AlfalfaGEDB | MS.gene96889

M. sativa cultivar XinJiangDaYe / MS.gene96889

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96889.t1	XP_013467229.1	88.1	126	15	0	18	143	1	126	9.80E-54	219.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96889.t1	O64703	49.5	111	54	2	18	127	1	110	8.4e-22	104.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96889.t1	A0A072VIJ3	88.1	126	15	0	18	143	1	126	7.0e-54	219.5

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene96889.t1	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96889.t1	MTR_1g047550	88.095	126	15	0	18	143	1	126	6.51e-80	234
MS.gene96889.t1	MTR_8g079502	83.333	126	21	0	18	143	1	126	3.26e-74	219
MS.gene96889.t1	MTR_4g131030	71.329	143	24	1	18	143	1	143	1.14e-66	201
MS.gene96889.t1	MTR_1g063160	73.810	126	33	0	18	143	1	126	5.35e-63	191
MS.gene96889.t1	MTR_1g054265	69.841	126	38	0	18	143	1	126	1.10e-58	179
MS.gene96889.t1	MTR_8g051580	57.143	126	54	0	18	143	1	126	1.83e-47	151
MS.gene96889.t1	MTR_8g046350	56.667	120	49	1	18	137	1	117	1.93e-44	143
MS.gene96889.t1	MTR_4g094632	43.119	109	62	0	18	126	1	109	1.64e-25	95.1
MS.gene96889.t1	MTR_3g080940	42.149	121	68	1	18	136	8	128	1.86e-25	96.3
MS.gene96889.t1	MTR_1g077360	43.478	115	59	2	13	125	3	113	6.41e-24	93.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96889.t1	AT2G34440	49.550	111	54	2	18	127	1	110	1.16e-31	111
MS.gene96889.t1	AT2G24840	42.400	125	64	2	18	138	62	182	5.56e-29	107
MS.gene96889.t1	AT3G66656	44.348	115	59	1	18	127	1	115	1.50e-27	100
MS.gene96889.t1	AT1G01530	41.176	119	67	1	18	136	6	121	1.75e-22	89.7
MS.gene96889.t1	AT5G60440	40.984	122	65	2	19	136	7	125	4.24e-22	89.7
MS.gene96889.t1	AT1G65360	37.615	109	68	0	18	126	6	114	1.31e-19	81.6
MS.gene96889.t1	AT1G47760	41.000	100	47	2	18	117	1	88	2.58e-19	80.1
MS.gene96889.t1	AT1G72350	56.338	71	29	1	19	89	43	111	4.17e-19	80.1
MS.gene96889.t1	AT3G57230	43.478	92	44	2	18	108	1	85	2.55e-18	76.3
MS.gene96889.t1	AT3G57230	43.478	92	44	2	18	108	1	85	1.13e-17	76.6

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Find 25 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGTAAGGTCTGTCAA+CGG	0.600863	1.4:-32408715	None:intergenic
AAAGAGTAAGGTCTGTCAAC+GGG	0.468872	1.4:-32408714	None:intergenic
AAATGACATGAGATATAAGT+TGG	0.485750	1.4:+32408128	None:intergenic
AATGTAACTTGCCTCGTGTC+GGG	0.455568	1.4:-32408606	None:intergenic
AATTCGTGAAGGTGAAAAGC+AGG	0.574955	1.4:+32408875	MS.gene96889:CDS
ACTGATGCAAGCGATTCCCA+AGG	0.659023	1.4:+32408786	MS.gene96889:CDS
AGTCTCTTGTTGGAGATACT+TGG	0.406802	1.4:-32408766	None:intergenic
ATACTGACACAAATTCGTGA+AGG	0.686922	1.4:+32408864	MS.gene96889:CDS
ATCTTCTAATGCTCCCAAGA+TGG	0.515526	1.4:+32408815	MS.gene96889:CDS
ATCTTGGGAGCATTAGAAGA+TGG	0.462018	1.4:-32408813	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGTAGACATTGGGTTTAT+TGG	-	chr1.4:32408248-32408267	None:intergenic	30.0%
AAAAGAGTAAGGTCTGTCAA+CGG	-	chr1.4:32408718-32408737	None:intergenic	35.0%
AGAAGGACTTTATTGCGAAT+CGG	-	chr1.4:32408166-32408185	None:intergenic	35.0%
ATACTGACACAAATTCGTGA+AGG	+	chr1.4:32408864-32408883	MS.gene96889:CDS	35.0%
AAAGAGTAAGGTCTGTCAAC+GGG	-	chr1.4:32408717-32408736	None:intergenic	40.0%
AATTCGTGAAGGTGAAAAGC+AGG	+	chr1.4:32408875-32408894	MS.gene96889:CDS	40.0%
AGTCTCTTGTTGGAGATACT+TGG	-	chr1.4:32408769-32408788	None:intergenic	40.0%
ATCTTCTAATGCTCCCAAGA+TGG	+	chr1.4:32408815-32408834	MS.gene96889:CDS	40.0%
CCTGGATGACCAAAAGAGTA+AGG	-	chr1.4:32408729-32408748	None:intergenic	45.0%
GAAGCAGGTGAAGGAATCAT+TGG	+	chr1.4:32408549-32408568	MS.gene96889:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	32408135	32408950	32408135	ID=MS.gene96889
chr1.4	mRNA	32408135	32408950	32408135	ID=MS.gene96889.t1;Parent=MS.gene96889
chr1.4	exon	32408135	32408158	32408135	ID=MS.gene96889.t1.exon1;Parent=MS.gene96889.t1
chr1.4	CDS	32408135	32408158	32408135	ID=cds.MS.gene96889.t1;Parent=MS.gene96889.t1
chr1.4	exon	32408540	32408950	32408540	ID=MS.gene96889.t1.exon2;Parent=MS.gene96889.t1
chr1.4	CDS	32408540	32408950	32408540	ID=cds.MS.gene96889.t1;Parent=MS.gene96889.t1

Gene Sequence

>MS.gene96889

ATGAGATATAAGTTGGCGCTAGCTGTGACCGATTCGCAATAAAGTCCTTCTATATGTATCATATTACTATAATAATATCAAATAAAGATAAGATTTGTATTACTTGTTTTCCAATAAACCCAATGTCTACTTTTCTAGTTATCAGTACAATTGAATTGAATTAAAAATAAATAAAATATTAAATTACAATCCCAAAATATTTAGGGGCAATTTTGTAAAATTATAATGATAATAAAATATTTCTATGTTAGTGATAATAATGTCCATAATTGAAAAATGTGATATACACAATTGAAAATGTAATAAAATATTTCTAGAATGTATACATAAAAAGGGGAGTAAATTATACACATAACATTTATAATCAATCTTTGTGTTCTAAACTTGCCTTCTTTTTTTTAGTAGGTGTTGCATGAAGCAGGTGAAGGAATCATTGGTCGTCGTAAAATTGAGATTGAGATCGTGAAAGATCCCGACACGAGGCAAGTTACATTCTCAAAGCGTCGAACGAGCTTATTCAAGAAAGCAAATGAATTGTCCATTTTGTGCGGAGCAGAAATCGCTACTGTTGTGTTCTCTCCCGTTGACAGACCTTACTCTTTTGGTCATCCAGGTGTTGACGTTGTTGCAACCAAGTATCTCCAACAAGAGACTGATGCAAGCGATTCCCAAGGAAACCCATCTTCTAATGCTCCCAAGATGGAAAAATTAAATCTAGAACTTGATGAGATACTGACACAAATTCGTGAAGGTGAAAAGCAGGTCGAGACTCATGATGAGATTCTAAAGCAAAATGATGTGCCAAAGCTGGAATGA

Protein sequence

>MS.gene96889.t1

MRYKLALAVLHEAGEGIIGRRKIEIEIVKDPDTRQVTFSKRRTSLFKKANELSILCGAEIATVVFSPVDRPYSFGHPGVDVVATKYLQQETDASDSQGNPSSNAPKMEKLNLELDEILTQIREGEKQVETHDEILKQNDVPKLE