Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97373.t1 | AES63738.2 | 84.4 | 122 | 19 | 0 | 1 | 122 | 1 | 122 | 7.50E-55 | 223 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97373.t1 | G7INX6 | 84.4 | 122 | 19 | 0 | 1 | 122 | 1 | 122 | 5.4e-55 | 223.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene97373.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97373.t1 | MTR_2g012700 | 84.426 | 122 | 19 | 0 | 1 | 122 | 1 | 122 | 1.75e-74 | 217 |
| MS.gene97373.t1 | MTR_2g062330 | 80.833 | 120 | 22 | 1 | 3 | 122 | 23 | 141 | 1.72e-67 | 200 |
| MS.gene97373.t1 | MTR_7g117590 | 35.385 | 130 | 65 | 4 | 7 | 119 | 44 | 171 | 8.03e-21 | 82.8 |
| MS.gene97373.t1 | MTR_7g117585 | 35.644 | 101 | 63 | 1 | 18 | 116 | 42 | 142 | 2.26e-17 | 73.2 |
| MS.gene97373.t1 | MTR_0010s0310 | 40.659 | 91 | 52 | 1 | 22 | 110 | 150 | 240 | 5.72e-17 | 74.3 |
| MS.gene97373.t1 | MTR_3g008190 | 28.866 | 97 | 69 | 0 | 24 | 120 | 6 | 102 | 6.56e-16 | 68.2 |
| MS.gene97373.t1 | MTR_5g071410 | 33.663 | 101 | 65 | 1 | 18 | 116 | 165 | 265 | 1.45e-14 | 68.2 |
| MS.gene97373.t1 | MTR_6g477950 | 35.802 | 81 | 52 | 0 | 39 | 119 | 64 | 144 | 4.70e-13 | 64.3 |
| MS.gene97373.t1 | MTR_6g087720 | 33.721 | 86 | 56 | 1 | 24 | 108 | 214 | 299 | 1.20e-12 | 63.2 |
| MS.gene97373.t1 | MTR_0010s0320 | 36.264 | 91 | 54 | 2 | 24 | 110 | 192 | 282 | 3.30e-12 | 61.6 |
| MS.gene97373.t1 | MTR_0010s0290 | 34.021 | 97 | 60 | 2 | 18 | 110 | 217 | 313 | 5.87e-12 | 61.2 |
| MS.gene97373.t1 | MTR_5g078270 | 32.967 | 91 | 59 | 1 | 22 | 110 | 208 | 298 | 9.41e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTACTGCTTCTATTT+TGG | 0.123735 | 3.1:+10625267 | MS.gene97373:CDS |
| AAAATTCCGATGTTCATTAT+TGG | 0.199581 | 3.1:+10625239 | MS.gene97373:CDS |
| GAAGTCAATGGTGCTGAATT+AGG | 0.200131 | 3.1:+10625208 | MS.gene97373:CDS |
| TACCAGTTTCTTCATCAAAA+AGG | 0.252196 | 3.1:-10625458 | None:intergenic |
| TGTATGCTACCGTCGTTAAC+TGG | 0.297739 | 3.1:+10625625 | MS.gene97373:CDS |
| CCTCGACGTGTTGCCGAGAA+TGG | 0.307864 | 3.1:+10625408 | MS.gene97373:CDS |
| CGGTCAATAATCCTCCTAAT+AGG | 0.355854 | 3.1:+10625601 | MS.gene97373:CDS |
| TTTGGATGGAAACAATGTTT+TGG | 0.356448 | 3.1:+10625285 | MS.gene97373:CDS |
| AAGTCAATGGTGCTGAATTA+GGG | 0.416476 | 3.1:+10625209 | MS.gene97373:CDS |
| ACGGTAGCATACATCCTATT+AGG | 0.440754 | 3.1:-10625615 | None:intergenic |
| ATTGGTATACAAACCATTCT+CGG | 0.470714 | 3.1:-10625421 | None:intergenic |
| GTATGCTACCGTCGTTAACT+GGG | 0.522932 | 3.1:+10625626 | MS.gene97373:CDS |
| GCATGGATCAGAAATACATA+GGG | 0.559318 | 3.1:+10625517 | MS.gene97373:CDS |
| AGCATGGATCAGAAATACAT+AGG | 0.564126 | 3.1:+10625516 | MS.gene97373:CDS |
| CTTGAAAAGTGCCTAAAAGT+TGG | 0.570138 | 3.1:+10625561 | MS.gene97373:CDS |
| AGAAATACATAGGGAGAGGA+TGG | 0.573439 | 3.1:+10625526 | MS.gene97373:CDS |
| TCACGCTGCGTCAAGAAGCA+TGG | 0.586224 | 3.1:+10625500 | MS.gene97373:CDS |
| GATCAGAAATACATAGGGAG+AGG | 0.587262 | 3.1:+10625522 | MS.gene97373:CDS |
| CCATTCTCGGCAACACGTCG+AGG | 0.599354 | 3.1:-10625408 | None:intergenic |
| GAGATTGGAGTTGAAGTCAA+TGG | 0.619642 | 3.1:+10625196 | MS.gene97373:CDS |
| GTAGCATACATCCTATTAGG+AGG | 0.633251 | 3.1:-10625612 | None:intergenic |
| ATACTTCCAATAATGAACAT+CGG | 0.646998 | 3.1:-10625245 | None:intergenic |
| TAATCAGTCCCAGTTAACGA+CGG | 0.733495 | 3.1:-10625634 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCCGATGTTCATTAT+TGG | + | chr3.1:10625239-10625258 | MS.gene97373:CDS | 25.0% |
| ! | ATACTTCCAATAATGAACAT+CGG | - | chr3.1:10625248-10625267 | None:intergenic | 25.0% |
| !! | AAGGAGAATTTTAGATTGAT+TGG | - | chr3.1:10625442-10625461 | None:intergenic | 25.0% |
| !! | TAATGTTACTGCTTCTATTT+TGG | + | chr3.1:10625267-10625286 | MS.gene97373:CDS | 25.0% |
| ATTGGTATACAAACCATTCT+CGG | - | chr3.1:10625424-10625443 | None:intergenic | 30.0% | |
| TACCAGTTTCTTCATCAAAA+AGG | - | chr3.1:10625461-10625480 | None:intergenic | 30.0% | |
| ! | TTTGGATGGAAACAATGTTT+TGG | + | chr3.1:10625285-10625304 | MS.gene97373:CDS | 30.0% |
| AGCATGGATCAGAAATACAT+AGG | + | chr3.1:10625516-10625535 | MS.gene97373:CDS | 35.0% | |
| GCATGGATCAGAAATACATA+GGG | + | chr3.1:10625517-10625536 | MS.gene97373:CDS | 35.0% | |
| ! | AAACAACGTCACCAACTTTT+AGG | - | chr3.1:10625575-10625594 | None:intergenic | 35.0% |
| ! | AAGTCAATGGTGCTGAATTA+GGG | + | chr3.1:10625209-10625228 | MS.gene97373:CDS | 35.0% |
| ! | CTCCTTTTTGATGAAGAAAC+TGG | + | chr3.1:10625456-10625475 | MS.gene97373:CDS | 35.0% |
| !! | CTTGAAAAGTGCCTAAAAGT+TGG | + | chr3.1:10625561-10625580 | MS.gene97373:CDS | 35.0% |
| !! | GTTACTGCTTCTATTTTGGA+TGG | + | chr3.1:10625271-10625290 | MS.gene97373:CDS | 35.0% |
| !!! | GGAAACAATGTTTTGGTTAG+TGG | + | chr3.1:10625292-10625311 | MS.gene97373:intron | 35.0% |
| ACGGTAGCATACATCCTATT+AGG | - | chr3.1:10625618-10625637 | None:intergenic | 40.0% | |
| AGAAATACATAGGGAGAGGA+TGG | + | chr3.1:10625526-10625545 | MS.gene97373:CDS | 40.0% | |
| CGGTCAATAATCCTCCTAAT+AGG | + | chr3.1:10625601-10625620 | MS.gene97373:CDS | 40.0% | |
| GAGATTGGAGTTGAAGTCAA+TGG | + | chr3.1:10625196-10625215 | MS.gene97373:CDS | 40.0% | |
| GATCAGAAATACATAGGGAG+AGG | + | chr3.1:10625522-10625541 | MS.gene97373:CDS | 40.0% | |
| GTAGCATACATCCTATTAGG+AGG | - | chr3.1:10625615-10625634 | None:intergenic | 40.0% | |
| ! | GAAGTCAATGGTGCTGAATT+AGG | + | chr3.1:10625208-10625227 | MS.gene97373:CDS | 40.0% |
| !! | TGGTGACGTTGTTTTGTTCA+CGG | + | chr3.1:10625581-10625600 | MS.gene97373:CDS | 40.0% |
| GTATGCTACCGTCGTTAACT+GGG | + | chr3.1:10625626-10625645 | MS.gene97373:CDS | 45.0% | |
| TGTATGCTACCGTCGTTAAC+TGG | + | chr3.1:10625625-10625644 | MS.gene97373:CDS | 45.0% | |
| TCACGCTGCGTCAAGAAGCA+TGG | + | chr3.1:10625500-10625519 | MS.gene97373:CDS | 55.0% | |
| CCATTCTCGGCAACACGTCG+AGG | - | chr3.1:10625411-10625430 | None:intergenic | 60.0% | |
| CCTCGACGTGTTGCCGAGAA+TGG | + | chr3.1:10625408-10625427 | MS.gene97373:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 10625190 | 10625653 | 10625190 | ID=MS.gene97373 |
| chr3.1 | mRNA | 10625190 | 10625653 | 10625190 | ID=MS.gene97373.t1;Parent=MS.gene97373 |
| chr3.1 | exon | 10625190 | 10625306 | 10625190 | ID=MS.gene97373.t1.exon1;Parent=MS.gene97373.t1 |
| chr3.1 | CDS | 10625190 | 10625306 | 10625190 | ID=cds.MS.gene97373.t1;Parent=MS.gene97373.t1 |
| chr3.1 | exon | 10625402 | 10625653 | 10625402 | ID=MS.gene97373.t1.exon2;Parent=MS.gene97373.t1 |
| chr3.1 | CDS | 10625402 | 10625653 | 10625402 | ID=cds.MS.gene97373.t1;Parent=MS.gene97373.t1 |
| Gene Sequence |
| Protein sequence |
