AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96249

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96249.t1 MTR_2g019190 92.537 134 5 3 1 130 1 133 7.41e-82 236
MS.gene96249.t1 MTR_8g107300 72.180 133 16 6 1 129 1 116 6.99e-55 167
MS.gene96249.t1 MTR_8g107300 78.261 92 9 2 38 129 1 81 2.63e-43 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96249.t1 AT1G30135 44.275 131 67 4 1 130 3 128 7.68e-27 97.4
MS.gene96249.t1 AT2G34600 41.481 135 67 7 1 131 20 146 2.55e-17 73.6

Find 26 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTTACATGGGGTGATTT+TGG 0.252416 2.2:-65448802 None:intergenic
TGCACTATTGTTGGTGTTGA+TGG 0.316876 2.2:-65452847 None:intergenic
GATGATATGGAGATGCTTCT+TGG 0.331037 2.2:-65452957 None:intergenic
TACTGATGTCACAGAGCTTC+AGG 0.335972 2.2:+65449022 MS.gene96249:CDS
TGCCAGCATTAAGATGGATT+TGG 0.344981 2.2:-65452776 None:intergenic
AATTGTTGTATGGAGGAAAA+AGG 0.349461 2.2:-65448769 None:intergenic
CATCTTGCCATCGTAGAAAA+TGG 0.392349 2.2:-65448994 None:intergenic
GTTGATGGTTCTGACCCTAT+TGG 0.411337 2.2:-65452832 None:intergenic
TCTATTTGCCAGCATTAAGA+TGG 0.436860 2.2:-65452782 None:intergenic
TTCATGGAAAGACCTGGTCC+AGG 0.470860 2.2:-65452892 None:intergenic
TCTACAAAAGAGAAAGAATA+GGG 0.475189 2.2:+65452933 MS.gene96249:CDS
TTCTACAAAAGAGAAAGAAT+AGG 0.501770 2.2:+65452932 MS.gene96249:CDS
TGAGGAGATTGCACTATTGT+TGG 0.504260 2.2:-65452856 None:intergenic
TGATTGCTAGAATTGTTGTA+TGG 0.511394 2.2:-65448779 None:intergenic
GGCCAAATCCATCTTAATGC+TGG 0.519970 2.2:+65452774 MS.gene96249:intron
CCATGAAAAGATCTTTGCAA+AGG 0.529406 2.2:+65452908 MS.gene96249:CDS
CCTCATCAATTATATAGCCC+TGG 0.530434 2.2:+65452874 MS.gene96249:CDS
TCAATAAAGGGTAGAAGAAG+AGG 0.532802 2.2:+65448926 MS.gene96249:intron
GATGAGGAGGAATTGCAACT+TGG 0.553645 2.2:+65448739 None:intergenic
CAATTATATAGCCCTGGACC+AGG 0.570726 2.2:+65452880 MS.gene96249:CDS
AATGCTGGCAAATAGAATGA+AGG 0.571363 2.2:+65452789 MS.gene96249:CDS
GAAATACAGAAAAGATGAGG+AGG 0.572260 2.2:+65448726 None:intergenic
TCATGGAAAGACCTGGTCCA+GGG 0.597585 2.2:-65452891 None:intergenic
TTGCTAGAATTGTTGTATGG+AGG 0.628547 2.2:-65448776 None:intergenic
CCAGGGCTATATAATTGATG+AGG 0.642818 2.2:-65452874 None:intergenic
CCATCGTAGAAAATGGTCAG+TGG 0.671131 2.2:-65448987 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTATAATATTTATAA+AGG + chr2.2:65452335-65452354 MS.gene96249:intron 0.0%
!! AGTATAAAATAGTATAAATT+TGG - chr2.2:65450603-65450622 None:intergenic 10.0%
!! TAAATTTATATCAAGTATTT+AGG - chr2.2:65450180-65450199 None:intergenic 10.0%
!! TTTATAATAAAAAGCTTAAT+AGG - chr2.2:65451356-65451375 None:intergenic 10.0%
!!! ATAAATAAATATAGATTTTG+AGG + chr2.2:65450218-65450237 MS.gene96249:intron 10.0%
!!! TATTTTGAAATAATTTGAAA+TGG - chr2.2:65449671-65449690 None:intergenic 10.0%
!!! TTATTTTAACATTGATTTAA+CGG + chr2.2:65449440-65449459 MS.gene96249:intron 10.0%
!!! TTTTTTTTACAAATTTGTTA+CGG - chr2.2:65452233-65452252 None:intergenic 10.0%
!! AAAAAATTAACAAATTCATC+CGG - chr2.2:65450536-65450555 None:intergenic 15.0%
!! AAAAATACAAATTCATATCA+AGG - chr2.2:65450510-65450529 None:intergenic 15.0%
!! AAAATACAAATTCATATCAA+GGG - chr2.2:65450509-65450528 None:intergenic 15.0%
!! AAACCTTAAAAAGAAAATTA+AGG + chr2.2:65450774-65450793 MS.gene96249:intron 15.0%
!! AATAAAATGATAAGAAAAGA+TGG + chr2.2:65452374-65452393 MS.gene96249:intron 15.0%
!! AATTTAACCATTGAAAAAAT+TGG + chr2.2:65452444-65452463 MS.gene96249:intron 15.0%
!! ATAAAATGATAAGAAAAGAT+GGG + chr2.2:65452375-65452394 MS.gene96249:intron 15.0%
!! TAAAATGTTACAAATTGTTA+CGG - chr2.2:65452318-65452337 None:intergenic 15.0%
!!! AAATATTTTAGAAAGAGTAA+AGG - chr2.2:65451081-65451100 None:intergenic 15.0%
!!! ATATAAATTTAGTTTTCATG+AGG + chr2.2:65450189-65450208 MS.gene96249:intron 15.0%
!!! ATGAATTTGTATTTTTTTAC+CGG + chr2.2:65450514-65450533 MS.gene96249:intron 15.0%
!!! GTAAATGACTTTTTTTTTTT+TGG - chr2.2:65449764-65449783 None:intergenic 15.0%
!!! GTTTTTTTTTTAAAGAGAAA+AGG - chr2.2:65452154-65452173 None:intergenic 15.0%
!!! TATCCTTAATTTTCTTTTTA+AGG - chr2.2:65450780-65450799 None:intergenic 15.0%
!!! TCATTTTTGTTAAAAAAGTT+TGG + chr2.2:65450926-65450945 MS.gene96249:intron 15.0%
!!! TTGTTAATTTGTTCAATAAA+GGG + chr2.2:65448914-65448933 MS.gene96249:intron 15.0%
!!! TTTGTTAATTTGTTCAATAA+AGG + chr2.2:65448913-65448932 MS.gene96249:intron 15.0%
!! AACTTCAGAAATATCAATAA+TGG - chr2.2:65451386-65451405 None:intergenic 20.0%
!! AATCCAATGACTATAAATAT+TGG - chr2.2:65451012-65451031 None:intergenic 20.0%
!! AATTAACTGCTAGAAATATT+AGG - chr2.2:65450664-65450683 None:intergenic 20.0%
!! ATACCAACAACAAAAAATTA+CGG + chr2.2:65452261-65452280 MS.gene96249:intron 20.0%
!! CTAAATAATCAATCATATGA+AGG - chr2.2:65451430-65451449 None:intergenic 20.0%
!! CTATAAAGTTTGAAAATTCT+TGG - chr2.2:65452597-65452616 None:intergenic 20.0%
!! GGTTATATCATATTCATTTA+TGG + chr2.2:65449221-65449240 MS.gene96249:intron 20.0%
!! TAAATGATGAATTCTTATGA+AGG - chr2.2:65451478-65451497 None:intergenic 20.0%
!! TTATTTAGTAGCTGATATAT+TGG + chr2.2:65451442-65451461 MS.gene96249:intron 20.0%
!!! AAAACAAATTTTGCTTACAT+GGG - chr2.2:65448815-65448834 None:intergenic 20.0%
!!! AATGACTTTTTTTTTTTTGG+TGG - chr2.2:65449761-65449780 None:intergenic 20.0%
!!! ACATGATTTTTATCACAAAT+GGG - chr2.2:65452131-65452150 None:intergenic 20.0%
!!! CATAAAAAGTCACAATTTTA+AGG - chr2.2:65449511-65449530 None:intergenic 20.0%
!!! CATAAAATAGGCTTTTAAAT+AGG + chr2.2:65451581-65451600 MS.gene96249:intron 20.0%
!!! CTAAACATTGATTGAAAATA+GGG - chr2.2:65451756-65451775 None:intergenic 20.0%
!!! CTATTATTATTCTTTTGGAA+AGG + chr2.2:65451943-65451962 MS.gene96249:intron 20.0%
!!! TAAACATTGATTGAAAATAG+GGG - chr2.2:65451755-65451774 None:intergenic 20.0%
!!! TAATTGTTTTGGTTTTAGTT+TGG + chr2.2:65449273-65449292 MS.gene96249:intron 20.0%
!!! TACTGCTATTATTATTCTTT+TGG + chr2.2:65451938-65451957 MS.gene96249:intron 20.0%
!!! TCTAAACATTGATTGAAAAT+AGG - chr2.2:65451757-65451776 None:intergenic 20.0%
!!! TGCGGAAAATAATTTTTAAA+AGG + chr2.2:65450296-65450315 MS.gene96249:intron 20.0%
!!! TTTTGATGGTTAGTTTAAAT+TGG - chr2.2:65450116-65450135 None:intergenic 20.0%
! AAATAGGCCAAAACTTATTA+AGG - chr2.2:65451528-65451547 None:intergenic 25.0%
! AAATGAACCCATGAAAATAT+TGG - chr2.2:65452481-65452500 None:intergenic 25.0%
! AAATGGATATTATTGCTGAA+AGG - chr2.2:65449654-65449673 None:intergenic 25.0%
! ACCAAAACAATTAATTAAGC+TGG - chr2.2:65449266-65449285 None:intergenic 25.0%
! AGGAAATAATTAGATGTATG+AGG - chr2.2:65451061-65451080 None:intergenic 25.0%
! ATAGACCATGATAAGATTTA+GGG + chr2.2:65452413-65452432 MS.gene96249:intron 25.0%
! ATCATATCATCTTCAATCAT+AGG - chr2.2:65449098-65449117 None:intergenic 25.0%
! CAGTATAAGGTCAATTATTT+CGG + chr2.2:65452679-65452698 MS.gene96249:intron 25.0%
! CCAAAACAATTAATTAAGCT+GGG - chr2.2:65449265-65449284 None:intergenic 25.0%
! CTATAAATATTGGTCCATTA+TGG - chr2.2:65451002-65451021 None:intergenic 25.0%
! CTATGAGACTCATAATATAT+GGG - chr2.2:65449795-65449814 None:intergenic 25.0%
! TAATAAAAAGCTTAATAGGC+CGG - chr2.2:65451352-65451371 None:intergenic 25.0%
! TATAGACCATGATAAGATTT+AGG + chr2.2:65452412-65452431 MS.gene96249:intron 25.0%
! TCAATTATTTCGGATAAGAA+AGG + chr2.2:65452689-65452708 MS.gene96249:intron 25.0%
! TCTACAAAAGAGAAAGAATA+GGG + chr2.2:65452933-65452952 MS.gene96249:CDS 25.0%
! TCTATGAGACTCATAATATA+TGG - chr2.2:65449796-65449815 None:intergenic 25.0%
! TGACCTACTTCAAAAATTAT+AGG - chr2.2:65451689-65451708 None:intergenic 25.0%
! TTCATTTATGGATCATAAGA+AGG + chr2.2:65449233-65449252 MS.gene96249:intron 25.0%
! TTCTACAAAAGAGAAAGAAT+AGG + chr2.2:65452932-65452951 MS.gene96249:CDS 25.0%
!! AAACAAATTTTGCTTACATG+GGG - chr2.2:65448814-65448833 None:intergenic 25.0%
!! AAAGTGATAATGTTTGCATT+TGG - chr2.2:65449339-65449358 None:intergenic 25.0%
!! GAAAACAAATTTTGCTTACA+TGG - chr2.2:65448816-65448835 None:intergenic 25.0%
!! GACATGATTTTTATCACAAA+TGG - chr2.2:65452132-65452151 None:intergenic 25.0%
!! TAAAAGCCTATTTTATGCTA+AGG - chr2.2:65451578-65451597 None:intergenic 25.0%
!! TTTCATGGGTTCATTTTATA+AGG + chr2.2:65452485-65452504 MS.gene96249:intron 25.0%
!!! AAATTTGGTGTGAATTTCAA+TGG - chr2.2:65450588-65450607 None:intergenic 25.0%
!!! AAATTTGTTACGGTAATTGT+TGG - chr2.2:65452223-65452242 None:intergenic 25.0%
!!! ACTGGTCTTATTTAGAAATT+TGG - chr2.2:65449962-65449981 None:intergenic 25.0%
!!! ATGCCTATAATTTTTGAAGT+AGG + chr2.2:65451683-65451702 MS.gene96249:intron 25.0%
!!! ATGTGATTGTTGTTTTCTTT+GGG + chr2.2:65448875-65448894 MS.gene96249:intron 25.0%
!!! CAAACATATTTTTGTGCTTT+AGG + chr2.2:65451502-65451521 MS.gene96249:intron 25.0%
!!! CATTGATTTAACGGATTTTT+CGG + chr2.2:65449449-65449468 MS.gene96249:intron 25.0%
!!! CTTTTTTTTTTTTGGTGGAA+AGG - chr2.2:65449756-65449775 None:intergenic 25.0%
!!! TATGTGATTGTTGTTTTCTT+TGG + chr2.2:65448874-65448893 MS.gene96249:intron 25.0%
!!! TTACCGTAATTTTTTGTTGT+TGG - chr2.2:65452267-65452286 None:intergenic 25.0%
!!! TTAGTTTAAATTGGTTTGAG+AGG - chr2.2:65450107-65450126 None:intergenic 25.0%
AAAAATTATAGGCATGAGTC+TGG - chr2.2:65451678-65451697 None:intergenic 30.0%
AAATACACATATTGAGAGAG+AGG + chr2.2:65449891-65449910 MS.gene96249:intron 30.0%
AAATTACAGAAAGAGAGAGA+AGG - chr2.2:65451109-65451128 None:intergenic 30.0%
AATTGTTGTATGGAGGAAAA+AGG - chr2.2:65448772-65448791 None:intergenic 30.0%
ACACAAACAACGTCATATTT+AGG + chr2.2:65449994-65450013 MS.gene96249:intron 30.0%
ACTCATAATATATGGGCAAA+AGG - chr2.2:65449788-65449807 None:intergenic 30.0%
ATGAAAATATTGGTCCATGA+TGG - chr2.2:65452471-65452490 None:intergenic 30.0%
ATGTAAGCCTACTTCTAAAT+AGG - chr2.2:65451544-65451563 None:intergenic 30.0%
ATTGCTGAAAGGTTAAATCA+AGG - chr2.2:65449643-65449662 None:intergenic 30.0%
CACCACTAATTGAAAACTAT+GGG - chr2.2:65451165-65451184 None:intergenic 30.0%
CCTATTTAATTAAACAGACC+AGG + chr2.2:65451290-65451309 MS.gene96249:intron 30.0%
CCTGGTCTGTTTAATTAAAT+AGG - chr2.2:65451293-65451312 None:intergenic 30.0%
CTTGTTAATCAGATTATCTG+CGG + chr2.2:65450278-65450297 MS.gene96249:intron 30.0%
GGACCAATATTTATAGTCAT+TGG + chr2.2:65451006-65451025 MS.gene96249:intron 30.0%
TACCCATTGAACTATATTCA+CGG + chr2.2:65450436-65450455 MS.gene96249:intron 30.0%
TAGACCATGATAAGATTTAG+GGG + chr2.2:65452414-65452433 MS.gene96249:intron 30.0%
TGAATATAGTTCAATGGGTA+GGG - chr2.2:65450436-65450455 None:intergenic 30.0%
TGATTGCTAGAATTGTTGTA+TGG - chr2.2:65448782-65448801 None:intergenic 30.0%
TTCATGTGTTATGCAGTATA+AGG + chr2.2:65452666-65452685 MS.gene96249:intron 30.0%
TTGAAAAAATTGGTCCATCA+TGG + chr2.2:65452454-65452473 MS.gene96249:intron 30.0%
TTGCTGAAAGGTTAAATCAA+GGG - chr2.2:65449642-65449661 None:intergenic 30.0%
TTTATCACAAATGGGAGAAA+AGG - chr2.2:65452123-65452142 None:intergenic 30.0%
! AACCCATAGTTTTCAATTAG+TGG + chr2.2:65451160-65451179 MS.gene96249:intron 30.0%
! ATCATGGACCAATATTTTCA+TGG + chr2.2:65452470-65452489 MS.gene96249:intron 30.0%
! CCCAGCTTAATTAATTGTTT+TGG + chr2.2:65449262-65449281 MS.gene96249:intron 30.0%
! CTTTAGGCCTTAATAAGTTT+TGG + chr2.2:65451518-65451537 MS.gene96249:intron 30.0%
! TCAGACTCAAGCTTTTTATA+AGG + chr2.2:65451267-65451286 MS.gene96249:intron 30.0%
! TCATGGACCAATATTTTCAT+GGG + chr2.2:65452471-65452490 MS.gene96249:intron 30.0%
! TTACCTGAAAAGTATAGTTC+AGG - chr2.2:65452027-65452046 None:intergenic 30.0%
! TTTCAATTAGTGGTGGAAAT+AGG + chr2.2:65451170-65451189 MS.gene96249:intron 30.0%
!! ATGTTAGCATTTGATTTGAG+TGG + chr2.2:65449072-65449091 MS.gene96249:intron 30.0%
!! TGACGTTGTTTGTGTTATTT+CGG - chr2.2:65449989-65450008 None:intergenic 30.0%
!! TTTGACTTTGATTTTGGTGT+TGG - chr2.2:65450145-65450164 None:intergenic 30.0%
!!! TGATGGACCAATTTTTTCAA+TGG - chr2.2:65452454-65452473 None:intergenic 30.0%
!!! TTTGATTTTGGTGTTGGTTT+TGG - chr2.2:65450139-65450158 None:intergenic 30.0%
AAAAAGTGACGACACCAATA+GGG + chr2.2:65452818-65452837 MS.gene96249:CDS 35.0%
AATGCTGGCAAATAGAATGA+AGG + chr2.2:65452789-65452808 MS.gene96249:CDS 35.0%
ACAGAATGAGTAACTCAAAC+TGG + chr2.2:65449200-65449219 MS.gene96249:intron 35.0%
ACCTTGTTAATAAACGAGCT+GGG - chr2.2:65451248-65451267 None:intergenic 35.0%
ATCAAGTATTTAGGCTGTTG+TGG - chr2.2:65450171-65450190 None:intergenic 35.0%
CAAACAACGTCATATTTAGG+CGG + chr2.2:65449997-65450016 MS.gene96249:intron 35.0%
CAAAGTCGAGAAATATTTCC+AGG - chr2.2:65452513-65452532 None:intergenic 35.0%
CCACCACTAATTGAAAACTA+TGG - chr2.2:65451166-65451185 None:intergenic 35.0%
CCATGAAAAGATCTTTGCAA+AGG + chr2.2:65452908-65452927 MS.gene96249:CDS 35.0%
CTCCGTGAATATAGTTCAAT+GGG - chr2.2:65450441-65450460 None:intergenic 35.0%
CTGTCGCATACATATTATCA+TGG - chr2.2:65449124-65449143 None:intergenic 35.0%
GTCAGACCTTAGCATAAAAT+AGG + chr2.2:65451569-65451588 MS.gene96249:intron 35.0%
GTGAATATAGTTCAATGGGT+AGG - chr2.2:65450437-65450456 None:intergenic 35.0%
TAATTAAACAGACCAGGCTA+AGG + chr2.2:65451296-65451315 MS.gene96249:intron 35.0%
TCAAGTATTTAGGCTGTTGT+GGG - chr2.2:65450170-65450189 None:intergenic 35.0%
TCAATAAAGGGTAGAAGAAG+AGG + chr2.2:65448926-65448945 MS.gene96249:intron 35.0%
TCTATTTGCCAGCATTAAGA+TGG - chr2.2:65452785-65452804 None:intergenic 35.0%
TCTCCGTGAATATAGTTCAA+TGG - chr2.2:65450442-65450461 None:intergenic 35.0%
TGAACCCCTAAATCTTATCA+TGG - chr2.2:65452421-65452440 None:intergenic 35.0%
TTAAAAAAGTTTGGCGTGAG+TGG + chr2.2:65450935-65450954 MS.gene96249:intron 35.0%
TTGAAAATAGGGGTGGAAAT+AGG - chr2.2:65451745-65451764 None:intergenic 35.0%
TTGCTAGAATTGTTGTATGG+AGG - chr2.2:65448779-65448798 None:intergenic 35.0%
! ACATTGATTGAAAATAGGGG+TGG - chr2.2:65451752-65451771 None:intergenic 35.0%
! AGCCTATTTAAAGCCTAACT+CGG + chr2.2:65451222-65451241 MS.gene96249:intron 35.0%
! AGGCTTTTAAATAGGCTTTC+AGG + chr2.2:65451589-65451608 MS.gene96249:intron 35.0%
! AGTTTGAGTTACTCATTCTG+TGG - chr2.2:65449201-65449220 None:intergenic 35.0%
! CCATAGTTTTCAATTAGTGG+TGG + chr2.2:65451163-65451182 MS.gene96249:intron 35.0%
! CCTTTGCAAAGATCTTTTCA+TGG - chr2.2:65452911-65452930 None:intergenic 35.0%
! GGTTCATTTTATAAGGAACC+TGG + chr2.2:65452492-65452511 MS.gene96249:intron 35.0%
! GTACCTGAACTATACTTTTC+AGG + chr2.2:65452021-65452040 MS.gene96249:intron 35.0%
!! AGTTAGGCTTTAAATAGGCT+AGG - chr2.2:65451222-65451241 None:intergenic 35.0%
!! GTGTAAGTATTGGTCCATAA+TGG + chr2.2:65450985-65451004 MS.gene96249:intron 35.0%
!! GTTTTGGCCTATTTAGAAGT+AGG + chr2.2:65451534-65451553 MS.gene96249:intron 35.0%
!! TTGTGTTATTTCGGTCTAAC+TGG - chr2.2:65449980-65449999 None:intergenic 35.0%
!!! TTTTGAAGTAGGTCATGCTT+AGG + chr2.2:65451694-65451713 MS.gene96249:intron 35.0%
AAAAGCCTATTAAGCCCATC+AGG + chr2.2:65451326-65451345 MS.gene96249:intron 40.0%
AAGTATTTAGGCTGTTGTGG+GGG - chr2.2:65450168-65450187 None:intergenic 40.0%
ACCAATACTTACACAAGTCC+AGG - chr2.2:65450979-65450998 None:intergenic 40.0%
ACCACTTACTATCTCTTTCG+TGG - chr2.2:65450807-65450826 None:intergenic 40.0%
ACCTCGAAAATAAGCCTATG+AGG + chr2.2:65451643-65451662 MS.gene96249:intron 40.0%
AGGTTTCACTACCAACTTGT+CGG - chr2.2:65450760-65450779 None:intergenic 40.0%
AGTAAGTGGTAGGTTCCTAT+AGG + chr2.2:65450817-65450836 MS.gene96249:intron 40.0%
ATAAGCCTATGAGGGGTAAT+AGG + chr2.2:65451652-65451671 MS.gene96249:intron 40.0%
CAAGAGTCGAGTTTGAATCT+CGG - chr2.2:65450400-65450419 None:intergenic 40.0%
CAAGTATTTAGGCTGTTGTG+GGG - chr2.2:65450169-65450188 None:intergenic 40.0%
CATCTTGCCATCGTAGAAAA+TGG - chr2.2:65448997-65449016 None:intergenic 40.0%
CCAGGGCTATATAATTGATG+AGG - chr2.2:65452877-65452896 None:intergenic 40.0%
CCTCATCAATTATATAGCCC+TGG + chr2.2:65452874-65452893 MS.gene96249:CDS 40.0%
CCTCGAAAATAAGCCTATGA+GGG + chr2.2:65451644-65451663 MS.gene96249:intron 40.0%
CGAAAGAGATAGTAAGTGGT+AGG + chr2.2:65450807-65450826 MS.gene96249:intron 40.0%
CTCGAAAATAAGCCTATGAG+GGG + chr2.2:65451645-65451664 MS.gene96249:intron 40.0%
CTTAGCTTAAGTGCTATGCA+CGG - chr2.2:65450699-65450718 None:intergenic 40.0%
CTTTAAATAGGCTAGGTCGT+AGG - chr2.2:65451215-65451234 None:intergenic 40.0%
GAAAAAGTGACGACACCAAT+AGG + chr2.2:65452817-65452836 MS.gene96249:CDS 40.0%
GAACTATATTCACGGAGAGT+CGG + chr2.2:65450444-65450463 MS.gene96249:intron 40.0%
GACCTTGTTAATAAACGAGC+TGG - chr2.2:65451249-65451268 None:intergenic 40.0%
GATGATATGGAGATGCTTCT+TGG - chr2.2:65452960-65452979 None:intergenic 40.0%
TCCACGAAAGAGATAGTAAG+TGG + chr2.2:65450803-65450822 MS.gene96249:intron 40.0%
TCGAGTTTGAATCTCGGATT+CGG - chr2.2:65450394-65450413 None:intergenic 40.0%
TGAGGAGATTGCACTATTGT+TGG - chr2.2:65452859-65452878 None:intergenic 40.0%
TGAGTTACTCATTCTGTGGT+AGG - chr2.2:65449197-65449216 None:intergenic 40.0%
TTACACAAGTCCAGGCTAAA+TGG - chr2.2:65450971-65450990 None:intergenic 40.0%
! ATTAGTGGTGGAAATAGGTC+AGG + chr2.2:65451175-65451194 MS.gene96249:intron 40.0%
! GATCTTTTCATGGAAAGACC+TGG - chr2.2:65452901-65452920 None:intergenic 40.0%
! GCTTCATCAGCCCTTTTAAA+AGG + chr2.2:65451611-65451630 MS.gene96249:intron 40.0%
! TGCCAGCATTAAGATGGATT+TGG - chr2.2:65452779-65452798 None:intergenic 40.0%
! TTGCTTACATGGGGTGATTT+TGG - chr2.2:65448805-65448824 None:intergenic 40.0%
!! AAATATGGTGCCATTTAGCC+TGG + chr2.2:65450958-65450977 MS.gene96249:intron 40.0%
!! ATAGGCTTATTTTCGAGGTC+TGG - chr2.2:65451642-65451661 None:intergenic 40.0%
!! ATCAGCCCTTTTAAAAGGTC+AGG + chr2.2:65451616-65451635 MS.gene96249:intron 40.0%
!! GAGATTCCTTAAGAGTGTTC+CGG + chr2.2:65449599-65449618 MS.gene96249:intron 40.0%
!! TAAGGTCTTAAAGAGTGCTC+CGG - chr2.2:65449493-65449512 None:intergenic 40.0%
!! TCTGGCCTGACCTTTTAAAA+GGG - chr2.2:65451624-65451643 None:intergenic 40.0%
!! TGCACTATTGTTGGTGTTGA+TGG - chr2.2:65452850-65452869 None:intergenic 40.0%
!! TGGGGGTTTGACTTTGATTT+TGG - chr2.2:65450151-65450170 None:intergenic 40.0%
!!! GGTGTTGGTTTTGGTTTTGA+TGG - chr2.2:65450130-65450149 None:intergenic 40.0%
AAGTGGTAGGTTCCTATAGG+TGG + chr2.2:65450820-65450839 MS.gene96249:intron 45.0%
AATAGTCGTTGCCGACAAGT+TGG + chr2.2:65450746-65450765 MS.gene96249:intron 45.0%
ACAACAACGCCACTCAAATG+AGG + chr2.2:65450077-65450096 MS.gene96249:intron 45.0%
AGAGGTAGTCCTCATTTGAG+TGG - chr2.2:65450089-65450108 None:intergenic 45.0%
AGTGTTCCGGAACACTCTTA+AGG - chr2.2:65449608-65449627 None:intergenic 45.0%
CAATTATATAGCCCTGGACC+AGG + chr2.2:65452880-65452899 MS.gene96249:CDS 45.0%
CATGCTCATACACCACCTAT+AGG - chr2.2:65450835-65450854 None:intergenic 45.0%
CATTTGAGTGGCGTTGTTGT+TGG - chr2.2:65450077-65450096 None:intergenic 45.0%
CCATCGTAGAAAATGGTCAG+TGG - chr2.2:65448990-65449009 None:intergenic 45.0%
CGGATTCGGATTCACTTAAG+TGG - chr2.2:65450380-65450399 None:intergenic 45.0%
GCCCAGCTCGTTTATTAACA+AGG + chr2.2:65451244-65451263 MS.gene96249:intron 45.0%
GCCTGGACTTGTGTAAGTAT+TGG + chr2.2:65450975-65450994 MS.gene96249:intron 45.0%
GGCCAAATCCATCTTAATGC+TGG + chr2.2:65452774-65452793 MS.gene96249:intron 45.0%
GTTTGGCGTGAGTGGAAATA+TGG + chr2.2:65450943-65450962 MS.gene96249:intron 45.0%
TACTGATGTCACAGAGCTTC+AGG + chr2.2:65449022-65449041 MS.gene96249:CDS 45.0%
TGTTAACGTGAATGTCGTGC+AGG + chr2.2:65452753-65452772 MS.gene96249:intron 45.0%
! CCACTGACCATTTTCTACGA+TGG + chr2.2:65448987-65449006 MS.gene96249:CDS 45.0%
! GTCTGGCCTGACCTTTTAAA+AGG - chr2.2:65451625-65451644 None:intergenic 45.0%
!! AAGGTCTTAAAGAGTGCTCC+GGG - chr2.2:65449492-65449511 None:intergenic 45.0%
!! CCCTCATAGGCTTATTTTCG+AGG - chr2.2:65451647-65451666 None:intergenic 45.0%
!! GGCCGAGTTAGGCTTTAAAT+AGG - chr2.2:65451227-65451246 None:intergenic 45.0%
!! GGTCTTGTTAACGAGTGTTC+CGG - chr2.2:65449621-65449640 None:intergenic 45.0%
!! GTTGATGGTTCTGACCCTAT+TGG - chr2.2:65452835-65452854 None:intergenic 45.0%
!! TGGCGTTGTTGTTGGTTTGT+TGG - chr2.2:65450069-65450088 None:intergenic 45.0%
!!! CTTTTTTGCAAGCCTTAGCC+TGG - chr2.2:65451311-65451330 None:intergenic 45.0%
!!! GGCTGTTGCTGATTTTGCAT+TGG - chr2.2:65448969-65448988 None:intergenic 45.0%
!! AAATTAAATTTAAAAATGAA+AGG - chr2.2:65448852-65448871 None:intergenic 5.0%
!! AATAAATAAATAATTGATAA+CGG + chr2.2:65451999-65452018 MS.gene96249:intron 5.0%
!!! ATATCTTTTATTAAAAAAAA+AGG - chr2.2:65451885-65451904 None:intergenic 5.0%
!!! ATTTTTTTTTTAATAAGAAA+AGG - chr2.2:65452092-65452111 None:intergenic 5.0%
GAAATGTTAACGAGTGCCCC+CGG + chr2.2:65449471-65449490 MS.gene96249:intron 50.0%
TCATGGAAAGACCTGGTCCA+GGG - chr2.2:65452894-65452913 None:intergenic 50.0%
TCTGGCCTATTACCCCTCAT+AGG - chr2.2:65451660-65451679 None:intergenic 50.0%
TGCTTAGGCCTTACAAAGCC+CGG + chr2.2:65451709-65451728 MS.gene96249:intron 50.0%
TTCATGGAAAGACCTGGTCC+AGG - chr2.2:65452895-65452914 None:intergenic 50.0%
!! AGGTCTTAAAGAGTGCTCCG+GGG - chr2.2:65449491-65449510 None:intergenic 50.0%
AATAGGTCAGGCCGTTCGAC+AGG + chr2.2:65451187-65451206 MS.gene96249:intron 55.0%
AGCTTAATAGGCCGGCCTGA+TGG - chr2.2:65451344-65451363 None:intergenic 55.0%
ATAGGTCAGGCCGTTCGACA+GGG + chr2.2:65451188-65451207 MS.gene96249:intron 55.0%
GCCTATTAAGCCCATCAGGC+CGG + chr2.2:65451330-65451349 MS.gene96249:intron 55.0%
GCTTAATAGGCCGGCCTGAT+GGG - chr2.2:65451343-65451362 None:intergenic 55.0%
TAAACGAGCTGGGCCGAGTT+AGG - chr2.2:65451238-65451257 None:intergenic 55.0%
!! GGTCTTAAAGAGTGCTCCGG+GGG - chr2.2:65449490-65449509 None:intergenic 55.0%
GGGGTGGAAATAGGCTACGC+CGG - chr2.2:65451736-65451755 None:intergenic 60.0%
GGGTGGAAATAGGCTACGCC+GGG - chr2.2:65451735-65451754 None:intergenic 60.0%
TAGGTCAGGCCGTTCGACAG+GGG + chr2.2:65451189-65451208 MS.gene96249:intron 60.0%
!! GCCGGCCTGATGGGCTTAAT+AGG - chr2.2:65451334-65451353 None:intergenic 60.0%
AGGCCTTACAAAGCCCGGCC+CGG + chr2.2:65451714-65451733 MS.gene96249:intron 65.0%
GAAATAGGCTACGCCGGGCC+GGG - chr2.2:65451730-65451749 None:intergenic 65.0%
GGAAATAGGCTACGCCGGGC+CGG - chr2.2:65451731-65451750 None:intergenic 65.0%
GGTCGTAGGCCCCTGTCGAA+CGG - chr2.2:65451201-65451220 None:intergenic 65.0%
! ACGCCGGGCCGGGCTTTGTA+AGG - chr2.2:65451720-65451739 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 65448740 65452984 65448740 ID=MS.gene96249
chr2.2 mRNA 65448740 65452984 65448740 ID=MS.gene96249.t1;Parent=MS.gene96249
chr2.2 exon 65448740 65448816 65448740 ID=MS.gene96249.t1.exon1;Parent=MS.gene96249.t1
chr2.2 CDS 65448740 65448816 65448740 ID=cds.MS.gene96249.t1;Parent=MS.gene96249.t1
chr2.2 exon 65448935 65449043 65448935 ID=MS.gene96249.t1.exon2;Parent=MS.gene96249.t1
chr2.2 CDS 65448935 65449043 65448935 ID=cds.MS.gene96249.t1;Parent=MS.gene96249.t1
chr2.2 exon 65452775 65452984 65452775 ID=MS.gene96249.t1.exon3;Parent=MS.gene96249.t1
chr2.2 CDS 65452775 65452984 65452775 ID=cds.MS.gene96249.t1;Parent=MS.gene96249.t1
Gene Sequence

>MS.gene96249

ATGAGGAGGAATTGCAACTTGGAACTATGCCTTTTTCCTCCATACAACAATTCTAGCAATCACCAAAATCACCCCATGTAAGCAAAATTTGTTTTCTTTGATTGTACTTCCTTTCATTTTTAAATTTAATTTGTTATGTGATTGTTGTTTTCTTTGGGTATTGAATTAGTTTTTTTGTTAATTTGTTCAATAAAGGGTAGAAGAAGAGGATAGTAATGAGAGCACTCCAATGCAAAATCAGCAACAGCCACTGACCATTTTCTACGATGGCAAGATGTGTGTTACTGATGTCACAGAGCTTCAGGTAAATAGATCTTTATATATATTATTTTATGTTAGCATTTGATTTGAGTGGCCTATGATTGAAGATGATATGATTCTCCATGATAATATGTATGCGACAGTATCATTTTCATCTTTTTAGTTATTATATATGTGTCTGATTTGATTCATACCTACCACAGAATGAGTAACTCAAACTGGTTATATCATATTCATTTATGGATCATAAGAAGGTCTAATCCCAGCTTAATTAATTGTTTTGGTTTTAGTTTGGCAACGAGTCAGAGAATTAGTTTGAGTATTTTTTTTCTTCTCCAAATGCAAACATTATCACTTTGATCTTCACAAATTTTCGTTCTAATTTCTTTCAATAATAACGAATATATAGTATCTATTATATTATTTTTGTGTCTCAATTTTATTTTAACATTGATTTAACGGATTTTTCGGAAATGTTAACGAGTGCCCCCGGAGCACTCTTTAAGACCTTAAAATTGTGACTTTTTATGAAAAGATGTGTAATCAACGCATTAGAAATTGAAATATTTGACATTTTTATAAAATAATTTATTCTTTTGAGATTCCTTAAGAGTGTTCCGGAACACTCGTTAACAAGACCCTTGATTTAACCTTTCAGCAATAATATCCATTTCAAATTATTTCAAAATAATATCCATTTCAAATTATTTCAAAATAAGTAGTAACATTTAGTGATATTTTAGTAATTTACTCCTTTCCACCAAAAAAAAAAAAGTCATTTACTCCTTTTGCCCATATATTATGAGTCTCATAGAATAATATTTTTATCAAATTGAATTGATCTATCAATCACTATTATAATTGCAAAGTGAATGATAATGACTTCTTTAAAATACACATATTGAGAGAGAGGAGTATACATGTATATATTTTTTTAAACGTCAAAATTTATTAATGACCAAATTTCTAAATAAGACCAGTTAGACCGAAATAACACAAACAACGTCATATTTAGGCGGAATATCGAAAAGAAAATTTATACATCTCATACATCAAATACAAAATCCAACAAACCAACAACAACGCCACTCAAATGAGGACTACCTCTCAAACCAATTTAAACTAACCATCAAAACCAAAACCAACACCAAAATCAAAGTCAAACCCCCACAACAGCCTAAATACTTGATATAAATTTAGTTTTCATGAGGCAATAAATAAATAAATATAGATTTTGAGGTTTAGAAAAATATATTGACAACAATATTTGAAATTTACTTGTTAATCAGATTATCTGCGGAAAATAATTTTTAAAAGGTAAGTATATAGAAACAAAGTGAGTAATATTTTTTTTAAGTAACTTAGCGACTAAAAATCCACTTAAGTGAATCCGAATCCGAGATTCAAACTCGACTCTTGAATAATACAATGTCCCTACCCATTGAACTATATTCACGGAGAGTCGGAGACAATATCTTTTTTATTATTGTGAAAAAAGAGAAAAACCCTTGATATGAATTTGTATTTTTTTACCGGATGAATTTGTTAATTTTTTTTGAGATAGATAGATAAAATGAAAAATAACCATTGAAATTCACACCAAATTTATACTATTTTATACTATTAATAAAATACTAGTAATATGAAAATTATTCAATTTCCTAATATTTCTAGCAGTTAATTAGTCAATTGAAACCGTGCATAGCACTTAAGCTAAGACAGAAATATAGTAAACGTTTTTATAAAATAGTCGTTGCCGACAAGTTGGTAGTGAAACCTTAAAAAGAAAATTAAGGATATACTCCACGAAAGAGATAGTAAGTGGTAGGTTCCTATAGGTGGTGTATGAGCATGACATCTGAAAATATACTTCAAGTTTAATGTGAAACTTACTACTTACTCAAAGTAGTTTTATAGTTTTGCGTTCATTTTTGTTAAAAAAGTTTGGCGTGAGTGGAAATATGGTGCCATTTAGCCTGGACTTGTGTAAGTATTGGTCCATAATGGACCAATATTTATAGTCATTGGATTATATGTAAACACAATAACACAATACACCTCATACATCTAATTATTTCCTTTACTCTTTCTAAAATATTTTCTTGCCTTCTCTCTCTTTCTGTAATTTCAACGTTCAATCTTCAATTGTTTTCAATGAAAACCCATAGTTTTCAATTAGTGGTGGAAATAGGTCAGGCCGTTCGACAGGGGCCTACGACCTAGCCTATTTAAAGCCTAACTCGGCCCAGCTCGTTTATTAACAAGGTCAGACTCAAGCTTTTTATAAGGCCTATTTAATTAAACAGACCAGGCTAAGGCTTGCAAAAAAGCCTATTAAGCCCATCAGGCCGGCCTATTAAGCTTTTTATTATAAAATATATTCCATTATTGATATTTCTGAAGTTTTTTTTCTTCATAAACACAAGCCTTCATATGATTGATTATTTAGTAGCTGATATATTGGTGTGATTAGACCTTCATAAGAATTCATCATTTAGTAGCAAACATATTTTTGTGCTTTAGGCCTTAATAAGTTTTGGCCTATTTAGAAGTAGGCTTACATGTGTTGTCAGACCTTAGCATAAAATAGGCTTTTAAATAGGCTTTCAGGCTTCATCAGCCCTTTTAAAAGGTCAGGCCAGACCTCGAAAATAAGCCTATGAGGGGTAATAGGCCAGACTCATGCCTATAATTTTTGAAGTAGGTCATGCTTAGGCCTTACAAAGCCCGGCCCGGCGTAGCCTATTTCCACCCCTATTTTCAATCAATGTTTAGAATTTAATAATTTAACATATCTTTTATATTATAATATTAAATTAATATTTACTCACAAAAAATTATTTTATATTTATTTTCATTTACTTAAACATAGCAATGAAAACCTTTTTTTTTAATAAAAGATATGAATGAACAGTTCAAGAATGTATTATTTATTATTACTGCTATTATTATTCTTTTGGAAAGGATAATATGACTATTTTTTGAAAAAAACAATGAAAATAAATAAATAATTGATAACGGTACCTGAACTATACTTTTCAGGTAACAATAACATGTATCTTTTTTCTTTCAAAAAAAATAACATGTACCTTTTCTTATTAAAAAAAAAATAACATGTACCTTTTCTCCCATTTGTGATAAAAATCATGTCCTTTTCTCTTTAAAAAAAAAACGTGTACGCTTTTGTAAAAATAAAATCAAATAAAAATATGCAGCTGTCCAACAATTACCGTAACAAATTTGTAAAAAAAAAAGTGTAAATACCAACAACAAAAAATTACGGTAACAAATTTGTCAAGAAAAAAAAACAATTACCGTAACAATTTGTAACATTTTAAATTATAATATTTATAAAGGATAAAAATGAAATTTTAATAAAATGATAAGAAAAGATGGGTTAGATTTAGTGTTTATAGACCATGATAAGATTTAGGGGTTCATATAATTTAACCATTGAAAAAATTGGTCCATCATGGACCAATATTTTCATGGGTTCATTTTATAAGGAACCTGGAAATATTTCTCGACTTTGATGCAAAGCAAGCTTTGACATGTTTGTTTGATTTTGTAAAAGCAAGCTAATTCGCAAGTTTCCAAGAATTTTCAAACTTTATAGTTCATAGACTTTGTTACTAACTTGACTTTAATGTTCTTTGTGCATGTTTTTCATGTGTTATGCAGTATAAGGTCAATTATTTCGGATAAGAAAGGCATATACGTAATTAATCTCTTTATTATCAAAAAAATTAATGTGTTAACGTGAATGTCGTGCAGGCCAAATCCATCTTAATGCTGGCAAATAGAATGAAGGTGCTAGAAAAAGTGACGACACCAATAGGGTCAGAACCATCAACACCAACAATAGTGCAATCTCCTCATCAATTATATAGCCCTGGACCAGGTCTTTCCATGAAAAGATCTTTGCAAAGGTTTCTACAAAAGAGAAAGAATAGGGTCCAAGAAGCATCTCCATATCATCGTTAG
Protein sequence

>MS.gene96249.t1

MRRNCNLELCLFPPYNNSSNHQNHPMVEEEDSNESTPMQNQQQPLTIFYDGKMCVTDVTELQAKSILMLANRMKVLEKVTTPIGSEPSTPTIVQSPHQLYSPGPGLSMKRSLQRFLQKRKNRVQEASPYHR