Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96556.t1 | AES92029.1 | 82.2 | 185 | 22 | 1 | 12 | 196 | 52 | 225 | 1.30E-85 | 325.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96556.t1 | G7JQ00 | 82.2 | 185 | 22 | 1 | 12 | 196 | 52 | 225 | 9.5e-86 | 325.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene96556.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96556.t1 | MTR_4g123470 | 82.162 | 185 | 22 | 1 | 12 | 196 | 52 | 225 | 1.74e-111 | 317 |
| MS.gene96556.t1 | MTR_7g113060 | 89.189 | 37 | 4 | 0 | 158 | 194 | 3 | 39 | 8.19e-17 | 75.1 |
| MS.gene96556.t1 | MTR_1g090753 | 40.964 | 83 | 49 | 0 | 12 | 94 | 66 | 148 | 1.70e-15 | 71.2 |
| MS.gene96556.t1 | MTR_7g025150 | 26.786 | 168 | 112 | 2 | 14 | 181 | 51 | 207 | 1.63e-12 | 64.3 |
| MS.gene96556.t1 | MTR_4g085790 | 26.190 | 168 | 113 | 2 | 14 | 181 | 51 | 207 | 7.53e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGTTCATGTAGAAAATC+AGG | 0.279397 | 7.3:-46456773 | MS.gene96556:intron |
| TTGAAGTTCCTATGTGGATA+TGG | 0.295769 | 7.3:-46456266 | MS.gene96556:CDS |
| AGGAATTTGCCGAAAGTATC+AGG | 0.335653 | 7.3:+46456120 | None:intergenic |
| ATCTACTTCCACCAAGGTAT+TGG | 0.372977 | 7.3:+46456379 | None:intergenic |
| CGATTATTCTACGAGTTTAG+TGG | 0.386306 | 7.3:-46456420 | MS.gene96556:CDS |
| TAGGAACTACCTGATACTTT+CGG | 0.400617 | 7.3:-46456129 | MS.gene96556:CDS |
| TCCGAACATCACATTTGGTA+TGG | 0.417548 | 7.3:+46456014 | None:intergenic |
| TCTACGAGTTTAGTGGAAGT+TGG | 0.437117 | 7.3:-46456413 | MS.gene96556:CDS |
| CCGAACATCACATTTGGTAT+GGG | 0.451435 | 7.3:+46456015 | None:intergenic |
| ATTATTGCTTTCCAATACCT+TGG | 0.456975 | 7.3:-46456390 | MS.gene96556:CDS |
| TGCCGCACTCATGGAATGAT+AGG | 0.462626 | 7.3:+46456308 | None:intergenic |
| TTGTGCTGCCAATGGTATTG+AGG | 0.463000 | 7.3:-46455974 | MS.gene96556:CDS |
| CCAAATGTGATGTTCGGAAA+AGG | 0.475440 | 7.3:-46456009 | MS.gene96556:CDS |
| CCCATACCAAATGTGATGTT+CGG | 0.477035 | 7.3:-46456015 | MS.gene96556:CDS |
| GTCATGTGTTTATCAACGCT+TGG | 0.483566 | 7.3:+46455891 | None:intergenic |
| GGCCGTTGTTGACAGATGGT+TGG | 0.487397 | 7.3:-46456452 | MS.gene96556:CDS |
| ACACGATGCTGCCGCACTCA+TGG | 0.487735 | 7.3:+46456299 | None:intergenic |
| TGTGCTGCCAATGGTATTGA+GGG | 0.501205 | 7.3:-46455973 | MS.gene96556:CDS |
| ACAGGAATGATATTGTCATA+AGG | 0.528831 | 7.3:+46456060 | None:intergenic |
| AGTCGGCCGTTGTTGACAGA+TGG | 0.531450 | 7.3:-46456456 | MS.gene96556:CDS |
| TGATAGGGTGGATAATCAGC+TGG | 0.541144 | 7.3:+46456324 | None:intergenic |
| CGAACATCACATTTGGTATG+GGG | 0.552019 | 7.3:+46456016 | None:intergenic |
| TCTGTATATTTGAGTCTCAC+AGG | 0.555272 | 7.3:+46456042 | None:intergenic |
| TTTCAAGTGCAAGTGCGTCA+TGG | 0.567746 | 7.3:-46455935 | MS.gene96556:CDS |
| ATTGCTTTCCAATACCTTGG+TGG | 0.575123 | 7.3:-46456387 | MS.gene96556:CDS |
| GTACCTTTGAAGTTCCTATG+TGG | 0.577838 | 7.3:-46456272 | MS.gene96556:CDS |
| GTCTCCGCCCTCAATACCAT+TGG | 0.581950 | 7.3:+46455966 | None:intergenic |
| TATCCACATAGGAACTTCAA+AGG | 0.582859 | 7.3:+46456269 | None:intergenic |
| TGACACTTGCTAGATGGTGA+TGG | 0.594754 | 7.3:-46456513 | MS.gene96556:intron |
| ATGTGATGTTCGGAAAAGGT+TGG | 0.606400 | 7.3:-46456005 | MS.gene96556:CDS |
| TGTTCAACAAATCAGTTAGT+CGG | 0.609582 | 7.3:-46456473 | MS.gene96556:CDS |
| GACACTTGCTAGATGGTGAT+GGG | 0.615365 | 7.3:-46456512 | MS.gene96556:intron |
| GCCGCACTCATGGAATGATA+GGG | 0.629158 | 7.3:+46456309 | None:intergenic |
| TCGTCGATCCATATCCACAT+AGG | 0.629580 | 7.3:+46456258 | None:intergenic |
| ACCCTATCATTCCATGAGTG+CGG | 0.640367 | 7.3:-46456310 | MS.gene96556:CDS |
| GCACTCATGGAATGATAGGG+TGG | 0.649652 | 7.3:+46456312 | None:intergenic |
| CATGATTACATCATGTTGTG+CGG | 0.656794 | 7.3:-46456084 | MS.gene96556:CDS |
| CTCCAACCATCTGTCAACAA+CGG | 0.679074 | 7.3:+46456450 | None:intergenic |
| TTTGAAATCTACTTCCACCA+AGG | 0.680517 | 7.3:+46456373 | None:intergenic |
| TGGATCGACGAAATCACCGA+AGG | 0.700849 | 7.3:-46456246 | MS.gene96556:CDS |
| GCTGCCAATGGTATTGAGGG+CGG | 0.739556 | 7.3:-46455970 | MS.gene96556:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTATATATGCTATAATAA+AGG | - | chr7.3:46456499-46456518 | MS.gene96556:CDS | 15.0% |
| !!! | CAGTTTTTTTTCTTTTTTTT+AGG | - | chr7.3:46456540-46456559 | MS.gene96556:intron | 15.0% |
| !! | AAAAAACAATAAACAACCTT+CGG | + | chr7.3:46456461-46456480 | None:intergenic | 20.0% |
| !! | TTCTATTTAAATACGATGAA+GGG | - | chr7.3:46455966-46455985 | MS.gene96556:CDS | 20.0% |
| !! | TTTCTATTTAAATACGATGA+AGG | - | chr7.3:46455965-46455984 | MS.gene96556:CDS | 20.0% |
| !!! | ATGAAAATTAAATCCTCTTT+TGG | - | chr7.3:46456011-46456030 | MS.gene96556:CDS | 20.0% |
| !!! | TAGACAGAGTATTAAAAAAA+AGG | + | chr7.3:46456087-46456106 | None:intergenic | 20.0% |
| ! | ATATTAAATCCAACCAAAAG+AGG | + | chr7.3:46456027-46456046 | None:intergenic | 25.0% |
| !! | AAATTAAATCCTCTTTTGGT+TGG | - | chr7.3:46456015-46456034 | MS.gene96556:CDS | 25.0% |
| !! | AATCATGATGTTTTACTCTA+AGG | + | chr7.3:46456591-46456610 | None:intergenic | 25.0% |
| !! | TTTTGATGCTATTCATTTAG+AGG | - | chr7.3:46456477-46456496 | MS.gene96556:CDS | 25.0% |
| ACAGGAATGATATTGTCATA+AGG | + | chr7.3:46456631-46456650 | None:intergenic | 30.0% | |
| ATTATTGCTTTCCAATACCT+TGG | - | chr7.3:46456298-46456317 | MS.gene96556:CDS | 30.0% | |
| TAATACTCTGTCTAACTTCT+AGG | - | chr7.3:46456093-46456112 | MS.gene96556:CDS | 30.0% | |
| TGTTCAACAAATCAGTTAGT+CGG | - | chr7.3:46456215-46456234 | MS.gene96556:CDS | 30.0% | |
| ! | TAAATATTTCTACGAGCTAC+CGG | - | chr7.3:46456147-46456166 | MS.gene96556:intron | 30.0% |
| CACAGTTCATGTAGAAAATC+AGG | - | chr7.3:46455915-46455934 | MS.gene96556:CDS | 35.0% | |
| CATGATTACATCATGTTGTG+CGG | - | chr7.3:46456604-46456623 | MS.gene96556:intron | 35.0% | |
| CGATTATTCTACGAGTTTAG+TGG | - | chr7.3:46456268-46456287 | MS.gene96556:CDS | 35.0% | |
| TAGGAACTACCTGATACTTT+CGG | - | chr7.3:46456559-46456578 | MS.gene96556:intron | 35.0% | |
| TATCCACATAGGAACTTCAA+AGG | + | chr7.3:46456422-46456441 | None:intergenic | 35.0% | |
| TCTGTATATTTGAGTCTCAC+AGG | + | chr7.3:46456649-46456668 | None:intergenic | 35.0% | |
| TTGAAGTTCCTATGTGGATA+TGG | - | chr7.3:46456422-46456441 | MS.gene96556:CDS | 35.0% | |
| ! | TTTGAAATCTACTTCCACCA+AGG | + | chr7.3:46456318-46456337 | None:intergenic | 35.0% |
| ATCTACTTCCACCAAGGTAT+TGG | + | chr7.3:46456312-46456331 | None:intergenic | 40.0% | |
| ATGTGATGTTCGGAAAAGGT+TGG | - | chr7.3:46456683-46456702 | MS.gene96556:intron | 40.0% | |
| ATTGCTTTCCAATACCTTGG+TGG | - | chr7.3:46456301-46456320 | MS.gene96556:CDS | 40.0% | |
| CCAAATGTGATGTTCGGAAA+AGG | - | chr7.3:46456679-46456698 | MS.gene96556:intron | 40.0% | |
| CCCATACCAAATGTGATGTT+CGG | - | chr7.3:46456673-46456692 | MS.gene96556:intron | 40.0% | |
| CCGAACATCACATTTGGTAT+GGG | + | chr7.3:46456676-46456695 | None:intergenic | 40.0% | |
| CGAACATCACATTTGGTATG+GGG | + | chr7.3:46456675-46456694 | None:intergenic | 40.0% | |
| TCCGAACATCACATTTGGTA+TGG | + | chr7.3:46456677-46456696 | None:intergenic | 40.0% | |
| TCTACGAGTTTAGTGGAAGT+TGG | - | chr7.3:46456275-46456294 | MS.gene96556:CDS | 40.0% | |
| ! | CCTTTTCCGAACATCACATT+TGG | + | chr7.3:46456682-46456701 | None:intergenic | 40.0% |
| !! | AGGAATTTGCCGAAAGTATC+AGG | + | chr7.3:46456571-46456590 | None:intergenic | 40.0% |
| !! | GTACCTTTGAAGTTCCTATG+TGG | - | chr7.3:46456416-46456435 | MS.gene96556:CDS | 40.0% |
| ACCCTATCATTCCATGAGTG+CGG | - | chr7.3:46456378-46456397 | MS.gene96556:CDS | 45.0% | |
| CTCCAACCATCTGTCAACAA+CGG | + | chr7.3:46456241-46456260 | None:intergenic | 45.0% | |
| TCGTCGATCCATATCCACAT+AGG | + | chr7.3:46456433-46456452 | None:intergenic | 45.0% | |
| TGATAGGGTGGATAATCAGC+TGG | + | chr7.3:46456367-46456386 | None:intergenic | 45.0% | |
| TTGTGCTGCCAATGGTATTG+AGG | - | chr7.3:46456714-46456733 | MS.gene96556:intron | 45.0% | |
| ! | ACGAGTTTTTGTGCTGCCAA+TGG | - | chr7.3:46456706-46456725 | MS.gene96556:intron | 45.0% |
| ! | GACACTTGCTAGATGGTGAT+GGG | - | chr7.3:46456176-46456195 | MS.gene96556:intron | 45.0% |
| ! | GAGCAATGACACTTGCTAGA+TGG | - | chr7.3:46456169-46456188 | MS.gene96556:intron | 45.0% |
| ! | TCTAGCAAGTGTCATTGCTC+CGG | + | chr7.3:46456169-46456188 | None:intergenic | 45.0% |
| ! | TGACACTTGCTAGATGGTGA+TGG | - | chr7.3:46456175-46456194 | MS.gene96556:intron | 45.0% |
| ! | TGTGCTGCCAATGGTATTGA+GGG | - | chr7.3:46456715-46456734 | MS.gene96556:intron | 45.0% |
| ! | TTTCAAGTGCAAGTGCGTCA+TGG | - | chr7.3:46456753-46456772 | MS.gene96556:intron | 45.0% |
| GCACTCATGGAATGATAGGG+TGG | + | chr7.3:46456379-46456398 | None:intergenic | 50.0% | |
| GCCGCACTCATGGAATGATA+GGG | + | chr7.3:46456382-46456401 | None:intergenic | 50.0% | |
| TGCCGCACTCATGGAATGAT+AGG | + | chr7.3:46456383-46456402 | None:intergenic | 50.0% | |
| TGGATCGACGAAATCACCGA+AGG | - | chr7.3:46456442-46456461 | MS.gene96556:CDS | 50.0% | |
| AGTCGGCCGTTGTTGACAGA+TGG | - | chr7.3:46456232-46456251 | MS.gene96556:CDS | 55.0% | |
| GGCCGTTGTTGACAGATGGT+TGG | - | chr7.3:46456236-46456255 | MS.gene96556:CDS | 55.0% | |
| GTCTCCGCCCTCAATACCAT+TGG | + | chr7.3:46456725-46456744 | None:intergenic | 55.0% | |
| ! | GCTGCCAATGGTATTGAGGG+CGG | - | chr7.3:46456718-46456737 | MS.gene96556:intron | 55.0% |
| ACACGATGCTGCCGCACTCA+TGG | + | chr7.3:46456392-46456411 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 46455903 | 46456807 | 46455903 | ID=MS.gene96556 |
| chr7.3 | mRNA | 46455903 | 46456807 | 46455903 | ID=MS.gene96556.t1;Parent=MS.gene96556 |
| chr7.3 | exon | 46456774 | 46456807 | 46456774 | ID=MS.gene96556.t1.exon1;Parent=MS.gene96556.t1 |
| chr7.3 | CDS | 46456774 | 46456807 | 46456774 | ID=cds.MS.gene96556.t1;Parent=MS.gene96556.t1 |
| chr7.3 | exon | 46456212 | 46456522 | 46456212 | ID=MS.gene96556.t1.exon2;Parent=MS.gene96556.t1 |
| chr7.3 | CDS | 46456212 | 46456522 | 46456212 | ID=cds.MS.gene96556.t1;Parent=MS.gene96556.t1 |
| chr7.3 | exon | 46455903 | 46456148 | 46455903 | ID=MS.gene96556.t1.exon3;Parent=MS.gene96556.t1 |
| chr7.3 | CDS | 46455903 | 46456148 | 46455903 | ID=cds.MS.gene96556.t1;Parent=MS.gene96556.t1 |
| Gene Sequence |
| Protein sequence |
