AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96889

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96889.t1 MTR_1g047550 88.095 126 15 0 18 143 1 126 6.51e-80 234
MS.gene96889.t1 MTR_8g079502 83.333 126 21 0 18 143 1 126 3.26e-74 219
MS.gene96889.t1 MTR_4g131030 71.329 143 24 1 18 143 1 143 1.14e-66 201
MS.gene96889.t1 MTR_1g063160 73.810 126 33 0 18 143 1 126 5.35e-63 191
MS.gene96889.t1 MTR_1g054265 69.841 126 38 0 18 143 1 126 1.10e-58 179
MS.gene96889.t1 MTR_8g051580 57.143 126 54 0 18 143 1 126 1.83e-47 151
MS.gene96889.t1 MTR_8g046350 56.667 120 49 1 18 137 1 117 1.93e-44 143
MS.gene96889.t1 MTR_4g094632 43.119 109 62 0 18 126 1 109 1.64e-25 95.1
MS.gene96889.t1 MTR_3g080940 42.149 121 68 1 18 136 8 128 1.86e-25 96.3
MS.gene96889.t1 MTR_1g077360 43.478 115 59 2 13 125 3 113 6.41e-24 93.2
MS.gene96889.t1 MTR_1g106070 42.991 107 59 1 13 117 3 109 9.06e-24 91.3
MS.gene96889.t1 MTR_3g052870 38.298 141 76 3 13 143 3 142 6.44e-23 90.1
MS.gene96889.t1 MTR_2g030740 54.667 75 34 0 13 87 3 77 6.97e-23 90.5
MS.gene96889.t1 MTR_4g094638 45.545 101 52 2 18 115 1 101 9.52e-23 88.6
MS.gene96889.t1 MTR_3g052920 53.333 75 35 0 13 87 3 77 1.22e-22 89.7
MS.gene96889.t1 MTR_1g105920 54.667 75 34 0 13 87 3 77 1.40e-22 89.7
MS.gene96889.t1 MTR_5g045560 50.667 75 37 0 13 87 3 77 1.42e-22 89.7
MS.gene96889.t1 MTR_1g075600 57.746 71 30 0 19 89 9 79 2.01e-22 89.4
MS.gene96889.t1 MTR_2g105290 39.286 112 66 1 18 129 11 120 2.93e-22 87.4
MS.gene96889.t1 MTR_5g053390 50.649 77 38 0 13 89 3 79 4.55e-22 88.2
MS.gene96889.t1 MTR_1g108510 56.522 69 30 0 19 87 9 77 5.98e-22 87.8
MS.gene96889.t1 MTR_3g030770 56.522 69 30 0 19 87 9 77 6.58e-22 87.8
MS.gene96889.t1 MTR_1g105910 53.333 75 35 0 13 87 3 77 1.29e-21 87.0
MS.gene96889.t1 MTR_3g030780 39.316 117 67 2 13 126 3 118 2.13e-21 84.0
MS.gene96889.t1 MTR_0121s0100 55.072 69 31 0 19 87 9 77 2.56e-21 83.6
MS.gene96889.t1 MTR_8g043650 55.072 69 31 0 19 87 9 77 2.83e-21 86.3
MS.gene96889.t1 MTR_1g012570 39.316 117 68 2 13 127 3 118 3.50e-21 85.9
MS.gene96889.t1 MTR_8g086290 42.342 111 57 3 19 127 12 117 3.80e-21 84.3
MS.gene96889.t1 MTR_5g055100 46.988 83 44 0 13 95 3 85 9.04e-21 84.7
MS.gene96889.t1 MTR_2g085250 53.623 69 32 0 19 87 9 77 1.05e-20 84.7
MS.gene96889.t1 MTR_2g085280 53.623 69 32 0 19 87 9 77 1.18e-20 84.7
MS.gene96889.t1 MTR_7g028448 52.308 65 31 0 33 97 26 90 1.67e-20 82.4
MS.gene96889.t1 MTR_4g036915 52.308 65 31 0 33 97 26 90 1.67e-20 82.4
MS.gene96889.t1 MTR_1g108580 52.174 69 33 0 19 87 9 77 2.43e-20 83.6
MS.gene96889.t1 MTR_2g093190 55.072 69 31 0 19 87 9 77 3.06e-20 83.2
MS.gene96889.t1 MTR_0121s0080 41.818 110 62 1 19 126 9 118 5.38e-20 82.8
MS.gene96889.t1 MTR_2g017865 41.346 104 58 1 18 121 16 116 2.30e-18 79.0
MS.gene96889.t1 MTR_7g062350 39.167 120 54 3 11 123 16 123 4.14e-18 78.2
MS.gene96889.t1 MTR_0003s0590 45.570 79 42 1 18 96 1 78 5.78e-18 77.8
MS.gene96889.t1 MTR_8g087860 44.186 86 48 0 18 103 17 102 1.35e-17 76.6
MS.gene96889.t1 MTR_1g105905 60.000 55 22 0 35 89 8 62 2.18e-17 75.5
MS.gene96889.t1 MTR_3g452380 47.500 80 38 1 11 86 5 84 4.91e-17 75.1
MS.gene96889.t1 MTR_5g066180 45.833 72 39 0 18 89 1 72 1.28e-16 73.6
MS.gene96889.t1 MTR_5g021270 46.774 62 33 0 18 79 1 62 3.37e-16 72.8
MS.gene96889.t1 MTR_5g021270 46.774 62 33 0 18 79 1 62 3.39e-16 72.8
MS.gene96889.t1 MTR_1g053070 44.928 69 38 0 18 86 1 69 5.47e-16 72.4
MS.gene96889.t1 MTR_3g005530 44.000 75 42 0 18 92 1 75 1.03e-15 71.2
MS.gene96889.t1 MTR_2g009890 36.036 111 70 1 18 128 1 110 1.79e-15 71.2
MS.gene96889.t1 MTR_8g033270 44.928 69 38 0 18 86 1 69 1.86e-15 70.9
MS.gene96889.t1 MTR_4g127140 46.250 80 43 0 18 97 11 90 7.76e-15 68.2
MS.gene96889.t1 MTR_5g066960 48.333 60 31 0 18 77 1 60 8.13e-15 65.1
MS.gene96889.t1 MTR_1g108500 63.043 46 17 0 35 80 113 158 9.64e-15 69.7
MS.gene96889.t1 MTR_1g101970 42.254 71 41 0 18 88 1 71 1.06e-14 67.8
MS.gene96889.t1 MTR_3g067875 56.250 48 21 0 35 82 8 55 1.89e-14 64.3
MS.gene96889.t1 MTR_4g093970 46.269 67 36 0 16 82 25 91 2.26e-14 67.8
MS.gene96889.t1 MTR_7g075870 35.294 102 63 1 20 121 3 101 4.47e-14 66.6
MS.gene96889.t1 MTR_8g033220 46.269 67 36 0 20 86 3 69 5.11e-14 66.6
MS.gene96889.t1 MTR_8g033220 46.269 67 36 0 20 86 3 69 5.46e-14 66.6
MS.gene96889.t1 MTR_4g102530 43.284 67 38 0 20 86 3 69 6.23e-14 66.2
MS.gene96889.t1 MTR_1g038300 45.070 71 37 1 18 86 1 71 8.00e-14 66.2
MS.gene96889.t1 MTR_5g031000 43.478 69 39 0 18 86 1 69 9.35e-14 66.2
MS.gene96889.t1 MTR_5g031000 43.478 69 39 0 18 86 1 69 1.04e-13 65.9
MS.gene96889.t1 MTR_2g461710 45.614 57 31 0 18 74 1 57 1.32e-13 62.0
MS.gene96889.t1 MTR_4g084740 47.887 71 36 1 18 87 1 71 1.53e-13 66.6
MS.gene96889.t1 MTR_5g046870 47.368 57 30 0 18 74 1 57 2.86e-13 62.4
MS.gene96889.t1 MTR_7g075870 50.000 56 28 0 20 75 3 58 3.09e-13 64.3
MS.gene96889.t1 MTR_3g084980 37.895 95 54 1 18 112 1 90 3.31e-13 64.7
MS.gene96889.t1 MTR_1g029670 35.385 65 42 0 18 82 1 65 4.51e-13 63.5
MS.gene96889.t1 MTR_7g016600 47.368 57 30 0 18 74 1 57 6.05e-13 64.3
MS.gene96889.t1 MTR_3g067910 47.273 55 29 0 35 89 6 60 6.66e-13 61.2
MS.gene96889.t1 MTR_5g032150 34.694 98 51 2 18 114 1 86 7.78e-13 63.5
MS.gene96889.t1 MTR_8g097090 47.368 57 30 0 18 74 1 57 8.64e-13 63.5
MS.gene96889.t1 MTR_5g032520 34.694 98 51 2 18 114 1 86 9.44e-13 63.5
MS.gene96889.t1 MTR_7g016630 35.556 90 52 3 18 101 1 90 1.04e-12 63.5
MS.gene96889.t1 MTR_4g109830 37.143 70 43 1 18 86 1 70 1.07e-12 63.2
MS.gene96889.t1 MTR_4g093030 49.123 57 29 0 18 74 1 57 1.28e-12 62.0
MS.gene96889.t1 MTR_5g046790 43.103 58 33 0 18 75 1 58 1.46e-12 62.8
MS.gene96889.t1 MTR_6g015975 47.368 57 30 0 18 74 1 57 1.48e-12 63.2
MS.gene96889.t1 MTR_3g113030 41.935 62 36 0 18 79 1 62 1.52e-12 62.8
MS.gene96889.t1 MTR_4g036050 43.056 72 40 1 18 88 1 72 1.62e-12 62.0
MS.gene96889.t1 MTR_4g036050 43.056 72 40 1 18 88 1 72 1.62e-12 62.4
MS.gene96889.t1 MTR_4g036050 43.056 72 40 1 18 88 1 72 1.71e-12 62.0
MS.gene96889.t1 MTR_4g036050 43.056 72 40 1 18 88 1 72 1.71e-12 61.6
MS.gene96889.t1 MTR_3g102570 34.653 101 65 1 18 117 1 101 1.80e-12 63.5
MS.gene96889.t1 MTR_4g109810 29.771 131 77 3 18 133 1 131 2.25e-12 62.4
MS.gene96889.t1 MTR_8g066260 34.737 95 48 2 18 101 1 92 2.47e-12 62.4
MS.gene96889.t1 MTR_7g075850 42.254 71 39 1 18 86 1 71 2.83e-12 62.4
MS.gene96889.t1 MTR_1g041615 61.364 44 17 0 32 75 19 62 3.94e-12 60.1
MS.gene96889.t1 MTR_3g088615 35.385 65 42 0 18 82 1 65 7.46e-12 60.1
MS.gene96889.t1 MTR_6g464720 43.103 58 33 0 18 75 1 58 3.27e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96889.t1 AT2G34440 49.550 111 54 2 18 127 1 110 1.16e-31 111
MS.gene96889.t1 AT2G24840 42.400 125 64 2 18 138 62 182 5.56e-29 107
MS.gene96889.t1 AT3G66656 44.348 115 59 1 18 127 1 115 1.50e-27 100
MS.gene96889.t1 AT1G01530 41.176 119 67 1 18 136 6 121 1.75e-22 89.7
MS.gene96889.t1 AT5G60440 40.984 122 65 2 19 136 7 125 4.24e-22 89.7
MS.gene96889.t1 AT1G65360 37.615 109 68 0 18 126 6 114 1.31e-19 81.6
MS.gene96889.t1 AT1G47760 41.000 100 47 2 18 117 1 88 2.58e-19 80.1
MS.gene96889.t1 AT1G72350 56.338 71 29 1 19 89 43 111 4.17e-19 80.1
MS.gene96889.t1 AT3G57230 43.478 92 44 2 18 108 1 85 2.55e-18 76.3
MS.gene96889.t1 AT3G57230 43.478 92 44 2 18 108 1 85 1.13e-17 76.6
MS.gene96889.t1 AT3G57230 43.478 92 44 2 18 108 1 85 1.13e-17 76.6
MS.gene96889.t1 AT5G13790 40.594 101 48 2 18 117 1 90 2.69e-17 76.3
MS.gene96889.t1 AT4G36590 51.471 68 33 0 19 86 7 74 3.59e-17 75.5
MS.gene96889.t1 AT4G09960 33.036 112 75 0 17 128 1 112 4.80e-17 75.1
MS.gene96889.t1 AT4G09960 32.743 113 76 0 16 128 9 121 4.90e-17 75.1
MS.gene96889.t1 AT5G13790 40.000 105 51 2 18 121 1 94 5.45e-17 75.5
MS.gene96889.t1 AT4G09960 33.333 111 74 0 18 128 1 111 7.44e-17 74.3
MS.gene96889.t1 AT3G04100 35.772 123 61 3 14 136 9 113 8.65e-17 73.9
MS.gene96889.t1 AT4G09960 33.333 111 74 0 18 128 1 111 8.75e-17 74.3
MS.gene96889.t1 AT3G57390 46.377 69 37 0 18 86 1 69 9.17e-17 74.3
MS.gene96889.t1 AT4G09960 33.333 111 74 0 18 128 27 137 1.46e-16 73.9
MS.gene96889.t1 AT3G57390 46.377 69 37 0 18 86 1 69 1.78e-16 73.9
MS.gene96889.t1 AT2G42830 47.368 76 40 0 18 93 16 91 1.98e-16 73.6
MS.gene96889.t1 AT2G42830 47.368 76 40 0 18 93 16 91 2.05e-16 73.6
MS.gene96889.t1 AT3G58780 38.679 106 62 1 18 123 16 118 2.21e-16 73.6
MS.gene96889.t1 AT4G09960 39.773 88 53 0 18 105 96 183 2.31e-16 74.3
MS.gene96889.t1 AT3G58780 38.679 106 62 1 18 123 22 124 2.46e-16 73.6
MS.gene96889.t1 AT4G18960 36.364 110 70 0 19 128 18 127 2.53e-16 73.6
MS.gene96889.t1 AT5G51870 35.455 110 61 2 16 121 9 112 4.52e-16 72.4
MS.gene96889.t1 AT3G58780 38.679 106 62 1 18 123 16 118 5.12e-16 72.8
MS.gene96889.t1 AT1G17310 48.148 81 35 2 34 109 63 141 5.71e-16 72.0
MS.gene96889.t1 AT5G51870 35.849 106 58 2 20 121 3 102 5.85e-16 70.9
MS.gene96889.t1 AT3G58780 43.678 87 36 1 18 91 16 102 6.34e-16 72.0
MS.gene96889.t1 AT1G29962 42.466 73 42 0 19 91 8 80 6.56e-16 71.2
MS.gene96889.t1 AT2G45650 44.928 69 38 0 18 86 1 69 6.67e-16 72.4
MS.gene96889.t1 AT2G22630 46.377 69 37 0 18 86 1 69 7.24e-16 71.6
MS.gene96889.t1 AT2G22630 46.377 69 37 0 18 86 1 69 7.24e-16 71.6
MS.gene96889.t1 AT5G51860 40.741 81 48 0 20 100 3 83 7.77e-16 71.2
MS.gene96889.t1 AT5G51870 35.849 106 58 2 20 121 3 102 7.77e-16 71.2
MS.gene96889.t1 AT1G17310 48.148 81 35 2 34 109 74 152 8.86e-16 71.6
MS.gene96889.t1 AT5G51860 40.741 81 48 0 20 100 3 83 9.51e-16 71.2
MS.gene96889.t1 AT4G22950 46.269 67 36 0 20 86 3 69 1.23e-15 70.1
MS.gene96889.t1 AT3G61120 44.928 69 38 0 18 86 1 69 1.25e-15 71.2
MS.gene96889.t1 AT5G51870 35.849 106 58 2 20 121 26 125 1.42e-15 70.5
MS.gene96889.t1 AT4G37940 46.377 69 37 0 18 86 1 69 1.43e-15 70.9
MS.gene96889.t1 AT5G51870 35.849 106 58 2 20 121 3 102 1.62e-15 70.9
MS.gene96889.t1 AT4G37940 46.377 69 37 0 18 86 1 69 1.74e-15 70.5
MS.gene96889.t1 AT1G28460 43.478 69 39 0 19 87 8 76 1.82e-15 69.7
MS.gene96889.t1 AT4G37940 35.135 111 71 1 18 128 1 110 2.10e-15 69.7
MS.gene96889.t1 AT5G65070 41.558 77 43 1 18 92 1 77 3.45e-15 68.2
MS.gene96889.t1 AT2G45660 33.962 106 67 1 20 125 3 105 3.96e-15 68.6
MS.gene96889.t1 AT4G22950 46.269 67 36 0 20 86 3 69 5.72e-15 69.3
MS.gene96889.t1 AT4G22950 46.269 67 36 0 20 86 3 69 5.72e-15 69.3
MS.gene96889.t1 AT5G65070 41.558 77 43 1 18 92 1 77 5.99e-15 68.9
MS.gene96889.t1 AT5G62165 35.849 106 57 2 20 121 3 101 6.97e-15 67.8
MS.gene96889.t1 AT5G62165 35.849 106 57 2 20 121 3 101 6.97e-15 67.8
MS.gene96889.t1 AT5G62165 35.849 106 57 2 20 121 3 101 6.97e-15 67.8
MS.gene96889.t1 AT5G62165 35.849 106 57 2 20 121 3 101 6.97e-15 67.8
MS.gene96889.t1 AT5G62165 35.849 106 57 2 20 121 3 101 6.97e-15 67.8
MS.gene96889.t1 AT5G62165 35.849 106 57 2 20 121 3 101 7.65e-15 67.8
MS.gene96889.t1 AT5G62165 35.849 106 57 2 20 121 3 101 7.65e-15 67.8
MS.gene96889.t1 AT5G62165 35.849 106 57 2 20 121 3 101 7.65e-15 67.8
MS.gene96889.t1 AT5G62165 41.463 82 44 1 20 97 3 84 1.02e-14 65.9
MS.gene96889.t1 AT2G45660 33.962 106 67 1 20 125 3 105 1.25e-14 68.2
MS.gene96889.t1 AT5G65070 41.558 77 43 1 18 92 1 77 1.52e-14 68.2
MS.gene96889.t1 AT1G77980 49.296 71 35 1 18 87 1 71 1.91e-14 68.9
MS.gene96889.t1 AT5G62165 41.463 82 44 1 20 97 3 84 2.06e-14 67.4
MS.gene96889.t1 AT5G62165 41.463 82 44 1 20 97 3 84 2.06e-14 67.4
MS.gene96889.t1 AT5G62165 41.463 82 44 1 20 97 3 84 2.08e-14 67.4
MS.gene96889.t1 AT5G62165 41.463 82 44 1 20 97 3 84 2.08e-14 67.4
MS.gene96889.t1 AT5G62165 41.463 82 44 1 20 97 3 84 2.08e-14 67.4
MS.gene96889.t1 AT5G65070 43.662 71 38 1 18 86 1 71 2.10e-14 67.8
MS.gene96889.t1 AT5G10140 39.000 100 55 3 18 112 1 99 2.32e-14 66.6
MS.gene96889.t1 AT5G65070 41.558 77 43 1 18 92 1 77 2.97e-14 67.0
MS.gene96889.t1 AT1G71692 44.444 72 39 1 18 88 1 72 3.04e-14 67.0
MS.gene96889.t1 AT3G58780 52.542 59 28 0 18 76 16 74 3.18e-14 68.2
MS.gene96889.t1 AT5G10140 42.857 77 41 2 18 92 1 76 4.20e-14 66.2
MS.gene96889.t1 AT5G10140 39.000 100 55 3 18 112 1 99 4.37e-14 66.2
MS.gene96889.t1 AT2G22540 37.113 97 50 1 18 114 1 86 4.68e-14 67.0
MS.gene96889.t1 AT2G22540 37.113 97 50 1 18 114 1 86 4.68e-14 67.0
MS.gene96889.t1 AT5G10140 42.857 77 41 2 18 92 1 76 4.80e-14 66.2
MS.gene96889.t1 AT4G24540 34.737 95 61 1 18 112 1 94 6.87e-14 66.2
MS.gene96889.t1 AT1G22130 47.887 71 36 1 18 87 1 71 6.89e-14 67.4
MS.gene96889.t1 AT4G11880 51.786 56 27 0 20 75 3 58 7.57e-14 65.5
MS.gene96889.t1 AT4G11880 51.786 56 27 0 20 75 3 58 7.57e-14 65.5
MS.gene96889.t1 AT1G24260 37.113 97 56 1 18 114 1 92 7.83e-14 66.6
MS.gene96889.t1 AT1G24260 37.113 97 56 1 18 114 1 92 8.03e-14 66.6
MS.gene96889.t1 AT2G14210 50.000 58 29 0 18 75 1 58 1.01e-13 66.2
MS.gene96889.t1 AT2G14210 50.000 58 29 0 18 75 1 58 1.15e-13 65.9
MS.gene96889.t1 AT3G54340 30.070 143 83 3 18 143 1 143 1.29e-13 65.9
MS.gene96889.t1 AT3G30260 37.168 113 63 3 18 122 1 113 1.65e-13 65.9
MS.gene96889.t1 AT4G11880 51.786 56 27 0 20 75 3 58 2.61e-13 64.7
MS.gene96889.t1 AT4G11880 51.786 56 27 0 20 75 3 58 2.61e-13 64.7
MS.gene96889.t1 AT2G03710 50.877 57 28 0 18 74 1 57 3.15e-13 63.9
MS.gene96889.t1 AT5G65080 38.961 77 45 1 18 92 8 84 3.32e-13 63.9
MS.gene96889.t1 AT5G20240 40.909 66 39 0 18 83 1 66 5.28e-13 62.8
MS.gene96889.t1 AT2G03710 50.877 57 28 0 18 74 1 57 5.42e-13 64.3
MS.gene96889.t1 AT2G22540 47.368 57 30 0 18 74 1 57 5.75e-13 63.9
MS.gene96889.t1 AT2G03710 50.877 57 28 0 18 74 1 57 5.88e-13 64.3
MS.gene96889.t1 AT5G15800 47.368 57 30 0 18 74 1 57 5.96e-13 64.3
MS.gene96889.t1 AT1G24260 36.559 93 54 1 18 110 1 88 7.38e-13 63.9
MS.gene96889.t1 AT5G15800 47.368 57 30 0 18 74 1 57 8.17e-13 63.9
MS.gene96889.t1 AT1G28450 38.356 73 45 0 19 91 8 80 8.48e-13 62.8
MS.gene96889.t1 AT5G20240 40.299 67 40 0 18 84 1 67 8.50e-13 63.2
MS.gene96889.t1 AT3G02310 47.368 57 30 0 18 74 1 57 9.24e-13 63.5
MS.gene96889.t1 AT5G23260 54.386 57 26 0 18 74 17 73 9.90e-13 63.9
MS.gene96889.t1 AT3G02310 47.368 57 30 0 18 74 1 57 1.00e-12 62.8
MS.gene96889.t1 AT5G23260 54.386 57 26 0 18 74 1 57 1.05e-12 63.5
MS.gene96889.t1 AT5G23260 54.386 57 26 0 18 74 17 73 1.09e-12 63.5
MS.gene96889.t1 AT5G23260 54.386 57 26 0 18 74 1 57 1.15e-12 63.2
MS.gene96889.t1 AT1G26310 35.870 92 53 2 18 103 1 92 1.44e-12 63.2
MS.gene96889.t1 AT1G77950 37.815 119 60 5 18 123 1 118 2.21e-12 62.8
MS.gene96889.t1 AT1G77950 37.815 119 60 5 18 123 1 118 2.21e-12 62.8
MS.gene96889.t1 AT1G77950 37.815 119 60 5 18 123 1 118 2.21e-12 62.8
MS.gene96889.t1 AT1G77950 37.815 119 60 5 18 123 1 118 2.21e-12 62.8
MS.gene96889.t1 AT1G69120 44.828 58 32 0 18 75 1 58 2.86e-12 62.4
MS.gene96889.t1 AT1G46408 45.205 73 34 2 20 87 5 76 3.20e-12 62.4
MS.gene96889.t1 AT5G65060 50.909 55 27 0 18 72 1 55 3.39e-12 61.2
MS.gene96889.t1 AT5G65060 50.909 55 27 0 18 72 1 55 3.43e-12 61.2
MS.gene96889.t1 AT5G60910 43.860 57 32 0 18 74 1 57 3.47e-12 62.0
MS.gene96889.t1 AT5G65050 49.091 55 28 0 18 72 1 55 3.66e-12 60.8
MS.gene96889.t1 AT5G65060 50.909 55 27 0 18 72 1 55 3.69e-12 61.2
MS.gene96889.t1 AT5G65080 40.580 69 40 1 18 86 8 75 4.30e-12 61.2
MS.gene96889.t1 AT1G69120 44.828 58 32 0 18 75 1 58 4.98e-12 61.6
MS.gene96889.t1 AT5G65050 49.091 55 28 0 18 72 1 55 5.48e-12 60.8
MS.gene96889.t1 AT5G65050 49.091 55 28 0 18 72 1 55 5.70e-12 60.8
MS.gene96889.t1 AT5G65050 49.091 55 28 0 18 72 1 55 5.81e-12 60.5
MS.gene96889.t1 AT1G18750 39.726 73 42 1 18 88 1 73 1.72e-11 60.8
MS.gene96889.t1 AT1G18750 39.726 73 42 1 18 88 1 73 1.96e-11 60.8
MS.gene96889.t1 AT1G18750 39.726 73 42 1 18 88 1 73 2.03e-11 60.8
MS.gene96889.t1 AT5G04640 39.474 76 40 2 20 90 5 79 3.80e-11 59.7

Find 25 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGACAGACCTTACTCTTT+TGG 0.325068 1.4:+32408717 MS.gene96889:CDS
CGCTTGCATCAGTCTCTTGT+TGG 0.342002 1.4:-32408776 None:intergenic
CATTAGAAGATGGGTTTCCT+TGG 0.387460 1.4:-32408803 None:intergenic
CCACTTGTTCATTCCAGCTT+TGG 0.389651 1.4:-32408936 None:intergenic
GATTTCTGCTCCGCACAAAA+TGG 0.391049 1.4:-32408673 None:intergenic
AGTCTCTTGTTGGAGATACT+TGG 0.406802 1.4:-32408766 None:intergenic
AATGTAACTTGCCTCGTGTC+GGG 0.455568 1.4:-32408606 None:intergenic
ATTAGAAGATGGGTTTCCTT+GGG 0.457016 1.4:-32408802 None:intergenic
GCAAAATGATGTGCCAAAGC+TGG 0.459253 1.4:+32408923 MS.gene96889:CDS
ATCTTGGGAGCATTAGAAGA+TGG 0.462018 1.4:-32408813 None:intergenic
TAGTAGGTGTTGCATGAAGC+AGG 0.465801 1.4:+32408534 MS.gene96889:intron
AAAGAGTAAGGTCTGTCAAC+GGG 0.468872 1.4:-32408714 None:intergenic
AAATGACATGAGATATAAGT+TGG 0.485750 1.4:+32408128 None:intergenic
GAAGCAGGTGAAGGAATCAT+TGG 0.491417 1.4:+32408549 MS.gene96889:CDS
ATCTTCTAATGCTCCCAAGA+TGG 0.515526 1.4:+32408815 MS.gene96889:CDS
TCTTGGGAGCATTAGAAGAT+GGG 0.519973 1.4:-32408812 None:intergenic
GTTGCAACAACGTCAACACC+TGG 0.537919 1.4:-32408744 None:intergenic
CCTGGATGACCAAAAGAGTA+AGG 0.538014 1.4:-32408726 None:intergenic
AATTCGTGAAGGTGAAAAGC+AGG 0.574955 1.4:+32408875 MS.gene96889:CDS
GAATGTAACTTGCCTCGTGT+CGG 0.577167 1.4:-32408607 None:intergenic
AAAAGAGTAAGGTCTGTCAA+CGG 0.600863 1.4:-32408715 None:intergenic
GTGTTGCATGAAGCAGGTGA+AGG 0.616168 1.4:+32408540 MS.gene96889:CDS
TCGTGAAAGATCCCGACACG+AGG 0.619515 1.4:+32408595 MS.gene96889:CDS
ACTGATGCAAGCGATTCCCA+AGG 0.659023 1.4:+32408786 MS.gene96889:CDS
ATACTGACACAAATTCGTGA+AGG 0.686922 1.4:+32408864 MS.gene96889:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATCACATTTTTCAATTA+TGG - chr1.4:32408401-32408420 None:intergenic 15.0%
!! AGTAATATGATACATATAGA+AGG - chr1.4:32408183-32408202 None:intergenic 20.0%
!! ATTACAATCCCAAAATATTT+AGG + chr1.4:32408317-32408336 MS.gene96889:intron 20.0%
!! CTAGAATGTATACATAAAAA+GGG + chr1.4:32408448-32408467 MS.gene96889:intron 20.0%
!! TAGAATGTATACATAAAAAG+GGG + chr1.4:32408449-32408468 MS.gene96889:intron 20.0%
!! TTACAATCCCAAAATATTTA+GGG + chr1.4:32408318-32408337 MS.gene96889:intron 20.0%
!!! AGATTTAATTTTTCCATCTT+GGG - chr1.4:32408831-32408850 None:intergenic 20.0%
!!! TAGATTTAATTTTTCCATCT+TGG - chr1.4:32408832-32408851 None:intergenic 20.0%
!!! TCTAGAATGTATACATAAAA+AGG + chr1.4:32408447-32408466 MS.gene96889:intron 20.0%
! AACACCTACTAAAAAAAAGA+AGG - chr1.4:32408525-32408544 None:intergenic 25.0%
! ATAACTAGAAAAGTAGACAT+TGG - chr1.4:32408257-32408276 None:intergenic 25.0%
! TAACTAGAAAAGTAGACATT+GGG - chr1.4:32408256-32408275 None:intergenic 25.0%
! TACAATCCCAAAATATTTAG+GGG + chr1.4:32408319-32408338 MS.gene96889:intron 25.0%
!! AAAATTGCCCCTAAATATTT+TGG - chr1.4:32408329-32408348 None:intergenic 25.0%
!! AAATTGCCCCTAAATATTTT+GGG - chr1.4:32408328-32408347 None:intergenic 25.0%
AAAGTAGACATTGGGTTTAT+TGG - chr1.4:32408248-32408267 None:intergenic 30.0%
! AATGAATTGTCCATTTTGTG+CGG + chr1.4:32408663-32408682 MS.gene96889:CDS 30.0%
!!! CTTGCCTTCTTTTTTTTAGT+AGG + chr1.4:32408518-32408537 MS.gene96889:intron 30.0%
AAAAGAGTAAGGTCTGTCAA+CGG - chr1.4:32408718-32408737 None:intergenic 35.0%
AGAAGGACTTTATTGCGAAT+CGG - chr1.4:32408166-32408185 None:intergenic 35.0%
ATACTGACACAAATTCGTGA+AGG + chr1.4:32408864-32408883 MS.gene96889:CDS 35.0%
! ATTAGAAGATGGGTTTCCTT+GGG - chr1.4:32408805-32408824 None:intergenic 35.0%
AAAGAGTAAGGTCTGTCAAC+GGG - chr1.4:32408717-32408736 None:intergenic 40.0%
AATTCGTGAAGGTGAAAAGC+AGG + chr1.4:32408875-32408894 MS.gene96889:CDS 40.0%
AGTCTCTTGTTGGAGATACT+TGG - chr1.4:32408769-32408788 None:intergenic 40.0%
ATCTTCTAATGCTCCCAAGA+TGG + chr1.4:32408815-32408834 MS.gene96889:CDS 40.0%
! CATTAGAAGATGGGTTTCCT+TGG - chr1.4:32408806-32408825 None:intergenic 40.0%
! GTTGACAGACCTTACTCTTT+TGG + chr1.4:32408717-32408736 MS.gene96889:CDS 40.0%
!! ATCTTGGGAGCATTAGAAGA+TGG - chr1.4:32408816-32408835 None:intergenic 40.0%
!! TCTTGGGAGCATTAGAAGAT+GGG - chr1.4:32408815-32408834 None:intergenic 40.0%
CCTGGATGACCAAAAGAGTA+AGG - chr1.4:32408729-32408748 None:intergenic 45.0%
GAAGCAGGTGAAGGAATCAT+TGG + chr1.4:32408549-32408568 MS.gene96889:CDS 45.0%
GATTTCTGCTCCGCACAAAA+TGG - chr1.4:32408676-32408695 None:intergenic 45.0%
GCAAAATGATGTGCCAAAGC+TGG + chr1.4:32408923-32408942 MS.gene96889:CDS 45.0%
! AATGTAACTTGCCTCGTGTC+GGG - chr1.4:32408609-32408628 None:intergenic 45.0%
! CCTTACTCTTTTGGTCATCC+AGG + chr1.4:32408726-32408745 MS.gene96889:CDS 45.0%
! GAATGTAACTTGCCTCGTGT+CGG - chr1.4:32408610-32408629 None:intergenic 45.0%
! TAGTAGGTGTTGCATGAAGC+AGG + chr1.4:32408534-32408553 MS.gene96889:intron 45.0%
ACTGATGCAAGCGATTCCCA+AGG + chr1.4:32408786-32408805 MS.gene96889:CDS 50.0%
CGCTTGCATCAGTCTCTTGT+TGG - chr1.4:32408779-32408798 None:intergenic 50.0%
GTGTTGCATGAAGCAGGTGA+AGG + chr1.4:32408540-32408559 MS.gene96889:CDS 50.0%
GTTGCAACAACGTCAACACC+TGG - chr1.4:32408747-32408766 None:intergenic 50.0%
TCGTGAAAGATCCCGACACG+AGG + chr1.4:32408595-32408614 MS.gene96889:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 32408135 32408950 32408135 ID=MS.gene96889
chr1.4 mRNA 32408135 32408950 32408135 ID=MS.gene96889.t1;Parent=MS.gene96889
chr1.4 exon 32408135 32408158 32408135 ID=MS.gene96889.t1.exon1;Parent=MS.gene96889.t1
chr1.4 CDS 32408135 32408158 32408135 ID=cds.MS.gene96889.t1;Parent=MS.gene96889.t1
chr1.4 exon 32408540 32408950 32408540 ID=MS.gene96889.t1.exon2;Parent=MS.gene96889.t1
chr1.4 CDS 32408540 32408950 32408540 ID=cds.MS.gene96889.t1;Parent=MS.gene96889.t1
Gene Sequence

>MS.gene96889

ATGAGATATAAGTTGGCGCTAGCTGTGACCGATTCGCAATAAAGTCCTTCTATATGTATCATATTACTATAATAATATCAAATAAAGATAAGATTTGTATTACTTGTTTTCCAATAAACCCAATGTCTACTTTTCTAGTTATCAGTACAATTGAATTGAATTAAAAATAAATAAAATATTAAATTACAATCCCAAAATATTTAGGGGCAATTTTGTAAAATTATAATGATAATAAAATATTTCTATGTTAGTGATAATAATGTCCATAATTGAAAAATGTGATATACACAATTGAAAATGTAATAAAATATTTCTAGAATGTATACATAAAAAGGGGAGTAAATTATACACATAACATTTATAATCAATCTTTGTGTTCTAAACTTGCCTTCTTTTTTTTAGTAGGTGTTGCATGAAGCAGGTGAAGGAATCATTGGTCGTCGTAAAATTGAGATTGAGATCGTGAAAGATCCCGACACGAGGCAAGTTACATTCTCAAAGCGTCGAACGAGCTTATTCAAGAAAGCAAATGAATTGTCCATTTTGTGCGGAGCAGAAATCGCTACTGTTGTGTTCTCTCCCGTTGACAGACCTTACTCTTTTGGTCATCCAGGTGTTGACGTTGTTGCAACCAAGTATCTCCAACAAGAGACTGATGCAAGCGATTCCCAAGGAAACCCATCTTCTAATGCTCCCAAGATGGAAAAATTAAATCTAGAACTTGATGAGATACTGACACAAATTCGTGAAGGTGAAAAGCAGGTCGAGACTCATGATGAGATTCTAAAGCAAAATGATGTGCCAAAGCTGGAATGA
Protein sequence

>MS.gene96889.t1

MRYKLALAVLHEAGEGIIGRRKIEIEIVKDPDTRQVTFSKRRTSLFKKANELSILCGAEIATVVFSPVDRPYSFGHPGVDVVATKYLQQETDASDSQGNPSSNAPKMEKLNLELDEILTQIREGEKQVETHDEILKQNDVPKLE