Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96387.t1 | RHN78864.1 | 92.8 | 97 | 7 | 0 | 1 | 97 | 1 | 97 | 1.20E-42 | 182.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96387.t1 | Q9M1K1 | 44.5 | 110 | 40 | 5 | 1 | 96 | 1 | 103 | 8.5e-10 | 64.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96387.t1 | I3SZM2 | 92.8 | 97 | 7 | 0 | 1 | 97 | 1 | 97 | 8.4e-43 | 182.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene96387.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96387.t1 | MTR_1g048720 | 91.753 | 97 | 8 | 0 | 1 | 97 | 1 | 97 | 5.51e-62 | 187 |
| MS.gene96387.t1 | MTR_1g048720 | 91.753 | 97 | 8 | 0 | 1 | 97 | 1 | 97 | 6.16e-62 | 187 |
| MS.gene96387.t1 | MTR_1g048720 | 91.753 | 97 | 8 | 0 | 1 | 97 | 1 | 97 | 8.28e-62 | 188 |
| MS.gene96387.t1 | MTR_1g048720 | 91.753 | 97 | 8 | 0 | 1 | 97 | 1 | 97 | 8.96e-62 | 188 |
| MS.gene96387.t1 | MTR_1g048710 | 84.536 | 97 | 7 | 1 | 1 | 97 | 1 | 89 | 1.88e-52 | 164 |
| MS.gene96387.t1 | MTR_1g048750 | 83.505 | 97 | 8 | 1 | 1 | 97 | 1 | 89 | 8.92e-52 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96387.t1 | AT3G56970 | 44.545 | 110 | 40 | 5 | 1 | 96 | 1 | 103 | 7.81e-16 | 70.1 |
| MS.gene96387.t1 | AT2G41240 | 40.196 | 102 | 46 | 3 | 1 | 96 | 1 | 93 | 1.72e-13 | 63.9 |
| MS.gene96387.t1 | AT2G41240 | 40.196 | 102 | 46 | 3 | 1 | 96 | 1 | 93 | 1.83e-13 | 63.9 |
| MS.gene96387.t1 | AT5G04150 | 72.500 | 40 | 8 | 1 | 60 | 96 | 78 | 117 | 6.68e-11 | 57.0 |
| MS.gene96387.t1 | AT5G04150 | 72.500 | 40 | 8 | 1 | 60 | 96 | 78 | 117 | 8.64e-11 | 56.6 |
Find 0 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAATTATTGATCTTCTTG+CGG | - | 11169:8988-9007 | MS.gene96387:intergenic | 25.0% |
| !!! | TAATTTTCTTGATCAAGTTG+AGG | + | 11169:8871-8890 | MS.gene96387:CDS | 25.0% |
| CATCCAATTGTTGAAAACAT+AGG | - | 11169:8779-8798 | MS.gene96387:intergenic | 30.0% | |
| ! | CTATGTTTTCAACAATTGGA+TGG | + | 11169:8777-8796 | MS.gene96387:CDS | 30.0% |
| CCAATTGTTGAAAACATAGG+AGG | - | 11169:8776-8795 | MS.gene96387:intergenic | 35.0% | |
| ! | CCTCCTATGTTTTCAACAAT+TGG | + | 11169:8773-8792 | MS.gene96387:CDS | 35.0% |
| TGTAGAATGAACTCTGATGC+TGG | - | 11169:8829-8848 | MS.gene96387:intergenic | 40.0% | |
| ! | ATGAACTCTGATGCTGGTTA+TGG | - | 11169:8823-8842 | MS.gene96387:intergenic | 40.0% |
| ! | TGAACTCTGATGCTGGTTAT+GGG | - | 11169:8822-8841 | MS.gene96387:intergenic | 40.0% |
| ! | TGATGCTGGTTATGGGTTAT+TGG | - | 11169:8815-8834 | MS.gene96387:intergenic | 40.0% |
| AACAATTGGATGGCCCTTTG+AGG | + | 11169:8787-8806 | MS.gene96387:CDS | 45.0% | |
| CAAGCTTCTTAACCATGCCA+AGG | - | 11169:8940-8959 | MS.gene96387:intergenic | 45.0% | |
| GATCGCGTTCACTAGCATTA+TGG | - | 11169:8964-8983 | MS.gene96387:intergenic | 45.0% | |
| ! | TCACTGCTTGTGGATTGTGA+GGG | - | 11169:8917-8936 | MS.gene96387:intergenic | 45.0% |
| !! | ATGGGTTATTGGCTCCTCAA+AGG | - | 11169:8804-8823 | MS.gene96387:intergenic | 45.0% |
| !! | TGGGTTATTGGCTCCTCAAA+GGG | - | 11169:8803-8822 | MS.gene96387:intergenic | 45.0% |
| CAATCCACAAGCAGTGACCT+TGG | + | 11169:8920-8939 | MS.gene96387:CDS | 50.0% | |
| ! | GTCACTGCTTGTGGATTGTG+AGG | - | 11169:8918-8937 | MS.gene96387:intergenic | 50.0% |
| CACAAGCAGTGACCTTGGCA+TGG | + | 11169:8925-8944 | MS.gene96387:CDS | 55.0% | |
| CATGCCAAGGTCACTGCTTG+TGG | - | 11169:8927-8946 | MS.gene96387:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11169 | gene | 8758 | 9048 | 8758 | ID=MS.gene96387 |
| 11169 | mRNA | 8758 | 9048 | 8758 | ID=MS.gene96387.t1;Parent=MS.gene96387 |
| 11169 | exon | 8758 | 9048 | 8758 | ID=MS.gene96387.t1.exon1;Parent=MS.gene96387.t1 |
| 11169 | CDS | 8758 | 9048 | 8758 | ID=cds.MS.gene96387.t1;Parent=MS.gene96387.t1 |
| Gene Sequence |
| Protein sequence |
