AlfalfaGEDB | MS.gene96588

M. sativa cultivar XinJiangDaYe / MS.gene96588

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96588.t1	RHN65248.1	98	50	1	0	1	50	117	166	4.20E-20	106.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96588.t1	Q9XIE1	70.2	47	14	0	1	47	71	117	7.2e-13	73.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96588.t1	A0A072V3R5	98.0	50	1	0	1	50	117	166	3.0e-20	106.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene96588.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96588.t1	MTR_3g006065	98.000	50	1	1	50	117	166	1.91e-30	104
MS.gene96588.t1	MTR_3g006150	97.727	44	1	1	44	117	160	1.27e-25	91.7
MS.gene96588.t1	MTR_8g062700	79.545	44	9	1	44	68	111	1.45e-17	73.9
MS.gene96588.t1	MTR_8g062700	79.545	44	9	1	44	68	111	1.49e-17	73.9
MS.gene96588.t1	MTR_5g036670	68.085	47	15	1	47	69	115	6.64e-16	69.3
MS.gene96588.t1	MTR_1g105960	75.000	40	10	1	40	27	66	1.74e-15	64.3
MS.gene96588.t1	MTR_1g105955	78.947	38	8	1	38	42	79	2.17e-15	65.9
MS.gene96588.t1	MTR_1g105955	70.000	40	12	1	40	121	160	1.25e-12	58.5
MS.gene96588.t1	MTR_6g008970	68.182	44	14	1	44	89	132	7.21e-14	63.5
MS.gene96588.t1	MTR_3g006185	65.306	49	17	2	50	126	174	1.06e-13	61.2
MS.gene96588.t1	MTR_3g006125	64.000	50	18	1	50	207	256	1.08e-13	62.4
MS.gene96588.t1	MTR_1g023820	67.500	40	13	5	44	17	56	5.13e-13	58.5
MS.gene96588.t1	MTR_3g006105	62.000	50	19	1	50	126	175	2.16e-12	58.2
MS.gene96588.t1	MTR_1g109550	60.000	40	16	1	40	41	80	7.04e-11	53.1
MS.gene96588.t1	MTR_6g008995	62.500	40	15	1	40	6	45	7.85e-11	55.1
MS.gene96588.t1	MTR_6g008995	62.500	40	15	1	40	63	102	9.57e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96588.t1	AT1G59890	70.213	47	14	1	47	71	117	3.69e-17	72.8
MS.gene96588.t1	AT1G59890	70.213	47	14	1	47	71	117	3.80e-17	72.8
MS.gene96588.t1	AT1G59890	70.213	47	14	1	47	71	117	3.80e-17	72.8
MS.gene96588.t1	AT1G59890	70.213	47	14	1	47	71	117	3.91e-17	72.8
MS.gene96588.t1	AT1G70060	67.347	49	16	1	49	40	88	6.63e-16	69.3
MS.gene96588.t1	AT1G70060	67.347	49	16	1	49	40	88	6.63e-16	69.3
MS.gene96588.t1	AT1G24190	63.830	47	17	1	47	40	86	1.92e-15	68.2
MS.gene96588.t1	AT1G24190	63.830	47	17	1	47	40	86	1.92e-15	68.2
MS.gene96588.t1	AT1G24190	63.830	47	17	1	47	40	86	2.00e-15	68.2
MS.gene96588.t1	AT1G10450	65.957	47	16	1	47	59	105	1.15e-14	65.9
MS.gene96588.t1	AT1G10450	65.957	47	16	1	47	59	105	1.15e-14	65.9
MS.gene96588.t1	AT1G10450	65.957	47	16	1	47	110	156	1.15e-14	65.9
MS.gene96588.t1	AT3G01320	70.455	44	13	1	44	83	126	1.74e-14	65.5
MS.gene96588.t1	AT3G01320	70.455	44	13	1	44	83	126	1.74e-14	65.5
MS.gene96588.t1	AT5G15020	68.182	44	14	1	44	78	121	3.56e-14	64.3
MS.gene96588.t1	AT5G15020	68.182	44	14	1	44	78	121	3.66e-14	64.3
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.16e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.16e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.16e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	266	314	8.49e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.49e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.49e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.56e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.74e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.74e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.82e-12	57.8
MS.gene96588.t1	AT4G12020	55.102	49	22	1	49	333	381	8.82e-12	57.8
MS.gene96588.t1	AT5G15025	63.636	44	16	1	44	73	116	9.86e-11	52.4

Find 11 sgRNAs with CRISPR-Local

Find 9 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGCATACAGATTTAATTT+TGG	0.178085	3.3:-1788047	MS.gene96588:CDS
AGAGTCAAGGATTTGTTTAA+AGG	0.255525	3.3:-1788067	MS.gene96588:CDS
GAGTCAAGGATTTGTTTAAA+GGG	0.376970	3.3:-1788066	MS.gene96588:CDS
AAAACTTATGAAGGATTTCA+AGG	0.385249	3.3:-1788122	None:intergenic
AGTGTGTTTCCATATGTCTT+AGG	0.412686	3.3:+1788007	None:intergenic
ATCTTCTTGCCTAAGACATA+TGG	0.434139	3.3:-1788016	MS.gene96588:CDS
AATTGATACAAGTGGTGTTG+CGG	0.577415	3.3:-1788092	MS.gene96588:CDS
CTTCGACCTCAGCTTGACAC+TGG	0.589307	3.3:-1787986	None:intergenic
TGGTGTTGCGGAAAGAGTCA+AGG	0.618099	3.3:-1788080	MS.gene96588:CDS
TACTAACCAGTGTCAAGCTG+AGG	0.644528	3.3:+1787980	None:intergenic
GCTTGTAGAATTGATACAAG+TGG	0.651698	3.3:-1788100	MS.gene96588:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAGTCAAGGATTTGTTTAA+AGG	-	chr3.3:1788035-1788054	MS.gene96588:CDS	30.0%
!	GAGTCAAGGATTTGTTTAAA+GGG	-	chr3.3:1788036-1788055	MS.gene96588:CDS	30.0%
!!	AGGGCATACAGATTTAATTT+TGG	-	chr3.3:1788055-1788074	MS.gene96588:CDS	30.0%
!!	GGGCATACAGATTTAATTTT+GGG	-	chr3.3:1788056-1788075	MS.gene96588:CDS	30.0%
	AGTGTGTTTCCATATGTCTT+AGG	+	chr3.3:1788098-1788117	None:intergenic	35.0%
	ATCTTCTTGCCTAAGACATA+TGG	-	chr3.3:1788086-1788105	MS.gene96588:CDS	35.0%
	GCTTGTAGAATTGATACAAG+TGG	-	chr3.3:1788002-1788021	MS.gene96588:CDS	35.0%
!	AATTGATACAAGTGGTGTTG+CGG	-	chr3.3:1788010-1788029	MS.gene96588:CDS	35.0%
!!	TGGTGTTGCGGAAAGAGTCA+AGG	-	chr3.3:1788022-1788041	MS.gene96588:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	1787987	1788137	1787987	ID=MS.gene96588
chr3.3	mRNA	1787987	1788137	1787987	ID=MS.gene96588.t1;Parent=MS.gene96588
chr3.3	exon	1787987	1788137	1787987	ID=MS.gene96588.t1.exon1;Parent=MS.gene96588.t1
chr3.3	CDS	1787987	1788137	1787987	ID=cds.MS.gene96588.t1;Parent=MS.gene96588.t1

Gene Sequence	>MS.gene96588 ATGAAGGATTTCAAGGCTTGTAGAATTGATACAAGTGGTGTTGCGGAAAGAGTCAAGGATTTGTTTAAAGGGCATACAGATTTAATTTTGGGATTCAACATCTTCTTGCCTAAGACATATGGAAACACACTTCGACCTCAGCTTGACACTG
Protein sequence	>MS.gene96588.t1 MKDFKACRIDTSGVAERVKDLFKGHTDLILGFNIFLPKTYGNTLRPQLDT