AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97637

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97637.t1 MTR_1g053575 66.163 331 41 5 1 268 1 323 3.71e-139 394
MS.gene97637.t1 MTR_1g045470 51.515 330 91 8 1 267 1 324 2.46e-91 273
MS.gene97637.t1 MTR_7g116460 59.615 208 51 6 1 183 1 200 1.27e-76 231
MS.gene97637.t1 MTR_1g019820 34.783 207 80 4 102 254 5 210 1.99e-26 103
MS.gene97637.t1 MTR_7g005280 32.738 168 90 9 11 170 19 171 3.53e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97637.t1 AT5G13180 34.194 155 79 10 11 158 18 156 7.68e-11 61.2

Find 52 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGAATCCTTGGGATATT+TGG 0.040443 1.1:-39983949 MS.gene97637:CDS
GTTTCATGAAGAGTTATTAT+TGG 0.203680 1.1:-39982220 MS.gene97637:CDS
TTCAAGAATGGTGATGTAAT+TGG 0.213249 1.1:+39983991 None:intergenic
AAATATCCCAAGGATTCTTT+AGG 0.262491 1.1:+39983951 None:intergenic
TCATTGTCAGGTTGAACTAT+TGG 0.266404 1.1:+39982417 None:intergenic
CAGGTTGAACTATTGGATTT+TGG 0.311938 1.1:+39982424 None:intergenic
AAAGAATCCTTGGGATATTT+GGG 0.338135 1.1:-39983948 MS.gene97637:CDS
TGGGATATTTGGGAAGAGTT+TGG 0.341365 1.1:-39983938 MS.gene97637:CDS
ATTCTTGAATACGATTTGTT+TGG 0.353129 1.1:-39983977 MS.gene97637:CDS
ATCTTTGATAAGAAATCCTT+TGG 0.365896 1.1:-39983663 MS.gene97637:intron
TCTCAATTCTTCTTCAAGTA+AGG 0.366602 1.1:-39984086 MS.gene97637:CDS
TTAAGGTAGTGAAGAAATAT+AGG 0.367031 1.1:+39983888 None:intergenic
AATTGGGAAGAGATTTGTTA+TGG 0.367059 1.1:+39984008 None:intergenic
TCGAAAGTAGTTCGTTCTAT+TGG 0.368294 1.1:-39983839 MS.gene97637:CDS
AATATCCCAAGGATTCTTTA+GGG 0.371252 1.1:+39983952 None:intergenic
CAGTGGTAGATTTCTTCTCT+AGG 0.372963 1.1:+39983864 None:intergenic
GTAGTTCGTTCTATTGGAAT+TGG 0.374249 1.1:-39983833 MS.gene97637:CDS
TCAAGAATGGTGATGTAATT+GGG 0.404508 1.1:+39983992 None:intergenic
AGATTTCTTCTCTAGGGTTA+AGG 0.417289 1.1:+39983871 None:intergenic
GAAAGAAAGACCATTGTTCC+TGG 0.417462 1.1:-39983491 MS.gene97637:CDS
TCATCATCATCTTCATTGTC+AGG 0.417702 1.1:+39982405 None:intergenic
AAATGACGACGAAAGCTTTG+TGG 0.419890 1.1:-39982358 MS.gene97637:CDS
TTGGTACCCTAAAGAATCCT+TGG 0.424102 1.1:-39983958 MS.gene97637:CDS
AATCAAGTAACTTACAGATA+TGG 0.426262 1.1:+39983454 None:intergenic
TCTGCATAGAACATAATTGT+TGG 0.465415 1.1:+39983544 None:intergenic
TGGGAAGGTGAAGAAGATAC+TGG 0.487089 1.1:-39983806 MS.gene97637:CDS
TGGTACCCTAAAGAATCCTT+GGG 0.489046 1.1:-39983957 MS.gene97637:CDS
AGTGGTAGATTTCTTCTCTA+GGG 0.491270 1.1:+39983865 None:intergenic
GTTCTATTGGAATTGGTGCA+TGG 0.508402 1.1:-39983826 MS.gene97637:CDS
AAACAAATCGTATTCAAGAA+TGG 0.519788 1.1:+39983979 None:intergenic
GCACTTACCATGATGGTGTA+TGG 0.524546 1.1:-39983712 MS.gene97637:CDS
GACAATGAAGATGATGATGA+TGG 0.525335 1.1:-39982402 MS.gene97637:CDS
ATGCTTGATCGAAGAGGAAG+AGG 0.530574 1.1:-39982262 MS.gene97637:CDS
GAGCTGATAGAGATGAATGA+AGG 0.539230 1.1:-39982285 MS.gene97637:CDS
ATGGTGGTTTCTGCACGTCC+AGG 0.545124 1.1:+39983473 None:intergenic
TTCTATTGGAATTGGTGCAT+GGG 0.546914 1.1:-39983825 MS.gene97637:CDS
TTCTTATCAAAGATTTGTCG+AGG 0.569506 1.1:+39983672 None:intergenic
ATGATGATGCTGGTATGTCT+TGG 0.577942 1.1:-39982326 MS.gene97637:CDS
AACTCTTCCCAAATATCCCA+AGG 0.585203 1.1:+39983941 None:intergenic
CAAGTAACTTACAGATATGG+TGG 0.592493 1.1:+39983457 None:intergenic
TTCTGCACGTCCAGGAACAA+TGG 0.595867 1.1:+39983481 None:intergenic
GCAAGATCCATACACCATCA+TGG 0.596947 1.1:+39983705 None:intergenic
ACAATGAAGATGATGATGAT+GGG 0.598315 1.1:-39982401 MS.gene97637:CDS
TGAAGGATGCTTGATCGAAG+AGG 0.615586 1.1:-39982268 MS.gene97637:CDS
CTAGAGAAGAAATCTACCAC+TGG 0.627112 1.1:-39983863 MS.gene97637:CDS
ATTGGAATTGGTGCATGGGA+AGG 0.630255 1.1:-39983821 MS.gene97637:CDS
ACGAACTACTTTCGAGCCAG+TGG 0.633329 1.1:+39983847 None:intergenic
GACGAAGAAGATGATGATGC+TGG 0.640357 1.1:-39982336 MS.gene97637:CDS
AGAAAGAATATTGATGCACA+AGG 0.643759 1.1:-39983518 MS.gene97637:CDS
CAATGAAGATGATGATGATG+GGG 0.651017 1.1:-39982400 MS.gene97637:CDS
AATAGTGGCACTTACCATGA+TGG 0.654883 1.1:-39983719 MS.gene97637:CDS
TGATGATGGGGAATGCATTG+TGG 0.656507 1.1:-39982388 MS.gene97637:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATATAACAATAAATGT+AGG + chr1.1:39983223-39983242 None:intergenic 10.0%
!!! CTTATTTAAATTTTATTTGT+TGG + chr1.1:39983520-39983539 None:intergenic 10.0%
!!! TCTTTTTTTTTTTTTTTTGT+AGG - chr1.1:39982724-39982743 MS.gene97637:intron 10.0%
!!! AAATTGGTAAATTTTAGTTT+TGG + chr1.1:39983545-39983564 None:intergenic 15.0%
!!! TCAAATGTTTTTCATAAAAT+CGG + chr1.1:39983589-39983608 None:intergenic 15.0%
!! AAAAAGATGTGAGAAATATT+TGG - chr1.1:39983106-39983125 MS.gene97637:intron 20.0%
!! AATTGATCATATGTAATGTA+AGG - chr1.1:39983252-39983271 MS.gene97637:intron 20.0%
!! ATTGATCATATGTAATGTAA+GGG - chr1.1:39983253-39983272 MS.gene97637:intron 20.0%
!! CATCTTTAAGTATTCAATAA+TGG - chr1.1:39983378-39983397 MS.gene97637:intron 20.0%
!! CTTGATTCAAGATTTAATAA+TGG - chr1.1:39982900-39982919 MS.gene97637:intron 20.0%
!!! CAACATAATTTTTTTTGAAG+AGG + chr1.1:39982880-39982899 None:intergenic 20.0%
!!! CCTATTTTTATCTTATTGTT+GGG - chr1.1:39983406-39983425 MS.gene97637:intron 20.0%
!!! TCCTATTTTTATCTTATTGT+TGG - chr1.1:39983405-39983424 MS.gene97637:intron 20.0%
!!! TGTTTTCATCTCAATAAAAT+TGG + chr1.1:39983561-39983580 None:intergenic 20.0%
! AAACAAATCGTATTCAAGAA+TGG + chr1.1:39982315-39982334 None:intergenic 25.0%
! AATCAAGTAACTTACAGATA+TGG + chr1.1:39982840-39982859 None:intergenic 25.0%
! AATTAGAATGCAAAATGAGT+TGG + chr1.1:39983787-39983806 None:intergenic 25.0%
! ATCTTTGATAAGAAATCCTT+TGG - chr1.1:39982628-39982647 MS.gene97637:intron 25.0%
! ATGTTAATGAGAAAAACAAC+CGG + chr1.1:39982940-39982959 None:intergenic 25.0%
! ATTGAACATGGAAAAAGTTA+AGG + chr1.1:39983614-39983633 None:intergenic 25.0%
! CCCAACAATAAGATAAAAAT+AGG + chr1.1:39983409-39983428 None:intergenic 25.0%
! GTTTCATGAAGAGTTATTAT+TGG - chr1.1:39984071-39984090 MS.gene97637:CDS 25.0%
! TAATACAGAGTCTGATTATA+AGG - chr1.1:39983077-39983096 MS.gene97637:intron 25.0%
! TGTAGAACTTACAAATTTGT+TGG + chr1.1:39983166-39983185 None:intergenic 25.0%
! TTAAGGTAGTGAAGAAATAT+AGG + chr1.1:39982406-39982425 None:intergenic 25.0%
!! ATTCTTGAATACGATTTGTT+TGG - chr1.1:39982314-39982333 MS.gene97637:CDS 25.0%
!! CATTCTTGATTTGATATGTA+GGG - chr1.1:39982970-39982989 MS.gene97637:intron 25.0%
!!! GAACTTCACATTTTATTTCT+GGG - chr1.1:39983656-39983675 MS.gene97637:intron 25.0%
!!! GTTTTTTAACCTATTGAACA+TGG + chr1.1:39983626-39983645 None:intergenic 25.0%
!!! TAACTTTTTCCATGTTCAAT+AGG - chr1.1:39983614-39983633 MS.gene97637:intron 25.0%
AAAGAATCCTTGGGATATTT+GGG - chr1.1:39982343-39982362 MS.gene97637:CDS 30.0%
AAATATCCCAAGGATTCTTT+AGG + chr1.1:39982343-39982362 None:intergenic 30.0%
AATATCCCAAGGATTCTTTA+GGG + chr1.1:39982342-39982361 None:intergenic 30.0%
AATGGATACATTTCAAGATC+CGG - chr1.1:39982918-39982937 MS.gene97637:intron 30.0%
AGAAAGAATATTGATGCACA+AGG - chr1.1:39982773-39982792 MS.gene97637:intron 30.0%
GATGATAGTAACATACTAAC+TGG + chr1.1:39983362-39983381 None:intergenic 30.0%
TAAAGAATCCTTGGGATATT+TGG - chr1.1:39982342-39982361 MS.gene97637:CDS 30.0%
TCTCAATTCTTCTTCAAGTA+AGG - chr1.1:39982205-39982224 MS.gene97637:CDS 30.0%
TCTGCATAGAACATAATTGT+TGG + chr1.1:39982750-39982769 None:intergenic 30.0%
TGGACTTTATAGGACAAATA+TGG - chr1.1:39983053-39983072 MS.gene97637:intron 30.0%
TTCTTATCAAAGATTTGTCG+AGG + chr1.1:39982622-39982641 None:intergenic 30.0%
! ACAATGAAGATGATGATGAT+GGG - chr1.1:39983890-39983909 MS.gene97637:CDS 30.0%
! CGTAGTTTAATCTTTTGACA+TGG + chr1.1:39983727-39983746 None:intergenic 30.0%
! GCATTCTTGATTTGATATGT+AGG - chr1.1:39982969-39982988 MS.gene97637:intron 30.0%
! TCAAGAATGGTGATGTAATT+GGG + chr1.1:39982302-39982321 None:intergenic 30.0%
! TTCAAGAATGGTGATGTAAT+TGG + chr1.1:39982303-39982322 None:intergenic 30.0%
!! AATCAAATGGTGGACTTTAT+AGG - chr1.1:39983043-39983062 MS.gene97637:intron 30.0%
!! AATTGGGAAGAGATTTGTTA+TGG + chr1.1:39982286-39982305 None:intergenic 30.0%
!! CGAACTTCACATTTTATTTC+TGG - chr1.1:39983655-39983674 MS.gene97637:intron 30.0%
!! GGATTTTGTTGTAGAGAAAT+CGG + chr1.1:39982265-39982284 None:intergenic 30.0%
!! TGTTTTCGATTTGAGAATAG+TGG - chr1.1:39982557-39982576 MS.gene97637:intron 30.0%
!!! CTCTTGCATTCTCATTTTTT+AGG - chr1.1:39983834-39983853 MS.gene97637:CDS 30.0%
!!! GAAAACATTTTTTCACACCA+AGG + chr1.1:39982544-39982563 None:intergenic 30.0%
AAAAACAAGAACCAACTCCT+TGG - chr1.1:39982524-39982543 MS.gene97637:intron 35.0%
AGTGGTAGATTTCTTCTCTA+GGG + chr1.1:39982429-39982448 None:intergenic 35.0%
CAAGTAACTTACAGATATGG+TGG + chr1.1:39982837-39982856 None:intergenic 35.0%
GTAGTTCGTTCTATTGGAAT+TGG - chr1.1:39982458-39982477 MS.gene97637:intron 35.0%
TCATCATCATCTTCATTGTC+AGG + chr1.1:39983889-39983908 None:intergenic 35.0%
TCGAAAGTAGTTCGTTCTAT+TGG - chr1.1:39982452-39982471 MS.gene97637:intron 35.0%
TGTATCACATTGCTTACCAA+AGG + chr1.1:39982647-39982666 None:intergenic 35.0%
TTCTATTGGAATTGGTGCAT+GGG - chr1.1:39982466-39982485 MS.gene97637:intron 35.0%
! AGAATGCAAGAGCACAATAA+GGG + chr1.1:39983826-39983845 None:intergenic 35.0%
! CAATGAAGATGATGATGATG+GGG - chr1.1:39983891-39983910 MS.gene97637:CDS 35.0%
! GACAATGAAGATGATGATGA+TGG - chr1.1:39983889-39983908 MS.gene97637:CDS 35.0%
!! AGATTTCTTCTCTAGGGTTA+AGG + chr1.1:39982423-39982442 None:intergenic 35.0%
!! CAGGTTGAACTATTGGATTT+TGG + chr1.1:39983870-39983889 None:intergenic 35.0%
!! TCATTGTCAGGTTGAACTAT+TGG + chr1.1:39983877-39983896 None:intergenic 35.0%
!!! ATTTTTTCACACCAAGGAGT+TGG + chr1.1:39982538-39982557 None:intergenic 35.0%
AAATGACGACGAAAGCTTTG+TGG - chr1.1:39983933-39983952 MS.gene97637:CDS 40.0%
AACTCTTCCCAAATATCCCA+AGG + chr1.1:39982353-39982372 None:intergenic 40.0%
AATAGTGGCACTTACCATGA+TGG - chr1.1:39982572-39982591 MS.gene97637:intron 40.0%
AGCTCCTTTGCAAAAATCTC+AGG + chr1.1:39983991-39984010 None:intergenic 40.0%
CAGTGGTAGATTTCTTCTCT+AGG + chr1.1:39982430-39982449 None:intergenic 40.0%
CTAAGAATGGTTGTCTCTCT+TGG + chr1.1:39983132-39983151 None:intergenic 40.0%
CTAGAGAAGAAATCTACCAC+TGG - chr1.1:39982428-39982447 MS.gene97637:CDS 40.0%
GAAAGAAAGACCATTGTTCC+TGG - chr1.1:39982800-39982819 MS.gene97637:intron 40.0%
GAACCAAGAAGCGAATCAAA+TGG - chr1.1:39983030-39983049 MS.gene97637:intron 40.0%
GAGCTGATAGAGATGAATGA+AGG - chr1.1:39984006-39984025 MS.gene97637:CDS 40.0%
GTTCTATTGGAATTGGTGCA+TGG - chr1.1:39982465-39982484 MS.gene97637:intron 40.0%
TGGGATATTTGGGAAGAGTT+TGG - chr1.1:39982353-39982372 MS.gene97637:CDS 40.0%
TGGTACCCTAAAGAATCCTT+GGG - chr1.1:39982334-39982353 MS.gene97637:CDS 40.0%
TTGGTACCCTAAAGAATCCT+TGG - chr1.1:39982333-39982352 MS.gene97637:CDS 40.0%
! ATGATGATGCTGGTATGTCT+TGG - chr1.1:39983965-39983984 MS.gene97637:CDS 40.0%
! GAGAATGCAAGAGCACAATA+AGG + chr1.1:39983827-39983846 None:intergenic 40.0%
! TTGGCCTGAGATTTTTGCAA+AGG - chr1.1:39983984-39984003 MS.gene97637:CDS 40.0%
ATGCTTGATCGAAGAGGAAG+AGG - chr1.1:39984029-39984048 MS.gene97637:CDS 45.0%
ATTGGAATTGGTGCATGGGA+AGG - chr1.1:39982470-39982489 MS.gene97637:intron 45.0%
CCAAGAAGCGAATCAAATGG+TGG - chr1.1:39983033-39983052 MS.gene97637:intron 45.0%
GCAAGATCCATACACCATCA+TGG + chr1.1:39982589-39982608 None:intergenic 45.0%
GGCTTCTTCGAGTCTAAGAA+TGG + chr1.1:39983145-39983164 None:intergenic 45.0%
TGAAGGATGCTTGATCGAAG+AGG - chr1.1:39984023-39984042 MS.gene97637:CDS 45.0%
TGGGAAGGTGAAGAAGATAC+TGG - chr1.1:39982485-39982504 MS.gene97637:intron 45.0%
! CCACCATTTGATTCGCTTCT+TGG + chr1.1:39983036-39983055 None:intergenic 45.0%
! GACGAAGAAGATGATGATGC+TGG - chr1.1:39983955-39983974 MS.gene97637:CDS 45.0%
! GCACTTACCATGATGGTGTA+TGG - chr1.1:39982579-39982598 MS.gene97637:intron 45.0%
!! TGATGATGGGGAATGCATTG+TGG - chr1.1:39983903-39983922 MS.gene97637:CDS 45.0%
ACGAACTACTTTCGAGCCAG+TGG + chr1.1:39982447-39982466 None:intergenic 50.0%
TTCTGCACGTCCAGGAACAA+TGG + chr1.1:39982813-39982832 None:intergenic 50.0%
ATGGTGGTTTCTGCACGTCC+AGG + chr1.1:39982821-39982840 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 39982200 39984113 39982200 ID=MS.gene97637
chr1.1 mRNA 39982200 39984113 39982200 ID=MS.gene97637.t1;Parent=MS.gene97637
chr1.1 exon 39983664 39984113 39983664 ID=MS.gene97637.t1.exon1;Parent=MS.gene97637.t1
chr1.1 CDS 39983664 39984113 39983664 ID=cds.MS.gene97637.t1;Parent=MS.gene97637.t1
chr1.1 exon 39983469 39983567 39983469 ID=MS.gene97637.t1.exon2;Parent=MS.gene97637.t1
chr1.1 CDS 39983469 39983567 39983469 ID=cds.MS.gene97637.t1;Parent=MS.gene97637.t1
chr1.1 exon 39982200 39982457 39982200 ID=MS.gene97637.t1.exon3;Parent=MS.gene97637.t1
chr1.1 CDS 39982200 39982457 39982200 ID=cds.MS.gene97637.t1;Parent=MS.gene97637.t1
Gene Sequence

>MS.gene97637

ATGGCTCTCAATTCTTCTTCAAGTAAGGATTTCAAATTCACACTCAGAGATGATGAACTTCTCCGATTTCTCTACAACAAAATCCATAACAAATCTCTTCCCAATTACATCACCATTCTTGAATACGATTTGTTTGGTACCCTAAAGAATCCTTGGGATATTTGGGAAGAGTTTGGAGCTTCTCGCTCATATTGTGAAAAAGACCTATATTTCTTCACTACCTTAACCCTAGAGAAGAAATCTACCACTGGCTCGAAAGTAGTTCGTTCTATTGGAATTGGTGCATGGGAAGGTGAAGAAGATACTGGAAAAATTATAGTTGCAAAAAACAAGAACCAACTCCTTGGTGTGAAAAAATGTTTTCGATTTGAGAATAGTGGCACTTACCATGATGGTGTATGGATCTTGCATGAATATAGCCTCGACAAATCTTTGATAAGAAATCCTTTGGTAAGCAATGTGATACATTTACATTATTATTTTTTCTTGTTTTTTGAAGCATATTTATATAATTTGATTTTGTATCTTTTTTTTTTTTTTTTGTAGGCCAACAATTATGTTCTATGCAGATTTAGAAAGAATATTGATGCACAAGGTAAAGAAAGAAAGACCATTGTTCCTGGACGTGCAGAAACCACCATATCTGTAAGTTACTTGATTAATTATGTTGCTTGATTCCTCTTCAAAAAAAATTATGTTGCTTGATTCAAGATTTAATAATGGATACATTTCAAGATCCGGTTGTTTTTCTCATTAACATTGTTCTTTTGCATTCTTGATTTGATATGTAGGGAAATAAAAATTCGTTGACAAAATATATAATTTATAAAGAACCAAGAAGCGAATCAAATGGTGGACTTTATAGGACAAATATGGTTAATACAGAGTCTGATTATAAGGAAGAAGAAAAAGATGTGAGAAATATTTGGCCAAGAGAGACAACCATTCTTAGACTCGAAGAAGCCAACAAATTTGTAAGTTCTACATTCGTTGTTGTTTTTCTTATTTTTTAAAGAAAGTCCTACATTTATTGTTATATAATATCTAATAACAATTGATCATATGTAATGTAAGGGTTTATTAGAGTTACTAGTACTACAGTTTTTAGAGACACTGCTCTTTATAAATCATTGTGAATTTTTAAAATTTAGATCAATTACCAGTTAGTATGTTACTATCATCTTTAAGTATTCAATAATGGATTTTCCTATTTTTATCTTATTGTTGGGTTAAAATTTTTTTTTCATTTCTATAATAGTGTATTTTTTAGTCTCATCAAAAAACTAGACTCATTATTTTTGCAAATTTTAAAAAATACCAACAAATAAAATTTAAATAAGAACCAAAACTAAAATTTACCAATTTTATTGAGATGAAAACATGAGTCCGATTTTATGAAAAACATTTGAATCCTTAACTTTTTCCATGTTCAATAGGTTAAAAAACAAATATAGTCGAACTTCACATTTTATTTCTGGGTCTAACATTATACTTGTTTGAAGTTAAATATATTTTTAATCACATCCATGTCAAAAGATTAAACTACGAATTATTATAATAATTATAACAATAGAGTAAATCAGACCAACTCATTTTGCATTCTAATTGTATTGATCATTCAATCCCTTATTGTGCTCTTGCATTCTCATTTTTTAGGAGAATATTAACCAAAATCCAATAGTTCAACCTGACAATGAAGATGATGATGATGGGGAATGCATTGTGGACAAAGAAAATGACGACGAAAGCTTTGTGGACGAAGAAGATGATGATGCTGGTATGTCTTGGCCTGAGATTTTTGCAAAGGAGCTGATAGAGATGAATGAAGGATGCTTGATCGAAGAGGAAGAGGTTCAAATGATTCAAAATAAGTTTCATGAAGAGTTATTATTGGATAATACTAATATAAAATAA
Protein sequence

>MS.gene97637.t1

MALNSSSSKDFKFTLRDDELLRFLYNKIHNKSLPNYITILEYDLFGTLKNPWDIWEEFGASRSYCEKDLYFFTTLTLEKKSTTGSKVVRSIGIGAWEGEEDTGKIIVAKNKNQLLGVKKCFRFENSGTYHDGVWILHEYSLDKSLIRNPLANNYVLCRFRKNIDAQGKERKTIVPGRAETTISENINQNPIVQPDNEDDDDGECIVDKENDDESFVDEEDDDAGMSWPEIFAKELIEMNEGCLIEEEEVQMIQNKFHEELLLDNTNIK