AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96884

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96884.t1 MTR_1g047060 63.529 85 31 0 112 196 3 87 7.64e-31 110
MS.gene96884.t1 MTR_6g477900 32.768 177 88 8 4 166 6 165 3.57e-17 78.6
MS.gene96884.t1 MTR_2g080010 32.727 165 88 6 4 161 15 163 5.30e-16 76.6
MS.gene96884.t1 MTR_4g089135 35.556 135 74 4 4 132 15 142 1.24e-14 72.8
MS.gene96884.t1 MTR_8g094580 32.298 161 82 7 4 147 7 157 1.62e-14 71.6
MS.gene96884.t1 MTR_3g096920 31.250 160 84 6 4 147 7 156 1.70e-14 71.6
MS.gene96884.t1 MTR_4g094302 28.481 158 89 6 4 147 11 158 2.42e-14 69.7
MS.gene96884.t1 MTR_7g100990 36.232 138 69 5 4 132 11 138 3.25e-14 71.2
MS.gene96884.t1 MTR_2g093810 32.704 159 83 5 4 145 14 165 3.57e-14 71.2
MS.gene96884.t1 MTR_4g081870 30.675 163 81 7 4 146 9 159 5.31e-14 70.1
MS.gene96884.t1 MTR_1g008740 31.847 157 89 6 2 146 25 175 5.70e-14 70.9
MS.gene96884.t1 MTR_1g069805 33.548 155 81 8 4 146 10 154 5.82e-14 70.1
MS.gene96884.t1 MTR_1g008740 31.847 157 89 6 2 146 11 161 6.71e-14 70.5
MS.gene96884.t1 MTR_3g096920 34.307 137 74 5 4 134 7 133 9.38e-14 70.1
MS.gene96884.t1 MTR_3g109340 31.210 157 89 6 2 146 20 169 1.20e-13 70.1
MS.gene96884.t1 MTR_4g094302 31.008 129 80 4 4 132 11 130 1.55e-13 66.6
MS.gene96884.t1 MTR_3g088110 32.500 160 82 7 4 147 7 156 2.42e-13 68.6
MS.gene96884.t1 MTR_6g084430 32.051 156 86 7 2 146 3 149 2.59e-13 68.9
MS.gene96884.t1 MTR_3g109340 30.769 156 90 6 2 146 20 168 3.08e-13 68.9
MS.gene96884.t1 MTR_3g096140 28.788 198 116 7 2 183 5 193 4.97e-13 67.4
MS.gene96884.t1 MTR_3g096140 31.250 160 86 6 2 146 5 155 9.73e-13 67.4
MS.gene96884.t1 MTR_8g023930 30.719 153 91 5 2 145 22 168 1.86e-12 66.6
MS.gene96884.t1 MTR_8g059170 30.818 159 84 7 4 146 14 162 3.28e-12 65.5
MS.gene96884.t1 MTR_3g093040 30.625 160 83 8 4 146 108 256 5.36e-12 64.7
MS.gene96884.t1 MTR_4g134460 33.553 152 82 7 6 146 56 199 5.42e-12 65.5
MS.gene96884.t1 MTR_2g079990 31.210 157 84 7 4 146 16 162 6.45e-12 64.7
MS.gene96884.t1 MTR_7g005280 31.613 155 83 6 4 146 15 158 9.22e-12 63.5
MS.gene96884.t1 MTR_1g090720 32.515 163 82 7 4 146 42 196 1.19e-11 64.3
MS.gene96884.t1 MTR_3g116070 30.612 147 90 5 4 144 23 163 1.43e-11 63.9
MS.gene96884.t1 MTR_8g023900 29.341 167 94 7 4 157 12 167 1.56e-11 63.9
MS.gene96884.t1 MTR_8g023900 26.961 204 126 9 7 198 196 388 8.47e-11 62.0
MS.gene96884.t1 MTR_6g012670 28.070 171 94 8 4 155 20 180 3.27e-11 62.8
MS.gene96884.t1 MTR_4g035590 30.719 153 90 7 4 146 8 154 3.39e-11 62.8
MS.gene96884.t1 MTR_1g097300 33.083 133 76 5 6 132 21 146 3.51e-11 63.2
MS.gene96884.t1 MTR_7g085220 27.749 191 97 9 4 169 8 182 4.32e-11 62.4
MS.gene96884.t1 MTR_2g079990 33.824 136 73 6 4 132 16 141 4.34e-11 62.4
MS.gene96884.t1 MTR_8g099750 32.143 140 74 5 4 134 14 141 4.40e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96884.t1 AT2G24430 34.395 157 84 6 2 146 14 163 1.21e-16 78.2
MS.gene96884.t1 AT2G24430 34.395 157 84 6 2 146 14 163 1.21e-16 78.2
MS.gene96884.t1 AT3G17730 32.484 157 84 7 4 146 6 154 1.98e-16 76.6
MS.gene96884.t1 AT3G10490 33.544 158 82 7 4 146 27 176 3.22e-16 75.9
MS.gene96884.t1 AT1G71930 30.573 157 92 5 4 149 9 159 1.77e-15 75.1
MS.gene96884.t1 AT1G71930 30.573 157 92 5 4 149 9 159 1.77e-15 75.1
MS.gene96884.t1 AT3G10490 33.544 158 82 7 4 146 27 176 2.42e-15 75.5
MS.gene96884.t1 AT4G36160 29.747 158 94 5 4 150 10 161 2.78e-15 74.7
MS.gene96884.t1 AT4G36160 29.747 158 94 5 4 150 10 161 2.78e-15 74.7
MS.gene96884.t1 AT4G36160 29.747 158 94 5 4 150 14 165 3.03e-15 74.7
MS.gene96884.t1 AT3G10480 33.544 158 82 7 4 146 27 176 8.21e-15 73.6
MS.gene96884.t1 AT3G10480 33.121 157 83 7 4 146 27 175 2.57e-14 72.4
MS.gene96884.t1 AT4G35580 29.814 161 95 6 1 149 6 160 3.24e-14 72.0
MS.gene96884.t1 AT4G35580 29.814 161 95 6 1 149 6 160 3.32e-14 72.0
MS.gene96884.t1 AT4G35580 29.814 161 95 6 1 149 6 160 3.36e-14 72.0
MS.gene96884.t1 AT1G61110 34.307 137 76 4 4 134 16 144 3.92e-14 71.2
MS.gene96884.t1 AT4G27410 30.864 162 88 7 4 151 14 165 7.34e-14 70.1
MS.gene96884.t1 AT5G39610 32.278 158 83 7 4 146 20 168 8.76e-14 69.7
MS.gene96884.t1 AT1G01720 32.919 161 81 7 4 147 7 157 1.15e-13 69.3
MS.gene96884.t1 AT5G64530 33.588 131 74 4 4 134 3 120 1.17e-13 67.8
MS.gene96884.t1 AT5G46590 32.484 157 84 7 4 145 6 155 1.69e-13 68.9
MS.gene96884.t1 AT5G64530 33.588 131 74 4 4 134 3 120 1.85e-13 66.6
MS.gene96884.t1 AT3G49530 29.114 158 95 6 1 146 10 162 2.01e-13 69.7
MS.gene96884.t1 AT3G49530 29.114 158 95 6 1 146 10 162 2.10e-13 69.3
MS.gene96884.t1 AT1G54330 32.026 153 87 6 4 146 6 151 2.32e-13 68.9
MS.gene96884.t1 AT4G28530 29.518 166 91 5 4 147 10 171 4.71e-13 68.2
MS.gene96884.t1 AT2G02450 33.544 158 86 7 3 149 50 199 5.36e-13 68.2
MS.gene96884.t1 AT2G02450 33.544 158 86 7 3 149 50 199 5.40e-13 68.2
MS.gene96884.t1 AT3G29035 33.333 138 77 5 4 135 24 152 5.50e-13 67.8
MS.gene96884.t1 AT3G18400 31.646 158 87 8 2 147 3 151 5.90e-13 67.8
MS.gene96884.t1 AT1G26870 33.548 155 88 5 2 149 14 160 6.38e-13 68.2
MS.gene96884.t1 AT3G15510 31.447 159 86 6 10 161 23 165 9.51e-13 67.4
MS.gene96884.t1 AT5G04410 30.128 156 89 6 4 146 9 157 1.18e-12 67.4
MS.gene96884.t1 AT4G10350 32.051 156 87 7 4 146 9 158 1.53e-12 66.6
MS.gene96884.t1 AT1G77450 31.250 160 84 6 4 147 10 159 2.02e-12 65.5
MS.gene96884.t1 AT5G46590 33.333 138 78 6 4 134 6 136 2.21e-12 65.9
MS.gene96884.t1 AT1G52890 29.936 157 86 7 4 146 14 160 2.93e-12 65.9
MS.gene96884.t1 AT2G43000 32.903 155 81 8 6 146 20 165 1.18e-11 63.5
MS.gene96884.t1 AT5G24590 30.380 158 87 7 4 146 13 162 1.20e-11 64.3
MS.gene96884.t1 AT3G10500 31.410 156 87 6 4 146 9 157 1.21e-11 64.7
MS.gene96884.t1 AT3G04060 30.818 159 85 8 4 146 20 169 5.28e-11 62.0
MS.gene96884.t1 AT5G04395 31.081 148 81 6 4 134 28 171 5.53e-11 60.5
MS.gene96884.t1 AT5G53950 26.368 201 125 6 2 196 15 198 7.33e-11 62.0

Find 64 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTTAAGCATGCACTTC+TGG 0.248003 1.4:+32450834 MS.gene96884:CDS
CAATGGTTCTGAGCTAATTA+AGG 0.256935 1.4:+32449378 MS.gene96884:CDS
GCTGGTGCTGGTGCTGCTGC+TGG 0.270068 1.4:+32449938 MS.gene96884:CDS
GCTGGTGCTGCTGCTGGTGC+TGG 0.319574 1.4:+32449944 MS.gene96884:CDS
GGAGCTGATAACGTGGTTCT+AGG 0.360842 1.4:+32449568 MS.gene96884:CDS
CGTGTGTTTGACTGCCCACC+AGG 0.412298 1.4:+32449815 MS.gene96884:CDS
AACACACGGCAACACACAAA+AGG 0.418206 1.4:-32449800 None:intergenic
GAAGTAAGGCGCAATTCAAA+TGG 0.422300 1.4:-32449424 None:intergenic
GAAGAGGTTCAGAATTTCAA+TGG 0.424440 1.4:+32449361 MS.gene96884:CDS
AGGTGATGCATCGTATTTGC+AGG 0.428163 1.4:+32450000 MS.gene96884:CDS
GGTACTGTAAGACAGGATAT+TGG 0.432912 1.4:+32449512 MS.gene96884:CDS
GCTGGTCCTGCTGTTGATGA+TGG 0.434280 1.4:+32449962 MS.gene96884:CDS
TGGCGTTGGCTTCGTCCCTT+CGG 0.444128 1.4:+32449297 MS.gene96884:CDS
TGATGTCACTCAGAAACCTA+TGG 0.460864 1.4:+32449907 MS.gene96884:CDS
GCCTTCTTCAACATCCGTTA+AGG 0.461031 1.4:-32449864 None:intergenic
GCAATTCAAATGGGTCATAC+TGG 0.471306 1.4:-32449414 None:intergenic
CCTCCTCTGCTCATCAACTT+TGG 0.480636 1.4:-32449491 None:intergenic
CAGAGGAGGTACTGTAAGAC+AGG 0.483813 1.4:+32449505 MS.gene96884:CDS
CAAATGGGTCATACTGGTAC+AGG 0.492656 1.4:-32449408 None:intergenic
GAATTGCTTCCACCTGGCGT+TGG 0.493230 1.4:+32449283 MS.gene96884:CDS
CCACATGGACGCTAGGACAC+CGG 0.500540 1.4:+32450810 MS.gene96884:CDS
ATGGATCTGAAAGGTGAGTT+CGG 0.504576 1.4:+32449839 MS.gene96884:CDS
CCTTGGGTGACGGAAGGAAA+AGG 0.509656 1.4:-32449449 None:intergenic
GTGAAGCAGTAAAATTCCCT+TGG 0.511128 1.4:-32449466 None:intergenic
GATGTCACTCAGAAACCTAT+GGG 0.511266 1.4:+32449908 MS.gene96884:CDS
GACAGGATATTGGATGCAGA+AGG 0.514396 1.4:+32449522 MS.gene96884:CDS
AGGTGAGTTCGGAGCCTTAA+CGG 0.523642 1.4:+32449850 MS.gene96884:CDS
AGTGCATGCTTAAGCTGCTC+CGG 0.526169 1.4:-32450829 None:intergenic
GAAGGGACGAAGCCAACGCC+AGG 0.531335 1.4:-32449295 None:intergenic
AGTAAAATTCCCTTGGGTGA+CGG 0.539767 1.4:-32449459 None:intergenic
TGGACGTTGTCAAAACTAGC+TGG 0.540827 1.4:+32449632 MS.gene96884:CDS
CACTCTTGCTAACAAGCCTC+AGG 0.544263 1.4:+32449669 MS.gene96884:CDS
GTTGAAGAAGGCGATGCTGC+TGG 0.544550 1.4:+32449875 MS.gene96884:CDS
AAGTAAGGCGCAATTCAAAT+GGG 0.549492 1.4:-32449423 None:intergenic
GCCTTAACGGATGTTGAAGA+AGG 0.552951 1.4:+32449863 MS.gene96884:CDS
GCTATTCGAGACATTACCGT+TGG 0.557631 1.4:+32449547 MS.gene96884:CDS
TGAAGCAGTAAAATTCCCTT+GGG 0.559532 1.4:-32449465 None:intergenic
TTGTCTTCCACATGGACGCT+AGG 0.559553 1.4:+32450803 MS.gene96884:CDS
TGGCAAGGTAGTGGCTAACA+AGG 0.563537 1.4:-32449327 None:intergenic
GTTTGACTGCCCACCAGGGA+TGG 0.566884 1.4:+32449820 MS.gene96884:CDS
GGAAGGAAAAGGATGAAGTA+AGG 0.567017 1.4:-32449438 None:intergenic
CCTTTCAGATCCATCCCTGG+TGG 0.578274 1.4:-32449830 None:intergenic
TCACCTTTCAGATCCATCCC+TGG 0.585698 1.4:-32449833 None:intergenic
GCACAACCATCATCAACAGC+AGG 0.592957 1.4:-32449968 None:intergenic
CTTTCAGATCCATCCCTGGT+GGG 0.593720 1.4:-32449829 None:intergenic
GCAATGGAATTGCTTCCACC+TGG 0.596598 1.4:+32449277 None:intergenic
AAATTCCCTTGGGTGACGGA+AGG 0.600300 1.4:-32449455 None:intergenic
GGGACGAAGCCAACGCCAGG+TGG 0.602159 1.4:-32449292 None:intergenic
GATGGTTGTGCTGCTGAGCA+AGG 0.608689 1.4:+32449980 MS.gene96884:CDS
CCAAAGTTGATGAGCAGAGG+AGG 0.609229 1.4:+32449491 MS.gene96884:CDS
ACAGGATATTGGATGCAGAA+GGG 0.612256 1.4:+32449523 MS.gene96884:CDS
CTATTTAGGAAATTCACCGA+TGG 0.613156 1.4:+32449612 MS.gene96884:CDS
CTGCCAAAGTTGATGAGCAG+AGG 0.624165 1.4:+32449488 MS.gene96884:CDS
CCACCAGGGATGGATCTGAA+AGG 0.624529 1.4:+32449830 MS.gene96884:CDS
AACCACGTTATCAGCTCCAA+CGG 0.626095 1.4:-32449563 None:intergenic
TACCGTTGGAGCTGATAACG+TGG 0.651576 1.4:+32449561 MS.gene96884:CDS
TGACTGTGTTGTCTTCCACA+TGG 0.651853 1.4:+32450795 MS.gene96884:CDS
CTGGTGGGCAGTCAAACACA+CGG 0.652222 1.4:-32449814 None:intergenic
CCGGTGTCCTAGCGTCCATG+TGG 0.665425 1.4:-32450810 None:intergenic
TAACAAGGAGTTCATCCGAA+GGG 0.665782 1.4:-32449312 None:intergenic
ATGTCACTCAGAAACCTATG+GGG 0.683258 1.4:+32449909 MS.gene96884:CDS
GTGTGTTTGACTGCCCACCA+GGG 0.701217 1.4:+32449816 MS.gene96884:CDS
CTAACAAGGAGTTCATCCGA+AGG 0.706470 1.4:-32449313 None:intergenic
TGTCACTCAGAAACCTATGG+GGG 0.731150 1.4:+32449910 MS.gene96884:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTTATAAAAAAAAA+AGG - chr1.4:32450616-32450635 None:intergenic 0.0%
!! ATCAATATTAATTAATCTAT+TGG + chr1.4:32449736-32449755 MS.gene96884:intron 10.0%
!! TTTAAAAAATGGTTTAATTA+GGG + chr1.4:32450375-32450394 MS.gene96884:intron 10.0%
!!! TTTTAAAAAATGGTTTAATT+AGG + chr1.4:32450374-32450393 MS.gene96884:intron 10.0%
!!! TTTTTGAAATTTTAAAGAAT+CGG - chr1.4:32450450-32450469 None:intergenic 10.0%
!! GATAAAATTTATCAAAACTT+TGG + chr1.4:32450265-32450284 MS.gene96884:intron 15.0%
!! TTATATATAAATAATTGTGC+AGG + chr1.4:32449775-32449794 MS.gene96884:intron 15.0%
!!! GGAGTTTATTTTTTAAAAAA+TGG + chr1.4:32450364-32450383 MS.gene96884:intron 15.0%
!! AAATAATGATGAATTTGTCA+TGG - chr1.4:32450640-32450659 None:intergenic 20.0%
!!! AAAATAAACTCCGAATTTTT+TGG - chr1.4:32450356-32450375 None:intergenic 20.0%
!!! CAACTTTTCATTTCATTTTA+TGG + chr1.4:32450691-32450710 MS.gene96884:intron 20.0%
! ATATGTATTTGACTGCTATT+GGG + chr1.4:32450122-32450141 MS.gene96884:intron 25.0%
! TGATGAAATAACGAACAAAA+GGG - chr1.4:32449720-32449739 None:intergenic 25.0%
! TTGATGAAATAACGAACAAA+AGG - chr1.4:32449721-32449740 None:intergenic 25.0%
!! ATTTTATGGTAGAGATAAGA+AGG + chr1.4:32450705-32450724 MS.gene96884:intron 25.0%
!! TCTCTCTTCTTTTTCTATTT+AGG + chr1.4:32449598-32449617 MS.gene96884:CDS 25.0%
!!! GTTTTGTTTTGATGTTTATC+AGG + chr1.4:32450753-32450772 MS.gene96884:intron 25.0%
AACGACCACGATTTAAAAAT+TGG - chr1.4:32450215-32450234 None:intergenic 30.0%
ACATGTTACAATCACATGAA+TGG - chr1.4:32450487-32450506 None:intergenic 30.0%
CATATGTATTTGACTGCTAT+TGG + chr1.4:32450121-32450140 MS.gene96884:intron 30.0%
CATCAAAATCATTGATGTTG+TGG - chr1.4:32450183-32450202 None:intergenic 30.0%
TTGGCTTCTTCCAAAAAATT+CGG + chr1.4:32450343-32450362 MS.gene96884:intron 30.0%
! AATTTTTAAATCGTGGTCGT+TGG + chr1.4:32450214-32450233 MS.gene96884:intron 30.0%
! CTCATTAGTGTCAATTTTGT+GGG + chr1.4:32450315-32450334 MS.gene96884:intron 30.0%
! TATGGCCAATTTTTAAATCG+TGG + chr1.4:32450207-32450226 MS.gene96884:intron 30.0%
! TCTCATTAGTGTCAATTTTG+TGG + chr1.4:32450314-32450333 MS.gene96884:intron 30.0%
!! CAATGATTTTGATGTCTGTA+TGG + chr1.4:32450189-32450208 MS.gene96884:intron 30.0%
!!! AACCTCTTCATTGTTTTTGT+TGG - chr1.4:32449350-32449369 None:intergenic 30.0%
AAAATGATGCGCTTGAATGT+GGG + chr1.4:32450157-32450176 MS.gene96884:intron 35.0%
AAGTAAGGCGCAATTCAAAT+GGG - chr1.4:32449426-32449445 None:intergenic 35.0%
CTATTTAGGAAATTCACCGA+TGG + chr1.4:32449612-32449631 MS.gene96884:CDS 35.0%
GAAATAACGAACAAAAGGGA+AGG - chr1.4:32449716-32449735 None:intergenic 35.0%
GAAGAGGTTCAGAATTTCAA+TGG + chr1.4:32449361-32449380 MS.gene96884:CDS 35.0%
TAAAATGATGCGCTTGAATG+TGG + chr1.4:32450156-32450175 MS.gene96884:intron 35.0%
TGAAGCAGTAAAATTCCCTT+GGG - chr1.4:32449468-32449487 None:intergenic 35.0%
TGCCAACAAAAACAATGAAG+AGG + chr1.4:32449345-32449364 MS.gene96884:CDS 35.0%
TTTCCCAAATACAATACCTG+AGG - chr1.4:32449688-32449707 None:intergenic 35.0%
! AAGCCTCAGGTATTGTATTT+GGG + chr1.4:32449682-32449701 MS.gene96884:intron 35.0%
! GTCAATTTTGTGGGATAACT+TGG + chr1.4:32450324-32450343 MS.gene96884:intron 35.0%
!! CAATGGTTCTGAGCTAATTA+AGG + chr1.4:32449378-32449397 MS.gene96884:CDS 35.0%
!!! CTTCATTGTTTTTGTTGGCA+AGG - chr1.4:32449345-32449364 None:intergenic 35.0%
ACAGGATATTGGATGCAGAA+GGG + chr1.4:32449523-32449542 MS.gene96884:CDS 40.0%
AGGACGTGATTTGAAGAATC+TGG - chr1.4:32450596-32450615 None:intergenic 40.0%
AGTAAAATTCCCTTGGGTGA+CGG - chr1.4:32449462-32449481 None:intergenic 40.0%
ATGGATCTGAAAGGTGAGTT+CGG + chr1.4:32449839-32449858 MS.gene96884:CDS 40.0%
ATGTCACTCAGAAACCTATG+GGG + chr1.4:32449909-32449928 MS.gene96884:CDS 40.0%
GAAGTAAGGCGCAATTCAAA+TGG - chr1.4:32449427-32449446 None:intergenic 40.0%
GATGTCACTCAGAAACCTAT+GGG + chr1.4:32449908-32449927 MS.gene96884:CDS 40.0%
GCAATTCAAATGGGTCATAC+TGG - chr1.4:32449417-32449436 None:intergenic 40.0%
GGAAGGAAAAGGATGAAGTA+AGG - chr1.4:32449441-32449460 None:intergenic 40.0%
GGTACTGTAAGACAGGATAT+TGG + chr1.4:32449512-32449531 MS.gene96884:CDS 40.0%
GTGAAGCAGTAAAATTCCCT+TGG - chr1.4:32449469-32449488 None:intergenic 40.0%
TAACAAGGAGTTCATCCGAA+GGG - chr1.4:32449315-32449334 None:intergenic 40.0%
TGATGTCACTCAGAAACCTA+TGG + chr1.4:32449907-32449926 MS.gene96884:CDS 40.0%
! CAAGCCTCAGGTATTGTATT+TGG + chr1.4:32449681-32449700 MS.gene96884:intron 40.0%
!! TGTTTTTGTTGGCAAGGTAG+TGG - chr1.4:32449339-32449358 None:intergenic 40.0%
!!! CTAGTTTTGACAACGTCCAT+CGG - chr1.4:32449631-32449650 None:intergenic 40.0%
AACACACGGCAACACACAAA+AGG - chr1.4:32449803-32449822 None:intergenic 45.0%
AACCACGTTATCAGCTCCAA+CGG - chr1.4:32449566-32449585 None:intergenic 45.0%
AGGTGATGCATCGTATTTGC+AGG + chr1.4:32450000-32450019 MS.gene96884:CDS 45.0%
CAAATGGGTCATACTGGTAC+AGG - chr1.4:32449411-32449430 None:intergenic 45.0%
CTAACAAGGAGTTCATCCGA+AGG - chr1.4:32449316-32449335 None:intergenic 45.0%
GACAGGATATTGGATGCAGA+AGG + chr1.4:32449522-32449541 MS.gene96884:CDS 45.0%
GCCTTCTTCAACATCCGTTA+AGG - chr1.4:32449867-32449886 None:intergenic 45.0%
GCTATTCGAGACATTACCGT+TGG + chr1.4:32449547-32449566 MS.gene96884:CDS 45.0%
TGACTGTGTTGTCTTCCACA+TGG + chr1.4:32450795-32450814 MS.gene96884:CDS 45.0%
TGGACGTTGTCAAAACTAGC+TGG + chr1.4:32449632-32449651 MS.gene96884:CDS 45.0%
TGTCACTCAGAAACCTATGG+GGG + chr1.4:32449910-32449929 MS.gene96884:CDS 45.0%
! GCCTTAACGGATGTTGAAGA+AGG + chr1.4:32449863-32449882 MS.gene96884:CDS 45.0%
AGGTGAGTTCGGAGCCTTAA+CGG + chr1.4:32449850-32449869 MS.gene96884:CDS 50.0%
AGTGCATGCTTAAGCTGCTC+CGG - chr1.4:32450832-32450851 None:intergenic 50.0%
CACTCTTGCTAACAAGCCTC+AGG + chr1.4:32449669-32449688 MS.gene96884:CDS 50.0%
CCTCCTCTGCTCATCAACTT+TGG - chr1.4:32449494-32449513 None:intergenic 50.0%
CTTTCAGATCCATCCCTGGT+GGG - chr1.4:32449832-32449851 None:intergenic 50.0%
GCACAACCATCATCAACAGC+AGG - chr1.4:32449971-32449990 None:intergenic 50.0%
GCAGCTTAAGCATGCACTTC+TGG + chr1.4:32450834-32450853 MS.gene96884:CDS 50.0%
TCACCTTTCAGATCCATCCC+TGG - chr1.4:32449836-32449855 None:intergenic 50.0%
TTGTCTTCCACATGGACGCT+AGG + chr1.4:32450803-32450822 MS.gene96884:CDS 50.0%
! CTTTTCCTTCCGTCACCCAA+GGG + chr1.4:32449450-32449469 MS.gene96884:CDS 50.0%
! GGAGCTGATAACGTGGTTCT+AGG + chr1.4:32449568-32449587 MS.gene96884:CDS 50.0%
! TACCGTTGGAGCTGATAACG+TGG + chr1.4:32449561-32449580 MS.gene96884:CDS 50.0%
! TGGCAAGGTAGTGGCTAACA+AGG - chr1.4:32449330-32449349 None:intergenic 50.0%
!! AAATTCCCTTGGGTGACGGA+AGG - chr1.4:32449458-32449477 None:intergenic 50.0%
!! CAGAGGAGGTACTGTAAGAC+AGG + chr1.4:32449505-32449524 MS.gene96884:CDS 50.0%
!! CCAAAGTTGATGAGCAGAGG+AGG + chr1.4:32449491-32449510 MS.gene96884:CDS 50.0%
!! CTGCCAAAGTTGATGAGCAG+AGG + chr1.4:32449488-32449507 MS.gene96884:CDS 50.0%
ACTCAGAAACCTATGGGGGC+TGG + chr1.4:32449914-32449933 MS.gene96884:CDS 55.0%
CCACCAGGGATGGATCTGAA+AGG + chr1.4:32449830-32449849 MS.gene96884:CDS 55.0%
CCTTTCAGATCCATCCCTGG+TGG - chr1.4:32449833-32449852 None:intergenic 55.0%
GAATTGCTTCCACCTGGCGT+TGG + chr1.4:32449283-32449302 MS.gene96884:CDS 55.0%
GTGTGTTTGACTGCCCACCA+GGG + chr1.4:32449816-32449835 MS.gene96884:CDS 55.0%
GTTGAAGAAGGCGATGCTGC+TGG + chr1.4:32449875-32449894 MS.gene96884:CDS 55.0%
! CCTTTTCCTTCCGTCACCCA+AGG + chr1.4:32449449-32449468 MS.gene96884:CDS 55.0%
! CTGGTGGGCAGTCAAACACA+CGG - chr1.4:32449817-32449836 None:intergenic 55.0%
! GATGGTTGTGCTGCTGAGCA+AGG + chr1.4:32449980-32449999 MS.gene96884:CDS 55.0%
! GCTGGTCCTGCTGTTGATGA+TGG + chr1.4:32449962-32449981 MS.gene96884:CDS 55.0%
!! CCTTGGGTGACGGAAGGAAA+AGG - chr1.4:32449452-32449471 None:intergenic 55.0%
CCACATGGACGCTAGGACAC+CGG + chr1.4:32450810-32450829 MS.gene96884:CDS 60.0%
CGTGTGTTTGACTGCCCACC+AGG + chr1.4:32449815-32449834 MS.gene96884:CDS 60.0%
GTTTGACTGCCCACCAGGGA+TGG + chr1.4:32449820-32449839 MS.gene96884:CDS 60.0%
! AAACCTATGGGGGCTGGTGC+TGG + chr1.4:32449920-32449939 MS.gene96884:CDS 60.0%
!! TGGCGTTGGCTTCGTCCCTT+CGG + chr1.4:32449297-32449316 MS.gene96884:CDS 60.0%
CCGGTGTCCTAGCGTCCATG+TGG - chr1.4:32450813-32450832 None:intergenic 65.0%
GAAGGGACGAAGCCAACGCC+AGG - chr1.4:32449298-32449317 None:intergenic 65.0%
GCACCAGCACCAGCCCCCAT+AGG - chr1.4:32449926-32449945 None:intergenic 70.0%
GGGACGAAGCCAACGCCAGG+TGG - chr1.4:32449295-32449314 None:intergenic 70.0%
!! ATGGGGGCTGGTGCTGGTGC+TGG + chr1.4:32449926-32449945 MS.gene96884:CDS 70.0%
!! GCTGGTGCTGCTGCTGGTGC+TGG + chr1.4:32449944-32449963 MS.gene96884:CDS 70.0%
!! GCTGGTGCTGGTGCTGCTGC+TGG + chr1.4:32449938-32449957 MS.gene96884:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 32449280 32450891 32449280 ID=MS.gene96884
chr1.4 mRNA 32449280 32450891 32449280 ID=MS.gene96884.t1;Parent=MS.gene96884
chr1.4 exon 32449280 32449690 32449280 ID=MS.gene96884.t1.exon1;Parent=MS.gene96884.t1
chr1.4 CDS 32449280 32449690 32449280 ID=cds.MS.gene96884.t1;Parent=MS.gene96884.t1
chr1.4 exon 32449797 32450021 32449797 ID=MS.gene96884.t1.exon2;Parent=MS.gene96884.t1
chr1.4 CDS 32449797 32450021 32449797 ID=cds.MS.gene96884.t1;Parent=MS.gene96884.t1
chr1.4 exon 32450775 32450891 32450775 ID=MS.gene96884.t1.exon3;Parent=MS.gene96884.t1
chr1.4 CDS 32450775 32450891 32450775 ID=cds.MS.gene96884.t1;Parent=MS.gene96884.t1
Gene Sequence

>MS.gene96884

ATGGAATTGCTTCCACCTGGCGTTGGCTTCGTCCCTTCGGATGAACTCCTTGTTAGCCACTACCTTGCCAACAAAAACAATGAAGAGGTTCAGAATTTCAATGGTTCTGAGCTAATTAAGGAGCTGAACCTGTACCAGTATGACCCATTTGAATTGCGCCTTACTTCATCCTTTTCCTTCCGTCACCCAAGGGAATTTTACTGCTTCACTGCCAAAGTTGATGAGCAGAGGAGGTACTGTAAGACAGGATATTGGATGCAGAAGGGAGCTATTCGAGACATTACCGTTGGAGCTGATAACGTGGTTCTAGGAAAAAAGTCTCTCTTCTTTTTCTATTTAGGAAATTCACCGATGGACGTTGTCAAAACTAGCTGGACTATGTACGAGTACACTCTTGCTAACAAGCCTCAGGTATTGTATTTGGGAAATCTAACCTTCCCTTTTGTTCGTTATTTCATCAATATTAATTAATCTATTGGTAACTACTAACTATTTTTATATATAAATAATTGTGCAGGCTCCTTTTGTGTGTTGCCGTGTGTTTGACTGCCCACCAGGGATGGATCTGAAAGGTGAGTTCGGAGCCTTAACGGATGTTGAAGAAGGCGATGCTGCTGGCGATGACTCTGATGTCACTCAGAAACCTATGGGGGCTGGTGCTGGTGCTGGTGCTGCTGCTGGTGCTGGTCCTGCTGTTGATGATGGTTGTGCTGCTGAGCAAGGTGATGCATCGTATTTGCAGGTAAAATATATTAAATTTTGTTCTCTCTTTATATTTTGTTGTCAGTTAGAATTTTAGAATAGTTGAATGTGTCATAAATCATAATTTTTTTTTGTGTTGCATATGTATTTGACTGCTATTGGGCATAAGTTTAATAAAATGATGCGCTTGAATGTGGGCCACAACATCAATGATTTTGATGTCTGTATGGCCAATTTTTAAATCGTGGTCGTTGGCTATTGCATCACAAACATTGACAGTGTAGATAAAATTTATCAAAACTTTGGTTTAATTTCAAGTTTTCATATTAGATTCTCATTAGTGTCAATTTTGTGGGATAACTTGGCTTCTTCCAAAAAATTCGGAGTTTATTTTTTAAAAAATGGTTTAATTAGGGATGTTATTGTTAAGAGTCAATCAATTAACGTTGTTGATGTTCTTTGCAACCGATTCTTTAAAATTTCAAAAAATTTACAACTTAATCCATTCATGTGATTGTAACATGTCAAAATGTCATCTTGTGTGTAGAAAATTAAGTGATAGTAATTCTCAAAACATATAGCAATTGATTAAGTTAACGTAGCTATTCTTACCAGATTCTTCAAATCACGTCCTTTTTTTTTTATAAATTTTTTGCCATGACAAATTCATCATTATTTTTATTGTTGTTGTTCTCACTTTTTGAAAATGCAACTTTTCATTTCATTTTATGGTAGAGATAAGAAGGAATTGTTGATGTATTGATTTGCTTTGTTTTGTTTTGATGTTTATCAGGCGAGTTATGCTGCATTTTTTGACTGTGTTGTCTTCCACATGGACGCTAGGACACCGGAGCAGCTTAAGCATGCACTTCTGGACGAAGATTTTCTTGAACTCGCTGATTTTGTATAA
Protein sequence

>MS.gene96884.t1

MELLPPGVGFVPSDELLVSHYLANKNNEEVQNFNGSELIKELNLYQYDPFELRLTSSFSFRHPREFYCFTAKVDEQRRYCKTGYWMQKGAIRDITVGADNVVLGKKSLFFFYLGNSPMDVVKTSWTMYEYTLANKPQAPFVCCRVFDCPPGMDLKGEFGALTDVEEGDAAGDDSDVTQKPMGAGAGAGAAAGAGPAVDDGCAAEQGDASYLQASYAAFFDCVVFHMDARTPEQLKHALLDEDFLELADFV