 20.0%
!! GTCACACAAAAAAAAAAAAA+GGG + chr7.4:30186834-30186853 MS.gene96098:intron 20.0%
!! TAACCTATTGATCATTTATA+TGG + chr7.4:30187639-30187658 MS.gene96098:intron 20.0%
!! TATAAATGATCAATAGGTTA+GGG - chr7.4:30187639-30187658 None:intergenic 20.0%
!! TGTATAAATACAACTATGTT+AGG - chr7.4:30187211-30187230 None:intergenic 20.0%
!! TTGATCAATATTAGTGTTTA+GGG - chr7.4:30187603-30187622 None:intergenic 20.0%
!! TTTCTAATTCACAAAAGATA+AGG - chr7.4:30187283-30187302 None:intergenic 20.0%
!!! AAGATATGAATTTTATTCCA+AGG - chr7.4:30186565-30186584 None:intergenic 20.0%
!!! ATTTTTATGATTTTATTCCC+AGG - chr7.4:30186340-30186359 None:intergenic 20.0%
!!! GTTTATTAAATTTTATTCCC+GGG + chr7.4:30186383-30186402 MS.gene96098:intron 20.0%
!!! TATATTGCTTTAATTTGTTC+AGG - chr7.4:30186041-30186060 None:intergenic 20.0%
!!! TCAAAGGCATAATATAATTA+AGG + chr7.4:30186657-30186676 MS.gene96098:intron 20.0%
!!! TGACTTACATAATGTTATTT+GGG - chr7.4:30186990-30187009 None:intergenic 20.0%
!!! TGTGTAATTTCCTATTTTTA+AGG + chr7.4:30186488-30186507 MS.gene96098:intron 20.0%
! AAGTTATAATTGTAGATTCC+TGG + chr7.4:30186319-30186338 MS.gene96098:intron 25.0%
! AATATTCTTTGGTAGAAGAA+TGG + chr7.4:30185803-30185822 MS.gene96098:intron 25.0%
! AATTTGTACATTCAAAGCAT+TGG + chr7.4:30186917-30186936 MS.gene96098:intron 25.0%
! ACAAAAGATAAGGTAACAAA+CGG - chr7.4:30187273-30187292 None:intergenic 25.0%
! ACTTAATTAATTACAGGTTG+TGG + chr7.4:30187707-30187726 MS.gene96098:intron 25.0%
! AGAAGAAAGATCATATTCAT+AGG + chr7.4:30185979-30185998 MS.gene96098:intron 25.0%
! AGTTATAATTGTAGATTCCT+GGG + chr7.4:30186320-30186339 MS.gene96098:intron 25.0%
! ATTTCACTTCATTAAAGAGT+GGG - chr7.4:30187097-30187116 None:intergenic 25.0%
! CTAATACATTACACAACAAT+GGG + chr7.4:30187555-30187574 MS.gene96098:intron 25.0%
! CTTGATCAATATTAGTGTTT+AGG - chr7.4:30187604-30187623 None:intergenic 25.0%
! GAAATGTTAATAGTACCTTT+AGG + chr7.4:30186856-30186875 MS.gene96098:intron 25.0%
! GAAGAAAGATCATATTCATA+GGG + chr7.4:30185980-30185999 MS.gene96098:intron 25.0%
! TAATACATTACACAACAATG+GGG + chr7.4:30187556-30187575 MS.gene96098:intron 25.0%
! TCTAAAGAAAATTACCAGTT+AGG - chr7.4:30185897-30185916 None:intergenic 25.0%
! TGATAATAGGAATAGTATGA+TGG - chr7.4:30187951-30187970 None:intergenic 25.0%
! TGTATTAGCCATGAAATTAA+AGG - chr7.4:30187543-30187562 None:intergenic 25.0%
!! AAAAGTTTTCCCAAAGAAAA+TGG + chr7.4:30186428-30186447 MS.gene96098:intron 25.0%
!! ACAAATGACCTTTAATTTCA+TGG + chr7.4:30187532-30187551 MS.gene96098:intron 25.0%
!! TTATTCTCACCATTTTCTTT+GGG - chr7.4:30186440-30186459 None:intergenic 25.0%
!! TTTATTCTCACCATTTTCTT+TGG - chr7.4:30186441-30186460 None:intergenic 25.0%
!!! TAGTATGATGGATTTTCAAA+AGG - chr7.4:30187939-30187958 None:intergenic 25.0%
!!! TGCTTTTGATACTTTAAAGA+TGG + chr7.4:30187122-30187141 MS.gene96098:intron 25.0%
AATGTATATTCTCAAGGTGT+TGG + chr7.4:30185647-30185666 MS.gene96098:intron 30.0%
AGGCAGAAAAATGAAAACTA+AGG - chr7.4:30185468-30185487 None:intergenic 30.0%
CAAAAGATAAGGTAACAAAC+GGG - chr7.4:30187272-30187291 None:intergenic 30.0%
CATCATATTCCAATATTGTG+TGG - chr7.4:30187028-30187047 None:intergenic 30.0%
CCACTAAAAGGAAACTAATA+AGG - chr7.4:30185686-30185705 None:intergenic 30.0%
GATTTCACTTCATTAAAGAG+TGG - chr7.4:30187098-30187117 None:intergenic 30.0%
GCTAATACATTACACAACAA+TGG + chr7.4:30187554-30187573 MS.gene96098:intron 30.0%
TGCGATTCAATATTGAAAGA+CGG - chr7.4:30187764-30187783 None:intergenic 30.0%
TGTATTGAGTATGTTCTGTT+GGG + chr7.4:30186192-30186211 MS.gene96098:intron 30.0%
TTCCAAGGAACCTTAAAAAT+AGG - chr7.4:30186501-30186520 None:intergenic 30.0%
TTCTTAGGAATAACATTCCT+TGG - chr7.4:30186594-30186613 None:intergenic 30.0%
TTGTATTGAGTATGTTCTGT+TGG + chr7.4:30186191-30186210 MS.gene96098:intron 30.0%
! AATATTGGAATATGATGACG+AGG + chr7.4:30187031-30187050 MS.gene96098:intron 30.0%
! AATTTTATTCCCATCTCCAT+GGG - chr7.4:30186467-30186486 None:intergenic 30.0%
! ATATTGGAATATGATGACGA+GGG + chr7.4:30187032-30187051 MS.gene96098:intron 30.0%
! ATTAGTGTTTAGGGTAATGT+TGG - chr7.4:30187594-30187613 None:intergenic 30.0%
! TAATTTTATTCCCATCTCCA+TGG - chr7.4:30186468-30186487 None:intergenic 30.0%
!! CCTTATTAGTTTCCTTTTAG+TGG + chr7.4:30185683-30185702 MS.gene96098:intron 30.0%
!! GTCATGAAGTTGTTGATAAT+AGG - chr7.4:30187964-30187983 None:intergenic 30.0%
!! TAACTTGGTGCTTTAAAAAC+TGG + chr7.4:30185506-30185525 MS.gene96098:intron 30.0%
!!! TGTGTACTTTTCTAAAGTAG+TGG + chr7.4:30186099-30186118 MS.gene96098:intron 30.0%
!!! TTCCTATTTTTAAGGTTCCT+TGG + chr7.4:30186496-30186515 MS.gene96098:intron 30.0%
AAAATTCTCTAGGCAAAGCT+TGG - chr7.4:30187995-30188014 None:intergenic 35.0%
AAGTGTCACAACATAGTGTT+AGG + chr7.4:30187245-30187264 MS.gene96098:intron 35.0%
AAGTTTCATGGTAAGTCTAG+TGG - chr7.4:30186728-30186747 None:intergenic 35.0%
AATAAAGTTCCCATGGAGAT+GGG + chr7.4:30186455-30186474 MS.gene96098:intron 35.0%
AGTTTCATGGTAAGTCTAGT+GGG - chr7.4:30186727-30186746 None:intergenic 35.0%
ATGTGAGGAACTAAGTTTCA+TGG - chr7.4:30186740-30186759 None:intergenic 35.0%
GCAGCTAATGTATATTCTCA+AGG + chr7.4:30185641-30185660 MS.gene96098:intron 35.0%
GCGATTCAATATTGAAAGAC+GGG - chr7.4:30187763-30187782 None:intergenic 35.0%
GGATGATATTGAAGTTGAAG+AGG - chr7.4:30187912-30187931 None:intergenic 35.0%
GTAATCATCACATGAATGTG+AGG - chr7.4:30186755-30186774 None:intergenic 35.0%
GTGTTTGATCGTGCATTTAA+TGG - chr7.4:30186684-30186703 None:intergenic 35.0%
TCATGTCAACCACACAATAT+TGG + chr7.4:30187016-30187035 MS.gene96098:intron 35.0%
TGACCTGATGACTCAAAATA+TGG - chr7.4:30185211-30185230 None:intergenic 35.0%
TGATGACAAATGATGAAGTG+AGG - chr7.4:30187850-30187869 None:intergenic 35.0%
TTATCAACAACTTCATGACC+AGG + chr7.4:30187965-30187984 MS.gene96098:CDS 35.0%
! AAGGTATAAGAGTATGAGAG+CGG + chr7.4:30185953-30185972 MS.gene96098:intron 35.0%
! GGGAAAACTTTTCTATTTCC+AGG - chr7.4:30186420-30186439 None:intergenic 35.0%
! TCATTTTGCTGCAGTTCATA+AGG + chr7.4:30185240-30185259 MS.gene96098:CDS 35.0%
! TTCCAGGAACATTTTATACC+CGG - chr7.4:30186404-30186423 None:intergenic 35.0%
! TTGAGAAGAAAGTGTGTGAA+AGG + chr7.4:30185169-30185188 MS.gene96098:CDS 35.0%
!! AGCTCTGGTTAGGTTTTATA+TGG + chr7.4:30186230-30186249 MS.gene96098:intron 35.0%
!! GATGGATTTTCAAAAGGTGT+TGG - chr7.4:30187933-30187952 None:intergenic 35.0%
!! TATTTTGAGTCATCAGGTCA+AGG + chr7.4:30185211-30185230 MS.gene96098:CDS 35.0%
AAAAACTTGCAAGCACCACA+AGG - chr7.4:30185151-30185170 None:intergenic 40.0%
AAAACTGGTAACCACTAACC+TGG + chr7.4:30185521-30185540 MS.gene96098:intron 40.0%
ACCCATGTCTTTGATCTCAA+AGG + chr7.4:30186641-30186660 MS.gene96098:intron 40.0%
AGTGAGGATGAATGCAAAGT+TGG - chr7.4:30187834-30187853 None:intergenic 40.0%
GAATAAAGTTCCCATGGAGA+TGG + chr7.4:30186454-30186473 MS.gene96098:intron 40.0%
GAGCAACACAACCATAAACA+GGG - chr7.4:30185372-30185391 None:intergenic 40.0%
GGACAAAAGAGACCACTAAA+AGG - chr7.4:30185698-30185717 None:intergenic 40.0%
GTGAGGATGAATGCAAAGTT+GGG - chr7.4:30187833-30187852 None:intergenic 40.0%
GTTTCATGGTAAGTCTAGTG+GGG - chr7.4:30186726-30186745 None:intergenic 40.0%
TAAAGAGTGGGTGATAGAGT+GGG - chr7.4:30187085-30187104 None:intergenic 40.0%
TCTTACATGTCCATGCACTT+TGG + chr7.4:30187882-30187901 MS.gene96098:CDS 40.0%
TGAGCAACACAACCATAAAC+AGG - chr7.4:30185373-30185392 None:intergenic 40.0%
TGAGGATGAATGCAAAGTTG+GGG - chr7.4:30187832-30187851 None:intergenic 40.0%
TGGAACGAATGCATACACTA+AGG + chr7.4:30186250-30186269 MS.gene96098:intron 40.0%
TGGTGAGAATAAAGTTCCCA+TGG + chr7.4:30186448-30186467 MS.gene96098:intron 40.0%
TGTTGTTACTCTCTGCTATG+AGG + chr7.4:30185327-30185346 MS.gene96098:CDS 40.0%
TTAAAGAGTGGGTGATAGAG+TGG - chr7.4:30187086-30187105 None:intergenic 40.0%
TTTGACACATTTGTCAGCTC+TGG + chr7.4:30186215-30186234 MS.gene96098:intron 40.0%
! AGGTATAAGAGTATGAGAGC+GGG + chr7.4:30185954-30185973 MS.gene96098:intron 40.0%
! TCCAGGAACATTTTATACCC+GGG - chr7.4:30186403-30186422 None:intergenic 40.0%
!! ATCCTTAAAGAGTGCCCTAA+AGG - chr7.4:30186874-30186893 None:intergenic 40.0%
!! CTTTGAGGCTGTTGAAATTG+AGG - chr7.4:30187798-30187817 None:intergenic 40.0%
!! GCACCATATTTTGAGTCATC+AGG + chr7.4:30185205-30185224 MS.gene96098:CDS 40.0%
!! GCCTTTGAGATCAAAGACAT+GGG - chr7.4:30186645-30186664 None:intergenic 40.0%
!! TACCTTTAGGGCACTCTTTA+AGG + chr7.4:30186869-30186888 MS.gene96098:intron 40.0%
!! TGCCTTTGAGATCAAAGACA+TGG - chr7.4:30186646-30186665 None:intergenic 40.0%
!! TTATGAACTTGTTACCCTCC+AGG - chr7.4:30185542-30185561 None:intergenic 40.0%
AAGAGGATGACCAAAGTGCA+TGG - chr7.4:30187895-30187914 None:intergenic 45.0%
ACATAACACTACCGTGCAAG+GGG - chr7.4:30186280-30186299 None:intergenic 45.0%
CACATAACACTACCGTGCAA+GGG - chr7.4:30186281-30186300 None:intergenic 45.0%
CACGGTAGTGTTATGTGCAT+GGG + chr7.4:30186284-30186303 MS.gene96098:intron 45.0%
CCTCTTTGCTCTTCAACAAC+AGG + chr7.4:30185393-30185412 MS.gene96098:CDS 45.0%
CCTGTTGTTGAAGAGCAAAG+AGG - chr7.4:30185396-30185415 None:intergenic 45.0%
GCATCAAGACGCTTATGAAC+TGG - chr7.4:30185307-30185326 None:intergenic 45.0%
GGGGATATTGAAGAGCTTTG+AGG - chr7.4:30187813-30187832 None:intergenic 45.0%
TAGGCAAAGCTTGGAATTCC+TGG - chr7.4:30187986-30188005 None:intergenic 45.0%
TCCCGGGTATAAAATGTTCC+TGG + chr7.4:30186399-30186418 MS.gene96098:intron 45.0%
TGGGTGATAGAGTGGGATAT+TGG - chr7.4:30187078-30187097 None:intergenic 45.0%
! CACATTTGTCAGCTCTGGTT+AGG + chr7.4:30186220-30186239 MS.gene96098:intron 45.0%
! GCTGCAGTTCATAAGGTGTT+TGG + chr7.4:30185247-30185266 MS.gene96098:CDS 45.0%
! GGTATAAGAGTATGAGAGCG+GGG + chr7.4:30185955-30185974 MS.gene96098:intron 45.0%
! TGAAAGACGGGCTTGTACAT+AGG - chr7.4:30187751-30187770 None:intergenic 45.0%
ACTGGTAACCACTAACCTGG+AGG + chr7.4:30185524-30185543 MS.gene96098:intron 50.0%
AGGGCTACTGACCCTGTTTA+TGG + chr7.4:30185358-30185377 MS.gene96098:CDS 50.0%
CTGCTATGAGGCTCTTGCTA+GGG + chr7.4:30185339-30185358 MS.gene96098:CDS 50.0%
CTGGTAACCACTAACCTGGA+GGG + chr7.4:30185525-30185544 MS.gene96098:intron 50.0%
CTTGTTACCCTCCAGGTTAG+TGG - chr7.4:30185535-30185554 None:intergenic 50.0%
GCACATAACACTACCGTGCA+AGG - chr7.4:30186282-30186301 None:intergenic 50.0%
GCACGGTAGTGTTATGTGCA+TGG + chr7.4:30186283-30186302 MS.gene96098:intron 50.0%
GTCTAGTGGGGTAAGTCTAG+TGG - chr7.4:30186714-30186733 None:intergenic 50.0%
TCTGCTATGAGGCTCTTGCT+AGG + chr7.4:30185338-30185357 MS.gene96098:CDS 50.0%
ACTAAGGCATGCCCCTTGCA+CGG + chr7.4:30186266-30186285 MS.gene96098:intron 55.0%
! GATTTGGGGTGTGGGGATGA+CGG + chr7.4:30185118-30185137 MS.gene96098:CDS 55.0%
! GGTGTGGGGATGACGGTGGT+GGG + chr7.4:30185125-30185144 MS.gene96098:CDS 65.0%
! TTGGGGTGTGGGGATGACGG+TGG + chr7.4:30185121-30185140 MS.gene96098:CDS 65.0%
!! GATGACGGTGGTGGGCCTTG+TGG + chr7.4:30185133-30185152 MS.gene96098:CDS 65.0%
! GGGTGTGGGGATGACGGTGG+TGG + chr7.4:30185124-30185143 MS.gene96098:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 30185115 30188016 30185115 ID=MS.gene96098
chr7.4 mRNA 30185115 30188016 30185115 ID=MS.gene96098.t1;Parent=MS.gene96098
chr7.4 exon 30185115 30185414 30185115 ID=MS.gene96098.t1.exon1;Parent=MS.gene96098.t1
chr7.4 CDS 30185115 30185414 30185115 ID=cds.MS.gene96098.t1;Parent=MS.gene96098.t1
chr7.4 exon 30187723 30188016 30187723 ID=MS.gene96098.t1.exon2;Parent=MS.gene96098.t1
chr7.4 CDS 30187723 30188016 30187723 ID=cds.MS.gene96098.t1;Parent=MS.gene96098.t1
Gene Sequence

>MS.gene96098

ATGGATTTGGGGTGTGGGGATGACGGTGGTGGGCCTTGTGGTGCTTGCAAGTTTTTGAGAAGAAAGTGTGTGAAAGGTTGTTGCATATTTGCACCATATTTTGAGTCATCAGGTCAAGGAAGTGCTCATTTTGCTGCAGTTCATAAGGTGTTTGGTGCTAGCAATGCTGCTAAGCTACTCACACGTATTCCAGTTCATAAGCGTCTTGATGCTGTTGTTACTCTCTGCTATGAGGCTCTTGCTAGGGCTACTGACCCTGTTTATGGTTGTGTTGCTCACCTCTTTGCTCTTCAACAACAGGTATTCATATATCAGTTTTGTTTTGATACAGTGTATAACATTTGTTTCTTCCTTAGTTTTCATTTTTCTGCCTAACTAATTCGTATATATATAACTTGGTGCTTTAAAAACTGGTAACCACTAACCTGGAGGGTAACAAGTTCATAAGACTTTATAGTAATAGATTAAAAAATATATATTAAAAATTACTTCACAAATTCAAGAAAAACTTATAATAGGTTCAAATGCAGCTAATGTATATTCTCAAGGTGTTGGAGTAATGCACTCTCCTTATTAGTTTCCTTTTAGTGGTCTCTTTTGTCCAAACAATATAAATCCTATTTATTTTCCAAAATAATATAAATCCTATTTATATATGTCATTTTTTATTAAATATTATTTTATCAATAATATTCTTTGGTAGAAGAATGGTTTAATAAATTGGTTAAATTATAATAAAGTTTATTAATTGTAAGAACTCATGCAAATTAAATAAGTATCCTAACTGGTAATTTTCTTTAGATATATAAGTATAAAATCCAGAAAAAAAGAAAAAAAAAAGGTATAAGAGTATGAGAGCGGGGAAGAAGAAAGATCATATTCATAGGGATTAGTTGCAACATCAAAGCAATAATTTTTTTGTTCCTGAACAAATTAAAGCAATATAATTTTTTTTTTTGAGCAATAAAGTTAAATGCTGTAGAATGTGTACTTTTCTAAAGTAGTGGCATGACAAAAATCACTATTCACTATTATGCATTGCACGTATGCCAAAATTTATTTTACATTGCATAGTATTGTATTGAGTATGTTCTGTTGGGTTTGACACATTTGTCAGCTCTGGTTAGGTTTTATATGGAACGAATGCATACACTAAGGCATGCCCCTTGCACGGTAGTGTTATGTGCATGGGTGTGTTTGTTTCAAGTTATAATTGTAGATTCCTGGGAATAAAATCATAAAAATGTATATACCTATGTTTCTTACAAGTTTATTAAATTTTATTCCCGGGTATAAAATGTTCCTGGAAATAGAAAAGTTTTCCCAAAGAAAATGGTGAGAATAAAGTTCCCATGGAGATGGGAATAAAATTATGTGTAATTTCCTATTTTTAAGGTTCCTTGGAATATTATAATATTAACCAAACATTTTTTTTTTTTTCTAAATACCTTGGAATAAAATTCATATCTTTATATTCCAAGGAATGTTATTCCTAAGAATAAATTTGTTACACTTGAAACAAACACACCCATGTCTTTGATCTCAAAGGCATAATATAATTAAGGACCATTAAATGCACGATCAAACACATATTTACCACTAGACTTACCCCACTAGACTTACCATGAAACTTAGTTCCTCACATTCATGTGATGATTACAGTGAAACTTAATTTTTCAAAAAAATTGATAAAAAGAAATAGTAGTCAACATTGTGTAAGTCACACAAAAAAAAAAAAAGGGAAATGTTAATAGTACCTTTAGGGCACTCTTTAAGGATTTTAGATAGTAAGTTTTTATGAAAATTTGTACATTCAAAGCATTGGAAATTGAAAATTCTTCAAGAATGCTTTTAAAACACTCATTAGCATGACCCAAATAACATTATGTAAGTCATTATGTTCATGTCAACCACACAATATTGGAATATGATGACGAGGGTTAGAACAATTTTTTTTCTTCCAATATCCCACTCTATCACCCACTCTTTAATGAAGTGAAATCAATGTGCTTTTGATACTTTAAAGATGGTTCTCACATGAAGTGATGAGATTCACATGAATTTCACATAACAACAAGAGAGTACATATTGTTCCTAACATAGTTGTATTTATACATTAATTTAAAGTTCAAGTGTCACAACATAGTGTTAGGACCCGTTTGTTACCTTATCTTTTGTGAATTAGAAAAACATAAATTATTTTAGAAGTTCAATTTATTTTTTACAAAAAAAAATTTAAAAAATCTGAAATTGCTTCGAAAAAATGTTGTTTTTAGTAGCTTCTTTTGAAAATTATTTTTAATAATTAACATTTTTTAAATAAAAAATGATTTTCTAAAAAGACAATCTCTAAAAAAATGATTTTCTAAAAAGACAATCTCTAAATTATTGGATATCAAAATAACTTTCTTATAATTACAAATGACCTTTAATTTCATGGCTAATACATTACACAACAATGGGGTAATGACAAACTCCAACATTACCCTAAACACTAATATTGATCAAGCTGTTTCATATGCCCTAACCTATTGATCATTTATATGGTATATGTGTTTTTTTAGATACCTAGTAGTCTAAATACAATTATATACTTAATTAATTACAGGTTGTGGAGTTGCAAGCAGAGTTAGCCTATGTACAAGCCCGTCTTTCAATATTGAATCGCATTTCTGAAGATCCTCAATTTCAACAGCCTCAAAGCTCTTCAATATCCCCAACTTTGCATTCATCCTCACTTCATCATTTGTCATCAAACAATGAAACTTCTTACATGTCCATGCACTTTGGTCATCCTCTTCAACTTCAATATCATCCAACACCTTTTGAAAATCCATCATACTATTCCTATTATCAACAACTTCATGACCAGGAATTCCAAGCTTTGCCTAGAGAATTTTAG
Protein sequence

>MS.gene96098.t1

MDLGCGDDGGGPCGACKFLRRKCVKGCCIFAPYFESSGQGSAHFAAVHKVFGASNAAKLLTRIPVHKRLDAVVTLCYEALARATDPVYGCVAHLFALQQQVVELQAELAYVQARLSILNRISEDPQFQQPQSSSISPTLHSSSLHHLSSNNETSYMSMHFGHPLQLQYHPTPFENPSYYSYYQQLHDQEFQALPREF