Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96662.t1 | AES97588.1 | 93 | 158 | 11 | 0 | 1 | 158 | 1 | 158 | 3.00E-80 | 307.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96662.t1 | G7K1J4 | 93.0 | 158 | 11 | 0 | 1 | 158 | 1 | 158 | 2.2e-80 | 307.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene96662.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96662.t1 | MTR_5g056450 | 93.038 | 158 | 11 | 0 | 1 | 158 | 1 | 158 | 4.78e-98 | 279 |
| MS.gene96662.t1 | MTR_1g041455 | 58.046 | 174 | 50 | 5 | 1 | 158 | 1 | 167 | 1.68e-58 | 179 |
| MS.gene96662.t1 | MTR_1g048880 | 47.953 | 171 | 58 | 7 | 1 | 158 | 1 | 153 | 3.68e-34 | 117 |
| MS.gene96662.t1 | MTR_1g075780 | 35.032 | 157 | 78 | 6 | 11 | 158 | 4 | 145 | 6.06e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATTGATTTCATTGATT+TGG | 0.224195 | 5.3:+45410380 | MS.gene96662:CDS |
| CATTTGAAGATTCTCCTTCT+AGG | 0.234818 | 5.3:-45410314 | None:intergenic |
| TGAGGAGGAAATTGTCCTTT+AGG | 0.244142 | 5.3:-45410153 | None:intergenic |
| AACCAAAACCAACGGTTATT+CGG | 0.300666 | 5.3:+45410208 | MS.gene96662:CDS |
| GAGGAGGAAATTGTCCTTTA+GGG | 0.320849 | 5.3:-45410152 | None:intergenic |
| AAATCCAATGGATTAACATC+TGG | 0.363954 | 5.3:-45410423 | None:intergenic |
| GGCCGAATAACCGTTGGTTT+TGG | 0.365772 | 5.3:-45410210 | None:intergenic |
| TGCTTCGATTGGTAAGCATT+TGG | 0.404776 | 5.3:-45410291 | None:intergenic |
| CTGGTGAGTTTAGTAATATT+AGG | 0.423811 | 5.3:+45409989 | MS.gene96662:CDS |
| TGAATAAAGCTTTCTGATTG+TGG | 0.433760 | 5.3:-45410115 | None:intergenic |
| TACCAATCGAAGCACCTAGA+AGG | 0.448280 | 5.3:+45410300 | MS.gene96662:CDS |
| CTCCTTCTAGGTGCTTCGAT+TGG | 0.488901 | 5.3:-45410302 | None:intergenic |
| GAAGGAGAATCTTCAAATGA+TGG | 0.491518 | 5.3:+45410318 | MS.gene96662:CDS |
| TAACCCAGATGTTAATCCAT+TGG | 0.496678 | 5.3:+45410419 | MS.gene96662:CDS |
| AATCCAATGGATTAACATCT+GGG | 0.500492 | 5.3:-45410422 | None:intergenic |
| ATCTTCAAATGATGGTACTA+AGG | 0.506473 | 5.3:+45410326 | MS.gene96662:CDS |
| GGCTGAGGCCGAATAACCGT+TGG | 0.511549 | 5.3:-45410216 | None:intergenic |
| AAAGGGTTTCTGCTTGGCTG+AGG | 0.532206 | 5.3:-45410231 | None:intergenic |
| TTCGATTGGTAAGCATTTGG+TGG | 0.535435 | 5.3:-45410288 | None:intergenic |
| CATGATGTGTCAAACTTGGT+AGG | 0.541015 | 5.3:+45410012 | MS.gene96662:CDS |
| TGCAACATCATCACCATGGC+TGG | 0.552771 | 5.3:+45409970 | None:intergenic |
| AATCAGAAAGCTTTATTCAA+CGG | 0.559288 | 5.3:+45410119 | MS.gene96662:CDS |
| TAGGCATGATGTGTCAAACT+TGG | 0.564079 | 5.3:+45410008 | MS.gene96662:CDS |
| AGCTTAAGTGATAAATCCAA+TGG | 0.575565 | 5.3:-45410435 | None:intergenic |
| CAATGTTTGCCACCACATGA+TGG | 0.576640 | 5.3:+45410174 | MS.gene96662:CDS |
| ACGGAGCGTATCAACCCTAA+AGG | 0.578711 | 5.3:+45410138 | MS.gene96662:CDS |
| AACTCACATCGAGTCTCACA+TGG | 0.586787 | 5.3:+45410080 | MS.gene96662:CDS |
| ATTACTAAACTCACCAGCCA+TGG | 0.594645 | 5.3:-45409983 | None:intergenic |
| TCATGTGGTGGCAAACATTG+AGG | 0.597472 | 5.3:-45410171 | None:intergenic |
| GCATTTGCCGATAACCAACA+TGG | 0.630189 | 5.3:-45410260 | None:intergenic |
| ATGATGTGTCAAACTTGGTA+GGG | 0.645237 | 5.3:+45410013 | MS.gene96662:CDS |
| TGTGGTGGCAAACATTGAGG+AGG | 0.673338 | 5.3:-45410168 | None:intergenic |
| GTCTCACATGGAGTACGAAG+AGG | 0.675983 | 5.3:+45410092 | MS.gene96662:CDS |
| AAAATCTTGCCATCATGTGG+TGG | 0.685328 | 5.3:-45410183 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGAAATCAATTTTCTTGAT+AGG | - | chr5.3:45410375-45410394 | None:intergenic | 20.0% |
| !!! | GAAAATTGATTTCATTGATT+TGG | + | chr5.3:45410380-45410399 | MS.gene96662:CDS | 20.0% |
| !!! | AATCAGAAAGCTTTATTCAA+CGG | + | chr5.3:45410119-45410138 | MS.gene96662:CDS | 25.0% |
| AGCTTAAGTGATAAATCCAA+TGG | - | chr5.3:45410438-45410457 | None:intergenic | 30.0% | |
| ATCTTCAAATGATGGTACTA+AGG | + | chr5.3:45410326-45410345 | MS.gene96662:CDS | 30.0% | |
| CTGGTGAGTTTAGTAATATT+AGG | + | chr5.3:45409989-45410008 | MS.gene96662:CDS | 30.0% | |
| TGAATAAAGCTTTCTGATTG+TGG | - | chr5.3:45410118-45410137 | None:intergenic | 30.0% | |
| TTTGAGTGTTTGTGAAAAAG+CGG | - | chr5.3:45410053-45410072 | None:intergenic | 30.0% | |
| !! | AAATCCAATGGATTAACATC+TGG | - | chr5.3:45410426-45410445 | None:intergenic | 30.0% |
| !! | AATCCAATGGATTAACATCT+GGG | - | chr5.3:45410425-45410444 | None:intergenic | 30.0% |
| !! | GATTTTTCAACCAAAACCAA+CGG | + | chr5.3:45410200-45410219 | MS.gene96662:CDS | 30.0% |
| AACCAAAACCAACGGTTATT+CGG | + | chr5.3:45410208-45410227 | MS.gene96662:CDS | 35.0% | |
| ATGATGTGTCAAACTTGGTA+GGG | + | chr5.3:45410013-45410032 | MS.gene96662:CDS | 35.0% | |
| CATTTGAAGATTCTCCTTCT+AGG | - | chr5.3:45410317-45410336 | None:intergenic | 35.0% | |
| GAAGGAGAATCTTCAAATGA+TGG | + | chr5.3:45410318-45410337 | MS.gene96662:CDS | 35.0% | |
| TAACCCAGATGTTAATCCAT+TGG | + | chr5.3:45410419-45410438 | MS.gene96662:CDS | 35.0% | |
| TGAAAAATCTTGCCATCATG+TGG | - | chr5.3:45410189-45410208 | None:intergenic | 35.0% | |
| AAAATCTTGCCATCATGTGG+TGG | - | chr5.3:45410186-45410205 | None:intergenic | 40.0% | |
| AACCAACATGGCCGAAAAAA+GGG | - | chr5.3:45410251-45410270 | None:intergenic | 40.0% | |
| CATGATGTGTCAAACTTGGT+AGG | + | chr5.3:45410012-45410031 | MS.gene96662:CDS | 40.0% | |
| GAGGAGGAAATTGTCCTTTA+GGG | - | chr5.3:45410155-45410174 | None:intergenic | 40.0% | |
| TAACCAACATGGCCGAAAAA+AGG | - | chr5.3:45410252-45410271 | None:intergenic | 40.0% | |
| TAGGCATGATGTGTCAAACT+TGG | + | chr5.3:45410008-45410027 | MS.gene96662:CDS | 40.0% | |
| TGAGGAGGAAATTGTCCTTT+AGG | - | chr5.3:45410156-45410175 | None:intergenic | 40.0% | |
| TGCTTCGATTGGTAAGCATT+TGG | - | chr5.3:45410294-45410313 | None:intergenic | 40.0% | |
| TTCGATTGGTAAGCATTTGG+TGG | - | chr5.3:45410291-45410310 | None:intergenic | 40.0% | |
| !! | TTTTCGGCCATGTTGGTTAT+CGG | + | chr5.3:45410253-45410272 | MS.gene96662:CDS | 40.0% |
| !!! | CCAAGCAGAAACCCTTTTTT+CGG | + | chr5.3:45410237-45410256 | MS.gene96662:CDS | 40.0% |
| AACTCACATCGAGTCTCACA+TGG | + | chr5.3:45410080-45410099 | MS.gene96662:CDS | 45.0% | |
| CAATGTTTGCCACCACATGA+TGG | + | chr5.3:45410174-45410193 | MS.gene96662:CDS | 45.0% | |
| CCGAAAAAAGGGTTTCTGCT+TGG | - | chr5.3:45410240-45410259 | None:intergenic | 45.0% | |
| GCATTTGCCGATAACCAACA+TGG | - | chr5.3:45410263-45410282 | None:intergenic | 45.0% | |
| TCATGTGGTGGCAAACATTG+AGG | - | chr5.3:45410174-45410193 | None:intergenic | 45.0% | |
| ! | TACCAATCGAAGCACCTAGA+AGG | + | chr5.3:45410300-45410319 | MS.gene96662:CDS | 45.0% |
| !!! | AACCCTTTTTTCGGCCATGT+TGG | + | chr5.3:45410246-45410265 | MS.gene96662:CDS | 45.0% |
| AAAGGGTTTCTGCTTGGCTG+AGG | - | chr5.3:45410234-45410253 | None:intergenic | 50.0% | |
| ACGGAGCGTATCAACCCTAA+AGG | + | chr5.3:45410138-45410157 | MS.gene96662:CDS | 50.0% | |
| CTCCTTCTAGGTGCTTCGAT+TGG | - | chr5.3:45410305-45410324 | None:intergenic | 50.0% | |
| GTCTCACATGGAGTACGAAG+AGG | + | chr5.3:45410092-45410111 | MS.gene96662:CDS | 50.0% | |
| TGTGGTGGCAAACATTGAGG+AGG | - | chr5.3:45410171-45410190 | None:intergenic | 50.0% | |
| !!! | GGCCGAATAACCGTTGGTTT+TGG | - | chr5.3:45410213-45410232 | None:intergenic | 50.0% |
| GGCTGAGGCCGAATAACCGT+TGG | - | chr5.3:45410219-45410238 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 45409985 | 45410461 | 45409985 | ID=MS.gene96662 |
| chr5.3 | mRNA | 45409985 | 45410461 | 45409985 | ID=MS.gene96662.t1;Parent=MS.gene96662 |
| chr5.3 | exon | 45409985 | 45410461 | 45409985 | ID=MS.gene96662.t1.exon1;Parent=MS.gene96662.t1 |
| chr5.3 | CDS | 45409985 | 45410461 | 45409985 | ID=cds.MS.gene96662.t1;Parent=MS.gene96662.t1 |
| Gene Sequence |
| Protein sequence |
